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20202017 alexmk2 quick guide a4...alex2 stop solution 8 min 100 µl 400 µl 100 µl 500 µl 500 µl...

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  • 20202017 ALEXMK2 Quick Guide A4...ALEX2 Stop Solution 8 Min 100 µL 400 µL 100 µL 500 µL 500 µL 500 µL 500 µL 500 µL Title 20202017_ALEXMK2_Quick_Guide_A4 Created Date 2/17/2020
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1 3 5 Min x3 5 5 Min x5 2 2h 4 30 Min 6 7 30 Sec 45 Min 8 Quick Guide ALEX 2 Washing Solution ALEX 2 Sample Diluent Patient Sample ALEX 2 Detection Antibody ALEX 2 Substrate Solution ALEX 2 Stop Solution 8 Min 100 μL 400 μL 100 μL 500 μL 500 μL 500 μL 500 μL 500 μL

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Page 1: 20202017 ALEXMK2 Quick Guide A4...ALEX2 Stop Solution 8 Min 100 µL 400 µL 100 µL 500 µL 500 µL 500 µL 500 µL 500 µL Title 20202017_ALEXMK2_Quick_Guide_A4 Created Date 2/17/2020

1 3

5 Min x3

5

5 Min x5

2

2h

4

30 Min

6 7

30 Sec

45 Min

8

Quick Guide

ALEX2 Washing Solution

ALEX2 Sample Diluent

Patient Sample

ALEX2 Detection Antibody

ALEX2 Substrate Solution

ALEX2 Stop Solution

8 Min

100 µL

400 µL

100 µL

500 µL 500 µL 500 µL 500 µL 500 µL

MultiPurpose Sampler MPS MPS robotic MPS liquid · MultiPurpose Sampler MPS MPS robotic MPS roboticpro MPS liquid. 2 ... 50 µL 100 µL 250 µL 500 µL ... 500 l 1100111-805-00

Automated Plasma Proteomic Sample Preparation · tube of digest, and shake (RT; 500 rpm; 10 minutes) 5. Add 120 µL of digest to rows 1–6 to an MTP & DIGEST 6. Add 120 µL of stop

pET Express & Purify Kits User Manual - Takara Bio Manual/PT5018-1.pdf15 µl pET6xHN-C Vector (In-Fusion Ready) [100 ng/µl] 10 µl pET6xHN-GFPuv Vector [500 ng/µl] 15 µl 1.1 kb

Minicircle DNA Technology · 1 µl 10x T4 DNA ligase buffer 6 µl deionized water 1 µl T4 DNA ligase (40 U/ µl) 10 µl Total Volume b. Incubate the ligation reaction at 16°C overnight

AUTOMATED LIQUID HANDLING · MICROLAB M Volume 10 µL 25 µL 50 µL 100 µL 250 µL 500 µL 1 mL 2.5 mL 5 mL 10 mL 25 mL Model 1701 1702 1705 1710 1725 1750 1001 1002 1005 1010 1025

Verification Procedure for Accuracy and Precision · P10, P10N, U10 1.0 µL to 10 µL D10, DL10 DF10, DFL10 C10, C8x10, C1 x10 0. to 10 µL P 0, P 0N, U 0 µL to 0 µL U8x 0, U1 x

Ultrafiltration Products - Wolfson Centre Home Pagewolfson.huji.ac.il/purification/PDF/dialysis/VIVASPIN...100 µl to 600 µl samples Vivaspin 500 µl centrifugal filter units offer

Supplementary Materials for · 2020. 3. 4. · 2 At this point plates were pooled in a single 500 µl Eppendorf tube and treated with 1 µl of Exonuclease I (Thermo) for 30 min at

ab136933 Aldosterone ELISA Kit - abcam.com · 500 250 125 3 500 µL Standard #2 500 125 62.5 4 500 µL Standard #3 500 62.5 31.3 5 500 µL Standard #4 500 31.3 15.6 6 500 µL Standard

SofTouch...8707-06XX 8-Channel 5 - 100 µl1 µl 200 µl, 300 µl 8707-07XX 8-Channel 25 - 250 µl5 µl 300 µl 8707-08XX 8-Channel 50 - 1200 µl 10 µl 1200 µl 8707-09XX 12-Channel

Thermo Scientific molecular biology workflow solutionsDreamTaq Green DNA Polymerase 500 U/2,500 U FEREP0712/FEREP0713 DreamTaq PCR Master Mix 200 x 50 µL rxns/1,000 x 50 µL rxns

Urea UV SL - POCT ServicesUREA UV SL y Sample Standard Reagent R2 60 µL 60 µL 60 µL Sample

THANK YOUaz621941.vo.msecnd.net/documents/1dc90741-7c93-40ea-9895...2x 500 µL 5 4% Formic Acid in Methanol Elute Weak Bases 2x 500 µL 5 % NH 4 OH in Methanol for Acids with pK a

Supporting Information Boosting CRISPR/Cas9 …...Bsa I (10 U/µl, NEB) 0.5 µl T7 DNA Ligase (3000 U/µl, NEB) 0.5 µl Total Volume 20 µl For GG assembly reactions to construct PTG1-PTG8,

