2004 dna barcoding all sections
TRANSCRIPT
![Page 1: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/1.jpg)
![Page 2: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/2.jpg)
INTRODUCTION
![Page 3: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/3.jpg)
Simple & Ambitious!
Advocates Opposition
ID all species
Discover newspecies
Speed up ID’s
Revitalize biological collections
Won’t work
Destroy traditionalsystematics
Service industry
Pseudo taxonomy
![Page 4: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/4.jpg)
New Scientist26 June, 2004
![Page 5: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/5.jpg)
Trends in Ecology& EvolutionFeb. 2003. Vol. 18, Iss. 2
3 of these 5 are the mostdownloaded papers in TREE
1. Taxonomy: renaissance or Tower of Babel? Jim Mallet et al4. A plea for DNA taxonomy Tautz et al5. The encyclopedia of life Edward Wilson
![Page 6: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/6.jpg)
Propositions to vote on:
i) Should we devote resources towards sequencing areference collection of specimens for the development aDNA barcoding system? [Yes, No, or Abstain]
ii) Should DNA sequences play a primary role in thediscovery of new species?
[Yes, No, or Abstain]
Vote before and after the debate!
![Page 7: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/7.jpg)
![Page 8: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/8.jpg)
SPECIMENS
![Page 9: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/9.jpg)
Barcoding Specimens1 2
3
4
5
![Page 10: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/10.jpg)
Specimen 1
1
Class Bivalvia Linnaeus, 1758 -- bivalves Order Unionoida Stoliczka, 1871 Family Unionidae Fleming, 1828 Genus Epioblasma Rafinesque, 1831 Species Epioblasma torulosa (Rafinesque, 1820)
tubercled blossom
Wabash County, Illinois
EXTINCT
![Page 11: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/11.jpg)
Specimen 22
Class Insecta -- insects Order Coleoptera -- beetles Family Scarabaeidae -- scarab beetle Genus Melolontha Species Melolontha melolontha (L., 1758)
common cockchafer, May bug
Hungary
Very distinctive, but European
![Page 12: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/12.jpg)
Specimen 3
3
Class Clitellata/Hirudinea Lamarck, 1818 -- leeches Order Arhynchobdellida Blanchard, 1894 Family Erpobdellidae Blanchard, 1894 Genus Erpobdella de Blainville, 1818 Species Erpobdella punctata (Leidy, 1870)
Leech (no common name)
Illinois
Common & widespread in NANot very distinctive
![Page 13: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/13.jpg)
Specimen 4
New species, no PEET program!
Psocid (no common name)
Arizona
Class Insecta -- insects Order Psocoptera -- psocids Family Myopsocidae Genus Myopsocus Species Myopsocus sp. n.
4
![Page 14: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/14.jpg)
Specimen 5
Fly Agaric
Illinois
Class Homobasidiomycetae Order Agaricales -- stereotypical mushrooms Family Amanitaceae Genus Amanita Species Amanita muscaria var. formosa
5
e.g. of CONTAMINATION!Vouchering essential, No COI
![Page 15: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/15.jpg)
Conclusions
• Some specimens unsuitable for barcodinge.g. Fossilized/Extinct taxa
• No COI for many taxa• Many taxa difficult/impossible to ID with
e.g. immature specimens, cryptic taxa etcmorphology• No specialists for many taxa• No training necessary for barcoding!• Already in use for many taxa ( bacteria, fungi,
Cetacea)
![Page 16: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/16.jpg)
![Page 17: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/17.jpg)
DEBATEQUESTIONS
![Page 18: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/18.jpg)
A) What is DNA Barcodingi) What are the barcoding regions and what are theirproperties that make them useful?
[Richard Pyle, Bishop Museum, USA – amongst others]
![Page 19: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/19.jpg)
A) What is DNA Barcodingii) Would the DNA barcode represent a *definition* of aspecies?
[David Fitch, New York University, USA – amongst others]
![Page 20: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/20.jpg)
A) What is DNA Barcodingiii) How would DNA barcoding work in practice and whoshould be doing it?
