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There is generally recognized need for methods that permit rapid estimation of the

bacteriological quality of water. Applications of rapid method may range from

analysis of waste water to potable water quality assessment. Ideally, rapid

procedures are reliable and have sensitivity levels equal to those of the standard

tests routinely used. However, sensitivity of a rapid test may be compromised

because the bacterial limit sought may be below the minimum bacterial

concentration essential to rapid detection. Tests for detection of indicator

organisms, rather than pathogens are used generally in water testing. The

coliform group of bacteria is the principle indicator of suitability of water for

domestic, industrial or other uses.

Our Water Testing kits are designed to provide rapid, accurate, easy to use and

cost effective solutions to a wide variety of applications for water testing. Though

simple to use, they provide the latest in bacterial diagnosis technology to provide

consistent reliable results.

Water Testing SolutionsColiforms, E.coli and Enterococci are important indicators of faecal pollution in

water analysis. Some of the tests being dependent on enzyme substrate reactions,

these tests utilize hydrolysable substrates for detection of the above organisms.

When the enzyme technique is used, the total coliform group is defined as all

bacteria possessing the enzyme beta-D-galactosidase, which cleaves the

chromogenic substrate, resulting in release of the chromogen. E.coli are defined

as bacteria giving a positive total coliform response and possesseing the enzyme

beta-D-glucuronidase, which cleaves a fluorogenic substrate, resulting in the

release of the fluorogen. Enterococci possess the enzyme beta-D-glucosidase,

which cleaves the chromogenic substrate, resulting in release of the chromogen.

For procedures, storage, references, results and other information, refer

to the package insert of individual product.

Summary and Principle

The test includes detection of E.coli and coliforms based on enzyme substrate

reaction.

The coliform group of bacteria is the principle indicator of suitability of water for

domestic, industrial or other uses.

E.coli can be presumptively identified by the production of beta-

glucuronidase(GUD) which has been proposed as an alternative for IMViC tests.

The medium contains all nutrients required for the growth of coliforms and E. coli

Rapid Coliform Test Kitalong with the substrate. The non-fluorescent substrate is cleaved by the enzyme

GUD produced by E.coli to release the fluorogen (4-methylumbelliferone), which

exhibits a bluish fluorescence when exposed to long wave (366nm) ultraviolet

light. Coliforms, on the other hand, will not produce fluorescence.

Cultural Response

Organism (ATCC) Colour Fluorescence

E.aerogenes (13048) Blue green -

E.coli (25922) Blue green +


This kit is used for detection of coliforms in water from treatment plants or

distribution systems, the test being only presumptive.

The presence absence test for the coliform group is a simple modification of the

multiple tube procedure. Simplification, by use of one large test portion (100 ml)

in a single culture bottle to obtain qualitative information on the presence or

absence of coliforms, is justified on the theory that no coliforms should be present

in 100 ml of a drinking water sample

The medium contains nutrients required for the growth of coliforms. Lactose is the

PA Coliform Test Kitfermentable carbohydrate. Phosphates provide buffering action while sodium

lauryl sulphate inhibits organisms other than coliforms. Bromocresol purple is the

pH indicator which turns yellow at acidic pH.

Cultural Response

Organism (ATCC) Growth Colour

E.aerogenes (13048) Luxuriant Light yellow

E.coli (25922) Luxuriant Yellow

E.faecalis (29212) Inhibited -

K.pneumoniae (13883) Luxuriant Yellow

S.typhi (6539) Luxuriant No change (purple)


The test includes identification and differentiation of Enterococci from water

samples based on enzyme substrate reaction.

Rapid Enterococci Test KitThe presence of Enterococcus group, which is a subgroup of faecal streptococci,

serves as a valuable bacterial indicator for determining the extent of faecal

contamination and it is more specific than the detection of coliforms which may

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This kit is used for simultaneous detection of Salmonella, Vibrio species and E.coli

based on H S production.2

Hydrogen sulphide can be produced at least in small amounts from sulphur

containing amino acids. Some bacteria are capable of breaking down sulphur

containing amino acids (cystine, methionine) or reducing inorganic sulphur

containing compounds (sulfate, thiosulphate) to produce H S. This reduced 2

sulphur may then be incorporated into other cellular amino acids, or perhaps into

coenzymes. The medium incorporates sulphur containing compounds and iron

salts. The organisms reduce this sulphur containing compounds to sulphite and

H S gas by virtue of a reductive enzyme. Production of H S gas is detected as an 2 2

Rapid H S Kit2

insoluble black precipitate of ferrous sulphide formed upon reaction of H S with 2

iron salts. Formation of H S indicates presence of unwanted microorganisms 2

making the water unfit for drinking.

Cultural Response

Organism (ATCC) Growth H S production2

C.freundii (8090) + +

E.coli (25922) + -

P.mirabilis (25933) + +

P.vulgaris (13315) + +

S.typhi (6539) + +

V.cholerae (15748) + +

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Microbiological testing is both time-and labor intensive, which is why there was a

need to develop easier, faster methods for detecting microorganisms.

Our rapid methods for detection of microorganisms offer savings in both time and

labour. These are miniatured iidentification kits for detection of microorganisms

One Step Presumptive Identification Testsbased on various principles like enzyme production, ability to utilize a particular

substrate, detection of metabolic end products, etc.




The identification is based on fluorescence detection of E. coli using enzyme

substrate reaction.

E.coli are important indicators of faecal pollution. 92- 95% of E.coli possess b-

glucoronidase, which will hydrolyze MUG (4-methylumbelliferyl-b-D-

glucuronide) to yield 4-methylumbelliferone. This compound produces a visible

Rapid Presumptive Identification Kit For E.Coliblue green fluorescence when excited with long wave UV light(366nm).

Cultural Response

Organism (ATCC) Fluorescence

E.aerogenes (13048) -

E.coli (25922) +

originate from non-faecal sources. The medium contains all the essential

nutrients required for the growth of enterococci, and a suitable inhibitor to inhibit

accompanying bacterial flora, specially gram negative organisms. The enzyme

beta-D-glucosidase produced by enterococci cleaves the chromogenic substrate,

resulting in an intensive colour change in the broth from light yellow to blue green.

Cultural Response

Organism (ATCC) Growth Colour

E.coli (25922) Poor Yellow

E.faecalis (29212) Luxuriant Blue

green


The identification is based on PYR test. PYR is a rapid test to determine the ability

of the organism to produce the enzyme L-pyrrolidonyl peptidase (PYR). The test

can be used in differentiating streptococcus species. Following incubation with

PYR (L-pyrrolidonyl-b-naphthylamide) substrate, PYR positive streptococci will

Rapid PYR Test Kithydrolyze it to release L-pyrrolidone and b-naphthylamide. On addition of the

PYR Reagent, development of a cherry red colour indicates a positive test.

Cultural Response

Organism (ATCC) Result

S.agalactiae (27956) - (no change)

S.pyogenes (19615) + (red/pink)

397



The test includes presumptive identification of E.coli on the basis of enzyme

substrate reaction and Indole Test. Indole test alone, is not an adequate screen for

E.coli, since there are at least 52 species of gram negative bacilli that grow on

MacConkey media, that are indole positive and ferment lactose. These include

K.oxytoca, Citrobacter freundii, Serratia odorifera, and Aeromonas hydrophila.

The enzyme-substrate test therefore, in conjunction with Indole test can be used

as a rapid screening test to assist in the identification of E.coli.

Lactose fermentation is a classical identification test. It is normally demonstrated

by acid production after the dissacharide has been cleaved into galactose and

glucose by the enzyme beta-galactosidase.

The medium contains all essential nutrients required for growth of E.coli along

with a synthetic chromogenic substrate ortho-nitrophenyl-beta-D-

Rapid Test Kit For E.Coligalactopyranoside (ONPG), which is a substitute for lactose. ONPG is hydrolysed

in the same way as lactose, but being chromogenic produces a yellow colour.

Using this test system b-galactosidase positive , indole positive, gram-negative

organisms can be presumptively identified as E.coli. Hydrolysis of additive o-

nitrophenyl-b-D-galactopyranoside (ONPG) denotes presence of b-

galactosidase enzyme and is detected by development of yellow color in the

medium. Indole production is detected by addition of Kovac's Reagent.

Development of pink colour is a positive Indole test.

Cultural Response

Organism (ATCC) Result

ONPG Indole

E.aerogenes (13048) + (yellow) -(no change)

E.coli (25922) + (yellow) +(pink)


The test is based on detection of esculin hydrolysis and is used for the

identification of group D streptococci. Group D streptococci and enterococci are

able to split esculin.

The medium contains esculin, ferric citrate to provide ferric ions, and 1% oxbile to

inhibit most other strains of non-group D streptococci. Sodium azide is

incorporated into the medium to inhibit gram-negative bacteria. Esculin is

hydrolyzed by group D streptococci to form dextrose and esculetin. Esculetin reacts

with the ferric ions contained within the medium, turning the medium dark brown

Rapid Test Kit For Esculin Hydrolysisto black. Thus, the tolerance to the presence of bile and the hydrolysis of esculin

provide the means to presumptively identify group D streptococci. This test can

also be used for identification of Listeria, since all Listeria species hydrolyse

esculin.Cultural Response

Organism (ATCC) Result (esculin hydrolysis)

L.monocytogenes (19112) +(blackening of medium)

S.bovis (27960) +(blackening of medium)

S.pyogenes (19615) -

Rapid Test Kit For MR-VPSummary and Principle

The test includes detection of acid and acetoin production. Methyl red test detects

the production of sufficient acid during the fermentation of glucose and the

maintenance of conditions such that the pH of an old culture is sustained below

4.4 as shown by a change in colour of the Methyl Red Indicator. Methyl red

positive organisms produce high levels of acid during fermentation of dextrose,

overcome the phosphate buffer system and produce a red color upon addition of

Methyl Red Indicator.

Voges Proskauer test detects the production of acetylmethylcarbinol (acetoin), a

natural product formed from pyruvic acid in the course of glucose fermentation.

The red color produced by the addition of Barritt Reagent A and Barritt Reagent B

tot he cultures is due to the ability of organism to produce a neutral product

acetoin. The acetoin is oxidized in the presence of oxygen and alkali to produce

diacetyl, which reacts in the presence of creatine to produce a red color.

Cultural Response

Organisms (ATCC) Result

MR VP

E.aerogenes (13048) -(yellow) +(red)

E.coli (25922) +red) -(no change)

K.pneumoneae (23357) -(yellow) +(red)




The test is based on the visualization of phenylalanine deamination reaction and

is used to differentiate Proteus and Providencia from enteric bacilli

Members in the genera Proteeae of Enterobacteriaceae have the ability to

deaminate phenylalanine to phenylpyruvic acid by enzymatic activity.

Phenylalanine serves as a substrate for the enzyme, which is able to deaminate it

to form phenylpyruvic acid. The addition of TDA Reagent following incubation

Rapid Test Kit For Phenylalanine Deaminationresults in appearance of deep green color in case of positive reaction or no color

change in case of negative reaction.Cultural Response

Organism (ATCC) Result/Colour


P.alcalifaciens (9886) +(green)

P.vulgaris (13315) +(green)

Ready Prepared Media

Ready prepared media are both time and labor saving. We offer a variety of ready

media (100 ml) in glass bottles intended for use in food and dairy industry, water

and waste water treatment plants, cosmetics, pharmaceuticals, medicine, etc.

For relevant information like summary, medium formulation, quality

control and results, references, refer to the individual product in

dehydrated media section. For directions of use and storage conditions

refer to the bottle label of individual product.

Alkaline Peptone water is used as an enrichment medium for the cultivation of

vibrio species from clinical and nonclinical specimen. It is also useful for

preliminarily enrichment of vibrios from faeces or other contaminated materials.

Direction

1. Use as per requirements

A highly nutritious general purpose liquid medium for cultivation of variety of

fastidious and non-fastidious microorganisms, including aerobic and anaerobic

bacteria.

Direction

1. Use as per requirements.

Blood Agar Base is a non-selective general-purpose medium to which blood may

be added for use in isolation and cultivation of streptococci, and other fastidious

pathogenic organisms like Neisseria, etc. It is also used for detection of

haemolytic activity.

Directions

1. Loosen the cap.02. Melt the medium in water bath at 100 C.

03. For preparation of blood agar, cool the base to 45-50 C and aseptically add

5% v/v sterile, defibrinated blood.

ALKALINE PEPTONE WATER

BHI BROTH

BLOOD AGAR BASE

4. Mix well and pour plates.

Brain Heart Infusion Agar is a general-purpose medium used for the cultivation of

a wide variety of organisms including bacteria, yeasts and moulds.

Directions


3. Mix well to distribute the precipitate uniformly and pour plates.

C.L.E.D. Agar with Andrade indicator is used for the isolation, enumeration and

presumptive identification of microorganisms from urine, giving good colonial

differentiation.

Directions


3. Mix well and pour plates.

A basal medium for preparation of blood and chocolate agar and for various

selective and identification media in isolating and cultivating fastidious

microorganisms.

Direction

1. Loosen the cap.02. Melt the medium in water bath at 100 C. Avoid overheating.

03. Cool to 45 C and pour plates.

EMB Agar, Levine is a slightly selective and differential medium used for the

isolation, enumeration and differentiation of members of Enterobacteriaceae.

BRAIN HEART INFUSION AGAR

CLED AGAR WITH ANDRADE INDICATOR

COLUMBIA BLOOD AGAR BASE

EMB AGAR, LEVINE

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Directions


03. Cool to 45 C and shake the medium in order to restore the blue colour

(oxidize the methylene blue) and to suspend the precipitate which is an

essential part of the medium

4. Pour plates.

Glucose Broth used for the study of cultivation and fermentation studies of

microorganisms and for testing the sensitivity of microorganisms to antibiotics by

the tube dilution method.

Direction


Glucose Phosphate Broth is used for differentiation of coli-aerogene group by

means of Methyl Red and Voges Proskauer reactions.

Direction


A medium for cultivation of wide variety of bacteria from blood specially

fastidious streptococci and Corynebacterium diptheriae.

Direction


MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts is a slightly

selective and differential medium for the detection of coliforms and enteric

pathogens.

Directions



MacConkey Agar without Crystal Violet, NaCl & with Sodium Tauracholate is used

for the cultivation and differentiation of enteric bacteria and potentially

pathogenic gram-positive organisms, while restricting swarming of Proteus

species.

Directions



GLUCOSE BROTH

GLUCOSE PHOSPHATE BROTH

HARTLEY BROTH

MAcCONKEY AGAR WITH CV, NaCl and 0.15% BILE SALTS

MAcCONKEY AGAR W/O CV, NaCl and W/ SODIUM TAUROCHOLATE

MUELLER HINTON AGAR

NUTRIENT AGAR

NUTRIENT BROTH

PEPTONE WATER

PLATE COUNT AGAR

PHOSPHATE BUFFER USP

Mueller Hinton Agar is used for antimicrobial disc diffusion susceptibility testing

of common, rapidly growing bacteria by the Bauer-Kirby method.

Directions


03. Cool to 45 C, mix well and pour plates.

Nutrient Agar is used as a general-purpose culture medium for the cultivation of

bacteria, which may also be used as enrichment media by incorporating 10% v/v

sterile blood or other biological fluids.

Directions



Nutrient Broth are used as general-purpose culture media for the cultivation of

microorganisms.

Direction


Use

Peptone Water and Peptone Water with Phenol Red are non-selective media used

for cultivating non-fastidious organisms and as a base for carbohydrate

fermentation media.

Directions



Use

Plate Count Agar (Standard Methods Agar) is used for obtaining microbial plate

counts from milk and dairy products, foods, water and other materials of sanitary

importance.

Directions



Phosphate Buffer USP is used as diluent for raw material and finished product

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SIMMONS CITRATE AGAR

SOYABEAN CASEIN DIGEST AGAR

SS AGAR

TSB + 25% CAP4

TRIPLE SUGAR IRON AGAR

VIOLET RED BILE AGAR

Simmons Citrate Agar is used for the differentiation of gram-negative bacteria on

the basis of citrate utilization.

