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    Receiving and Storing

    U. S. Grain at Destination

    Carl Reed

    International Grains Program

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    Grain Export from the U. S.

    Trigo

    Maz

    Soya

    Sorgo

    Inland Export

    Route New

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    Exported U. S. CornAverage m.c. 14.3 %

    Average TW 72.8 kg/hl

    Average BCFM 2.7 %

    Average Damage 2.7 %

    M.C. other grains

    Wheat 11.5 13.0%

    Sorghum 13.5%

    Soybeans 12.2%

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    Receiving and Storing U. S. Corn

    Contract specifications are critically

    important.

    If the grain is to be stored in a warmclimate, special precautions must be taken

    Dealing with moisture variations

    Dealing with variations in broken kernels and

    fine material

    Quality maintenance in storage (>6 weeks)

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    Dealing with Moisture Variation

    If grain must be stored.

    Segregate by moisture at reception Use wetter grain first

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    Example of Moisture Variation

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    Ship Date Truck Temp m.c.Pacific Bulker 5/25/00 1 26 14.0

    Pacific Bulker 5/25/00 2 25 15.1Pacific Bulker 5/25/00 3 24 15.0

    Pacific Bulker 5/25/00 4 25 14.9

    Pacific Bulker 5/25/00 5 24 14.8

    Pacific Bulker 5/25/00 6 25 14.7

    Pacific Bulker 5/25/00 7 26 14.6

    Pacific Bulker 5/25/00 8 25 14.7

    Pacific Bulker 5/25/00 9 25 14.7

    Pacific Bulker 5/25/00 10 24 13.4Pacific Bulker 5/25/00 11 24 14.1

    Pacific Bulker 5/25/00 12 25 13.9

    Pacific Bulker 5/25/00 13 24 13.7

    Pacific Bulker 5/25/00 14 22 14.7

    Pacific Bulker 5/25/00 15 23 15.1

    Pacific Bulker 5/25/00 16 23 14.7

    Pacific Bulker 5/25/00 17 22 14.5

    Pacific Bulker 5/25/00 18 22 15.0Pacific Bulker 5/25/00 19 23 15.7

    Med Faith 6/21/00 1 22 13.9

    Med Faith 6/21/00 2 23 14.7

    Med Faith 6/21/00 3 21 13.8

    Med Faith 6/21/00 4 22 14.3

    Med Faith 6/21/00 5 22 14.1

    Med Faith 6/21/00 6 21 14.4

    Med Faith 6/21/00 7 22 14.7Med Faith 6/21/00 8 22 14.9

    Example of Moisture Variation

    *

    *

    *Compaia

    CentroAmericana

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    Dealing with Fine Material in Corn

    Sample and analyze to know FM level

    Clean before storage, or Core bins before storage

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    Dealing with Insects in Any Grain

    Recognize that commercially-handled grain is

    infested Insect density has to be high before the infestation

    can be detected

    More insects are tolerated in feed grains than in

    wheat May fumigate en-route, or

    Fumigate at destination

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    Life Cycle of Stored Product Beetles

    Eggs

    Larva

    Pupa

    Adult

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    Life Cycle of Stored Product Beetles

    Eggs

    Larva

    Pupa

    Adult

    These forms are invisible ingrain samples

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    Laboratory Study on

    Probability of Detection

    010

    20

    30

    4050

    60

    70

    80

    0 1 2 3 4 5 6

    Insect Density (#/kg)

    Proba

    bilityofDetection(%)

    Hagstrum et al. 1985. Environ.

    Entomol 14:655-661

    The USDA study showed that

    when 1 insect/kg was present,

    a single, 1-Kg sample had a 1-

    in-3 chance of containing an

    insect.

    The study showed that the

    probability of detecting insects

    is about 3 times smaller than

    what probability alone would

    predict.

    Si ifi f S li

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    Significance of Sampling

    Probability Numbers There are 1,000 one-kg samples in a MT of corn

    If the insect density is 1/kg, then there are 1,000insects per MT

    If the lot subsample represents 120 MT, and thegrain contains 1 insect/kg, there are 120,000insects in the grain represented

    The probability of detecting insects if a one-kgsample is taken is one in three

    If the true insect density is only one in 10 kg,thenthe probability of detection is one in 30.

    If only one of every 10 insects in an adult, the

    probability of detection is one in 300

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    Dealing with Mold-Produced

    Deterioration

    Segregation by moisture at reception

    Control of fine material Use of mold inhibitors

    Maintain cool temperatures

    If grain arrives cool, maintain cool temperature Use aeration if needed

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    Common field fungi including species ofthe generaFusarium, Cephalosporium,

    Cladosporium, etc. are common in the

    soil and inside plants of growing corn.

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    The field molds require a high moisturecontent in their environment. By the time

    corn has dried enough to harvest, the field

    molds have become dormant.

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    Grain arrives at storage infected with dormant field

    molds. In handling equipment and bins it is inoculated

    with a new group of molds, those that can survive in

    dry grain in storage.

