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426 Acid-Base Balance George A. Tanner, Ph.D. 25 CHAPTER 25 A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND REGULATION OF HYDROGEN IONS IN THE BODY CHEMICAL REGULATION OF PH RESPIRATORY REGULATION OF PH RENAL REGULATION OF PH REGULATION OF INTRACELLULAR PH DISTURBANCES OF ACID-BASE BALANCE CHAPTER OUTLINE 1. The body is constantly threatened by acid resulting from diet and metabolism. The stability of blood pH is main- tained by the concerted action of chemical buffers, the lungs, and the kidneys. 2. Numerous chemical buffers (e.g., HCO 3 /CO 2 , phosphates, proteins) work together to minimize pH changes in the body. The concentration ratio (base/acid) of any buffer pair, together with the pK of the acid, automatically defines the pH. 3. The bicarbonate/CO 2 buffer pair is effective in buffering in the body because its components are present in large amounts and the system is open. 4. The respiratory system influences plasma pH by regulating the PCO 2 by changing the level of alveolar ventilation. The kidneys influence plasma pH by getting rid of acid or base in the urine. 5. Renal acidification involves three processes: reabsorp- tion of filtered HCO 3 , excretion of titratable acid, and excretion of ammonia. New HCO 3 is added to the plasma and replenishes depleted HCO 3 when titratable acid (normally mainly H 2 PO 4 ) and ammonia (as NH 4 ) are excreted. 6. The stability of intracellular pH is ensured by membrane transport of H and HCO 3 , by intracellular buffers (mainly proteins and organic phosphates), and by meta- bolic reactions. 7. Respiratory acidosis is an abnormal process characterized by an accumulation of CO 2 and a fall in arterial blood pH. The kidneys compensate by increasing the excretion of H in the urine and adding new HCO 3 to the blood, thereby, diminishing the severity of the acidemia. 8. Respiratory alkalosis is an abnormal process characterized by an excessive loss of CO 2 and a rise in pH. The kidneys compensate by increasing the excretion of filtered HCO 3 , thereby, diminishing the alkalemia. 9. Metabolic acidosis is an abnormal process characterized by a gain of acid (other than H 2 CO 3 ) or a loss of HCO 3 . Respiratory compensation is hyperventilation, and renal compensation is an increased excretion of H bound to uri- nary buffers (ammonia, phosphate). 10. Metabolic alkalosis is an abnormal process characterized by a gain of strong base or HCO 3 or a loss of acid (other than H 2 CO 3 ). Respiratory compensation is hypoventilation, and renal compensation is an increased excretion of HCO 3 . 11. The plasma anion gap is equal to the plasma [Na ] [Cl ] [HCO 3 ] and is most useful in narrowing down possible causes of metabolic acidosis. KEY CONCEPTS E very day, metabolic reactions in the body produce and consume many moles of hydrogen ions (H s). Yet, the [H ] of most body fluids is very low (in the nanomolar range) and is kept within narrow limits. For example, the [H ] of arterial blood is normally 35 to 45 nmol/L (pH 7.45 to 7.35). Normally the body maintains acid-base bal- ance; inputs and outputs of acids and bases are matched so that [H ] stays relatively constant both outside and inside cells. Most of this chapter discusses the regulation of [H ] in extracellular fluid because ECF is easier to analyze than intracellular fluid and is the fluid used in the clinical eval- uation of acid-base balance. In practice, systemic arterial blood is used as the reference for this purpose. Measure-

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Page 1: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

426

Acid-Base BalanceGeorge A. Tanner, Ph.D.25

C H A P T E R

25■ A REVIEW OF ACID-BASE CHEMISTRY

■ PRODUCTION AND REGULATION OF HYDROGEN

IONS IN THE BODY

■ CHEMICAL REGULATION OF PH

■ RESPIRATORY REGULATION OF PH

■ RENAL REGULATION OF PH

■ REGULATION OF INTRACELLULAR PH

■ DISTURBANCES OF ACID-BASE BALANCE

C H A P T E R O U T L I N E

1. The body is constantly threatened by acid resulting fromdiet and metabolism. The stability of blood pH is main-tained by the concerted action of chemical buffers, thelungs, and the kidneys.

2. Numerous chemical buffers (e.g., HCO3�/CO2, phosphates,

proteins) work together to minimize pH changes in thebody. The concentration ratio (base/acid) of any bufferpair, together with the pK of the acid, automatically definesthe pH.

3. The bicarbonate/CO2 buffer pair is effective in buffering inthe body because its components are present in largeamounts and the system is open.

4. The respiratory system influences plasma pH by regulatingthe PCO2 by changing the level of alveolar ventilation. Thekidneys influence plasma pH by getting rid of acid or basein the urine.

5. Renal acidification involves three processes: reabsorp-tion of filtered HCO3

�, excretion of titratable acid, andexcretion of ammonia. New HCO3

� is added to theplasma and replenishes depleted HCO3

� when titratableacid (normally mainly H2PO4

�) and ammonia (as NH4�)

are excreted.6. The stability of intracellular pH is ensured by membrane

transport of H� and HCO3�, by intracellular buffers

(mainly proteins and organic phosphates), and by meta-bolic reactions.

7. Respiratory acidosis is an abnormal process characterizedby an accumulation of CO2 and a fall in arterial blood pH.The kidneys compensate by increasing the excretion of H�

in the urine and adding new HCO3� to the blood, thereby,

diminishing the severity of the acidemia.8. Respiratory alkalosis is an abnormal process characterized

by an excessive loss of CO2 and a rise in pH. The kidneyscompensate by increasing the excretion of filtered HCO3

�,thereby, diminishing the alkalemia.

9. Metabolic acidosis is an abnormal process characterizedby a gain of acid (other than H2CO3) or a loss of HCO3

�.Respiratory compensation is hyperventilation, and renalcompensation is an increased excretion of H� bound to uri-nary buffers (ammonia, phosphate).

10. Metabolic alkalosis is an abnormal process characterizedby a gain of strong base or HCO3

� or a loss of acid (otherthan H2CO3). Respiratory compensation is hypoventilation,and renal compensation is an increased excretion ofHCO3

�.11. The plasma anion gap is equal to the plasma [Na�] � [Cl�]

� [HCO3�] and is most useful in narrowing down possible

causes of metabolic acidosis.

K E Y C O N C E P T S

Every day, metabolic reactions in the body produce andconsume many moles of hydrogen ions (H�s). Yet, the

[H�] of most body fluids is very low (in the nanomolarrange) and is kept within narrow limits. For example, the[H�] of arterial blood is normally 35 to 45 nmol/L (pH7.45 to 7.35). Normally the body maintains acid-base bal-ance; inputs and outputs of acids and bases are matched so

that [H�] stays relatively constant both outside and insidecells.

Most of this chapter discusses the regulation of [H�]in extracellular fluid because ECF is easier to analyze thanintracellular fluid and is the fluid used in the clinical eval-uation of acid-base balance. In practice, systemic arterialblood is used as the reference for this purpose. Measure-

Page 2: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

ments on whole blood with a pH meter give values forthe [H�] of plasma and, therefore, provide an ECF pHmeasurement.

A REVIEW OF ACID-BASE CHEMISTRY

In this section, we briefly review some principles of acid-base chemistry. We define acid, base, acid dissociationconstant, weak and strong acids, pKa, pH, and the Hender-son-Hasselbalch equation and explain buffering. Studentswho already feel comfortable with these concepts can skipthis section.

Acids Dissociate to Release Hydrogen Ions

in Solution

An acid is a substance that can release or donate H�; a baseis a substance that can combine with or accept H�. Whenan acid (generically written as HA) is added to water, it dis-sociates reversibly according to the reaction, HA H� �A�. The species A� is a base because it can combine with aH� to form HA. In other words, when an acid dissociates,it yields a free H� and its conjugate (meaning “joined in apair”) base.

The Acid Dissociation Constant Ka Shows the

Strength of an Acid

At equilibrium, the rate of dissociation of an acid to formH� � A�, and the rate of association of H� and base A�

to form HA, are equal. The equilibrium constant (Ka),which is also called the ionization constant or acid dissoci-ation constant, is given by the expression

Ka � (1)

The higher the acid dissociation constant, the more anacid is ionized and the greater is its strength. Hydrochloricacid (HCl) is an example of a strong acid. It has a high Ka

and is almost completely ionized in aqueous solutions.Other strong acids include sulfuric acid (H2SO4), phos-phoric acid (H3PO4), and nitric acid (HNO3).

An acid with a low Ka is a weak acid. For example, in a0.1 mol solution of acetic acid (Ka � 1.8 � 10�5) in water,most (99%) of the acid is nonionized and little (1%) is pres-ent as acetate� and H�. The acidity (concentration of freeH�) of this solution is low. Other weak acids are lactic acid,carbonic acid (H2CO3), ammonium ion (NH4

�), and dihy-drogen phosphate (H2PO4

�).

pKa Is a Logarithmic Expression of Ka

Acid dissociation constants vary widely and often are smallnumbers. It is convenient to convert Ka to a logarithmicform, defining pKa as

pKa � log10(1/Ka) � �log10Ka (2)

In aqueous solution, each acid has a characteristic pKa,which varies slightly with temperature and the ionic

[H�] � [A�]��

[HA]

strength of the solution. Note that pKa is inversely propor-tional to acid strength. A strong acid has a high Ka and alow pKa. A weak acid has a low Ka and a high pKa.

pH Is Inversely Related to [H�]

[H�] is often expressed in pH units. The following equa-tion defines pH:

pH � log10 (1/[H�]) � �log10 [H�] (3)

where [H�] is in mol/L. Note that pH is inversely related to[H�]. Each whole number on the pH scale represents a 10-fold (logarithmic) change in acidity. A solution with a pHof 5 has 10 times the [H�] of a solution with a pH of 6.

The Henderson-Hasselbalch Equation Relates

pH to the Ratio of the Concentrations of

Conjugate Base and Acid

For a solution containing an acid and its conjugate base, wecan rearrange the equilibrium expression (equation 1) as

[H�] � (4)

If we take the negative logarithms of both sides,

–log [H�] � �log Ka � log (5)

Substituting pH for �log [H�] and pKa for �log Ka, weget

pH � pKa � log (6)

This equation is known as the Henderson-Hasselbalchequation. It shows that the pH of a solution is determinedby the pKa of the acid and the ratio of the concentration ofconjugate base to acid.

Buffers Promote the Stability of pH

The stability of pH is protected by the action of buffers. ApH buffer is defined as something that minimizes the changein pH produced when an acid or base is added. Note that abuffer does not prevent a pH change. A chemical pH buffer isa mixture of a weak acid and its conjugate base (or a weakbase and its conjugate acid). Following are examples ofbuffers:

Weak Acid Conjugate Base

�(car

Hbo

2

nCicO

a3

cid)� (7)

(8)

(9)NH3 � H�

��(ammonia)

NH4�

��(ammonium ion)

HPO42– � H�

���(monohydrogen phosphate)

H2PO4�

���(dihydrogen phosphate)

HCO3� � H�

��(bicarbonate)

[A�]�[A]

[A�]�[HA]

Ka � [HA]��[A�]

CHAPTER 25 Acid-Base Balance 427

Page 3: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

428 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

Generally, the equilibrium expression for a buffer paircan be written in terms of the Henderson-Hasselbalchequation:

pH � pKa � log (10)

For example, for H2PO40/HPO4

2–

pH � 6.8 � log (11)

The effectiveness of a buffer—how well it reduces pHchanges when an acid or base is added—depends on itsconcentration and its pKa. A good buffer is present in highconcentrations and has a pKa close to the desired pH.

Figure 25.1 shows a titration curve for the phosphatebuffer system. As a strong acid or strong base is progres-sively added to the solution (shown on the x-axis), the re-sulting pH is recorded (shown on the y-axis). Going fromright to left as strong acid is added, H� combines with the

[HPO42–]

��[HPO4�]

[conjugate base]��

[acid]

basic form of phosphate: H� � HPO42– H2PO4

�. Go-ing from left to right as strong base is added, OH� com-bines with H� released from the acid form of the phos-phate buffer: OH� � H2PO4

� HPO42– � H2O. These

reactions lessen the fall or rise in pH.At the pKa of the phosphate buffer, the ratio

[HPO42–]/[H2PO4

�] is 1 and the titration curve is flattest(the change in pH for a given amount of an added acid orbase is at a minimum). In most cases, pH buffering is effec-tive when the solution pH is within plus or minus one pHunit of the buffer pKa. Beyond that range, the pH shift thata given amount of acid or base produces may be large, sothe buffer becomes relatively ineffective.

PRODUCTION AND REGULATION OF

HYDROGEN IONS IN THE BODY

Acids are continuously produced in the body and threatenthe normal pH of the extracellular and intracellular fluids.Physiologically speaking, acids fall into two groups: (1)H2CO3 (carbonic acid), and (2) all other acids (noncar-bonic; also called “nonvolatile” or “fixed” acids). The dis-tinction between these groups occurs because H2CO3 is inequilibrium with the volatile gas CO2, which can leave thebody via the lungs. The concentration of H2CO3 in arterialblood is, therefore, set by respiratory activity. By contrast,noncarbonic acids in the body are not directly affected bybreathing. Noncarbonic acids are buffered in the body andexcreted by the kidneys.

