© university of sydney australian cereal rust control program professor robert f. park...
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© University of Sydney© University of Sydney
AustralianCerealRustControlProgram
Professor Robert F. [email protected]
Australian Cereal Rust Control ProgramConsultative CommitteeDAFWA, Perth
March 21st 2013
Director’s Report
© University of Sydney© University of Sydney
WA residents told to prepare for Cyclone Rusty!
Monday 25th February 2013, AEST 9.00 am
http://www.abc.net.au/news/2013-02-25/wa-residents-told-to-prepare-for-cyclone-rusty/4537056
© University of Sydney
Scientific staff:Urmil Bansal
Harbans Bariana
Robert Park
Peng Zhang
Davinder Singh
Haydar Karaoglu
Peter Dracatos
Honorary Associates:William Cuddy
Bob McIntosh
Ray Hare
Colin Wellings
General staff:Muhammed Gill
Sami Hoxa
Keshab Kandel
Hanif Miah
Gary Standen
Matthew Williams
Personnel, as @ March 2013
Postgraduate students:Jordan Bailey
Mumta Chettri
Lida Derevnina
Banchgize Temesgen
Leila Kamino
Tolera Keno
Mandeep Randhawa
Huda Elmansour
Peace Kankwatsa
Vallence Nsabesyera
Mesfin Gesesse
Bosco Chemeyek
Ali Salman
© University of Sydney
Personnel changes during 2012
Four technical positions made redundant:
- Mr Gary Standen – Fee for Service, GRDC funded.- Mr Paul Kavanagh – Non-wheat rust surveys and genetics- Ms Kanwal Kaur – Greenhouse testing (FFS, Detailed tests)- Mr Keshab Kandel – Stripe rust surveys and genetics
Dr Wellings retired, June 2012
Park Appointment
New Appointments:
- Mr Gary Standen appointed, FFS (non-GRDC funds)- Mr Keshab Kandel appointed, Rust surveys- Dr Will Cuddy appointed, NSWDPI, Plant Pathologist Rust Diseases- Business Manager TBA-HEO 3 Technician TBA
© University of Sydney
Personnel changes during 2012
Four technical positions made redundant:
- Mr Gary Standen – Fee for Service, GRDC funded.- Mr Paul Kavanagh – Non-wheat rust surveys and genetics- Ms Kanwal Kaur – Greenhouse testing (FFS, Detailed tests)- Mr Keshab Kandel – Stripe rust surveys and genetics
Dr Wellings retired, June 2012
Park Appointment
New Appointments:
- Mr Gary Standen appointed, FFS (non-GRDC funds)- Mr Keshab Kandel appointed, Rust surveys- Dr Will Cuddy appointed, NSWDPI, Plant Pathologist Rust Diseases- Business Manager TBA-HEO 3 Technician TBA
© University of Sydney
Personnel changes during 2012
Four technical positions made redundant:
- Mr Gary Standen – Fee for Service, GRDC funded.- Mr Paul Kavanagh – Non-wheat rust surveys and genetics- Ms Kanwal Kaur – Greenhouse testing (FFS, Detailed tests)- Mr Keshab Kandel – Stripe rust surveys and genetics
Dr Wellings retired, June 2012
New Appointments:
- Mr Gary Standen appointed, FFS (non-GRDC funds)- Mr Keshab Kandel appointed, Rust surveys- Dr Will Cuddy appointed, NSWDPI, Plant Pathologist Rust Diseases- Business Manager TBA- HEO 3 Technician TBA
© University of Sydney
Previously, ACRCP was 1 project – now 4 projects:
1. ACRCP - National breeding support. US00064
2. ACRCP - Durable genes. US00063
3. ACRCP - Towards 2019 and a century of monitoring cereal rust pathogens in Australia. US00067
4. ACRCP- Molecular marker program CSIRO/Univ. Sydney/CIMMYT collaborative project. CSP00164
Current status of “new” projects [1 July 2012 thru 30 June 2017]
Proposals include:
- reducing Director’s salary to zero, incrementally, over project life- GRDC to fund (50%) Business Manager- Fee for Service subsidy to reduce to zero (wheat by year 3, barley by year 4)- full review of FFS, including costings, once Business Manager is appointed - establishment of a Breeder’s Advisory Group
© University of Sydney
Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007
Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade
The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc
Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….)
Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail
Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL.
