15. dr. patrick kobina arthur - university of ghana...microsoft powerpoint - 15. dr. patrick kobina...
TRANSCRIPT
![Page 1: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/1.jpg)
01/23
![Page 2: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/2.jpg)
Rustad et al, 200902/23
![Page 3: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/3.jpg)
03/23
![Page 4: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/4.jpg)
The birth of Antibiotics:
Pencillium notatum
04/23
![Page 5: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/5.jpg)
• Pleuromutilin produced by Clitopilus sp(Pleurotus sp )
• Active against gram positive bacteria• Binds to 23S rRNA
PleuromutilinRetapamulin
05/23
![Page 6: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/6.jpg)
06/23
![Page 7: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/7.jpg)
07/23
![Page 8: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/8.jpg)
08/23
![Page 9: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/9.jpg)
HIT-rate (WDF, n=189)>>366X~4
M. smegmatis : 78%S. aureus : 68% E. coli : 44% C. albicans : 31%
HIT-rate (MEF, n=29)>>(+143)light/dark
M. smegmatis : 98% / 78%S. aureus : 58% / 43% E. coli : 20% / 0%C. albicans : 13% / 0%
HIT-rate (ABF, n=111)>>800+
M. smegmatis : 87%S. aureus : 19% E. coli : 8% C. albicans : 30%
HIT-rate (TEF, n=0)>>400+
M. smegmatis : --S. aureus : --E. coli : --C. albicans : --
HIT-rate (SBF, n=0)>> 201
M. smegmatis : --S. aureus : --E. coli : --C. albicans : --
Total: 2700+09/23
![Page 10: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/10.jpg)
0
100
200
300
400
500
600
700
800
900
1000
AMP 40 AMX 40 VAN 40 INH10 EMB 20 PZD 40 MOXI 0.5 RIF 10 LIN 5 TET 20 CHLO 40 ERY 40 STREP 20
CYSERIN 2
Zone
of i
nhib
ition
(mm
) M. smegmatis (24 hrs) Phenotypic Array for Standard Antibiotics
0/22 1/22
18/22 15/22
22/22
1/22
22/22
3/22
22/22
22/22
21/22
22/22
22/22
0/22
10/23
![Page 11: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/11.jpg)
0
100
200
300
400
500
600
700
800
900
1000
AMP 40 AMX 40 VAN 40 INH10 EMB 20 PZD 40 MOXI 0.5 RIF 10 LIN 5 TET 20 CHLO 40 ERY 40 STREP 20
CYSERIN 2
Zone
of i
nhib
ition
(mm
) Ery-M. smegmatis (24 hrs) Phenotypic Array for Standard Antibiotics
18/22
12/22
1/22 0/22 0/22 0/22 1/22
22/22
0/22 0/22 0/22 1/22
17/22
0/22
11/23
![Page 12: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/12.jpg)
12
Reverse Phase C-18
Size Exclusion LH-20
Bioassay
12/23
![Page 13: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/13.jpg)
13
BCG cell (GFP)Drug treatment
Quantitative measurements of Cell viability and growth13/23
![Page 14: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/14.jpg)
Mass Spec1D Proton NMR13C, 15N,HSQC, HMBC, HMQC, COSY, TOCSY, ROESY, NOESYetc…14/23
![Page 15: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/15.jpg)
15/23
Miguel A. MatillaGeorge P. C. SalmondDepartment of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW
![Page 16: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/16.jpg)
16/23
![Page 17: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/17.jpg)
17/23
![Page 18: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/18.jpg)
18/23
![Page 19: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/19.jpg)
19/23
![Page 20: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/20.jpg)
20/23
![Page 21: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/21.jpg)
21/23
![Page 22: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/22.jpg)
22/23
![Page 23: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/23.jpg)
23/23
![Page 24: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/24.jpg)
Project Team MembersSamuel Yaw Aboagye (2009-2011)
Priscilla Koduah (2010-2011)
Vincent Amarh (2011-2012)
Clement Opoku-Temeng (2012-2013)
Samuel Mawuli Adadey (2012- )Gloria Baaba Arkaifie (2013- )Arnold Abraham (2013 - )Precious Cramer (2013 - )
Mentorship and Project SupportDr. Paul A. LarteyLaGray ChemicalCompany – NsawamGHANA
Collaborators
University of CambridgeDr. Finian Leeper
Imperial College LondonDr. Brian Robertson
ETH-Zurich Prof. Ruedi AebersoldOlga SchuberMichael Zimmermann
University of Maryland -CPProf. Herman Sintim
UG-NMIMRProf. Dorothy Yeboah-Manu
FundingDAAD-GermanyGrand Challenges-CanadaTWAS-ItalyIFS-SwedenEMBO/SNF-SwissGates Foundation/NMIMRCAPREx/Alborada
![Page 25: 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina Arthur Author ayeboah Created Date 20161004161918Z](https://reader034.vdocument.in/reader034/viewer/2022042406/5f1f89b727966b60c54eb7c2/html5/thumbnails/25.jpg)