1. Getting Real Time Datain Drug Discovery usingRaturn and Culex Systemswith Biosensor-coupledAutomated SamplingLori Payne, Ph.D. VP Analytical & Discovery ServicesCPSA 5-Minutes of Fame October 3, 2012Contact: lpayne@basinc.com765.497.5894 Drug Development Services l Instrumentation

2. Standard CRO Services Preclinical Toxicology Discovery and GLP Pre- and Post- IND Enabling Bioanalysis Small and Large Molecule Pharmaceutical Analysis Stability Release In-vitro BE Drug Development Services l Instrumentation 2 3. BiosensorsCulex Dried Blood Spot (DBS)Drug Development Services l Instrumentation 3 4. Why BASi Equipment or Discovery Services? Automated sampling from a single animal single PK profile 24/7 coverage Microsampling or DBS/RRR Traditional samples or microdialysate/ultrafiltrate PK/PD at the same time In-line, on-line, real time making decisions onthe fly Innovation www.basinc.com Linkedin CULEX USER FORUMDrug Development Services l Instrumentation 4 5. Sampling PumpBlood/Serum/PlasmaBileEmpis In-vivo Dialysates, UltrafiltratesInfusion PumpRefrigerated BloodSample Storage UnitRaturnTelemetryReceiverMetabolic WasteCollection System Telemetry Antenna Heart Rate, BP/LVP Temperature, Locomotion EMG, ERG, ECG or EEGDrug Development Services l Instrumentation 5 6. Biosensors Key Features Linear response over a wide, physiologically-relevant concentrationrange Exclusion of endogenous electroactive interferents present in thebrain Fast response (1-4s) capable of monitoring the time course of aphysiological release Small, rigid design for precise stereotaxic placement in the brain Uses In vivo monitoring of brain chemical microenvironments Monitoring of seizure and addiction related neurochemical events Neurochemical monitoring of behavior and physiologic correlates Drug screening, including neuropharmacological effectsDrug Development Services l Instrumentation 6 7. Drug Development Services l Instrumentation 7