04 anammox process for nitrogen removal - a review
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ANAEROBIC AMMONIUM OXIDATION(ANAMMOX) PROCESS FOR NITROGEN
REMOVAL - A REVIEW
ARTICLE
READS
176
2 AUTHORS:
Obuli Karthik
Hong Kong Baptist University
47 PUBLICATIONS 232 CITATIONS
SEE PROFILE
Kurian Joseph
Anna University, Chennai
82 PUBLICATIONS 584 CITATIONS
SEE PROFILE
Available from: Kurian Joseph
Retrieved on: 05 March 2016
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ANAEROBIC AMMONIUM OXIDATION (ANAMMOX) PROCESS FOR
NITROGEN REMOVAL – A REVIEW
Obuli P. Karthikeyan and Kurian Joseph
Centre for Environmental Studies, Anna University, Chennai – 600 025.
Tamil Nadu, INDIA
ABSTRACT
In wastewater treatment, nitrogen is being considered a one of the essential parameter as
it has significant adverse impacts on the environment. Anaerobic ammonia oxidation
( ANAMMOX ) is a novel process in which nitrite is used as the electron acceptor in the
conversion of ammonium to nitrogen gas. The ANAMMOX process offers great opportunities to
remove ammonia in fully autotrophic systems with biomass retention. No organic carbon is
needed in such nitrogen removal systems, since ammonia is used as electron donor for nitrite
reduction. This paper reviews and summarizes recent developments in nitrogen removal,
microbial aspects (occurrence, physiology, microbiology and biochemistry) of ANAMMOX,
followed by a qualitative comparison of several components of ANAMMOX technology with
conventional nitrogen removal systems and finally addresses the application of the ANAMMOX
process for nitrogen management.
KEYWORDS Wastewater, nitrogen, nitrification, and ANAMMOX
------------------------------------------------------------------------------------------------------------------------------------------------------------------
* Research Scholar and Corresponding author: [email protected]
1 Assistant Porfessor: [email protected]
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INTRODUCTION
An increasing population and industrialization will increase our water demand, placing
even more pressure on water resources. Conventional wastewater treatment plants have not been
designed for nitrogen removal, and many plants do not meet the current discharge limits (Jetten
et al., 2002). Wastewater originated from many other sources such as tannery, food processing,fertilizer manufacturing, slaughter house and landfill leachate contain greater amount of nitrogen
load, which should be treated before discharge into the surface water body (Table 1). Wastewater
containing huge amount of nitrogen compounds is not allowed to be released to the surface wateras it has ecological impacts and can affect human health (Kelter et al. 1997).
Chemical, Physicochemical and biological methods are broadly used for treatment ofwastewater loaded with highly concentrated NH4
+-N. In considering the criterion like cost-
benefit analysis, requirement of energy and chemical doses, familiarity with operational
procedures, and environmental sustainability, a particular treatment for a specific pollutant is
usually selected (Mulder et al 2003). Still, the tradition is that depending on the concentration of
nitrogen load presenting in the collected wastewater specimen, either physicochemical or biological treatment method is decided. According to Mulder (2003) three concentration ranges
could be differentiated:
• NH 4+-N concentration less than 100 mg NH4+-N/l - In this range biological N-removal is
the preferred process based on cost-effectiveness. Domestic wastewater is within this
range.
• NH 4+-N concentrations in the range 100-5000 mg NH 4
+-N/l - A typical example is sludge
liquor for which after extensive investigations biological treatment was preferred (Janus
et al., 1997). Although ammonia stripping and producing MgNH4PO4 were identified as
interesting alternatives for resource recovery these options were not cost-effective
(Priestley et al., 1995; Janus et al., 1997).
•
NH 4+
-N concentrations greater than 5000 mg NH 4+
-N/l - In this range physicochemicalmethod are technically and economically feasible. A successful example is the steamstripping of a wastewater with an ammonium concentration of 1.5% followed by
ammonia recovery which has been in operation on industrial scale since 1985 (Harmsenet al., 1986).