Directions


03. Cool to 45 C and pour tubes.

4. Keep in slanted position for use as slants.

Soyabean Casein Digest Agar is a general-purpose medium used for the isolation

and cultivation of a wide variety of fastidious and non-fastidious microorganisms.

Directions

1. Loosen the cap.02. Melt the medium in water bath at 100 C. Do not overheat.


A differential and selective medium for isolation of Salmonella and some Shigella

species from clinical and non-clinical specimens.

Direction



A medium for detection and isolation of bacterial contamination from clean

surface and accidentally contaminated raw material samples of pharmaceutical

and cosmetic formulations.

Triple Sugar Iron Agar is used for the identification of gram-negative enteric

bacilli on the basis of dextrose, lactose and sucrose fermentation and hydrogen

sulphide production.

Directions


03. Cool to 45 C and pour tubes.

5. Allow the medium to set in sloped form with a butt about 1 inch long.

Violet Red Bile Agar is a selective medium used for the detection and enumeration

of coliforms.

Directions

1. Loosen the cap.

testing as per US pharmacopoeia.

Plate Count Agar (Standard Methods Agar) is used for obtaining microbial plate

counts from milk and dairy products, foods, water and other materials of sanitary

importance.

Directions



Potato Dextrose Agar is used for the cultivation and enumeration of yeasts and

moulds from dairy and other food products.

Directions


3. When required to alter the reaction of the medium to pH 3.5, cool the base to 045 C and aseptically add an appropriate amount of sterile 10% tartaric acid

(approximately 1ml in 100 ml of medium) and mix well.04. Cool to 45 C and pour plates.

5. Do not reheat the medium after addition of acid.

A medium recommended for selective enrichment of Salmonella species under

condition of high osmotic pressure and low pH with modest nutritional

requirement.

Direction


Sabouraud Dextrose Agar is a general-purpose medium used for the cultivation of

yeasts, moulds and aciduric bacteria.

Directions



Saline is used as a diluent for raw material and finished product testing.

An enrichment medium for the isolation of Salmonella species from faeces, urine,

water, foods and other material of sanitary importance.

Direction


PLATE COUNT AGAR

POTATO DEXTROSE AGAR

RAPPAPORT VASSILIADIS SALMONELLA ENRICHMENT BROTH USP

SABOURAUD DEXTROSE AGAR

SALINE

SELENITE BROTH

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Blood Culture System is a fast, efficient and simpler detection system for

preliminary identification of microorganisms from blood.

The indications for obtaining blood cultures are manifold but are basically the

occurrence of a sudden relative change in pulse rate and temperature, with or

without chills, prostration, and hypotension; a history of mild, intermittent, and

persistent fever in association with a heart murmur; and generally any time sepsis

is suspected.

The technology of blood culture is revived by Gould and Duerden (1983). In most

bacterial infections of the blood in man the organisms are not numerous, and it is

essential for their demonstration by blood culture that a relatively large amount of

blood, e.g. 5-10 ml, should be used as the inoculum. In children and infants the

order of magnitude of bacteremia is considerably greater, so that a small amount

of blood (usually 1-5 ml) can be drawn. As the blood's natural bactericidal or

bacteriostatic action may interfere with the growth of any bacteria present, this

effect should be annulled by diluting the blood with medium. There is evidence

that upto 10 ml of blood, or probably more, may be added to 100 ml of medium

without a detectable antibacterial effect. For practical reasons, it is usual to add

5-7 ml of blood to about 50 ml of medium. An anticoagulant is added to the

medium to avoid clotting of blood, which would entrap bacteria and prevent their

detection. The most commonly used preparation in blood culture media today is

0.025% to 0.05% Sodium Polyanethol Sulfonate (SPS) (liquoid). In addition to

its anticoagulant properties, SPS is also anticomplementary, antiphagocytic, and

interferes with the activity of some antimicrobial agents, notably

aminoglycosides.

Blood culture includes qualitative detection of microorganisms in a patient's

blood. Culture of a patient's blood is one of the most important investigations in

clinical microbiology, for the demonstration of septicaemia or bacteriemia

indicates that there is an immediate threat to the patient's life and an urgent need

for appropriate antibiotic therapy.

Blood culture is requested mainly in two clinical situations: 1) where the

possibility of septicaemia or bacteriaemia is suggested by the presence of fever,

shock or other symptoms occurring in association with a known or suspected local

infection such as sepsis in a surgical wound, puerperal sepsis, pneumonia,


02. Melt the medium in water bath at 100 C.03. Cool to 45 C and pour into plates containing the inoculum.

XLD Agar is a moderately selective medium used for the isolation and

differentiation of Salmonella and Shigella.

XLD AGAR

Directions

1. Loosen the cap.02. Melt the medium in water bath at 100 C. Do not overheat.


Blood Culturing Systems

meningitis, osteomyletis or endocarditis; and 2) where it is one of the procedures

required in the investigation of a fever difficult to diagnose because of the absence

of signs of a specific infection or local infective lesion, i.e. a pyrexia of unknown

origin.

The isolation of a bacterium from the blood of a patient from a local infection such

as wound sepsis or pneumonia is valuable firstly in indicating the urgent need for

antibacterial therapy, secondly in revealing the species of bacterium against

which therapy should be directed, and thirdly in providing a culture for the

performance of in-vitro drug sensitivity tests.CULTURE MEDIUM

It is advisable to seed more than one medium for blood culture. The wide range of

media includes brain heart infusion broth, cooked meat medium, fastidious

anaerobe broth, thioglycollate broth and soyabean casein digest broth.

The chance of detecting scanty or exacting bacteria will be maximized if a variety

of each media are seeded from each sample and different media are used for

aerobes and anaerobes.

CONTAMINATION

A difficulty in interpreting results arises from the liability of the specimen to be

contaminated with organisms that are commensals or common contaminants on

the skin and the finding of such organisms in the blood culture should be viewed

with the strong suspicion that it is a contaminant from the skin. But in a patient

with debility, immune deficiency, an intravascular line, a prosthetic implant or

some other condition favouring an opportunistic infection, the saprophytic or

commensal organism isolated may indeed have an important pathogenic role.

Consideration must be given to the probability of it having such an opportunistic

role in the circumstances of each particular case.

COLLECTION OF SAMPLE

To minimize difficulty in interpretation, every care must be taken to prevent

contamination of the specimen during its collection from the patient and its

examination in the laboratory. Proper procedures must be adopted for collection.

False negative results: The media and methods used for blood culture should be

such as to support growth of nutritionally exacting organisms, and both strict

aerobes and anaerobes as well as facultative organisms. Even so, a single


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negative culture should not be taken as proof that a patient does not have a blood

infection. In bacteriaemic illnesses the number of bacteria in blood may vary

rapidly between many and few or none as they are introduced intermittently from

a local infective focus and removed by the reticuloendothelial tissues. To

maximize the chances of isolation when the bacteria may be scanty, a large

volume of blood, e.g. 10-20 ml, from each collection should be cultured, and a

number of samples should be collected on separate occasions.

ANTIBIOTICS IN THE BLOOD

The blood sample should be diluted in the culture medium in order to reduce the

concentration of natural antibacterial constituents to a sub-effective level. The

dilution also reduces the concentration of any therapeutically administered

antibiotic. For this reason, every attempt should be made to ensure that samples

of blood are collected before the start of antibiotic therapy. If an antibiotic has

already been administered, the collection of the blood sample should preferably

be delayed until at least 24 hours after a dose has last been given. Otherwise, it

may be possible to add an antibiotic-inactivating agent to the culture bottle.

CULTURE SYSTEMS

Decisions have to be made about 1) the kinds and numbers of culture media to be

seeded from each sample of bottle, 2) the methods of incubation of the culture

bottles in the laboratory, 3) the number and times of examinations of the bottles

during their incubation, and the methods of examination by filming and

subculture.

CULTURE BOTTLES

It is necessary to culture at least several milliliters of blood diluted 1 in 5 to 1 in 10

in a culture medium. To maximize the chances of detecting scanty bacteria, a

larger volume, e.g. 10-20 ml of blood should be collected and half of it should be

inoculated into each of a 'set' of two such culture bottles. The use of two bottles has

several further advantages: they may contain different media or may be

incubated in different atmospheres, and scanty contaminants are likely to grow in

only one of them. The blood is inoculated into the bottle by inserting the syringe

needle through the rubber washer, the bottle remaining unopened and

unexposed to the entry of airborne dust.EXAMINATION OF BLOOD CULTURE

Depending on the number of bacteria in the blood stream and other factors, the

first appearance of growth may take place after as little as a few hours or only

after a longer period of 1 or a few days, or occasionally one or two weeks. As it is

desired to detect any positive culture as early as possible and yet not miss any late

growing culture, the ideal procedure is to make repeated examinations of the

culture bottle at different times during its incubation from 4 hours to two weeks or

longer.

Trials of different schedules suggest that a minimal, reasonably effective

procedure is the making of just two examinations on each bottle, the first after 0incubation at 37 C overnight, the second after 4-7 days. Only very few cultures

negative at 4 days become positive on longer incubation. When a report is required

urgently on a suspected septicaemia, an additional examination may be made

after only 4 hours incubation.

Some workers, however, consider that an examination should always be made on

the day the specimen is received, i.e. after incubation for only 4-8 hours. Positive

findings at that stage are available for earlier reporting to the clinician and are less

likely to be due to the growth of scanty contaminants than those obtained after

longer incubation. Also, the negative cultures, have to be examined again at 18-

24 hours and 4-7 days, requiring a total of three examinations.

SUBCULTURES

It is recommended that on each subsequent occasion of examination, the blood

culture should be examined both by a gram film and by subculturing on two plates

of blood agar, the one incubated in air, plus 5-10% CO , the other anaerobically in 2

nitrogen/hydrogen plus 5-10% CO . If the presence of meningococcus or 2

haemophilus seems likely, e.g. in suspected septicaemic meningitis, a heated

blood agar (chocolate agar) plate may be substituted for the aerobic plain blood

agar plate. Gram filming is particularly valuable when the first examination is

made only after overnight incubation, i.e. after 12-24 hours, for when bacteria are

seen to be present in the film, a preliminary report may be given to the physician at

once.

We offer blood culture media bottles to meet the wide range of blood culture

demands that are put on hospitals and reference labs. These help to improve

patient diagnosis and treatments and reduce health care costs by optimizing

septicemia diagnosis and maximizing lab efficiency, safety and productivity. These

support the recovery of bacteria and fungi present in blood.

For procedures, storage, directions for use, references, results and other

information, refer to the package insert of individual product.

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BHI With 0.05% SPSSoyabean Casein Digest Broth With 0.05% SPS


The occurrence of a sudden relative change in pulse rate and temperature with or

without chills, hyperventilation are indications of suspected septicemia.

Septicemia in hospital patients has increased largely, with a corresponding

increase in morbidity and mortality. Hence, for cases of suspected septicemia, the

culturing of blood for determination of bacteria and fungi becomes mandatory.

Blood culture bottles are used primarily for culturing blood to detect aerobic,

facultative, or anaerobic microorganisms present in the blood stream. The

medium contains all essential nutrients to allow the recovery of microorganisms

present. Blood is withdrawn from the patient using a sterile needle and added to

the blood culturing bottle containing 0.05% Sodium Polyanethol Sulfonate

(SPS). SPS is a polyanionic anticoagulant which inhibits complement and

lysozyme activity, interferes with phagocytosis and inactivates antibiotics,

particularly aminoglycosides. After appropriate incubation, the bottle is observed

for turbidity, colour change, hemolysis, gas formation and other evidence of

growth.

Reagent

BHI WITH 0.05% SPS

INGREDIENTS GRAMS/LITRE

Calf brain Infusion 9.0

Beef Infusion 9.0

Proteose Peptone 10.0

Dextrose 2.0

Sodium Chloride 5.0

Di-Sodium Phosphate 2.0

SPS 0.5

pH: 7.4±0.2

SOYABEAN CASEIN DIGEST BROTH WITH 0.05% SPS


Tryptone 15.0

Soytone 5.0

Sodium Chloride 5.0

Dipotassium Phosphate 2.5

Dextrose 2.5

SPS 0.5

pH:7.3 ±0.2

GLUCOSE BROTH – SUPPLEMENTED WITH 0.05% SPS


Glucose 5.0

Tryptone 10.0

Sodium Chloride 5.0

SPS 0.5

pH: 7.3 + 0.2

Interpretation of Results

The medium allows good recovery of microorganisms present which can be further

identified by Gram stain and subculturing onto appropriate medium. Growth in

the broth medium is indicated by the presence of turbidity.

Mycobacteriology

The genus Mycobacterium are 'acid fast bacilli', which are difficult to stain, but

once stained resist decolourization with acid and alcohol. They include the

organisms of human tuberculosis, M. tuberculosis, bovine tuberculosis, M.bovis,

and avium tuberculosis, M.avium, and that of leprosy, M.leprae, which is not fully

acid- and alcohol-fast. The commonest species are classified in two groups: 1) the

typical tubercle bacilli, M.tuberculosis and M.bovis, and their variants, and 2)

the atypical mycobacteria, comprising most members of the genus mycobacteria

including commensal, saprophytic and opportunistically pathogenic species

(photochromogens, scotochromogens, non-chromogens, rapid growers). They

are commonly referred to as tuberculoid bacilli or mycobacteria other than

tubercle (MOTT).

Of particular importance are the tubercle bacilli, mainly M.tuberculosis, being

responsible for over 90% of all cases of tuberculosis, is almost exclusively a

parasite of humans and has mainly an airborne mode of transmission. Infection

with Mycobacterium tuberculosis remains a major public health problem.

Although effective methods for its control are available and have been applied

successfully for several decades, in some regions the prevalence of tuberculosis is

still inordinately high and remains the leading cause of death among notifiable

infectious diseases.

Atypical mycobacteria (tuberculoid bacilli or MOTT) mainly being commensals

and saprophytes may cause opportunistic infections in man indistinguishable

clinically, radiologically and histologically from that caused by the human

tubercle bacillus. Such infection is called mycobacteriosis, is not notifiable as

tuberculosis and generally there is no person-to-person transmission.

Culture, isolation and sensitivity of M.tuberculosis from patient groups using

standard methods remain the gold standard for its detection and effective

Ready to use...

Glucose Broth – Supplemented With 0.05% Sps


Microxpress

treatment worldwide. Differentiation of M.tuberculosis is possible on the basis of

rate and temperature of growth, cultural characteristics and morphology. Also,

various biochemical and other tests have been used to differentiate tubercle

bacilli from mycobacteria other than tubercle (MOTT) and also to differentiate

between M.tuberculosis and M.bovis (niacin and catalase reactions, nitrate

activity, pigment production, TCH and PNB sensitivity, drug susceptibility

patterns, etc). The identification of mycobacteria to the species level is important

because of its clinical significance, as also in order to provide adequate disease

management because specific antimycobacterial drugs are required against

different pathogenic species of mycobacteria.We offer a range of products for mycobacteriology as listed below. For procedures,

storage, references, results and other information, refer to the package insert of

individual product.

Ready to use...


Mycocult is a ready to use Lowenstein-Jensen solid medium for M. tuberculosis

isolation

Lowenstein-Jensen medium supports good growth of M.tuberculosis. It contains

all essential nutrients required for the cultivation of M.tuberculosis. Also, glycerol

present in the formulation enhances the growth of M.tuberculosis. Malachite

green present in optimum concentration not only inhibits contaminating

microflora, but also provides the desired colour contrast for easy identification of

M.tuberculosis colonies.

MycocultReagent

Mycocult LJ medium is provided as a ready to use slant. It is a standard non-

selective inspissated egg based solid medium for the isolation of M.tuberculosis

from specimens such as sputum, CSF and urine.