Metabolism Is a Constant Source

of Carbon Dioxide

A normal adult produces about 300 L of CO2 daily frommetabolism. CO2 from tissues enters the capillary blood,where it reacts with water to form H2CO3, which dissoci-ates instantly to yield H� and HCO3

�: CO2 � H2OH2CO3 H� � HCO3

�. Blood pH would rapidly fall tolethal levels if the H2CO3 formed from CO2 were allowedto accumulate in the body.

Fortunately, H2CO3 produced from metabolic CO2 isonly formed transiently in the transport of CO2 by theblood and does not normally accumulate. Instead, it is con-verted to CO2 and water in the pulmonary capillaries andthe CO2 is expired. In the lungs, the reactions reverse:

H� � HCO3� H2CO3 H2O � CO2 (12)

As long as CO2 is expired as fast as it is produced, arte-rial blood CO2 tension, H2CO3 concentration, and pH donot change.

Incomplete Carbohydrate and Fat Metabolism

Produces Nonvolatile Acids

Normally, carbohydrates and fats are completely oxidizedto CO2 and water. If carbohydrates and fats are incompletelyoxidized, nonvolatile acids are produced. Incomplete oxi-dation of carbohydrates occurs when the tissues do not re-ceive enough oxygen, as during strenuous exercise or hem-

Amount of HCl added (mEq)Amount of NaOH added (mEq)

H2PO4�

HPO42�

pKa�6.8

9

8

7

6

5

pH

A titration curve for a phosphate buffer.

The pKa for H2PO4� is 6.8. A strong acid

(HCl) (right to left) or strong base (NaOH) (left to right) wasadded and the resulting solution pH recorded (y-axis). Noticethat buffering is best (i.e., the change in pH upon the addition ofa given amount of acid or base is least) when the solution pH isequal to the pKa of the buffer.

FIGURE 25.1

Page 4: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

orrhagic or cardiogenic shock. In such states, glucose me-tabolism yields lactic acid (pKa � 3.9), which dissociatesinto lactate and H�, lowering the blood pH. Incompletefatty acid oxidation occurs in uncontrolled diabetes melli-tus, starvation, and alcoholism and produces ketone bodyacids (acetoacetic and �-hydroxybutyric acids). Theseacids have pKa values around 4 to 5. At blood pH, theymostly dissociate into their anions and H�, making theblood more acidic.

Protein Metabolism Generates Strong Acids

The metabolism of dietary proteins is a major source ofH�. The oxidation of proteins and amino acids producesstrong acids such as H2SO4, HCl, and H3PO4. The oxi-dation of sulfur-containing amino acids (methionine, cys-teine, cystine) produces H2SO4, and the oxidation ofcationic amino acids (arginine, lysine, and some histidineresidues) produces HCl. H3PO4 is produced by the oxi-dation of phosphorus-containing proteins and phospho-esters in nucleic acids.

On a Mixed Diet, Net Acid Gain Threatens pH

A diet containing both meat and vegetables results in a netproduction of acids, largely from protein oxidation. Tosome extent, acid-consuming metabolic reactions balanceH� production. Food also contains basic anions, such ascitrate, lactate, and acetate. When these are oxidized toCO2 and water, H� ions are consumed (or, amounting tothe same thing, HCO3

� is produced). The balance of acid-forming and acid-consuming metabolic reactions results ina net production of about 1 mEq H�/kg body weight/dayin an adult person who eats a mixed diet. Persons who arevegetarians generally have less of a dietary acid burden anda more alkaline urine pH than nonvegetarians because mostfruits and vegetables contain large amounts of organic an-ions that are metabolized to HCO3

�. The body generallyhas to dispose of more or less nonvolatile acid, a functionperformed by the kidneys.

Whether a particular food has an acidifying or an alka-linizing effect depends on if and how its constituents aremetabolized. Cranberry juice has an acidifying effect be-cause of its content of benzoic acid, an acid that cannot bebroken down in the body. Orange juice has an alkalinizingeffect, despite its acidic pH of about 3.7, because it containscitrate, which is metabolized to HCO3

�. The citric acid inorange juice is converted to CO2 and water and has only atransient effect on blood pH and no effect on urine pH.

Many Buffering Mechanisms Protect

and Stabilize Blood pH

Despite constant threats to acid-base homeostasis, a healthyperson maintains a normal blood pH. Figure 25.2 showssome of the ways in which blood pH is kept at normal lev-els despite the daily net acid gain. The key buffering agentsare chemical buffers, along with the lungs and kidneys.

1) Chemical buffering. Chemical buffers in extra-cellular and intracellular fluids and in bone are the firstline of defense of blood pH. Chemical buffering mini-

mizes a change in pH but does not remove acid or basefrom the body.

2) Respiratory response. The respiratory system isthe second line of defense of blood pH. Normally, breath-ing removes CO2 as fast as it forms. Large loads of acidstimulate breathing (respiratory compensation), which re-moves CO2 from the body and lowers the [H2CO3] in ar-terial blood, reducing the acidic shift in blood pH.

3) Renal response. The kidneys are the third line ofdefense of blood pH. Although chemical buffers in thebody can bind H� and the lungs can change [H2CO3] ofblood, the burden of removing excess H� falls directly onthe kidneys. Hydrogen ions are excreted in combinationwith urinary buffers. At the same time, the kidneys add newHCO3

� to the ECF to replace HCO3� used to buffer

strong acids. The kidneys also excrete the anions (phos-phate, chloride, sulfate) that are liberated from strongacids. The kidneys affect blood pH more slowly than otherbuffering mechanisms in the body; full renal compensationmay take 1 to 3 days.

CHAPTER 25 Acid-Base Balance 429

Food intake

Digestion

Absorption

Cell metabolismof food

H+

H+

H+

SulfatePhosphateChloride

SulfatePhosphateChloride

SulfatePhosphateChloride

CO2

CO2

HCO3-

[HCO3-]

[HCO3-]

CO2

Renalresponse

Respiratoryresponse

Chemicalbuffering

Bound bybody buffer

bases

Extracellularfluid

New

Extracellularfluid

Excreted

Excreted(combined with

urinarybuffer bases)

The maintenance of normal blood pH by

chemical buffers, the respiratory system,

and the kidneys. On a mixed diet, pH is threatened by the pro-duction of strong acids (sulfuric, hydrochloric, and phosphoric)mainly as a result of protein metabolism. These strong acids arebuffered in the body by chemical buffer bases, such as ECFHCO3

�. The kidneys eliminate hydrogen ions (combined withurinary buffers) and anions in the urine. At the same time, theyadd new HCO3

� to the ECF, to replace the HCO3� consumed

in buffering strong acids. The respiratory system disposes of CO2.

FIGURE 25.2

Page 5: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

430 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

CHEMICAL REGULATION OF PH

The body contains many conjugate acid-base pairs that actas chemical buffers (Table 25.1). In the ECF, the mainchemical buffer pair is HCO3

�/CO2. Plasma proteins andinorganic phosphate are also ECF buffers. Cells have largebuffer stores, particularly proteins and organic phosphatecompounds. HCO3

� is present in cells, although at a lowerconcentration than in ECF. Bone contains large bufferstores, specifically phosphate and carbonate salts.

Chemical Buffers Are the First to Defend pH

When an acid or base is added to the body, the buffers justmentioned bind or release H�, minimizing the change inpH. Buffering in ECF occurs rapidly, in minutes. Acids orbases also enter cells and bone, but this generally occursmore slowly, over hours, allowing cell buffers and bone toshare in buffering.

A pKa of 6.8 Makes Phosphate a Good Buffer

The pKa for phosphate, H2PO4� H� � HPO4

2–, is 6.8,close to the desired blood pH of 7.4, so phosphate is a goodbuffer. In the ECF, phosphate is present as inorganic phos-phate. Its concentration, however, is low (about 1 mmol/L),so it plays a minor role in extracellular buffering.

Phosphate is an important intracellular buffer, how-ever, for two reasons. First, cells contain large amounts ofphosphate in such organic compounds as adenosinetriphosphate (ATP), adenosine diphosphate (ADP), andcreatine phosphate. Although these compounds primarilyfunction in energy metabolism, they also act as pHbuffers. Second, intracellular pH is generally lower thanthe pH of ECF and is closer to the pKa of phosphate. (Thecytosol of skeletal muscle, for example, has a pH of 6.9.)Phosphate is, thus, more effective in this environmentthan in one with a pH of 7.4. Bone has large phosphatesalt stores, which also help in buffering.

Proteins Are Excellent Buffers

Proteins are the largest buffer pool in the body and are ex-cellent buffers. Proteins can function as both acids andbases, so they are amphoteric. They contain many ioniz-able groups, which can release or bind H�. Serum albuminand plasma globulins are the major extracellular proteinbuffers, present mainly in the blood plasma. Cells also havelarge protein stores. Recall that the buffering properties ofhemoglobin play an important role in the transport of CO2

and O2 by the blood (see Chapter 21).

The Bicarbonate/Carbon Dioxide Buffer Pair

Is Crucial in pH Regulation

For several reasons, the HCO3�/CO2 buffer pair is espe-

cially important in acid-base physiology:1) Its components are abundant; the concentration of

HCO3� in plasma or ECF normally averages 24 mmol/L.

Although the concentration of dissolved CO2 is lower (1.2mmol/L), metabolism provides a nearly limitless supply.

2) Despite a pK of 6.10, a little far from the desiredplasma pH of 7.40, it is effective because the system is“open.”

3) It is controlled by the lungs and kidneys.

Forms of Carbon Dioxide. CO2 exists in the body in sev-eral different forms: as gaseous CO2 in the lung alveoli, andas dissolved CO2, H2CO3, HCO3

�, carbonate (CO32–),

and carbamino compounds in the body fluids.CO3

2– is present at appreciable concentrations only inrather alkaline solutions, and so we will ignore it. We willalso ignore any CO2 that is bound to proteins in the car-bamino form. The most important forms are gaseous CO2,dissolved CO2, H2CO3, and HCO3

�.

The CO2/H2CO3/HCO3� Equilibria. Dissolved CO2 in

pulmonary capillary blood equilibrates with gaseous CO2in the lung alveoli. Consequently, the partial pressures ofCO2 (PCO2) in alveolar air and systemic arterial blood arenormally identical. The concentration of dissolved CO2([CO2(d)]) is related to the PCO2 by Henry’s law (see Chap-ter 21). The solubility coefficient for CO2 in plasma at37�C is 0.03 mmol CO2/L per mm Hg PCO2. Therefore,[CO2(d)] � 0.03 � PCO2. If PCO2 is 40 mm Hg, then[CO2(d)] is 1.2 mmol/L.

In aqueous solutions, CO2(d) reacts with water to formH2CO3: CO2(d) � H2O H2CO3. The reaction to theright is called the hydration reaction, and the reaction tothe left is called the dehydration reaction. These reactionsare slow if uncatalyzed. In many cells and tissues, such asthe kidneys, pancreas, stomach, and red blood cells, the re-actions are catalyzed by carbonic anhydrase, a zinc-con-taining enzyme. At equilibrium, CO2(d) is greatly favored;at body temperature, the ratio of [CO2(d)] to [H2CO3] isabout 400:1. If [CO2(d)] is 1.2 mmol/L, then [H2CO3]equals 3 �mol/L. H2CO3 dissociates instantaneously intoH� and HCO3

�: H2CO3 H� � HCO3�. The Hender-

son-Hasselbalch expression for this reaction is

pH � 3.5 � log (13)[HCO3

�]��[H2CO3]

TABLE 25.1 Major Chemical pH Buffers in the Body

Buffer Reaction

Extracellular fluidBicarbonate/CO2 CO2 � H2O ←→ H2CO3

←→ H�

� HCO3�

Inorganic phosphate H2PO4� ←→ H� � HPO4

2�

Plasma proteins (Pr) HPr ←→ H� � Pr�

Intracellular fluidCell proteins (e.g., HHb ←→ H� � Hb�

hemoglobin, Hb)Organic phosphates Organic-HPO4

� ←→ H� �

organic-PO42�

Bicarbonate/CO2 CO2 � H2O ←→ H2CO3←→ H�

� HCO3�

BoneMineral phosphates H2PO4

� ←→ H� � HPO42�

Mineral carbonates HCO3� ←→ H� � CO3

2�

Page 6: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

Note that H2CO3 is a fairly strong acid (pKa � 3.5). Itslow concentration in body fluids lessens its impact on acidity.

The Henderson-Hasselbalch Equation for HCO3�/CO2..

Because [H2CO3] is so low and hard to measure and be-cause [H2CO3] � [CO2(d)]/400, we can use [CO2(d)] torepresent the acid in the Henderson-Hasselbalch equation:

pH � 3.5 � log/400

� 3.5 � log 400 � log (14)

� 6.1 � log

We can also use 0.03 � PCO2 in place of [CO2(d)]:

pH � 6.1 � log (15)

This form of the Henderson-Hasselbalch equation isuseful in understanding acid-base problems. Note that the“acid” in this equation appears to be CO2(d), but is reallyH2CO3 “represented” by CO2. Therefore, this equation isvalid only if CO2(d) and H2CO3 are in equilibrium witheach other, which is usually (but not always) the case.

Many clinicians prefer to work with [H�] rather thanpH. The following expression results if we take antiloga-rithms of the Henderson-Hasselbalch equation:

[H�] � 24 PCO2/[HCO3�] (16)

In this expression, [H�] is expressed in nmol/L,[HCO3

�] in mmol/L or mEq/L, and PCO2 in mm Hg. If PCO

is 40 mm Hg and plasma [HCO3�] is 24 mmol/L, [H�] is

40 nmol/L.

