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ANIMAL AGRICULTURE AND VETERINARY MEDICINEQuarterly research and practice journal

Published since may 2010

Journal founder – Educational establishment «Belarusian State Order of thectober Revolution and Order of the Red Banner of Labour Agricultural Academy»

Zooengineering faculty

According to the order of the High Attestation Commission of the Republic of Belarus the jour-nal has been included in the list of scientific works for publishing results of theses on agriculturaland veterinary sciences

Editorial Board:

Managing Editor – Kurdeko A. P., Doctor of Veterinary Sciences, Professor;ssistant Managing Editor – Mikulich E. L., Candidate of Veterinary Sciences, Docent;

Executive Secretary – Tsikunova O. G., Candidate of Sciences in Agriculture, Docent;Editor and corrector – Savchitz E. H.;English Translation – Liakhnovitch T. L., Candidate of Sciences, Docent.

1.

. ., . .………………………………………………………………………………… 5

. ., . ., . ., . .……………………………………………..10

. ., . . (Bison Bonasus L) ……… 16. ., . .

…………………………………………………………………………..20

2.

. ., . ., . ., . . , « »

……………………………………………………………………………….. 25. . ………...31

. ., i . ., . ., T. A. i i i……………………………………….37

. ., . ., . ., . ., . .……………41

. ., . . . ………………………………………………………………………….46

. . « » ……………………………………………………………………………………..51

3.

. . 7- ………………….55

CONTENTS1. ANIMAL SCIENCE

Ladyka L. N., Kiselev A. B. Carcass and slaughter traits of young male goats of the Ukranianpopulation of dairy goats……………………………………………………………………………………..5

Barulin N. V., Yurchenko, T. P., Shalak M. V., Sadomov N. A. Rating mobility sturgeonsperm under akvakultury……………………………………………………………………………………10

Yevtushevsky N. N., Mamenko A. M. On conservation of european bison (Bison Bonasus L)in Ukraine…………………………………………………………………………………………………..16

Bondareva M. S., Seryakov I. S. Effectiveness of enzymatic agents in feeding young pigs…………20

2. VETERINARY MEDICINE

Kukhtina O. N., Medvedev G. F., Gavrichenko N. I., Sivakov A. A. Development, controlmethods and administration of antimicrobial «Fertilifil K» to increase conception rate of cows……………25

Tumilovich G. A. Morphostructural features of rumen mucous membrane in calves……………….. 31Khlebus N. K., Petrovsky S. V., Makaruk M. A., Zdanovich T. A. Reproductive qualities

of sows with metabolic disorders and liver damage………………………………………………………..37Gromov I. N., Selikhanova M. K., Zhurov D. O., Aliev A. S., Emelyanova S. A.

Pathomorphology of bone marrow in SPF-embryos and chickens with infectious anaemia……………….41Yurko P. S., Kulibaba R. A. Goose virus enteritis. Differential diagnostics and ethioprophylaxis…..46Zamaziy A. A. Effect of hypoxia on the «maturity» of lung surfactant system in newborn calves……51

3. THE KNOWN AND THE UNKNOWN

Barulin N. V. 7th International symposium on sturgeon in Canada………………………………….. 55

5

1.

639.303.45:535.21: 577.3

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,. , ., , 40021

08.11.2013)

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Summary. Carcass and slaughter traits of eight-month-old male goats and castrated-goats of the Ukrainian population of dairygoats grown for meat have been studied. It has been established that the main factor which positively influences the slaughter traitsand quality of meat is a «diet factor». Castration only decelerates the formation of carcass and slaughter traits of the goats.

Key words: young male goats, castration, slaughter, body carcass, cuts.

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1. . – . –, 1984. – 58 .

2. , . . / . . //: . – 2011. – 7. – . 184–191.