M. tuberculosis colonies may be detected from third week onwards upto eight

weeks. The colonies are characterized by granular buff coloured growth, which

have an initial size of 1-3 mm and full grown size of 5-8 mm.

Mycocult PYSummary and Principle

Mycocult PY is a ready to use solid medium for the isolation of M.bovis. L.J.

medium contains all essential nutrients required for the cultivation of M.bovis.

Also, sodium pyruvate present in the formulation enhances the growth of M.bovis.

Malachite green present in optimum concentration not only inhibits

contaminating microflora, but also provides the desired colour contrast for easy

identification of M.bovis colonies.

Reagent

Lowenstein-Jensen medium is provided as a ready to use slant. It is a standard

non-selective inspissated egg based solid medium containing sodium pyruvate

for the isolation of M.bovis from specimens such as sputum, CSF and urine.


M.bovis colonies can be detected from third week onwards up to eight weeks. The

colonies are characterized by tiny transparent growth which later becomes white.


The genus Mycobacterium are acid-fast bacilli, which are difficult to stain, but

once stained resist decolourization with acid and alcohol. Mycostain is an acid fast

stain for screening of M.tuberculosis and M.leprae.

Mycostain is a standard Ziehl Neelsen hot stain set for the screening of

M.tuberculosis from specimens such as sputum, CSF, urine and isolated culture. It

is also used in the identification of M.leprae from ulcerated nodules on skin and

ear lobe, scrapings and secretions from nasal mucosa and sputum specimens.

Carbol fuchsin forms acid insoluble complex with the mycolic acid present in the

cell walls of the acid- fast bacilli and renders red/pinkish red colour to

Mycostain

M.tuberculosis or M.leprae. M.tuberculosis being acid fast in nature, resists

decolourization by strong acid. Other elements present in the smear are

decolourized with sulphuric acid and hence take up the counter stain (methylene

blue) and are stained blue.Reagent

Mycostain comprises of

a) Mycostain A-Ready to use carbol fuchsin.

b) Mycostain B-Ready to use Loeffler's methylene blue.


a) Acid-fast bacilli stain pink to red colour.

b) Pus cells and other contaminants stain purple to blue colour.


ga

lle

ry

“If you want to be a winner you have to be willing to give it your all.”

Dehydrated Culture Media ■ Bases ■ Supplements

Bacteroides Bile Esculin Agar Baird Parker Agar

Uninoculated Plate

Bismuth Sulphite Agar

Uninoculated Plate

Bordet Gengou Agar Base

Candida albicansATCC 10231

Brain Heart Infusion Agar

Uninoculated Plate

Brilliant Green Agar Modified

Salmonella typhiATCC 19430

Bordetella pertussisATCC 8467

Bordetella parapertussisATCC 15311

Salmonella typhimuriumATCC 14028

Bacteroides fragilisATCC 25285

Uninoculated Plate Staphylococcus aureusATCC 25923


AccumixPicture Gallery...


Brilliant Green Bile Agar Cetrimide Agar Base

Columbia Blood Agar Base Columbia Blood Agar Base

Uninoculated Plate

Dexoycholate Citrate Agar

Uninoculated Plate

Dextrose Tryptone Agar

Staphylococcus aureusATCC 25923

Bacillus coagulansATCC 7050

Uninoculated Plate Uninoculated Plate Pseudomonas aeruginosaATCC 27853

Enterobacter aerogenesATCC 13048

Escherichia coliATCC 25922

Salmonella enteritidisATCC 13076


Streptococcus pyogenesATCC 19615

StreptococcuspneumoniaeATCC 6305





EMB Agar, Levine Lactobacillus MRS Agar


MacConkey Agar

Uninoculated Plate

MacConkey Agar w/o CV

Uninoculated Plate

Malt Agar

Uninoculated Plate

Mannitol Salt Agar

Proteus mirabilisATCC 12453


Staphylococcus epidermidisATCC 12228


Lactobacillus delbrueckii subsp. lactisATCC 7830

Aspergillus nigerATCC 16404


Saccharomyces cerevisiaeATCC 9763


Proteus mirabilisATCC 12453




Potato Dextrose Agar Pseudomonas Isolation Agar

Sabouraud Dextrose Agar Soyabean Casein Digest Agar

Shigella flexneriATCC 12022

SS Agar

Vibrio choleraeATCC 14033

TCBS Agar


Uninoculated Plate Pseudomonas aeruginosaATCC 27853

Aspergillus nigerATCC 16404




Trichophyton mentajrophytesATCC 9533


Streptococcus pneumoniaeATCC 6305





Tomato Juice Agar Violet Red Bile Agar

Uninoculated Plate

Vogel & Johnson Agar

Uninoculated Plate

WL Nutrient Agar


XLD Agar

Yersinia enterocoliticaATCC 9610

Yersinia Selective Agar



Uninoculated Plate Uninoculated Plate Enterobacter aerogenesATCC 13048

Lactobacillus rhamnosusATCC 9595


Staphylococcus aureusATCC 25923with Potassium Tellurite





Brain Heart Infusion Broth Brilliant Green Bile Broth 2%

Kligler Iron Agar Lactose Broth

Simmons Citrate Agar Triple Sugar Iron Agar

UninoculatedTube


Uninoculated Tube


Uninoculated Tube



Neisseria meningitidisATCC 13090



Enterobacter aerogenesATCC 13048

Uninoculated Tube



UninoculatedTube


Uninoculated Tube



In many cases scientists and health professionals need to identify a microbe in a

given environment. Learning the members of a population of microbes present in

nature can give us new insights into biochemical processes that are taking place.

This leads to better understanding of environments and the role microbes play in

them. Identifying a microbe growing in a patient will help identify the disease

caused and thereby the treatments that are effective at eliminating it.

Identification tests can also be used to monitor and eliminate microorganisms

present in the food supply, thereby increasing food safety by eliminating

pathogens and decreasing spoilage.

Bacteria of different genera can differ greatly in their morphological

characteristics. These differences can help us in identifying different bacteria.

Likewise, some bacterial species differ in their cellular morphologies and staining

properties. Again, these differences can be used to aid in identifying bacterial

species. Generally selective and/or differential media are used to aid in

identifying bacterial species. However, many bacteria may share the same

morphological and cultural characteristics-responses to differential staining, size

and shape of the individual cells, size and shape of the colonies, the growth rate in

the presence or absence of oxygen, at different temperatures, in varied pH ranges,

and many others-so these features are rarely sufficient to classify an organism.

Various strains, species and genera of organisms exhibit characteristic patterns of

substrate utilization, metabolic product formation and sugar fermentation. To

determine the identification of a bacterium, it is necessary to study its

metabolism, characterizing the distinctive biochemical reactions that take place

within a cell. The type of biochemical reaction each organism undergoes act as a

“thumbprint” for its identification. Metabolic reactions involve enzymes (bacteria

are among the most diverse organisms with respect to the type of enzyme they

contain), which are proteins that catalyze biochemical reactions and therefore

identification and classification of bacteria is based on the distinctive enzymatic

Microorganism Identification

makeup of bacterial cells.

Carbohydrate utilization studies is an offshoot technology related to biochemical

analysis. All organisms require a carbon source, as carbon is the backbone

material of biological molecules. Most bacteria obtain energy from the oxidation

of organic compounds such as sugars. Strict aerobes oxidize sugars to carbon

dioxide and water through the process of aerobic oxidation while strict anaerobes

perform fermentation reactions. Facultative anaerobes posess the enzymes that

allow them to perform either fermentation or respiration depending on the

availability of oxygen. The types of compounds that an organism can ferment and

the end products that are produced depends on the enzymes the microorganism

can make. Among the end products commonly generated by fermentation are

organic acids and gases such as carbon dioxide and hydrogen. A positive

fermentation reaction is indicated by a change in the colour of the indicator

present in the medium.

When identifying a suspected organism, a series of differential media containing

indicators are inoculated and incubated. One can determine if a given bacterial

species can utilize a given carbohydrate by checking for the presence of

byproducts that are produced by the oxidation of these carbohydrates. pH

indicators present in the media detect metabolic acids that have been produced

by the bacteria after the oxidation and fermentation of sugars giving some form of

visible reaction such as a colour change. Alternatively, reagents may be added to

media after the bacteria have grown; these reagents react with specific byproducts

or intermediates in a metabolic pathway to give a colour change. The results of

these tests on the suspected organism are then compared to known standards to

confirm its identification.

We offer a series of biochemical test kits, which are standardized miniaturized

versions of conventional procedures (biochemical tests).

General Biochemical Identification TestsSUMMARY AND PRINCIPLE

Biochemical tests used for identification of bacteria are most often qualitative.

Media used in these types of test are usually general type media containing

indicators, suitable for the growth of most organisms.

The tests are based on the principle of pH change and substrate utilization

designed to identify various metabolic properties of different bacterial species

that can be used to differentiate even closely related organisms. The kit allows

one to quickly perform approximately 12 tests on a sample. The kits contain

sterile broth for carbohydrate utilization, substrate utilization and other tests for

identification of organisms. The broths are reconstituted by adding a bacterial

suspension. After incubating for an appropriate period, the broths are examined

when the various indicator systems present are affected by the metabolites or

added reagents to give a colour change.

Package insert supplied in the kit allows one to understand the procedure, type of

components that are present and the way they aid in the identification of bacteria.

The system also contains an identification index (easy to read chart), result

interpretation chart and data entry chart that allows one to quickly identify an

unknown isolate. For procedures, storage, references, interpretation of

results, complete list of organisms that can be identified with individual

kit and precautions refer provided package insert of respective kit.


Microxpress

Candida Identification Kit is a standardized identification system, comprising of

12 miniatured biochemical tests for identification of Candida species. The test

allows identification of the isolate in 24 hours.

Candida Identification Test KitThe kit contains sterile broth for Urease detection and 11 different carbohydrates

for utilization study-Melibiose, Lactose, Maltose, Sucrose, Raffinose, Galactose,

Trehalose, Cellobiose, Inositol, Xylose, Dulcitol.

E.coli Identification Kit is a standardized identification system, comprising of 12

miniatured biochemical tests for identification of E.coli.

Being one of the most important members of the Enterobacteriaceae, E.coli are

gram negative, lactose fermenting organisms and are the commonest cause of

uncomplicated infections of lower urinary tract. They are routinely isolated from

food, feces, water and other clinical samples. The kit contains sterile broth for

E. Coli Identification Test KitMethyl Red test, Voges Proskauer test, Citrate utilization, Indole test,

Glucuronidase, Nitrate reduction, ONPG, Lysine utilization and 4 different

carbohydrates-Glucose, Lactose, Sucrose, Sorbitol.

Reagents supplied with the kit include Barritt Reagent A, Barritt Reagent B and

Creatine for VP test, Methyl Red Indicator for MR test, Nitrite detection agents

(Nitrite Detection Strip and Zinc Dust ) and Kovac's Reagent for Indole test.

Enterobacteriaceae Identification kit is a standardized identification system,

comprising of 12 miniatured biochemical tests for identification of

Enterobacteriaceae.

Basically gram-negative rods, they are the most frequently isolated bacteria from

clinical specimens. The kit can be used for screening pathogenic organisms from

enteric, urine and other relevant specimens.

Enterobacteriaceae Identification Test KitThe kit contains sterile broth for Indole test, Methyl Red test, Voges Proskauer

test, Citrate utilization, and 8 different carbohydrates for utilization study-

Glucose, Adonitol, Arabinose, Lactose, Sorbitol, Mannitol, Rhamnose, Sucrose).

Reagents supplied with the kit include Kovac's Reagent for Indole test, Methyl Red

Indicator for MR test, Barritt Reagent A, Barritt Reagent B, and Creatine for VP

test.

Gram Negative Identification Kit is a standardized identification system,

comprising of 13 miniatured biochemical tests for identification of gram negative

rods.

Gram Negative identification kit can be used for screening of suspected gram-

negative species isolated from feces, urine, blood or any other similar clinical

isolates. The kit can also be used for validation of known strains. The test allows

identification of the isolate in 6-8 hrs, i.e. in a day's working hours.

Gram-Negative Identification Test KitThe kit contains sterile broth for Lysine utilization, Ornithine utilization, Urease

detection, Phenylalanine deamination (TDA), Nitrate reduction, H S production, 2

Citrate utilization oxidase detectionand 5 different carbohydrates for utilization

study-Glucose, Adonitol, Arabinose, Lactose, Sorbitol.

Reagents supplied with the kit include TDA Reagent and Nitrite detection agents

(Nitrite Detection Strip and Zinc Dust).

Listeria Identification Kit is a standardized identification system, comprising of 12

miniatured biochemical tests for identification of Listeria species.

The kit is used for screening food and clinical samples and also for validating

known strains.

The kit contains sterile broth for Esculin hydrolysis, Voges Proskauer test, Nitrate

Listeria Identification Test Kitreduction, Methyl Red test, Catalase and 7 different carbohydrates-Glucose,

Xylose, Lactose, Mannitol, Rhamnose, alpha-Methyl-D-Mannoside, Ribose.

Reagents supplied with the kit include Barritt Reagent A, Barritt Reagent B, and

Creatine for VP test, Methyl Red indicator and Nitrite detection agents (Nitrite

Detection Strip and Zinc Dust).

Ready to use...


Microxpress

Neisseria Identification Kit is a standardized identification system, comprising of

12 miniatured biochemical tests for identification of Neisseria.

Basically gram-negative rods, they inhabit the mucous membranes of humans.

Neisseria Identification Kit can be used for screening pathogenic Neisseria from

nasopharyngeal, urethral, cervical, rectal and pharyngeal specimens.

The kit contains sterile broth for Urease detection, Voges Proskauer test, Oxidase,

Neisseria Identification Test KitCatalase, Nitrate reduction, ONPG, and 6 different carbohydrates-Glucose,

Maltose, Lactose, Sucrose, Fructose, Mannose.


Creatine for VP test, Gordon McLeod Reagent (Oxidase Reagent) and Nitrite

detection agents (Nitrite Detection Strip and Zinc Dust).

Salmonella Identification Kit is a standardized identification system, comprising

of 12 miniatured biochemical tests for identification of salmonellae.

Salmonella identification kit can be used for screening of feaces, urine, blood or

any other similar clinical specimens suspected of containing Salmonella.

The kit contains sterile broth for Methyl Red test, Voges Proskauer test, Citrate

Salmonella Identification Test Kitutilization, Urease detection, H S production, ONPG, Lysine utilization and 5 2

different carbohydrates-Arabinose, Lactose, Maltose, Sorbitol, Dulcitol).


Creatine for VP test and Methyl Red Indicator for MR test.

Staph Identification Kit is a standardized identification system, comprising of 12

miniatured biochemical tests for identification of Staphylococcus.

Staphylococci are gram positive, non motile organisms of major medical

significance. The kit can be used to screen various clinical specimens.

The kit contains sterile broth for Voges Proskauer test, Phosphatase, ONPG,

Staph Identification Test KitArginine utilization, Urease detection, and 7 different carbohydrates for

utilization study- Arabinose, Lactose, Mannitol, Sucrose, Raffinose, Trehalose,

Maltose.


Creatine for VP test.

Strep Identification Kit is a standardized identification system, comprising of 12

miniatured biochemical tests for identification of streptococci.

The kit contains sterile broth for Esculin hydrolysis, Voges Proskauer's test,

Arginine utilization, PYR hydrolysis, ONPG and 7 different carbohydrates for

Strep Identification Test Kitutilization study-Glucose, Arabinose, Sorbitol, Mannitol, Sucrose, Raffinose,

Ribose.


Creatine for VP test and PYR Reagent.

Ready to use...