[Vince Smith, Illinois Natural History Survey, USA]
![Page 21: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/21.jpg)
B) DNA barcoding in species identification
i) How will the DNA barcoding identifications deal withthe overlap between intraspecific and interspecificvariation documented in many groups? [Everybody!]
![Page 22: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/22.jpg)
B) DNA barcoding in species identification
ii) To what extent will DNA barcoding support the effortsof traditional taxonomy?
[David Yeates, CSIRO, Australia]
![Page 23: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/23.jpg)
B) DNA barcoding in species identificationiii) Is accuracy of identification (to the right clade)possible in the absence of accuracy in family- and genus-level topology? [Jim Hayden, Cornell University, USA]
![Page 24: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/24.jpg)
C) DNA barcoding in species discoveryi) How confident can we be in the utility of DNA barcodesto discover new species when it has been demonstrated thatmany species are not mitochondrially monophyletic, andthus share mitochondrial polymorphisms with other species?
[Daniel Funk, Vanderbilt University, USA]
![Page 25: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/25.jpg)
C) DNA barcoding in species discoveryii) With a program of DNA barcoding what would besufficient to demonstrate that a specimen represents a newspecies? [Kevin Johnson, Illinois Natural History Survey, USA]
![Page 26: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/26.jpg)
D) Political & practical implicationsi) What will be the role of systematists in a world wheremost identifications are done by "barcode" and will theexpansion of sequencing efforts come at the expense ofsystematics in general [Jim Hayden, Cornell University, USA]
![Page 27: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/27.jpg)
D) Political & practical implicationsii) Assuming the technical problems of DNA barcodingcan be overcome is it now, or will it ever be cost-effectiverelative to traditional methods to use DNA barcodes forbioinventory purposes? [Derek S. Sikes, University of Calgary, Canada]
![Page 28: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/28.jpg)
D) Political & practical implicationsiii) Will identification by barcodes increase people'senthusiasm for living things?
[Jim Hayden, Cornell University, USA]
![Page 29: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/29.jpg)
![Page 30: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/30.jpg)
CLOSINGREMARKS
![Page 31: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/31.jpg)
Propositions to vote on:
i) Should we devote resources towards sequencing areference collection of specimens for the development aDNA barcoding system? [Yes, No, or Abstain]
ii) Should DNA sequences play a primary role in thediscovery of new species?
[Yes, No, or Abstain]
![Page 32: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/32.jpg)
Lessons for the all species project, from thehuman genome project…
Kevin Kelly
• The Human Genome Project featured a goal that probably would havehappened anyway over time… It took ordinary work and raised it tothe level of legend and myth by attempting to complete it "all" in arelatively short time. The Genome project then is primarilydistinguished by its emphasis on "all."
![Page 33: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/33.jpg)
Lessons for the all species project, from thehuman genome project…
Kevin Kelly
• A reoccurring theme in the mission statement of the All SpeciesInventory is the need for radically different and new tools. All Speciesmust be open to the possibility of succeeding using enhanced existingtools applied in new ways, or simply old tools automated to lighteningspeed. However because current taxonomic procedures are so low tech,almost any improvement may resemble radical technology.
![Page 34: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/34.jpg)
Lessons for the all species project, from thehuman genome project…
Kevin Kelly
• All Species has an even better chance to become a projectthe public cares about. Far more people can identify aknown species than can identify a known DNA sequence…This is a project that can relate to everyone: All species forall people.
• Someday all the species living on earth will be identified,although surely not all the ones alive today. Why not now?
![Page 35: 2004 Dna Barcoding All Sections](https://reader031.vdocument.in/reader031/viewer/2022031903/55a543a61a28abba478b463e/html5/thumbnails/35.jpg)
Acknowledgements:
The Panel: Paul Hebert & Kip Will
The Students: Martin Hauser, Daniela Takiya, MathysMeyer, Floyd Shockley, & Jamie Zahniser
Specimen providers: Kevin Cummings, Martin Hauser,Andrew Miller, Mark Wetzle, & Kazunori Yoshizawa
Conference organisers: Especially Mike Irwin & GailKampmeier
Funding: National Science Foundation