International Congress on Applied

Biological SciencesNovember 08-12 I Sarajevo

5TH

w w w . i c a b s . g e n . t r

ICABS 2019

SARAJEVO

Book of Abstracts

1

ICABS 2019

It is my honor to announce successful completion of 5th International Congress on Applied Biological Sciences (ICABS) which was organized in Sarajevo, Bosnia and Herzegovina on November 08-12, 2019.

The fast advancements that we are witnessing in biological sciences are remarkable, which has a positive impact not only on science and technology but also on economic growth and development.

Sarajevo is the capital and largest city of Bosnia and Herzegovina, with a population of 275,524 in its administrative limits. The Sarajevo metropolitan area, including Sarajevo Canton, East Sarajevo and nearby municipalities, is home to 555,210 inhabitants.Nestled within the greater Sarajevo valley of Bosnia, it is surrounded by the Dinaric Alps and situated along the Miljacka River in the heart of the Balkans.

Congress was provided the opportunity to share knowledge and experience in various fields of applied biological sciences while connecting scientists from different disciplines and geographies.

Sincerely,

Assoc. Prof. Dr. Aslı Özkök, Hacettepe University

Chair of the Congress

Prof. Dr. Farhat Jabeen, GC University Faisalabad, Pakistan

President of Organizing Committe

1

INTERNATIONAL SCIENTIFIC COMITTEE

• Prof Dr. Erhan MUTLU, Akdeniz University, Turkey • Prof. Dr. Farhat Jabeen, GC University Faisalabad, Pakistan • Prof. Dr. Serra Hepaksoy, Ege University, Turkey • Prof. Dr. Süleyman KOZAT, Yuzuncu Yıl University, VAN • Prof.Dr Antonis Kokkinakis , Aristotle University , Thessaloniki, Greece • Prof.Dr Dritan Topi Faculty of Natural Sciences , University of Tirana, Albania • Prof.Dr Ilija Brceski , University of Belgrade, Serbia • Prof.Dr Ilirjan Malollari , Academy of Sience of Albania, University of Tirana,

Albania • Prof.Dr Kemajl Kurteshi, Department of biology, University of Pristina, Kosovo • Prof.Dr Suzana Golemi, Dean of Natural Sciences Faculty ,University of Shkodra

"Luigj Gurakuqi", Albania • Prof.Noureddine DJEBLI, Mostaganem University , Algeria • Prof. Dr. Vladimir Pesic, University of Montenegro, Montenegro • Assoc. Prof. Dr. Attila KARSİ, Mississippi State University, USA • Assoc. Prof. Dr. Filiz VARDAR, Marmara University, Turkey • Assoc. Prof. Dr. Zahid Iqbal, Isra University, Islamabad-Pakistan • Assoc. Prof. Dr. Eltjon Halimi , Depatment of Biology, University of Tirana, Albania • Assoc.Prof Hesat Aliu , Tetova University , North Macedonia • Dr. Majlinda SANA, The University of Durres "Aleksander Moisiu", Albania • Assoc.Prof Kimete Lluga, Department of biology, University of Pristina, Kosovo

ORGANISING COMMITTEE

Conference Chairman

• Prof. Dr. Farhat Jabeen, GC University Faisalabad, Pakistan

Chair of Organizing Commitee

• Assoc Prof Dr Aslı OZKOK, Hacettepe University, Turkey

Members of Organizing Commitee

• Prof. Dr. Farhat Jabeen, GC University Faisalabad, Pakistan • Dr.Gafur Xhabiri, University of Tetova, Macedonia • Dr.Gezim Bara, University of Tirana, Albania • Dr.Lorina Lici Polytechnic University, Albania • Dr.Merije Elezi, University of Tetova, Macedonia