3. Bonvi l l ani, A. Meat quality of Criollo Cordobes goat kids produced under extensive feeding conditions. Effects of sex andage/weight at slaughter / A. Bonvillani [at al.] // J. Agric. Res. – Span., 2010. – V. 8. – P. 116–125.

4. Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisionsof the Member States regarding the protection of animals used for experimental and other scientific purposes.

5. Colomber -Rocher , F. Carcass composition of New Zealand Saanen goats slaughtered at different weights / F. Colomber-Rocher, A. H. Kirton, G. J. K. Mercer // Small Rumin. Res. – 1992. – V. 2. – P. 7161–7173.

6. Oman, J. S. Effect of Breed-Type and Feeding Regimen on Goat Carcass Traits / J. S. Oman, D. F. Waldron, D. B. Griffin //Journal of animal science. – 1999. – . 3215–3218.

7. Werdi Prat iwi, N. M. Feral goats in Australia: A study on the quality and nutritive value of their meat / N. M. Werdi Prati-wi, P. J. Murray, D. G. Taylor // Meat Science 75. – 2007. – . 168–177.

8. Wi l li am, B. Carcass Evaluatio Fabrication / B. William, R. Chancellor, D. Boethel // Meat Goat Selection, Guide. Pub. 2951(3M) 01/08 Rev.

9. http://gastronoma.net/ua/adwards/catalog/zhivotnovodstvo-kozovodstvo/.

10

639.3.034.2

. . , . . , . . , . .

« ». , , , 213407

09.12.2013)

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Summary. The article presents the results of surveys measuring sperm motility in sturgeon aquaculture conditions by computeranalysis. Results of measurements of the mobility on such parameters as: curvilinear velocity, straight line velocity, average pathvelocity, oscillation, linearity.

Key words: aquaculture, sperm, sturgeon fish, sperm quality, males.

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1. , . . . . 2: : / . . , . . ; . . . . – : , 2008. –

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3. / . . [ .]. – M. – : « « »,2006. – 145 .

4. [ ] / – : http://www.mmcatalog.com/index.html. – :08.10.2013.

5. /. . [ .] // . . – 2006. – . 8. – . 66–69.

6. , . . / . . . – « », 1972. – 280 .7. A preliminary study of oscillating electromagnetic field effects on human spermatozoon motility / R. Iorio [et al.] // Bioelec-

tromagnetics. – 2007. – Vol. 28. – 1. – P. 72–75.8. Chebanov, M. S. Sturgeon hatchery manual / M. S. Chebanov, E. V. Galich. – FAO, Ankara. – 2013. – 303 p.9. Donoghue, A. M. Effects of water- and lipid-soluble antioxidants on turkey sperm viability, membrane integrity, and motili-

ty during liquid storage / A. M. Donoghue, D. J. Donoghue // Poultry Science. – 1997. – Vol. 76. – 10. – P. 1440–1445.10. Effect of 655-nm diode laser on dog sperm motility / M. I. Corral-Baqués [et al.] // Lasers Med Sci. – 2005. – Vol. 20. – 1. –

P. 28–34.11. Effects of glucose and fructose on motility patterns of dog spermatozoa from fresh ejaculates / T. Rigau [et al.] // Therioge-

nology. – 2001. – Vol. 56. – P. 801–815.12. Factors affecting sperm motility. III. Influence of visible light and other electromagnetic radiations on human sperm velocity

and survival / A. Makler [et al.] // Fertil Steril. – 1980. – Vol. 33. – 4. – P. 439–444.13. Fauvel , C. Evaluation of fish sperm quality / C. Fauvel, M. Suquet, J. Cosson // Journal of Applied Ichthyology. – 2010. –

Vol. 26, Iss. 5. – P. 636–643.14. Improvement of stored turkey semen quality as a result of He-Ne laser irradiation / N. Iaffaldano [et al.] // Anim Reprod Sci. –

2005. – Vol. 85. – 3–4. – P. 317–325.15. In vitro capacitation and acrosome reaction of dog spermatozoa can be feasibly attained in a defined medium without glucose /