No. Test Reagent to be added Principle Original Colour Positive Negativeof Medium Reaction Reaction

BIOCHEMICAL TESTS RESULT INTERPRETATION CHART

1 Indole test 2-3 drops of Kovac's Reagent Detects deamination of tryptophan Colourless Pinkish-Red Colourless

2 Methyl Red test 1-2 drops of Methyl Red Indicator Detects acid production Colourless Red Yellow

3 Voges Proskauer test 1-2 drops of Creatine, 2-3 drops of Detects acetoin production Colourless Pinkish-Red Colourless/Baritt Reagent A, and 1-2 drops of slight copperBaritt Reagent B

4 Citrate utilization - Detects capability of organism to utilize citrate as sole Yellow-green Blue Yellow greencarbon source

5 Glucose utilization - Detects glucose utilization Red Yellow Red /Pink

6 Adonitol utilization - Detects adonitol utilization Red Yellow Red /Pink

7 Arabinose utilization - Detects arabinose utilization Red Yellow Red /Pink

8 Lactose utilization - Detects lactose utilization Red Yellow Red /Pink

9 Sorbitol utilization - Detects sorbitol utilization Red Yellow Red /Pink

10 Mannitol utilization - Detects mannitol utilization Red Yellow Red /Pink

11 Rhamnose utilization - Detects rhamnose utilization Red Yellow Red /Pink

12 Sucrose utilization - Detects sucrose utilization Red Yellow Red /Pink

13 Raffinose utilization - Detects raffinose utilization Red Yellow Red /Pink

14 Trehalose utilization - Detects trehalose utilization Red Yellow Red /Pink

15 Maltose utilization - Detects maltose utilization Red Yellow Red /Pink

16 Ribose utilization - Detects ribose utilization Red Yellow Red /Pink

17 Mellibiose utilization - Detects mellibiose utilization Red Yellow Red /Pink

18 Galactose utilization - Detects galactose utilization Red Yellow Red /Pink

19 Cellulose utilization - Detects cellulose utilization Red Yellow Red /Pink

20 Inositol utilization - Detects inositol utilization Red Yellow Red /Pink

Ready to use... Microxpress


No. Test Reagent to be added Principle Original Colour Positive Negativeof Medium Reaction Reaction

21 Xylose utilization - Detects xylose utilization Red Yellow Red /Pink

22 Dulcitol utilization - Detects dulcitol utilization Red Yellow Red /Pink

23 Fructose utilization - Detects fructose utilization Red Yellow Red /Pink

24 Mannose utilization - Detects mannose utilization Red Yellow Red /Pink

25 Alpha-methyl-D-mannoside - Detects alpha-methyl-D-mannoside utilization Red Yellow Red /Pinkutilization

26 Lysine decarboxylase - Detects lysine decarboxylation Olive green Purple

27 Ornithine decarboxylase - Detects ornithine decarboxylation Olive green Purple

28 Phenylalanine deamination 2-3 drops of TDA Reagent Detects phenylalanine deamination activity Colourless Green Colourless

29 Arginine utilization Detects arginine utilization Olive green Purple

30 Oxidase detection 1-2 drops of Gordon McLeod Detects oxidase activity Colourless Purplish Colourless(Oxidase) Reagent

31 Catalase detection 3% H O soln. Detects catalase activity Effervescence No 2 2

seen effervescence

32 Urease detection - Detects urease activity Orangish yellow Pink Orangish yellow

33 Nitrate reduction Nitrite Detection Strip and a pinch Detects nitrate reduction Colourless Pink/red/violet Colourlessof Zinc Dust

34 H S production - Detects H S production Orangish Black Orangish2 2

35 ONPG utilization - Detects beta- galactosidase activity Colourless Yellow Colourless

36 Glucuronidase detection - Detects glucuronidase activity Colourless Blue green No fluoresance

37 Alkaline phosphatase 1-2 drops of 40% NaoH Detects phosphatase enzyme Cream to Pink Cream

38 Esculin hydrolysis - Detects esculin hydrolysis Cream-amber Black Cream

39 PYR test 1-2 drops of PYR Reagent Detects pyroglutamate aminopeptidase activity Cream Cherry red Cream

BIOCHEMICAL TESTS RESULT INTERPRETATION CHART

Olive - GreenYellow

Olive - GreenYellow

Olive - GreenYellow

fluoresance



IDENTIFICATION INDEX FOR SOME IMPORTANT ORGANISMS

MR

TE

ST

VP

TE

ST

CIT

RA

TE U

TILI

ZATI

ON

IND

OLE

TE

ST

LYS

INE

UTI

LIZA

TIO

N

OR

NIT

HIN

E D

ECA

RD

OXY

LATI

ON

AR

GIN

INE

UTI

LIZA

TIO

N

UR

EA

SE

DE

TEC

TIO

N

TDA

TE

ST

NIT

RA

TE R

ED

UC

TIO

N

CA

TALA

SE

DE

TEC

TIO

N

OX

IDA

SE

DE

TEC

TIO

N

HS

PR

OD

UC

TIO

N2

GLU

CO

SE

UTI

LIZA

TIO

N

AD

ON

ITO

L U

TILI

ZATI

ON

LAC

TOS

E U

TILI

ZATI

ON

AR

AB

INO

SE

UTI

LIZA

TIO

N

SO

RB

ITO

L U

TILI

ZATI

ON

ME

LIB

IOS

E U

TILI

ZATI

ON

MA

LTO

SE

UTI

LIZA

TIO

N

SU

CR

OS

E U

TILI

ZATI

ON

GA

LAC

TOS

E U

TILI

ZATI

ON

CE

LLO

BIO

SE

UTI

LIZA

TIO

N

INO

SIT

OL

UTI

LIZA

TIO

N

XY

LOS

E U

TILI

ZATI

ON

DU

LCIT

OL

UTI

LIZA

TIO

N

RA

FFIN

OS

E U

TILI

ZATI

ON

TRE

HA

LOS

E U

TILI

ZATI

ON

RA

MN

OS

E U

TILI

ZATI

ON

MA

NN

OS

E U

TILI

ZATI

ON

GLU

CU

RO

NID

AS

E D

ETE

CTI

ON

ALK

ALI

NE

PH

OS

PH

ATA

SE

ON

PG

UTI

LIZA

TIO

N

PY

R H

YD

RO

LYS

IS

ES

CU

LIN

HY

DR

OLY

SIS

ALP

HA

M-D

-MA

N

RIB

OS

E U

TILI

ZATI

ON

FRU

CTO

SE

UTI

LIZA

TIO

N

ORGANISMS

KEY: Based on % strains showing reactions following symbols have been assigned from laboratory results and standard references

+ = Positive (more than 90%) - = Negative (more than 90%) v = 11-98% variable result ND = No data W =Weak reaction

Aeromonas hydrophila d d - - - + + - + - d + -

Candida albicans - - - + - + - - + - - +

Candida tropicalis - - - + + v + - + - - v

Citrobacter freundii + - (+) d - (-) d - + - (+) + - d + + (+) + +

Enterobacter aerogenes - + + - + + - - + - - + + + + + + + +

Enterobacter cloacae - + + - - + d - + - + (-) + + + + + +

Escherichia coli + - - + + d - - + - - + - + + + d + +

Escherichia blattae + - d - + + - - + - + - -

Klebsiella pneumoniae subspecies - + + - + - + - + - - + + + + + + + +pneumonia

Listeria monocytogenes + + - + + v - + - + + -

Morganella morganii + - - + - + + + + - + - - - - - - -

Neisseria gonorrhoea - - + + + - - - - - -

Neisseria meningitidis - - + + + - + - - - -

Proteus mirabilis + d d - - + + + + + + - - - - + - -

Proteus vulgaris + - (-) + - - + + + - + + - - - - + - -

+ - - - -

ND

NDND



Pseudomonas aeruginosa - + + ND - -

Salmonella cholerasuis subspecies + - + - + + - - + - + + - - + + - v + + -cholerasuis

Salmonella enteritidis + - + - + + - - + - + + - - + +

Salmonella serotype typhi + - - - + - - - + - + + - - - + v

Salmonella serotype typhimurium + - + - + - + - + + + v -

Serratia marcesans (-) + + - + + (-) - + - + d - - + + - +

Shigella boydii, Shigella flexneri, Shigella + - - d - - - - + - + - - d d - - +dysenteriae

Shigella sonnei + - - - - + - - + - + - - + - - v +

Staph. aureus + w w + - + + - + + + -

Staph. epidermidis + w + v - + + - - - + -

Strep. pyogenes - + ND ND - ND - - ND + v

Vibrio cholerae ND + + - - - + + - ND - - -

Vibrio vulnificus + + v - v + + - + - v - -

Yersinia enterocolitica + - - d - + (+) - + - + - - + + + - +

ND ND ND ND + ND ND

IDENTIFICATION INDEX FOR SOME IMPORTANT ORGANISMS

MR

TE

ST

VP

TE

ST

CIT

RA

TE U

TILI

ZATI

ON

IND

OLE

TE

ST

LYS

INE

UTI

LIZA

TIO

N

OR

NIT

HIN

E D

ECA

RD

OXY

LATI

ON

AR

GIN

INE

UTI

LIZA

TIO

N

UR

EA

SE

DE

TEC

TIO

N

TDA

TE

ST

NIT

RA

TE R

ED

UC

TIO

N

CA

TALA

SE

DE

TEC

TIO

N

OX

IDA

SE

DE

TEC

TIO

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ORGANISMS

KEY: Based on % strains showing reactions following symbols have been assigned from laboratory results and standard references

+ = Positive (more than 90%) - = Negative (more than 90%) v = 11-98% variable result ND = No data W =Weak reaction



Microxpress

As seen, various strains, species, and genera of microorganisms exhibit

characteristic patterns of substrate utilization, metabolic product formation and

sugar fermentation. Often, biochemical tests are used to identify microorganisms.

The result can be seen by a colour change. Most reactions are based on the

detection of an enzyme with a certain given substrate. Also, methods to detect

certain metabolites by chemical reaction and or complex building techniques are

used. At the end a colour change gives a result which leads to more cognition of

the unknown organism.

Analytical reagents are used for carrying out biochemical tests used to identify

microorganisms.

In general, different bacteria convert dextrose and glucose to pyruvate using

different metabolic pathways. Some of these pathways produce unstable acidic

Readymade Analytical Reagentsproducts which quickly convert to neutral compounds. Some organisms use the

butylene glycol pathway, which produces neutral end products including acetoin

and/or 2,3-butanediol. Other organisms use the mixed acid pathway, which

produces stable acidic end products such as lactic, formic and acetic acid. A

medium like MR-VP Broth is used to determine the pathway a given organism

uses to ferment glucose; to this end reagents like Methyl Red Indicator or Barritt

Reagent A and B are added along with Creatine to detect end products or

intermediates produced by a given fermentation pathway.

We offer microbiologists carefully standardized analytical reagents in dropper

bottles in order to ensure accurate and interpretable results.




Baritt Reagent A, Barritt Reagent B and Creatine are used to perform the Voges

Proskauer (VP) test.

The VP test detects organisms that utilize the butylene glycol pathway. These

organisms ferment carbohydrates with the production of acetoin (acetyl methyl

carbinol) or its reduction product 2,3-butylene glycol. The substances can be

tested for by a colorimetric reaction between diacetyl (formed during the test by

oxidation of acetoin or 2,3-butylene glycol) and a guanidino group under

alkaline conditions. When Barritt Reagent A (alpha naphtol) and Barritt Reagent

B (KOH) are added to MR-VP Broth along with Creatine (catalyst) that has been

Barritt Reagent A, Barritt Reagent B, Creatineinoculated with an organism that uses butylene glycol pathway, development of

red colour is a positive reaction for formation of a neutral end product (acetoin).

Reagent

Baritt Reagent A comprises of 40% potassium hydroxide.

Baritt Reagent B comprises of 5% alpha naphtol in absolute alcohol.

Creatine comprises of 0.5% Creatine.

Cultural Response

Organism (ATCC) VP test

E.coli (25922) -

K.pneumoneae (23357) +


Methyl Red Indicator is used to perform the Methyl Red (MR) test.

The MR test involves adding the pH indicator methyl red to an inoculated tube of

MRVP Broth. If the organism uses the mixed acid fermentation pathway and

produces stable acidic end products, the acids will overcome the buffers in the

medium and produce an acidic environment. When Methyl Red Indicator is

added, if acidic end products are present, the Methyl Red Indicator will remain red

which is a positive test for stable acidic end products.

Methyl Red IndicatorReagent

Methyl Red Indicator comprises of methyl red 0.02%.

Cultural Response

Organism (ATCC) MR test


E.coli (25922) +

K.pneumoneae (23357) -

The MR and VP tests are generally employed in conjunction to detect specific

breakdown products and are more often used for identification of enteric bacteria.


Gordon McLeod Reagent is used for detection of oxidase activity of a bacterial

culture, i.e. to see if the organism is an aerobe.

Gordon McLeod Reagent (Oxidase Reagent)The test depends on the presence in bacteria of certain oxidases that will catalyze

the transport of electrons between electron donors in bacteria and a dye-N,N,N',N

tetramethyl-p-phenylenediamine dihydrochloride (Gordon McLeod Reagent ).

Ready to use...


Microxpress

Oxidases are enzymes that catalyze the reduction of oxygen during respiration.

For example in gram positive bacteria and many gram negative bacteria

cytochrome oxidase performs the final step in electron transport, reducing oxygen

to water. Other bacteria, such as the Enterobacteriaceae, do not reduce oxygen

using this enzyme. The test is a check for the presence of electron transport chain

that is the final phase of aerobic respiration. Normally, oxygen is the final electron

acceptor in this system. In this test, an artificial electron acceptor (Gordon McLeod

Reagent) is used in place of oxygen. This acceptor is a chemical that changes

colour to dark blue/purple when it takes the electron from the last element

(cytochrome oxidase) in the electron transport chain. (the cytochrome c oxidase

present in most gram negative bacteria will oxidize N,N,N',N-tetramethyl-p-

phenylenediamine dihydrochloride to give a dark purple complex).

The test is used for screening species of Neisseria, Aeromonas, Vibrio,

Campylobacter and Pseudomonas, which give positive reactions and for

excluding the Enterobacteriaceae, all species of which give negative reactions.

Reagent

Gordon McLeod Reagent comprises of 0.5% N,N,N',N-tetramethyl-p-

phenylenediamine dihydrochloride.

Cultural Response

Organism (ATCC) O x i d a s e

test

C.jejuni (29428) +

N.gonorrheae (19424) +


E.coli (25922) -


The ability to degrade amino acids to identifiable end products is often used to

differentiate bacteria. Production of indole using pure cultures in tryptophan-

containing media is recommended as an aid in differentiation of bacteria. Kovac's

Reagent is used for performing the Indole test. The test determines the ability of

certain bacteria, primarily Enterobacteriaceae to decompose the amino acid

tryptophan to indole which accumulates in the medium. Indole is then tested for

by a colorimetric reaction with Kovac's Reagent (p-dimethyl-amino-

benzaldehyde) Indole reacts with the aldehyde present in the Kovac's Reagent to

give a reddish-pink colour in the alcohol layer which is a positive test and is

particularly useful in the identification of E.coli. The alcohol layer extracts and

concentrates the red coloured complex.

Reagent

Kovac's Reagent comprises of 5% p-dimethyl-amino-benzaldehyde.

Cultural Response

Organism (ATCC) Indole test


E.coli (25922) +

Ready to use...

McFarland Standard No. 0.5

McFarland Standard are used as a reference to adjust the turbidity of bacterial

suspensions and in antimicrobial susceptibility testing.

McFarland Standard is turbidity standard incorporating a suspension of barium

sulphate ions or latex particles (particle size is equal to bacterial cell size ) in

buffer, which makes it turbid. Turbidity of bacterial suspension depend on the

number of bacteria present in suspension. Measurement of turbidity

quantifies the amount of bacteria present in bacterial suspension. If we require a

bacterial suspension of specific number of bacteria, we need to match opacity or

turbidity of bacterial suspension with a chosen opacity standard like McFarland.

Turbidity of 0.5 McFarland standard is equal to a suspension, which contains

1.5X10 numbers of bacteria.