1

*Oral Presentation

THE EFFECT OF BERBERINE IN THE PREVENTION AND / OR TREATMENT OF TESTICULAR INJURY DUE TO CYCLOPHOSPHAMIDE IN RATS

MAHMUT ÖZDEMIR1, HAKAN ALTUNTAŞ1, SEMRA YIĞITASLAN1, VAROL ŞAHINTÜRK2

1ESKIŞEHIR OSMANGAZI UNIVERSITY, FACULTY OF MEDICINE, DEPT. OF PHARMACOLOGY

2ESKIŞEHIR OSMANGAZI UNIVERSITY, FACULTY OF MEDICINE, DEPT. OF HISTOLOGY AND EMBRYOLOGY

Abstract

We aimed to investigate the preventive and/or therapeutic effect of berberine on cyclophosphamide-induced testicular injury. After body weight was measured, a total of 40 rats were randomly divided into 5 groups of 8. The animals in the control group received oral SF for 14 days. The rats in the CP, ber75, ber150 and ber300 groups were given orally, SF, 75, 150 and 300 mg / kg berberine for 14 days, respectively, and all animals were injected i.p. 200 mg / kg CP on day 8. 24 hours after the last dose, the animals were weighed again and the testicular tissues were removed under general anesthesia and then euthanized. After weights were measured, testicular tissues were used for histological examination, immunohistochemical apoptosis evaluation and homogenate preparation. Oxidative stress and cytokine levels in testicular homogenate were evaluated. While CP had no significant effect on body and testicular weights, berberine reduced the body weight in a dose-dependent manner. Although there was no significant change in oxidative stress markers and cytokine levels, degenerative changes and apoptotic findings were found in the CP group and these were improved by dose-dependent treatment with berberine. These effects of berberine may be due to their anti-degenerative and anti-apoptotic activity rather than being mediated by oxidative stress and inflammation.

In conclusion, our findings suggest that berberine treatment may be useful in the prevention / treatment of CP-dependent testicular injury.

Keywords: Berberine, Cyclophosphamide, Rat, Testicular Injury

*This study is supported by Scientific Activities Support Program of Eskişehir Osmangazi University

2

*Oral Presentation

EFFECTS OF FENTANYL ON PANCREATIC CANCER CELL PROLIFERATION AND CANCER STEM CELL DIFFERENTIATION

FEYZI CELIK1, TUGCE DURAN2,3

1DEPARTMENT OF ANESTHESIOLOGY, FACULTY OF MEDICINE, DICLE UNIVERSITY, DIYARBAKIR, TURKEY

2DEPARTMENT OF MEDICAL GENETICS, FACULTY OF MEDICINE, KTO KARATAY UNIVERSITY, KONYA, TURKEY

3DEPARTMENT OF MEDICAL GENETICS AND MOLECULAR BIOLOGY, INSTITUTE OF HEALTH SCIENCES, FACULTY OF MEDICINE, KOCAELI UNIVERSITY, KOCAELI, TURKEY

Abstract

Pancreatic cancer is one of the most aggressive cancer due to the late diagnosis and failure to respond to the treatment despite advances in tumor biology and the development of new cancer therapeutic strategies. It has been reported that these characteristics of pancreatic cancer originate from cancer stem cells within the tumor mass. It has also been reported that the Fentanyl and some other mu-opioid receptors are involved. In this study, we determined the effect of Fentanyl on PANC-1 cells, by assessing the gene expression of cancer stem cell marker genes (Nanog, Oct4, and Sox2) and apoptosis-related genes (BAD, Bax, Bcl-2, and p53) by Quantitative RealTime PCR. The number of cancer stem cells was determined by flow cytometry. The results of our study showed that Fentanyl administration decreased the number of cancer and cancer stem cells in the PANC-1 cell population, decreased the gene expression of stem cell marker and increased the expression of apoptosis-related genes. These results indicate that Fentanyl, which is used routinely in the pain palliation of pancreatic cancer, can be considered as an option in the treatment of pancreatic cancer.

Keywords: Apoptosis; Cancer stem cell; Fentanyl; PANC-1 cell

*This project is supported by Dicle University Scientific Research Projects Coordination Office with TIP.18.016 project numbers

