3.6. order disorderfolk.ntnu.no/audunfor/7. semester/biopolymerkjemi... · order ↔ disorder...
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3.6. Order ↔ DisorderIntroductory examples
TBT4135 Biopolymers
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Introductory examples
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Melting of DNA monitored by fluorescence
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Ribonuclease: Heat denaturation monitored by intrinsic viscosity
Differential scanning calorimetry
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Slow (constant heating)Exo/endothermic peak
Chiroptical methods(Polarometry)
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pH driven denaturation of BSA (bovine serum albumin) at different temperatures
Turbidity (transmittance)
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Heat-driven precipitation of HPMC(Hydroxypropyl methyl cellulose)
RheologyGel melting/setting of fish gelatine
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1. Cool from 30C to 4C(melting)
2. Keep at 4C
3. Heat from 4C to 30C(setting)
Other methods
• Circular dichroism (ellipsometry)• Diffusion coefficient• Sedimentation coefficient (ultracentrifuge)• Light scattering (X-ray scattering): RG, A2
• Osmometry (A2)• NMR
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Prion protein
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Gelation/crystallisation of polysaccharides
• Alginates: Chain dimerisation with Ca+
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• ϰ-carrageenan: Gelation with K+
• Agarose: Chain association and gel formation by cooling