27 october 2017 kit components - lucigen.com kan_2_tnp_tra… · emergency number : 1-888-575-9695...

27 October 2017 Kit Components

Product Code Description

TSM99K2 EZ-Tn5™ <KAN-2>Tnp Transposome™ Kit

Components

KAN-2 FP-1 Forward Primer SS000523-D

KAN-2 RP-1 Reverse Primer SS000524-D

EZ-Tn5™ <KAN-2> TNP Transposon SS000588-D

Nuclease-Free Water, Sterile SS000772-D3

KAN-2 FP-1 Forward Primer. Safety Data Sheet Prepared according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations

Revision date: 10/31/2017 Version: A

10/31/2017 KAN-2 FP-1 Forward Primer Page 1

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1. Product identifier

Product name : KAN-2 FP-1 Forward Primer

Product form : Mixture

Product code : SS000523-D

1.2. Relevant identified uses of the substance or mixture and uses advised against

Use of the substance/mixture : Laboratory chemical.

1.3. Details of the supplier of the safety data sheet

Lucigen Corporation 2905 Parmenter Street Middleton, WI 53562 U.S.A. Phone: (608) 831-9011 Fax: (608) 831-9012 E-mail: [email protected]

1.4. Emergency telephone number

Emergency number : 1-888-575-9695 (Lucigen: Monday-Friday, 8:00AM-5:00PM)

SECTION 2: Hazards identification

2.1. Classification of the substance or mixture

GHS-US classification

Not classified.

2.2. Label elements

GHS-US labelling

No labelling applicable.

2.3. Other hazards

None.

2.4. Unknown acute toxicity (GHS-US)

No data available.

SECTION 3: Composition/information on ingredients

3.2. Mixture

Synonyms : N/A

No components need to be disclosed according to the applicable regulations.

SECTION 4: First aid measures

4.1. Description of first aid measures

First-aid measures general : If exposed or concerned, consult a physician. Show this safety data sheet to the doctor in attendance. Wash contaminated clothing before re-use. Never give anything to an unconscious person.

First-aid measures after inhalation : IF INHALED: Remove to fresh air and keep at rest in a comfortable position for breathing. If not breathing, give artificial respiration. Consult a physician.

First-aid measures after skin contact : IF ON SKIN (or clothing): Remove affected clothing and wash all exposed skin for at least 15 minutes with tepid water. Consult a physician.

First-aid measures after eye contact : IF IN EYES: Immediately flush with plenty of tepid water for at least 15 minutes. Remove contact lenses if present and easy to do so. Continue rinsing. Consult a physician.

First-aid measures after ingestion : IF SWALLOWED: Rinse mouth thoroughly and consult a physician. Do not induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cups of water. Wash mouth out with water. Consult a physician.

4.2. Most important symptoms and effects, both acute and delayed

Symptoms/injuries : Not expected to present a significant acute hazard under anticipated conditions of normal use.

Symptoms/injuries after inhalation : May cause upper respiratory irratation.

Symptoms/injuries after skin contact : May cause skin irritation.


10/31/2017 KAN-2 FP-1 Forward Primer 2/5

Symptoms/injuries after eye contact : Direct contact with the eyes is likely to be irritating.

Symptoms/injuries after ingestion : May cause gastrointestinal irritation.

4.3. Indication of any immediate medical attention and special treatment needed

No additional information available

SECTION 5: Firefighting measures

5.1. Extinguishing media

Suitable extinguishing media : Water spray, alcohol resistant foam, dry chemical, carbon dioxide, alcohol-resistant foam, or appropriate foam.

5.2. Special hazards arising from the substance or mixture

Fire hazard : No data available.

Explosion hazard : No data available.

Reactivity : No dangerous reactions known under normal conditions of use.

5.3. Advice for firefighters

Firefighting instructions : Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.

Protection during firefighting : Do not enter fire area without proper protective equipment, including respiratory protection.

SECTION 6: Accidental release measures

6.1. Personal precautions, protective equipment and emergency procedures

General measures : Ventilate area. Evacuate area. Keep upwind. Spill should be handled by trained clean-up crews properly equipped with respiratory equipment and full chemical protective gear (see Section 8).

6.1.1. For non-emergency personnel

Protective equipment : Wear Personal Protective Equipment as described in Section 8.

6.1.2. For emergency responders

Protective equipment : Wear suitable protective clothing, gloves, respirator, and eye or face protection. For further information refer to section 8: "Exposure controls/personal protection".

