General Session 1: Microtomy: The Melding of Art & Science. Presented by Jan Minshew, HT/HTL(ASCP), Field Product Manager-

Sectioning, Leica Biosystems

A good microtome and a sharp blade are important, but there is an ingenious combination of techniques, knowledge and skill necessary to produce good sections. Let’s spend some time together minimizing the frustration you might feel when you sit down at the microtome or review your work under the microscope.

General Session 2: Breast Cancer - Before & After. Presented by Benjamin Calhoun, MD, PhD, Surgical Pathology, Carolinas Medical Center

The theme of the talk is before and after – touching on what happens to breast specimens before they reach the Histology lab (and how that might impact the lab) and then showing examples of the immunohistochemistry that we often use after the slides are received by a pathologist (and how that might impact the lab and the patient).

General Session 3: Why the “e” in ePathology is so Exciting for Histology’s Future. Presented by Jared Schwartz, M.D., Ph.D., Chief Medical Officer, LBS and Consulting Professor of Pathology, Stanford University Medical Center

This session will define ePathology and how it is transforming histology. Dr. Schwartz will discuss how histology staff plays a critical role in the introduction and use of new imaging technologies and explore how ePathology and imaging improve all aspects of a QA program.

General Session 4: Automated Tracking of Cases in Surgical Pathology. Presented by Monica Calhoun, Carolinas Pathology Group

This session will give the audience a first hand account of implementing and using and automated barcode tracking system in in a high volume Surgical Pathology department.

General Session 5: Using Lean Concepts to Transform Your Working Environment. Presented by Angela Smith, HT(ASCP), Carolinas Medical

Center & Nate Bergh, MBA, Carolinas Healthcare System

Overview of Lean concepts: Huddles, 8 waste, 5’S and Problem solving. The group activities will include problem solving real histology issues. This session will also include examples in Histology with proven success.

Workshop A: Immuno-cyto/Histo-chemical Detection Methods and Reagents. Presented by Joe Myers,CT(ASCP)QIHC, Biocare Medical

This workshop is intended to provide participants with a review of the scientific development of IHC detection methods, with emphasis on the most commonly employed procedures and reagents. Both immuno-fluorescence and immuno-enzymatic procedures will be discussed, as well as the advantages and disadvantages of various approaches – e.g. increased ‘signal’ detection with polymer-based systems and increased ‘noise’ detection with biotin/avidin-based systems.

Workshop B: Interpretation of Special Stains. Presented by M. Lamar Jones, HT(ASCP) Carolinas School of Health Sciences

This workshop will provide the participant with a variety of special stains and their use in histotechnology and pathology. The mechanisms of staining will be presented to include fixation, controls, purpose, staining characteristics and special techniques. Pathology case studies will be presented to demonstrate application of many common special stain techniques and the purpose for requesting the stain(s).

Workshop C: Immunofluorescence. Presented by Gayle Callis HT(ASCP)HTL, MT(ASCP), GCallis Histology Service LLC

Immunofluorescence has been done in clinical laboratories for many years for detection of immune deposits in kidney or skin biopsies and some infectious diseases. Although IF staining is often a preferred method in research laboratories, it can be just as useful for clinical diagnosis especially when double IF provides distinct, clear co-localization of cells or tissue components as compared to chromogenic IHC. IF can be used with either FFPE (formalin fixed paraffin embedded) or frozen sections. Tissue preparation, fixation, reagents, fluorophores, nuclear counterstains, mounting medias along with technical hints to achieve good staining are presented. One can use non-kit IF methods, recently available commercial IF kits or manipulate/modify an existing enzyme immunohistochemistry (IHC) kits to work for IF staining. Rationale for using double IF is presented along with defining some basic fluorescence terminology, citing and ways to avoid or solve problems e.g. fluorescence quenching, photo-bleaching and aldehyde induced or naturally occurring auto-fluorescence.

Register today for the first ever 2 Day Carolina Symposium sponsored by the National Society for Histotechnology, the North Carolina Society of Histotechnology and the South Carolina Society of Histotechnology. This two day collaboration brings the best of the three organizations offering a fantastic educational program and an Exhibit Fair for one low price! Attendees have the opportunity to earn up to 10.5 continuing education credits.

