intromcb.berkeley.edu/courses/mcb141/2012/lecture/richard...mouse development (gilbert 6) wednesday,...

http://genex.hgu.mrc.ac.uk/Atlas/intro.html

Tuesday, March 30, 2010

Wednesday, April 4, 12

Human 128cell blastocyst before/during hatching

Mouse embryonic stem cells (ES cells)from the inner cell mass, growing in a culture dish

Injection of ES cells into theblastocyst cavity

“zona”

Holding pipetInjection pipet

Oct4 gene expression in epiblast


Lu, Brennan and Robertson (fig 1)


http://www.ncbi.nlm.nih.gov/pubmed/11448624




from Lu, Brennan and Robertson, 2001

Tuesday, March 30, 2010Wednesday, April 4, 12

Lu, Brennan and Robertson (fig 5)Gene expression demonstrates the emergence of pattern/breaking of symmetry








8

Mouse development (Gilbert 6)


http://www.ncbi.nlm.nih.gov/books/NBK10052/




Mouse turning (details are not on the exam)


10


11


12


Role of the AVE (anterior visceral endoderm) in establishing the anterior-posterior axis of the

mouse embryo

Cylindrical Symmetry Bilateral Symmetry

A P

Cerberus (anti-Nodal)Dkk (anti-Wnt)Lefty (anti-Nodal)

Otx2Anterior neural ectoderm

Nodal, Vg1,Cripto (co-receptorFor Nodal and Vg1)Endo-mesoderminduction

Day 5.25 Day 5.5 Day 6.25

orhypoblast

Endo-mesoderm induction,Primitive streakNodal, wnt, brachyury, fgf expressed

Epiblastectoderm


Mouse transgenesis (insertion of a foreign gene into the mouse genome)

Gene for red fluorescent protein (RFP)

Gene for green fluorescentprotein (GFP) from jellyfish

(e.g., GFPwith promoter)

FOSTERMOTHER

(2x)

(random)

non-homologous integration

Green ES cells

Dr. MarioCapecchi-1984Univ. of Utah


Gene knockoutby homologous recombination


Knockout of the lefty gene illustrates the need for a negative feedback on nodal expression

Mouse Lefty2 and Zebrafish Antivin Are Feedback Inhibitors of Nodal Signaling during Vertebrate Gastrulation

1 Chikara Meno1, 3, Kira Gritsman4, 3, Sachiko Ohishi2, Yasuhisa Ohfuji2, Elizabeth Heckscher4, Kyoko Mochida2, Akihiko Shimono3, 2, Hisato Kondoh3, William S Talbot4, Elizabeth J Robertson5, Alexander F Schier4, 1, Hiroshi Hamada1, 2, 1


http://mcb.berkeley.edu/labs/harland/links.html

http://mcb.berkeley.edu/labs/harland/links.html



intromcb.berkeley.edu/courses/mcb141/2012/lecture/richard...mouse development (gilbert 6) wednesday,...

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