Eppendorf Reference · 3 Reference variabel / Reference variable 0.1 – 2.5 µl 0.5 – 10 µl 2–20µl 10 – 100 µl 20 – 200 µl 100 – 1000 µl 500 – 2500µl Reference

Agilent 6470 Triple Quadrupole LC/MS System and EMR-Lipid ...EMR vs QuEChERS workflow 400 µL extract + 600 µL water in autosampler vial. (Spinach: 200 µL extract + 800 µL water)

Analysis of Submergence Tolerant Gene (Sub-1 on BC F … · 2017. 12. 26. · Marker, 1 µl R-Marker, 0.2 µl enzim . Taq Polymerase, 2 µl . polymerase, and 10.8 µl . MiliQ-water

Exosome Labeling Kits - System Biosciences · Fluorescent Label Combo Pack EXOC300A-1 Exo-Red (10X)- 500 µL Exo-Green (10X)- 500 µL ExoQuick-TC- 2mL 20 reactions (10 reactions of

10 µl 10 µl 10 µl 20 µl 100 µl 200 µl 300 µl 1.000 µl 1.250 µl … · 2017. 3. 9. · w w .nerbe-lus conformity tested (ISO 8655) Certified RNase/DNase Pyrogen, DNA, ATP

Supplementary Information - Royal Society of Chemistry · time (3 min to 1020 min), different AgNO3 content (0 µL to 450 µL) and different seed content (12 µL to 60 µL) were evaluated

TransIT X2® Dynamic Delivery System for CRISPR/Cas9 ...€¦ · Serum-free medium 50 µl 100 µl 250 µl guide RNA 50 µM stock, 12 nM final in well 0.12 µl 0.24 µl 0.6 µl as9

PROCEDURE FOR CANINE MAJOR CROSSMATCH - alvedia.com · 10 µl 10 µl 10 µl 10 µl N°3 : RBCs suspension preparation Drop gently the suspension on the top of the gel without touching

Kylt PMV-1 Pathotyping · (final 500 µl) 1 x lyophilizate (final 500 µl) ≤ -18 °C. Kylt® PMV-1 Pathotyping eal-ie T-P etection 4/10 D. Control Reactions The Positive Control

KAPA RNA HyperPrep Kit Roche Platforms · 2019. 9. 20. · KAPA HiFi HotStart ReadyMix (2X) 300 µL 300 µL 25 µL 780 µL 50 µL 1 mL ... and is the polymerase of choice for NGS

Overview - European Bioanalysis Forum...Sample prep PPT 50 µl plasma + 300 µl CH 3CN dilution 100 µl SN + 300 µl H 20 Injection volume 0.5, 1 and 2 µl (with 2 µl sample loop)

Liquid Chromatography/ Mass Spectrometry€¦ · Mass Spectrometry Author: Sharanya Reddy PerkinElmer, Inc. Shelton, CT . 2 Calibration Curve(s) To 250 µL of serum, 500 µL of ACN

ab133053 Serotonin ELISA Kit - abcam.com · 375 500 125 3 125 µL Standard #2 375 125 31.25 4 125 µL Standard #3 375 31.25 7.81 5 125 µL Standard #4 375 7.81 1.95 6 125 µL Standard

Primerdesign Ltd TM Mycoplasma · 2019-01-07 · 2 x 105 per µl 2 x 104 per µl 2 x 103 per µl 2 x 102 per µl 20 per µl 2 per µl Standard Curve Copy Number Tube 1 Positive control

BD OptEIA · µL of the 1000 pg/mL standard to both that well and the 500 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 500 pg/mL stan-dard

Combination of epT.I.P.S.® with Eppendorf Pipettes · pearl white 0.5 – 100 µL light yellow 2 – 20 µL yellow 2 – 100 µL yellow 2 – 200 µL yellow 20 – 300 µL orange

CONTENTS - Pipette 73002X Grey 1-ch 0.2–10 µl 0.05 µl 10 µl N/A N/A 73004X Yellow 1-ch 5–120 µl 0.50 µl 300 µl, 350 µl 721008 721018 73006X Orange 1-ch 10–300 µl 1.00

U N O- NCOL M O M G I Y PD1/PD-L1 BINDING ASSAY · Inhibitor Tag1-PD-L1 Tag2-PD1 Anti-Tag1- Cryptate Anti-Tag2- XL665 Diluent Detection buffer Sample 2 µL 4 µL 4 µL 5 µL 5 µL

ab207201 Total CREB Transcription Factor Assay Kit ......WI-38 (Forskolin) nuclear extract (2.5 µg/µL) 40 µL 200 µL -80°C -80°C Dithiothreitol (DTT) 100 µL 500 µL -20°C -20°C

Mentype DigitalQuant...2 x ddPCR™ Supermix for Probes (No dUTP) 10.0 µL Mentype® DigitalQuant Primermix 2.0 µL FastDigest EcoRI Enzym 0.5 µL Master mix volume 15.0 µL gDNA (10

Fine Care Biosystems - ENDO...VARIABLE 0.1 - 2.5 µl 0.5 - 10 µl 2 - 20 µl FIXED 2.5, 5, 10 µl MUL TI 0.5 - 10 µl VARIABLE 5 - 50 µl FIXED 20, 25 µl MULTI 5 - 50 µl VARIABLE