460-737

APPLICATIONS

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GC Applications by Column Phase . . . . . . . . . . . . .462–463

EPA Method Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .463

HPLC Applications . . . . . . . . . . . . . . . . . . . . . . . . .464-527

Biochemical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .465-469Nucleosides, Nucleotides, Nucleic Acid Bases, Aromatic Amino Acids,Carboxylic Acids

Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .470-488Aldehydes & Ketones, Herbicides, Aromatics, Explosives, Pesticides,Phenols, Phthalate Esters, Polynuclear Aromatic Hydrocarbons

General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .488-496Test Mixes

Dietary Supplements / Plant Extracts . . . . . . . . . . .497-500Extracts, Vitamins

Foods & Flavors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .501-508Antimicrobial Agents, Carboxylic Acids, Sugars, Seasonings, Flavorings

Pharmaceutical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .509-527Analgesics, Antiarrhythmics, Antiasthmatics, Antibiotics, Antidepressants,Anti-Diabetics, Anti-Inflammatories, Anti-Ulceratives, Bronchodilators,Corticosteroids, Steroid Hormones, Mixed Drugs, Sedatives, Xanthenes

GC Applications . . . . . . . . . . . . . . . . . . . . . . . . . . .528-737

Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .529-623Volatiles, Semivolatiles, Pesticides, Herbicides, PCBs, ChlorinatedDisinfection Byproducts, Dioxins, Endocrine Disruptors, Explosives,Gasoline Range Organics (GRO), Diesel Range Organics (DRO), Glycols,Haloacetic Acids, Air Analysis

Foods, Flavors, & Fragrances . . . . . . . . . . . . . . . . . . . .624-653Chiral Separations, Fatty Acids, FAMEs, Essential Oils, Flavors & Fragrances,Food Contaminants, Sterols, Triglycerides, Sugars/Sugar Alcohols

Forensics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .654-660Blood Alcohol, Drugs of Abuse, Anesthetics, Solvents, Inhalants, ArsonAccelerants

Personal Care & Cleaning Products . . . . . . . . . . . . .661-662Solvents, Fragrances

Petroleum & Petrochemicals . . . . . . . . . . . . . . . . . . .663-689Permanent Gases, Fluorocarbon Gases, Hydrocarbon Gases,Hydrocarbons, Sulfur Compounds

Pharmaceutical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .690-704Antidepressants, Antihistamines, Antiepileptics, Barbiturates, Basic Drugs,Chiral Analyses, Pharmaceutical Solvents, Steroids, SympathomimeticAmines

Solvents & Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . .705-737Solvents, Alcohols, Aldehydes, Acrylates, Amines, Aromatics,Chlorofluorocarbons, Cresylic Acids, Esters, Glycols, Ketones, Silanes,Styrene, Siloxanes

Compound Index . . . . . . . . . . . . . . . . . . . . . . . . . .738-745


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Phone: 800-356-1688 or 814-353-1300www.restek.com

Rtx®-1air samples . . . . . . . . . . . . . . . . . . . . 619, 621, 622, 623aromatics (gasoline) . . . . . . . . . . . . . . . . . . . . . . . . 673chlorofluorocarbons. . . . . . . . . . . . . . . . . 730, 731, 732essential oils . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 642fatty acids (free). . . . . . . . . . . . . . . . . . . . . . . . . . . . 630flavor volatiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 643fragrances . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 647, 662hydrocarbons (accelerants) . . . . . . . . . . . . . . . 659, 660hydrocarbons (C7-C42) . . . . . . . . . . . . . . . . . . . . . 678hydrocarbons (crude oil). . . . . . . . . . . . . . . . . . . . . 681hydrocarbons (detailed reformate analysis) . . . . . . 680hydrocarbons (gases) . . . . . . . . . . . . . . . . . . . . 671, 672hydrocarbons (waxes) . . . . . . . . . . . . . . . . . . . 682, 683organophosphorus pesticides . . . . . . . . . . . . . . . . . 582oxygenates . . . . . . . . . . . . . . . . . . . . . . . . . . . . 674, 675ozone precursors . . . . . . . . . . . . . . . . . . . . . . . . . . . 619solvents . . . . . . . . . . . . . . . . . . . 705, 706, 708, 711, 717solvents (pharmaceutical) . . . . . . . . . . . . . . . . 699, 715sulfur compounds . . . . . . . . . . . . . . . . . . . . . . . . . . 688Rtx®-1PONAhydrocarbons (detailed gasoline analysis) . . . . . . . 679MXT®-1HT Sim Disthydrocarbons (crude oil). . . . . . . . . . . . . . . . . . . . . 682hydrocarbons (high temp.) . . . . . . . . . . . . . . . 684, 685MXT®-1 Sim Disthydrocarbons (high temp.) . . . . . . . . . . . . . . . . . . . 685MXT®-500 Sim Disthydrocarbons (high temp.) . . . . . . . . . . . . . . . . . . . 685Rtx®-2887simulated distillation . . . . . . . . . . . . . . . . . . . . . . . . 686Rtx®-XLBorganochlorine pesticides . . . . . . . . . . . . . . . . 574, 575PCBs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 598Rtx®-5 / MXT®-5alcohols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 721benzidines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 546chlorinated disinfection byproducts . . . . . . . . . . . . 599chlorinated hydrocarbons . . . . . . . . . . . . . . . . . . . . 553chlorophenoxyacid herbicides. . . . . . . . . . . . . . . . . 587Diesel range organics (DRO). . . . . . . . . . 612, 613, 614drugs of abuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 656endocrine disruptors . . . . . . . . . . . . . . . . . . . . . . . . 601essential oils . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 639gasoline range organics (GRO) . . . . . . . . 606, 607, 611haloacetic acids . . . . . . . . . . . . . . . . . . . . . . . . . . . . 617haloethers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 552hazardous substances . . . . . . . . . . . . . . . . . . . . . . . 553hydrocarbons (accelerants) . . . . . . . . . . . . . . . . . . . 659nitrosamines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 548nitroaromatics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 548nitrogen herbicides . . . . . . . . . . . . . . . . . . . . . . . . . 590nitrogen/phosphorus pesticides & herbicides. . . . . 586organochlorine pesticides . . . . . . . . . . . . 568, 569, 577PCBs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 592, 593pharmaceuticals (basic) . . . . . . . . . . . . . . 694, 695, 704phenols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 545, 546polynuclar aromatic hydrocarbons . . . . . 550, 551, 552phthalate esters . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547siloxane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 737solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 712steroids. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 701sterols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 651XTI®-5glycols / alcohols . . . . . . . . . . . . . . . . . . . . . . . . . . . 735phenols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 546sterols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 651Rtx®-5MSendocrine disruptors . . . . . . . . . . . . . . . . . . . . . . . . 601semivolatiles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 555volatiles (food packaging) . . . . . . . . . . . . . . . . . . . . 650

