-11.9 kb

-4.2 kb

-5.6 kb

Gfi1+/1b neo

Gfi1+/+

Gfi1+/1b

Gfi11b/1b

Gfi1+/1b

Gfi1+/+

-11.9 kb

- 4.2 kb

Gfi1+/+

Gfi1+/1b

-14 kb

- 8 kb

BAGfi1+/+ Gfi1+/1b Gfi11b/1b

Gfi1(55 kDa)

Gfi1b(37 kDa)

loading control

C

loading control

Supp. Figure 1

Gfi1+/+

Gfi1P2A/P2A

- 15 kb

- 8 kb

BGfi1

+/P2A

Gfi1+/+

- 15 kb

- 8 kb

Gfi1+/P2A

Gfi1P2A/P2A

Gfi1+/+

Gfi1-/-

Gfi1(55kDa)

loading control

A

Supp. Figure 2

∆ Rn

cycle

0 5 10 15 20 25 30 35 40

Amplification Plot Gfi11b/1b

1.000 E-4

1.000 E-3

1.000 E-2

1.000 E-1

1.000

1.000 E+1

Gfi1

Gfi1b

1.000 E-4

1.000 E-3

1.000 E-2

1.000 E-1

1.000

1.000 E+1

Gfi1Gfi1b

0 5 10 15 20 25 30 35 40

Amplification Plot Gfi1+/+

A

B

Gfi1

Gfi1b

GAPDH

Gfi1+/+ Gfi11b/1b H2O

Supp. Figure 3

IP: anti Gfi1bWe: anti PIAS3

PIAS3

175

8362

47.5

IP: anti Gfi1bWe: anti Gfi1b

Inpu

t

IP a

nti-G

ST

IP a

nti-G

fi1b

Gfi1b

IgG

8362

47.5

32.5

Supp. Figure 4

Table 1 Differential cell counts of wild type, Gfi1-/- , Gfi1P2A/P2A and Gfi11b/1b mice

Supp. Table 1

significant

bone marrow

cell counts (%)Gfi1+/+

(n=7)

Gfi1-/-

(n=8)

t-test Gfi11b/1b

(n=7)

t-test Gfi1P2A/P2A

(n=3)

t-test

lymphocytes 22 ± 11 7 ± 7 0.01∗ 19 ± 8 0.617 6 ± 3 0.055

m yeloblasts 4 ± 2 4 ± 5 1 7 ± 3 0.063 9 ± 8 0.214

prom yeloblasts 4 ± 2 2 ± 2 0.075 5 ± 2 0.56 4 ± 5 0.902

m yelocytes 7 ± 4 4 ± 2 0.06 13 ± 6 0.053 9 ± 7 0.622

m etam yelozytes 20 ± 7 4 ± 2 4.1x10-5 ∗ 13 ± 4 0.049 ∗ 4 ± 1 0.006 ∗

granulocytes 36 ± 13 2± 3 5.4x10-6 ∗ 28 ± 7 0.202 2 ± 2 0.002 ∗

m onocytes 2 ± 2 16 ± 24 0.173 7 ± 4 0.014 ∗ 13 ± 1 2.7x10-5 ∗

atypical m onocytes 1 ± 3 57 ± 21 1.1x10-5 ∗ 2 ± 6 0.688 48 ± 24 0.002 ∗

eosinophils 2 ± 2 1 ± 1 0.449 1 ± 1 0.446 1 ± 1 0.705

erythroid 3 ± 5 3± 3 0.75 5 ± 6 0.484 3 ± 4 0.906

blood

cell counts (%)

Gfi1+/+

(n=9)

Gfi1-/-

(n=5)

t-test Gfi11b/1b

(n=7)

t-test Gfi1P2A/P2A

(n=3)

t-test

lym phocytes 80 ± 6 54 ± 22 0.005 ∗ 81 ± 9 0.802 51 ± 21 0.002 ∗

granulocytes 15 ± 6 0 ± 1 9.1x10-5 ∗ 10 ± 5 0.067 2 ± 3 0.003 ∗

m onocytes 4 ± 2 24 ± 22 0.016 ∗ 8 ± 5 0.046 ∗ 23 ± 18 0.005 ∗

atypical m onocytes 0 20 ± 8 6.5x10-6 ∗ 0 ± 1 0.121 23± 7 3.5x10-7 ∗

eosinophils 1 ± 1 1 ± 2 0.979 1 ± 1 0.749 0 ± 1 0.32