12,12 first cycle (1).xls

7/27/2019 12,12 First cycle (1).xls

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Nitrates

Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Day 8

Control 53.942 47.882 38.742 32.842 24.742 17.674 10.942 10.942

5g 57.325 42.048 35.049 28.994 20.843 12.045 7.449 7.449

10g 54.464 46.943 41.994 35.053 26.853 23.945 15.965 15.965

15g 55.949 31.043 21.535 14.995 8.667 2.943 1.585 1.58520g 52.631 45.942 34.604 19.003 14.783 7.943 3.743 3.743

Nitrates


Control 52.968 45.432 38.586 30.474 25.854 15.943 11.954 11.954

5g 55.432 42.853 33.038 26.865 19.742 14.428 7.485 7.485

10g 53.472 45.842 40.942 36.048 24.583 22.942 16.758 16.758

15g 54.753 30.058 23.542 14.657 7.668 2.643 1.674 1.674

20g 53.853 44.753 34.953 28.742 13.532 7.485 3.584 3.584

LIPID

befor growth phase After growth phase end of starvation phase

Total Lipid Neutral lipi Total Neutral Total Neutral

Control 1.8 0.6 2.78 0.9 4.87 1.47

5g 1.8 0.6 16.6 4.5 28.4 9.743

10g 1.8 0.6 22.4 10.9 34.17 13.34

15g 1.8 0.6 19.4 8.8 30.406 11.5

20g 1.8 0.6 18.3 1.9 30.221 8.1

0

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20

30

40

50

60

70

1 2 3 4 5 6 7 8 9 10 11 12 13

Nitrates(mg/l)

Days

Control 5g

10g 15g

20g

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60

1 2 3 4 5 6 7 8 9 10 11 12 13

Nitrates(mg/l)

Days

Control 5g

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20g

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Phosphates

Day 9 Day 10 Day 11 Day 12 Day 13 Day14 Day15 Day 1

1.485 0.784 0.325 0.201 0.184 0.085 0.042 Control 40.842

3.942 1.032 0.882 0.425 0.218 0.108 0.046 5g 42.884

7.535 3.443 1.942 0.853 0.424 0.158 0.093 10g 44.774

0.321 0.094 0.054 0.0325 0.013 0.008 0.005 15g 42.6321.785 1.085 0.083 0.042 0.021 0.014 0.008 20g 40.748

Phosphates

Day 9 Day 10 Day 11 Day 12 Day 13 Day14 Day15 Day 1

2.842 0.685 0.378 0.198 0.185 0.082 0.036 Control 41.836

3.274 1.085 0.752 0.385 0.217 0.114 0.05 5g 42.773

6.853 2.853 1.853 0.832 0.413 0.143 0.09 10g 43.596

0.328 0.094 0.058 0.0328 0.016 0.001 0.006 15g 42.374

1.542 1.048 0.084 0.048 0.017 0.015 0.009 20g 40.586

Total Lipid

Control 5g 10g 15g 20g

Befor grow 1.8 1.8 1.8 1.8 1.8

After grow 2.78 16.6 22.4 19.4 18.3

End of star 4.87 28.4 34.17 30.406 30.221

Neutral Lipid


Befor grow 0.6 0.6 0.6 0.6 0.6After grow 0.9 4.5 10.9 8.8 1.9

End of star 1.47 9.743 13.34 11.5 8.1

14

0

5

10

1520

25

30

35

40

45

50

1 2 3 4 5 6 7 8 9 10 11 12 13 14

Phosph

ates(mg/l)

Days

Control 5g

10g 15g

20g

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140

5

10

15

20

25

30

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45

50

1 2 3 4 5 6 7 8 9 10 11 12 13 14

Phosphates(m

g/l)

Days

Control 5g

10g 15g

20g

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Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Day 8 Day 9 Day 10

35.042 28.784 22.945 14.894 12.849 5.748 5.748 2.272 0.893

36.288 28.294 20.932 14.392 7.842 3.704 3.704 0.758 0.313

35.839 27.603 17.845 12.49 8.424 3.473 3.473 1.073 0.732

38.893 33.849 26.893 18.484 13.743 7.434 7.434 3.894 0.85834.858 27.784 19.747 11.847 8.853 3.683 3.863 0.742 0.421


