1 che-554 biological chemistry lab - university of kentucky€¦ · che-554 biological chemistry...
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© A.-F. Miller 2014
CHE-554 Biological Chemistry Lab
Tues and Thurs, 12:30 pm CP 222 & 1:30 or 3:30 in CP 236
Bring lab coat, safety glasses, USB memory stick, notebook.
Wear sensible clothing: closed-toe shoes, no flammable
synthetics or loose items.
Be prepared in advance for each day.
COME
Watch for these techniques in the literature, and the news.
Why did you choose this course ?
Syllabus, and more, will be available at:
www/chem.uky.edu/courses/che554
Before the site is ready, handouts will be distributed through
email.
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SAFETY in the Biological Chemistry Lab
Prof. Wei
January 2015
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SAFETY in the Biological Chemistry Lab
Don’t work alone unless the task is genuinely routine for you.
– Tired, bored, stressed, distracted
– Don’t have the correct tools
– Other ?
When do accidents happen ?
– Attitude
– Alert
– Prepared
– Skill and experience
– Correct Equipment
– Equipment is in good condition
– Good environment (lighting, distractions).
Your safety equipment:
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General Laboratory Safety
The biological chemistry laboratory can be a
dangerous place if:
You are not being careful and not paying attention to
what you are doing.
You do not understand what you are doing.
You do not use equipment or techniques properly.
https://www.youtube.com/watch?v=s6lOQ5_Vlok
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Authorized Activities and Proper Conduct
It is very important that:
You follow the operating procedures suggested in the handouts,
the textbook, and in equipment manuals.
You only perform the assigned experiments.
You pay attention to the direction and warnings of your supervisor
or instructor.
You respect the safety of others in every laboratory operation that
you perform.
Establish a buddy system, and use it.
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Most common reasons experiments fail
Errors following the protocol
– Write and diagram out an action plan for the entire experiment,
compiling a list of required reagents and equipment, IN
ADVANCE.
– Check steps off as they are completed, and WRITE DOWN
observations regarding the condition of the samples at each
step (write down the time as well).
– Keep an eye on total volumes, these will reveal when a reagent
has not been added, or been added in the wrong quantity.
– Check all solutions, before using them (eg. confirm pH).
This will be done for you.
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Dress Code Safety glasses must be worn at all times. It is recommended that you not
wear contact lenses in the laboratory.
Minimize skin exposure. Legs and feet should be completely covered.
Long pants, skirts, dresses, etc. must cover legs and ankles. Shorts, capri
pants, tank tops, midriff shirts, muscle shirts, open-toed shoes, and
sandals are not permitted.
Wear a lab coat at all times.
Wear exam gloves at all times.
Tie back or confine long hair and loose items of clothing and jewelry.
Choose non-flammable clothing.
Turn off any distractions: cell phones etc. NEVER wear head phones in a
lab.
Do not even enter the room if you are under the influence of any disruptive
substance: alcohol, sedatives, drugs, etc.
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Flammable Materials
Keep flames away from the area where you use
ether, acetone, methanol, ethanol or other organic
solvents.
Handle flammable solvents in a fume hood.
Do not inhale chemical vapours. They can be
deadly.
Wear non-synthetic clothing and shoes that provide
a barrier to liquid penetration (athletic shoes w.
mesh top and sides fail this test).
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Biohazards
Your resident bacterial flora can contaminate your
experiment.
Dyes such as ethidium bromide are carcinogens
with delayed but serious toxicity.
Gloved hands are research hands. Research hands
do research, ONLY: no hair adjustments, wiping
face, use of public facilities ... (avoid using your
keyboard or cell phone).
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Spills
All spills must be cleaned immediately (biological or
chemical).
Biologicals or chemicals spilled on the skin should
be washed off immediately by flushing with water for
15 minutes. Notify T.A. immediately.
In case of a major accident, seek medical treatment
immediately. (Also notify T.A. and instructor.)
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Waste Disposal Dispose of all waste properly. Waste containers will be provided. If
unsure, ask your supervisor or instructor.
Fully read the labels on chemical and waste containers before
disposal.