6.2. Environmental precautions

Prevent entry to sewers and public waters. Notify authorities if liquid enters sewers or public waters. Avoid release to the environment.

6.3. Methods and material for containment and cleaning up

For containment : Contain any spills with dikes or inert absorbents (e.g., sand or vermiculite) to prevent migration and entry into sewers or streams.

Methods for cleaning up : Soak up spills with inert absorbants, such as sand or vermiculite as soon as possible. Place in closed waste container for disposal. This material and its container must be disposed of in a safe way, and as per local, state, and federal legislation.

6.4. Reference to other sections


SECTION 7: Handling and storage

7.1. Precautions for safe handling

Precautions for safe handling : Do not handle until all safety precautions have been read and understood. Wear recommended personal protective equipment. Wash hands and other exposed areas with mild soap and water after handling material, leaving the laboratory, before eating, drinking or smoking and when leaving work.

7.2. Conditions for safe storage, including any incompatibilities

Storage conditions : Store at -20 °C. Keep container tightly closed.

SECTION 8: Exposure controls/personal protection

8.1. Control parameters

Contains no substances with occupational exposure limit values.

8.2. Exposure controls

Appropriate engineering controls : Provide adequate general and local exhaust ventilation. Use process enclosures, local exhaust ventilation, or other engineering controls to control airborne levels below recommended exposure limits. Ensure adequate ventilation, especially in confined areas. Emergency safety shower and eye wash station should be available. Avoid prolonged or repeated exposure.



Personal protective equipment : Gloves. Protective goggles. Laboratory Coat.

Hand protection : Use gloves chemically resistant to this material when prolonged or repeated contact could occur. Gloves should be classified under Standard EN 374 or ASTM F1296. Suitable gloves for this specific application can be recommended by the glove supplier. Suggested glove materials are: Neoprene, Nitrile.

Eye protection : Safety goggles should be worn when working with mixture. Avoid direct contact with eyes.

Skin and body protection : Wear long sleeves, and chemically impervious PPE/coveralls to minimize bodily exposure.

Respiratory protection : Use NIOSH/MSHA-approved dust/particulate respirator if irriration or other symptoms occur. Where vapor, mist, or dust exceed PELs or other applicable OELs, use NIOSH-approved respiratory protective equipment. Do not breathe in vapour, mist, or dust.

SECTION 9: Physical and chemical properties

9.1. Information on basic physical and chemical properties

Physical state : Liquid

Color : Clear

Odor : No data available

Odor Threshold : No data available

pH : No data available

Melting point : No data available

Freezing point : No data available

Boiling point : No data available

Flash point : No data available

Relative evaporation rate : No data available

Flammability (solid, gas) : No data available

Vapour pressure : No data available

Relative vapour density at 20 °C : No data available

Relative density : No data available

Solubility in Water : No data available

Log Pow : No data available

Log Kow : No data available

Auto-ignition temperature : No data available

Decomposition temperature : No data available

Viscosity, kinematic : No data available

Viscosity, dynamic : No data available

Explosive properties : No data available

Oxidising properties : No data available

Explosive limits : No data available

9.2. Other information

None.

SECTION 10: Stability and reactivity

10.1. Reactivity

No dangerous reactions known under normal conditions of use.

10.2. Chemical stability

Stable under use and storage conditions as recommended in section 7.

10.3. Possibility of hazardous reactions

None known. Hazardous polymerization does not occur.

10.4. Conditions to avoid

None known.

10.5. Incompatible materials

None known.

10.6. Hazardous decomposition products

Hazardous decomposition products may form under fire conditions. The nature of the decomposition products is not known.



SECTION 11: Toxicological information

11.1. Information on toxicological effects

Acute toxicity : No data available

Skin corrosion/irritation : No data available

Serious eye damage/irritation : No data available

Respiratory or skin sensitisation : No data available

Germ cell mutagenicity : No data available

Carcinogenicity : IARC – No component of this product present at levels greater than or equal to 0.1% is dientified as probablye, possible, or confirmed human carcinogen by IARC.

ACGIH – No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by ACGIH.

NTP – No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticpated carcinogen by NTP.

OSHA – No component of this product present at levels greater than or equal to 0.1% is identified as a carcinoen or potential carcinogen by OSHA.