3

*Oral Presentation

ANTIFUNGAL AND ANTI-BIOFILM ACTIVITY OF NEW PROPANE SULFONYL HYDRAZONE AGAINST CANDIDA ISOLATES

MERVE AYDIN1, ALI OZTURK2, UMMUHAN OZDEMIR OZMEN3

1DEPARTMENT OF MEDICAL MICROBIOLOGY, FACULTY OF MEDICINE, KTO KARATAY UNIVERSITY, KONYA, TURKEY

2DEPARTMENT OF MEDICAL MICROBIOLOGY, FACULTY OF MEDICINE, NIĞDE ÖMER HALISDEMIR UNIVERSITY, NIĞDE, TURKEY

3DEPARTMENT OF CHEMISTRY, FACULTY OF SCIENCE, GAZI UNIVERSITY, ANKARA, TURKEY

Abstract

Candidiasis is the most frequently encountered fungal disease that ranges from mild mucosal infections to serious candidaemia and disseminated candidiasis. There are only limited classes of antifungal agents available for physicians to combat Candida infections. In addition, biofilm cells display phenotypic traits that resulting in enhanced resistance to antifungal drugs. Therefore, the limited option to combat fungal threat has raised the interest in seeking alternative antifungal compounds. The aim of this study was to investigate the antifungal and anti-biofilm activity of the new Sulfonyl hydrazones compound Anaf-Psh (3-hydroxynaphthalene-2ethylidenepropane sulfonylhydrazone), derived from sulfonamides. The Candida species evaluated in this study included the five reference strains and thirty-five clinical isolates belonging to a collection of fungal strains previously established at the Gazi University Medical Mycology Laboratory. Identification was performed by conventional methods and by biochemical characterization using the API ID32C® system (Biomerieux) and was confirmed by the sequencing. The antifungal activity of Anaf-Psh was determined using a broth microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) standards. The compound concentrations varied between 0.5–256  µg/ml. The anti-biofilm effect of the synthesized compound was evaluated in 96-well polystyrene flat-bottom microplates. The compound was found to be effective against all tested Candida strains with MIC ranging from 8-64 µg/ml and also inhibit biofilm formation in tested isolates. As a conclusion, it's in vitro antifungal and anti-biofilm properties, this new compound Anaf-Psh is a promising new agent for the control and treatment of Candida infections.

Keywords: Anti-Biofilm Activity, Antifungal Activity, Candida, Sulfonyl Hydrazones

*This research received no specific Grant from any funding agency in the public, commercial, or not-for-profit sectors

4

*Oral Presentation

EVALUATION OF NUTRITIONAL HABITS OF ATHLETES BETWEEN 18-40 YEARS

MERVE PEHLİVAN, FATMANUR TERCAN

İSTANBUL MEDIPOL ÜNIVERSITESI

Abstract

Aim: This study aims to investigate dietary habits and the prevalence rate of disordered eating behaviors of 107 athletes (age, 18-40 years), using questionnaire. Material and method: Participants (50 males, 57 females) completed a questionnaire including anthropometric measurements, dietary habits questions and eating attitude test (EAT-40). The analysis of acquired datas was done using SPSS (version 21.0) and significance for statistical datas was selected being p<0.05.

Results: The mean BMI of male athletes was 24.1±2,9 kg/m2, while the mean of female athletes was 21.04±2,8 kg/m2 and both groups were within normal BMI ranges. While 58.9% of the athletes stated that they skipped meals, the most skipped meal was breakfast with 42.9%. It was observed that most athletes consume meat (%94,4) and dairy products (%96,3). 12.3% of female athletes and 14.0% of male athletes consume sports drinks; energy drink consumption for male and female athletes is 24.6% and 26.0%, respectively. Most of the male and female athletes (80.7% and 86.0%, respectively) were not predisposed to eating disorders. There was no significant relationship between athletes' eating attitude test scores and BMI, body weight, type of sport and regular breakfast (p>0,05). Conclusion: Having healthy eating habits is very important for athletes to maximize their performance. Regular education should be given to athletes to gain healthy eating habits and protect them from eating disorders.

