1.leaf (or cell) expansion lower leaf area less pn effects of water deficit:
Post on 15-Jan-2016
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1. Leaf (or cell) expansion
lower leaf area less Pn
Effects of water deficit:
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Volume time = m (P – Y)
cell wall extensibility
yield threshhold ofcell wall
Growth rate =
Reduction in cell expansion:
Both m and Y are determined by chemical-mechanical properties of the cell wall
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m (P – Y)
(6 – 4) growth
(4 – 4) no growth
when (P – Y) approaches zero no growth
Even if P is restored, growth may still be inhibited.
Why?
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Both m and Y can be altered by metabolism, enzymatic activity leading to softening or stiffening of wall
Drought
m decreased
Y increased
(i.e., stiffercell walls)
… and the change is slow to be reversed
This slows or stops growth …
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Effect of water deficit on maize shoot development
H2O content: 100% 16% 4% 2%
w (bars) -0.3 -2.0 -8.1 -16
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What do you conclude?
@ same , leaf/stem growth inhibited to a greater degree than root growth
What do you hypothesize?
V/t = m(P – Y)
roots in dry soil have: higher m lower Y
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What’s your next hypothesis?
Some (unknown) biochemical-enzymatic factor acts to cleave bonds in the cell wall, loosening wall structure and permitting growth.
Sharp, Silk & Hsiao:
found XET (xyloglucanendotransglycosylase) induced by ABA
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divides again when cell regains ~ original volume
no stress
stress
At any given point in time, cells are smaller, but DW growth is virtually unchanged.
time
volume growth slower
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In non-stress conditions, rate of in growth is faster.
Therefore:
acquire & allocate C much faster
i.e., re-invest “capital” or not
Growth depends on growth that has already occurred (compound interest analogy).
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Effects of water deficit:
2. Reduction in photosynthesis
Stomatal closure – impedes influx of CO2
Effects on biochemical capacity for photosyn.
• electron transport• rubisco activity
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Effects of Water Deficit
3. Phloem translocation
phloem transport
photosynthesis
sink activity
governed by
Stress effects?decrease photosynthesis less exporteddecrease sink activity leaf expansion hard hit
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0
5
10
15
20
25
30
0
2
4
6
8
0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.020
30
40
50
60
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90
100
Reflects sink activity(impaired very early)
% 14C remainingin the source leafafter 24 h
Net CO2
assimilationmol m-2 s-1
14C translocationvelocity (cm h-1)
Leaf (- MPa)
Reflects phloem loadingand export
Not strongly inhibited until leaf falls lower than ~ -12 bars
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escape desiccation desiccation postponement tolerance (maintain high ) (tolerate low )
H20 savers H2O spenders
osmotic adjustment
protoplasmicresistance
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4. Osmotic adjustment (a mechanism of tolerance of low )
An increase in the solute concentration of cell sap resulting from more solute molecules per cell rather than from a lower cell volume.
Effects of water deficit:
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a process by which can be decreased without an accompanying decrease in turgor
net increase in solute content per cell
do not confuse with increase in solute [ ] that occurs as a result of dehydration and shrinkage
Osmotic adjustment
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leaf cell = - 8 bars
xylem = - 6 bars
soil = - 1 bar
w = + p
-8 = -12 + 4
water flows in
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cell = - 15 bars (or water will flow out)
xylem = - 14 bars
soil = - 2 bar
w = + p
-15 = -12 + -3
consequence?
p decreases
(P – Y) zero
growth stops
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If you were a bio-mechanical engineer:
design this plant so that it cope with this situation.
what would you modify or enhance?