Reproductive toxicity : No data available

Specific target organ toxicity (single exposure) : No data available

Specific target organ toxicity (repeated exposure)

: No data available

Aspiration hazard : No data available





Additional Information : None.

SECTION 12: Ecological information

12.1. Toxicity


12.2. Persistence and degradability


12.3. Bioaccumulative potential


12.4. Mobility in soil


12.5. Other adverse effects


SECTION 13: Disposal considerations

13.1. Waste treatment methods

Waste treatment methods : Obtain the consent of pollution control authorities before discharging to wastewater treatment plants. Product should not be discharged to surface waters without a NPDES permit.

Waste disposal recommendations : Dispose in a safe manner in accordance with local, state, and federal regulations. Avoid release to the environment.

SECTION 14: Transport information

In accordance with DOT

Not hazardous for transport

Additional information

Other information : No supplementary information available.

Transport by sea


Air transport




SECTION 15: Regulatory information

15.1. US Federal regulations

SARA 302 Components

No chemicals in this solution are subject to the reporting requirements of SARA Title III, Section 302.

SARA 304 Extremely Hazardous Substances Reportable Quantity

This product does not contain any components with a section 304 EHS RQ.

SARA 311/312 Hazards

No SARA Hazards

SARA 313

This materials does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.

15.2. International regulations None.

15.3. US State regulations

California Proposition 65

This product does not contain any substances known to the state of California to cause cancer and/or reproductive harm.

Massachusetts Right To Know Components No components are subject to the Massachusetts Right to Know Act. New Jersey Right to Know Hazardous Substance List Water, CAS 7732-18-5 Pennsylvania Right to Know List Water, CAS 7732-18-5

SECTION 16: Other information

Indication of changes : Revision A: Updated format.

Revision date : 10/31/2017

Other information : Author:

NFPA health hazard : 0 – Poses no health hazard, no precautions necessary and would offer no hazard beyond that of ordinary combustible materials.

NFPA fire hazard : 0 – Materials that will not burn under typical fire conditions, including intrinsically noncombustible materials such as concrete, stone, and sand.

NFPA reactivity : 0 - Normally stable, even under fire exposure conditions, and are not reactive with water.

HMIS III Rating

Health : 0

Flammability : 0

Physical Hazard : 0

Personal Protection :

This information is disclosed to the best of Lucigen’s knowledge. This document does not constitute a contractual relationship with product end users or handlers with respect to the possible presence of hazards in this item. Disposal should be in accordance with applicable regional, national and local laws and regulations.

0

0

0

KAN-2 RP-1 Reverse Primer. Safety Data Sheet Prepared according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations


10/31/2017 KAN-2 RP-1 Reverse Primer Page 1



Product name : KAN-2 RP-1 Reverse Primer












Not classified.

2.2. Label elements

GHS-US labelling

No labelling applicable.

2.3. Other hazards

None.


No data available.


3.2. Mixture

Synonyms : N/A

No components need to be disclosed according to the applicable regulations.







First-aid measures after ingestion : IF SWALLOWED: Rinse mouth thoroughly and consult a physician. Do not induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cups of water. Wash mouth out with water. Consult a physician.






10/31/2017 KAN-2 RP-1 Reverse Primer 2/5







Suitable extinguishing media : Water spray, alcohol resistant foam, dry chemical, carbon dioxide, alcohol-resistant foam, or appropriate foam.














Protective equipment : Wear suitable protective clothing, gloves, respirator, and eye or face protection. For further information refer to section 8: "Exposure controls/personal protection".










Precautions for safe handling : Do not handle until all safety precautions have been read and understood. Wear recommended personal protective equipment. Wash hands and other exposed areas with mild soap and water after handling material, leaving the laboratory, before eating, drinking or smoking and when leaving work.


Storage conditions : Store at -20 °C. Keep container tightly closed.











Skin and body protection : Wear long sleeves, and chemically impervious PPE/coveralls to minimize bodily exposure.

Respiratory protection : Use NIOSH/MSHA-approved dust/particulate respirator if irriration or other symptoms occur. Where vapor, mist, or dust exceed PELs or other applicable OELs, use NIOSH-approved respiratory protective equipment. Do not breathe in vapour, mist, or dust.




Color : Clear













Solubility in Water : No data available











None.


10.1. Reactivity





None known. Hazardous polymerization does not occur.


None known.


None known.


Hazardous decomposition products may form under fire conditions. The nature of the decomposition products is not known.

















: No data available






Additional Information : None.


