2100 expert dna 1000 de72901460 2010-02-02 14 …...general analysis settings number of available...

Instrument Name: DE72901460 Firmware:Serial#:Assay Information:

C.01.069DE72901460

Instrument Information:

Assay Origin Path: C:\Program Files\Agilent\2100 bioanalyzer\2100expert\assays\dsDNA\DNA 1000 Series II.xsy

Assay Class:Version:Assay Comments:

DNA 10002.3DNA Analysis 25 -1000 bp © Copyright 2003-2009 Agilent Technologies, Inc.

Chip Information:

Chip Comments:

Type: G2939A

Chip Lot #:Reagent Kit Lot #:

BS N shearing BS C shearing N library

C library N library 1 to 1000 dilution C library 1 to 1000 dilution

stanford 19 stanford 19 stanford 19

Sample 10 Sample 11 Sample 12

2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 4/28/2010 10:40:45 AM

2100 expert_DNA 1000_DE72901460_2010-02-02_14-04-56.xad Page of1 10

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DNA 1000C:\...-02\2100 expert_DNA 1000_DE72901460_2010-02-02_14-04-56.xad

Assay Class:

Electrophoresis File Run Summary

Sample Name Sample Comment Rest. Digest Status Observation Result Label Result ColorBS N shearingBS C shearingN libraryC libraryN library 1 to 1000 dilutionC library 1 to 1000 dilutionstanford 19stanford 19stanford 19Sample 10Sample 11Sample 12

Chip Lot # Reagent Kit Lot #

Chip Comments :



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Assay Class:

Electrophoresis File Run Summary (Chip Summary)

General Analysis SettingsNumber of Available Sample and Ladder Wells (Max.) : 13Minimum Visible Range [s] : 30Maximum Visible Range [s] : 129Start Analysis Time Range [s] : 30End Analysis Time Range [s] : 128.95Ladder Concentration [ng/µl] : 44Uses Standard Area for Ladder FragmentsLower Marker Concentration [ng/µl] : 4.2Upper Marker Concentration [ng/µl] : 2.1Used Upper Marker for QuantitationStandard Curve Fit is Point to PointShow Data Aligned to Lower and Upper Marker

Integrator SettingsIntegration Start Time [s] : 30Integration End Time [s] : 128.95Slope Threshold : 0.5Height Threshold [FU] : 20Area Threshold : 0.1Width Threshold [s] : 0.5Baseline Plateau [s] : 0.5

Filter SettingsFilter Width [s] : 0.5Polynomial Order : 4

Ladder Ladder Peak Size Area1 15 252 25 263 50 344 100 415 150 456 200 527 300 638 400 769 500 8310 700 8811 850 8612 1000 9013 1500 52



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Assay Class:

Electrophoresis Assay Details

Peak table for sample 1 : BS N shearingPeak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations1 15 4.20 424.2 Lower Marker2 1,500 2.10 2.1 Upper MarkerRegion table for sample 1 : BS N shearingFrom [bp] To [bp] Area % of Total Average Size

[bp]Size distribution in CV[%]

Conc. [ng/µl] Color

69 1,043 472.1 96 388 45.5 15.52

BS N shearing

Overall Results for sample 1 : BS N shearingNumber of peaks found: 0 Area 1: 472.1



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Assay Class:

Electropherogram Summary

Peak table for sample 2 : BS C shearingPeak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations1 15 4.20 424.2 Lower Marker2 1,500 2.10 2.1 Upper MarkerRegion table for sample 2 : BS C shearingFrom [bp] To [bp] Area % of Total Average Size



51 1,186 801.6 97 399 54.7 29.78

BS C shearing

Overall Results for sample 2 : BS C shearingNumber of peaks found: 0 Area 1: 801.6



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Assay Class:

Electropherogram Summary Continued ...

Peak table for sample 3 : N libraryPeak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations1 15 4.20 424.2 Lower Marker2 271 4.92 27.53 1,500 2.10 2.1 Upper MarkerRegion table for sample 3 : N libraryFrom [bp] To [bp] Area % of Total Average Size



203 320 159.3 85 263 9.2 5.62

N library

Overall Results for sample 3 : N libraryNumber of peaks found: 1 Area 1: 159.3



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Assay Class:


Peak table for sample 4 : C libraryPeak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations1 15 4.20 424.2 Lower Marker2 284 8.64 46.23 1,500 2.10 2.1 Upper MarkerRegion table for sample 4 : C libraryFrom [bp] To [bp] Area % of Total Average Size



225 363 291.1 83 290 9.7 9.56

C library

Overall Results for sample 4 : C libraryNumber of peaks found: 1 Area 1: 291.1



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Assay Class:




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Assay Class:

Gel Image

Standard Curve



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Assay Class:

Curves

Description Number Source Category Sub Category Time Stamp Time Zone User HostRun ended onport 3 (Numberof wellsacquired: 13)

Instrument Run 2/2/20102:46:48 PM

(GMT --08:00)Pacific StandardTime

Alex OMNIBOOK

Run started onport 3 (File:C:\ProgramFiles\Agilent\2100bioanalyzer\2100expert\Data\2010-02-02\2100expert_DNA1000_DE72901460_2010-02-02_14-04-56.xad)



Alex OMNIBOOK

Product Number: G2939A



Alex OMNIBOOK

Name : Instrument Run 2/2/20102:05:04 PM


Alex OMNIBOOK

Vendor : AgilentTechnologies



Alex OMNIBOOK

Serial# :DE72901460



Alex OMNIBOOK

Firmware :C.01.069



Alex OMNIBOOK

Cartridge :Electrode



Alex OMNIBOOK



Created:Modified:

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Assay Class:

Run Logbook

2100 expert dna 1000 de72901460 2010-02-02 14 …...general analysis settings number of available...

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