An “Open” Buffer System. As previously noted, the pKof the HCO3

�/CO2 system (6.10) is far from 7.40, the nor-mal pH of arterial blood. From this, one might view this asa rather poor buffer pair. On the contrary, it is remarkablyeffective because it operates in an open system; that is, thetwo buffer components can be added to or removed fromthe body at controlled rates.

The HCO3�/CO2 system is open in several ways:

1) Metabolism provides an endless source of CO2,which can replace any H2CO3 consumed by a base addedto the body.

2) The respiratory system can change the amount ofCO2 in body fluids by hyperventilation or hypoventilation.

3) The kidneys can change the amount of HCO3� in

the ECF by forming new HCO3� when excess acid has

been added to the body or excreting HCO3� when excess

base has been added.How the kidneys and respiratory system influence blood

pH by operating on the HCO3�/CO2 system is described

below. For now, the advantages of an open buffer systemare best explained by an example (Fig. 25.3). Suppose wehave 1 L of blood containing 24 mmol of HCO3

� and 1.2

[HCO3�]��0.03 PCO2

[HCO3�]

��[CO2(d)]

[HCO3�]

��[CO2(d)]

[HCO3�]

��[CO2(d)]

CHAPTER 25 Acid-Base Balance 431

mmol of dissolved CO2(d) (PCO2 � 40 mm Hg). Using thespecial form of the Henderson-Hasselbalch equation de-scribed above, we find that the pH of the blood is 7.40:

pH � 6.10 � log

� 6.10 � log � 7.40

(17)

Suppose we now add 10 mmol of HCl, a strong acid.HCO3

� is the major buffer base in the blood plasma (wewill neglect the contributions of other buffers). From thereaction H� � HCO3

� H2CO3 H2O � CO2, wepredict that the [HCO3

�] will fall by 10 mmol, and that 10mmol of CO2(d) will form. If the system were closed and noCO2 could escape, the new pH would be

pH � 6.10 � log � 6.20 (18)

This is an intolerably low—indeed a fatal—pH.Fortunately, however, the system is open and CO2 can

escape via the lungs. If all of the extra CO2 is expired andthe [CO2(d)] is kept at 1.2 mmol/L, the pH would be

pH � 6.10 � log � 7.17 (19)

Although this pH is low, it is compatible with life.Still another mechanism promotes the escape of CO2. In

the body, an acidic blood pH stimulates breathing, whichcan make the PCO2 lower than 40 mm Hg. If PCO2 falls to30 mm Hg ([CO2(d)] � 0.9 mmol/L) the pH would be

pH � 6.10 � log � 7.29 (20)

The system is also open at the kidneys and new HCO3�

can be added to the plasma to correct the plasma[HCO3

�]. Once the pH of the blood is normal, the stimu-lus for hyperventilation disappears.

Changes in Acid Production May

Help Protect Blood pH

Another way in which blood pH may be protected is bychanges in endogenous acid production (Fig. 25.4). An in-crease in blood pH caused by the addition of base to thebody results in increased production of lactic acid and ke-tone body acids, which then reduces the alkaline shift inpH. A decrease in blood pH results in decreased produc-tion of lactic acid and ketone body acids, which opposesthe acidic shift in pH.

This scenario is especially important when the endoge-nous production of these acids is high, as occurs during stren-uous exercise or other conditions of circulatory inadequacy(lactic acidosis) or during ketosis as a result of uncontrolleddiabetes, starvation, or alcoholism. These effects of pH onendogenous acid production result from changes in enzyme

[24 � 10]��

[0.9]

[24 � 10]��

[1.2]

[24 � 10]��[1.2 � 10]

[24]�[1.2]

[HCO3�]

��0.03 PCO2

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432 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

activities brought about by the pH changes, and they arepart of a negative-feedback mechanism regulating blood pH.

All Buffers Are in Equilibrium With the Same [H�]

We have discussed the various buffers separately but, in thebody, they all work together. In a solution containing mul-tiple buffers, all are in equilibrium with the same [H�]. This

Closedsystem

response

Opensystem

response

pH = 7.40Normal

condition

pH = 6.20 pH = 7.17 pH = 7.29 pH = 7.40Normal

condition

Add10 mmol/Lstrong acid

RemoveextraCO2

LoseCO2

because ofhyperventilation

Kidneysadd newHCO3

-

to blood(excrete H+)

[HCO3-]

24

[HCO3-]

14[HCO3

-]14

[HCO3-]

14

[HCO3-]

24

[CO2(d)] 1.2

[CO2(d)] 11.2

[CO2(d)] 1.2 [CO2(d)] 0.9 [CO2(d)] 1.2

The HCO32/CO2 system. This system is re-

markably effective in buffering added strongacid in the body because it is open. [HCO3

�] and [CO2(d)] are

FIGURE 25.3 in mmol/L. See text for details. (Adapted from Pitts RF. Physiol-ogy of the Kidney and Body Fluids. 3rd Ed. Chicago: YearBook, 1974.)

Acid load Base load

SystemicpH

Endogenous acidproduction

(ketoacidosis,lactic acidosis)

SystemicpH

SystemicpH

Negative-feedback control of endogenous

acid production. The addition of an exoge-nous acid load or increased endogenous acid production result ina fall in pH, which, in turn, inhibits the production of ketonebody acids and lactic acid. A base load, by raising pH, stimulatesthe endogenous production of acids. This negative-feedbackmechanism attenuates changes in blood pH. (From Hood VL,Tannen RL. Protection of acid-base balance by pH regulation ofacid production. N Engl J Med 1998;339:819–826.)

FIGURE 25.4

idea is known as the isohydric principle (isohydric meaning“same H�”). For plasma, for example, we can write

pH � 6.80 � log

� 6.10 � log

� pKprotein � log (21)

If an acid or a base is added to such a complex mixture ofbuffers, all buffers take part in buffering and shift from oneform (base or acid) to the other. The relative importance ofeach buffer depends on its amount, pK, and availability.

The isohydric principle underscores the fact that it is theconcentration ratio for any buffer pair, together with its pK,that sets the pH. We can focus on the concentration ratio forone buffer pair and all other buffers will automatically adjusttheir ratios according to the pH and their pK values.

The rest of this chapter emphasizes the role of theHCO3

�/CO2 buffer pair in setting the blood pH. Otherbuffers, however, are present and active. The HCO3

�/CO2

system is emphasized because physiological mechanisms(lungs and kidneys) regulate pH by acting on componentsof this buffer system.

[proteinate�]��[H-protein]

[HCO3�]

��0.03 PCO2

[HPO42–]

��[H2PO4�]

Page 8: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

RESPIRATORY REGULATION OF PH

Reflex changes in ventilation help to defend blood pH. Bychanging the PCO2 and, hence, [H2CO3] of the blood, therespiratory system can rapidly and profoundly affect bloodpH. As discussed in Chapter 22, a fall in blood pH stimu-lates ventilation, primarily by acting on peripheralchemoreceptors. An elevated arterial blood PCO2 is a pow-erful stimulus to increase ventilation; it acts on both periph-eral and central chemoreceptors, but primarily on the latter.CO2 diffuses into brain interstitial and cerebrospinal fluids,where it causes a fall in pH that stimulates chemoreceptorsin the medulla oblongata. When ventilation is stimulated,the lungs blow off more CO2, making the blood less acidic.Conversely, a rise in blood pH inhibits ventilation; the con-sequent rise in blood [H2CO3] reduces the alkaline shift inblood pH. Respiratory responses to disturbed blood pH be-gin within minutes and are maximal in about 12 to 24 hours.

RENAL REGULATION OF PH

The kidneys play a critical role in maintaining acid-basebalance. If there is excess acid in the body, they removeH�, or if there is excess base, they remove HCO3

�. Theusual challenge is to remove excess acid. As we havelearned, strong acids produced by metabolism are firstbuffered by body buffer bases, particularly HCO3

�. Thekidneys then must eliminate H� in the urine and restore thedepleted HCO3

�.Little of the H� excreted in the urine is present as free

H�. For example, if the urine has its lowest pH value (pH � 4.5), [H�] is only 0.03 mEq/L. With a typical dailyurine output of 1 to 2 L, the amount of acid the body mustdispose of daily (about 70 mEq) obviously is not excretedin the free form. Most of the H� combines with urinarybuffers to be excreted as titratable acid and as NH4

�.Titratable acid is measured from the amount of strong

base (NaOH) needed to bring the urine pH back to thepH of the blood (usually, 7.40). It represents the amountof H� ions that are excreted, combined with urinarybuffers such as phosphate, creatinine, and other bases.The largest component of titratable acid is normallyphosphate, that is, H2PO4

�.Hydrogen ions secreted by the renal tubules also com-

bine with the free base NH3 and are excreted as NH4�.

Ammonia (a term that collectively includes both NH3 andNH4

�) is produced by the kidney tubule cells and is se-creted into the urine. Because the pKa for NH4

� is high(9.0), most of the ammonia in the urine is present as NH4

�.For this reason, too, NH4

� is not appreciably titrated whentitratable acid is measured. Urinary ammonia is measuredby a separate, often chemical, method.

Renal Net Acid Excretion Equals the

Sum of Urinary Titratable Acid and

Ammonia Minus Urinary Bicarbonate

In stable acid-base balance, net acid excretion by the kid-neys equals the net rate of H� addition to the body by me-tabolism or other processes, assuming that other routes of

loss of acid or base (e.g., gastrointestinal losses) are smalland can be neglected, which normally is the case. The netloss of H� in the urine can be calculated from the followingequation, which shows typical values in the parentheses:

Renal net acid excretion (70 mEq/day) �urinary titratable acid (24 mEq/day) �urinary ammonia (48 mEq/day) �urinary HCO3

� (2 mEq/day) (22)

Urinary ammonia (as NH4�) ordinarily accounts for

about two thirds of the excreted H�, and titratable acid forabout one third. Excretion of HCO3

� in the urine repre-sents a loss of base from the body. Therefore, it must besubtracted in the calculation of net acid excretion. If theurine contains significant amounts of organic anions, suchas citrate, that potentially could have yielded HCO3

� inthe body, these should also be subtracted. Since theamount of free H� excreted is negligible, this is omittedfrom the equation.

Hydrogen Ions Are Added to Urine as

It Flows Along the Nephron

As the urine flows along the tubule, from Bowman’s capsuleon through the collecting ducts, three processes occur: fil-tered HCO3

� is reabsorbed, titratable acid is formed, andammonia is added to the tubular urine. All three processesinvolve H� secretion (urinary acidification) by the tubularepithelium. The nature and magnitude of these processesvary in different nephron segments. Figure 25.5 summarizesmeasurements of tubular fluid pH along the nephron andshows ammonia movements in various nephron segments.

Acidification in the Proximal Convoluted Tubule. ThepH of the glomerular ultrafiltrate, at the beginning of theproximal tubule, is identical to that of the plasma fromwhich it is derived (7.4). H� ions are secreted by the prox-imal tubule epithelium into the tubule lumen; about twothirds of this is accomplished by a Na�/H� exchanger andabout one third by H�-ATPase in the brush border mem-brane. Tubular fluid pH falls to a value of about 6.7 by theend of the proximal convoluted tubule (see Fig. 25.5).

The drop in pH is modest for two reasons: buffering ofsecreted H� and the high permeability of the proximaltubule epithelium to H�. The glomerular filtrate and tubulefluid contain abundant buffer bases, especially HCO3

�,which soak up secreted H�, minimizing a fall in pH. Theproximal tubule epithelium is rather leaky to H�, so thatany gradient from urine to blood, established by H� secre-tion, is soon limited by the diffusion of H� out of thetubule lumen into the blood surrounding the tubules

Most of the H� ions secreted by the nephron are se-creted in the proximal convoluted tubule and are used tobring about the reabsorption of filtered HCO3

�. SecretedH� ions are also buffered by filtered phosphate to formtitratable acid. Ammonia is produced by proximal tubulecells, mainly from glutamine. It is secreted into the tubularurine by the diffusion of NH3, which then combines with asecreted H� to form NH4

�, or via the brush border mem-brane Na�/H� exchanger, which can operate in aNa�/NH4

� exchange mode.

CHAPTER 25 Acid-Base Balance 433

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434 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

Acidification in the Henle Loop. Along the descendinglimb of the Henle loop, the pH of tubular fluid rises (from6.7 to 7.4). This rise is explained by an increase in intralu-minal [HCO3

�] caused by water reabsorption. Ammonia issecreted along the descending limb.

The tubular fluid is acidified by secretion of H� alongthe ascending limb via a Na�/H� exchanger. Along thethin ascending limb, ammonia is passively reabsorbed.Along the thick ascending limb, NH4

� is mostly activelyreabsorbed by the Na-K-2Cl cotransporter in the luminalplasma membrane (NH4

� substitutes for K�). Some NH4�

can be reabsorbed via a luminal plasma membrane K�

channel. Also, some NH4� can be passively reabsorbed be-

tween cells in this segment; the driving force is the lumenpositive transepithelial electrical potential difference. Am-monia may undergo countercurrent multiplication in theHenle loop, leading to an ammonia concentration gradientin the kidney medulla. The highest concentrations are atthe tip of the papilla.

Acidification in the Distal Nephron. The distal nephron(distal convoluted tubule, connecting tubule, and collect-ing duct) differs from the proximal portion of the nephronin its H� transport properties. It secretes far fewer H� ions,and they are secreted primarily via an electrogenic H�-ATPase or an electroneutral H�/K�-ATPase. The distalnephron is also lined by “tight” epithelia, so little secretedH� diffuses out of the tubule lumen, making steep urine-to-blood pH gradients possible (see Fig. 25.5). Final urine pHis typically about 6, but may be as low as 4.5.