Rtx®-5Sil MSaromatics (GRO) . . . . . . . . . . . . . . . . . . . . . . . . . . . 604polynuclear aromatic hydrocarbons . . . . . . . . . . . . 549phenols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 554semivolatiles . . . . . . . . . . . . . . . . . . . . . . . 554, 556-565 Rtx®-5 Amineamines / phenols . . . . . . . . . . . . . . . . . . . . . . . . . . . 725ethanolamines / ethyleneamines . . . . . . . . . . . . . . . 724pharmaceuticals (basic) . . . . . . . . . . . . . . 690, 696, 703Rtx®-20antioxidants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 624essential oils . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 641pharmaceuticals (acid/neutral) . . . . . . . . . . . . . . . . 691Rtx®-1301flavor volatiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 645solvents (pharmacutical) . . . . . . . . . . . . . . . . . 699, 701Rtx®-1701acrylates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 723aldehydes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 723fragrances. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 647organochlorine pesticides . . . . . . . . . . . . . . . . . . . . 577organophosphorus pesticides . . . . . . . . . . . . . . . . . 580pharmaceuticals (acid/neutral) . . . . . . . . . . . . 691, 692pharmaceuticals (basic). . . . . . . . . . . . . . . . . . . . . . 690solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . 710, 717, 718styrene . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 736Rtx®-200aromatics (BTEX) . . . . . . . . . . . . . . . . . . . . . . . . . . 676chlorofluorocarbons . . . . . . . . . . . . . . . . . . . . 731, 732chlorinated disinfection byproducts . . . . . . . . . . . . 599chlorinated hydrocarbons . . . . . . . . . . . . . . . . . . . . 552explosives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 602fatty acids (free). . . . . . . . . . . . . . . . . . . . . . . . . . . . 630glycols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 615, 735haloacetic acids . . . . . . . . . . . . . . . . . . . . . . . . . . . . 617nitrosamines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 548pharmaceuticals (basic) . . . . . . . . . . . . . . 693, 695, 703phenols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 545silanes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 736siloxane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 737solvents . . . . . . . . . . . . . . . . . . . . . . . 705, 707, 708, 710solvents (pharmaceutical) . . . . . . . . . . . . . . . . . . . . 716triazine herbicides . . . . . . . . . . . . . . . . . . . . . . . . . . 588Rtx®-35chlorinated hydrocarbons . . . . . . . . . . . . . . . . . . . . 553chlorophenoxyacid herbicides. . . . . . . . . . . . . . . . . 587endocrine disruptors . . . . . . . . . . . . . . . . . . . . . . . . 601nitrogen herbicides . . . . . . . . . . . . . . . . . . . . . . . . . 590organochlorine pesticides . . . . . . . . . . . . . . . . . . . . 576organophosphorus pesticides . . . . . . . . . . . . . . . . . 580pharmaceuticals (acid/neutral) . . . . . . . . . . . . 692, 693pharmaceuticals (basic) . . . . . . . . . . . . . . . . . . 694, 696Rtx®-35 Amineamines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 736pharmaceuticals (basic) . . . . . . . . . . . . . . . . . . 702, 703Rtx®-50antioxidants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 624chlorophenoxy herbicides . . . . . . . . . . . . . . . . . . . . 589organochlorine pesticides . . . . . . . . . . . . . . . . . . . . 577phenols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 545phthalate esters . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547triazine herbicides . . . . . . . . . . . . . . . . . . . . . . . . . . 588Rtx®-65TGtriglycerides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 652Rtx®-225sugars . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 653Rtx®-440organochlorine pesticides . . . . . . . . . . . . . . . . . . . . 569polynuclear aromatic hydrocarbons . . . . . . . . . . . . 551

