suggest that SOX3-expressing cells within the CNS control pitui-

tary endocrine cell size and consequently hormone secretion.

doi:10.1016/j.mod.2009.06.304

07-P022

Applications of lentiviral transgenesis in the chick

Feifei Song, Adrian Sherman, Isabelle Worthington, Mike

McGrew, Helen Sang

Roslin Institute, University of Edinburgh, Edinburgh, United Kingdom

We have previously shown that lentiviral vectors can be used

to generate transgenic chickens efficiently and that expression of

introduced transgenes is not silenced on transmission through

the germline. Transgene expression can be targeted in the pre-

dicted tissue-restricted manner, for example: muscle-specific

expression is restricted to skeletal muscle using the rat myosin

light chain 3 gene enhancer/promoter and oviduct-specific

expression is demonstrated using regulatory elements of the

chicken ovalbumin gene. We also produced transgenic chicken

lines expressing GFP ubiquitously using the CMV IE enhancer

fused to the chicken b-actin promoter/intron. High levels of GFP

protein are visible at all stages of developing transgenic embryos.

GFP embryos have been wildly used in cell-fate experiments

where the grafted cells can be visualised and followed during

embryo development. Here we are extending the repertoire of

reporter genes with the development of transgenic lines that

ubiquitously express membrane-localized GFP or monomeric-

Cherry (red), which will make invivo tracing cell shape changes

and cell interactions possible. Founder males chimeric for trans-

genes coding for membrane-localized fluorescent proteins have

been raised to sexual maturity and crossed to screen for trans-

genic offspring. In addition, we are generating transgenic lines

carry photo-activatable GFP or photo-switchable CFP that should

allow marking of cells insitu for lineage tracing studies without

the need for grafting.

doi:10.1016/j.mod.2009.06.305

07-P023

GUDMAP – An online genitourinary resource

Simon Harding1, Jamie Davies2, Jane Armstrong2, Jane Brennan2,

Sue Lloyd-MacGlip2, Derek Houghton1, Mehran Sharghi1, Xingjun

Pi1, Ying Cheng1, Bruce Aronow3, Sean Grimmond4, Peter

Koopman4, James Lessard3, Melissa Little4, Andy McMahon5,

Cathy Mendelsohn6, Steve Potter3, Michelle Southard-Smith7,

Duncan Davidson1

1MRC Human Genetics Unit, Edinburgh, United Kingdom2University of Edinburgh, Edinburgh, United Kingdom3Cinncinati Children’s Hospital Medical Center, Cincinnati, OH, United

States4University of Queensland, Brisbane, Australia5Harvard University, Cambridge, MA, United States6Columbia University Medical Center, New York, NY, United States7Vanderbilt University Medical Center, Nashville, TN, United States

The GenitoUrinary Development Molecular Anatomy Project

(GUDMAP) is a consortium of laboratories working to provide

the scientific and medical community with gene expression data

and tools to facilitate research (see www.gudmap.org). The data

provided by GUDMAP includes large insitu hybridization screens

(wholemount and section) and expression microarray analysis

of components of the developing mouse urogenital system

(including laser-captured material and FACS-isolated cells from

transgenic reporter mice). In addition, a high-resolution anatomy

ontology has been developed by members of the GUDMAP consor-

tium to describe the subcompartments of the developing murine

genitourinary tract.

The GUDMAP Database Development and Editorial teams,

both based in Edinburgh, function to ensure submission, cura-

tion, storage and presentation of the data submitted by the GUD-

MAP consortium. Thus far, we have developed a range of tools

that help both the submitter and the end user. These include:

an online annotation tool that simplifies insitu data submission

through an ontology-based graphical user interface; a database

interface that allows users to browse and query expression data,

and to filter data by organ system; a heat-map display of micro-

array data and analyses. Furthermore, the Edinburgh team has

developed a GUDMAP Disease Database that queries associations

between genes, genitourinary diseases, and renal/urinary and

reproductive phenotypes.

By virtue of its impressive, high-resolution dataset and its ease

of use we hope that the GUDMAP Website will continue to serve

as a powerful resource for biologists, clinicians and bioinformati-

cians with an interest in the urogenital system.

doi:10.1016/j.mod.2009.06.306

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