Table 1. Wastewater containing high concentrations of nitrogen content
Sources of Nitrogen Total Nitrogen (mg/L) Reference
Landfill leachate 500 - 2500 Chung et al (2003)
Starch Production 800 - 1100 Abeling and Seyfried (1993)
Wastewater from pectin
industry
1600 Deng Peterson et al (2003)
Wastewater from slaughter
house, after treatment in
aerobic lagoon
170 - 200 Keller et al (1997)
Wastewater from tannery 128 - 185 Marat et al (2003)
Discarded water 260 - 958 Gil and Choi (2004)
Source; Hulle 2005
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BIOLOGICAL NIROGEN REMOVAL
The biological process is cheaper and is the most widely practiced approach for nitrogen
control in wastewater treatment. Conventional biological nitrification and denitrification
processes have received the maximum attention. In Table 2, three process options of the new
system are presented and compared to a conventional nitrogen removal system based onautotrophic nitrification and heterotrophic denitrification system.
AEROBIC AND ANAEROBIC AMMONIUM OXIDATION
Ammonium oxidation has been observed in many bacterial species. Ammonia is oxidized by two pathways: first, ammonia is oxidized to nitrite by hydroxylamine, which is then oxidized
to nitrate by hydroxylamine oxidoreduxctase; Second, ammonia and nitrite are anaerobically
converted to nitrogen gas. The aerobic chemolithoautotrophic ammonia oxidizing bacteria
(AOB) are specialists that can grow on ammonia and carbon dioxide (Purkhold et al., 2000) anduse ammonia monooxygenase to convert ammonia into hydroxylamine. Many heterotrophic
bacteria, such as P. Pantotropha and Alcaligenes faecalis strain TUD (Otte et al., 1999), can
carry out the same reaction. Methanotrophs are capable of converting ammonia tohydroxylamine via the methane monooxygenase, whereas the ammonium monooxygenase can
oxidize methane to carbon dioxide. The recently identified lithotrophic planctomycete possessesthe ANAMMOX pathway, which is coupled to nitrite reduction (Strous et al., 1999).
Table 2. Qualitative comparison of several components of the ANAMMOX technology with
conventional nitrogen removal systems
Nitrifiers+ Various
heterotrophs
Aerobic NH4+oxidizers
+ Planctomycetes
Planctomycetes Aerobic NH4+
Oxidizers
Bacteria
0.05-41-36-121Reactor capacity (kg
N/m3day)
HighLowLowLowSludge production
YesNoneNoneNoneCOD requirement
NoneYesYesNoneBiomass retention
YesNoneNoneNonepH control
HighLowNoneLowOxygen requirements
Oxic; anoxicOxygen limited AnoxicOxicConditions
N2, NO3- ; NO2
-N2, NO3-N2, NO3
-NH4+, NO2
-Discharge
Wastewater Wastewater Ammonium nitrite mixtureWastewater Feed
2111Number of reactor
Conventional
nitrification,
denitrification
CANANON ANAMMOXSHARONSystem
Nitrifiers+ Various
heterotrophs
Aerobic NH4+oxidizers
+ Planctomycetes
Planctomycetes Aerobic NH4+
Oxidizers
Bacteria
0.05-41-36-121Reactor capacity (kg
N/m3day)
HighLowLowLowSludge production
YesNoneNoneNoneCOD requirement
NoneYesYesNoneBiomass retention
YesNoneNoneNonepH control
HighLowNoneLowOxygen requirements
Oxic; anoxicOxygen limited AnoxicOxicConditions
N2, NO3- ; NO2
-N2, NO3-N2, NO3
-NH4+, NO2
-Discharge
Wastewater Wastewater Ammonium nitrite mixtureWastewater Feed
2111Number of reactor
Conventional
nitrification,
denitrification
CANANON ANAMMOXSHARONSystem
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The ANaerobic AMMonium OXidation ( ANAMMOX ) process, which was discovered
10 years ago (Mulder, 1992) but already predicted to exist 30 years ago (Broda, 1977), couldoffer an alternative for the treatment of this return stream. Later, Van de Graff et al. (1997) and
Bock et al. (1995) observed that nitrite was the preferred electron acceptor for the process. Also,
other streams with high nitrogen and low carbon content such as landfill leachates and
evaporator condensates could be treated. In the ANAMMOX process ammonium is oxidizedunder anoxic, i.e. oxygen depleted, conditions with nitrite as electron acceptor. Ammonium and
nitrite are consumed on an almost equimolar basis. The ANAMMOX process should always be
combined with a partial nitritation process, such as the SHARON process (van Dongen et al.,2001a&b), where half of the ammonium is oxidized to nitrite. Both autotrophic processes will
increase the sustainability of wastewater treatment as the need for carbon addition (and
concomitant increased sludge production) is omitted and oxygen consumption and the emissionof nitrous oxide during oxidation of ammonia are largely reduced (Jetten et al., 1997). As such,
the combined process (partial nitritation and ANAMMOX ) was termed autotrophic nitrogen
removal process (Jetten et al., 2002).
