Cell and Tissue Culture

T.C.

Refers to technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium In vitro

History Haberlandt Realized that asepsis was necessary when culture

media enriched with organic substances metabolized by microorganisms

In 1898 he cultured single cells on Knop’s salt solution

In his cultures the cell were able to synthesize starch as well as increase in size

He failed in his goal to induce cell division

HistoryDespite of failures Haberlandt proposed that plant cells

are totipotent

Totipotency is ability of individual cells to express the phenotype of whole plant from which it is derived

Haberlandt is regarded as father of plant tissue culture

HistoryHanning(1904) Embryo culture of selected

crucifers

Robbins (1922), Kotte In Vitro culture of root tips

Laibach (1925) Use of embryo culture technique in inter-specific crosses of Linum

HistoryWhite (1934-37) Successful culture of tomato roots

importance of vitamins in growth media

Snow, Gautheret (1934-1939) Importance of auxin as growth regulator

Gautheret, Nobecourt, White (1939) Successful establishment of continuously growing cultures.

HistoryVan Overbeek (1941) Use of coconut milk

containing a cell division factor first time in Datura

Skoog and Tsui (1951), Miller et al (1955), In vitro cell differentiation, discovery of Kinetin

Morel and Martin (1952) Application of micro grafting to obtain virus free plants

HistoryMuir (1953) Isolation and culture of single cells

from plants

Skoog and Miller (1957) Discovery of principle of hormonal control of the organ formation in tissue culture

HistoryReinert and Steward (1958-59) First report on

somatic embryogenesis

Jones et al (1960) Hanging drop method of cell culture

Bergmann (1960) Bergmann cell culture technique

HistoryE C Cocking (1960) Isolation of protoplasts by

enzymatic degradation method

Murashige and Skoog (1962) Development of MS media

Guha and Maheshwawari (1964) Production of First haploid plant by anther culture

HistoryPower et al (1970) Protoplast fusion

Takabe et al (1971)Rgeneration of first plant from protoplast

Carlson et al (1972) First report on inter-specific hybridization through protoplast fusion