J. L. Albarracin [et al.] // Reprod Dom Anim. – 2004. – Vol. 39. – P. 1–7.16. Influence of Visible Light and Ultraviolet Irradiation on Motility and Fertility of Mammalian and Fish Sperm / T. Zan-Bar [et

al.] // Photomedicine and Laser Surgery. – 2005. – Vol. 23. – 6. – P. 549–555.17. Low energy narrow band non-coherent infrared illumination of human semen and isolated sperm / R. Singer [et al.] // Andro-

logia. – 1991. – Vol. 23. – 2. – P. 181–184.18. Radiation exposure exerts its adverse effects on sperm maturation through estrogen-induced hypothalamohypophyseal axis

inhibition in rats / M. J. Makinta [et al.] // African Zoology. – 2005. – Vol. 40. – 2. – P. 243 -251.19. WHO laboratory manual for the examination and processing of human semen. Fifth edition. World Health Organization. –

2010. – 271 p.20. Glantz, S. A. Primer of Biostatistics. / S. A. Glantz. – McGraw-Hill: New York, 2011. – 320 p.21. Hi ll , T. Statistics: Methods and Applications / T. Hill, P. Lewicki. - StatSoft, Inc., 2005. – 800 p.

16

599.735.3

(BISON BONASUS L)

. . , . .

,. , ., , 62341

29.11.2013)

. . . .

, . .

.: , , , , .

Summary. The article is devoted to the European bison conservation in Ukraine. The population of this species has collapsed.A number of hunting farms supply overstated data on the population size. The role of the state hunting farms in the processes underconsideration has been shown and their indifference to the problem of bison conservation has been pointed out. The use of enclosurehousing to preserve reacclimatisants has been proposed. The problem of bison preservation is supposed to be solved at the statelevel.

Key words: poaching, bison, hunting farm, population, enclosure housing.

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20

636.4:636.085.72

. . , . .

« ». , ., , 213407

29.11.2013)

. . « -400 » « »

. « » 100 . « »

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: , , « -400 », « », ,.

Summary. Influence of fermental additives on digestibility of nutrients, at young growth of pigs is studied. Introduction in com-pound feeds of fermental additives «Belvitazim-the 400th granulate» and «Fitaza» allows to increase assimilation practically allnutrients of a diet and to improve average daily . The best indicators are received when using an additive of «Fitaz» in adose of 100 g/t. When using a fermental additive «Fitaz» digestibility of nutrients was higher, than in control group. Digestibility ofsolid was more, than at control analogs, on 6,98 items ( 0,05), crude fat – on 8,59 ( 0,05) and a crude protein – on 2,72 pct.

Keywords: pigs, fermental feed additives «Belvitazim-400 Granulate», «Fitaza», digestibility, average daily gain.

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-26 : (30 %), (20 %), (19,44 %), (10 %), (10 %), (5 %), (3,1 %), (0,56 %), -4-1 (1 %), (0,5 %), (0,4 %).

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8,77 %, – 2,72 % – 6,98 %. -, .

24

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1- 2- 3-71,49±0,3 74,98±1,3 76,48±0,8*

± – +4,89 +6,9860,43±0,78 61,72±0,38 65,62±0,61*

± – +2,14 +8,5975,74±0,88 77,03±1,52 77,80±0,66

± – +1,71 +2,7269,13±0,52 73,17±0,66*** 75,19±0,22***

± – +5,85 +8,77

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1. , . / . // . – 2003. – 9. – . 32–34.2. , . « » / . , . , . //

. – 1998. – 3. – . 33–34.3. / . . [ .] // . – 1998. – 8. – . 38–39.4. , . . : : / . . ,

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3.9. / . . // . – 2011. – 2. – . 44–46.10. , . / . , . // . –

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25

2. 632.22/.28:636.082.454:619:615.28

, « »

. . , . . , . .