Defence Trade Controls Act 2012
© University of Sydney
Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007
Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade
The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc
Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….)
Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail
Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL.
Defence Trade Controls Act 2012
© University of Sydney
Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007
Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade
The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc
Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….)
Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail
Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL.
Defence Trade Controls Act 2012
© University of Sydney
Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007
Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade
The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc
Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….)
Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail
Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL.
Defence Trade Controls Act 2012
© University of Sydney
Camden Campus - Centre for Carbon, Food & Water
© University of Sydney
Camden Campus – CER complex
© University of Sydney
PBI Infrastructure – greenhouse polycarbonate
© University of Sydney
Fee for Service, wheat test numbers 2007 through 2012
Wheat: Year
Test: 2007 2008 2009 2010 2011 2012
Field tests 121,082 80,642 100,374 96,811 60,338 45,076
Greenhouse tests 41,626 15,903 8,287 21,999 33,201 22,509
F2 Long Rows 217 295 277 944 611 199
Non-wheat: Year
Test: 2007 2008 2009 2010 2011 2012
Field tests 10,628 12,210 13,142 11,617 9,885 13,128
Greenhouse tests 12,518 13,726 15,021 17,327 24,993 30,128
© University of Sydney
Fee for Service, wheat test numbers 2007 through 2012
Wheat: Year
Test: 2007 2008 2009 2010 2011 2012
Field tests 121,082 80,642 100,374 96,811 60,338 45,076
Greenhouse tests 41,626 15,903 8,287 21,999 33,201 22,509
F2 Long Rows 217 295 277 944 611 199
Non-wheat: Year
Test: 2007 2008 2009 2010 2011 2012
Field tests 10,628 12,210 13,142 11,617 9,885 13,128
Greenhouse tests 12,518 13,726 15,021 17,327 24,993 30,128
© University of Sydney
Field rust inoculations in 2012- 19 pathotypes across 5 field sites
Crop Rust PathotypeWheat Leaf rust 104-1,2,3,(6),(7),11+Lr37 [=576] 104-1,2,3,(6),(7),11,13 [=547] 10-1,3,7,9,10,12 [=592] 76-1,3,5,7,9,10,12 +Lr37 [=621] Stem rust 98-1,2,3,5,6,7 [=580] 34-2,12,13 [=427] 34-1,2,7 +Sr38 [=565] 343-1,2,3,4,5,6 [=429] Stripe rust 134 E16 A+ Yr17+ Yr27+ [=617] 134 E16 A+ J+ T+ [=???] 150 E16 A+ [=598]Barley Leaf rust 5457P+ [=612] Stripe rust BGYROats Crown rust 4473-4,16,10 +BB [=567] 0107-4,6,10+WN [=595] 0207-1,5,6,10,12+G [=549] 0307-345610+WV [=605] 264 [=164] Stem rust 41+Pg9,13,a [=512]Triticale Stem rust 34-2,12,13 [=427] Stripe rust 134 E16 A+ J+ Yr27+ 134 E16 A+ J+ T+ [=???]
© University of Sydney
Field rust pathotypes – FFS sampling for pathotype ID
© University of Sydney
Field sites
Lansdowne 3 Year rotation, 4.5haHorseunit 3 Year rotation, 3.6haBreakwell 3 Year rotation, 1haKaralee 3 Year rotation, 0.7haJ.B. Pye* 3 Year rotation, 0.8haSheep Unit 3 Year rotation, 1.2ha
* Also have a further 7.5ha (2.5ha x 3 year rotation) available from 2013
© University of Sydney
Field – Karalee redevelopment
© University of Sydney
Dam
Roadway
“Cage 2” “Cage 1”
Tunnelhouses (horticulture)
Year 1
Year 2
Year3
X X
Field – Karalee redevelopment
© University of Sydney
Field - proposed restructure, Horse & Sheep Units
Horse Unit
Irrigation line
© University of Sydney
USyd ACRCP Outputs, 5.5 years, 01/07/2007 to 28/02/2013– Outreach and training
Wrote/ distributed/completed:
6 Annual Pathogenicity Survey reports
41 Cereal Rust Updates
75 extension articles for newspapers (incl. Ground Cover) etc
44 media releases including radio interviews.