The distal nephron usually almost completely reabsorbsthe small quantities of HCO3

� that were not reabsorbed bymore proximal nephron segments. Considerable titratableacid forms as the urine is acidified. Ammonia, which was re-absorbed by the ascending limb of the Henle loop and hasaccumulated in the medullary interstitial space, diffuses aslipid-soluble NH3 into collecting duct urine and combineswith secreted H� to form NH4

�. The collecting duct ep-ithelium is impermeable to the lipid-insoluble NH4

�, so am-monia is trapped in an acidic urine and excreted as NH4

(see Fig. 25.5). The intercalated cells of the collecting ductare involved in acid-base transport and are of two majortypes: an acid-secreting �-intercalated cell and a bicarbon-ate-secreting �-intercalated cell. The �-intercalated cell hasa vacuolar type of H�-ATPase (the same kind as is found inlysosomes, endosomes, and secretory vesicles) and anH�/K�-ATPase (similar to that found in stomach and colonepithelial cells) in the luminal plasma membrane and aCl�/HCO3

� exchanger in the basolateral plasma membrane(Fig. 25.6). The �-intercalated cell has the opposite polarity.

A more acidic blood pH results in the insertion of cyto-plasmic H� pumps into the luminal plasma membrane of �-intercalated cells and enhanced H� secretion. If the bloodis made alkaline, HCO3

� secretion by �-intercalated cellsis increased. Because the amounts of HCO3

� secreted areordinarily small compared to the amounts filtered and re-absorbed, HCO3

� secretion will not be included in the re-maining discussion.

The Reabsorption of Filtered HCO3� Restores Lost

HCO3� to the Blood

HCO3� is freely filtered at the glomerulus, about 4,320

mEq/day (180 L/day � 24 mEq/L). Urinary loss of even asmall portion of this HCO3

� would lead to acidic bloodand impair the body’s ability to buffer its daily load of meta-bolically produced H�. The kidney tubules have the im-portant task of recovering the filtered HCO3

� and return-ing it to the blood.

Figure 25.7 shows how HCO3� filtration, reabsorption,

and excretion normally vary with plasma [HCO3�]. This

type of graph should be familiar (Fig. 23.8). The y-axis ofthe graph is unusual, however, because amounts of HCO3

per minute are factored by the GFR. The data are expressedin this way because the maximal rate of tubular reabsorp-tion of HCO3

� varies with GFR. The amount of HCO3�

excreted in the urine per unit time is calculated as the dif-ference between filtered and reabsorbed amounts. At lowplasma concentrations of HCO3

� (below about 26 mEq/L),all of the filtered HCO3

� is reabsorbed. Because the plasma[HCO3

�] and pH were decreased by ingestion of an acid-

pH = 7.4

Distalconvoluted

tubule

Proximalconvoluted

tubule

pH = 7.4

CollectingductpH = 6.7

pH ~ 6

2Cl-

Na+

NH4+

NH4+

NH4+

NH3

+ NH3

NH3

H+ H+

H+

+

NH4+

+ NH3H+

Na+

NH4+

Na+

NH4+

Glutamine

NH4+

H+ NH3

NH4+H++ NH3

Na+

Acidification along the nephron. The pH oftubular urine decreases along the proximal con-

voluted tubule, rises along the descending limb of the Henleloop, falls along the ascending limb, and reaches its lowest valuesin the collecting ducts. Ammonia (�NH3 � NH4

�) is chieflyproduced in proximal tubule cells and is secreted into the tubularurine. NH4

� is reabsorbed in the thick ascending limb and accu-mulates in the kidney medulla. NH3 diffuses into acidic collectingduct urine, where it is trapped as NH4

�.

FIGURE 25.5

Page 10: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

ifying salt (NH4Cl), it makes good sense that the kidneysconserve filtered HCO3

� in this situation.If the plasma [HCO3

�] is raised to high levels because ofintravenous infusion of solutions containing NaHCO3 forexample, filtered HCO3

� exceeds the reabsorptive capacityof the tubules and some HCO3

� will be excreted in the urine(see Fig. 25.7). This also makes good sense. If the blood is tooalkaline, the kidneys excrete HCO3

�. This loss of basewould return the pH of the blood to its normal value.

At the cellular level (see Fig. 25.8), filtered HCO3� is

not reabsorbed directly across the tubule’s luminal plasmamembrane as, for example, is glucose. Instead, filteredHCO3

� is reabsorbed indirectly via H� secretion in thefollowing way. About 90% of the filtered HCO3

� is reab-sorbed in the proximal convoluted tubule, and we will em-phasize events at this site. H� is secreted into the tubule lu-men mainly via the Na�/H� exchanger in the luminalmembrane. It combines with filtered HCO3

� to formH2CO3. Carbonic anhydrase (CA) in the luminal mem-brane (brush border) of the proximal tubule catalyzes thedehydration of H2CO3 to CO2 and water in the lumen.The CO2 diffuses back into the cell.

Inside the cell, the hydration of CO2 (catalyzed by in-tracellular CA) yields H2CO3, which instantaneously formsH� and HCO3

�. The H� is secreted into the lumen, andthe HCO3

� ion moves into the blood surrounding thetubules. In proximal tubule cells, this movement is favoredby the inside negative membrane potential of the cell and by

an electrogenic cotransporter in the basolateral membranethat simultaneously transports three HCO3

� and one Na�.The reabsorption of filtered HCO3

� does not result inH� excretion or the formation of any “new” HCO3

�. Thesecreted H� is not excreted because it combines with fil-tered HCO3

� that is, indirectly, reabsorbed. There is nonet addition of HCO3

� to the body in this operation. It issimply a recovery or reclamation process.

Excretion of Titratable Acid and Ammonia

Generates New Bicarbonate

When H� is excreted as titratable acid and ammonia, newHCO3

� is formed and added to the blood. New HCO3�

replaces the HCO3� used to buffer the strong acids pro-

duced by metabolism.The formation of new HCO3

� and the excretion of H�

are like two sides of the same coin. This fact is apparent ifwe assume that H2CO3 is the source of H�:

H� (urine)

CO2 � H2O H2CO3zx

(23)

HCO3� (blood)

A loss of H� in the urine is equivalent to adding new HCO3� to the

blood. The same is true if H� is lost from the body via an-other route, such as by vomiting of acidic gastric juice. Thisprocess leads to a rise in plasma [HCO3

�]. Conversely, a lossof HCO3

� from the body is equivalent to adding H� to the blood.

CHAPTER 25 Acid-Base Balance 435

Blood α-Intercalated cell Collectingduct urine

H+

H+

Cl-

Cl-

HCO3-

ATP

ADP + Pi

ATP

ADP + PiK+

Blood β-Intercalated cell Collectingduct urine

H+

H+

Cl-

Cl-

HCO3-

ATP

ADP + Pi

ATP

ADP + PiK+

Collecting duct intercalated cells. The �-intercalated cell secretes H� via an electro-

genic, vacuolar H�-ATPase and electroneutral H�/K�-ATPaseand adds HCO3

� to the blood via a basolateral plasma mem-brane Cl�/HCO3

� exchanger. The �-intercalated cell, which islocated in cortical collecting ducts, has the opposite polarityand secretes HCO3

�.

FIGURE 25.6

The filtration, reabsorption, and excretion

of HCO3�. Decreases in plasma [HCO3

�]were produced by ingestion of NH4Cl and increases were pro-duced by intravenous infusion of a solution of NaHCO3. All thefiltered HCO3

� was reabsorbed below a plasma concentration ofabout 26 mEq/L. Above this value (“threshold”), appreciablequantities of filtered HCO3

� were excreted in the urine.(Adapted from Pitts RF, Ayer JL, Schiess WA. The renal regula-tion of acid-base balance in man. III. The reabsorption and excre-tion of bicarbonate. J Clin Invest 1949;28:35–44.)

FIGURE 25.7

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436 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

Titratable Acid Excretion. Figure 25.9 shows a cell modelfor the formation of titratable acid. In this figure, H2PO4

is the titratable acid formed. H� and HCO3� are produced

in the cell from H2CO3. The secreted H� combines withthe basic form of the phosphate (HPO4

2–) to form the acidphosphate (H2PO4

�). The secreted H� replaces one of theNa� ions accompanying the basic phosphate. The newHCO3

� generated in the cell moves into the blood, to-gether with Na�. For each mEq of H� excreted in the urineas titratable acid, a mEq of new HCO3

� is added to theblood. This process eliminates H� in the urine, replacesECF HCO3

�, and restores a normal blood pH.The amount of titratable acid excreted depends on two

factors: the pH of the urine and the availability of buffer. If

the urine pH is lowered, more titratable acid can form. Thesupply of phosphate and other buffers is usually limited. Toexcrete large amounts of acid, the kidneys must rely on in-creased ammonia excretion.

Ammonia Excretion. Figure 25.10 shows a cell model forthe excretion of ammonia. Most ammonia is synthesized inproximal tubule cells by deamidation and deamination ofthe amino acid glutamine:

NH4� NH4

z zGlutamine → Glutamate� → �-Ketoglutarate2 (24)

Glutaminase Glutamate dehydrogenase

As discussed earlier, ammonia is secreted into the urineby two mechanisms. As NH3, it diffuses into the tubularurine; as NH4

�, it substitutes for H� on the Na�/H� ex-changer. In the lumen, NH3 combines with secreted H� toform NH4

�, which is excreted.For each mEq of H� excreted as NH4

�, one mEq of newHCO3

� is added to the blood. The hydration of CO2 in thetubule cell produces H� and HCO3

�, as described earlier.Two H�s are consumed when the anion �-ketoglutarate2– isconverted into CO2 and water or into glucose in the cell.The new HCO3

� returns to the blood along with Na�.If excess acid is added to the body, urinary ammonia ex-

cretion is increased for two reasons. First, a more acidicurine traps more ammonia (as NH4

�) in the urine. Second,renal ammonia synthesis from glutamine increases over sev-

Peritubularblood

Tubularepithelium

Tubularurine

(reclaimed)

CA(filtered)

CA

Na+Na+

H+H+

CO2

CO2 CO2

H2O

H2O H2CO3H2CO3

HCO3-

HCO3-HCO3

-

+

A cell model for HCO32 reabsorption. Fil-

tered HCO3� combines with secreted H� and

is reabsorbed indirectly. Carbonic anhydrase (CA) is present inthe cells and in the proximal tubule on the brush border.

FIGURE 25.8

Peritubularblood

Tubularepithelium

Tubularurine

(new)

CA

(filtered)

(excreted)

Na+Na+

H+H+

CO2 CO2 H2O H2CO3H2PO4

-Na+

HPO42-2Na+HCO3

-HCO3-

+

A cell model for the formation of titratable

acid. Titratable acid (e.g., H2PO4�) is formed

when secreted H� is bound to a buffer base (e.g., HPO42–) in the

tubular urine. For each mEq of titratable acid excreted, a mEq ofnew HCO3

� is added to the peritubular capillary blood.

FIGURE 25.9

Peritubularblood

Tubularepithelium

Tubularurine

Cl-

Cl-

CACO2CO2 H2O+

HCO3-HCO3

- 2H+

(new)

NH4+

NH4+

Na+

2

2 22

2

2

+

H2CO3

H+

Na+

NH3

H+

NH3

Na+

NH4+

Glutamine

α-Ketoglutarate2-

Glucose orCO2 + H2O

A cell model for renal synthesis and excre-

tion of ammonia. Ammonium ions are formedfrom glutamine in the cell and are secreted into the tubular urine(top). H� from H2CO3 (bottom) is consumed when �-ketoglu-tarate is converted into glucose or CO2 and H2O. New HCO3

is added to the peritubular capillary blood—1 mEq for each mEqof NH4

� excreted in the urine.

FIGURE 25.10

Page 12: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

eral days. Enhanced renal ammonia synthesis and excretionis a lifesaving adaptation because it allows the kidneys toremove large H� excesses and add more new HCO3

� tothe blood. Also, the excreted NH4

� can substitute in theurine for Na� and K�, diminishing the loss of these cations.With severe metabolic acidosis, ammonia excretion mayincrease almost 10-fold.

Several Factors Influence Renal Excretion

of Hydrogen Ions

Several factors influence the renal excretion of H�, includ-ing intracellular pH, arterial blood PCO2, carbonic anhy-drase activity, Na� reabsorption, plasma [K�], and aldos-terone (Fig. 25.11).

Intracellular pH. The pH in kidney tubule cells is a keyfactor influencing the secretion and, therefore, the excretionof H�. A fall in pH (increased [H�]) enhances H� secretion.A rise in pH (decreased [H�]) lowers H� secretion.

Arterial Blood PCO2. An increase in PCO2 increases theformation of H� from H2CO3, leading to enhanced renalH� secretion and excretion—a useful compensation for anycondition in which the blood contains too much H2CO3.(This will be discussed later, when we consider respiratoryacidosis.) A decrease in PCO2 results in lowered H� secretionand, consequently, less complete reabsorption of filteredHCO3

� and a loss of base in the urine (a useful compensa-tion for respiratory alkalosis, also discussed later).