« »,. , , 213407

. .

« ». , , 220013

( 21.10.2013)

. « » . .

0,375 ( ), 1,5 (4 ) 10 . 2- .. ,

, . « » ( ) « » ( ) -

40 % , 5 – - 22±0,3 % 20±0,3 % (P<0,01). 6 22, – 22.

35,1 %. (77,7 %) – (50 %).

: , , , , , , .

Summary. Preparation «Fertilifil K» has been developed to integrate into bull semen diluent and increase conception rate whileinseminating cows. Four antibiotics are integrated into the preparation. Experimental batch of the preparation was produced in0.375 g (one dose) or 1.5 g (4 doses) in 10-ml-bottles.Shelf life is at least 2 years. Analytical method for identification of antibioticsand determination of their mass concentration has been developed. Microorganisms isolated from the uterus of cows in repeatedestrus were highly sensitive to the preparation.

When «Fertilifil K»(experiment) or «Poligen» (control) was added to the diluent after thawing of the frozen semen samples therewas 40 % of active sperm, and after 5-hour incubation – 22±0.3 % and 20±0.3 % (P<0,01) respectively. In the experiment coloniesgrew on agar culture medium in 6 samples of 22, in the control – in all 22 samples.

After administration of the preparation while inseminating the cows having low reproductive rate the conception rate was35.1 %. The high result was obtained after the preparation was administered to the cows in the third estrus (77.7 %) and goodenough one – in the fourth estrus (50 %).

Key words: preparation, Fertilifil K, cows, conception rate, microorganisms, semen, quality.

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Edition. 2001. W.B. Saunders Comp. Ltd. 868 . (Reprinted 2007), . 461–464.9. Bal l , P. J. H. Reproduction in cattle. Third edition / P. J. H. Ball and A. R. Peters // Blackwell publishing, 2004. – P. 178,

216–217.10. Veterinary Reproduction and Obstetrics. Ninth Edition. Edited by David E. Noakes, Timothy J. Parkinson, Gary C. W. Eng-

land. 2009. W. B. Saunders Elsevier. Ltd. – P. 463–465.11. The artificial insemination and Embryo transfer of dairy and beef cattle (including information pertaining to goats, sheep,

horses, swine, and other animals). A handbook and laboratory manual for students, herd operators, and persons involved in geneticdevelopment. Jere R. Mitchell, Gordon A. Doak. Copyright 2004 by Pearson Education, 387 p.

31

636.22/.28:611.3

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09.09.2013)

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Summary. Degree of papilla development in different departments of rumen in calves has been determined. The most distinctage-related changes of size, form and the number of papillae are noted in antrum rumenis, saccus cecus dorsalis and ventralis rume-nis. Various intensity of papilla growth in certain sacs of a rumen is attributed to the features of metabolic processes. The overallthickness of the epithelial layer of a rumen wall in calves is thinning out with age, while cornific layer of the epithelium is growing.Epithelium of antrum rumenis and the arches of dorsal and ventral sacs are exposed to the strongest cornification.

Keywords: calves, rumen, epithelium, papillae, mucous membrane, morphology, dorsal and ventral sacs, antrum rumenis.

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37

636.4.053:612.015.3:616.36-008.64

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. . I, . . , T. A.

« »,. , , 210026

( 22.11.2013)

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Summary. The paper presents the findings of research on the functional state of liver in sows at the advanced stage of gestation.It is established that renal failure, energy shortages and anemic syndrome occur in sows during the development of liver diseasecharacterized by cytolytic and hepato-depressive syndromes. With the development of these disorders in sows the number of physio-logically immature and stillborn piglets with reduced body weight increases among the newborn pigs.

Keywords: sows, liver diseases, energy deficiency, renal failure, anemia, pigs with hypotrophy.