Contributed to 43 grower/ agronomist workshops/ field days
Participated in 25 national/ international conferences/ meetings
Published 88 peer-reviewed journal articles/ book chapters
Completed 13 PhD and 5 Masters students, in rust pathology and genetics
© University of Sydney
Extension - All Stage / Adult Plant Resistance
© University of Sydney© University of Sydney
© University of Sydney
Genetic basis of disease responseRust resistance genotypes: Cereal Rust Reports
Rust Response Rust Resistance GenotypeLeaf Rust
Stem Rust
Stripe Eastern States
WA WA pt Yr17-27 pt Leaf Rust Stem RustStripe Rust
AGT Katana
MS MR MS-S MR-MS MR-MS Lr13Sr30
AGT Scythe
MS MS R-MR MS-S MS-S Sr13
Amarok R R S R MR-MS Lr14a, Lr37 Sr38 Yr17Annuello R-MR R-MR R-MR MS-S MS-S Lr24, Lr34 Sr24 Yr18Arrino MS MS S-VS S S Sr30 C
Arrivato MR MR R R-MR R-MR Sr8b, Sr9e, Sr13Axe MR MR MS R-MR R-MR Sr8bBabbler MS R R-MR MS-S MS-S Lr24, Lr34 Sr24 Yr18Banks MR MR MR MR-MS MR-MS Lr13 Sr30
Barham MR-MS MR-MS MS R MS-S Lr1, Lr20, Lr34, Lr37
Sr9b,Sr15, Sr38 Yr17, Yr18
© University of Sydney
© University of Sydney
Current research activities – Rust pathogens
DNA marker development – SSRs now available for:- P. graminis - all formae speciales- P. striiformis – all formae speciales- P. triticina- P. hordei
Old “104” “WA” pt. USA BGYR BYR Psd Psp
e.g. P. striiformis [Jordan Bailey]:
© University of Sydney
P
Stem rust race Ug99- lecture overview
Pgt sequencing initiative
- have 86 isolates to sequence- sequenced 61 so far, total cost $60k
- dendrogram based on 3.042 million polymorphic SNP loci
© University of Sydney
P
Stem rust race Ug99- lecture overview
Pgt sequencing initiative
- have 86 isolates to sequence- sequenced 61 so far, total cost $60k
- dendrogram based on 3.042 million polymorphic SNP loci
“Ug99” group
© University of Sydney
P
Stem rust race Ug99- lecture overview
Pgt sequencing initiative
- have 86 isolates to sequence- sequenced 61 so far, total cost $60k
- dendrogram based on 3.042 million polymorphic SNP loci
© University of Sydney
P
Stem rust race Ug99- lecture overview
Pgt sequencing initiative
- have 86 isolates to sequence- sequenced 61 so far, total cost $60k
- dendrogram based on 3.042 million polymorphic SNP loci
© University of Sydney
P
Stem rust race Ug99- lecture overview
Pgt sequencing initiative
- have 86 isolates to sequence- sequenced 61 so far, total cost $60k
- dendrogram based on 3.042 million polymorphic SNP loci
- informed selection of isolates for 1st cohort means a huge amount of information will be extracted from this data set
© University of Sydney
Wheat - APR and ASR to stripe rust, leaf rust and stem rust; cytogenetics
Barley - APR and ASR to leaf rust, ASR to stem rust; cytogenetics
Oats - APR and ASR to crown rust and stem rust
Triticale - APR and ASR to stripe rust, leaf rust and stem rust
Non-host / near-host resistance in cereals to rust diseases
Current research activities– Gene discovery & characterisation
© University of Sydney
Backcrossing stripe rust resistance genes into Avocet; 41 gene combination lines produced so far:
Current research activities– Gene discovery & characterisation
Yr1/Yr6Yr1/Yr9Yr1/Yr10Yr1/Yr17Yr1/Yr26Yr1/Yr27Yr1/Yr32Yr1/YrSp
Yr5/Yr26Yr5/Yr27
Yr6/Yr27Yr6/Yr9Yr6/Yr17Yr6/Yr24Yr6/Yr32Yr6/YrSp
Yr7/Yr17Yr7/Yr24Yr7/Yr27Yr7/Yr32
Yr10/Yr15Yr10/Yr24Yr10/Yr26Yr10/Yr27
Yr15/Yr26Yr15/Yr24//Yr10
Yr17/Yr27Yr17/Yr32
© University of Sydney
The Rust Bust Campaign
Belinda’s Rust Bust Presentation
© University of Sydney© University of Sydney