12.1. Toxicity



















Transport by sea


Air transport






SARA 302 Components

No chemicals in this solution are subject to the reporting requirements of SARA Title III, Section 302.




No SARA Hazards

SARA 313

This materials does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.

15.2. International regulations None.








Other information : Author:




HMIS III Rating

Health : 0

Flammability : 0

Physical Hazard : 0



0

0

0

EZ-Tn5™ <KAN-2> TNP Transposon. Safety Data Sheet Prepared according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations


10/23/2017 EZ-Tn5™ <KAN-2> TNP Transposon Page 1



Product name : EZ-Tn5™ <KAN-2> TNP Transposon




Use of the substance/mixture : Laboratory Chemicals.








Not a hazardous substance or mixture.

2.2. Label elements

GHS-US labelling


2.3. Other hazards

No additional information available.


No data available.


3.1. Substance

Not applicable

3.2. Mixture

No ingredients are hazardous according to OSHA criteria. No components need to be disclosed according to the applicable regulations.



First-aid measures general : If exposed or concerned, get medical attention/advice. Show this safety data sheet to the doctor in attendance. Wash contaminated clothing before re-use. Never give anything to an unconscious person.


First-aid measures after skin contact : IF ON SKIN (or clothing): Remove affected clothing and wash all exposed skin with tepid water for at least 15 minutes. If symptoms continue, consult a physician.

First-aid measures after eye contact : IF IN EYES: Immediately flush with tepid water for at least 15 minutes. Remove contact lenses if present and can easy to do so. Continue rinsing. If symptoms continue, consult a physician.

First-aid measures after ingestion : IF SWALLOWED: Rinse mouth thoroughly. Do not induce vomiting. Consult a physician if symptoms persist. Do not give anything by mouth to an unconscious person.








10/23/2017 EZ-Tn5™ <KAN-2> TNP Transposon 2/5





Suitable extinguishing media : Incase of fire, use water, dry chemical, chemical foam, or alcohol-resistance foam. Use agents most appropriate to extinguish the fire.


Fire hazard : Product is not flammable.

Explosion hazard : Product is not explosive.



Firefighting instructions : Use water spray or fog for cooling exposed containers. Exercise caution when fighting any chemical fire. In the event of fire and/or explosion, do not breathe fumes. Do not dispose of fire-fighting water in the environment.






Protective equipment : Wear Protective equipment as described in Section 8.


Protective equipment : Wear suitable protective clothing, gloves and eye or face protection. For further information refer to section 8: "Exposure controls/personal protection".





Methods for cleaning up : Soak up spills with inert absorbants, such as vermiculite and sand. This material and its container must be disposed of in a safe way, and as per local, state, and federal legislation.





Precautions for safe handling : Do not handle until all safety precautions have been read and understood. Wear recommended personal protective equipment. Wash hands and other exposed areas with mild soap and water after working with mixture, before leaving the laboratory, before eating, drinking or smoking and when leaving work. Avoid ingestion and inhalation. Keep container tightly closed.


Storage conditions : Store in at either -20°C or -70°C . Keep container tightly closed.



Components with workplace control parameters



Appropriate engineering controls : Provide adequate general and local exhaust ventilation. Use process enclosures, local exhaust ventilation, or other engineering controls to control airborne levels below recommended exposure limits. Ensure adequate ventilation, especially in confined areas. Emergency safety shower and eye wash should be available.



Personal protective equipment : Gloves. Protective goggles.

Hand protection : Use gloves chemically resistant to this material when prolonged or repeated contact could occur. Gloves should be classified under Standard EN 374 or ASTM F1296. Suitable gloves for this specific application can be recommended by the glove supplier.

Eye protection : Use eye protection suitable to the environment. Avoid direct contact with eyes.

Skin and body protection : Wear long sleeves, and chemically impervious PPE to minimize bodily exposure.

Respiratory protection : Use NIOSH-approved dust/particulate respirator as needed. Where vapor, mist, or dust exceed PELs or other applicable OELs, use NIOSH-approved respiratory protective equipment.



Physical state : Liquid containing dissolved material

Color : Colorless



pH : ~ 7










Solubility : No data available













10.1. Reactivity





None known.


None known.


None known.


None known.