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2. , . . / . . ,. . // i / :

. i II . . – , 1998. – . 5. – . 1. – . 92–95.3. , . . /

. . , . . , . . // « » . – , 2000. – . 36. – . 2. – . 105–107.

4. / . . . . – :, 2003. – 56 .

5. , . . : / . . . – : . – 78 .

6. , . . , / . . , . . // . – , 2002. – . 38. – . 2. – . 39–40.

7. , . . / . . ,. . // :

. . .- . ., . 25- .- . . – , 1999. – . 152–154.8. Lindemann, M. D. Supplemental folic acid: a requirement for optimizing swine reproduction / M. D. Lindemann // J. Anim.

Sci. – 1993. – Vol. 71. – 1. – . 239–246.9. Treatment of congenital lactic acidosis with dichloroacetate / W. Peter [et al.]. // Arch. Dis. Child. – 1997. – Vol.77. – 4. –

. 535–541.10. Von Bore l l , E. Stereotypic behavior and productivity of sows / E. von Borell, J. F. Hurnik // Canadian Journal of Animal

Science. – 1990. – Vol. 70. – 3. – . 953–956.11. Vybrané biochemické ukazovatele krvnej plazmy pohlavne nedospelých a pohlavne dospelých prasni iek / T. Filipejová [et al.]. //

Acta fytotechnica et zootechnica – Mimoriadne íslo Nitra, Slovaca Universitas Agriculturae Nitriae, 2009. – S. 163–169.12. Wol f, P. L. Biochemical diagnosis of liver disease / P. L. Wolf // Indian Journal of Clinical Biochemistry. – 1999. – Vol. 14,

1. – P. 59–90.13. Hepatorenal syndrome / C. K. Ng [et al.]. // Clin. Biochem. Rev. – 2007. – Vol. 28. – 1. – . 11–17.

41

619 636.598 616.34-002

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18.11.2013)

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Summary. The article is considering the difficulties of diagnosing goose viral enteritis of different etiologies. The combined ap-proach using the polymerase chain reaction, which allows accurate diagnosis is proposed. Using the polymerase chain reaction forcontrol of efficiency of parvovirus inactivation and polyomavirus contamination in the production of a vaccine against the Derzsy'sdisease is shown.

Key words: goose viral enteritis, polyomavirus, parvovirus, duplex PCR, inactivation, inactivated vaccine.

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, . . // . – 9 (118). – 2012 . – . 9–14.7. / . . . ,

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. 2089893 / . . , . . , . . [ .] //. – 1997.

9. , . . / . . // .: . – 1966. – 681 .10. A novel polyomavirus goose hemorrhagic polyomavirus is the agent of hemorrhagic nephritis enteritis of geese / J. Guerin,

J. Gelfi, L. Dubois [et al] // J. of Virol. – 2000. – V. 74(10). – P. 4523–4529.11. Detection of reticuloendotheliosis virus as a contaminant of fowl pox vaccines / A. M. Awad , H. S. Abd El-Hamid ,

A. A. Abou Rawash [et al] // Poultry Science. – 2010. – V. 89. – . 2389–2395.12. Diseases of poultry / editor-in-chief, Y.M. Saif. – 12th ed., Blackwell Publishing Ltd. – 2008. – 1409 .13. Effective inactivati n test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse transcriptase-

polymerase chain reaction / J. Zhou, G. Ji, J. Wen [et al] // Chinese Journal of Experimental and Clinical Virology. – 2008. –V. 22(6). – . 488–491.

14. Palya , V. Major viral disease of waterfowl and their control / V. Palya // International Poultry Production. – 2011. –V. 19(4). – P. 21–26.

15. Quantitative PCR: a quality control assay for estimation of viable virus content in live attenuated goat pox vaccine /D. Kallesh, M. Hosamani, V. Balamurugan [et al] // Indian Journal of Experimental Biology. – 2009. – V. 47. – . 911–915.