Keywords: Dietary Habits, Athlete, Eating Attitude Test

*

5

*Oral Presentation

EFFECT OF CYSTINE AND BORON ON SPERM QUALITY AND MEMBRANE INTEGRITY AFTER FROZEN-THAWED OF PIRLAK RAM SEMEN

FATIH AVDATEK, DENIZ YENİ

AFYON KOCATEPE UNIVERSITY

Abstract

Non-essential means that our bodies produce an amino acid, even if we do not get it from the food we eat. Non-essential amino acids include: alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, proline, serine, and tyrosine. The aim of this study is to investigate the effects of cystein and boron adding to extender on ram sperm motility, membran integrity and viability test (HOST/E), after frozen-thawed of ram semen. Ejaculates were collected from five Pirlak rams using an artificial vagina ones a week and this process was repeated six times. Ejaculates were split into three aliquots and diluted to a final concentration of 150x106 spermatozoa/ml with the base extender containing cystine 1 mM, boron 1 mM and no antioxidant (control). Following dilution, the specimens were drawn in 0.25 ml different color aliquots and subjected to 3 hours of equilibration at 5 ºC, followed by freezing in 15 minutes in nitrogen vapor (~ -110 ºC), the specimens were stored frozen in liquid nitrogen (-196 ºC) until in vitro evaluation. After being stored for six months, the aliquots were individually thawed in a water bath at 37 ° C for 20 s for microscopic evaluation.

Sperm motility was increased, abnormal sperm rate were decreased significantly (p<0.05) in boron, and membrane integrity were increased significantly (p < 0.05) in cystine when compared to the control group.

İn conclusion boron and cystine improve spermatological parmeters and membrane integrity in breeding season.

Keywords: Boron, Cystine, Membrane Integrity, Ram Sperm

*

6

*Oral Presentation

EFFECT OF DIFFERENT DURATION OF INTRAVAGINAL SPONGE TREATMENTS ON THE SOME REPRODUCTIVE PARAMATERS IN THE

SAANEN GOATS

MUHAMMED KÜRŞAD BIRDANE

AFYON KOCATEPE UNIVERSITY

Abstract

The aim of this study was to investigate the effect of different duration treatments of progesterone sponges on the some reproductive parameters in the Saanen goats at weather conditions of Afyonkarahisar/Turkey. Before the study, ultrasonography examination was performed for reproductive disorders and total 68 multiparaus and lactating Saanen goats were selected from herd. In July, intravaginal sponges (Chronogest®) were applicated in all of groups within the same day for estrus synchronization. Sponges were removed on the days 7th,9th,11th,13th in the groups 1, 2, 3 ,4 respectively (in each group n=17). All group were injected with 480 lU PMSG (Chronogest-PMSG® i.m) and 125 µg Cloprostenol (Estrumate® i.m) one day before sponge remove day. At 24 hours after the removal of the sponges, three male goats were placed in each groups for natural mating. Pregnancy was checked through transrectal ultrasound in dorsal recumbency position on days 25-30 after sponge remove day. The estrus and pregnancy rates were found 82% (14/17), 88% (15/17), 82% (14/17), 81% (13/16) and 57% (8/14), 73%(11/15), 64%(9/14), 53%(7/13) in the groups 1,2,3,4 respectively. Litter size were found 1,87(15/8), 1,90(21/11), 2,0(18/9) and 1,71(12/7) in the groups. Reproductive parameters were statistically insignificant between groups. As a result of this study, the synchronization of estrus with this method did not important change on the reproductive performance in the Saanen goats.

Keywords: Flugestone Acetate, Lactating Goats, Pregnancy Rate, Afyonkarahisar,

*

7

*Oral Presentation

POPULAR DIET PRACTICES AND KNOWLEDGE LEVELS OF ADULT INDIVIDUALS AGED BETWEEN 18-65 YEARS

FATMA ELIF SEZER, BURCU USTAD, MUSTAFA SAFA YONTEM

ISTANBUL MEDIPOL UNIVERSITY, FACULTY OF HEALTH SCIENCES, DEPARTMENT OF NUTRITION AND DIETETICS

Abstract

This study aimed to determine the popular diet practices and knowledge levels of adult individuals aged between 18-65 years. The study was conducted with 300 participants (150 males, 150 females) in Istanbul. The participants' level of knowledge about popular diets and the resources they learned were obtained through a pre-prepared questionnaire. To evaluate the data, SPSS 22.0 statistical package program was used. It was found that the average age was 33.2±10.4 years and 50.3% of the participants were normal, 45.7% were overweight and obese. Popular diets followed by 24% of women, 16.7% of men. It was determined that 91.7% of women and 70.8% of men lost weight with these diets. However, 70.3% of women and 68.8% of men regained weight in the first 6 months after quitting diet. The weight lost (7.08±5.09 kg) was found to be similar to the weight gained after quitting diet (7.24±6.7 kg). Participants learned popular diets from internet/social media (25.8%), TV/radio (24.8%) and their close circle (24.4%). The most well-known popular diets; Karatay diet (24.2%), sugar free diet (14.7%) and gluten-free diet (12.5%). Popular diets are effective in losing weight in the short term, but do not contribute to weight management by causing weight gain again in the long term. Incorrect nutrition and diet practices in weight management should be prevented. Adequate, balanced, healthy nutrition should be provided with the support of dietician. The negative effects of popular diets should be explained by health professionals and the community should be raised awareness.

