012015 lami resutls ppt

Fig 1 Axial CT and MRI images of lumbar spine on postoperative day 1 from rats before laminectomy (A) or on postoperative day 1 (B) or rats that underwent laminectomy (C) or sham surgery (D). Dorsal nerve roots are not damaged after laminectomy. There is extensive posterior inflammation and edema in the soft tissues at the site of incision.

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Fig 2 Effect of laminectomy on the voluntary wheel running.Time course of the effect in the distance travelled overnight (A), acceleration (B), maximum speed (C) induced by laminectomy (●), in comparison to sham operated rats(○).Statistically significant differences between the baseline and the values obtained after laminectomy:# P<0.05; ## P<0.01,### P<0.001 (one way ANOVA followed by Dunnett’ s test)and between the postoperative rats and sham operated rats in the same day of evaluation: * P<0.05; ** P<0.01, *** P<0.001,**** P<0.0001 (Two-way ANOVA followed by Tukey post hoc test). Each point and vertical line represents the mean± SEM of obtained values (n=8-10 per group).

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Laminectomy ShamBaseline 100±0 100±0POD0 6.32±1.99 88.49±17.16POD1 48.36±6.84 114.11±18.63POD2 60.68±10.46 96.73±12.66POD3 86.54±10.03 93.51±10.97POD4 98.42±7.59 91.28±13.13POD5 102.78±14.80 106.64±14.68POD6 100.49±15.35 99.04±15.52POD7 106.03±14.42 100.73±2.89

Wheel running acceleration



Wheel running maximum speed

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Fig 3 Effect of laminectomy on the nighttime total motor movement and rearing activities.Time course of the effect in the total motor movement(A) and rearing (B) induced by laminectomy (●), in comparison to sham operated rats(○).Statistically significant differences between the baseline and the values obtained after laminectomy:# P<0.05; ### P<0.001 and between the postoperative rats and sham operated rats in the same day of evaluation: * P<0.05; ** P<0.01, ****P<0.0001 (Two-way ANOVA followed by Tukey post hoc test). Each point and vertical line represents the mean± SEM of obtained values (n=8-10 per group).

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Laser fine motor distance Laser rearing

Laminectomy ShamBaseline 100±0 100±0POD0 61.10±4.53 89±2.95POD1 75.72±2.66 99.08±3.33POD2 88.54±3.43 99.83±4.00POD3 97.62±2.85 104.08±2.88POD4 100.19±3.16 104±3.30POD5 99.19±2.91 105.42±3.19POD6 102.78±3.33 102.6±4.74POD7 107.29±5.74 97.75±5.01


Fig 4 Effect of local administration of bupivacaine and ketoprofen on overnight voluntary distance running (A), acceleration (B), and maximum running speed (C) after laminectomy. Each bar and vertical line represents the mean ± SEM of the percentages relative to baseline values obtained in sham rats and postoperative rats 24 hours after surgery (n = 8–10 per group). Rats received continuous intramuscular injection of 1mg/kg/hr bupivacaine or 0.2mg/kg /hr ketoprofen. There were statistically significant differences between the saline group (white bar), and the experimental groups(grey bars) (unpaired t test) :**P < 0.01,***P<0.001.

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Fig 5 Effect of RTX on overnight voluntary distance running (A), acceleration(B), maximum running speed (C) of postoperative rats. Each bar and vertical line represents the mean ± SEM of the percentage relative to basal value, obtained in vehicle group and RTX group 24 hours after surgery (n = 8–12 per group). Rats received intrathecal injection of 200ng RTX 7 days before laminectomy. There were statistically significant differences between the saline group (white bar), and the RTX group (grey bar):*P < 0.05 (unpaired t test).

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Fig 6 Effect of RTX on total motor movement (A), and rearing (B) of postoperative rats. Each bar and vertical line represents the mean ± SEM of the percentage relative to basal value, obtained in vehicle group and RTX group 24 hours after surgery (n = 8–12 per group). Rats received intrathecal injection of 200ng RTX 7 days before laminectomy. There were statistically significant differences between the saline group (white bar), and the RTX group (grey bar):*P < 0.05,**P<0.01 (unpaired t test).

Fig 6 Time spent in one conditioned place preference chamber in post laminectomy rats treated with 10μg of morphine given intrathecally. Each bar and vertical line represents the mean± SEM of values obtained in preconditioning and postconditioning periods (n=15 per group). Statistical significant differences between the values : * P<0.05 (Student’s t test).

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012015 lami resutls ppt

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