.

( ) PH PH .

. (( )).

..- - .

.

. .

Purification Of Enzymes : 1- Molecular Precipitation 2- Adsorption .3- Polymerization4- Centrifugation.5- Electrophoresis .-

IU : 1 30 .

:

. . :. . . ( )

( ) Cofactor . (CO- enzymes) . B.

. Coenzyme Prosthetic group . Apoenzyme Holoenzyme .

:

:

, ACTIVE SITE ( ) Substrate . , , .

Specificity of enzymes : (Absolute specificity) ( (Urease Low Specificity Lipase .Stereo-chemical Specificity

- - .- .- .- .- (pro Enzymes) .- PH ( ) .- ( ) .- ( ) .

:

. (( )) "ase " ( ) . (( ))"ase " (( )) .

1961 "ase " a Systematic Code number for each enzyme [ E . C] 4 .

(3.1.1.3( {3} (3) {1} (1) (1.3) {1} ( ) (3.1.1) {3} 3.1.1.3.

6 : 1961 .

_1 Oxidoreductases . : . . . .

) Phenol-oxidase: Phenol-oxidase (o-Diphenoloxidase:EC1.10.3.1) (p- Diphenoloxidase : EC 1.10.3.2) . Quinone Melanin

) (Catalase ):

Catalase (EC 1. 11.1.6) (H2O2) : 2 H2O2 2 H2O + O2 Catalase

) Peroxidase : Peroxidase (EC 1.11.1.7) (H2O2) : Peroxidase AH2 + H2O2 A + 2H2O

_2 Transferases . . (EC 2.4.1.1) Phosphorylase Dextrins ( ) .

3- (Hydrolases) . () (EC 3.2.1.26) Invertase (Glucose 1-2 Fructose) ( ) ( ) :

3 _ ( ) Hydrolases .

4- Lyases ( ) (Aldolase) 16

5- Isomerases .

6- Ligases (ATP)

. _

. ( -20 ) .

(PH )- PH .

5- Inhibitors (Competitive inhibitor) . .

2. (Non Competitive Inhibitor) . .