McFarland Standard is used in Antibiotic susceptibility test. Antimicrobial

susceptibility test is used to find the effective antimicrobial agent ( drugs /

antibiotics ) for successful treatment of an infection.

McFarland standard used in antimicrobial susceptibility testing for preparation of

microbial suspension of 1.5X108 numbers of bacteria. The performance of

Antimicrobial Sensitivity Disk Test require appropriate amount of bacterial

suspension which can be obtained by optically comparing turbidity of bacterial

suspension with 0.5 McFarland standard. If the bacterial suspension does not

appear to be the same density as the McFarland 0.5, the turbidity can be reduced

by adding sterile saline or increased by adding more microbial inoculum

(Transferring Bacterial Culture).

Reagent

0.5 mL of 1.175% barium chloride dihydrate (BaCl22H2O)

0.69.95 mL 1% sulfuric acid (H2SO4).

The standard can be compared visually to a suspension of bacteria in sterile

saline. If the bacterial suspension is too turbid, it can be diluted with more saline.

If the suspension is not turbid enough, more bacteria can be added.


Microxpress


PYR Reagent is used in conjunction with PYR testing for the detection of L-

pyroglutamyl aminopeptidase found in group A streptococci and group D

enterococci.

The PYR test uses L-pyrrolidonyl-beta-naphthlyamide substrate to detect the

presence of enzyme L-pyroglutamyl-aminopeptidase. Following hydrolysis of the

substrate by the enzyme the resulting beta-naphthylamine produces a cherry red

pigment upon the addition of PYR Reagent (cinnamaldehyde).

PYR ReagentReagent

PYR Reagent comprises of 1% cinnamaldehyde.

Cultural Response

Organism (ATCC) PYR test

E.faecalis (29212) +

S.bovis (27960) -

S.pyogenes (19615) +

Ready to use...


Nitrite detection strip and zinc dust are used to perform the nitrate reduction test.

Bacterial species may be differentiated on the basis of their ability to reduce

nitrate to nitrite or nitrogenous gases. Many bacteria have respiratory enzyme

systems that can use nitrate as a terminal electron acceptor forming nitrite.

Bacterial reduction of nitrate results in the production of various end products like

nitrite, nitrogen gas, ammonia, nitrous oxide and hydroxylamine. The nitrate

reduction characteristic of a particular species is more or less constant making it a

useful diagnostic tool.

The ability to reduce nitrate is valuable for differentiating and identifying various

types of bacteria, especially Enterobacteriaceae. Non-fermenters and other

miscellaneous gram negative bacilli vary in their ability to reduce nitrates. Some

members of this group are capable of denitrification which is a reduction of nitrate

to nitrogen gas. For the glucose fermenting gram negative bacilli, the production

of nitrogen gas is an important differential test. The end product of reduction

depends on bacterial species. Reduction of nitrate is generally an anaerobic

respiration process in which an organism derives its oxygen from nitrate.

This is a test for the presence of the enzyme nitrate reductase which causes the

reduction of nitrate, in the presence of a suitable electron donor, to nitrite which

can be tested for by an appropriate colorimetric reaction. The microorganism

under evaluation is inoculated in the medium and after incubation, nitrate

reduction can be determined. Upon addition of nitrite detection strip during the

test, development of pink, deep red or violet colour indicates a positive reaction.

No change in the colour of the strip only indicates that nitrite is not present in the

medium. There are two explanations for these observations:

Nitrite Detection Kit (General Microbiology)

1) The nitrate may not have been reduced and hence the strain is nitrate

negative.

2) The nitrate may have been reduced to nitrite, and further the nitrite has been

completely reduced to nitric oxide, nitrous oxide or nitrogen which will not

react with the reagents on the nitrite detection strip; the strain is nitrate

positive.

Any test medium that gives a negative result for the nitrite detection strip can be

further tested to determine which of the two interpretations is correct. This is done

by adding a small amount of zinc dust to all negative tests. The zinc dust will

catalyze the reduction of nitrate to nitrite. Thus, if the nitrate has not been reduced

by the organisms, i.e. the strain is nitrate negative, it will be reduced by the zinc

dust and the nitrite detection strip will show colour change from colourless to

pink/red/violet. If no colour change in the nitrite reduction strip is observed after

the addition of zinc dust, the organisms have not only reduced nitrate to nitrite,

but have further reduced nitrite to nitrogenous gases; the organisms are nitrate

positive. Almost all Enterobacteriaceae reduce nitrate.

Reagent

Nitrite Detection Kit comprises of

a. Nitrite Detection Strip

b. Zinc Dust

Cultural Response

Organism (ATCC) Nitrate reduction

E.aerogenes (13048) +

E.coli (25922) +

S.serotype typhimurium (14028) +



TDA Reagent (ferric chloride reagent) is used to visualize the phenylalanine

deamination reaction which allows differentiation of enteric bacilli on the basis of

their ability to produce phenylpyruvic acid by oxidative deamination. This reaction

is very useful in the taxonomy of Enterobacteriaceae.

The test allows to differentiate members of the Proteeae from other members of

the Enterobacteriaceae by the ability of organisms in the genera within the

Proteeae to deaminate phenylalanine to phenylpyruvic acid by enzymatic

activity. Proteus, Providencia and Morganella species possess this capability.

Phenylalanine serves as the substrate for enzymes, which are able to deaminate it

TDA Reagentto form phenylpyruvic acid. The addition of TDA Reagent following incubation

results in appearance of deep green color in case of positive reaction or no color

change in case of negative reaction.

Reagent

TDA Reagent comprises of 10% ferric chloride.

Cultural Response

Organism (ATCC) TDA test


P.alcalifaciens (9886) +

P.vulgaris (13315) +

As bacteria consists of clear protoplasmic matter, differing but slightly in refractive

index from the medium in which they are growing, it is difficult with the ordinary

microscope, except when special methods of illumination are used, to see them in

the unstained condition. Staining, therefore is of primary importance for the

recognition of bacteria.

Stains are used

lTo render microscopic and semitransparent objects visible.

lTo reveal their shape and size.

lTo show the presence of various internal and external structures.

lTo produce specific chemical and physical reactions.

Apart from differentiating and rendering visible the constituents of the cell, stains

will help to identify the organisms and classify to a particular group by their

individual reactions to particular stains. Broadly stains are made up of dyes which

can be classified as natural or synthetic.

The vast majority of staining procedures applied to microorganisms involves the

use of smears on glass slides. The essential steps in the preparation of a fixed,

stained smear are 1) preparation of the film or smear, 2) fixation and

decolourization, and 3) application of one or more staining solutions. The

advantages of these procedures are that the cells are made more clearly visible

after they are coloured and differences between cells of different species and

within the same species can be demonstrated by use of appropriate staining

solutions (differential or selective staining).

A large number of coloured organic compounds (dyes) are available for staining

microorganisms. These compounds are generally rather complex in terms of

Staining Solutionsmolecular structure and on this basis they may be classified into groups such as

triphenylmethane dyes, oxazine dyes and thiazine dyes.

A more practical classification for the cytologist is one based on the chemical

behaviour of the dye; namely acidic, basic or neutral i.e. the most commonly used

stains are salts composed of a base and an acid. An acid (or anionic) dye is the one

in which the charge on the dye ion is negative; a basic (or cationic) dye is one in

which the charge carried by the dye ion is positive. A neutral dye is a complex salt

of a dye acid with a dye base. Acid dyes generally stain basic cell components, and

basic dyes generally stain acidic cell components. (for eg. protoplasm of the

organism being acidic and negatively charged, will combine with the positively

charged cations or coloured part of the basic dye). The process of staining may

involve ion-exchange reactions between the stain and active sites at the surface of

or within the cell.

Many different kinds of staining solutions are employed in various bacteriological

procedures. Simple staining is carried out by applying a single solution of stain to

the fixed smear wherein the organisms are given the colour characteristic of the

staining solution. These stains bring out the morphology best. Differential stains

utilize more than one stain and make visible the differences between bacterial cell

or parts of a bacterial cell. These stains are necessary to bring out characteristics

like flagella, capsules, spores, metachromatic granules, polyssacharides and

lipids.

We offer microbiologists carefully standardized and reproducible staining

solutions in dropper bottles in order to ensure accurate and interpretable results.


to the package insert of the individual product.

Microxpress


Ready to use...

423

Microxpress



Gram Stain is used to differentiate between gram-positive and gram-negative

bacteria depending on their ability to take up either the primary stain or the

counter stain.

Various mechanisms have been proposed to explain the gram reaction. Most

researchers who have studied the mechanism of gram stain reaction have

concluded that the mechanism is based on the distinctive chemistry and physical

structure of the cell walls of the organisms (there are many physiological

differences between the cell walls of gram-positive and gram-negative bacteria).

Certain bacteria when treated with one of the basic para-rosanaline dye such as

crystal violet (primary stain), and then iodine, 'fix' the stain so that subsequent

treatment with a decolourizing agent like alcohol does not remove the colour.

Other organisms, however, are decoulorized by this process. If a mixture of various

organisms are thus stained and subjected to the decolourizer, some organisms

retain the dye and turn violet in colour-these are termed as gram positive,

whereas others that are completely decoulorized are termed gram negative. In

order to render the decoulorized organisms visible, and to distinguish them from

those retaining the colour, a counter stain like methyl red or safranine is applied.

These organisms take up the stain methyl red and turn red in colour.

Generally, a thin smear of bacterial cell suspension is stained with crystal violet,

then treated with an iodine containing mordant to increase the binding of the

primary stain. A decolourizing solution of alcohol is used to remove the crystal

violet from cells which bind it weakly and then a counterstain like safranin is used

to provide a colour contrast in those cells that are decolourized.

The gram positive characteristic results from the ability of a cell to resist the loss of

the primary stain during the decolourization step, as compared to the gram

negative cells which do not possess this ability. The explanation of this

phenomenon is associated with the composition and structure of the cell wall.

Gram Stain KitThe gram-positive organisms have more mucopeptide in their cell walls as

compared to gram-negative ones. On the other hand, gram-negative bacteria

have a high content of lipids in their cell walls. Cell walls of gram-positive

organisms also contain teichoic acids in abundance, not seen in cell walls of

gram-negative organisms. Experimental evidence suggest that during staining of

gram negative bacteria, the alcohol treatment extracts the lipids, which results in

increased porosity or permeability of the cell wall. Thus the crystal violet-iodine

(CV-I) complex can be extracted and the gram negative organism is decolourized,

which subsequently take up the colour of the counter stain. The gram positive

bacteria, because of their lower lipid content become dehydrated during alcohol

treatment. The pore size is decreased, permeability is reduced and the CV-I

complex cannot be extracted. Therefore these cells remain purple violet and do not

take up the counterstain.

The gram stain has its greatest use in characterizing bacteria.

Reagent

Gram Stain Kit is provided as a four component reagent.

Gram’s Crystal Violet solution (Reagent A)

2% crystal violet.

Gram’s Iodine-Iodide solution (Reagent B)

0.66% potassium iodide and 0.33% iodine

Games Decolorizer solution (Reagent C )

50% Acetone in 95% Ethanol.Gram’s Safranine solution (Reagent D)

0.25% safranine.

Observation

Gram positive organisms stain purple in colour whereas gram negative organisms

stain red in colour.

Lactophenol Cotton BlueSummary and Principle

Lactophenol Cotton Blue wet mount preparation is the most widely used method

of staining and observing fungi. It is formulated with lactophenol, which serves as

the mounting fluid and the dye, cotton blue. Lactic acid preserves the fungal

structure and clears the tissue while phenol acts as a disinfectant. The high

concentration of phenol deactivates lytic cellular enzymes thus the cells do not

lyse. Cotton blue is an acid dye that stains the chitin present in the cell walls. The

stain imparts blue colour on fungal hyphae.

Reagent

Lactophenol Cotton Blue comprises of

20% phenol, 0.05% cotton blue, 20% lactic acid in glycerol

Phenol 20 gms

Cotton blue 0.05 gms

Lactic acid 20 ml

Glycerol 40 ml

Distilled water 100 ml

Observation

Fungal hyphae stain blue in colour.

Ready to use...

424

Microxpress


Ready to use...


Lactophenol Picric Acid is used for staining as well as for wet mounting of fungi.

Picric acid is a nitrophenol.

Reagent

Lactophenol Picric Acid comprises of

Phenol 100 gms

Lactophenol Picric AcidLactic acid 100 ml

Glycerol 200 ml

Picric acid, saturated aqueous solution 100 ml

Observation

Fungal spores and hyphae stain pale to dark yellow.


Picric acid is used for staining of fungi. It is a 2,4,6-trinitrophenol having

antibacterial as well as antifungal properties and stains the fungal spores pale to

dark yellow.

Reagent

Picric Acid comprises of

Picric AcidPicric acid 1.18 gms

Distilled water 100 ml

Observation

Fungal spores stain pale to dark yellow.

425

APPLICATIONS

Food and Beverage Analysis

PRODUCTS

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Alkaline Peptone water

Azide Dextrose Broth (AM50061)

Bacillus Cereus Agar Base (AM1009/AM5009)

Baird Parker Agar Base (AM1011/AM5011)

BP Sulpha Supplement* (AS005)

Potassium Tellurite 3.5%* (AS023)

Bile Esculin Agar (AM1012/AM5012)

Bismuth Sulphite Agar (AM1013/AM5013)

Blood Agar Base (AM1014/AM5014)

Brain Heart Infusion Agar (AM1016/AM5016)

Brain Heart Infusion Broth (AM1017/AM5017)

Brilliant Green Agar, Modified (AM1018/AM5018)

Sulpha Supplement* (AS027)

Brilliant Green Bile Broth 2% (AM1020/AM5020)

Chloramphenicol Yeast Glucose Agar*

Colombia Blood Agar Base (AM1029/AM5029)

Staph-Strepto Supplement* (AS025)

Strepto Supplement* (AS026)

Campylobacter agar Base (AM50218)

Cooked Meat Medium (AM1030/AM5030)

DNase Test Agar Base (AM10381)

Deoxycholate Citrate Agar (AM1031/AM5031)

Dextrose Tryptone Agar (AM1036/AM5036)

Dextrose Tryptone Broth (AM1037/AM5037)

(AM1910/AM5911)

(AM1024/AM5024)

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APPLICATIONS


PRODUCTSDifferential Reinforced Clostridial Broth (AM1038/AM5038)

EC Broth (AM1039/AM5039)

Fraser Broth Base (AM50456)

(AM104821/AM504821)

(AM50532)

(AM10576/AM50576)

(AM1058/AM5058)

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EMB Agar,Levine (AM1040/AM5040)

Fluid Lactose Medium (AM1042/AM5042)

Fluid Sabouraud Medium (AM1043/AM5043)

Fluid Selenite Cystine Medium (AM1044/AM5044)

Fluid Thioglycollate Medium (AM1045/AM5045)

Heart Infusion Agar (AM10481/AM50481)

Heart Infusion Broth (AM10482/AM50482)

Iron Sulphite Agar (AM10483/AM50483)

Karmali Campylobacter Agar Base (AM1049/AM5049)

Campylobacter Selective Supplement with Hemin (Karmali), Modified* (AS007)

Kligler Iron Agar (AM1050/AM5050)

Lactobacilli MRS Agar (AM1051/AM5051)

Lactose Broth (AM1052/AM5052)

Lauryl Tryptose Broth (AM1053/AM5053)

Listeria Identification Agar Base, PALCAM (AM1055/AM5055)

Listeria Selective Supplement *(AS018)

LPM Agar Base (AM10575)

Lysine Iron Agar

MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts

Hektoen Enteric Agar

Lee's Agar



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troph

ic O

rgan

ism

sS

alm

onel

la

Shi

gella

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Tota

l Pla

te C

ount

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia e

nter

ocol

itica

Mes

ophi

lic S

pore

form

ers

(Ana

erob

ic)