OCCURANCE
The existence of ANAMMOX bacteria capable of producing nitrogen gas from ammonium
and nitrate/nitrite was demonstrated for the first time in denitrifying fluidized bed reactor treating
sewage sludge digester effluent and ammonia laden wastewater (Mulder et al., 1995; and Van deGraff et al., 1995), in marine sediments (Thamdrup and Dalsgaard, 2002), Black sea sediments
N2
TA
TNO
+ O2
+ O2
NO3-
+ COD
+ COD
TNO
N2
TA
TNO
ANAMMOX + O2
Nitrogen Fixation
Classical nitrogen removal
Autotrophic nitrogen removal
Figure 1. ANAMMOX process in nitrogen cycle
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(Kuypers et al., 2003), anoxic waters of Golf Dulce, a 200 m deep coastal bay in Costa Rica
(Dalsgaard et al., 2003) and landfill environments (Chung et al., 2003).
MICROBIOLOGY OF ANAMMOX
Microbial nitrogen metabolism also plays an important role in the global nitrogen cycle.
Microbial activities, such as denitrification and ANAMMOX , are the major mechanisms that
convert combined nitrogen to dinitrogen gas, thereby completing the nitrogen cycle. The updatednitrogen cycle with ANAMMOX is depicted in Figure 3 (after Jetten et al., 1999). Nitrification is
the aerobic oxidation of NH3 to NO3-. It consists of two sequential steps carried out by two
phylogenetically unrelated groups of aerobic chemolithoautotrophic bacteria. Some heterotrophic bacteria can also oxidize ammonium to nitrate, but this is only a very small contribution to the
overall ammonia oxidation (Pynaert, 2003). No single known autotrophic bacterium is capable of
complete oxidation of NH3 to NO3- in a single step (Abeliovich, 1992). In view of coupling a
partial nitrification unit with an Anammox unit, nitrite oxidising activity should be suppressed
and TAN should only be oxidised for about 50 % to TNO2.
The physiology of anaerobic ammonium oxidizing aggregates cultivated in a sequencing batch reactor was investigated by Strous et al. (1999). The maximum specific substrate
conversion rate of the ANAMMOX biomass was measured as a function of temperature and pH in
batch experiments. From the temperature dependency of ANAMMOX activity, the activationenergy was calculated to be 70 kJ/mol. Strous et al. (1998) have also reported that the affinity
constants for the substrates, ammonium and nitrite, are less than 0.1 mg N/L inhibited
ANAMMOX process completely. In another study Strous et al. (1999) have shown that the ANAMMOX process was reversibly inhibited by the presence of oxygen.
Bacteria capable of anaerobically oxidizing ammonium had not been known earlier and
were referred as the “lithotrophs missing from nature” (Shivaraman and Geetha, 2003). These
missing lithotrophs were discovered and identified as the new autotrophic members of the order
of planctomycete, one of the major distinct division of bacteria (Strous et al., 1999a). Theanaerobic ammonium oxidation reaction is carried out by two ANAMMOX bacteria that have
been tentatively named as “Brocardia anammoxidans” (Strous et al., 1999a) and “Kuenenia
stuttgartiensis” (Schmid et al., 2000). The high ANAMMOX activity observed for both bacteriain a pH range between 6.4 and 8.3 and temperature between 20
oC and 43
oC (Strous et al., 1999b;
and Egli et al., 2001). The ANAMMOX bacterial activity is 25-fold higher than aerobic nitirifying
bacterial oxidation of ammonium under anoxic conditions when using nitrite as the electronacceptor (Jetten et al., 1999). Acetylene, phosphate and oxygen are known to be strongly
inhibiting ANAMMOX activity (Van De Graaf et al., 1996).