Carbonic Anhydrase Activity. The enzyme carbonic an-hydrase catalyzes two key reactions in urinary acidification:

1) Hydration of CO2 in the cells, forming H2CO3 andyielding H� for secretion

2) Dehydration of H2CO3 to H2O and CO2 in theproximal tubule lumen, an important step in the reabsorp-tion of filtered HCO3

If carbonic anhydrase is inhibited (usually by a drug),large amounts of filtered HCO3

� may escape reabsorption.This situation leads to a fall in blood pH.

Sodium Reabsorption. Na� reabsorption is closelylinked to H� secretion. In the proximal tubule, the two ionsare directly linked, both being transported by the Na�/H�

exchanger in the luminal plasma membrane. The relation isless direct in the collecting ducts. Enhanced Na� reabsorp-tion in the ducts leads to a more negative intraluminal elec-trical potential, which favors H� secretion by its electro-genic H�-ATPase. The avid renal reabsorption of Na�

observed in states of volume depletion is accompanied by aparallel rise in urinary H� excretion.

Plasma Potassium Concentration. Changes in plasma[K�] influence the renal excretion of H�. A fall in plasma[K�] favors the movement of K� from body cells into in-terstitial fluid (or blood plasma) and a reciprocal move-ment of H� into cells. In the kidney tubule cells, thesemovements lower intracellular pH and increase H� se-cretion. K� depletion also stimulates ammonia synthesisby the kidneys. The result is the complete reabsorptionof filtered HCO3

� and the enhanced generation of newHCO3

� as more titratable acid and ammonia are ex-creted. Consequently, hypokalemia (or a decrease inbody K� stores) leads to increased plasma [HCO3

�](metabolic alkalosis). Hyperkalemia (or excess K� in thebody) results in the opposite changes: an increase in in-tracellular pH, decreased H� secretion, incomplete reab-sorption of filtered HCO3

�, and a fall in plasma[HCO3

�] (metabolic acidosis).

Aldosterone. Aldosterone stimulates the collecting ductsto secrete H� by three actions:

1) It directly stimulates the H�-ATPase in collectingduct �-intercalated cells.

2) It enhances collecting duct Na� reabsorption, whichleads to a more negative intraluminal potential and, conse-quently, promotes H� secretion by the electrogenic H�-ATPase.

3) It promotes K� secretion. This response leads to hy-pokalemia, which increases renal H� secretion.

Hyperaldosteronism results in enhanced renal H� ex-cretion and an alkaline blood pH; the opposite occurs withhypoaldosteronism.

pH gradient. The secretion of H� by the kidney tubulesand collecting ducts is gradient-limited. The collectingducts cannot lower the urine pH below 4.5, correspondingto a urine/plasma [H�] gradient of 10�4.5/10�7.4 or 800/1when the plasma pH is 7.4. If more buffer base (NH3,HPO4

2–) is available in the urine, more H� can be secretedbefore the limiting gradient is reached. In some kidneytubule disorders, the secretion of H� is gradient-limited(see Clinical Focus Box 25.1).

CHAPTER 25 Acid-Base Balance 437

Peritubularblood

Tubularepithelium

Tubularurine

H+

H+

CO2

HCO3-

H+-ATPase

Increasedsodium

reabsorption

IncreasedPCO2

Decreasedintracellular

pHH+

Carbonic anhydraseactivity

CACO2 H2O+

Na+

H2CO3

HCO3- H+

Na+

Increasedplasma

aldosterone

H+

K+DecreasedK+

Factors leading to increased H� secretion

by the kidney tubule epithelium. (See textfor details.)

FIGURE 25.11

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438 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

REGULATION OF INTRACELLULAR PH

The intracellular and extracellular fluids are linked by ex-changes across plasma membranes of H�, HCO3

�, variousacids and bases, and CO2. By stabilizing ECF pH, the bodyhelps to protect intracellular pH.

If H� ions were passively distributed across plasmamembranes, intracellular pH would be lower than what isseen in most body cells. In skeletal muscle cells, for exam-ple, we can calculate from the Nernst equation (see Chap-ter 2) and a membrane potential of �90 mV that cytosolicpH should be 5.9 if ECF pH is 7.4; actual measurements,however, indicate a pH of 6.9. From this discrepancy, twoconclusions are clear: H� ions are not at equilibrium acrossthe plasma membrane, and the cell must use active mecha-nisms to extrude H�.

Cells are typically threatened by acidic metabolic end-products and by the tendency for H� to diffuse into the celldown the electrical gradient (Fig. 25.12). H� is extruded byNa�/H� exchangers, which are present in nearly all bodycells. Five different isoforms of these exchangers (desig-nated NHE1, NHE2, etc.), with different tissue distribu-tions, have been identified. These transporters exchangeone H� for one Na� and, therefore, function in an electri-cally neutral fashion. Active extrusion of H� keeps the in-ternal pH within narrow limits.

The activity of the Na�/H� exchanger is regulated byintracellular pH and a variety of hormones and growth fac-tors (Fig. 25.13). Not surprisingly, an increase in intracellu-

CLINICAL FOCUS BOX 25.1

Renal Tubular Acidosis

Renal tubular acidosis (RTA) is a group of kidney disor-ders characterized by chronic metabolic acidosis, a normalplasma anion gap, and the absence of renal failure. Thekidneys show inadequate H� secretion by the distalnephron, excessive excretion of HCO3

�, or reduced excre-tion of NH4

�.In classic type 1 (distal) RTA, the ability of the col-

lecting ducts to lower urine pH is impaired. This conditioncan be caused by inadequate secretion of H� (defectiveH�-ATPase or H�/K�-ATPase) or abnormal leakiness ofthe collecting duct epithelium so that secreted H� ionsdiffuse back from lumen to blood. Because the urine pH isinappropriately high, titratable acid excretion is dimin-ished and trapping of ammonia in the urine (as NH4

�) isdecreased. Type 1 RTA may be the result of an inheriteddefect, autoimmune disease, treatment with lithium orthe antibiotic amphotericin B, or the result of diseases ofthe kidney medulla. A diagnosis of this form of RTA is es-tablished by challenging the subject with a standard oraldose of NH4Cl and measuring the urine pH for the nextseveral hours. This results in a urine pH below 5.0 inhealthy people. In subjects with type 1 RTA, however,urine pH will not decrease below 5.5. Treatment of type 1RTA involves daily administration of modest amounts ofalkali (HCO3

�, citrate) sufficient to cover daily metabolicacid production.

In type 2 (proximal) RTA, HCO3� reabsorption by the

proximal tubule is impaired, leading to excessive losses ofHCO3

� in the urine. As a consequence, the plasma [HCO3�]

falls and chronic metabolic acidosis ensues. In the newsteady state, the tubules are able to reabsorb the filteredHCO3

� load more completely because the filtered load isreduced. The distal nephron is no longer overwhelmed byHCO3

� and the urine pH is acidic. In type 2 RTA, the ad-ministration of an NH4Cl challenge results in a urine pH be-low 5.5. This disorder may be inherited, may be associatedwith several acquired conditions that result in a general-ized disorder of proximal tubule transport, or may resultfrom the inhibition of proximal tubule carbonic anhydraseby drugs such as acetazolamide. Treatment requires thedaily administration of large amounts of alkali becausewhen the plasma [HCO3

�] is raised, excessive urinary ex-cretion of filtered HCO3

� occurs.Type 4 RTA (there is no type 3 RTA) is also known as

hyperkalemic distal RTA. Collecting duct secretion ofboth K� and H� is reduced, explaining the hyperkalemiaand metabolic acidosis. Hyperkalemia reduces renal am-monia synthesis, resulting in reduced net acid excretionand a fall in plasma [HCO3

�]. The urine pH can go below5.5 after an NH4Cl challenge because there is little ammo-nia in the urine to buffer secreted H�. The underlying dis-order is a result of inadequate production of aldosterone orimpaired aldosterone action. Treatment of type 4 RTA re-quires lowering the plasma [K�] to normal; if this therapyis successful, alkali may not be needed.

Na+

CO2

+-

Metabolism

H+

H+

H+

H+

Na+

HCO3-

Cl-

CO2

Cell acid-base balance. Body cells usuallymaintain a constant intracellular pH. The cell is

acidified by the production of H� from metabolism and the in-flux of H� from the ECF (favored by the inside negative plasmamembrane potential). To maintain a stable intracellular pH, thecell must extrude hydrogen ions at a rate matching their input.Many cells also possess various HCO3

� transporters (not de-picted), which defend against excess acid or base.

FIGURE 25.12

Page 14: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

lar [H�] stimulates the exchanger but not only because ofmore substrate (H�) for the exchanger. H� also stimulatesthe exchanger by protonating an activator site on the cyto-plasmic side of the exchanger, making the exchanger moreeffective in dealing with the threat of intracellular acidosis.Many hormones and growth factors, via intracellular sec-ond messengers, activate various protein kinases that stim-ulate or inhibit the Na�/H� exchanger. In this way, theyproduce changes in intracellular pH, which may lead tochanges in cell activity.

Besides extruding H�, the cell can deal with acids andbases in other ways. In some cells, various HCO3

� trans-porting systems (e.g., Na�-dependent and Na�-independ-ent Cl�/HCO3

� exchangers) may be present in plasmamembranes. These exchangers may be activated bychanges in intracellular pH. Cells have large stores of pro-tein and organic phosphate buffers, which can bind or re-lease H�. Various chemical reactions in cells can also useup or release H�. For example, the conversion of lactic acidto CO2 and water to glucose effectively disposes of acid. Inaddition, various cell organelles may sequester H�. For ex-ample, H�-ATPase in endosomes and lysosomes pumpsH� out of the cytosol into these organelles. In summary,ion transport, buffering mechanisms, and metabolic reac-tions all ensure a relatively stable intracellular pH.

DISTURBANCES OF ACID-BASE BALANCE

Table 25.2 lists the normal values for the pH (or [H�]),PCO2, and [HCO3

�] of arterial blood plasma. A blood pHof less than 7.35 ([H�] 45 nmol/L) indicates acidemia. Ablood pH above 7.45 ([H�] 35 nmol/L) indicates alka-lemia. The range of pH values compatible with life is ap-proximately 6.8 to 7.8 ([H�] � 160 to 16 nmol/L).

Four simple acid-base disturbances may lead to an ab-normal blood pH: respiratory acidosis, respiratory alkalo-sis, metabolic acidosis, and metabolic alkalosis. The word“simple” indicates a single primary cause for the distur-

bance. Acidosis is an abnormal process that tends to pro-duce acidemia. Alkalosis is an abnormal process that tendsto produce alkalemia. If there is too much or too little CO2,a respiratory disturbance is present. If the problem is toomuch or too little HCO3

�, a metabolic (or nonrespiratory)disturbance of acid-base balance is present. Table 25.3summarizes the changes in blood pH (or [H�]), plasma[HCO3

�], and PCO2 that occur in each of the four simpleacid-base disturbances.

In considering acid-base disturbances, it is helpful to re-call the Henderson-Hasselbalch equation forHCO3

�/CO2:

pH � 6.10 � log (25)

If the primary problem is a change in [HCO3�] or PCO2,

the pH can be brought closer to normal by changing theother member of the buffer pair in the same direction. For ex-ample, if PCO2 is primarily decreased, a decrease in plasma[HCO3

�] will minimize the change in pH. In various acid-base disturbances, the lungs adjust the blood PCO2 and thekidneys adjust the plasma [HCO3

�] to reduce departuresof pH from normal; these adjustments are called compen-sations (Table 25.4). Compensations generally do notbring about normal blood pH.

Respiratory Acidosis Results From an

Accumulation of Carbon Dioxide

Respiratory acidosis is an abnormal process characterizedby CO2 accumulation. The CO2 build-up pushes the fol-lowing reactions to the right:

CO2 � H2O H2CO3 H� � HCO3� (26)

Blood [H2CO3] increases, leading to an increase in [H�]or a fall in pH. Respiratory acidosis is usually caused by afailure to expire metabolically produced CO2 at an ade-quate rate, leading to accumulation of CO2 in the bloodand a fall in blood pH. This disturbance may be a result ofa decrease in overall alveolar ventilation (hypoventilation)or, as occurs commonly in lung disease, a mismatch be-tween ventilation and perfusion. Respiratory acidosis alsooccurs if a person breathes CO2-enriched air.

Chemical Buffering. In respiratory acidosis, more than95% of the chemical buffering occurs within cells. The cells

[HCO3�]

��0.03 PCO2

CHAPTER 25 Acid-Base Balance 439

Na�/H�

exchanger

The plasma membrane Na�/H� exchanger.This exchanger plays a key role in regulating

intracellular pH in most body cells and is activated by a decreasein cytoplasmic pH. Many hormones and growth factors, actingvia intracellular second messengers and protein kinases, can in-crease (�) or decrease (�) the activity of the exchange.

FIGURE 25.13

TABLE 25.2Normal Arterial Blood Plasma

Acid-Base Values

Mean Rangea

pH 7.40 7.35–7.45[H�], nmol/L 40 45–35PCO2, mm Hg 40 35–45[HCO3

�], mEq/L 24 22–26

a The range extends from 2 standard deviations below to 2 standarddeviations above the mean and encompasses 95% of the healthypopulation.