Iron

Sul

phite

spo

refo

rmer

s

Pro

teol

ytic

mic

roor

gani

sms

Aci

d P

rodu

cing

Bic

robi

olog

y

Aer

ogen

e

Lact

obac

illus

Ent

eroc

occu

s fa

ecal

is

APPLICATIONS




Fraser Broth Base (AM50456)

(AM104821/AM504821)

(AM50532)

(AM10576/AM50576)

(AM1058/AM5058)

l

l

l l

l

ll

l

l

l l

l l

l

l

l

l l l

l

l

l

l

l

l

l

l ll l

EMB Agar,Levine (AM1040/AM5040)






Heart Infusion Broth (AM10482/AM50482)


Karmali Campylobacter Agar Base (AM1049/AM5049)

Campylobacter Selective Supplement with Hemin (Karmali), Modified* (AS007)


Lactobacilli MRS Agar (AM1051/AM5051)



Listeria Identification Agar Base, PALCAM (AM1055/AM5055)

Listeria Selective Supplement *(AS018)


Lysine Iron Agar

MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts

Hektoen Enteric Agar

Lee's Agar



Bac

illus

cer

eus

Aci

duric

and

The

rmop

hilic

Fl

at S

our S

pore

form

ers

Cam

pylo

bact

erC

lost

ridiu

m

Col

iform

s

List

eria

Mes

ophi

lic S

pore

form

ers

(Aer

obic

)

Osm

ophi

lic o

rgan

ism

s

Psy

chro

troph

ic O

rgan

ism

sS

alm

onel

la

Shi

gella

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Tota

l Pla

te C

ount

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia e

nter

ocol

itica

Mes

ophi

lic S

pore

form

ers

(Ana

erob

ic)

Iron

Sul

phite

spo

refo

rmer

s

Pro

teol

ytic

mic

roor

gani

sms

Aci

d P

rodu

cing

Bic

robi

olog

y

Aer

ogen

e

Lact

obac

illus

Ent

eroc

occu

s fa

ecal

is

APPLICATIONS


PRODUCTSMacConkey Broth with Neutral Red (AM1064/AM5064)

MacConkey Broth Purple with Bromocresol Purple (AM1063/AM5063)

(AM10635/AM50635)

MacConkey Broth Harmonized (AM5063)

MacConkey Broth USP (AM50636)

Malonate Broth Ewing Modified (AM1065/AM5065)

Malt Agar (AM1066/AM5066)

Malt Extract Agar (AM1067/AM5067)

Malt Extract Broth (AM1068/AM5068)

Mannitol Motility Test Medium (AM10681/AM50681)

Modified Rappaport Vassiliadis Medium(AM506928)

Orange Serum Broth (AM50783)

l

l

l

l l

l

l

l ll

l

l l

l

l

l

l

l

l

l l l ll l

l

l ll

l

l l ll

l

l

MacConkey Broth Purple with BCP ISO/BIS/BP/IP

MacConkey Broth

Modified Rappaport Vassiliadis Medium for Water

Testing BIS/ISO (AM506929/30)

MUG Lauryl Sulphate Broth (AM50721)

Mannitol Salt Agar (AM1069/AM5069)

Motility Test Medium (AM506933)

MR-VP Medium (AM1071/AM5071)

Nutrient Agar (AM1074/AM5074)

Nutrient Agar pH 6.8 (AM1075/AM5075)

Nutrient Broth (AM1077/AM5077)

Orange Serum Agar (AM50782)



APPLICATIONS


PRODUCTSPhenol Red Dextrose Broth (AM50802)

Phenol Red Lactose Broth (AM50802)

Phenol Red Sucrose Broth (AM50808)

Plate Count Agar (AM1081/AM5081)

PNY Medium (AM10812/AM50812)

Potato Dextrose Agar (AM1082/AM5082)

Potato Dextrose Broth (AM1083/AM5083)

Potato Dextrose Agar USP (AM108213/AM50821)

Raka-Ray Agar Base

(Lactic Acid Bacteria Selective Agar Base)(AM10844)

Raka-Ray No. 3 Broth Base

(Lactic Acid Bacteria Selective Broth Base)(AM10845)

Reddy's Differential Agar, Modified (AM50846)

Reinforced Clostridial Agar (AM1085/AM5085)

Rose Bengal Chloramphenicol Agar (AM10857/AM50857)

Sabouraud Dextrose Agar (AM1087/AM5087)

Sabouraud Dextrose Broth (AM1088/AM5088)

Salt Meat Broth (AM50886)

Selenite F Broth (AM1089/AM5089)

Simmons Citrate Agar (AM1090/AM5090)

Skim Milk Agar (AM10901/AM50901)

Soyabean Casein Digest Agar (AM1091/AM5091)

Soyabean Casein Digest Medium (AM1092/AM5092)

SS Agar (AM1093/AM5093)

l

l

l

l ll l l

l

ll l l

l

l

l

l

l

l

l

l

l

l

l

lll

l

l l l l l l

l l lll l

ll

Bac

illus

cer

eus

Aci

duric

and

The

rmop

hilic

Fl

at S

our S

pore

form

ers

Cam

pylo

bact

erC

lost

ridiu

m

Col

iform

s

List

eria

Mes

ophi

lic S

pore

form

ers

(Aer

obic

)

Osm

ophi

lic o

rgan

ism

s

Psy

chro

troph

ic O

rgan

ism

sS

alm

onel

la

Shi

gella

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Tota

l Pla

te C

ount

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia e

nter

ocol

itica

Mes

ophi

lic S

pore

form

ers

(Ana

erob

ic)

Iron

Sul

phite

spo

refo

rmer

s

Pro

teol

ytic

mic

roor

gani

sms

Aci

d P

rodu

cing

Bic

robi

olog

y

Aer

ogen

e

Lact

obac

illus

Ent

eroc

occu

s fa

ecal

is



APPLICATIONS


PRODUCTSStandard Nutrient Agar /Broth (AM509311/12)

TCBS Agar (AM1095/AM5095)

Tetrathionate Brilliant Green Bile Broth (AM50954)

Tetrathionate Broth Base, Hajna (AM1096/AM5096)

Triple Sugar Iron (TSI) Agar (AM1099/AM5099)

Tryptone Glucose Extract Agar (AM1101/AM5101)

Tryptone Glucose Extract Broth (AM1102/AM5102

Tryptone Phosphate Broth (AM1103/AM5103)

Tryptone Water (AM1104/AM5104)


TCBS Agar (AM1095)

Universal Beer agar (AM11042)

Urea Broth Base (AM1106/AM5106)

Urea 40%* (AS028)

Violet Red Bile Agar (AM1107/AM5107)

Violet Red Bile Agar with MUG (AM510712)

Violet Red Bile Broth (AM51072)

Violet Red Bile Glucose Agar (Harmonized)(AMH51073)

Vogel Johnson Agar Base (AM1108/AM5108)

Potassium Tellurite 1%* (AS022)

XLD Agar (AM1112/AM5112)

Yeast Extract Agar (AM1113/AM5113)

Yeast Malt Agar (AM1114/AM5114)

Yersinia Selective Agar Base (AM1116/TM116005)

l l

l

l

l

l l l ll l

l l

l

l

l l

ll

l

l

l l ll

l

l

l l l

l l l

l

ll

l

l

l

Bac

illus

cer

eus

Aci

duric

and

The

rmop

hilic

Fl

at S

our S

pore

form

ers

Cam

pylo

bact

erC

lost

ridiu

m

Col

iform

s

List

eria

Mes

ophi

lic S

pore

form

ers

(Aer

obic

)

Osm

ophi

lic o

rgan

ism

s

Psy

chro

troph

ic O

rgan

ism

sS

alm

onel

la

Shi

gella

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Tota

l Pla

te C

ount

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia e

nter

ocol

itica

Mes

ophi

lic S

pore

form

ers

(Ana

erob

ic)

Iron

Sul

phite

spo

refo

rmer

s

Pro

teol

ytic

mic

roor

gani

sms

Aci

d P

rodu

cing

Bic

robi

olog

y

Aer

ogen

e

Lact

obac

illus

Ent

eroc

occu

s fa

ecal

is



APPLICATIONS


PRODUCTS

Bac

illus

cer

eus

Aci

duric

and

The

rmop

hilic

Fl

at S

our S

pore

form

ers

Cam

pylo

bact

erC

lost

ridiu

m

Col

iform

s

List

eria

Mes

ophi

lic S

pore

form

ers

(Aer

obic

)

Osm

ophi

lic o

rgan

ism

s

Psy

chro

troph

ic O

rgan

ism

sS

alm

onel

la

Shi

gella

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Tota

l Pla

te C

ount

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia e

nter

ocol

itica

Mes

ophi

lic S

pore

form

ers

(Ana

erob

ic)

Iron

Sul

phite

spo

refo

rmer

s

Pro

teol

ytic

mic

roor

gani

sms

Aci

d P

rodu

cing

Bic

robi

olog

y

Aer

ogen

e

Lact

obac

illus

Ent

eroc

occu

s fa

ecal

is

Yersinia Selective Supplement *(AS029)

W.L. Differential Broth (AM51091)

W L Nutrient Agar (AM51092)

l

l

lW L Nutrient Broth (AM1110/AM5111)



Dairy Analysis

Bac

illus

Spe

cies

(and

oth

er s

pore

be

arer

s)

APPLICATIONS

PRODUCTS

Col

iform

s

Ana

erob

ic

Mic

roor

gani

sms

(Incl

udin

g C

lost

ridia

)

Lact

obac

illi

List

eria

m

onoc

ytog

enes

Sal

mon

ella

/Shi

gella

Sta

ndar

d P

late

C

ount

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Yeas

t & M

ould

Yers

inia

ent

eroc

oliti

ca

Cam

pylo

bact

er

Pro

teol

ytic

act

ivity

AK Agar No. 2 (AM5909)

Azide Blood Agar Base (AM1006/AM5006)


Bacillus Cereus Agar Base (AM1009/5009)

Polymyxin B Selective Supplement* (AS021)

Egg Yolk Supplement* (AS010)


B P Sulpha Supplement* (AS005)

Egg Yolk Emulsion* (A010)

Egg Yolk Tellurite Emulsion* (AS011)






Brilliant Green Agar Modified (AM1018/AM5018)



Campylobacter Agar Base (AM50218)

Chloramphenicol Yeast Glucose Agar (AM1024/AM5024)


Czpek Dox Agar (AM10302/AM50302)

Decarboxylase Agar Base (AM503023)

Deoxycholate agar (AM10303/AM50303)

l

l

l

l

l

l

l

l

l l

l

l

l

l

l

l

l

l

l

Buffered Peptone Water (AM10211/50211)

Ent

erob

acte

r and

K

lebs

iella

spe

cies

Pse

udom

onas

ae

rugi

nosa

,

Ser

ratia

mar

cesc

ens

Clo

strid

ia

Pro

teus

mira

bilis

Ent

eroc

occu

s fa

ecal

is

Aer

ugin

osa



Bac

illus

Spe

cies

(and

oth

er s

pore

be

arer

s)

Col

iform

s

Ana

erob

ic

Mic

roor

gani

sms

(Incl

udin

g C

lost

ridia

)

Lact

obac

illi

List

eria

m

onoc

ytog

enes

Sal

mon

ella

/Shi

gella

Sta

ndar

d P

late

C

ount

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Yeas

t & M

ould

Yers

inia

ent

eroc

oliti

ca

Cam

pylo

bact

er

Pro

teol

ytic

act

ivity

Ent

erob

acte

r and

K

lebs

iella

spe

cies

Pse

udom

onas

ae

rugi

nosa

,

Ser

ratia

mar

cesc

ens

Clo

strid

ia

Pro

teus

mira

bilis

Ent

eroc

occu

s fa

ecal

is

Aer

ugin

osa

Dairy Analysis



EMB Agar, Levine (AM1040/AM5040)

Endo Agar (AM1040/AM5040)



Hektoen Enteric Agar (AM104821/AM504823)


Lactobacillus MRS Agar (AM1051/AM5051)



Lysine Iron Agar (AM10576/AM50576)

MacConkey Broth Harmonized (AMH5063)


MacConkey Broth Purple with BCP ISO/BIS/BP/IPBCP

l

l

l

l

l l

l

l

l

l

l

l

l

l

l

l

l

l

l

l

l

l

l l l

l

l

Lactobacillus MRS Broth (AM10512)


Lee's Agar (AM50532)

Listeria Identification Agar Base, PALCAM, (AM1055/AM5055)

Listeria Oxford Medium Base (AM105512/AM505512)

Oxford Listeria Supplement* (AS0201)

Listeria Moxlactam Supplement* (AS0171)

Listeria Selective Supplement* (AS018)

MacConkey Agar

MacConkey Agar with Crystal Violet, NaCl and 0.15%Bile Salts (AM1059/AM5059)

MacConkey Broth with Bromocresol Purple (AM1063/AM5063)

APPLICATIONS



Bac

illus

Spe

cies

(and

oth

er s

pore

be

arer

s)

Col

iform

s

Ana

erob

ic

Mic

roor

gani

sms

(Incl

udin

g C

lost

ridia

)

Lact

obac

illi

List

eria

m

onoc

ytog

enes

Sal

mon

ella

/Shi

gella

Sta

ndar

d P

late

C

ount

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Yeas

t & M

ould

Yers

inia

ent

eroc

oliti

ca

Cam

pylo

bact

er

Pro

teol

ytic

act

ivity

Ent

erob

acte

r and

K

lebs

iella

spe

cies

Pse

udom

onas

ae

rugi

nosa

,

Ser

ratia

mar

cesc

ens

Clo

strid

ia

Pro

teus

mira

bilis

Ent

eroc

occu

s fa

ecal

is

Aer

ugin

osa

Dairy Analysis

PRODUCTSMalonate Broth, Ewing Modified (AM1065/AM5065)




Milk Agar (AM10692711/AM50692711)

Modified Rappaport Vassiliadis Medium for Water Testing BIS/ISO (AM506929/AM506930)



Nitrate Broth




Phenylalanine Agar (AM108091/AM508091)



Preston Selective Supplement (Campylobacter Selective Supplement IV, Modified) (AS231)

Reddy's Differential Agar Modified (AM50846)


Reinforced Clostridial Broth

Rogosa SL Agar (AM50854)

Rogosa SL Broth (AM50855)

Rose Bengal Agar Base (AM50856)

Rose Bengal Chloramphenicol Agar (AM10857/AM50857)

SIM Medium (AM50892)

Simmons Citrate Agar (AM1090/5090)

l l

l

l

l

l l l l l l

l

l

l l l

l l

l l

l

l

l

l

l

l

l

l

l

l

l

l

ll l

l l

APPLICATIONS



Bac

illus

Spe

cies

(and

oth

er s

pore

be

arer

s)

Col

iform

s

Ana

erob

ic

Mic

roor

gani

sms

(Incl

udin

g C

lost

ridia

)

Lact

obac

illi

List

eria

m

onoc

ytog

enes

Sal

mon

ella

/Shi

gella

Sta

ndar

d P

late

C

ount

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Yeas

t & M

ould

Yers

inia

ent

eroc

oliti

ca

Cam

pylo

bact

er

Pro

teol

ytic

act

ivity

Ent

erob

acte

r and

K

lebs

iella

spe

cies

Pse

udom

onas

ae

rugi

nosa

,

Ser

ratia

mar

cesc

ens

Clo

strid

ia

Pro

teus

mira

bilis

Ent

eroc

occu

s fa

ecal

is

Aer

ugin

osa

Dairy Analysis

Skim Milk Agar (AM10901/AM50901)



Soyabean Casein Digest Agar with Lecithin and Tween 80*


Staphylococcus Agar No.110 (AM10932/AM50932)


Tetrathionate Broth Base

Tomato Juice Agar (AM1097/AM5097)

Tomato Juice Agar, Special (AM1098/AM5098)


Tryptone Glucose Beef Extract Agar (TGB Agar)(AM1102/AM5102)



Tryptone Soya Agar with Lecithin and Tween 80* (AM11031/AM51031)