Stabilwax®acrylamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 649alcohols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 720alcohols / aldehydes . . . . . . . . . . . . . . . . . . . . . . . . . 722aldehydes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 722amines / alcohols / chlorides . . . . . . . . . . . . . . . . . . 727aromatics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 728, 729essential oils . . . . . . . . . . . . . . . . . . . . . . . 639, 641, 642esters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 733ethers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 734FAMEs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 635flavor volatiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 643glycols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 616hydrocarbons (synthetic lubricant) . . . . . . . . . . . . 682ketones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 735nitrosamines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 548oxygenates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 675solvents . . . . . . . . . . . . . . . 706, 707, 709, 711, 713, 718styrene . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 736Rtx®-WAX / MXT®-WAXaldehydes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 723aromatics (BTEX) . . . . . . . . . . . . . . . . . . . . . . . . . . 676essential oils . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 640FAMEs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 635FAMEs (cis/trans) . . . . . . . . . . . . . . . . . . . . . . . . . . 634glycols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 615solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . 661, 712, 713FAMEWAX™FAMEs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 636, 638RT-CW20M™ F&Fflavors / fragrances. . . . . . . . . . . . . . . . . . . . . . . . . . 648Stabilwax®-DAcresylic acids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 732fatty acids (free) . . . . . . . . . . . . . . . . . . . . . . . . 630, 631flavor volatiles . . . . . . . . . . . . . . . . . . . . . . . . . 644, 646solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 713Stabilwax®-DBamines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 725, 727Rtx®-2330Dioxins (TCDD) . . . . . . . . . . . . . . . . . . . . . . . . . . . 600FAMEs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 635, 637sugars . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 652Rt-2560FAMEs (cis/trans) . . . . . . . . . . . . . . . . . . 632, 633, 634Stx™-CLPesticides/Stx™-CLPesticides2organochlorine pesticides . . . . . . . . . . . . . . . . . . . . 572Rtx®-CLPesticides/Rtx®-CLPesticides2chlorophenoxyacid herbicides. . . . . . . . . . . . . . . . . 589EDB / DBCP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 566haloacetic acids . . . . . . . . . . . . . . . . . . . . . . . . . . . . 618nitrogen/phosphorus herbicides . . . . . . . . . . . . . . . 585organochlorine pesticides 567, 570, 571, 573, 576, 583,584organophosphorus pesticides . . . . . . . . . . . . . . . . . 581polynuclear aromatic hydrocarbons . . . . . . . . . . . . 549PCBs . . . . . . . . . . . . . . . . . . . . . . . . . 594, 595, 596, 597Rtx®-OPPesticides/Rtx®-OPPesticides2organophosphorus pesticides . . . . . . . . . . . . . 578, 579triazine herbicides . . . . . . . . . . . . . . . . . . . . . . . . . . 591Rtx®-VMSsolvents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 661, 662volatiles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 537-543 Rtx®-VGCaromatics (BTEX) . . . . . . . . . . . . . . . . . . . . . . . . . . 608oxygenates . . . . . . . . . . . . . . . . . . . . . . . . . . . . 608, 610volatiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 530, 532Rtx®-VRXvolatiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 531, 536

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GC Applications by Phase, EPA Methods Chart 463


Rtx®-502.2air toxins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 620alcohols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 719alcohols / aldehydes . . . . . . . . . . . . . . . . . . . . . . . . . 721aromatics . . . . . . . . . . . . . . . . . . . . . . . . . 676, 728, 729aromatics (BTEX) . . . . . . . . . . . . . . . . . . . . . . . . . . 609aromatics / aliphatics (GRO). . . . . . . . . . . . . . 605, 607ethers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 734volatiles . . . . . . . . . . . . . . . . . . . . . . . 533, 534, 535, 544Rtx®-Volatilesalcohols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 721solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 713Rtx®-TNT/Rtx®-TNT2explosives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 603Rtx®-BAC1/Rtx®-BAC2anesthetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 657blood alcohol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 654glycols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 616inhalants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 658solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 657Rtx®-G27 & Rtx®-G43solvents (pharmaceutical) . . . . . . . . . . . . . . . . . . . . 700Rt-TCEP™aromatics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 677oxygenates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 674Chiral Columnsflavors / fragrances . . . . . . . . . . . . . . . . . . . . . . 625-629pharmaceuticals . . . . . . . . . . . . . . . . . . . . . . . . 697, 698di-isodecyl phthalate cresylic acids . . . . . . . . . . . . . 733

Micropacked & PLOTHayeSep® Shydrocarbon gases . . . . . . . . . . . . . . . . . . . . . . . . . . 671Rt-Alumina™ PLOThydrocarbon gases . . . . . . . . . . . . . . . . . . 670, 671, 672Rt-Msieve™ 13X PLOThydrocarbon gases . . . . . . . . . . . . . . . . . . . . . . . . . . 666permanent gases. . . . . . . . . . . . . . . . . . . . . . . . 663, 664Rt-Msieve™ 5A PLOTpermanent gases. . . . . . . . . . . . . . . . . . . . . . . . 664, 665Rt-QPLOT™alcohols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 720fatty acids (free). . . . . . . . . . . . . . . . . . . . . . . . . . . . 630hydrocarbon gases . . . . . . . . . . . . . . 666, 667, 668, 669permanent gases. . . . . . . . . . . . . . . . . . . . . . . . 664, 665solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 714Rt-SPLOT™hydrocarbon gases . . . . . . . . . . . . . . . . . . . . . . . . . . 669Rt-UPLOT™hydrocarbon gases . . . . . . . . . . . . . . . . . . . . . . 666, 669solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 714Rt-XLSulfur™sulfurs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 689sulfurs in beverages . . . . . . . . . . . . . . . . . . . . . . . . . 649Rt-Sulfur™sulfurs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 689

ShinCarbon STfluorocarbons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 666natural gas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 668permanent gases . . . . . . . . . . . . . . . . . . . . . . . . . . . 663sulfur dioxide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 688TCEPoxygenates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 674PackedCarboBlack™ Bflavor volatiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6455% Carbowax® 20Mblood alcohol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 655HayeSep Qsolvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7145% Krytox®/CarboBlack™ Bchlorofluorocarbons . . . . . . . . . . . . . . . . . . . . . . . . 7313% Rt-101sugars . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 653sugar alcohols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6535% Rt-1200/1.75% Bentone® 34aromatics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 677Rtx®-1 Sim Dist 2887simulated distillation . . . . . . . . . . . . . . . . . . . . 686, 68710% TCEParomatics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67820% TCEParomatics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 674

GC Applications by Phase (cont.)

