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Lab 1: Chemistry, Pipetting, and Biuret’s TestDr. Sarah VanVickle-ChavezBIO 2960 LaboratorySpring 2013

+Chemistry for Biology Review

Use Firefox or Safari (Internet Explorer doesn’t always cooperate)

http://www.nslc.wustl.edu/courses/Bio2960/labs.html

There will not be a lab report due next week. But….

There are three reports due at the beginning of class in two weeks: 1a = proteins (we’ll do this next week) 1b = cell organelles (use your book/notes for this one) 2 = SDS-PAGE (we’ll do this next week)

+Pipetting

P-20 1-20 ± 0.5μl

P-200 20-200 ± 1 μl

P-1000 200-1000 ± 10 μl

The smaller pipettors are always more accurate.

The top number on the dial should be the top number in your volume.

Demo: http://www.youtube.com/watch?v=cxm61exlVnc

+Biuret’s Test

Additional Notes: http://www.youtube.com/watch?v=wtVGM6tIhHg http://www.piercenet.com/browse.cfm?fldID=876562B0-5056-8A76-4E0C-B764EAB3A339

Remember: Cu2+ = BLUE, but a positive rxn is VIOLET. Why?

Biuret’s = CuSO4 + NaOH (to keep pH basic)

If there’s a peptide bond, Cu2+ complexes w/ 4 Ns. The more bonds, the more complexes.