Keywords: Weight Management, Popular Diet, Malnutrition, Weight Loss

*

8

*Oral Presentation

UNUSUAL LOCATION OF SPIROCHAETES IN LYMPH VESSELS IN BOVINE DIGITAL DERMATITIS LESIONS

MEHMET FATIH BOZKURT, İBRAHIM DEMİRKAN

AFYON KOCATEPE UNIVERSITY, FACULTY OF VETERINARY MEDICINE

Abstract

The precise aetiology of Digital dermatitis has not been established. However, the rapid spread of disease within affected herds and a good respond to topical antibacterial suggest that it may have an infectious cause. Treponemes have been identified in vivo in the lesion and frequently isolated from the lesions and subsequently cultured in vitro. The role of treponemes in its aetiology still remains uncertain. Fourty biopsies were taken from dairy cow suffering from digital dermatitis. They placed in 10% buffered neutral formalin solution for histopathological and spirochaetal immunohistochemical analysis. In immunohistochemical examination, spirochaetal bacteria were detected in 38 samples. It was observed that these bacteria entered between the keratinocytes and reached to the pars papillary dermis. They continued to proliferation and stimulate the inflammatory reaction here. it was found to progress to the deep dermis using lymphatics. Thus, it was observed that they formed a large and severe inflammatory reaction in the dermis. As a result; It was seen that the agents reaching the dermis region closest to the epidermis surface by using the epithelial cells that started to be keratinized from the superficial layer travelled to deeper dermis regions by using lymph vessels. Thus, it was concluded that a more potent inflammatory reaction and its involvement in circulation could lead to more complicated events such as septicemia, affect the clinical course and severity of the disease, and especially localization within lymphatic vessels could be an important mechanism for etiopathogenesis.

Keywords: Digital Dermatitis, Treponema, Immunohistochemistry, Cattle,

*This study is supported by the BAPK of Afyon Kocatepe University with the project no 17.kariyer.211

9

*Oral Presentation

THE EFFECTS OF BENTAGRAM ON THE DEVELOPMENT AND ANTIOXIDANT PARAMETERS OF ARTHROSPIRA PLATENSIS GOMONT

ŞÜKRÜYE ER

SAKARYA UNIVERSITY

Abstract

Herbicides are chemical mixtures used to prevent, control or reduce harmful organisms. These chemicals especially affect the primary producers that constitute the most important link of the food chain affects the whole ecosystem. In this context, the impact of such chemicals on algae is extremely important. In this study, in the C. vulgaris algae exposed to Bentagram, the decrease in OD560 value was observed, while the increase in the amount of chlorophyll-a was significant for 7 days. There was no significant change on SOD, APX and GR enzyme activities. An increase in GR enzyme activity was observed only at a concentration of 960 μg mL-1. MDA and H2O2 amounts are one of the main by-products of lipid peroxidation, which is associated with oxidative stress in cells. When the total MDA amounts of C. vulgaris cultures were compared due to increasing concentrations of the Bentagram, a statistically significant decrease was observed in the concentration of only 960 μg mL-1 compared to the control. The total amount of H2O2 did not show statistically significant change compared to the control. The antioxidant enzyme that determines the accumulation of H2O2 in cells and plays an important role is APX (Asada, 1992). The amount of free proline decreased significantly at 960 μg mL-1, which is the highest concentration compared to the control. Increased GR enzyme activity at the same concentration also supports this information.

As a result, changes in biomass and antioxidant parameters were found to be related to increasing concentrations of Bentagram.