EPA 500 Series MethodsMethod # Method Title ..................................................................Page #

502.2 Volatile Organic Compounds ..............................................534504.1 1,2-dibromoethane & 1,2-dibromo-3-chloropropane ........566

508 Chlorinated Pesticides ................................................................567, 568515.1 Chlorophenoxyacid Herbicides ............................................589

524.1/.2 Volatile Organics....................................................................543, 544525 Semivolatile Organics............................................................563526 Semivolatile Organics (screening) ........................................554528 Phenols ..................................................................................554

551.1 Chlorinated Disinfection Byproducts ..................................599552.2 Haloacetic Acids ....................................................................617, 618

E PA 6 0 0 S e r i e s M e t h o d sMethod # Method Title ..................................................................Page #

601 Purgeable Halocarbons ........................................................535602 Purgeable Aromatics..............................................................535604 Phenols ..................................................................................545, 546605 Benzidines ..............................................................................546606 Phthalate Esters......................................................................547607 Nitrosamines..........................................................................548609 Nitroaromatics & Isophorone ..............................................547610 Polynuclear Aromatic Hydrocarbons (HPLC/GC)..............549, 550, 551611 Haloethers ..............................................................................552612 Chlorinated Hydrocarbons ..................................................552, 553615 Chlorophenoxyacid Herbicides ............................................587619 Nitrogen/Phosphorus Herbicides & Pesticides ..................585, 588625 Base/Neutrals, Acids, & Pesticides ........................................564

E PA 8 0 0 0 S e r i e s M e t h o d sMethod # Method Title ..................................................................Page #

8021 Volatile Organics....................................................................530-533, 5368060 Phthalate Esters......................................................................5478081 Chlorinated Pesticides ..........................................................569-5778090 Nitroaromatics & Cyclic Ketones ........................................5478095 Explosives ..............................................................................6038100 Polynuclear Aromatic Hydrocarbons ..................................552

8140/8141/8141A Organophosphorus Pesticides ..............................................578-582

8151 Chlorophenoxyacid Herbicides ............................................5898240 Volatile Organics (8260 short list)........................................539

8260B Volatile Organics....................................................................538, 540-5428270 Semivolatile Organics............................................................556-5608330 Explosives by HPLC ..............................................................474, 4758095 Explosives by GC ..................................................................603

C L P M e t h o d sMethod # Method Title ..................................................................Page #OLC 03.2 Volatile Organics....................................................................537, 562

CLP Semivolatile Organics............................................................555Appendix

IX Semivolatile Organics ....................................................................561Skinner List Semivolatile Organics ....................................................................565

EPA Methods Chart

HPLC & GC Applications

Compound Indexstarting on page 738

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unsatisfactory results. The following pages include HPLC chromatograms for a wide range of samples, including environmental

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Larry PetersHPLC Manufacturing Technician

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465Biochemical


PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. ATP 2582. ADP 3203. AMP 2744. adenine 845. adenosine 254

Adenine, Adenosine, ATP, ADP, and AMP on Ultra IBD

1

2

3

4

5

0 2 4 6 8 10 12 14 16 18 min.LC_0129

SSaammppllee::Inj.: 20µLSolvent: 20mM ammonium

acetate, pH 5.8

CCoolluummnn:: UUllttrraa IIBBDDCat. #: 9175565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: 20mM ammonium

acetate, pH 5.8:methanol (97.5:2.5)

Flow: 1.0mL/minTemp.: 35°CDet.: UV @ 260nm

Nucleotides, Nucleosides, and Nitrogenous Bases on Ultra IBD

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. CDP 1462. CMP 1133. ATP 1174. ADP 1455. cytidine 1556. TMP 1577. AMP 1258. guanine 369. guanosine 103

10. adenine 3811. adenosine 115

0 2 4 6 8 10 12 14 16 18 min.

6 7

89

10

11

12

3

4

5

LC_0128




Flow: 1.0mL/min.Temp.: 35°CDet.: UV @ 260nm


acetate, pH 5.8

Uracil, Uridine, UTP, UDP, and UMP on Ultra IBD

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. UTP 3602. UDP 3603. UMP 2844. uracil 905. uridine 230

SSaammppllee::Inj.: 20µLConc.: 90-360µg/mL

each componentSolvent: 20mM ammonium

acetate, pH 5.8




Flow: 1.0mL/min.Temp.: 35°C Det.: UV @ 260nm

LC_0133

0 2 4 6 min.

1

2

3

4

5

Cytosine, Cytidine, CTP, CDP, and CMP on Ultra IBD

LC_0130

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))::1. CTP 2482. CDP 3223. CMP 2484. cytosine 1005. cytidine 340


acetate, pH 5.8



acetate,pH 5.8:methanol (97.5:2.5)


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466 Biochemical


Guanine, Guanosine, GTP, GDP, and GMP on Ultra IBD

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. GTP 2122. GDP 2603. GMP 3624. guanine 805. guanosine 226


acetate, pH 5.8


CCoonnddiittiioonnss::Mobile phase: 20mM ammonium acetate,

pH 5.8:methanol (97.5:2.5)Flow: 1.0mL/min.Temp.: 35°C Det.: UV @ 260nm

LC_0131

0 2.5 5 7.5 10 12.5 15 min.

1

2

3

4

5

Thymine, Thymidine, TTP, TDP,and TMP on Ultra IBD

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. TTP 2242. TDP 1523. TMP 3464. thymine 885. thymidine 318


acetate, pH 5.8





LC_01320 2 4 6 8 10 12 14 16 min.

1

2

3

45

0 1 2 3 4 5 6 7 8 9 min.

