1 lecture 5: linkage. 2 linked genes, recombination, and chromosomal mapping mendel's law of...
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Linked genes, recombination, and chromosomal mapping
Mendel's Law of Independent Assortment is a consequence of the fact that chromosomes segregate independently in meiosis
Take two individualsOne heterozygous and one homozygous
AaBb x aabb
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Chromosome alignment in MeiosisI
These results are readily explained by the two alternative ways the chromosomes can line up on the metaphase plate during meiosis I:
A a
B b
OR
A a
b B
AB ab Ab aB
Because the A and B genes assort independently, AaBb dihybrids constructed from different parental genotypes will behave the same.
AABBxaabb AAbbxaaBB
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Why 50:50Why not 25:25:25:25
A dihybrid cross involving the genes A and C produced the following results:
· A= Tall a= short
· C= Cream c = white
Cross I: Cross II:
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In these crosses, independent assortment is not occurring.
For example in the first cross, the alleles Tall and Cream behave as if they are linked to one another. Similarly in the second cross the alleles Tall and white appear as if they are linked to one another.
These results are readily explained if the genes A and C lie next to one another on a chromosome:
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Purple vestigial
Morgans experiment in Drosophila to determine if the genes pr and vg were linked.
PR+ = normal red eyes pr = purple eyes
VG+ = normal wings vg = vestigial wings
P
F1
If they are on different chromosomes they should assort independently
If they are next to one another on the same chromosomes they should not assort independently
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The chromosomes that have gone through this crossover are known as crossover products or recombinants. The original chromosomes and those that have not undergone a crossover are known as parental.
Evidence for the model that chromosomes physically exchange during meiosis is found in meiotic structures known as chiasmata.
During meiosisI when homologs pair, non-sister chromatids appear to cross with each other. The resulting cross-shaped structure is known as a chiasmata.
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Crossing-over through the microscope
Duplicated homologous chromosomes
Synapsis
Crossing over betweenNon-sister chromatids
AnaphaseISegregation of homologouschromosomes Haploid gametes
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Answer:
To explain this we need to define the terms parental and recombinant:
Parents:
Gametes:
F1:
Meiosis produces the following gametes:
How does one determine whether two genes reside on different chromosomes or reside on the same chromosome as linked genes?
Recombinant gametes are those with different allelic combinations than those gametes of the previous generation.
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Coupling/repulsion
Genes located on the same pair of homologous chromosomes are called LINKED GENES
Therefore when the A and C alleles are introduced from one parent they are physically located on the same chromosome and they do not assort independently. We say that they are linked.
In the above cross we say that the A and C genes are linked.
Therefore when we write the genotype of a dihybrid for two linked genes, there are two possible conformations:
AaCc
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Recombination frequency
If a crossover occurred between linked genes each time homologs paired, the recombinant frequency would be 50%
This is because crossing-over involves only two of the four chromatids on the metaphase pair (each of the paired homologs consists of two sister chromatids).
For example, the frequency of recombinant gametes between linked genes A and B is 50% if crossing-over occurred each time the homologs paired.
A B
A Ba b
a b
A B
A b
a B
a b
parental
parental
recombinant
recombinant
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However there are many instances in which the homologs pair and crossing over does not occur between genes A and B.
It occurs somewhere else
Consequently the overall frequency of recombinants is significantly reduced from 50%
A B
A Ba b
a b
A B
A B
a b
a b
parentalparental
parental
parental
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Distance
The larger the distance between two genes residing on the same chromosome, the higher the probability there is that a crossover event will occur between them.
That is for any chromosome, there is a fixed probability per a given distance on the chromosome that a crossover event will event.
Sturtevant realized that this property could be used to map genes with respect to one another. For each pair of genes on a chromosome a recombination frequency can be determined.
By determining the recombination frequency between many pairs of genes on a chromosome, the relative distance between genes and the order of the genes on the chromosome can be determined.
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For example Sturtevant identified three recessive mutations that reside on the X chromosome of Drosophila
By calculating recombination frequencies between each pair of
genes we can begin to establish where these three genes reside
on the X chromosome with respect to one another
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To determine the distance between the w gene and the sn gene
P w sn/w sn x W+ SN+/Y
F1 w sn/W+ SN+ x w sn/Y
F2
Fill out the phenotypes- recombinants can be determined by phenotype analysis
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Recombination frequency equals the number of recombinants over total number of progeny
# recombinant progeny =
# total progeny
1 map unit (m.u.) = 1% recombination frequency
Therefore _________________separate the w and sn genes.
This is a relative distance- depends upon recombination between two genes. Not an absolute distance like bp
In the above cross, we could have determined recombination frequency by counting only males (or only females)