Urea Agar Base, Christensen (AM1105/AM5105)


Urea 40%* (AS028)

Violet Red Bile Agar Base (AM1107/AM5107)

Violet Red Bile Agar with MUG (AM510712)

Violet Red Bile Glucose Agar (Harmonized) (AMH51073)


l ll ll l

l ll

l

l

l

l

l

l

l

l

l l l

l

l

l

l

l l

l l l

l l

l

ll l

l l l l

l

(AM50927)

Urea 40%* (AS028)

PRODUCTS

APPLICATIONS



Bac

illus

Spe

cies

(and

oth

er s

pore

be

arer

s)

Col

iform

s

Ana

erob

ic

Mic

roor

gani

sms

(Incl

udin

g C

lost

ridia

)

Lact

obac

illi

List

eria

m

onoc

ytog

enes

Sal

mon

ella

/Shi

gella

Sta

ndar

d P

late

C

ount

Sta

phyl

ococ

cus

Stre

ptoc

occu

s/E

nter

ococ

cus

Yeas

t & M

ould

Yers

inia

ent

eroc

oliti

ca

Cam

pylo

bact

er

Pro

teol

ytic

act

ivity

Ent

erob

acte

r and

K

lebs

iella

spe

cies

Pse

udom

onas

ae

rugi

nosa

,

Ser

ratia

mar

cesc

ens

Clo

strid

ia

Pro

teus

mira

bilis

Ent

eroc

occu

s fa

ecal

is

Aer

ugin

osa

Dairy Analysis

PRODUCTS

APPLICATIONS



Yeast Extract Chloramphenicol Dextrose Agar (AM51131)


Yersinia Selective Supplement* (AS029)

l

l

l



Ant

ibio

tic A

ssay

M

edia

E. c

oli

Pse

udom

onas

Sal

mon

ella

e

Sta

phyl

ococ

cus

Tota

l Aer

obic

M

icro

bial

Cou

nt

Yeas

t and

Mou

ld

Cou

nt

Ste

rility

Tes

ting

Vita

min

Ass

ay

Lact

obac

illus

Cou

nt

Microbial Limit Test

Pharmaceutical Analysis

PRODUCTSAlternative Thioglycollate Medium (AM1900/AM5900)

Antibiotic Assay Medium A (No. 1, Seed Agar) (AM1002/AM5002)

Antibiotic Assay Medium C (No. 3, Assay Broth) (AM1003/AM5003)

Antiobiotic Assay Medium E (No. 5) (Streptomycin Assay agar with yeast Extarct) (AM50031)

Antiobiotic Assay Medium F (No. 8) (Base Agar with low pH)

Antibiotic Assay Medium No 11 (Neomycin, Erythromycin Assay Agar) (AM1004/AM5004)

Antibiotic Assay Medium G (N. 19) (AM500411)

Antiobiotic Assay Medium No. 32 (AM500412)

Antifungal Assay Agar (AM10041/AM50041)

B12 Assay Agar using E.coli Mutant Culture (AM1007/AM5007 )

B12 Assay Medium (AM10071)

B12 Maintenance Medium for E.coli Mutant (AM1008/AM5008)



Egg Yolk Emulsion* (AS010)






Cetrimide Agar Base (AM1022/AM5022)

Columbia agar Base

Nalidixic Selective Supplement* (AS020)


E.E. Broth, Mossel (AM103913/AM503913)


l

l

l

l

l

l

l

l

l

l

l

l

l

l

(AM50032)

APPLICATIONS




APPLICATIONS

PRODUCTS

Ant

ibio

tic A

ssay

M

edia

E. c

oli

Pse

udom

onas

Sal

mon

ella

e

Sta

phyl

ococ

cus

Tota

l Aer

obic

M

icro

bial

Cou

nt

Yeas

t and

Mou

ld

Cou

nt

Ste

rility

Tes

ting

Vita

min

Ass

ay

Lact

obac

illus

Cou

nt





Glucose Yeast Extract Agar (AM1048/AM5048)

Inactivator Broth (AM104824/AM504824)


Lactose Sulphite Broth Base (AM50525)


MacConkey Agar with Crystal Violet,NaCl and 0.15% Bile Salts (AM1059/AM5059)

MacConkey Broth

(AM10785)

(AM10787)

l l

l

l

l

l

l

l

l

l

l

l l

l

l

l

l

l

l

l

l ll l

MacConkey Broth with Bromocresol Purple (AM1063/AM5063)



Pantothenate Assay Medium (AM10784)

Pantothenate Assay Medium, AOAC

Pantothenate Inoculum Broth

Pseudomonas Agar (For Fluorescein) (AM108411/AM508411)

Pseudomonas Agar (For Pyocyanin) (AM108414)

Peptone Water (AM1079/AM5079)


Sabouraud Chloramphenicol Agar (AM1086/AM5086)









APPLICATIONS

PRODUCTS

Ant

ibio

tic A

ssay

M

edia

E. c

oli

Pse

udom

onas

Sal

mon

ella

e

Sta

phyl

ococ

cus

Tota

l Aer

obic

M

icro

bial

Cou

nt

Yeas

t and

Mou

ld

Cou

nt

Ste

rility

Tes

ting

Vita

min

Ass

ay

Lact

obac

illus

Cou

nt


Soyabean Casein Digest Medium Sterile Powder (AM50925/AM50925-5K)

Tetrathionate Brilliant Green Bile Broth (AM50954)



Urea Broth Base (AM1105)

Urea 40%* (AS028)


Potassium Tellurite 1%*(AS022)

W.L. Differential Broth (AM51091)


l l l

l

l

l

l

l

l



APPLICATIONS

Water and Wastewater Analysis

PRODUCTS Tota

l Pla

te C

ount

Sta

phyl

ococ

cus

MP

N C

onfir

med

MP

N P

resu

mpt

ive

Iden

tific

atio

n &

B

ioch

emic

al T

ests

Sel

ectiv

e Is

olat

ion

Pre

-Enr

ichm

ent/

Sel

ectiv

e E

nric

hmen

t

Pse

udom

onas

Legi

onel

la S

peci

es

Kle

bsie

lla

Feca

l Stre

ptoc

occi

Col

ipha

ge D

etec

tion

Diff

eren

tiatio

n of

Fec

al &

Non

feca

l Col

iform

Feca

l Col

iform

s

MP

N C

ompl

eted

Tes

t

MP

N C

onfir

med

Tes

t

MP

N P

resu

mpt

ive

Test

Clo

strid

ia

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia E

nter

ocol

itica

Total coliformSalmonella/Shigella

Qualitative Tests Quantitative Tests

Alkaline Peptone water (AM1910/AM5910)

Aspargine Proline Broth (AM1005/AM5005)





Egg Yolk Emulsion* (A010)

EY Tellurite Enrichment* (AS011)


Beef Extract Agar (AM10111/AM50111)

Bile Esculin Agar Base (AM1012/AM5012)

Bile Esculine Azide Agar (AM501212)









Cetrimide Broth (AM1023/AM5023)

Cetrimide Broth (Am1023/AM5023)

Corn Meal Agar (AM10301/AM50301)


l

l

l l

l

l

l

l

l

l

l

l l

ll

l

l

l

l

Cam

pylo

bact

er



APPLICATIONS


Tota

l Pla

te C

ount

Sta

phyl

ococ

cus

MP

N C

onfir

med

MP

N P

resu

mpt

ive

Iden

tific

atio

n &

B

ioch

emic

al T

ests

Sel

ectiv

e Is

olat

ion

Pre

-Enr

ichm

ent/

Sel

ectiv

e E

nric

hmen

t

Pse

udom

onas

Legi

onel

la S

peci

es

Kle

bsie

lla

Feca

l Stre

ptoc

occi

Col

ipha

ge D

etec

tion

Diff

eren

tiatio

n of

Fec

al &

Non

feca

l Col

iform

Feca

l Col

iform

s

MP

N C

ompl

eted

Tes

t

MP

N C

onfir

med

Tes

t

MP

N P

resu

mpt

ive

Test

Clo

strid

ia

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia E

nter

ocol

itica



Differential Reinforced Clostridial Broth (AM1038/AM5038)


ECD Agar (AM503912)



(AM5063)

l

ll

l l

l

l

l l

l

l

l l

l

ll l

l

l

l

l

l l






Legionella Agar Base (AM1054/AM5054)

Legionella Growth Supplement* (AS016)

Legionella Selective Supplement* (AS017)


MacConkey Agar with Crystal Violet, NaCl and with 0.15% Bile Salts (AM1059/AM5059)

MacConkey Agar without Crystal Violet and with 0.15% Bile Salts(AM1060/AM5060)

MacConkey Agar without Crystal Violet, NaCl and with 0.5% Sodium Taurocholate (AM1061/AM5061)

MacConkey Broth Double Strength with Neutral Red BIS(AM10621/AM50621)

MacConkey Broth Harmonized


MacConkey Broth with Neutral Red (AM1064/AM5064)

MacConkey Broth Purple W/Bromocresol Purple (AM1063/AM5063)

PRODUCTS

Cam

pylo

bact

er



APPLICATIONS


PRODUCTS Tota

l Pla

te C

ount

Sta

phyl

ococ

cus

MP

N C

onfir

med

MP

N P

resu

mpt

ive

Iden

tific

atio

n &

B

ioch

emic

al T

ests

Sel

ectiv

e Is

olat

ion

Pre

-Enr

ichm

ent/

Sel

ectiv

e E

nric

hmen

t

Pse

udom

onas

Legi

onel

la S

peci

es

Kle

bsie

lla

Feca

l Stre

ptoc

occi

Col

ipha

ge D

etec

tion

Diff

eren

tiatio

n of

Fec

al &

Non

feca

l Col

iform

Feca

l Col

iform

s

MP

N C

ompl

eted

Tes

t

MP

N C

onfir

med

Tes

t

MP

N P

resu

mpt

ive

Test

Clo

strid

ia

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia E

nter

ocol

itica



Cam

pylo

bact

er

MacConkey Broth Purple with BCP ISO/BIS/BP/IPBCP

Malonate Broth Ewing, Modified (AM1065/AM5065)

Malt Agar (AM1066/5066)

Malt Extract Agar (AM1067/5067)

Mannitol Motility Test Medium (AM10681/AM50681)


M-Endo Agar LES (AM106921/AM506921)

M-Endo Broth (AM106922/AM506922)

M-FC Agar Base (AM506923)

M-FC Broth Base (AM506924)

M-(HPC) Heterotrophic Plate Count Agar Base (AM506925)

M-(HPC) Heterotrophic Plate Count Broth Base (AM506926)


Milk Agar AM10692711/AM50692711)

MUG Lauryl Sulphate Broth (AM50721)

Nutrient Agar pH 6.8 (AM1075/AM5075)


PA Broth (AM507831)

Phenol Red Dextrose Broth (AM50802)


Phenol Red Mannitol Broth (AM50806)


Phenol Red Xylose Broth (AM50809)

Phenylalanine Agar (AM108091/AM508091)

l

l

l

l l l

l

l l l

l

l

l

l



APPLICATIONS


PRODUCTS Tota

l Pla

te C

ount

Sta

phyl

ococ

cus

MP

N C

onfir

med

MP

N P

resu

mpt

ive

Iden

tific

atio

n &

B

ioch

emic

al T

ests

Sel

ectiv

e Is

olat

ion

Pre

-Enr

ichm

ent/

Sel

ectiv

e E

nric

hmen

t

Pse

udom

onas

Legi

onel

la S

peci

es

Kle

bsie

lla

Feca

l Stre

ptoc

occi

Col

ipha

ge D

etec

tion

Diff

eren

tiatio

n of

Fec

al &

Non

feca

l Col

iform

Feca

l Col

iform

s

MP

N C

ompl

eted

Tes

t

MP

N C

onfir

med

Tes

t

MP

N P

resu

mpt

ive

Test

Clo

strid

ia

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia E

nter

ocol

itica



Cam

pylo

bact

er


Preston Agar Base (AM10831/AM50831)

Preston Selective Supplement (Campylobacter Selective Supplement IV, Modified) (AS0231)


Pseudomonas Agar (For Pyocyanin) (AM108411/AM508411)

Pseudomonas Agar Base (AM1084/AM5084)

Cetrinix Supplement* (AS008)

CFC Supplement* (AS009)

Pseudomonas Asparagine Broth (AM108418/AM508418)

R-2A Agar (AM10841/AM50841)

R-2A Agar (Agar Medium S)

Rappaport Vassiliadis Salmonella Enrichment Broth

(Harmonized) (AMH508453)



SIM Medium (AM50892)

Simmions Citrate Agar (AM1090/AM5090)





Tergitol-7 Agar Base (AM10951/AM50951)

Tergitol-7 Broth (AM50953)

l

l

l

l

l

l

l

l l

l l l

l

l l

l

l



APPLICATIONS


PRODUCTS Tota

l Pla

te C

ount

Sta

phyl

ococ

cus

MP

N C

onfir

med

MP

N P

resu

mpt

ive

Iden

tific

atio

n &

B

ioch

emic

al T

ests

Sel

ectiv

e Is

olat

ion

Pre

-Enr

ichm

ent/

Sel

ectiv

e E

nric

hmen

t

Pse

udom

onas

Legi

onel

la S

peci

es

Kle

bsie

lla

Feca

l Stre

ptoc

occi

Col

ipha

ge D

etec

tion

Diff

eren

tiatio

n of

Fec

al &

Non

feca

l Col

iform

Feca

l Col

iform

s

MP

N C

ompl

eted

Tes

t

MP

N C

onfir

med

Tes

t

MP

N P

resu

mpt

ive

Test

Clo

strid

ia

Vib

rio

Yeas

ts a

nd M

ould

sYe

rsin

ia E

nter

ocol

itica



Cam

pylo

bact

er


Triple Sugar Iron Agar (AM1099/AM5099)

Tryptone Glucose Beef Extract Agar (TGB Agar) (AM11002/AM51002)




Urea 40%* (AS028)


Urea 40%* (AS028)


Violet Red Bile Broth (AM51072)



Yeast Malt Broth (AM1115/AM5115)



l l

l l l l

l

l l

l l

l

l

l

l l

l

l

l



APPLICATIONS

Veterinary Testing

Yeas

t & M

ould

s

Vib

rio

Stre

ptoc

occi

Sta

phyl

ococ

ci

Sal

mon

ella

/Shi

gella

Pse

udom

onas

Myc

obac

teriu

m

List

eria

Gen

eral

Pur

pose

Cul

ture

Med

ia

Col

iform

s

Bru

cella

Ana

erob

es (I

nclu

ding

Clo

strid

ia)

PRODUCTSAzide Blood Agar Base (AM1006/AM5006)



Egg Yolk Emulsion*(A010)










Columbia Blood Agar Base (AM1029/AM5029)

Brucella Selective Supplement* (AS006)




Corn Meal Agar (AM10301/AM50301)


EMB Agar, Levine (AM1040/5040)


ll

l

l

l

l

l

l

l ll

l

l l

l

l

l



APPLICATIONS

Veterinary Testing

Yeas

t & M

ould

s

Vib

rio

Stre

ptoc

occi

Sta

phyl

ococ

ci

Sal

mon

ella

/Shi

gella

Pse

udom

onas

Myc

obac

teriu

m

List

eria

Gen

eral

Pur

pose

Cul

ture

Med

ia

Col

iform

s

Bru

cella

Ana

erob

es (I

nclu

ding

Clo

strid

ia)

PRODUCTSListeria Identification Agar Base PALCAM (AM1055/AM5055)





Liver Infusion Agar (AM10551/AM50551)

Liver Infusion Broth (AM10552/AM50552)

Lowenstein Jensen Medium Base (AM1057/AM5057)

MacConkey Agar Base (AM1058/AM5058)














l

l

l l

l

l

l

l

l

l

l

l

l l l

l

l

l

l

l

l

l l



APPLICATIONS

Veterinary Testing

Yeas

t & M

ould

s

Vib

rio

Stre

ptoc

occi

Sta

phyl

ococ

ci

Sal

mon

ella

/Shi

gella

Pse

udom

onas

Myc

obac

teriu

m

List

eria

Gen

eral

Pur

pose

Cul

ture

Med

ia

Col

iform

s

Bru

cella

Ana

erob

es (I

nclu

ding

Clo

strid

ia)

PRODUCTSSoyabean Casein Digest Agar (AM1091/AM5091)






Tryptose Agar (AM11041/AM51041)


Urea 40%* (AS028)







l

l

l

l

l

l l

l

l l

l

l

l

l

l

0* - Store between 2-8 C.



