103/11/2015

Opportunities and challenges of DDGSThe role of biotechnology

Budapest, the 5th November 2015Anne-Marie Debicki-Garnier, Technical Director EMEA, DuPont IB

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Main challenges in poultry and swine sectors

Raw material price versatility/need for flexibility

Cost of the feed/cost of the meat

Environment

Health status

Welfare

…

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Issues animal producers are facing today

Wheat Barley Corn Oats Wheat bran

Rye50

60

70

80

90

100

Variation in cereal prices relative to wheat (wheat = 100)

Pric

e v

aria

tion

(%

)

Soybean meal Corn/wheat DDGS

Rapeseed meal Sunflower meal40

50

60

70

80

90

100

Variation in prices for protein rich raw materials (soybean meal = 100)

Pric

e v

aria

tion

(%

)

Calculated form raw material prices June 2014, source: feedinfo.com

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Issues animal producers are facing today

Soybean meal Corn/wheat DDGS

Rapeseed meal Sunflower meal40

50

60

70

80

90

100

Variation in prices for protein rich raw materials (soybean meal = 100)

Pric

e v

aria

tion

(%

)→ a reduction in price is often correlated with an increase in fibre content of the raw material

Soybean meal Wheat DDGS Corn DDGS Rapeseed meal

Sunflower meal0

5

10

15

20

25

30

Variation in crude fibre content (%) between different protein rich raw materials

Cru

de

fib

re c

on

ten

t (%

)

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Issues animal producers are facing today

Fibre and environmental pressure

• ↑ nutrient excretionAs fibre reduces nutrient digestibility and increases endogenous

losses more nutrients are excreted→environmental concern →cost

• ↑ manure productionIncreasing fibre linearly increases amount of daily fecal-matter

excretion, due to water-binding capacity resulting in increased bulk

• ↑ animal welfare Fibre results in more satiety, which leads to more quite animals

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Fibre and fibre degrading enzymes

Aleurone cells

Endosperm

Embryo

Pericarp

Starch granules

Storage protein

Dietary fibre consist of non-starch polysaccharides (NSP) such as cellulose and arabinoxylan (AX)

AX is a copolymer of two pentose sugars – arabinose and xylose

AX is the substrate for xylanases

O

OHOH

OOH

OHO

O

OOH O

OHOH

OOH O

OH

OOH O

O

OO O

OHOH

OOH O

OHOH

OOH O

OH

OH

OOH

OH

OH

OO

OH

O2+

OOH

OH

OH

OOH

OH

OH

OH

OCH3

O

Arabinoxylan (AX) is found in both the endosperm of the cereal and in the pericarp

g/100g dry matter

Corn DDGS*

Fat (AH)

Crude protein

Crude fibre

NDF

ADF

Total NSP

Cellulose

NCP

Xylose

Arabinose

Uronic acid

A:X ratio

UA:X ratio

10,2

30,4

9,5

38,7

11,8

32,8 (2,9)

9,1

9,4 (0,5)

7,0 (0,7)

1,8 (0,5)

0,74

0,19

* Pedersen et al, 201503/11/2015

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Xylanase and Protease have demonstrated increased solubilisation of pentosans (AXOS) and protein in corn DDGS

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* Pedersen et al, 2015

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How Xylanase, Protease and Phytase improve nutrient retention

Aleurone layer cells

Aleurone layer contains NSP, protein and phytic acid which reduce substrate accessibility to digestive enzymes

Exogenous Xylanases degrade fibre and release nutrients Addition of protease increases the solubilization of protein and of pentosan,

indicating a close interaction of protein and AX in the cell wall Access of phytase to phytate requires breakdown of the aleurone layer

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Does higher solubilization of fibre in-vitro translate into better

nutrient digestibility and performance in-vivo?

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IN BROILERSPERFORMANCE – THE XAP CONCEPT

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1103/11/2015

3378a

3352ab

3286b

3344ab

Corn control Corn Axtra XAP Mixed control Mixed Axtra XAP

Bodyweight Gain (1-42 days; g)

ab Values without a common superscript are significantly different (P<0.05);• Mixed diets contained 10 and 14% corn-DDGS in starter and finisher respectively and 5% canola meal

Effect of XAP combination on performance of broilers to 42d

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1.60ab

1.57bc

1.63a

1.54c

Corn control Corn Axtra XAP Mixed control Mixed Axtra XAP

FCRc* (1-42 days)

-1.9% -5.5%

abc Values without a common superscript are significantly different (P<0.05);• FCR corrected 3 points for every 100g difference in bodyweight versus the corresponding positive control• Mixed diets contained 10 and 14% corn-DDGS in starter and finisher respectively and 5% canola meal

