1

DIEHARD Mycorrhizal Inoculants

Prepared For

Rainbow Treecare

Scientific Advancements By: Horticultural Alliance, Inc.

ContactJim Quinn800-628-6373jamesq@horticulturalalliance.com

AL

LIA

NC

E

2

Horticultural Alliance, Inc.• Technology Based

– Innovators in application technology• Service Lead

– Our goal is to serve YOU and your business

• Customer Driven– Our products and service have evolved by

listening to our customers

3

What are Mycorrhizas? Mycorrhizas are highly evolved, mutualistic associations between soil fungi and plant roots. Mycorrhizal associations involve 3-way interactions between host plants, mutualistic fungi and soil factors.

4

Benefits of Mycorrhiza

• Survival

• Rooting

• Water Absorption

• Nutrient Availability

• Soil Aggregation

• Boosts Micro-Flora Activity

5

Types of Mycorrhizas

• At least seven different types of mycorrhizal associations have been recognized, involving different groups of fungi and host plants and distinct morphology

• Only endo- and ectomycorrhizas will be presented here. These two account for over 99% of plants on earth.

6

Mycorrhizal Fungi• Endomycorrhiza – 7* strains to include

Glomus mosseae, Gigaspora margarita, Glomus brasilianum, Glomus deserticola, Glomus clarum, Glomus etunicatum, and Glomus intraradices.

• Ectomycorrhiza – 9 strains to include Pisolithus tinctorius, four species of Rhizopogon, two species each of Laccaria and Scleroderma.

* Glomus sp (sclerocystis specie) and Glomus fasiculatum to be added 3nd quarter 2001.

7

Endomycorrhiza (AM) Vesicular-arbuscular mycorrhizas are associations within roots. Spores are formed in soil or roots.

Brundrett, 1984

8

Photo Collection of Endomycorrhiza

Spores inside the roots.

Dr. David Sylvia, Univ. of Florida, Gainesville, FL

9

Photo Collection of Endomycorrhiza

Arbuscule in a root fluorescing from antibodies produced abundantly by fungal hyphae that aids considerably in soil aggregation.

.

Dr. David Sylvia, Univ. of Florida, Gainesville, FL

10

Production of Endomycorrhiza

Endomycorrhiza is propagated in a greenhouse and the roots are harvested along with spores and hyphae.

Dr. David Sylvia, Univ. of Florida, Gainesville, FL

11

Collection of Ectomycorrhiza in Nature

Ectomycorrhiza is harvested in the form of “puff balls” in native forests and processed to become an inoculant.

12

Applications

• In The Nursery

• In The Field

• Established Plants

13

In The Nursery

Inject an inoculant containing mycorrhiza and water gel in nursery containers.

14

In The Field

As a soil amendment when planting nursery stock.

15

Established Trees & Shrubs

As deep root injection.

16

Advantages Of DIEHARD Mycorrhizal Inoculants

Ingredients

Multiple Strain Diversity

Size of Production Runs

Quality Control

YOU are our business partner

17

Ingredients In DIEHARD Mycorrhizal Inoculants

• Mycorrhizae – we source from 5 different suppliers in the United States and Europe

• Biostimulants – proprietary blend of the highest quality ingredients available anywhere

18

Mycorrhizal Fungi

• We DO NOT use ONLY SPORES but instead use spores, hyphae, and root fragments. Research at INVAM at the University of West Virginia shows that spores are the least infective propagules followed by hyphae and root fragments respectfully.

19

Biostimulants – proprietary blend of the highest quality ingredients available anywhere

• Humates – only the best from the Dakotas

• Yucca Extracts – highest quality anywhere, from Mexico

• Seaweed Extracts – only the best from the Norwegian coast

20

Size of Production Runs

• Small – generally 1,000 to 2,000 pound runs

• Assures you of “fresh” inoculum that hasn’t been sitting in a warehouse for months, or even years.

• We turn raw materials inventory 8-14 times per year.

21

Quality Control

• All inoculum from suppliers are tested for infectivity potential before they are used to make your products.

• Certificates of analysis are required from all suppliers on each order from us to insure that you consistently get the best ingredients available.

22

Products Offered The Professional Arborist

• DIEHARD Transplant • DIEHARD Injectable • DIEHARD Ecto Injectable • DIEHARD Root Reviver • DIEHARD BioRush Biostimulant• DIEHARD BioRush Fe Biostimulant with iron

23

Inoculant “Cocktails” Contain

Endo & Ectomycorrhizal Fungi

Complete Biostimulant Package

Trichoderma

54+ Strains Beneficial Bacteria

Yucca Plant Extract

Seaweed Plant Extract

24

- Profit Margins - RTC Cost

DIEHARD Product MSRP Cost*

DIEHARD Transplant $199.50

DIEHARD Root Reviver $64.95 30-37%

DIEHARD Injectable Cs.

DIEHARD Ecto Inj. Cs. 45%

DIEHARD BioRush Cs. 33-44%

DIEHARD BioRush Fe Cs. 33-40%

*Delivered in 48 unit pallets

25

Services We Offer

• Immediate Response 800-628-6373• Toll-Free Fax Number 888-386-4478• Extensive Web Page (Over 16 megs)• Interactive Specification and Usage

Template On-Line• Custom Inoculant based upon your

needs/conditions• Complete laboratory work (at cost)

26

SummaryHIGH QUALITY PRODUCTS AND SERVICE

• Result in a hassle-free business partnership

• Enable more reliable results in the field.• Enhances your customer goodwill • Expands potential customer base through

word-of-mouth satisfaction/results

27

Thank You

For Making This Presentation Possible

28

Technology Based * Service Lead * Customer Driven

AL

LIA

NC

E