3 8

1 8

Archaea:Thermococcus kodakaraensis KOD1 DNA

Thermococcus kodakaraensis KOD1Growth temperature 60 100

optimum:85Obligatory anaerobic

Heterotrophic nutrition KOD DNA Polymerase

90.0kDa 93.9kDa

+

300 ND

TaqKOD

+

Species Thermococcuskodakaraensis KOD1

Thermusaquaticus

Molecular weight

95 , 12hr 95 , 1.6hrThermostability(half-life period)3'-5'exonuclease activity

Processivity (bases)

130 61Elongation rate(bases/sec)

Terminal transferase activity

50 1Fidelity

DNA

90.1kDa

+

20

PfuPyrococcusfuriosus

95 , 6hr

25

12

MasterMIX 2 8 1

-

CG

CG

A

G

CG

C

dna 1

NaLC 40min

MAC Primer

KOD mix 140 L 6mix (PPmix) 140 L 6

300 L 1300 L 1300 L 1

96

NaLC

GENECUBE

KODmix

PPmix

GENECUBE40min

3

1517

8

GENECUBE

360

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