3 d dfa simplex virus identification kit herpes simplex virus · 2015. 9. 16. · a complete system...
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A Complete System ApproachCombine the D3 DFA HSV Identification or Identification and Typing Kit with
H&V-Mix™* MixedCells™, Flocked Swabs and UTM for a complete system
approach to your Herpes Simplex Virus testing needs.
Rapid Turnaround TimeMost lesion or genital herpes cultures show positive results within 24 hours on
H&V-Mix MixedCells. Confirm CPE in as little as one 15 minute staining step.
Flexible Reagent FormatsOrder as a complete kit containing reagents, control slides, PBS and mounting
fluid, or order reagents independently as an Identification and Typing Duo Pack
(HSV-1 and HSV-2 Reagents packaged together) or Single HSV Identification
Reagent. It’s your choice!
Herpes Simplex Virus
identification within
24 hours using proven
Totally Tubeless™ MixedCells
technology from Diagnostic
HYBRIDS.
Herpes Simplex VirusIdentification & Typing Kits
D3® DFA
H&V-Mix™ cells infected with Herpes Simplex Virus Type 1, stained with D3 DFA Herpes Simplex Virus Identification Kit
H&V-Mix™ cells infected with Herpes Simplex Virus Type 2, stained with D3 DFA Herpes Simplex Virus Identification Kit
*US Patent No. 6,168,915 & 6,495,316
A Quidel Company For More Information, Contact Us www.dhiusa.com (866) 344-3477© 2010 Quidel Corporation. All rights reserved.
A Quidel Company For More Information, Contact Us www.dhiusa.com (866) 344-3477© 2010 Quidel Corporation. All rights reserved.
MKT0006v2010MAR26
H&V-Mix Product Codes
D3 DFA HSV KitsKits
01-080000 D3 DFA HSV Identification Kit
Includes: HSV Reagent (5-mL), 40X PBS Concentrate (25-
mL), Mounting Fluid (7-mL), HSV-1/HSV-2 Antigen Control
Slides (5 slides)
01-090000 D3 DFA HSV Identification & Typing Kit
Includes: HSV-1 Reagent (5-mL), HSV-2 Reagent (5-mL),
40X PBS Concentrate (25-mL), Mounting Fluid (7-mL), HSV-
1/HSV-2 Antigen Control Slides (5 slides)
Individual Components
01-090001 D3 DFA Herpes Simplex Virus
Identification & Typing Reagent
Duo Pack
Includes: HSV-1 DFA Reagent (5-mL), HSV-2 DFA
Reagent (5-mL)
01-085005 D3 DFA Herpes Simplex Virus
Reagent (5-mL)
01-00100 HSV-1/HSV-2 Antigen Control Slides
(10 slides per set)
01-090025 40X PBS Concentrate (25-mL)
01-002007b Mounting Fluid (7-mL)
Vials
98-0102 Shell Vials (with coverslip)
Plates
98-2412 24 well/12 fill
98-2418 24 well/18 fill
98-2424 24 well/24 fill
98-4812 48 well/12 fill
98-4818 48 well/18 fill
98-4824 48 well/24 fill
Standard 2% FBS Refeed Medium
10-320100 100-mL
10-320500 500-mL
Recommended Protocol
Clinical Specimens
HSV ID and Typing
Shell Vial 2Shell Vial 1
Inoculate 2 H&V monolayers with 200 to 400-µL of specimen
Stain with HSV-2 DFA Reagent for 15-30 min.
Stain with HSV DFA Reagent for 15-30 min
Examine for the presence of Fluorescent Stained Cells
Fluorescent Stained Cells present
Report “Herpes Simplex Virus Detected”
No Fluorescent Stained Cells present
Report “NO Herpes Simplex Virus Detected”
Stain with HSV-1 DFA Reagent for 15-30 min.
Centrifuge 700xg, 1-Hours, Incubate @ 35˚ - 37˚ Cfor 1 to 2 days
Examine for the presence of Fluorescent Stained Cells
Examine for the presence of Fluorescent Stained Cells
Fluorescent Stained Cells
present
Report “Herpes Simplex Virus
Type 1 Detected”
Report “NO Herpes Simplex Virus Detected”
Report “Herpes Simplex Virus
Type 2 Detected”
No Fluorescent Stained Cells
present
Fluorescent Stained Cells
present
HSV ID
Inoculate H&V monolayers with 200 to 400-µL of specimen
Centrifuge 700xg, 1-Hours, Incubate @ 35˚ - 37˚ Cfor 1 to 2 days
Hs27 ReadyCells® SystemHs27 ReadyCells
F-87-0102-24 24 Shell Vials - With Coverslip
ReadyCells Heat Block
99-610-009 ReadyCells Heat Block (120 volts)
(used for rapid, controlled thawing
of ReadyCells)
Herpes Simplex VirusIdentification & Typing Kits
D3® DFA