36.075 27.685 22.723 14.742 12.328 5.853 5.853 2.385 0.859

34.873 28.546 20.748 15.654 7.439 3.549 3.549 0.746 0.308

35.574 25.974 16.694 12.742 7.974 3.853 3.853 1.064 0.785

38.659 32.648 26.669 17.447 13.338 7.586 7.586 3.686 0.836

33.967 27.594 20.852 15.586 12.684 3.475 3.475 0.738 0.427

0

5

10

15

20

25

30

35

40


TotalLipid(%)

Conditions

Befor growth phase After growth phase End of starvation phase

0

2

4

6

8

10

12

14

16

NeutralLipid(%)

Befor growth phase After growth phase End of starvation phase

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Contro 5g 10g 15g 20g

Conditions

7/27/2019 12,12 First cycle (1).xls

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Ph

Day 11 Day 12 Day 13 Day14 Day15 Day 1 Day 2 Day 3

0.634 0.424 0.084 0.024 0.012 Control 8.3 8.6 8.43

0.183 0.093 0.046 0.028 0.013 5g 7.28 7.12 6.94

0.398 0.114 0.084 0.057 0.032 10g 7.37 7.21 7.14

0.385 0.117 0.073 0.064 0.047 15g 7.31 7.18 7.040.148 0.103 0.084 0.035 0.021 20g 7.27 7.14 6.93

Ph

Day 11 Day 12 Day 13 Day14 Day15 Day 1 Day 2 Day 3

0.639 0.428 0.089 0.027 0.011 Control 7.35 6.82 6.53

0.185 0.096 0.048 0.024 0.014 5g 7.28 7.12 6.94

0.385 0.118 0.082 0.051 0.038 10g 7.37 7.21 7.14

0.347 0.116 0.064 0.042 0.032 15g 7.31 7.18 7.04

0.137 0.105 0.084 0.031 0.026 20g 7.27 7.04 6.95

6

6.5

7

7.5

8

8.5

9

1 2 3 4 5 6 7 8 9

Ph

Days

Control 5g 10g 15g

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8.38 8.52 8.37 8.48 8.53 8.62 8.51 8.73 8.69

6.65 7.04 6.74 6.48 7.32 8.2 8.38 8.42 8.64

6.92 6.74 6.52 6.42 6.73 7.47 8.17 8.38 8.46

6.83 6.42 6.57 6.85 7.23 7.64 7.87 8.35 8.546.85 6.46 6.67 6.85 7.32 7.53 7.75 8.21 8.36


6.89 6.5 6.85 6.92 7.08 7.25 7.04 6.68 6.53

6.65 7.04 6.64 6.48 6.75 6.62 6.95 7.03 7.14

6.82 6.74 6.58 6.42 6.74 6.63 6.52 6.48 6.63

6.83 6.42 6.57 6.85 6.64 6.86 7.13 6.95 6.79

6.86 6.63 6.73 6.85 6.74 6.58 6.63 6.85 6.87

0 11 12 13 14

20g

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Chlora

Day 13 Day14 Day15 Day 1 Day 2 Day 3 Day 4 Day 5

8.78 8.72 8.64 Control 3.843 3.532 3.354 3.858 2.743

8.81 8.73 8.75 5g 2.753 2.532 2.582 2.592 2.214

8.58 8.74 8.65 10g 5.949 5.653 5.474 5.321 5.284

8.32 8.21 8.37 15g 6.482 6.385 6.175 5.369 5.0458.26 8.38 8.43 20g 4.402 3.843 3.349 3.62 3.349

Day 13 Day14 Day15 Day 1 Day 2 Day 3 Day 4 Day 5

6.44 6.57 6.88 Control 3.883 3.548 3.357 3.879 2.741

7.06 7.2 7.08 5g 2.763 2.538 2.585 2.589 2.223

6.45 6.72 6.83 10g 5.95 5.659 5.453 5.325 5.287

6.65 6.83 6.9 15g 6.487 6.754 6.182 5.374 5.047

6.92 6.74 6.78 20g 4.407 3.847 3.35 3.67 3.354

0

1

2

3

4

5

6

7

1 2 3 4 5 6 7 8

Chlor

ophylla(g/ml)