Keywords: Bentagram, Antioxidant Enzyme, Herbicide, Arthrospira Platensis

*This study was supported by Sakarya University Scientific Research Projects (BAP) Commission Presidency (Project No: 2018-02-09-

10

*Oral Presentation

SILENCING OF EAG1 MAY EFFECTS PROLIFERATION AND APOPTOSIS THROUGH GENE EXPRESSION OF ERK1 AND JNK IN CANCER

AHU SOYOCAK1, DIDEM TURGUT COSAN2

1ISTANBUL AYDIN UNIVERSITY MEDICAL FACULTY DEPARMENT OF MEDICAL BIOLOGY, ISTANBUL, TURKEY

2ESKISEHIR OSMANGAZI UNIVERSITY MEDICAL FACULTY DEPARMENT OF MEDICAL BIOLOGY, ESKISEHIR, TURKEY

Abstract

Objective: Extracellular signal-regulated protein kinase (ERK) and c-jun amino-terminal kinase (JNK) are members of the mitogen-activated protein (MAP) kinase family, ERK is activated by growth factors to stimulate cell growth, differentiation, and proliferation, while JNK is activated by stress-induced stimuli and mediates apoptotic signals. It is known that voltage-gated ion channels also play an important role in these cellular processes. Eag1 (KCNH1, Kv10.1, Ether à go-go), a member of the voltage-gated potassium channel family has been shown to have oncogenic features and is overexpressed in many human tumors, including breast cancer. This study aimed to determine the effect of siRNA-mediated silencing of the Eag1 potassium channel on ERK1 and JNK gene expression in strong metastatic MDA-MB-231 cells.

Materials-Methods: ERK1 and JNK gene expressions in Eag1siRNA transfected MDA-MB-231cells were determined on the Real-Time PCR (qRT-PCR (Stratagene, Mx3000p). Gene expression levels were calculated using the formula 2−ΔCt = 2−(Ct gen − Ct housekeeping gen).

Results: It was determined that ERK1 expression levels decreased by -1.75 fold and JNK expression level increases 1.56 fold in Eag1 siRNA transfected MDA-MB-231 cells.

Conclusion: The silencing of the Eag1 gene may alter the signals of proliferation and cell death. Our results suggest that this gene may be a molecular target in the formation or development of breast cancer and further research is needed.

Keywords: Eag1, MDA-MB-231, ERK1, JNK, Gene Expression.

11

*Poster Presentatiom

HUMAN-WILDLIFE INTERACTIONS IN YOUNG GENERATION AWARENESS/PERCEPTION

ISIDORO DE BORTOLI, FILIPPO FAVILLI

EURAC RESEARCH

Abstract

Conflicts and their resolutions among alpine communities represent the process of intensive communication in local societies, and how conflicts are resolved reflect a Region’s political and social structures. Often conflicts occur at the interface between traditional, primary activities and new developing economic activities that have a less environmentally sustainable approach towards natural resources. Nowadays the strain on sharing resources accelerates the tip-over point towards conflicts. We noted that the presence, return and overabundance of adaptable species in the Alpine arch, has found many stakeholders and administrators unprepared. Human presence and expansion in Alpine territories inflict pressure on mobile wildlife species, putting them in the middle of growing conflicts with humans, often hidden from right view of young generation.

Therefore, the educative process is needed where people wrongly understand wildlife and human activities-related impact because of a lack of knowledge. However, such approaches are hindered by the family background of young generation, strongly influenced by household aptitude to wildlife acceptance.

Our analysis identified different human-wildlife interactions in the Alps. The aim of the study is to educate young generation to be aware of main human-wildlife interactions in order to prevent any conflict and to provide them as much as possible right information. To meet these challenges, we propose an abstracted and youthful model that adopts a social-environmental approach to show results have been achieved in interviewing more than 1000 high school students, coming from different parts of the Alpine arch.