LC_0137

SSaammppllee::Inj.: 1.0µLConc. 100µg/mL eachSolvent: 20 mM ammonium acetate,

pH 5.0 Note: 1-3 drops of ammonium hydroxide added to keep guanine in solution

CCoolluummnn:: AAlllluurree™ PPFFPP PPrrooppyyllCat. #: 9169565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 60Å


acetate, pH 5.0: methanol (85:15, v/v)

Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 260nm

Nucleic Acid Bases on Allure™ PFP Propyl

1

2

3

4

5

PPeeaakk LLiisstt::1. cytosine 2. uracil3. guanine4. thymine5. adenine


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467Biochemical


0 5 10 15 20 25 min.

Purines/Pyrimidines on Ultra PFP

CCoolluummnn:: UUllttrraa PPFFPPCat. #: 9176575Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: 0.001% formic acid in water:

methanol (50:50, v/v) Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 260nm

1

2

3

4

5

PPeeaakk LLiisstt::1. cytosine 2. 5-methylcytosine3. uracil4. uric acid5. hypoxanthine6. xanthine7. thymine8. purine9. theobromine

10. ethyluracil11. 6-methylpurine12. 1,7-dimethylxanthine13. theophylline14. propyluracil

SSaammppllee::Inj.: 10µLConc.: 100mg/mL eachSolvent: mobile phase

6

7

8

11

9 10

121413

LC_0136

LC_0307

Aromatic Amino Acids on Ultra Aqueous C18 (Fast Analysis)

CCoonncc..PPeeaakk LLiisstt:: ((mmgg//mmLL))

1. tyrosine 1.252. phenylalanine 0.553. tryptophan 0.035

SSaammppllee::Inj.: 10µLDiluent: 50mM potassium

phosphate, pH 2.5

CCoolluummnn:: UUllttrraa AAqquueeoouuss CC1188Cat. #: 9178535 Dimensions: 30 x 4.0mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: A: 50mM potassium

phosphate, pH 2.5B: acetonitrile

TTiimmee %%BB((mmiinn..))0.0 01.0 401.2 05.0 0


PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. tyrosine 0.22. phenylalanine 1.63. tryptophan 0.4

SSaammppllee:: Inj.: 20µLSolvent: mobile phase A

Aromatic Amino Acids on Ultra Aqueous C18

CCoolluummnn:: UUllttrraa AAqquueeoouuss CC1188Cat. #: 9178565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phases: A: 50mM potassium


5% - 20% B: 0-5 min.20% - 5% B: 5-6 min.Hold at 5% B: 6-13 min.


0 2 4 6 8 10 min.LC_0146

1

2

3

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468 Biochemical


Carboxylic Acids on Ultra Aqueous C18


CCoonnddiittiioonnss::Mobile phase: 50mM potassium

phosphate, pH 2.5Flow: 1.0mL/min.Temp.: 25°C (or ambient)Det.: UV @ 210nm

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. glycolic acid 5.42. malonic acid 4.23. acetic acid 7.84. maleic acid 0.060

SSaammppllee::Inj.: 10µLSolvent: 50mM potassium

phosphate, pH 2.5

LC_0168


SSaammppllee::Inj.: 10µLSolvent: mobile phase

Carboxylic Acids in 100% Aqueous Mobile Phase on Ultra Aqueous C18



phosphate, pH 2.5Flow rate: 1.0mL/min.Temp.: 25°CDet.: UV @ 210nm

0 1 2 3 4 5 6 7 min.

1

2

3

4

AA.. iinniittiiaallBB.. aafftteerr 33 DDaayyss

LC_0142


PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))::1. malonic acid 5002. lactic acid 5003. acetic acid 10004. citric acid 10005. succinic acid 20006. fumaric acid 10

SSaammppllee::Inj.: 10µLSolvent: water



phosphate, pH 2.5: acetonitrile (99:1)


LC_0140

1

2

3

4

5

6

0 1 2 3 4 min.




phosphate, pH 2.5Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 210nm

LC_0228


1. glycolic acid 2.02. malonic acid 1.83. lactic acid 3.34. acetic acid 2.1


Stable retention times, even with100% aqueous mobile phase!

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Biochemical


LC_0306

Carboxylic Acids on Ultra Aqueous C18(Fast Analysis)

CCoonncc..PPeeaakk LLiisstt:: (mg/ml)

1. glycolic acid 2.62. malonic acid 2.83. acetic acid 4.94. maleic acid 0.0125

SSaammppllee::Inj.: 10µLDiluent: 50mM potassium

phosphate, pH 2.5

CCoolluummnn:: UUllttrraa AAqquueeoouuss CC1188 FFaasstt LLCC CCaarrttrriiddggee ((9911778833334400)) aanndd hhoollddeerr ((2255229988))

Dimensions: 30 x 4.0mmParticle size: 5µmPore size: 100Å







LC_0229

Carboxylic Acids on Ultra IBD

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. glycolic acid 2.02. lactic acid 3.33. acetic acid 2.14. malonic acid 1.8

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Environmental


Aldehydes and Ketones (DNPH derivatives) on Ultra C18(40°C)

0 2 4 6 8 10 12 14 min.

LC_0036

1

2

4

5

7

8

3

6

9

10 11

12 13

PPeeaakk LLiisstt::DNPH derivatives of:

1. formaldehyde2. acetaldehyde3. acetone4. acrolein5. propionaldehyde6. crotonaldehyde7. MEK8. methacrolein9. butyraldehyde

10. benzaldehyde11. valeraldehyde12. m-tolualdehyde13. hexanaldehyde

SSaammppllee::Inj.: 10µLConc.: 3.0µg/mL each, as derivative

CCoolluummnn:: UUllttrraa CC1188Cat. #: 9174565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase:

Solvent A: waterSolvent B: acetonitrileSolvent C: tetrahydrofuran50-30% A/30-65% B/20-5% C, 0-15 min.