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440 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

contain many proteins and organic phosphates that canbind H�. For example, hemoglobin (Hb) in red blood cellscombines with H� from H2CO3, minimizing the increasein free H�. Recall from Chapter 21 the buffering reaction:

H2CO3 � HbO2� HHb � O2 � HCO3

� (27)

This reaction raises the plasma [HCO3�]. In acute respira-

tory acidosis, such chemical buffering processes in the bodylead to an increase in plasma [HCO3

�] of about 1 mEq/L foreach 10 mm Hg increase in PCO2 (see Table 25.4). Bicar-bonate is not a buffer for H2CO3 because the reaction

H2CO3 � HCO3� HCO3

� � H2CO3 (28)

is simply an exchange reaction and does not affect the pH.An example illustrates how chemical buffering reduces a

fall in pH during respiratory acidosis. Suppose PCO2 in-creased from a normal value of 40 mm Hg to 70 mm Hg([CO2(d)] � 2.l mmol/L). If there were no body buffer basesthat could accept H� from H2CO3 (i.e., if there was nomeasurable increase in [HCO3

�]), the resulting pH wouldbe 7.16:

pH � 6.10 � log � 7.16 (29)

In acute respiratory acidosis, a 3 mEq/L increase inplasma [HCO3

�] occurs with a 30 mm Hg rise in PCO2 (seeTable 25.4). Therefore, the pH is 7.21:

pH � 6.10 � log � 7.21 (30)

The pH of 7.21 is closer to a normal pH because bodybuffer bases (mainly intracellular buffers) such as proteinsand phosphates combined with H� ions liberated fromH2CO3.

Respiratory Compensation. Respiratory acidosis pro-duces a rise in PCO2 and a fall in pH and is often associatedwith hypoxia. These changes stimulate breathing (seeChapter 22) and diminish the severity of the acidosis. Inother words, a person would be worse off if the respiratorysystem did not reflexively respond to the abnormalities inblood PCO2, pH, and PO2.

[24 � 3]�

[2.1]

[24]�[2.1]

Renal Compensation. The kidneys compensate for respi-ratory acidosis by adding more H� to the urine and addingnew HCO3

� to the blood. The increased PCO2 stimulatesrenal H� secretion, which allows the reabsorption of all fil-tered HCO3

�. Excess H� is excreted as titratable acid andNH4

�; these processes add new HCO3� to the blood,

causing plasma [HCO3�] to rise. This compensation takes

several days to fully develop.With chronic respiratory acidosis, plasma [HCO3

�] in-creases, on average, by 4 mEq/L for each 10 mm Hg rise inPCO2 (see Table 25.4). This rise exceeds that seen withacute respiratory acidosis because of the renal addition ofHCO3

� to the blood. One would expect a person withchronic respiratory acidosis and a PCO2 of 70 mm Hg tohave an increase in plasma HCO3

� of 12 mEq/L. Theblood pH would be 7.33:

pH � 6.10 � log �[24

[2�

.1]12]

� � 7.33 (31)

TABLE 25.3 Directional Changes in Arterial Blood Plasma Values in the Four Simple Acid-Base Disturbancesa

Arterial Plasma

Disturbance pH [H�] [HCO3�] PCO2 Compensatory Response

Respiratory acidosis ↓ ↑ ↑ ⇑ Kidneys increase H� excretionRespiratory alkalosis ↑ ↓ ↓ ⇓ Kidneys increase HCO3

�excretionMetabolic acidosis ↓ ↑ ⇓ ↓ Alveolar hyperventilation; kidneys increase

H� excretionMetabolic alkalosis ↑ ↓ ⇑ ↑ Alveolar hypoventilation; kidneys increase

HCO3�excretion

a Heavy arrows indicate the main effect.

TABLE 25.4 Compensatory Responses in Acid-Base

Disturbancesa

Respiratory acidosisAcute 1 mEq/L increase in plasma [HCO3

�]for each 10 mm Hg increase in PCO2

b

Chronic 4 mEq/L increase in plasma [HCO3�]

for each 10 mm Hg increase in PCO2c

Respiratory alkalosisAcute 2 mEq/L decrease in plasma [HCO3

�]for each 10 mm Hg decrease in PCO2

d

Chronic 4 mEq/L decrease in plasma [HCO3�]

for each 10 mm Hg decrease in PCO2d

Metabolic acidosis 1.3 mm Hg decrease in PCO2 for each1 mEq/L decrease in plasma [HCO3

�]d

Metabolic alkalosis 0.7 mm Hg increase in PCO2 for each1 mEq/L increase in plasma [HCO3

�]d

From Valtin H, Gennari FJ. Acid-Base Disorders. Basic Concepts andClinical Management. Boston: Little, Brown, 1987.a Empirically determined average changes measured in people withsimple acid-base disorders.b This change is primarily a result of chemical buffering.c This change is primarily a result of renal compensation.d This change is a result of respiratory compensation.

Page 16: 25 CHAPTER Acid-Base Balance - KSUfac.ksu.edu.sa/sites/default/files/smch25.pdf426 Acid-Base Balance 25 George A. Tanner, Ph.D. CHAPTER A REVIEW OF ACID-BASE CHEMISTRY PRODUCTION AND

With chronic respiratory acidosis, time for renal com-pensation is allowed, so blood pH (in this example, 7.33) ismuch closer to normal than is observed during acute respi-ratory acidosis (pH 7.21).

Respiratory Alkalosis Results From an

Excessive Loss of Carbon Dioxide

Respiratory alkalosis is most easily understood as theopposite of respiratory acidosis; it is an abnormalprocess causing the loss of too much CO2. This losscauses blood [H2CO3] and, thus, [H�] to fall (pH rises).Alveolar hyperventilation causes respiratory alkalosis.Metabolically produced CO2 is flushed out of the alveo-lar spaces more rapidly than it is added by the pul-monary capillary blood. This situation causes alveolarand arterial PCO2 to fall. Hyperventilation and respira-tory alkalosis can be caused by voluntary effort, anxiety,direct stimulation of the medullary respiratory center bysome abnormality (e.g., meningitis, fever, aspirin intox-ication), or hypoxia caused by severe anemia or high al-titude.

Chemical Buffering. As with respiratory acidosis, dur-ing respiratory alkalosis more than 95% of chemicalbuffering occurs within cells. Cell proteins and organicphosphates liberate H� ions, which are added to theECF and lower the plasma [HCO3

�], reducing the alka-line shift in pH.

With acute respiratory alkalosis, plasma [HCO3�] falls

by about 2 mEq/L for each 10 mm Hg drop in PCO2 (seeTable 25.4). For example, if PCO2 drops from 40 to 20 mmHg ([CO2(d)] � 0.6 mmol/L) plasma [HCO3

�] falls by 4mEq/L, and the pH will be 7.62:

pH � 6.10 � log �[2

[40�

.6]4]

� � 7.62 (32)

If plasma [HCO3�] had not changed, the pH would

have been 7.70:

pH � 6.10 � log �[[02.46]]

� � 7.70 (33)

Respiratory Compensation. Although hyperventilationcauses respiratory alkalosis, hyperventilation also causeschanges (a fall in PCO2 and a rise in blood pH) that in-hibit ventilation and, therefore, limit the extent of hy-perventilation.

Renal Compensation. The kidneys compensate for respi-ratory alkalosis by excreting HCO3

� in the urine, thereby,getting rid of base. A reduced PCO2 reduces H� secretionby the kidney tubule epithelium. As a result, some of the fil-tered HCO3

� is not reabsorbed. When the urine becomesmore alkaline, titratable acid excretion vanishes and littleammonia is excreted. The enhanced output of HCO3

causes plasma [HCO3�] to fall.

Chronic respiratory alkalosis is accompanied by a 4mEq/L fall in plasma [HCO3

�] for each l0 mm Hg drop inPCO2 (see Table 25.4). For example, in a person with

chronic hyperventilation and a PCO2 of 20 mm Hg, theblood pH is

pH � 6.10 � log �[2

[40�

.6]8]

� � 7.53 (34)

This pH is closer to normal than the pH of 7.62 of acuterespiratory alkalosis. The difference between the two situ-ations is largely a result of renal compensation.

Metabolic Acidosis Results From a Gain of

Noncarbonic Acid or a Loss of Bicarbonate

Metabolic acidosis is an abnormal process characterizedby a gain of acid (other than H2CO3) or a loss of HCO3

�.Either causes plasma [HCO3

�] and pH to fall. If a strongacid is added to the body, the reactions

H� � HCO3� H2CO3 H2O � CO2 (35)

are pushed to the right. The added H� consumesHCO3

�. If a lot of acid is infused rapidly, PCO2 rises, asthe equation predicts. This increase occurs only tran-siently, however, because the body is an open system,and the lungs expire CO2 as it is generated. PCO2 actuallyfalls below normal because an acidic blood pH stimulatesventilation (see Fig. 25.3).

Many conditions can produce metabolic acidosis, in-cluding renal failure, uncontrolled diabetes mellitus, lac-tic acidosis, the ingestion of acidifying agents such asNH4Cl, abnormal renal excretion of HCO3

�, and diar-rhea. In renal failure, the kidneys cannot excrete H� fastenough to keep up with metabolic acid production and,in uncontrolled diabetes mellitus, the production of ke-tone body acids increases. Lactic acidosis results fromtissue hypoxia. Ingested NH4Cl is converted into ureaand a strong acid, HCl, in the liver. Diarrhea causes aloss of alkaline intestinal fluids. Clinical Focus Box 25.2discusses the metabolic acidosis seen in uncontrolled di-abetes mellitus.

Chemical Buffering. Excess acid is chemically buffered inextracellular and intracellular fluids and bone. In metabolicacidosis, roughly half the buffering occurs in cells andbone. HCO3

� is the principal buffer in the ECF.

Respiratory Compensation. The acidic blood pH stimu-lates the respiratory system to lower blood PCO2. This actionlowers blood [H2CO3] and tends to alkalinize the blood,opposing the acidic shift in pH. Metabolic acidosis is ac-companied on average by a l.3 mm Hg fall in PCO2 for eachl mEq/L drop in plasma [HCO3

�] (see Table 25.4). Suppose,for example, the infusion of a strong acid causes the plasma[HCO3

�] to drop from 24 to l2 mEq/L. If there was no res-piratory compensation and the PCO2 did not change from itsnormal value of 40 mm Hg, the pH would be 7.10:

pH � 6.10 � log �[[11.22]]

� � 7.10 (36)

CHAPTER 25 Acid-Base Balance 441

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442 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

With respiratory compensation, the PCO2 falls by 16mm Hg (12 � 1.3) to 24 mm Hg ([CO2(d)] � 0.72 mmol/L)and pH is 7.32:

pH � 6.10 � log �[0[1.722]]

� � 7.32 (37)

This value is closer to normal than a pH of 7.10. The res-piratory response develops promptly (within minutes) andis maximal after 12 to 24 hours.

Renal Compensation. The kidneys respond to metabolicacidosis by adding more H� to the urine. Since the plasma[HCO3

�] is primarily lowered, the filtered load of HCO3�

drops, and the kidneys can accomplish the complete reab-sorption of filtered HCO3

� (see Fig. 25.7). More H� is ex-creted as titratable acid and NH4

�. With chronic meta-bolic acidosis, the kidneys make more ammonia. Thekidneys can, therefore, add more new HCO3

� to theblood, to replace lost HCO3

�. If the underlying cause ofmetabolic acidosis is corrected, then healthy kidneys cancorrect the blood pH in a few days.

The Plasma Anion Gap Is Calculated From

Na�, Cl�, and HCO3� Concentrations

The anion gap is a useful concept, especially when trying todetermine the possible cause of a metabolic acidosis. In anybody fluid, the sums of the cations and anions are equal be-cause solutions are electrically neutral. For blood plasma,we can write

� cations � � anions (38)or

[Na�] � [unmeasured cations] � [Cl�]� [HCO3

�] � [unmeasured anions] (39)

The unmeasured cations include K�, Ca2�, and Mg2�

ions and, because these are present at relatively low con-centrations (compared to Na�) and are usually fairly con-stant, we choose to neglect them. The unmeasured anionsinclude plasma proteins, sulfate, phosphate, citrate, lactate,and other organic anions. If we rearrange the above equa-tion, we get

[unmeasured anions] or anion gap �[Na�] � [Cl�] � [HCO3

�] (40)

CLINICAL FOCUS BOX 25.2

Metabolic Acidosis in Diabetes Mellitus

Diabetes mellitus is a common disorder characterized byan insufficient secretion of insulin or insulin-resistance bythe major target tissues (skeletal muscle, liver, andadipocytes). A severe metabolic acidosis may develop inuncontrolled diabetes mellitus.

Acidosis occurs because insulin deficiency leads to de-creased glucose utilization, a diversion of metabolism to-ward the utilization of fatty acids, and an overproduction ofketone body acids (acetoacetic acid and �-hydroxybutyricacids). Ketone body acids are fairly strong acids (pKa 4 to5); they are neutralized in the body by HCO3

� and otherbuffers. Increased production of these acids leads to a fallin plasma [HCO3

�], an increase in plasma anion gap, and afall in blood pH (acidemia).

Severe acidemia, whatever its cause, has many adverseeffects on the body. It impairs myocardial contractility, re-sulting in a decrease in cardiac output. It causes arteriolar di-lation, which leads to a fall in arterial blood pressure. He-patic and renal blood flows are decreased. Reentrantarrhythmias and a decreased threshold for ventricular fibril-lation can occur. The respiratory muscles show decreasedstrength and fatigue easily. Metabolic demands are in-creased due, in part, to activation of the sympathetic nerv-ous system, but at the same time anaerobic glycolysis andATP synthesis are reduced by acidemia. Hyperkalemia is fa-vored and protein catabolism is enhanced. Severe acidemiacauses impaired brain metabolism and cell volume regula-tion, leading to progressive obtundation and coma.