Keywords: Interaction, Wildlife, Young Generation, Cohexistence, Conflict

*

12

*Poster Presentatiom

PHYSICOCHEMICAL PROPERTIES OF STIRRED YOGURT PRODUCED USING WHEY PROTEIN, INULIN AND CREAM

EMINE MINE ÇOMAK GÖÇER, FIRUZE ERGİN AHMET KÜÇÜKÇETİN

AKDENİZ ÜNİVERSİTESİ

Abstract

Yogurt is among the most common fermented dairy products consumed all over the world. The standardization of yogurt milk can affect the physicochemical properties of the yogurt which are important acceptance criteria for consumers. In this study, the aim was to investigate the effect of adding whey protein, inulin and cream to yogurt milk on the physicochemical characteristics, including the graininess and roughness, of stirred yogurt. Yogurt milk was standardized by using whey protein, inulin or cream to obtain %15 total solids. The standardized milk was heated at 72 °C for 15 sec and then, cooled to 42 °C. Cooled milk was inoculated with yogurt starter culture. After inoculation, the milk was incubated at 42 °C until the pH decreased to 4.60. At the end of incubation, yogurt samples were manually stirred and stored at 4 °C. The physicochemical properties of the stirred yogurt samples were analyzed on day 1 of storage. The results showed that the highest viscosity, hardness, visual roughness, number of grains and perimeter of grains values were obtained in the stirred yogurt samples produced from standardized milk using whey protein. However, the lowest viscosity, hardness, visual roughness, number of grains and perimeter of grains values were determined in the stirred yogurt samples produced from standardized milk using cream. No significant differences (P>0.05) were found in the total dry matter, pH and titratable values among the stirred yogurt samples produced using whey protein, inulin and cream.

Keywords: Whey Protein, Inulin, Cream, Stirred Yogurt, Visual Roughness, Graininess

*

13

*Oral Presentatiom

COMPARISON OF NATURAL AND CULTURAL FORMS OF VINCA MINOR L. (APOCYNACEAE) SPECIES GROWING IN TURKEY IN TERMS OF

PHARMACEUTICAL BOTANY

ÜMMEHAN GÜNDÜZ, SEVIM KÜÇÜK

ANADOLU UNIVERSITY

Abstract

Vinca L. is most taxa hosting genus of the family Apocynaceae in Turkey. The natural and cultural forms of Vinca minor L. species of this genus were evaluated by comparing their morphological and anatomical features.

V. minor leaves are used in antipyretic, appetizing, constipation, diuretic dermatitis, diuretic, coronal disease, tonsillitis, diarrhea, anemia, hemostatic, bronchitis, gonorrhea, low blood pressure, bleeding arrest, treatment of tumors, oral ulcers and inflammations, hypertension, heart disease and female gynecological problems.

In morphological studies; stem, leaves and flowers were measured and their characters were examined. While flower and leaf color were similar, stem length of the specimens in the natural form was measured as 19-65 cm and culture forms as 10-51 cm. While the number of leaves in natural forms was between 7-20, it was observed that it was between 7-34 in culture forms.

In anatomical studies; root and stem cross-section, leaf cross-section, upper and lower superficial sections were examined. As a result of the anatomical studies, while absorbing hairs were observed in the root of the natural samples, these structures were not found in the culture form samples. In addition, anomocytic stoma was found on the upper surface of the leaf in the culture form, but not in the natural forms.

Keywords: Apocynaceae, Vinca Minor L. Anatomy, Morphology, Turkey

*This study is supported by Scientific Research Projects Program of Anadolu University

14

*Poster Presentatiom

NAFION–GRAPHENE COMPOSITE FILM MODIFIED GLASSY CARBON ELECTRODE FOR VOLTAMMETRIC DETERMINATION OF DOPAMINE

ASIYE ASLIHAN AVAN

ISTANBUL UNIVERSITY-CERRAHPAŞA

Abstract

A Nafion–graphene (Nafion–GR) nanocomposite film modified glassy carbon electrode was fabricated by a simple drop_casting method, and used in the electrochemical detection of dopamine(DA). Owing to the large surface area, good conductivity of GR and good affinity of Nafion, the sensor exhibited excellent electrocatalytic activity for the oxidation of DA. The electrochemical behaviors of DA on Nafion/GR film modified glassy carbon electrodes were investigated by cyclic voltammetry and differential pulse voltammetry. A calibration curve is constructed in the same matrix, urine, as the unknown samples to be analyzed. The Nafion–GR film modified electrode was linearly dependent on the DA concentration and the linear analytical curve was obtained in the ranges of 0.5–200 μM with differential pulse voltammetry (DPV) and the detection limit was 0.051 μM. The Nafion_graphene nanocomposite modified electrode exhibited good reusability than pure graphene-modified GCE. This procedure can be used for the determination of DA in the presence of UA, AA, and TRP. Finally, the proposed method was successfully used to determine DA in urine samples and pharmaceutical preparations.

