2 5 data
TRANSCRIPT
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TLC analysis system
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PVA-Alg system
• PVA-Alg immobilization 48h TLC analysis
48
h
0 h
Biodiesel fuel from Jatropha oil via non-catalytic supercritical methanol transesterification
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Comparison
Biocatalysis Base Catalytic
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Comparison 2
Bio
catalyst
Base
catalyst
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Different reaction time for the conversion rate
24h 48h
FAME
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Peanut and Soybean oil
Iodine vapor Diluted sulfuric acid carbonization
Soyb
ean
Peanu
t
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Conclusion
• 1.預期Serraia M 產的Lipase有高度選擇性,從上圖可以看岀有中間產物無法催化成
FAME(fatty acid methyl ester),坦若使用Pseudomous 進行催化則預測無此產生
• 2.鹼催化不殘留中間產物,反應完全高
• 3.TLC無法分辨大豆油和花生油差別
• 4.TLC可快速用於分析生質柴油是否產生
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Alginate 3% immobilization System
3% 5% 7% 10% 13% 15%(Oil: Catalyst weight %)
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• Alginate immobilization system
• 看不同固定化重對大豆油轉化率影響
• (算法= 菌種:大豆油重)
• 條件=甲醇:油 mol=1.3:1
• 溫度30℃
• 150RPM
• 時間1~2days
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3% 5% 7% 10% 13% 15%
24h reaction
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2 repeat
Serratia lipase features
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Alginate system 24H
FAME
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48h figure
• 今天下午四點驗收
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FAME定量
• HPLC and GC
• 1.內標法:和樣品一起打入分析(標準品濃度已知)
• 2.外標法:用已知的FAME作標準曲線
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內標法internal standard
• METHYL HEPTADECANOATE STANDARD FOR GC
• 十七烷酸甲酯 1G =3850NTD
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外標法
• methyl palmitate 34.80USD
• methyl arachidate 78.30USD
• methyl stearate 35.90USD
• methyl linolenate (100MG) 40.60USD
• methyl linoleate 37.30USD
• methyl oleate 35.90USD
• Total =262.8USD= 8409.6NTD
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外標法(用於棕梠油)
• Methyl oleate 35.90USD•
• methyl palmitate 34.80USD•
• methyl stearate 35.90USD•
• methyl linoleate 37.30USD•
• methyl myristate 45.00USD • total least: 188.9~~~6076.8NTD
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Soybean oil
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Project
• TLC系統(V)
• HPLC第二次分析(X)
• NEXT:
• 要使用哪個系統定FAME(biodiesel)量
• 比較固定化系統轉酯率
• 使用Pseudomous作轉酯化