BIOCHEMISTRY OF ANAMMOX
The possible metabolic pathways for anaerobic ammonium oxidation are depicted in
Figure 4. (Van de Graff et al., 1997). The ANAMMOX process is based on energy conservationfrom anaerobic ammonium oxidation with nitrite as electron accpetor without addition of
external carbon source (Jetten et al., 1999). Hydrazine and hydroxylamine are known to be some
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ANAMMOX reaction establishes a proton gradient by the effective consumption of protons in the
riboplasm and production of protons inside the anammoxosome, a mechanism known asseparation of charges. This result in an electrochemical proton gradient directed from the
anammoxosome to the riboplasm. Based on isotopic carbon analysis Schouten et al. (2004)
concluded that different ANAMMOX bacteria, such as Candidatus Scalindua sorokinii and
Candidatus Brocadia anammoxidans use identical carbon fixation pathways, which may beeither the Calvin cycle or the acetyl coenzyme A pathway.
APPLICATION OF ANAMMOX IN LEACHATE TREATMENT
Recent research has permitted the development of new ways of nitrogen removal, such as
the partial nitrification and the anaerobic oxidation of the ammonium ( ANAMMOX ), whichrepresent significant advances in the field of biological removal of the nitrogen pollution. The
application of a combined partial nitrification– ANAMMOX process to the treatment of high
ammonia nitrogen content influents, ex. leachate, is particularly promising. It would lead to
potential savings of up to 60% in oxygen generation and 100% in external carbon, besides
significantly reducing the sludge generation and the net emission of CO2 (Van Dongen etal.,2001), diminishing the total treatment operating cost up to 90 % (Jetten et al., 2001). The
introduction of partial nitrification/ ANAMMOX to the treatment of high-strength wastewaterswill lead to substantial savings of energy and resources. Such systems have been tested over
prolonged periods and demonstrated stable effluent quality and compact ammonium removal
without the need for process control. Given the low costs of our system, a full-scaleimplementation is to be expected in the near future.
LIMITATIONS
ANAMMOX coupled to nitrite reduction offers opportunities in the area of processdevelopment of nitrogen removal systems. One of the biggest challenges is how to accelerate the
slow rate of nitrogen removal from these systems (the rate is less than half that of aerobic
nitrification) (Strous et al., 1999; and Jetten et al., 1998). However, from a commercial
application perspective, the more challenging issue is the extremely slow growth rate (10-14days) of the bacteria known to carry out these reactions. Similar to aerobic nitrification,
ANAMMOX is subjected to inhibition. This process requires anaerobic conditions for ammonia
oxidation, but inhibition by oxygen is reversible
FUTURE STUDY
ANAMMOX technology has been evaluated using synthetic wastewater/sludge digester
effluent from domestic WWTP. Research is necessary to know the feasibility of applying
ANAMMOX process technology with other actual wastewater and leachates using appropriatereactor types and configuration. The performance of ANAMMOX process in treating actual
wastewater/leachate would not only depend on ANAMMOX bacteria but also on the co-existence
of other important oxygen scavenging and ammonia generating/ammonia to nitrite oxidizing
bacteria. Research is needs to be carried out to work out optimal conditions for such anecosystem to sustain in a reactor and develop methodologies to monitor the responsible
microbial community in the system. Applied genomic research can be used to identify genes and
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patterns of expression that are critical to the performance of nitrogen metabolism in responses
can be coupled with reporter systems for the development of online measurement systems.Coupling the advances related to bacterial nitrogen metabolism with improved monitors of
macroscopic performance should lead to more robust operating strategies for wastewater
bioreactors. Genomic information, in combination with traditional biochemical, genetic and
ecological studies is needed to understand the inorganic nitrogen metabolism, and thus benefittheir industrial applications.
ACKNOWLEDGEMENT
The authors wish to thank the financial support from Swedish International Development
Agency (SIDA), and technical co-ordination from Asian Institute of Technology (AIT),Bangkok, Thailand. The cooperation of Chennai Corporation in sample collection from
Kodungaiyur and Perungudi dumping grounds is gratefully acknowledged.