Flow rate: 1.5mL/min.Wavelength: 365nmTemp.: 40°C

0 2 4 6 8 10 12 14 16 min.

2

1

3

54

67

8

10 11

12 13

9

Aldehydes and Ketones (DNPH derivatives) on Ultra C18(60°C)

PPeeaakk LLiissttDNPH derivatives of:

1. formaldehyde2. acetaldehyde3. acrolein4. acetone5. propionaldehyde6. crotonaldehyde7. methacrolein8. butyraldehyde9. methyl ethyl ketone (MEK)

10. benzaldehyde11. valeraldehyde12. m-tolualdehyde13. hexanaldehyde

LC_0052

SSaammppllee::Inj. 10µLConc. 3µg/mL as DNPH derivative

CCoolluummnn:: UUllttrraa CC1188Catalog #: 9174565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100ÅCCoonnddiittiioonnss::Mobile phase: Solvent A: water

Solvent B: methanolMode : gradient70-76% B: 0-18 min.


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471Environmental


LC_0298

Aldehydes and Ketones (DNPH derivatives) on Allure™ C18 (15°C)

PPeeaakk LLiisstt:: RReett.. ttiimmee ((mmiinn..))DNPH derivatives of:

1. formaldehyde 3.032. acetaldehyde 3.803. acrolein 4.774. acetone 4.935. propionaldehyde 5.296. crotonaldehyde 6.507. n-butyraldehyde 7.308. benzaldehyde 8.249. isovaleraldehyde 9.72

10. valeraldehyde 10.4011. o-tolualdehyde 11.4012. m-tolualdehyde 11.8313. p-tolualdehyde 12.3314. hexaldehyde 15.2815. dimethylbenzaldehyde 16.43

SSaammppllee::Inj.: 5µL Aldehydes/Ketones DNPH Standard (Cat.# 31808)Conc.: 15µg/mL each componentSolvent: acetonitrile

CCoolluummnn:: AAlllluurree™ CC1188Cat. #: 9164565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 60Å

CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (30:70 v/v)Flow: 1.0mL/min.Temp.: 15°C Det.: UV @ 365nmPressure: 85 bar

0 2

2

1

4 6 8 10 12 14 min.

3

54

6

8

7

9 1011 12 13 14

15

PPeeaakk LLiisstt:: RReett.. TTiimmee ((mmiinn..))DNPH derivatives of:

1. formaldehyde 2.84 2 acetaldehyde 3.533. acrolein 4.354. acetone 4.535. propionaldehyde 4.856. crotonaldehyde 5.817. butyraldehyde 6.638. benzaldehyde 7.249. isovaleraldehyde 8.77

10. valeraldehyde 9.3311. o-tolualdehyde 9.8512. m-tolualdehyde 10.2013. p-tolualdehyde 10.5014. hexanaldehyde 13.5015. dimethylbenzaldehyde 13.95

LC_0294

Aldehydes and Ketones (DNPH derivatives) on Allure™ C18 (25°C)

SSaammppllee:: Inj.: 10µL Aldehydes/Ketones DNPH Standard

(cat.# 31808)Conc.: 15µg/mL eachSolvent: acetonitrile

CCoolluummnn:: AAlllluurree™ CC1188Cat.# 9164565 Dimensions: 150 x 4.6 mmParticle size: 5µmPore size: 60Å

CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (30:70 v/v)Flow: 1.0mL/min.Temp.: 25°C Det.: UV @ 365 nm

EPA TO-11A and 8315EPA TO-11A and 8315

EPA TO-11A and 8315EPA TO-11A and 8315

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LC_0319

Herbicides (Phenylurea) on Ultra C18

PPeeaakk LLiisstt:: CCoonncc.. (µg/mL or ppm)1. tebuthiuron 22. thidiazuron 23. monuron 54. fluometuron 25. diuron 26. propanil 27. siduron 28. linuron 29. carbazole 5

10. diflubenzuron 2

SSaammppllee::Inj.: 20µLConc.: see peak listSample diluent: acetonitrile


CCoonnddiittiioonnss::Mobile phase: A: 25mM phosphate buffer, pH 2.5

B: acetonitrileTTiimmee ((mmiinn..)) %%BB

0 408 4014 5518 5519 40

Flow: 1.5 mL/min.Temp.: ambientDet.: UV @ 245nm

LC_0320

Phenylurea Herbicides on Ultra Cyano

PPeeaakk LLiisstt:: CCoonncc.. (µg/mL or ppm)1. tebuthiuron 22. monuron 53. thidiazuron 24. fluometuron 25. diuron 26. propanil 27. siduron 28. linuron 29. carbazole 5

10. diflubenzuron 2

CCoolluummnn:: UUllttrraa CCyyaannooCat. #: 9106575Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile Phase: A: 25mM phosphate buffer, pH 2.5

B: acetonitrileTime (min.) %B

0 308 3014 5021 50

21.1 30Flow: 1.5mL/minTemp.: ambientDet.: UV @ 245nm


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0 2 4 6 8 10 12 14 min.

