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Unusual mesenchymal liver tumors in adults: Ultrasoundfindings with CT, MR and pathologic correlationKim KW, Kim KA, Park M-S, Kim PN, Lee M-G, Ha HK, UlsanUniversity Seoul Asan Medical Center, Korea; YongDong SeveranceHospital, Yonsei University, Korea

Mesenchymal tumors of the liver are defined as benign and malignanttumors with vascular, fibrous, adipose and other mesenchymal tissuedifferentiation and may have variable faces in radiologic concerns,presenting a difficult diagnostic challenge. Although sonographic find-ings of mesenchymal liver tumors are varied in adults and there may beconsiderable overlap between each other and even with more commonmalignant epithelial tumors, however, in some cases, their sonographicappearances may reflect pathologic features. Therefore, being familiarwith sonographic findings of the mesenchymal liver tumors and under-standing their pathological background is important for the differentialdiagnosis of a focal hepatic lesion found on sonography. In this exhibit,we review a broad spectrum of mesenchymal liver tumors in adults andpresent their sonographic characteristics, with pathologic correlation.

3597

Development of novel lipid nanobubbles as a gene deliverycarrierTakizawa T, Suzuki R, Tanaka K, Sawamura K, Negishi Y, HagisawaK, Utoguchi N, Maruyama K, Teikyo University, School ofPharmaceutical Sciences, Japan; Tokyo University of Pharmacy andLife Science, School of Pharmacy, Japan; The National DefenseMedical College, Japan

Objectives: We developed novel lipid nanobubbles that were associ-ated with perfluoropropane known as US imaging gas. Lipidnanobubbles were made from conventional liposomes. In this study, weassessed the feasibility of lipid nanobubbles as a gene delivery carrier.Methods: COS-7 cells were mixed with naked plasmid DNA (pCMV-Luc) and lipid nanobubbles. Then, the mixture was exposed with US.After the cells were washed and cultured for 48 hr, luciferase activitywas measured.Results: Lipid nanobubbles could effectively deliver plasmid DNA tocells by combination with US exposure without cytotoxicity. In addi-tion, lipid nanobubbles combined with US exposure completed genedelivery into the cells for short time extremely. Moreover, lipidnanobubbles could effectively deliver plasmid DNA though it wasexistence of the serum.Conclusions: Lipid nanobubbles might be novel and effective genedelivery carriers. Now, we are attempting to optimize the condition ofUS exposure and lipid components. Also we are studying about in vivogene delivery.

3598

Umbilical venous blood flow velocity in normal fetus with orwithout pulsationMotohisa C, Nishihara R, Yamamasu S, Tachibana D, Tahara M,Nishimoto S, Nishio J, Iwanaga N, Nakai Y, Ishiko O, Osaka CityUniversity, Graduate School of Medicine, Japan; Izumi-OtsuMunicipal Hospital, Japan

Objectives: Some fetuses without any change in their central flowvelocity pattern reveals pulsatile flow pattern in the umbilical vein. Theaim of this study is to clarify the difference of umbilical venous bloodflow velocity in normal fetus with or without pulsation.Methods: Two hundred sixty normal fetuses between 16 to 41 gesta-tional weeks were crossectionally examined by pulsed Doppler method.

Flow velocity waveforms were acquired from free loop of umbilicalvein. Reference value of umbilical venous flow velocity was calculatedfrom the fetuses who revealed constant velocity flow, and the maxi-mum and minimum velocities of the fetuses with pulsatile venous flowwere compared to the standard values.Results: Two hundred twenty fetuses revealed constant velocity flowsand the other 40 revealed pulsatile pattern. In constant flow group,umbilical venous blood flow velocity increased from second to thirdtrimesters. The maximum velocities of pulsatile venous flow werewithin the reference values.Conclusions: The venous blood flow velocity is thought to be main-tained in the pulsatile group of normal fetuses.

3599

Assessment of left atrial function with strain rate imaging inpatients with coronary artery diseaseChu H, Renmin Hospital of Wuhan University, China

Objectives: To evaluate left atrial (LA) local function in patients withcoronary artery disease (CAD) by strain rate imaging.Methods: LA lateral wall and septal wall strain rate, LA internaldiameter, the peak flow velocities during early diastole (E), atrialcontraction (A), E/A ratio, A duration and the peak velocity of rightupper pulmonary venous flow (PVs, PVd and PVa) were determined in28 normal subjects and 32 patients with CAD and without left atrialdilatation by strain rate imaging and traditional echocardiography. Theresults of the two groups were compared.Results: (1) Compared with control group, LA internal diameter, mitralvalve flow pattern and pulmonary venous flow pattern were not signif-icantly different (p � 0.05). (2) LA lateral wall and septal wall strainrate were significantly different between CAD group and control group(p � 0.05).Conclusions: LA local dysfunction is able to be detected by strain rateimaging in patients without other echocardiographic features of cardiacdiastolic function involvement.

3600

Doppler ultrasound appearances of various vascularcomplications following living donor liver transplantation:Review and updateKim SY, Kim KW, Park SH, Lee SS, Shin YM, Kim PN, Lee M-G,Lee SG, Asan Medical Center, University of Ulsan College ofMedicine, Korea

Living donor liver transplantation (LDLT) becomes more commonplace as a result of the shortage of cadaveric livers. LDLT has majoradvantages over transplantation from cadaveric donors in that livingdonors have completely normal liver function with hemodynamic sta-bility and graft preservation time can be markedly reduced, providingwell-preserved graft viability. However, as the graft volume is muchsmaller than that of cadaveric liver, an intensive monitoring of hemo-dynamic status is the key for a successful outcome. Doppler ultrasoundis generally regarded as the primary screening method for this purpose.Familiarity with the sophisticated surgical procedures, the spectrum ofDoppler ultrasound abnormalities and their substantial diagnostic val-ues permit early detection of various vascular complications followingLDLT. In this exhibit, we present a illustrative review of the surgicaltechniques of LDLT and a spectrum of Doppler ultrasound appearancesof various vascular complications. The emphasis is placed on their factsand fallacies.

Abstracts P293