16. Recombinant subunit vaccine elicits protection against goose haemorrhagic nephritis and enteritis / T. Mato, Z. Penzes,P. Rueda [et al] // Avian Pathol. – 2009. – V. 38(3). – P. 233–237.

17. Safety and efficacy of an inactivated Carbopol-adjuvanted goose haemorrhagic polyomavirus vaccine for domestic geese /J. Gelfi, M. Pappalardo, C. Claverys [et al] // Avian Pathol. – 2010. – V. 39(2). – . 111–116.

18. Viral infections in goose flocks in Poland / W. Kozdrum, G. Wozniakowski, E. Samorek-Salamonowicz [et al] // Polish Jour-nal of Veterinary Sciences. – V. 15(3). – 2012. – P. 525–530.

46

619:616.98:578.831:636.5.053

-

. . , . . , . .

« « » »,. , , 210026

. . , . .

« »,. , , 196084

20.11.2013)

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Summary. The article presents the results of investigation into the morphological changes in bone marrow of SPF-embryos andchickens which were experimentally infected with anaemia. It also contains information about the impact of the virus of infectiousanemia on the indicators of myelogram and partial formulas of hematopoietic cells in the bone marrow.

Key words: SPF-embryos, chickens, pathomorphological changes, infectious anaemia, bone marrow.

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50

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, 1996. – 544 .10.

/ . . [ .]. // . – 2003. – 2. – . 66–69.11. / . [ .]. //

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51

: 619:616.24

« »

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. , ., , 40021

22.11.2013)

. , , . ,

1,98:1, , , 1,16:1.

707,00±5,00 ., , 1,20 .: , , , .

Summary. The results of these studies indicate that the composition of the amniotic fluid reflects the functional activity of thefetal lung, which synthesize and secrete in this fluid lipids. Found that in clinically healthy newborn calves ratiolecithin/sphingomyelin reaches 1,98:1 and calves, which were born in a state of hypoxia, it is only 1,16:1. In the amniotic fluid ofclinically healthy calves lipid fraction is 707,00±5,00 a.u.m. and calves born with signs of asphyxia it below 1,20 times.

Key words: pulmonary surfactant system, lecithin, sphingomyelin, hypoxia.

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(n=10)6 60,00 4 40,00 5 50,00 5 50,00

(n=18)14 77,78 4 22,22 11 61,11 7 38,39***

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(n=18)12 66,67 6 33,33 10 55,56 8 44,44

45 70,23 18 29,77 36 56,37 27 43,63

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473,07±3,0662,68±2,88

141,67±3,54 92,14±2,96183,33±4,21 121,43±3,52**159,00±4,00 92,14±2,9292,59±3,00 104,68±3,03**

1,98:1 1,16:1165,00±2,00 100,00±2,00**

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54

3 . , (M±m, %)

( , n=3) (n=9) 14:0 27,00±0,67 11,28±0,44** 14:1 0,83±0,16 0,48±0,03** 16:0 37,12±0,88 28,26±2,28 18:0 4,88±0,64 2,76±0,04*

18:1 n9 25,23±0,34 12,22±1,72** 18:2n6 3,46±0,62 1,18±0,07* 18:3n6 0,07±0,017 1,87±0,09*** 22:0 0,28±0,07 0,89±0,07**

20:4n6+ 23:0 3,44±0,36 3,11±0,08 20:5n3 0,58±0,43 0,57±0,01 22:6n3 5,80±0,94 0,54±0,017***

: * <0,05; ** <0,01; *** <0,001 .

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Chebanov, M.; Rosenthal, H.; Gessner, J.; Van Anrooy, R.; Doukakis, P.; Pourkazemi, M.; Williot,P. Sturgeon hatchery practices and management for release–Guidelines FAO Fisheries and AquacultureTechnical Paper. No. 570. Ankara, FAO. 2011. 110pp. (http://www.fao.org/docrep/015/i2428e/i2428e.pdf).

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