1

2

3

4

5

CCoolluummnn:: PPiinnnnaaccllee DDBB CC1188Cat. #: 9414565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 140Å

CCoonnddiittiioonnss::Mobile phase: water:methanol

(20:80)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

LC_0256

Aromatics on Pinnacle™ DB C18

PPeeaakk LLiisstt:: CCoonncc..1. propylbenzene 15mg/mL2. butylbenzene 15mg/mL3. o-terphenyl 0.2mg/mL4. amylbenzene 15mg/mL5. triphenylene 0.1mg/mL

SSaammppllee:: Inj.: 5µLSolvent: methanol

0 2

2

1

3

4

5

4 6 8 10 12 14 16

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. propylbenzene 150002. butylbenzene 150003. o-terphenyl 2004. amylbenzene 150005. triphenylene 100

SSaammppllee::Inj.: 5µL Solvent: methanol

CCoolluummnn:: PPiinnnnaaccllee II™ CC1188Cat. #: 9214565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: water:methanol (20:80 v/v)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

LC_0258

Aromatics on Pinnacle II™ C18

BTEX Mix on Pinnacle II™ C18CCoolluummnn:: PPiinnnnaaccllee II™ CC1188Catalog #: 9214565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: water:acetonitrile

(50:50, v/v)Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 254nm

PPeeaakk LLiisstt::1. benzene2. toluene3. o-xylene4. ethylbenzene5. m-xylene & p-xylene

SSaammppllee::Inj.: 10µLConc.: 200µg/mL eachSolvent: methanol

LC_0161


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PPeeaakk LLiisstt::1. HMX2. RDX3. 1,3,5-trinitrobenzene4. 1,3-dinitrobenzene5. tetryl6. nitrobenzene7. 2,4,6-trinitrotoluene8. 2-amino-4,6-dinitrotoluene9. 4-amino-2,6-dinitrotoluene

10. 2,6-dinitrotoluene11. 2,4-dinitrotoluene12. 2-nitrotoluene13. 4-nitrotoluene14. 3-nitrotoluene

SSaammppllee::Inj.: 3µL*Conc.: 500µg/mL

each componentSolvent: acetonitrile

*Equal amounts of 8330 Calibration Mix #1 (cat.# 31450)8330 Calibration Mix #2 (cat.# 31451)

CCoolluummnn:: PPiinnnnaaccllee II™ CC1188 Cat. #: 9214575Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 110Å


(50:50, v/v) Flow: 1.5 mL/min.Temp.: 27°C Det.: UV @ 254nm

Explosives by EPA Method 8330 on Pinnacle II™ C18

LC_0189

0 2 4 6 8 10 12 14 16 18 min.

16

1514

13

119

1210

6

8

7

5

4

3

21

LC_0254

Explosives by EPA Method 8330 on Ultra C18

PPeeaakk LLiisstt::1. HMX2. RDX3. 1,2-dinitrobenzene (SS)4. 1,3,5-trinitrobenzene5. 1,3-dinitrobenzene6. tetryl7. nitrobenzene8. 3,4-dinitrobenzene (IS)9. 2,4,6-trinitrotoluene

10. 4-amino-2,6-dinitrotoluene11. 2-amino-4,6-dinitrotoluene12. 2,6-dinitrotoluene13. 2,4-dinitrotoluene14. 2-nitrotoluene15. 4-nitrotoluene16. 3-nitrotoluene

SSaammppllee::Inj.: 5µLConc.: 30µg/mL (30ppm) eachSample diluent: acetonitrile


CCoonnddiittiioonnss::Mobile phase: water:methanol (44:56, v/v)Flow: 1.0mL/min.Temp.: 30°CDet.: UV @ 254nm

PPeeaakk LLiisstt::1. nitrobenzene2. 2-nitrotoluene3. 4-nitrotoluene4. 3-nitrotoluene5. 1,3-dinitrobenzene6. 1,3,5-trinitrobenzene7. 2,6 dinitrotoluene8. 2,4-dinitrotoluene9. 2,4,6-trinitrotoluene

10. 4-amino-2,6-dinitrotoluene11. 2-amino-4,6-dinitrotoluene12. RDX13. tetryl14. HMX

SSaammppllee::Inj.: 3µLConc.: 500µg/mL eachSolvent: acetonitrile

CCoolluummnn:: PPiinnnnaaccllee II™ CCyyaannoo Cat. #: 9216575Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 110Å


(50:50, v/v) Flow: 1.5mL/min.Temp.: 27°C Det.: UV @ 254nm

Explosives by EPA Method 8330 on Pinnacle II™ Cyano

LC_0188

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2, 34

5

6

7

14

23

4

5

6

13

12

11

10

9

8

7

1

8 9 10 min.

PPeeaakk LLiisstt::1. nitrobenzene2. 2-nitrotoluene3. 3-nitrotoluene4. 4-nitrotoluene5. 1,3,5-trinitrotoluene6. 1,3-dinitrobenzene7. 2,6-dinitrotoluene8. 2,4-dinitrotoluene9. 2,4,6-trinitrotoluene

10. 4-amino-2,6-dinitrotoluene11. 2-amino-4,6-dinitrotoluene12. RDX13. tetryl14. HMX

Explosives by EPA Method 8330 on Ultra Cyano

0 10 20 30 40 50 60 min.LC_0109




SSaammppllee::Inj.: 5µLConc.: 100µg/mL eachSolvent: water:methanol (50:50, v/v)

Explosives by EPA Method 8330 on Allure™ C18

2

LC_0055

PPeeaakk LLiisstt::1. HMX2. RDX3. 1,3,5-trinitrobenzene4. 1,3-dinitrobenzene5. nitrobenzene6. tetryl7. 3,4-dinitrotoluene (IS)8. 2,4,6-trinitrotoluene9. 4-amino-dinitrotoluene

10. 2-amino-dinitrotoluene11. 2,6-dinitrotoluene12. 2,4-dinitrotoluene13. 2-nitrotoluene14. 4-nitrotoluene15. 3-nitrotoluene

1

3

4

5

6

7

8

9

10

11

12

13

14

15

CCoolluummnn:: AAlllluurree™ CC1188Cat. #: 9164575Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 60Å


(50:50 v/v)Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 254nm

0 5 10 15 20 25 30 35 40 min.