Carolina Symposium2 Da

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>PROGRAM DESCRIPTIONS<

Workshop D: Fixation & Processing. Presented by Jan Minshew, HT/HTL(ASCP), Field Product Manager-Sectioning, Leica Biosystems

The purpose of this presentation is to explain the science involved in fixation and processing in an easy to comprehend manner. Fixation is the most important step in producing high quality slides, but the process is often rushed in the interest of decreasing turn-around time. Each step in processing also has great importance, but the science is sometimes poorly understood. The main goal of this workshop is to help participants understand and appreciate the effect and importance of each step during fixation and tissue processing. Participants will leave with the skills necessary to obtain consistent, high quality specimens and troubleshoot easily if problems arise. With this knowledge they will be able to eliminate crunchy biopsies and the need to reprocess large fatty tissue. Emphasis will be placed on formalin fixation and routine and rapid paraffin processing, since they are the most common.

Workshop E: Hints to HistoHappiness. Presented by Linda Jenkins,HT(ASCP), Clemson University & Gayle Callis,HT(ASCP)HTL,

MT(ASCP), GCallis Histology Service LLC

Have you ever been frustrated by bubbles in a water bath? What about poor ribbon formation during sectioning? Ever plugged up a processor? Can’t flatten a curled section? Do you have trouble with eosin bleeding or fuzzy looking tissue sections after cover slipping? This workshop presents practical hints not commonly found in histotechnology textbooks in order to answer these questions plus many more in order to achieve optimal results in our daily work e.g. processing, sectioning, staining, etc. These are little things the two presenters have learned over the more than 90 year’s combined experience in research and clinical histopathology laboratories. Many hints are passed on by word of mouth or learned by trial and error and are the often fine, unwritten details that make a daily work routine in a histology laboratory run smoothly. Some of the hints are very simple, often amusing in nature that leave one asking “Why didn’t I try that?”. This will be a very interactive workshop where attendees are encouraged to discuss their favorite hints as we present the very practical useful side of histotechnology. Be prepared for a lively, fun time with Linda and Gayle.

Workshop F: Immunohistochemistry Applications in Clinical Dermatopathology. Presented by Joe Myers, CT(ASCP)QIHC, Biocare

Medical & Kelly Boyd, HTL(ASCP)QIHC, Provia Diagnostics, Inc

This presentation is intended to provide participants with a comprehensive overview of the provision of immunohistochemical staining services in a clinical dermatopathology laboratory setting, from the perspective of an experienced lab manager and IHC reagent/instrument specialist . It will cover many of the important issues involved in the provision of IHC in a ‘derm-path’ lab, including the handling of frozen and paraffin-embedded specimens, common antibody ‘menus’, and manual and automated staining procedures, as well as satisfying dermatopathologist’s expectations, reagent/instrument selection, procedure validation and quality control, and the delivery of cost-effective services. This seminar is intended to create awareness among fellow histology laboratorians who work in other settings of the unique aspects of performing IHC procedures within the ‘derm-path’ lab, and provide them with an opportunity to share their own experiences.

Workshop G: Are you ready for an CAP Accrediting Inspection : Update 2013. Presented by Elizabeth Sheppard, HT(ASCP), Ventana

Medical Systems

Preparing for an inspection can be an overwhelming task if you do not have the right tools and preparedness well before the day of the event. This lecture will provide participants information on how to prepare for an inspection. It will include information on quality control and quality assurance application to Pathology. Documentation and relative forms will be discussed. Review of all new 2013 checklist questions will be reviewed.

Workshop H: Today’s Artifacts – Tomorrow’s Facts? Presented by M. Lamar Jones, Carolinas College of Health Sciences

Is it real? How do I correct this problem? This workshop will demonstrate artifacts from many facets of Histotechnology such as fixation, tissue processing, embedding, microtomy, staining, IHC, coverslipping. The cause and correction of the artifact will be discussed.