An increased acidity of the blood stimulates pulmonaryventilation, resulting in a compensatory lowering of alveo-

lar and arterial blood PCO2. The consequent reduction inblood [H2CO3] acts to move the blood pH back toward nor-mal. The labored, deep breathing that accompanies severeuncontrolled diabetes is called Kussmaul’s respiration.

The kidneys compensate for metabolic acidosis by re-absorbing all the filtered HCO3

�. They also increase the ex-cretion of titratable acid, part of which is comprised of ke-tone body acids. But these acids can only be partiallytitrated to their acid form in the urine because the urine pHcannot go below 4.5. Therefore, ketone body acids are ex-creted mostly in their anionic form; because of the re-quirement of electroneutrality in solutions, increased uri-nary excretion of Na� and K� results.

An important compensation for the acidosis is in-creased renal synthesis and excretion of ammonia. Thisadaptive response takes several days to fully develop, butit allows the kidneys to dispose of large amounts of H� inthe form NH4

�. The NH4

� in the urine can replace Na� andK� ions, resulting in conservation of these valuablecations.

The severe acidemia, electrolyte disturbances, and vol-ume depletion that accompany uncontrolled diabetes mel-litus may be fatal. Addressing the underlying cause, ratherthan just treating the symptoms best achieves correctionof the acid-base disturbance. Therefore, the administrationof a suitable dose of insulin is usually the key element oftherapy. In some patients with marked acidemia (pH 7.10), NaHCO3 solutions may be infused intravenously tospeed recovery, but this does not correct the underlyingmetabolic problem. Losses of Na�, K�, and water shouldbe replaced.

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In a healthy person, the anion gap falls in the range of 8to 14 mEq/L. For example, if plasma [Na�] is 140 mEq/L,[Cl�] is 105 mEq/L, and [HCO3

�] is 24 mEq/L, the aniongap is 11 mEq/L. If an acid such as lactic acid is added toplasma, the reaction lactic acid � HCO3

� lactate� �H2O � CO2 will be pushed to the right. Consequently, theplasma [HCO3

�] will be decreased and because the [Cl�] isnot changed, the anion gap will be increased. The unmea-sured anion in this case is lactate. In several types of meta-bolic acidosis, the low blood pH is accompanied by a highanion gap (Table 25.5). (These can be remembered from themnemonic MULEPAKS formed from the first letters of thislist.) In other types of metabolic acidosis, the low blood pHis accompanied by a normal anion gap (see Table 25.5). Forexample, with diarrhea and a loss of alkaline intestinal fluid,plasma [HCO3

�] falls but plasma [Cl�] rises, and the twochanges counterbalance each other so the anion gap is un-changed. Again, the chief value of the anion gap concept isthat it allows a clinician to narrow down possible explana-tions for metabolic acidosis in a patient.

Metabolic Alkalosis Results From a Gain

of Strong Base or Bicarbonate or a Loss

of Noncarbonic Acid

Metabolic alkalosis is an abnormal process characterizedby a gain of a strong base or HCO3

� or a loss of an acid(other than carbonic acid). Plasma [HCO3

�] and pH rise;PCO2 rises because of respiratory compensation. Thesechanges are opposite to those seen in metabolic acidosis(see Table 25.3). A variety of situations can produce meta-bolic alkalosis, including the ingestion of antacids, vomit-ing of gastric acid juice, and enhanced renal H� loss (e.g.,as a result of hyperaldosteronism or hypokalemia). ClinicalFocus Box 25.3 discusses the metabolic alkalosis producedby vomiting of gastric juice.

Chemical Buffering. Chemical buffers in the body limitthe alkaline shift in blood pH by releasing H� as they are

titrated in the alkaline direction. About one third of thebuffering occurs in cells.

Respiratory Compensation. The respiratory compensa-tion for metabolic alkalosis is hypoventilation. An alkalineblood pH inhibits ventilation. Hypoventilation raises theblood PCO2 and [H2CO3], reducing the alkaline shift inpH. A l mEq/L rise in plasma [HCO3

�] caused by meta-bolic alkalosis is accompanied by a 0.7 mm Hg rise inPCO2 (see Table 25.4). If, for example, the plasma[HCO3

�] rose to 40 mEq/L, what would the plasma pHbe with and without respiratory compensation? With res-piratory compensation, the PCO2 should rise by 11.2 mmHg (0.7 � 16) to 51.2 mm Hg ([CO2(d)] � 1.54 mmol/L).The pH is 7.51:

pH � 6.10 � log �[1[4.504]]

� � 7.51 (41)

Without respiratory compensation, the pH would be7.62:

pH � 6.10 � log �[[14.02]]

� � 7.62 (42)

Respiratory compensation for metabolic alkalosis is lim-ited because hypoventilation leads to hypoxia and CO2 re-tention, and both increase breathing.

Renal Compensation. The kidneys respond to meta-bolic alkalosis by lowering the plasma [HCO3

�]. Theplasma [HCO3

�] is primarily raised and more HCO3� is

filtered than can be reabsorbed (see Fig. 25.7); in addi-tion, HCO3

� is secreted in the collecting ducts. Both ofthese changes lead to increased urinary [HCO3

�] excre-tion. If the cause of the metabolic alkalosis is corrected,the kidneys can often restore the plasma [HCO3

�] andpH to normal in a day or two.

CHAPTER 25 Acid-Base Balance 443

TABLE 25.5 High and Normal Anion Gap Metabolic Acidosis

Condition Explanation

High anion gap metabolicacidosisMethanol intoxication Methanol metabolized to formic acidUremia Sulfuric, phosphoric, uric, and hippuric acids retained due to renal failureLactic acid Lactic acid buffered by HCO3

� and accumulates as lactateEthylene glycol intoxication Ethylene glycol metabolized to glyoxylic, glycolic, and oxalic acidsp-Aldehyde intoxication p-Aldehyde metabolized to acetic and chloroacetic acidsKetoacidosis Production of �-hydroxybutyric and acetoacetic acidsSalicylate intoxication Impaired metabolism leads to production of lactic acid and ketone body acids; accumulation of salicylateNormal anion gap metabolic acidosisDiarrhea Loss of HCO3

� in stool; kidneys conserve Cl�

Renal tubular acidosis Loss of HCO3� in urine or inadequate excretion of H�; kidneys conserve Cl�

Ammonium chloride ingestion NH4� is converted to urea in liver, a process that consumes HCO3

�; excess Cl� is ingested

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444 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

Clinical Evaluation of Acid-Base Disturbances

Requires a Comprehensive Study

Acid-base data should always be interpreted in the contextof other information about a patient. A complete historyand physical examination provide important clues to possi-ble reasons for an acid-base disorder.

To identify an acid-base disturbance from laboratoryvalues, it is best to look first at the pH. A low blood pH in-dicates acidosis; a high blood pH indicates alkalosis. If aci-dosis is present, for example, it could be either respiratoryor metabolic. A low blood pH and elevated PCO2 point to

respiratory acidosis; a low pH and low plasma [HCO3�] in-

dicate metabolic acidosis. If alkalosis is present, it could beeither respiratory or metabolic. A high blood pH and lowplasma PCO2 indicate respiratory alkalosis; a high blood pHand high plasma [HCO3

�] indicate metabolic alkalosis.Whether the body is making an appropriate response for

a simple acid-base disorder can be judged from the valuesin Table 25.4. Inappropriate values suggest that more thanone acid-base disturbance may be present. Patients mayhave two or more of the four simple acid-base disturbancesat the same time; in which case, they have a mixed acid-base disturbance.

CLINICAL FOCUS BOX 25.3

Vomiting and Metabolic Alkalosis

Vomiting of gastric acid juice results in metabolic alka-losis and fluid and electrolyte disturbances. Gastric acidjuice contains about 0.1 M HCl. The acid is secreted bystomach parietal cells; these cells have an H�/K�-ATPasein their luminal plasma membrane and a Cl�/HCO3

� ex-changer in their basolateral plasma membrane. When HClis secreted into the stomach lumen and lost to the outside,there is a net gain of HCO3

� in the blood plasma and nochange in the anion gap. The HCO3

�, in effect, replaces lostplasma Cl�.

Ventilation is inhibited by the alkaline blood pH, result-ing in a rise in PCO2. This respiratory compensation for themetabolic alkalosis, however, is limited because hypoven-tilation leads to a rise in PCO2 and a fall in PO2, both of whichstimulate breathing.

The logical renal compensation for metabolic alkalosisis enhanced excretion of HCO3

�. In people with persistentvomiting, however, the urine is sometimes acidic and renalHCO3

�reabsorption is enhanced, maintaining an elevatedplasma [HCO3

�]. This situation occurs because vomiting isaccompanied by losses of ECF and K�. Fluid loss leads to adecrease in effective arterial blood volume and engage-ment of mechanisms that reduce Na� excretion, such asdecreased GFR and increased plasma renin, angiotensin,and aldosterone levels (see Chapter 24). Aldosterone stim-ulates H� secretion by collecting duct �-intercalated cells.

Renal tubular Na�/H� exchange is stimulated by volumedepletion because the tubules reabsorb Na� more avidlythan usual. With more H� secretion, more new HCO3

� isadded to the blood. The kidneys reabsorb filtered HCO3

completely, even though plasma HCO3� level is elevated,

and maintain the metabolic alkalosis.Vomiting results in K� depletion because of a loss of K�

in the vomitus, decreased food intake and, most importantquantitatively, enhanced renal K� excretion. Extracellularalkalosis results in a shift of K� into cells (including renalcells) and, thereby, promotes K� secretion and excretion.Elevated plasma aldosterone levels also favor K� loss inthe urine.

Treatment of metabolic alkalosis primarily depends oneliminating the cause of vomiting. Correction of the alka-losis by administering an organic acid, such as lactic acid,does not make sense because this acid would simply beconverted to CO2 and H2O; this approach also does notaddress the Cl� deficit. The ECF volume depletion and theCl� and K� deficits can be corrected by administering iso-tonic saline and appropriate amounts of KCl. Because re-placement of Cl� is a key component of therapy, this typeof metabolic alkalosis is said to be “chloride-responsive.”After Na�, Cl�, water, and K� deficits have been replaced,excess HCO3

� (accompanied by surplus Na�) will be ex-creted in the urine, and the kidneys will return blood pHto normal.

DIRECTIONS: Each of the numbereditems or incomplete statements in thissection is followed by answers or bycompletions of the statement. Select theONE lettered answer or completion that isBEST in each case.

1. If the pKa of NH4� is 9.0, the ratio of

NH3 to NH4� in a urine sample with a

pH of 6.0 is(A) 1:3(B) 3:1(C) 3:2(D) 1:1,000

(E) 1,000:12. An arterial blood sample taken from a

patient has a pH of 7.32 ([H�] � 48nmol/L) and PCO2 of 24 mm Hg. Whatis the plasma [HCO3

�]?(A) 6 mEq/L(B) 12 mEq/L(C) 20 mEq/L(D) 24 mEq/L(E) 48 mEq/L

3. Which segment can establish thesteepest pH gradient (tubular fluid-to-blood)?(A) Proximal convoluted tubule

(B) Thin ascending limb(C) Thick ascending limb(D) Distal convoluted tubule(E) Collecting duct

4. Most of the hydrogen ions secreted bythe kidney tubules are(A) Consumed in the reabsorption offiltered bicarbonate(B) Excreted in the urine as ammoniumions(C) Excreted in the urine as freehydrogen ions(D) Excreted in the urine as titratableacid

R E V I E W Q U E S T I O N S

(continued)

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CASE STUDY FOR CHAPTER 23Nephrotic Syndrome

A 6-year-old boy is brought to the pediatrician by hismother because of a puffy face and lethargy. A fewweeks before, he had an upper respiratory tract infec-tion, probably caused by a virus. Body temperature is36.8�C; blood pressure, 95/65; and heart rate, 90beats/min. Puffiness around the eyes, abdominalswelling, and pitting edema in the legs are observed. Aurine sample (dipstick) is negative for glucose but re-veals 3� protein. Microscopic examination of the urinereveals no cellular elements or casts. Plasma [Na�] is140 mEq/L; BUN, 10 mg/dL; [glucose], 100 mg/dL; creati-nine, 0.8 mg/dL; serum albumin, 2.3 g/dL (normal, 3.0 to4.5 g/dL); and cholesterol, 330 mg/dL. A 24-hour urinesample has a volume of 1.10 L and contains 10 mEq/LNa�, 60 mg/dL creatinine, and 0.8 g/dL protein.

The child is treated with the corticosteroid prednisone,and the edema and proteinuria disappear in 2 weeks.Puffiness and proteinuria recur 4 months later, and a re-nal biopsy is performed. Glomeruli are normal by light

microscopy, but effacement of podocyte foot processesand loss of filtration slits is seen with the electron micro-scope. No immune deposits or complement are seen af-ter immunostaining. The biopsy indicates minimalchange glomerulopathy. The podocyte cell surface andglomerular basement membrane show reduced stainingwith a cationic dye.

Questions

1. What features in this case would cause suspicion ofnephrotic syndrome?

2. What is the explanation for the proteinuria?3. Why does the abnormally high rate of urinary protein excre-

tion underestimate the rate of renal protein loss?4. What is the endogenous creatinine clearance, and is it nor-

mal? (The boy’s body surface area is 0.86 m2.)5. What is the explanation for the edema?

