TLC analysis system

PVA-Alg system

• PVA-Alg immobilization 48h TLC analysis

48

h

0 h

Biodiesel fuel from Jatropha oil via non-catalytic supercritical methanol transesterification

Comparison

Biocatalysis Base Catalytic

Comparison 2

Bio

catalyst

Base

catalyst

Different reaction time for the conversion rate

24h 48h

FAME

Peanut and Soybean oil

Iodine vapor Diluted sulfuric acid carbonization

Soyb

ean

Peanu

t

Conclusion

• 1.預期Serraia M 產的Lipase有高度選擇性，從上圖可以看岀有中間產物無法催化成

FAME(fatty acid methyl ester)，坦若使用Pseudomous 進行催化則預測無此產生

• 2.鹼催化不殘留中間產物，反應完全高

• 3.TLC無法分辨大豆油和花生油差別

• 4.TLC可快速用於分析生質柴油是否產生

Alginate 3% immobilization System

3% 5% 7% 10% 13% 15%(Oil: Catalyst weight %)

• Alginate immobilization system

• 看不同固定化重對大豆油轉化率影響

• (算法= 菌種:大豆油重)

• 條件=甲醇:油 mol=1.3:1

• 溫度30℃

• 150RPM

• 時間1~2days

3% 5% 7% 10% 13% 15%

24h reaction

2 repeat

Serratia lipase features

Alginate system 24H

FAME

48h figure

• 今天下午四點驗收

FAME定量

• HPLC and GC

• 1.內標法:和樣品一起打入分析(標準品濃度已知)

• 2.外標法:用已知的FAME作標準曲線

內標法internal standard

• METHYL HEPTADECANOATE STANDARD FOR GC

• 十七烷酸甲酯 1G =3850NTD

外標法

• methyl palmitate 34.80USD

• methyl arachidate 78.30USD

• methyl stearate 35.90USD

• methyl linolenate (100MG) 40.60USD

• methyl linoleate 37.30USD

• methyl oleate 35.90USD

• Total =262.8USD= 8409.6NTD

外標法(用於棕梠油)

• Methyl oleate 35.90USD•

• methyl palmitate 34.80USD•

• methyl stearate 35.90USD•

• methyl linoleate 37.30USD•

• methyl myristate 45.00USD • total least: 188.9~~~6076.8NTD

Soybean oil

Project

• TLC系統(V)

• HPLC第二次分析(X)

• NEXT:

• 要使用哪個系統定FAME(biodiesel)量

• 比較固定化系統轉酯率

• 使用Pseudomous作轉酯化