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    Molds and Mycotoxins

    Myco = mold Aflatoxin produced

    byAspergillus flavusand others.

    Zearalenone produced by Fusariumroseum and others

    DON (vomitoxin) one of several tricho-thecenes produced byFusarium tricinctumand others.

    Almost always produced

    on the plant*, though the

    fungus can grow in storage.

    Field fungus toxin

    produced in the field.*

    Field fungus toxin

    produced in the field.*

    *Or field-type situation

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    Molds and Mycotoxins - 2

    T-2 another tricho-thecene produced byFusarium molds

    Fumonisin produced byFusarium moniliforme andothers

    Ochratoxin produced byand Penicillium species,

    Aspergillus ochraceus, andothers

    Field fungus toxinproduced in the field.*

    Field fungus toxinproduced in the field.*

    Both field and storagefungi, toxin productionappears associated withstorage of cool, dampgrain

    *Or field-type situation

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    Field-Type Situation?

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    Field-Type Damages

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    What does grain-mold biology mean

    for importers of US grain? At harvest, the US domestic market quickly

    identifies the origin of any grain containingmycotoxin

    Exporters attempt to avoid grain from thatproduction area

    Exported corn is assayed for aflatoxin, and anygrain can be tested for toxins at the purchasersrequest

    Exported grain is dry, so toxin can not beproduced in the grain unless it gets wet

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    Root of Infected Embryo

    Mold Spores and Hyphae

    Eff t f G i M i t

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    Effect of Grain Moisture on

    Growth of Storage Molds

    0

    20

    40

    60

    80

    100

    120

    0 2 4 6 8 10 12 14

    Weeks at 25 C

    A.glaucusInfect

    ion(%)

    18 % m.c.16.5 % m.c.14.6 % m.c.Linear (14.6 % m.c.)Linear (16.5 % m.c.)Linear (18 % m.c.)

    Effect of Temperature on

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    0

    5

    10

    15

    20

    25

    15 16 17 18 19 20 21 22 23 24 25

    Temperature ( C)

    Monthsto0.5

    DML

    14 % m.c.

    15 % m.c.

    16 % m.c.

    Effect of Temperature on

    Growth of Storage Molds

    59 F 77 F

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    y = 1.4x + 3.3

    R2 = 0.95

    0

    10

    20

    30

    40

    0 4 8 12 16 20

    Weeks of Storage

    Dam

    agedKernels(%)

    Bin Avg

    Linear (Bin Avg)

    Increase in damaged kernels in corn at

    14.6 % m.c. and 24 28 C75-82 F

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    0

    20

    40

    60

    80

    0 2 4 6 8 10 12

    Weeks at 25 C

    Inte

    rnalInfection(%)

    A. glaucus

    Fusarium

    Increase in Infections byA. glaucus and

    Decrease inFusarium spp. at 14.6 % m.c.

    D i T l M ld C f 18 0 %

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    Decrease in Total Mold Counts of 18.0 %-m.c.

    Corn on General-Purpose Agar over Time

    0

    20

    40

    60

    80

    100

    0 2 4 6 8 10 12

    Weeks at 25 C

    InternalInfection(%)

    025

    50

    75

    100125

    150

    175

    200

    cfu/g(00

    0)

    All Aspergillus spp.

    Rose Agar

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    Oxygen Consumption Over Time (averages by mold inhibitor and dose)

    0.0

    0.1

    0.2

    0.3

    0.4

    0.5

    0.6

    0.7

    0.8

    0.9

    0 5 10 15 20 25 30 35 40 45 50

    Days of Incubation

    mgO2/hr/kgDM

    Untreated

    Prod B 0.1 kg/tProd A 0.1 kg/t

    Respiration of Pellets

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    Corn at 15.0 % m.c. Treated with

    Product A

    0.0

    1.0

    2.0

    3.0

    4.0

    5.0

    1 11 21 32 42 54

    Days of Incubation

    mgO2/h/kgDry

    Untreated

    0.5 kg/t

    1.0 kg/t

    2.0 kg/t

    3.0 kg/t

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    Aeration

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    Effect of Grain Temperature

    on Rate of Deterioration

    60

    80

    100

    120

    140

    160

    180

    0 2 4 6 8 10Weeks of Storage

    Perc

    entKernelsInfecte

    dbyMolds*

    * Percent Infection

    as % of Original 14.3 % m. c. and 86 F

    14.3 % m. c. and 77 F

    R d ti f A ti f

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    Recommendations for Aeration of

    Imported Corn* in Warm Climates

    Do not aerate

    If the grain is cooler than 20 C and the graintemperature is stable

    When the air is as warm or warmer than the grain

    Aerate only when air is at least 3 C cooler thanthe grain

    Monitor aeration with grain temperature

    monitoring system, stop as soon as cooling iscomplete

    Only if the grain is heating should aeration beused regardless of the air temperature and RH

    *stored less than 3 months

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    Receiving and Storing

    U. S. Grain at Destination

    Carl Reed

    International Grains Program