1 10 ml of tsb source vial of tsb 20 or 30 ml shielded evac vial (simulated tc-99m) x 4 simulated...
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1
10 mL of TSB
SourceVial of TSB
20 or 30 mL shielded evac vial
(simulatedTc-99m) X 4
Simulated Kit vial #1
1 mL
3 mL
(repeat 3 moretimes for a totalof 4 vials)
3 mLShielded syringe
10 mLunshielded syringe
Draw 0.4 mL of media from Kit
Media Fill Procedure
(simulated normal saline)
2
3 mL shielded syringes
10 or 20 mL incubation vials
Draw 0.4 mL of media from Kit
Media Fill Procedure (Cont’d)
Kit # 1 Kit #2 Kit #3 Kit #4
Repeat 3 times for a total of 4 vials and 24 syringes
Repeat 3 times for a total of 4 vials and 24 syringes
3
Media Fill Procedure (Cont’d)
• Incubate 4 new vials at 25-35 Degrees Celsius in a controlled environment for 14 days.
2 3 41
10 mL of TSB
Vial of TSB
20 or 30 mL shielded evac vial
Tc-99m X 4
Simulated Kit vial #1
1 mL
3 mL
(repeat 4 times)
5 mLShielded syringe
10 mLunshielded syringe
3 or 5 mL shielded syringes
Draw 0.4 mL of media from Kit
Incubate vials at 25-35 Degrees Celsius
Media Fill Procedure (Overview)
10 or 20 mL incubation vials
1
Repeat process 4 times for a total of 24 incubation vials