بسم الله الرحمن الرحيم. primary ingredients required by all living organisms...
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140 micro
بسم الله الرحمن الرحيم
Lab 2: Culture Media
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Microorganisms need food to grow
Primary ingredients required by all living organisms include:
a carbon source, water, minerals, and
a nitrogen source. These nutrients together make a
media. Different microbes need different
amounts of these nutrients.
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Culture media may be found in one of three states:
liquid (called broth).
semi-solid. solid.
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Aim To prepare solid and Liquid
media
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Requirement
1) Deffrent media :Nutreint broth - Nutrient agar -
PDA/malt extract
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2- Balance 3- Distil water5- Test tubes 6- Petri plates7- Flaskes 7- Burners8- Autoclave
Requirement
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Procedure
1- Weigh
2- Dissolv
e3-
Sterilize
4- cool
5- refrigerate till
use
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1- Weigh
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2- Dissolv
e
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3- Steriliz
e
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4- cool
5- refrigerate till
use
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Who to make?.……
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Pouring of Solid media
: - Petri plates Remove the lid slightly Pour the media near bunsen burner Invert the plate Write date and time on the sides of plates
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After boiling, pour media in test tubes
Autoclave Place in slant position till the
media solidfys.
- Making of Slants:
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- Agar deep tube :
After boiling, pour media in test tubes
Autoclave Place in vertical position till the
media solidfys.
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Slant agar - Deep agar
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- Making of test tubes with broth media :
Place the testube near a burner and remove the cap
Pour the media in the tube and close the cap at once
Place the tube in upright position in the testube stand
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Thank you ….
الشهراني. شروق أ