REFERNECES
Abeliovich, A., “Transformations of ammonia and the environmental impact of nitrifying bacteria,” Biodegradation, Vol. 3, 255-264 (1992).
Broda E., “Two kinds of lithotrophs missing in nature,” Z.Allg. Mikrobiol, Vol.17, Pp. 491-493
(1977).
Egli K., Fanger U., Alvarez P.J.J., Siegrist H., Van der Meer J.R. & Zehnder A.J.B.,“Enrichment and characterization of an anammox bacterium from a rotating biological
contactor treating ammonium-rich leachate,” Archive of Microbiology., vol. 175, 198-207
(2001).
Harmsen, L.W.F., Lourens, P.A. and Leeuwen, H.J.M.L., “Stikstofverbindingen verwijderen uit
afvalwater,” P.T./Procestechniek 41, 27–29 (1986).
Hulle, V. S., “Modelling Simulation and optimization of autotrophic nitrogen removal proceses,”Doctoral Thesis in Environmental Technology, Applied Biological Sciences, University of
Gent, Belgium (2005).
Janus, H.M. and Vander, R.H.F., “Don’t reject the idea of treating reject water,” Water Scienceand Technology, 34 (3-4), pp 87-94 (1997).
Jetten M.S.K., Schmid M., Schmidt I., Wubben M., Dongen U.V., Abma W., Sliekers O.,
Revsbech N.P., Beaumont H.J.E, Ottosen L., Volcke E., Laanbroek H.J., Campos-Gomez
L.J., Cole J., Loosdrecht M.V., Mulder J.M., Fuerst J., Richardson D., Katinka van de Pas,Mendez-Pampin R., Third K., Cirpus I., Spanning R.V., Bollmann A., Nielsen L.P., Camp
H.O., Schultz C., Gundersen J., Vanrolleghem P., Strous M., Wagner M., and Kuenen J.G.,“Improved nitrogen removal by application of new nitrogen-cycle bacteria,” Reviews inEnvironmental science and biotechnology, Vol. 1, 51-63 (2002).
Jetten, M.S.M., Strous, M., van de Pas-Schoonen, K.T., Schalk, J., van Dongen, U.G.J.M., Van
De Graaf, A.A., Logemann, S., Muyzer, G., van Loosdrecht, M.C.M. and Kuenen, J.G., “The
anaerobic oxidation of ammonium,” FEMS Microbiology Reviews, Vol. 22, 421- 437(1999).
IN -109
-
8/17/2019 04 ANAMMOX Process for Nitrogen Removal - a Review
10/11
Jetten, M.S.M., Wagner, M., Fuerst, J., van Loosdrecht, M., Kuenen, G., and Strous, M.,
“Microbiology and application of the anaerobic ammonium oxidation (‘anammox’) process,”Current Opinion in Biotechnology, Vol. 12, 283–288 (2001).
Kelter, P. B., Grundman, J., Hage, D. S., Carr, J. D., “A Discussion of water Pollution in the
United States and Mexico: with High School Laboratory Activities for Analysis of Lead,
Atrazine, and Nitrate,” J. of Chemical Education. 74 (12), 1413-1421 (1997).Kuenen, J.G. and Jetten, M.S.M., “Extrao rdinary anaeribic ammonia-oxidizing bacteria,” ASTM
News, Vol. 67, 456-463 (2001).
Mulder A., Van de Graff A.A., Robertson L.A. & Kuenen J.G., “Anaerobic ammonium
oxidation discovered in a denitrifying fluidized bed reactor,” FEMS Microb. Ecol., Vol. 16,177-184 (1995).
Mulder, A., “The quest for sustainable nitrogen removal technologies,” Water Science and
Technology, 48 (1), 67-75 (2003).
Otte S., Schalk J., Kuenen J.G. and Jetten M.S., “Hydroxylamine oxidation and subsequentnitrous oxide production by the heterotrophic ammonia oxidizer Alcaligenes faecalis,”
Applied Microbiology and Biotechnology, Vol. 51, 255–261 (1999).Priestley, A.J., Cooney, E., Booker, N.A. and Fraser, I., “Nutrients in wastewaters-ecological
problem or commercial opportunity?,” In: Proceedings 17Th AWWA Federal convention.Melbourne, Australia, March, 16-21, 1997, 340-346 (1997).