Acute toxicity : Not available

Skin corrosion/irritation : Not available

Serious eye damage/irritation : Not available

Respiratory or skin sensitisation : Not available

Germ cell mutagenicity : Not available

Carcinogenicity : Not available

Reproductive toxicity : Not available

Specific target organ toxicity (single exposure) : Not available


: Not available

Aspiration hazard : Not classified






12.1. Toxicity



















Transport by sea


Air transport




OSHA Hazards

No OSHA Hazards

CERCLA Reportable Quantity

This product does not contain any components with a CERCLA RQ.






No SARA Hazards

SARA 302

No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.

SARA 313

This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.

15.2. International regulations No additional information available.



This product does not contain any substances known to the state of California to cause cancer and/or reproductive harm

This mixture not listed on the following:

U.S. - Massachusetts - Right To Know List U.S. - New Jersey - Right to Know Hazardous Substance List U.S. - Pennsylvania - RTK (Right to Know) List


Indication of changes : Revision A: New SDS Created.


Other information : Author: Lucigen Corporation

NFPA health hazard : 0 - Exposure under fire conditions would offer no hazard beyond that of ordinary combustible materials.

NFPA fire hazard : 0 - Materials that will not burn.


HMIS III Rating

Health : 0

Flammability : 0

Physical : 0



Nuclease-Free Water, Sterile. Safety Data Sheet Prepared according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations


07/08/2017 Nuclease-Free Water, Sterile Page 1



Product name : Nuclease-Free Water, Sterile


Product code : SS000772-D3










2.2. Label elements


2.3. Other hazards

None.


No data available.


3.2. Mixture

Name Product identifier %

Water, CAS # 7732-18-5 EC# 231-791-2 Chemical Formula: H2O

Molecular Weight: 18.02 g/mol Synonyms: Sterile Water, RNase Freee Water

Ingredient in product. 100%

No ingredients are hazardous according to OSHA criteria. No components need to be disclosed according to the applicable regulations.







First-aid measures after ingestion : IF SWALLOWED: Never give anything by mouth to an unconscious person. Obtain medical assistance. Do NOT induce vomiting unless directed by medical personnel. If conscious and alter, rinse mouth and drink 2-4 cupfuls of water. Wash mouth out with water.




07/08/2017 Nuclease-Free Water, Sterile 2/5

Symptoms/injuries after inhalation : May cause irritation to respiratory tract.



Symptoms/injuries after ingestion : May cause irritation of the digestive tract.


No data available.



Suitable extinguishing media : Use extinguishing measures that are appropriate to the circumstances and environment.




Reactivity : Product does not burn.










Protective equipment : Wear suitable protective clothing, gloves, respirator, and eye or face protection. For further information refer to section 8: "Exposure controls/personal protection". Avoid contact with skin and eyes.










Precautions for safe handling : Do not handle until all safety precautions have been read and understood. Wear recommended personal protective equipment. Avoid breathing dust, vapour, mist, or gas. Avoid contact with eyes, skin, and clothing. Wash hands and other exposed areas with mild soap and water after handling material, leaving the laboratory, before eating, drinking or smoking and when leaving work.


Storage conditions : Store in a -20°C freezer without a defrost cycle.











Skin and body protection : Wear long sleeves, and chemically impervious PPE/coveralls to minimize bodily exposure as necessary.

Respiratory protection : Use NIOSH/MSHA-approved dust/particulate respirator if exposure symptoms develop. Where vapor, mist, or dust exceed PELs or other applicable OELs, use NIOSH-approved respiratory protective equipment. Do not breathe in vapour, mist, or dust.




Color : Clear




Melting point : 0°C

Freezing point : 0°C

Boiling point : 100°C





Relative vapour density at 20°C : No data available

Relative density : 1.000 g/cm3 at 3.98°C

Solubility in Water : Completely miscible











None.


10.1. Reactivity





None known.




None known.


None known.


None known.















: No data available


Symptoms/injuries after inhalation : May cause respiratory irratation.


Symptoms/injuries after eye contact : May cause eye irritation.


Additional Information : The chemical, physical, and toxicological properties have not been thoroughly investigated.


12.1. Toxicity















DOT

Not dangerous goods



IMDG

Not dangerous goods

IATA

Not dangerous goods



SARA 302

No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.




No SARA Hazards

SARA 313

This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.

15.2. International regulations.









Other information : Author: Lucigen Corporation




HMIS III Rating

Health : 0

Flammability : 0

Physical Hazard : 0



0

0

0

27 october 2017 kit components - lucigen.com kan_2_tnp_tra… · emergency number : 1-888-575-9695...

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