Answers to Case Study Questions for Chapter 23

1. The child has the classical feature of nephrotic syndrome:heavy proteinuria (8.8 g/day), hypoalbuminemia (3 g/dL),generalized edema, and hyperlipidemia (plasma cholesterol330 mg/dL).

CHAPTER 25 Acid-Base Balance 445

5. The following measurements weremade in a healthy adult:

Filtered bicarbonate 4,320 mEq/dayExcreted bicarbonate 2 mEq/dayUrinary titratable acid 30 mEq/dayUrinary ammonia 60 mEq/day

(NH4�)

Urine pH 5

Net acid excretion by the kidneys is(A) 28 mEq/day(B) 30 mEq/day(C) 88 mEq/day(D) 90 mEq/day(E) 92 mEq/day

6. If a patient with uncontrolled diabetesmellitus has a daily excretion rate of200 mEq of titratable acid and 500mEq of NH4

�, how many mEq of newHCO3

� have the kidney tubules addedto the blood?(A) 0 mEq(B) 200 mEq(C) 300 mEq(D) 500 mEq(E) 700 mEq

7. Which of the following causesincreased tubular secretion of hydrogenions?(A) A decrease in arterial PCO2

(B) Adrenal cortical insufficiency(C) Administration of a carbonicanhydrase inhibitor(D) An increase in intracellular pH(E) An increase in tubular sodiumreabsorption

8. A homeless woman was found on a hotsummer night lying on a park bench in

a comatose condition. An arterialblood sample revealed a pH of 7.10,PCO2 of 20 mm Hg, and plasma[HCO3

�] of 6 mEq/L. Plasma glucoseand blood urea nitrogen (BUN) valueswere normal. Plasma [Na�] was 140mEq/L and [Cl�] was 105 mEq/L.Which of the following might explainher condition?(A) Acute renal failure(B) Diarrhea as a result of foodpoisoning(C) Methanol intoxication(D) Overdose with a drug thatproduces respiratory depression(E) Uncontrolled diabetes mellitus

9. Which of the following arterial bloodvalues might be expected in amountain climber who has beenresiding at a high-altitude base campbelow the summit of Mt. Everest forone week?

PlasmaPo2 Pco2 [HCO32]

pH (mm Hg) (mm Hg) (mEq/L)(A) 7.18 95 25 9(B) 7.35 50 60 32(C) 7.53 40 20 16(D) 7.53 95 50 40(E) 7.62 40 20 20

10.A 25-year-old nurse is brought to theemergency department shortly beforemidnight. Although somewhat drowsy,she was able to relate that she hadattempted to kill herself by swallowingthe contents of a bottle of aspirintablets a few hours before. Which ofthe following set of arterial bloodvalues is expected?

PlasmaPo2 Pco2 [HCO32]

pH (mm Hg) (mm Hg) (mEq/L)(A) 7.25 95 19 8(B) 7.29 55 60 28(C) 7.40 95 40 24(D) 7.59 95 16 15(E) 7.70 95 16 19

SUGGESTED READING

Abelow B. Understanding Acid-Base. Balti-more: Williams & Wilkins, 1998.

Adrogue HJ, Madias NE. Management oflife-threatening acid base disorders. NEngl J Med 1998;338:26–34, 107–111.

Alpern RJ, Preisig PA. Renal acid-base trans-port. In: Schrier RW, Gottschalk CW,eds. Diseases of the Kidney. 6th Ed.Boston: Little, Brown, 1997;189–201.

Bevensee MO, Alper SL, Aronson PS,Boron WF. Control of intracellular pH.In: Seldin DW, Giebisch G, eds. TheKidney. Physiology and Pathophysiol-ogy. 3rd Ed. Philadelphia: LippincottWilliams & Wilkins, 2000;391–442.

Hood VL, Tannen RL. Protection of acid-base balance by pH regulation of acidproduction. N Engl J Med1998;339:819–826.

Knepper MA, Packer R, Good DW. Am-monium transport in the kidney. Phys-iol Rev 1989;69:179–249.

Lowenstein J. Acid and basics: A guide to un-derstanding acid-base disorders. NewYork: Oxford University Press, 1993.

Rose BD. Clinical Physiology of Acid-Baseand Electrolyte Disorders. 4th Ed. NewYork: McGraw-Hill, 1994.

Valtin H, Gennari FJ. Acid-Base Disorders.Basic Concepts and Clinical Manage-ment. Boston: Little, Brown, 1987.

CASE STUDIES FOR PART IV • • •

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446 PART VI RENAL PHYSIOLOGY AND BODY FLUIDS

2. Proteinuria is a consequence of an abnormally high perme-ability of the glomerular filtration barrier to the normalplasma proteins. This condition might be a result of an in-creased size of “holes” or pores in the basement membraneand filtration slit diaphragms. The decreased staining with acationic dye, however, suggests that there was a loss offixed negative charges from the filtration barrier. Recall thatserum albumin bears a net negative charge at physiologicalpH values, and that negative charges associated with theglomerular filtration barrier impede filtration of this plasmaprotein.

3. Proteins that have leaked across the glomerular filtrationbarrier are not only excreted in the urine but are reabsorbedby proximal tubules. The endocytosed proteins are digestedin lysosomes to amino acids, which are returned to the cir-culation. Both increased renal catabolism by tubule cellsand increased excretion of serum albumin in the urine con-tribute to the hypoalbuminemia. The liver, which synthe-sizes serum albumin, cannot keep up with the renal losses.

4. The endogenous creatinine (CR) clearance (an estimate ofGFR) equals (UCR � V)/PCR � (60 � 1.10)/0.8 � 82 L/day. Nor-malized to a standard body surface area of 1.73 m2, CCR is166 L/day �1.73 m2, which falls within the normal range(150 to 210 L/day �1.73 m2). Note that the permeability ofthe glomerular filtration barrier to macromolecules (plasmaproteins) was abnormally high, but permeability to fluidwas not increased. In some patients, a loss of filtration slitsmay be significant and may lead to a reduced fluid perme-ability and GFR.

5. The edema is a result of altered capillary Starling forces andrenal retention of salt and water. The decline in plasma[protein] lowers the plasma colloid osmotic pressure, favor-ing fluid movement out of the capillaries into the interstitialcompartment. The edema is particularly noticeable in thesoft skin around the eyes (periorbital edema). The abdomi-nal distension (in the absence of organ enlargement) sug-gests ascites (an abnormal accumulation of fluid in the ab-dominal cavity). The kidneys avidly conserve Na� (note thelow urine [Na�]) despite an expanded ECF volume. Al-though the exact reasons for renal Na� retention are contro-versial, a decrease in the effective arterial blood volumemay be an important stimulus (see Chapter 24). This leadsto activation of the renin-angiotensin-aldosterone systemand stimulation of the sympathetic nervous system, both ofwhich favor renal Na� conservation. In addition, distal seg-ments of the nephron reabsorb more Na� than usual be-cause of an intrinsic change in the kidneys.

Reference

Orth SR, Ritz E. The nephrotic syndrome. N Engl J Med1998;338:1202–1211

CASE STUDY FOR CHAPTER 24Water Intoxication

A 60-year-old woman with a long history of mental ill-ness was institutionalized after a violent argument withher son. She experiences visual and auditory hallucina-tions and, on one occasion, ran naked through the wardscreaming. She refuses to eat anything since admission,but maintains a good fluid intake. On the fifth hospitalday, she complains of a slight headache and nausea andhas three episodes of vomiting. Later in the day, she isfound on the floor in a semiconscious state, confusedand disoriented. She is pale and had cool extremities.Her pulse rate is 70/min and blood pressure is 150/100

mm Hg. She is transferred to a general hospital and, dur-ing transfer, has three grand mal seizures and arrives ina semiconscious, uncooperative state. A blood samplereveals a plasma [Na�] of 103 mEq/L. Urine osmolality is362 mOsm/kg H2O and urine [Na�] is 57 mEq/L. She isgiven an intravenous infusion of hypertonic saline (1.8%NaCl) and placed on water restriction. Several days aftershe had improved, bronchoscopy is performed.

Questions

1. What is the likely cause of the severe hyponatremia?2. How much of an increase in plasma [Na�] would an infu-

sion of 1 L of 1.8% NaCl (308 mEq Na�/L) produce? Assumethat her total body water is 25 L (50% of her body weight).Why is the total body water used as the volume of distribu-tion of Na�, even though the administered Na� is limited tothe ECF compartment?

3. Why is the brain so profoundly affected by hypoosmolality?Why should the hypertonic saline be administered slowly?

4. Why was the bronchoscopy performed?

Answers to Case Study Questions for Chapter 24

1. The problem started with ingestion of excessive amounts ofwater. Compulsive water drinking is a common problem inpsychotic patients. The increased water intake, combinedwith an impaired ability to dilute the urine (note the inap-propriately high urine osmolality), led to severe hypona-tremia and water intoxication.

2. Addition of 1 L of 308 mEq Na�/L to 25 L produces an in-crease in plasma [Na�] of 12 mEq/L. The total body water isused in this calculation because when hypertonic NaCl isadded to the ECF, it causes the movement of water out ofthe cell compartment, diluting the extracellular Na�.

3. Because the brain is enclosed in a nondistensible cranium,when water moves into brain cells and causes them toswell, intracranial pressure can rise to very high values.This can damage nervous tissue directly or indirectly by im-pairing cerebral blood flow. The neurological symptomsseen in this patient (headache, semiconsciousness, grandmal seizures) are consequences of brain swelling. The in-creased blood pressure and cool and pale skin may be aconsequence of sympathetic nervous system discharge re-sulting from increased intracranial pressure. Too rapidrestoration of a normal plasma [Na�] can produce seriousdamage to the brain (central pontine myelinolysis).

4. The physicians wanted to exclude the presence of a bron-chogenic tumor, which is the most common cause ofSIADH. No abnormality was detected. Today, a computedtomography (CT) scan would be performed first.

References

Grainger DN. Rapid development of hyponatremic seizures in apsychotic patient. Psychol Med 1992;22:513–517.Goldman MB, Luchins DJ, Robertson GL Mechanisms of al-tered water metabolism in psychotic patients with polydipsiaand hyponatremia. N Engl J Med 1988;318:397–403.

CASE STUDY FOR CHAPTER 25Lactic Acidosis and Hemorrhagic Shock

During a violent argument over money, a 30-year-oldman was stabbed in the stomach. The assailant escaped,but friends were able to rush the victim by car to thecounty hospital. The patient is unconscious, with a bloodpressure (mm Hg) of 55/35 and heart rate of 165beats/minute. Breathing is rapid and shallow. The sub-ject is pale, with cool, clammy skin. On admission, about

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an hour after the stabbing, an arterial blood sample istaken, and the following data were reported:

Patient Normal RangeGlucose 125 mg/dL 70–110 mg/dL (3.9–6.1 mmol/L)

(fasting values)Na� 140 mEq/L 136–145 mEq/LK� 4.8 mEq/L 3.5–5.0 mEq/LCl� 103 mEq/L 95–105 mEq/LHCO3

� 4 mEq/L 22–26 mEq/LBUN 23 mg/dL 7–18 mg/dL

(1.2–3.0 mmol/L urea nitrogen)Creatinine 1.1 mg/dL 0.6–1.2 mg/dL

(53–106 �mol/L)pH 7.08 7.35–7.45PaCO2 14 35–45 mm HgPaO2 97 mm Hg 75–105 mm HgHematocrit 35% 41–53%

Questions

1. What type of acid-base disturbance is present?2. What is the reason for the low PaCO2?3. Calculate the plasma anion gap and explain why it is high.4. Why is the hematocrit low?5. Discuss the status of kidney function.6. What is the most appropriate treatment for the acid-base

disturbance?

Answers to Case Study Questions for Chapter 25

1. The subject has a metabolic acidosis, with an abnormallylow arterial blood pH and plasma [HCO3

�].2. The low PaCO2 is a result of respiratory compensation. Ven-

tilation is stimulated by the low blood pH, sensed by the pe-ripheral chemoreceptors.

3. The anion gap is � [Na�] � [Cl�] � [HCO3�] � 140 � 103 �

4 � 33 mEq/L, which is abnormally high. Considering thehistory and physical findings, the high anion gap is mostlikely caused by inadequate tissue perfusion, with resultantanaerobic metabolism and production of lactic acid. The lac-tic acid is buffered by HCO3

� and lactate accumulates as theunmeasured anion. Note that tissue hypoxia can occur ifblood flow is diminished, even when arterial PO2 is normal.

4. The low hematocrit is a result of absorption of interstitialfluid by capillaries, consequent to the hemorrhage, low arte-rial blood pressure, and low capillary hydrostatic pressure.

5. In response to the blood loss and low blood pressure, kid-ney blood flow and GFR would be drastically reduced. Thesympathetic nervous system, combined with increasedplasma levels of AVP and angiotensin II, would produce in-tense renal vasoconstriction. The hydrostatic pressure in theglomeruli would be so low that practically no plasma wouldbe filtered and little urine (oliguria) or no urine (anuria)would be excreted. Because of the short duration of renalshutdown, plasma [creatinine] is still in the normal range;the elevated BUN is probably mainly a result of bleedinginto the gastrointestinal tract, digestion of blood proteins,and increased urea production.

6. Control of bleeding and administration of whole blood (orisotonic saline solutions and packed red blood cells) wouldhelp restore the circulation. With improved tissue perfusion,the lactate will be oxidized to HCO3

�.

CHAPTER 25 Acid-Base Balance 447