Do not forget to close the container lid when done. For volatile
substances, do not tighten the lid.
Biohazard materials must be disposed off in appropriate bags.
Infectious Material must be disposed of in red bags.
Sharp materials such as gel cutters, razor blades, and syringe
needles much be disposed off in “sharps” container.
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Concentrated Acids and Bases
When handling acids or bases:
Be careful to avoid splashing them on skin or clothes.
Dilute by adding the reagent to water (never add
water to the reagent).
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Toxic Chemicals Toxic chemicals such as acrylamide, caustic solutions, cyanides,
oxalates, mercury salts, are hazardous to humans.
Never handle such chemicals such that your skin is in direct contact with them.
Never mouth-pipette anything.
Use impermeable gloves and nose mask while weighing or using these chemicals.
Wash any chemical spills off your body promptly.
Wash your hands with soap after EVERY session in lab, regardless of whether any mishap occurred.
Familiarize yourself will all reagents beforehand.
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Safety of reagents
MSDS
Merck Manual
Web sites
http://cartwright.chem.ox.ac.uk/hsci/chemicals/aspiri
n.html
provides a simplified version. Authentic ones for
aspirin and glucose are available via the course web
site.
Also go through www.sigmaaldrich.com/united-
states
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Safety of reagents
MSDS
– often prepared with the intension of providing legal
protection to the manufacturer, rather than practical
information for the user.
– Look at the LD50, IC50
– Know these values as they apply to a human (you !)
For dangerous materials
– Know exposure routes
– Know protective devices and protocols
– Know antidotes and first-response actions.
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Safety Equipment
Know the location of all safety equipment
– Showers
– Eye washes
– Fire extinguishers
– First aid kit
– Evacuation plan
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Handling Glass
Glass cuts are among the most common lab
accidents. Even the most experienced chemists are
susceptible. Please be careful.
Do not insert glass tubing into rubber stoppers or
corks.
Discard cracked and chipped glassware in glass
disposal box.
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Reporting Accidents
Report an accident or an injury, no matter how
minor, to the instructor or laboratory supervisor
immediately. An accident report form must be
completed.
A first aid kit for minor cuts or burns is available in
the laboratory. Ask the instructor or laboratory
supervisor for help.
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Safety Information
In Case of an Emergency
– Administer First Aid
– Stay with the injured person
Call 9-911
Important Phone Numbers:
– UK Police Dept. (7-8573)
– Environmental Health and Safety (7-3241)
– Occupational Health and Safety (7-3827)
– Hazardous Materials Management (3-6280)
Accident Report Form
– www.uky.edu/FiscalAffairs/Environmental/ohs/
Adhere to Building Emergency Evacuation Plan.
– Take note of your locker-mate’s successful evacuation.
– Stay together as a class to facilitate accounting for all members.
20 Section1 Lockers & Groups
Name Locker Group Bale, Michael Javier L28C A
Bosshammer, Samantha Leigh L28C B
Combs, Travis Allen L28C C
Do, Christy Luc L31C A
Hayden, Christopher Stephen L31C B
Hempel, Karl Willis L31C C
Holdeman, Kyle Robert L34C A
Hong, Calvin Ying L34C B
Howell, Nicholas Randolph L34C C
Huynh, Bobby L L35C A
Kelly, Thomas Francis L35C B
Lammers, Erin Elizabeth L35C C
Mattingly, Jacob Michael L38C A
Nguyen, Kim D L38C B
Phillips, Jared Allen L38C C
Sandell, Katelyn L41C A
Tran, Angela L41C B
Yu, Samuel Shung-Bon L41C C
L44C A
L44C B
L44C C
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Section2 Lockers & Groups Name Locker Group
Lee, Sunyoung L28C A
L28C B
L28C C
Lim, Leang L31C A
L31C B
L31C C
Nagy, Tamas L L34C A
L34C B
L34C C
Voss, Gareth James L35C A
L35C B
L35C C
Fortenbery, Zachary Ryan L38C A
L38C B
L38C C
L41C A
L41C B
L41C C
L44C A
L44C B
L44C C