Days

Control 5g

1

2

3

4

5

6

7

8

Chlorophylla(g/ml)

Control 5g

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1 2 3 4 5 6 7 8 9 10

Days

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Day 6 Day 7 Day 8 Day 9 Day 10 Day 11 Day 12 Day 13 Day14

2.841 2.859 2.546 2.873 2.643 2.993 2.329 2.254 2.231

2.115 2.226 2.165 2.043 2.021 2.321 2.145 2.276 2.224

5.154 5.095 4.843 4.563 4.135 3.743 3.432 3.215 3.114

4.454 4.275 4.258 4.142 3.553 3.721 3.732 2.325 2.7953.316 3.273 2.943 2.43 2.221 2.173 2.17 2.163 2.121

Day 6 Day 7 Day 8 Day 9 Day 10 Day 11 Day 12 Day 13 Day14

2.854 2.864 2.532 2.879 2.656 2.954 2.335 2.258 2.238

2.119 2.239 2.169 2.049 2.029 2.328 2.153 2.28 2.228

5.163 5.102 4.856 4.569 4.139 3.753 3.438 3.221 3.116

4.464 4.278 4.263 4.147 3.558 3.728 3.739 2.322 2.799

3.319 3.275 2.948 2.45 2.229 2.183 2.17 2.168 2.132

9 10 11 12 13 14

10g 15g 20g

10g 15g 20g

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11 12 13 14

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Chlorb

Day15 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7

2.119 Control 3.819 3.521 3.345 3.821 2.721 2.832 2.845

2.143 5g 2.743 2.526 2.576 2.585 2.21 2.103 2.215

3.003 10g 5.934 5.643 5.456 5.313 5.276 5.134 5.06

2.276 15g 6.453 6.356 6.164 5.343 5.032 4.432 5.0751.743 20g 4.394 3.823 3.336 3.578 3.324 3.308 3.258

Chlorb

Day15 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7

2.125 Control 3.814 3.516 3.332 3.816 2.714 2.827 2.836

2.146 5g 2.741 2.518 2.565 2.546 2.21 2.096 2.211

3.012 10g 5.928 5.641 5.451 5.31 5.272 5.131 5.01

2.271 15g 6.421 6.351 6.154 5.338 5.027 4.427 5.064

1.747 20g 4.386 3.817 3.331 3.564 3.317 3.297 3.251

0

1

2

3

4

5

6

7

1 2 3 4 5 6 7 8 9 10 11 12 13 14

Chlorophyllb(g/ml)

Days


0

1

2

3

4

5

6

7

1 2 3 4 5 6 7 8 9 10 11 12 13 14

Chlorophyllb(g/ml)

Days


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Day 8 Day 9 Day 10 Day 11 Day 12 Day 13 Day14 Day15

2.538 2.87 2.64 2.983 2.321 2.245 2.212 2.104

2.148 2.012 2.008 2.314 2.123 2.256 2.213 2.132

4.464 4.556 4.123 3.734 3.421 3.184 3.104 3.004

4.832 4.553 3.534 3.721 3.732 2.314 2.753 2.2562.931 2.378 2.216 2.153 2.165 2.145 2.116 1.732

