(-477 to- 751)

1
(-477 to- 751) (A) ATG5 ( -2110 to -31 40) LTR CpG islan d (+ 568 t o -1 10 ) SINE upplemental Figure 3 (B) AIM1 CpG island (-980 to -1360) CpG island (+30 to +380) CpG isl and (+610 to +97 0) Set 1 2 3 1.HANK1 2.NK92 3.YT 4.SNK1 5.M.SssI treated lymphocyte DNA 6.Bisulfite treated lymphocyte DNA 7.No template 8.NKYS 9.KHYGI 10.SNK6 11.KAI3 12.M.SssI treated lymphocyte DNA 13.Bisulfite treated lymphocyte DNA 14.No template Methylation Specific PCR 400 300 300 200 400 300 1 2 3 4 5 6 8 9 10 11 12 13 14 1 2 3 4 5 6 8 9 10 11 12 13 14 Set 1 Set 2 Set 3 DNA ladder Met UnM Met UnM Met UnM Met UnM Met UnM Met UnM Met UnM 1 2 3 4 5 6 8 9 10 11 12 13 Methylation Specific PCR DNA ladder SINE 2 3 4 5 6 9 10 11 12 13 300 200 LTR 400 300 2 3 4 5 6 7 10 11 12 13 14 Met UnM Met UnM Met UnM Met UnM Met UnM Met UnM Met UnM 2 3 4 5 6 7 9 10 11 12 13 14

Upload: hayes

Post on 12-Jan-2016

29 views

Category:

Documents


1 download

DESCRIPTION

Supplemental Figure 3. (A) ATG5. (B) AIM1. CpG island (-980 to -1360). CpG island (+30 to +380). CpG island (+610 to +970). (-2110 to -3140). (-477 to- 751). CpG island (+ 568 to -110). SINE. Set 1 2 3. LTR. Methylation Specific PCR. - PowerPoint PPT Presentation

TRANSCRIPT

Page 1: (-477  to- 751)

(-477

to-

751

)(A) ATG5

(-211

0 to

-314

0)

LT

RCpG

isla

nd

(+ 5

68 t

o -1

10)

S

INE

Supplemental Figure 3

(B) AIM1

CpG

isla

nd

(-980

to

-136

0)

CpG isla

nd

(+30

to +

380)

CpG isla

nd

(+61

0 to

+97

0)

Set 1 2 3

1.HANK12.NK923.YT4.SNK15.M.SssI treated lymphocyte DNA6.Bisulfite treated lymphocyte DNA 7.No template8.NKYS9.KHYGI10.SNK611.KAI312.M.SssI treated lymphocyte DNA13.Bisulfite treated lymphocyte DNA 14.No template

Methylation Specific PCR

400 300

300 200

400 300

1 2 3 4 5 6 7

8 9 10 11 12 13 14

1 2 3 4 5 6 7

8 9 10 11 12 13 14

Set 1

Set 2

Set 3

DN

A lad

der

Met

UnM

Met

UnM

Met

UnM

Met

UnM Met

UnM

Met

UnM

Met

UnM

1 2 3 4 5 6 7

8 9 10 11 12 13 14

Methylation Specific PCR

DN

A la

dder

SINE

1 2 3 4 5 6

8 9 10 11 12 13

300 200

LTR400

3001 2 3 4 5 6 7

8 9 10 11 12 13 14

Met

UnM

Met

UnM

Met

UnM

Met

UnM

Met

UnM

Met

UnM

Met

UnM

1 2 3 4 5 6 7

8 9 10 11 12 13 14