XAP combination reduced FCRc, particularly on mixed diets

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IN BROILERSDIGESTIBILITY – XAP AND PHYTASE

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XAP.NZ.B.29, Massey University, New Zealand

ab values without a common subscript are significantly different (P<0.05)

3264c

3369bc 3387ab

3488a

Control Control+1000FTU Axtra PHY

Control+ Axtra XAP

Control+1000FTU Axtra PHY

+Axtra XAP

Ileal DE(kcal/kg DM, 35-42 days)

+105 kcal

+ 224 kcal

+ 101kcal

XAP and Buttiauxella phytase improve nutrient digestibility of broilers fed on a corn/soybean meal/corn DDGS based diet

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XAP.NZ.B.29, Massey University, New Zealand

* denotes values which are significantly different from the unsupplemented control diet (P>0.05)

3.3 2.5

*5.5

*7.1 *

4.9*

4.43.1

*3.5

*7.1

*9.0

*6.0 *

4.8*

4.6

*6.6

*9.0

*15

*8.4

*7.6

Lysiene Methionine Threonine Cysteine Valine Total amino acids

Amino acid digestbility (% of control, 35-42 days)

Control + 1000 FTU/kg Axtra PHY Control + Axtra XAP Control + 1000 FTU/kg Axtra PHY +Axtra XAP

XAP and Buttiauxella phytase improve nutrient digestibility of broilers fed on a corn/soybean meal/corn DDGS based diet

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IN FATTENING PIGSGROWER – DIGESTIBILITYXYLANASE

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(ab) Values without a common superscript are significantly different (P<0.1)

3011(b)

3194(a)

Control Danisco Xylanase (2000U)

Ileal digestible energy (kcal/kg DM)

+183kcal (6.1%)

Effect of Xylanase on nutrient digestibility in grower pigs fed diets with 40% corn DDGS

03/11/2015 DX.US.S.37, University of Illinois, USA

18DX.US.S.37, University of Illinois, USA

38.5(b)

46.5(a)

Control Danisco Xylanase (2000U)

Ileal NDF digestibility (%)

+21%

(ab) Values without a common superscript are significantly different (P<0.1)

Effect of Xylanase on nutrient digestibility in grower pigs fed diets with 40% corn DDGS

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IN FATTENING PIGSFINISHER – PERFORMANCEXYLANASE AND PHYTASE

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-9.2%

FCR

2.86ab

3.05a

2.86ab

2.77b

abP<0.05

Positive Control Negative Control + Phyzyme XP

Negative Control +Phyzyme XP

+ Xylanase Danisco

Negative Control

Effect of Phytase and Xylanase on performance of pigs fed corn DDGS – 61 to 123 Kg body weight

03/11/2015 Reference: Phyzyme XP.S.USA.09.22 9300.USA.09.37, University of Kentucky, USA

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HOW TO MAXIMIZE VALUE ?THE SYNCRA CONCEPT

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XAP/phytase Enzymes

• Reduce undigested nutrients (Starch + protein)

• Reduced microbial growth or population

Develop healthy gut with diverse microbial populations by:

DFMs

• Secrete bacteriocins effective at inhibiting APEC and C. Perfringes

To provide maximum value we need a multi-factorial approach. Each component contributes to deliver an effective solution for gut health

Seeding the Gut, reduce pathogen colonisation

•Maintain microbial diversity

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2303/11/2015

3659a

3579b

3704a

PC NC NC + XAP + Enviva Pro

Bodyweight gain(g, 1-42 days)

ab Values without a common superscript are significantly different (P<0.05)

Enzyme and DFM in combination improve performance of broilers fed corn/soy/DDGS based diets reduced in energy and amino acids

XAP.NZ.B.12.28+EnvivaPRO.NZ.B.12.09, Massey University

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100

103

98.2

Positive Control (PC) Negative Control (NC) NC+ XAP + Enviva Pro

Relative cost per kg liveweigt gain (%)+

+ Calculation accounts for product cost

Enzyme and DFM in combination exhibited the lowest relative cost per kg liveweight gain in broilers fed corn/soy/DDGS based diets

XAP.NZ.B.12.28+EnvivaPRO.NZ.B.12.09, Massey University

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Take Home Messages

Net effects of enzymes on performance are greater in diets with greater undigested substrates, which normally correspond to diets with greater fibrous ingredients eg corn DDGS

Need for good knowledge of mode of action and robust matrices to work with enzymes combinations

Addition of relevant enzyme combination reduces feed cost whilst at least maintaining performance

Optimal value needs a multi-factorial approach – e.g. the Syncra concept

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Thanks very much for your attention

Questions?

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03/11/2015