Keywords: Dopamine, Voltammetry, Graphene, Urine Analysis, Pharmaceutical Analysis

*Istanbul University-Cerrahpaşa

15

*Poster Presentatiom

EVALUATION OF OSTEOPOROSIS KNOWLEDGE MEASUREMENT LEVEL AND NUTRITION IN WOMEN

AYDAN CANKARA, FATMANUR OZYUREK

ISTANBUL MEDIPOL UNIVERSTY

Abstract

Osteoporosis is the most common disease of bone metabolism, which effects individual independence, physical, mental and social well-being and results in morbidity, mortality. The aim of this study was to measure the knowledge levels of osteoporosis in adults with evaluating purpose of nutritional status. The study was planned descriptive cross-sectional research, consisted of 165 adult female volunteers in Istanbul on February-April 2019. The information of the women was gathered face to face interview method with questionnaire. The questionnaire includes the Osteoporosis Knowledge Test, 3-day food consumption record. The data of the dietary records was evaluated with BeBis program. All statistical analyses were analysed with SPSS. The mean age of women was 38.8 ± 14 years and BMI was normal with 50.9%. Osteoporosis Knowledge Test’s mean score of all participants was 19.16 ± 4.02. A very weak and significant negative correlation was observed between age and Osteoporosis Knowledge Test scores. (rho=-0,165*, p=0.034) The daily amount of protein taken by women is 58.77±62.53 g, vitamin C is 68.67±49.56 mg, calcium is 537.90±212,72 mg and phosphorus 849,04±260,33 mg. It was observed that vitamin C and calcium mineral were lower than recommended in all age groups of women. Trainings should be planned to increase the knowledge of osteoporosis. Beginning from young adulthood, people should be given the habit of eating a diet that has a positive effect on bone health and is protective against osteoporosis.

Keywords: Osteoporosis Knowledge, Women, Nutiriton, Calcium

*

16

*Poster Presentatiom

THE EFFECT OF EXAM ANXIETY AND BODY IMAGE ON EATING BEHAVIOR IN ADOLESCENTS

HANDE OTAKLI1, FATMANUR ÖZYÜREK1, NESLİHAN SÜRMELİ2

1MEDİPOL ÜNİVERSİTESİ

2YÜZÜNCÜ YIL ÜNİVERSİTESİ

Abstract

During the adolescent period, healthy nutrition provides the nutrients necessary for the physical, cognitive growth and development of individuals and reduces the risk of feeding-related diseases at the beginning of adulthood. Throughout life, despite the change in body perception, especially adolescence is a period in which body perception and nutrition problems are experienced. In this age group, the desire to reach the ideal body size determined by the society, changes on the diet and wrong diet practices may cause inadequate and unbalanced nutrition. This study was conducted in order to examine the effects of exam anxiety and body perception on feeding behaviors in adolescents. 203 female and 97 male, in total 300 students whose ages from 17 to 19 participated in the study. General information, exam anxiety, body perceptions and eating behaviors of the participants were measured using a questionnaire prepared in advance. Test Anxiety Scale, Netherlands Eating Behavior Survey, Body Appreciation Scale have been applied to the adolescents. When exam anxiety of the students was examined, physical reaction were found to be higher in girls, mental reaction were higher in boys (p<0,05). It was found that girls received significantly higher scores than boys in restrictive eating and emotional eating categories when the participants examined their eating behavior (p<0,05). It was found that obese individuals scored the lowest in restrictive eating behavior and the highest in emotional eating behavior (p<0,05). It has been observed that there is positive and significant relation between restrictive eating behaviour and body liking scores (p=0,00, r=0,201). Healthy eating habits are very important for protecting adolescents from the stress of education, reducing the effects of body perception and coping with stress.

Keywords: Adolescent, Body Perception, Exam Anxiety, Nutrition Behavior

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International Congress on Applied

Biological SciencesNovember 08-12 I Sarajevo

5TH