Hotel Information: Hilton Charlotte Executive Park 5624 Westpark Dr. | Charlotte, NC 28217

Registrants are responsible for hotel / travel arrangements. We have secured a small block of rooms at the discounted group rate of $124.00 plus applicable taxes. To make reservations call 1-800-HILTONS and inform the agent that you are with the NSH Carolina Symposium. Hotel Deadline is March 12, 2013. All rooms provide complimentary high speed wifi.

Board of Certification HT Readiness Course presented by Robert Lott, HTL(ASCP)This workshop is designed to help the candidates study for the Histotechnician (HT) certification exam. An overview of the Computer Adaptive Testing (CAT) exam process and a review of the subject matter and study material useful for the exam will be presented. A study outline, which uses subject matter criteria published by the Board of Certification, as well as study hints will be shared. Students in a distance learning program, in a self-study situation, and those preparing for the Histotechnologist (HTL) exam will find this workshop useful. Extensive handout material covering all aspects of the process will be available for attendees.

Getting to the Meeting

The Hilton Charlotte Executive Park is located in the heart of Westpark Corporate Center, and only 5 miles from Downtown Charlotte. For those driving free self-parking is available at the hotel. If you are flying to Charlotte the hotel is only 5 miles away and a 15 minute drive from Charlotte Douglas International Airport (CLT). The airport offers a free airport shuttle, or they have taxi’s available and the average cost is $20.00 to the hotel.

>Travel Arrangements<

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Thursday, April 45pm – 8pm Check In

Friday April 57:30am Registration & Continental Breakfast8:30am – 10:00am General Sessions 1 & 2

Microtomy: The Melding of Art & Science Breast Cancer: Before & After

10:00am – 10:30am Refreshment Break 10:30am – 12:00pm Workshops A&B12:00pm – 1:15pm Grab & Go Lunches – Region III Meeting followed

by NC/SC Society Business Meetings1:15pm – 3:45pm Exhibit Fair Open1:45pm – 3:15pm General Sessions 3&4

Why the “e” in ePathology is so Exciting Automated Tracking of Cases in Surgical Pathology

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Membership Status (check all that apply):To qualify for member registration rates you must be a member of NSH and Your State Society

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3:15pm – 3:45pm Refreshment Break3:45pm – 5:15pm Workshops C&D5:15pm – 6:15pm Networking Reception in Exhibit Hall

Saturday, April 68:00am Continental Breakfast8:30am – 10:00am Workshops E & F8:30am – 4:15pm Board of Certification HT(ASCP)

Readiness Course (prereg required)10:00am – 11:30am Exhibit Fair Open11:30am – 1:00pm Award Luncheon in Exhibit Hall1:00pm – 2:30pm General Session 5

Using Lean Concepts to Transform Your Working Environment

2:30pm – 2:45pm Refreshment Break2:45pm – 4:15pm Workshops G & H

>Schedule at a Glance<

>Registration Form<

Select Registration Type:Full Symposium (both days)

Member ($225.00) Non Member ($299.00)

Student Member ($169.00)

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One Day Pass (one day)

Member ($139.00) Non Member ($259.00)

HT Readiness Course Only (Saturday, April 6)

HT Readiness Course Only ($139.00)

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Send in Your Registration! Fax: (443) 535-4055 | Email: histo@nsh.org | Via Mail: NSH, 8850 Stanford Blvd, Suite 2900, Columbia, MD 21045

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2 Day Summer SymposiumApril 5 - 6, 2013 | Hilton Charlotte Executive Park, Charlotte, NC Earn up 10.5 hours of continuing education credit!

8850 Stanford Blvd. Suite 2900Columbia, MD 21045PH. 443-535-4060 | www.nsh.org

Carolina Symposium 2Day

Histotechs/Cytotechs in Clinical, Veterinary & Research Settings Lab Supervisors & Managers Anyone involved in the collection, processing & evaluation of tissue samples

Who Should Attend?

Date: April 5-6, 2013

Place: Hilton Charlotte Executive Park,

Charlotte, NC

HT Eligible? Don’t Miss the Full Day HT Readiness Course on April 6th!