Day 8 Day 9 Day 10 Day 11 Day 12 Day 13 Day14 Day15

2.525 2.845 2.542 2.967 2.314 2.225 2.219 2.101

2.142 2.006 2.008 2.308 2.117 2.251 2.209 2.126

4.462 4.548 4.117 3.737 3.416 3.173 3.097 2.986

4.828 4.543 3.53 3.716 3.726 2.307 2.747 2.242

2.928 2.365 2.213 2.147 2.154 2.138 2.106 1.726

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Cell density

Days

Control (12/12) 5 g 10 g 15 g

0 1730150.5 1730150.5 1730150.5 1730150.5

22193882.2 2472839.2 2634492.4 1982302.3

4 2491838.8 2734349.3 2934382.3 2182394.9

6 2739393.3 3013824.9 3232043.4 2703843.5

8 2983992.2 3313293.3 3800734.9 2924830.1

10 2938381.0 3224090.3 3791991.2 2938430.4

12 2909128.6 3103923.5 3711913.5 2901939.3

14 2803289.3 3084239.5 3700482.3 2899372.4

Cell density

Days

Control (12/12) 5 g 10 g 15 g

0 1642180.5 1580520.8 1624030.1 1560566.1

2 2152482.2 2288239.2 2434152.3 1967311.5

4 2231528.8 2684449.3 2727381.2 2292144.3

6 2529416.9 3093827.3 3141083.3 2407122.5

8 2627992.6 3167476.3 3605729.2 2714157.1

10 2878381.7 3034240.3 3584891.7 2627113.4

12 2749578.4 2943304.9 3606424.7 2728452.1

14 2608219.0 3035279.5 3627452.1 2698471.4

0.00E+00

5.00E+05

1.00E+06

1.50E+06

2.00E+06

2.50E+06

3.00E+06

3.50E+06

4.00E+06

0 2 4 6 8 10 12 1

Cellden

sity(cells/ml)

Days

Control (12/12) 5 g 10 g 15 g

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1.50E+06

2.00E+06

2.50E+06

3.00E+06

3.50E+06

4.00E+06

0 2 4 6 8 10

CellDensity(cells/ml)

Days

Control (12/12) 5 g 10 g 15 g

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Initial COD Final COD COD r %

20 g

1730150.5 Control 300 38.3 87.23333

2398599.25 g 5012 900.3 82.03711

2620409.3 10 g 10078.8 1643.4 83.69449

2834020.5 15 g 15030.2 3194.3 78.74746

3000492.1 20 g 20040.2 5120.2 74.45035

2930349.4

2900340.5

2892494.4

20 g

1634470.6

2265261.2

2517293.3

2625219.5

2807432.2

2520372.1

2616240.6

2532274.1

0 g

65

70

75

80

85

90

Control 5 g 10 g 15

CODr(%)

Days

CODr

COD

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12 14

20 g

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Initial After growth End of stravation Phase

Control (12 38.3 320.3 122.3

5 g 38.3 374.4 132.710 g 38.3 396.3 102.5

15 g 38.3 365.3 112.6

20 g 38.3 360.4 121.6

g 20 g

r

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Biomass

Day 1 Day 2 Day 3 Day 4

Control 0.284 0.3 0.327 0.342

5 g 0.286 0.299 0.317 0.331

10 g 0.304 0.329 0.338 0.398

15 g 0.273 0.317 0.321 0.338

20 g 0.243 0.252 0.263 0.272

0

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0.45

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Biomass(g/l)

Control

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Day 5 Day 6 Day 7 Day 8 Day 9 Day 10 Day 11 Day 12 Day 13 Day 14

0.354 0.363 0.37 0.354 0.362 0.353 0.341 0.327 0.316 0.304

0.342 0.357 0.383 0.363 0.347 0.332 0.328 0.322 0.317 0.283

0.362 0.374 0.407 0.403 0.388 0.373 0.343 0.362 0.352 0.338

0.351 0.373 0.394 0.364 0.39 0.36 0.332 0.326 0.321 0.313

0.281 0.3 0.323 0.309 0.302 0.332 0.223 0.238 0.227 0.221

7 8 9 10 11 12 13 14 15Days

5 g 10 g 15 g 20 g

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Biomass


Control 0.273 0.299 0.302 0.314 0.322 0.328 0.332

5 g 0.282 0.295 0.312 0.33 0.348 0.352 0.354

10 g 0.307 0.318 0.324 0.331 0.356 0.363 0.361

15 g 0.275 0.285 0.29 0.298 0.304 0.313 0.32

20 g 0.262 0.268 0.274 0.278 0.285 0.295 0.305

0.25

0.27

0.29

0.31

0.33

0.35

0.37

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Biomass

(g/l)

Days

Control 5 g 10 g 1

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0.327 0.321 0.316 0.306 0.312 0.311 0.307

0.348 0.353 0.347 0.338 0.332 0.324 0.318

0.363 0.356 0.351 0.346 0.341 0.338 0.33

0.311 0.298 0.287 0.281 0.276 0.262 0.257

0.3 0.294 0.287 0.282 0.273 0.267 0.261

13 14

g 20 g

12,12 first cycle (1).xls

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