Products for Agriculture

Ashby's Glucose Agar-- Nitrogen fixing bacteria

Ashby's Mannitol Agar-- Nitrogen fixing bacteria

Czpek Dox Agar

Cetrimide agar base

Cetrimide Broth

Jensen Broth

Jensen Medium

Pseudomonas Agar (For Pyocyanin)

Pseudomonas Agar (For Fluorescein)

Yeast Mannitol agar with 1.5% Agar

Yeast Mannitol Agar with Congo Red

Yeast Mannitol Broth

Pikovskaya's agar

Pikovskaya's Broth

l

l

l

l

l

l

l

l

l

l

l

l

l lll

l lll


PRODUCTS

APPLICATIONS

Yeas

t & M

ould

s

Pen

icill

ium

not

atum

Pse

udom

onas

Rhi

zobi

um

Asp

ergi

llus

nige

r

Azo

toba

cter



Products for Brewery and Fermentation Analysis

APPLICATIONS

Aga

r Med

ia

Liqu

id M

edia

Col

iform

s

Lact

obac

illi

Fermentation Process


Lactobacillus MRS Agar (AM1051/AM5051)

MacConkey Agar with Crystal Violet, NaCl and 0.15%Bile Salts (AM1059/AM5059)



WL Differential Agar (AM1109/AM5109)

WL Nutrient Broth (AM1110/AM5110)

Wort Agar (AM1111/AM5111)


l

l

l

l

l

l

l l

l l

l

Brewery Analysis

PRODUCTS




Products for Medical and

Research Institutes

APPLICATIONS

PRODUCTS

Yers

inia

ent

eroc

oliti

ca

Yeas

t & M

ould

s

Vib

rio

Stre

ptoc

occu

s

Sta

phyl

ococ

cus

Shi

gella

Sal

mon

ella

Pro

teus

Pse

udom

onas

Nei

sser

ia

Myc

opla

sma

Myc

obac

teria

List

eria

Legi

onel

la

Kle

bsie

lla

Hae

mop

hilis

E. c

oli

Cor

yneb

acte

rium

Clo

strid

ia

Bru

cella

Bor

dete

lla

Bac

tero

ides

0C - Cultivation, E - Enrichment, I - Identification, N - Non-Selective and S - Selective Isolation. * - Store between 2-8 C.

Anaerobic Agar (AM1000/AM5000)


Bacteroides Bile Esculin Agar (AM1010/AM5010)

Bacteroides Selective Supplement* (AS001)








Bordet Gengou Agar Base (AM1015/AM5015)

Bordetella Selective Supplement* (AS004)


Briliiant Green Agar Modified (AM1018/AM5018)


Brilliant Green Bile Agar (AM1019/AM5019)




Cetrimide Broth (AM1023/AM5023)

Chloramphenicol Yeast Glucose Agar (AM1024/AM5024)

CLED Agar with Andrade indicator (AM1026/AM5026)

CLED Agar with Bromothymol Blue (AM1027/AM5027)

Coagulase Mannitol Agar Base (AM1028/AM50258)

C

C C

C/I/S

S

S

C/N C/N

C/S

E

S

N

E

S

C

S

N N N N N N N

N N N N N N N

S

S S SColumbia Blood Agar Base (AM1029/AM5029)

Cam

pylo

bact

er

Sel

ectiv

e Is

olat

ion




Research Institutes

APPLICATIONS

PRODUCTS

Yers

inia

ent

eroc

oliti

ca

Yeas

t & M

ould

s

Vib

rio

Stre

ptoc

occu

s

Sta

phyl

ococ

cus

Shi

gella

Sal

mon

ella

Pro

teus

Pse

udom

onas

Nei

sser

ia

Myc

opla

sma

Myc

obac

teria

List

eria

Legi

onel

la

Kle

bsie

lla

Hae

mop

hilis

E. c

oli

Cor

yneb

acte

rium

Clo

strid

ia

Bru

cella

Bor

dete

lla

Bac

tero

ides


Cam

pylo

bact

er

Sel

ectiv

e Is

olat

ion

Brucella Selective Supplement* (AS006)

Campylobacter Selective Supplement with Hemin (Karmali) Modified* (AS007)




Deoxycholate Agar (AM10303/AM50303)




EMB Agar (AM10391/AM50391)


EM Broth (AM10401/AM50401)






G.C Agar Base (AM1046/AM5046)

G.C Supplement* (AS012)

Haemoglobin Solution* (AS014)

Hektoen Enteric Agar (AM104821/AM504821)



Legionella Agar Base (AM1054/AM5054)

Legionella Growth Supplement* (AS016)

E/C

S S

S S

C

S

C/S C/S

N N N

C/S C/S

N N N

E E E

E

E E

C

C/S C

S S

I I I I I

E

S




Research Institutes

APPLICATIONS

PRODUCTS

Yers

inia

ent

eroc

oliti

ca

Yeas

t & M

ould

s

Vib

rio

Stre

ptoc

occu

s

Sta

phyl

ococ

cus

Shi

gella

Sal

mon

ella

Pro

teus

Pse

udom

onas

Nei

sser

ia

Myc

opla

sma

Myc

obac

teria

List

eria

Legi

onel

la

Kle

bsie

lla

Hae

mop

hilis

E. c

oli

Cor

yneb

acte

rium

Clo

strid

ia

Bru

cella

Bor

dete

lla

Bac

tero

ides


Cam

pylo

bact

er

Sel

ectiv

e Is

olat

ion

Legionella Selective Supplement* (AS017)

Listeria Identification Agar Base,PALCAM (AM1055/AM5055) S

S

C/N

C/N

N N N N N N

N N N N N

N

I I

C

C

C

S

I

I I I

C/S

C/N


Listeria Oxford Medium Base (AM105512/AM505512)

Loeffler Medium Base (AM1056/AM5056)

Horse Serum* (AS015)

Lowenstein Jensen Medium Base (AM1057/AM5057)

Gruft Mycobacterial Supplement* (AS013)

Mac Conkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts(AM1059/AM5059)

MacConkey Agar without Crystal Violet, NaCl and with 0.5% Sodium Tauracholate (AM1061/AM5061)


Malonate Broth Ewing Modified (AM1065/AM5065)





M-FC Agar/Broth Base (AM506923/AM506924)


Mycoplasma Agar Base (PPLO Agar) (AM1073/AM5073)

Mycoplasma Enrichment Supplement* (AS019)

Horse Serum* (AS015)





Research Institutes

APPLICATIONS

PRODUCTS

Yers

inia

ent

eroc

oliti

ca

Yeas

t & M

ould

s

Vib

rio

Stre

ptoc

occu

s

Sta

phyl

ococ

cus

Shi

gella

Sal

mon

ella

Pro

teus

Pse

udom

onas

Nei

sser

ia

Myc

opla

sma

Myc

obac

teria

List

eria

Legi

onel

la

Kle

bsie

lla

Hae

mop

hilis

E. c

oli

Cor

yneb

acte

rium

Clo

strid

ia

Bru

cella

Bor

dete

lla

Bac

tero

ides


Cam

pylo

bact

er

Sel

ectiv

e Is

olat

ion


Preston Agar Base (AM10831/AM50831)




Pseudomonas Isolation Agar (AM1084117/AM5084117)


(AM509312)

(AM11002/AM51002

C/N

S

S

S

C

S

C

C

E

I I I I I

S S

C C C

C C C

S

S S S

S

E E

C C

I I I I

C

I I I I

E

Sabouraud Chloramphenicol Agar (AM1086/AM5086)






Standard Nutrient Agar (AM509311)

Standard Nutrient Broth

Staphylococcus Agar No. 110 (AM10932/AM50932)

Stuart Transport Medium (AM1094/AM5094)



Todd Hewitt Broth (AM10964/AM50964)


Tryptic Digest Broth (AM1100/AM5100)

Tryptone Glucose Beef Extract Agar (TGB Agar)





Research Institutes

APPLICATIONS

PRODUCTS

Yers

inia

ent

eroc

oliti

ca

Yeas

t & M

ould

s

Vib

rio

Stre

ptoc

occu

s

Sta

phyl

ococ

cus

Shi

gella

Sal

mon

ella

Pro

teus

Pse

udom

onas

Nei

sser

ia

Myc

opla

sma

Myc

obac

teria

List

eria

Legi

onel

la

Kle

bsie

lla

Hae

mop

hilis

E. c

oli

Cor

yneb

acte

rium

Clo

strid

ia

Bru

cella

Bor

dete

lla

Bac

tero

ides


Cam

pylo

bact

er

Sel

ectiv

e Is

olat

ion



Urea 40%* (AS028)


Urea 40%* (AS028)







Yersinia Selective Agar Base (AM1116/AM5116)


I

I I I I

I I I I

N

S

S S

C/N

C/N

C/S



Products for Biochemical Tests

Ure

ase

Test

Met

hyl R

ed T

est

Mal

onat

e U

tiliz

atio

n

Leci

thin

ase

Test

Indo

le P

rodu

ctio

n

Hyd

roge

n S

ulph

ide

Pro

duct

ion

Hae

mol

ysis

Tes

t

Esc

ulin

Hyd

roly

sis

Citr

ate

Util

izat

ion

Car

bon

Util

izat

ion

Med

ia W

ith M

ore

Than

O

ne C

arbo

hydr

ate

Med

ia W

ith O

ne

Car

bohy

drat

e

Med

ia W

ithou

tC

arbo

hydr

ates

APPLICATIONS

PRODUCTS

Carbohydrate Fermentation

Deo

xyrib

onuc

leas

e Te

st

Andrade Peptone Water(AM1001/AM5001)

Baird Parker Agar Base(AM1011/AM5011)








Bromothymol Blue Lactose Agar (AM1021/AM5021)

Christensen Citrate Agar(AM1025/AM5025)

Columbia Blood Agar Base (AM1029/AM5029)

Decarboxylase agar Base (AM503023)






Malonate Broth, Ewing Modified (AM1065/AM5065)


Motility Test Medium ( Edwards and Ewing) BIS(AM506934)

l

l

l

l

l

l

ll

l

l

l

l l

l l



Products for Biochemical Tests

Ure

ase

Test

Met

hyl R

ed T

est

Mal

onat

e U

tiliz

atio

n

Leci

thin

ase

Test

Indo

le P

rodu

ctio

n

Hyd

roge

n S

ulph

ide

Pro

duct

ion

Hae

mol

ysis

Tes

t

Esc

ulin

Hyd

roly

sis

Citr

ate

Util

izat

ion

Car

bon

Util

izat

ion

Med

ia W

ith M

ore

Than

O

ne C

arbo

hydr

ate

Med

ia W

ith O

ne

Car

bohy

drat

e

Med

ia W

ithou

tC

arbo

hydr

ates

APPLICATIONS

PRODUCTS

Carbohydrate Fermentation


Deo

xyrib

onuc

leas

e Te

st



Nitrate Broth

Peptone Water (AM1079/AM5079)

Peptone Water with Phenol Red (AM1080/AM5080)

ll

l

l

l

l

l l

l

l

l

Phenol Red Dextrose Broth (AM50802)


Phenol Red Mannitol Broth (AM50806)






Urea 40%* (AS028)


Urea 40%* (AS028)



APPLICATIONS

Cosmetic Analysis

PRODUCTSAnaerobic Agar (AM1000/AM5000)






Brilliant Green Bile Broth 2%(AM1020/AM5020)

Bromothymol Blue Lactose Agar (AM1021/5021)



Cetrimide Broth (AM1023/5023)

CLED Agar with Andrade Indicator (AM1026/AM5026)

CLED Agar with Bromothymol Blue (AM1027/AM5027)


Czpek Dox Agar (AM10302/AM50302)







Letheen Agar Modified (AM505411)

Letheen Broth Modified (AM50543)

Letheen Broth (AM50542)

Lysine Medium Base (AM10577/AM50577)

l

l

l

l

l

l

l

l

l

l

l

l

l

l

l

l l

l

l

Yeas

t & M

ould

Cou

nts

Yeas

t & M

ould

s

Ste

rility

Tes

t

Sta

phyl

ococ

ci

Pse

udom

onas

Ent

erob

acte

riace

ae

Bac

illus

Bac

teria

l Cou

nts

Ana

erob

es (i

nclu

ding

C

lost

ridiu

m)

Col

iform

s

Lact

obac

illi



MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts (AM1059/AM5059)

MacConkey Agar without Crystal Violet and with 0.15% Bile Salts(AM1060/AM5060)

Malonate Broth, Ewing Modified (AM1065/AM5065)







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l

l

l

l

l

l

l

l

l

l

l

l

l

l

l

l

ll

l

ll

l l












Pseudomonas Agar (For Pyocyanin) (AM108414/AM508414)

Pseudomonas Isolation Agar Base (AM108417/AM508417)


APPLICATIONS

Cosmetic Analysis

Yeas

t & M

ould

Cou

nts

Yeas

t & M

ould

s

Ste

rility

Tes

t

Sta

phyl

ococ

ci

Pse

udom

onas

Ent

erob

acte

riace

ae

Bac

illus

Bac

teria

l Cou

nts

Ana

erob

es (i

nclu

ding

C

lost

ridiu

m)

Col

iform

s

Lact

obac

illi

PRODUCTS



APPLICATIONS

Cosmetic Analysis

PRODUCTS

Yeas

t & M

ould

Cou

nts

Yeas

t & M

ould

s

Ste

rility

Tes

t

Sta

phyl

ococ

ci

Pse

udom

onas

Ent

erob

acte

riace

ae

Bac

illus

Bac

teria

l Cou

nts

Ana

erob

es (i

nclu

ding

C

lost

ridiu

m)

Standard Nutrient Broth (AM509312)

Staphylococcus Agar No. 110 (AM10932/AM50932)

Staphylococcus Selection Agar (AM50933)


Tryptone Soya Agar with Lecithin and Tween 80 (AM11031/AM51031)




l

l

l

l

ll

l

l


Col

iform

s

Lact

obac

illi



Cetrimide agar Base

Cetrimide Broth

Czpek Dox Agar

Letheen Broth

Letheen Agar Modified

Letheen Broth Modified

Lysine Medium Base

Plate Count Agar`

Pseudomonas Agar (For Pyocyanin)

Pseudomonas Agar (For Fluorescein)

Pseudomonas Isolation Agar Base

Sabouraud Dextrose Agar

Sabouraud Dextrose Broth

Standard Nutrient Broth

Staphylococcus Selection Agar

Staphylococcus Agar No. 110

Malt Extract Broth

Tryptone Soya Agar with Lecithin and Tween 80

l

l

l

l

l

l

l

llll ll

l

l

l

l

l

l ll

l

l

l

ll

APPLICATIONS

Product for Environmental and sanitary testing

PRODUCTS

Yeas

t & M

ould

s

Ste

rility

Tes

t

Sta

phyl

ococ

ci

Pse

udom

onas

Ent

erob

acte

riace

ae

Bac

illus

Col

iform

s

Stre

ptoc

occu

s

Lact

obac

illi

Pic

hia

ferm

enta

ns



APPLICATIONS

Textile, Petroleum and Molecular Biology

PRODUCTS

Col

iform

s

Luria Agar

Luria Broth

]Luria Bertani Agar, Miller

Luria Bertani Broth, Miller

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l

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