2 should we go with the flow - blood · microsoft powerpoint - 2 should we go with the flow.pptx...
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Should we Go With The Flow
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Fetomaternal Haemorrhage
• Kleihauer-Betke test used world wide as an inexpensive, simple & rapid method to determine FMH
• In Rh(D) Negative women post partum• In both Rh(D) Negative & Positive women
antepartum in cases of trauma or APH
• Suggested that Flow Cytometry more accurate as Kleihauer has a tendency to over estimate.
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Kleihauer Principle
• Fetal & maternal cells differentiated by acid elution & counter stained with eosin
• Counted using under microscope using a Miller occular
• Fetal Haemoglobin (HbF) – more resistant to denaturisation than adult haemoglobin (HbA)
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Kleihauer Slide
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Problems
• If mother has high level of HbF due to thalassaemia, sickle cell or hereditary persistence of fetal haemoglobin (HPFH) –intermediate staining cells
• Meticulous attention to performance & interpretation of test – otherwise accuracy is not guaranteed
• Manual test – variability in staining technique & counting
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Our Procedure
• Perform a Kleihauer as quick results in case of antepartum event
• If a large bleed detected (> 3 ml) allocate RhIg(if Rh Neg mother) on the results of the Kleihauer but send the sample for Flow Cytometry
• If the mother has Thalassaemia or high HbFlevels the Kleihauer is not valid
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Flow Cytometry
• 2 antibodies:
• One directed against fetal Haemoglobin (HbF)
• The other directed against Carbonic Anhydrase (CA), an enzyme only present in adult RBCs & very late stage fetal cells
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Case Study
• 35 yo G2P1, Rh Negative – low speed MVA at 33/40 on 7/08/18
• Presented to GP with lower abdo pain• Kleihauer requested – positive, sent for Flow
Cytometry (FC)• Rh(D) immunoglobulin issued – 2 x 625 IU• Follow up 48 hrs later – Klei & antibody screen• Mother on Thyroxine, anticoagulant, steriods
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TimelineDATE ABSCREEN KLEI FLOW RhIg6/07/18 Neg 1 x 625 IU *7/08/18 6.72 ml 10.32 ml8/08/18 Anti-D 7.92 ml 2 x 625 IU10/08/18 Anti-D 24.24 ml ?sample integrity 4 x 625 IU12/08/18 Anti-D 18.48 ml ?sample integrity 5 x 625 IU17/08/18 Anti-D 40.8 ml 55.0 ml **18/08/18 3 x 1500IU19/08/18 1 x 1500 IU21/08/18 Anti-D + Anti-C 32.9 ml 76.86 ml 5 x 1500 IU
• 28/40 prophylaxis
• ** Austin 34.3 ml
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7/08/18 12/08/18 17/08/18
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• Despite consistently high results from both Kleihauer & FC – no signs of fetal distress
• Haem Reg consulted with both patient & treating obstetric team
• Almost 3/52 post MVA caesarean performed at 36/40
• Male baby 2550g delivered with good APGARS• Hypoglycemic but no other issues. No FBE
taken.
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Questions
• Why were the results increasing – was the baby Rh(D) Negative or was it continuing to bleed.
• Why were the FC & Kleihauer results so discrepant
• If the last FC was correct would the baby not be in some distress
• CTG Tracing on 7th, 10th, 12th, 17th, 18th & 23rd
Aug