Purkhold U., Pommerening-Roser A., Juretschko S., Schmid M.C., Koops H.P. and Wagner M.,
“Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNAand amoA sequence analysis: implications for molecular diversity surveys,” Applied
Environmental Microbiology, Vol. 66, 5368–5382 (2000).
Pynaert, K., Smets, B.F., Wyffels, S., Beheydt, D., Siciliano, S.D., and Verstraete, W.,
“Characterization of an Autotrophic Nitrogen-Removing Biofilm from a Highly Loaded Lab-Scale Rotating Biological Contactor,” Appl. Environ. Microbiol., June, 3626–3635 (2003).
Schalk, J., De Vries, S., Kuenen, J.G. & Jetten, M.S.M., “Involvement of a novel hydroxylamineoxidoteductase in anaerobic ammonium oxidation,” Biochemistry, Vol. 39, 5405-5412
(2000).
Schalk, J., Oustad, H., Kuenen, J.G. & Jette n, M.S.M., “The anaerobic oxidation of hydrazine: a
novel reaction in microbial nitrogen metabolism,” FEMS Microbiology Letters, Vol. 158, 61-67 (1998).
Schmid M., Twachtmann U., Klein M., Strous M., Juretschko S., Jetten M., Metzger J.W.,
Schleifer K.H. and Wagner M., “Molecular evidence for genus level diversity of bacteriacapable of catalyzing anaerobic ammonium oxidation,” Systamatic Applied Microbiology,
Vol. 23, 93–106 (2000).
Schouten, S., Strous, M., Kuypers, M.M.M., Rijpst ra, W.I.C., Baas, M., Schubert, C.J., Jetten,M.S.M. & Sinninghe Damsté, J.S., “Stable Carbon Isotopic Fractionations Associated withInorganic Carbon Fixation by Anaerobic Ammonium-Oxidizing Bacteria,” Applied &
Environmental Microbiology, Vol. 79, 3785-378 (2004).
Shivaraman S. and Geetha S., “Anmmox – A novel microbial process for ammonium removal,”
Current Science, Vol. 84 (12). 1507-1508 (2003).
IN -110
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IN -111
Strous M., Fuerst J.A., Kramer E.H., Logemann S., Muyzer G., vandePas-Schoonen K.T., Webb
R., Kuenen J.G. and Jetten M.S., “Missing lithotroph identified as new planctomycete,” Nature, Vol. 400, 446–449 (1999).
Strous M., Kuenen J.G. and Jetten M.S.M., “Key physiology of anaerobic ammonium
oxidation,” Applied Environmental Micorbiology, Vol. 65, Pp. 3248–3250 (1999).
Strous, M., Fuerst, J.A. Kramer, E.H.M., Logema nn, S., Muyze, G., Van De Pas-Schoonen,K.T., Webb, R., Kuenen, J.G. & Jetten, M.S.M, “Missing litotroph identified as new
plantomycete,” Nature, Vol. 400,.446-449 (1999a).
Strous, M., Heijnen, J.J., Kuenen, J.G. & Jetten, M.S.M., “The sequencing batch reactor as a
powerful tool for the study of slowly growing anaerobic ammonium-oxidizingmicroorganisms,” Applied Microbiology & Biotechnology, Vol. 50, 589-596 (1999b).
Van De Graaf A.A., Mulder A., Bruijn P., Jetten M.S., Robertson L.A. & Kuenen J.G.,
“Anaerobic oxidation of ammonium is a biologically mediated process,” Appl.
Environ.Microbiol., vol. 61, 1246-1251 (1995).
Van de Graaf, A.A., De Bruijn, P., Robertson, L.A., Jetten, M.S.M. & Kuenen, J.G.,
“Autotrophic growth of anaerobic ammonium oxidation on the basis of 15N studies in afluidized bed reactor,” Microbiology, Vol. 143, Pp. 2415-2421 (1997).
Van Dongen L.G.J.M., Jetten M.S.M. & Van Loosdrecht M.C.M., “The CombinedSharon/Anammox process for treatment of ammonium rich wastewater,” Water Science and
Technology, vol. 44, Pp.153-160 (2001).