SSaammppllee::Inj.: 5µLConc.: 100µg/mL eachSolvent: mobile phase

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LC_0314

Herbicides (Phenoxyacid Mix B) on Ultra Aqueous C18

PPeeaakk LLiisstt::1. 4-nitrophenol2. MCPA3. 3,5-dichlorobenzoic acid4. MCPP5. 2,4,5-T6. 2,4-DB7. 1,4-dichlorobenzene (IS)8. dinoseb9. pentachlorophenol

SSaammppllee::Inj.: 10µLConc.: 10 ppm each herbicideSample diluent: acetonitrile


CCoonnddiittiioonnss::Mobile phase: A: 0.05% H3PO4

B: acetonitrile

TTiimmee ((mmiinn..)) %%BB0 10

10 4516 4522 9024 9025 10

Flow: 1.0mL/minTemp.: ambientDet.: UV @ 225nm


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LC_0313

Herbicides (Phenoxyacid Mix A) on Ultra Aqueous C18

PPeeaakk LLiisstt::1. picloram2. chloramben3. dicamba4. bentazon5. 2,4-D6. dichlorprop7. 2,4,5-TP8. acifluorfen9. 1,4-dichlorobenzene (IS)

SSaammppllee::Inj.: 10µLConc.: 10ppm each herbicideSample diluent: acetonitrile


CCoonnddiittiioonnss::Mobile phase: A:0.05% H3PO4

B:acetonitrile


15 8020 8021 20


0 2 4

1

2

3

6 8 10 12 14 min.

SSaammppllee::Inj.: 10µLConc.: 10ppm eachSolvent: 0.05% phosphoric acid : acetonitrile, 50:50


CCoonnddiittiioonnss::Mobile phase: 0.05% phosphoric acid : acetonitrile, 60:40Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 225nm

LC_0304

Herbicides on Ultra Aqueous C18

PPeeaakk LLiisstt::1. 2,4-D2. Silvex (2,4,5-TP)3. 1,4-dichlorobenzene (IS)

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CCoonnddiittiioonnss::Mobile phase: A: water


0 408 40

10 9015 90

Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 240nm

PPeeaakk LLiisstt::1. fenuron2. monuron3. chlorotoluron4. isoproturon5. diuron6. linuron7. neburon

SSaammppllee::Inj.: 10µL uron herbicide mixConc.: 35.7µg/mL eachSolvent: acetonitrile

Herbicides (Uron) on Pinnacle II™ C8

LC_0190

LC_0309

SSaammppllee::Inj.: 5µL custom mixConc.: approx. 20µg/mL eachDiluent: acetonitrile



B: acetonitrile


10 4015 4026 8034 80

34.1 20


PPeeaakk LLiisstt:: RReett.. TTiimmee ((mmiinn..))1. cyanazine 12.042. methabenzthiazuron 13.883. chlortoluron 14.594. secbumeton 15.745. isoproturon 16.186. diuron 16.787. linuron 22.488. flusilazole 24.299. ethofumesate 24.53

10. neburon 25.7411. pendimethalin 31.46

Herbicides on Allure™ C18

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free literatureEEnnvviirroonnmmeennttaall HHPPLLCC:: AApppplliiccaattiioonnss--CCoolluummnnss--RReeffeerreennccee MMaatteerriiaallss((lliitt.. ccaatt..## 5599774411))

Save time, improve separations, using these columnsand conditions.

Restek HPLC columns support environmental HPLCapplications with rapid analysis times and effectiveanalyte resolution. Sample turn-around can be 50%faster, or more, than with alternative columns. In addi-tion, we prepare analytical reference materials andsolid phase extraction products for these methods.Applications described in this 8-page publicationinclude polyaromatic hydrocarbons, carbamates, phe-noxyacid herbicides, explosives, and carbonyls.

Request from your Restek representative, or visit ourwebsite.

PPeeaakk LLiisstt:: RReett.. ttiimmee ((mmiinn..))1. methomyl 3.802. carbendazim 4.933. aldicarb 6.534. chlortoluron 8.655. isoproturon 9.456. diuron 9.907. monolinuron 10.438. linuron 15.36

SSaammppllee::Inj.: 5µLConc.: 500µg/mL eachSolvent: N,N-dimethylacetamide:acetonitrile

(28:72 v/v)

CCoolluummnn:: AAlllluurree CC1188Cat. #: 9164565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 60Å

CCoonnddiittiioonnss::Mobile phase: A: 20mM potassium phosphate, pH 7.0

B: acetonitrile

TTiimmee ((mmiinn..)) %%BB0.0 203.0 4512.0 4513.0 8014.0 2024.0 20

Flow: 1.0mL/min.Temp.: 25°CDet.: UV @ 220nm and 254nm

Herbicides (Uron) on Allure™ C18

LC_0305

254nm

220nm

Pesticide (Diflubenzuron) on Ultra IBD

0 2 4 6 8 10 12 14 min.

PPeeaakk LLiisstt::1. diflubenzuron2. N,N'-bis-(chlorobenzyl)urea

SSaammppllee::Inj.: 10µLConc.: 1mg/mLSolvent: water:acetonitrile

(1:1, v/v)

CCoolluummnn:: UUllttrraa IIBBDDCat. #: 9175365Dimensions: 150 x 4.6mmParticle size: 3µmPore size: 100Å


(45:55, v/v)Solvent A: 65% 0.1M ammonium

acetate pH 6.0Flow rate: 1.0mL/min.Temp.: ambientDet.: UV @ 260nm

1

2

LC_0081

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Pesticides (Carbamates) on Ultra Carbamate

LC_0308

SSaammppllee::Inj.: 10µLConc.: 100µg/mL each component

CCoolluummnn:: UUllttrraa CCaarrbbaammaatteeCat. #: 9177315Dimensions: 100 x 4.6mmParticle size: 3µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: A: 90% water: 10% methanol with

10mM ammonium formateB: 90% acetonitrile: 10% methanol

with 10 mM ammonium formate

TTiimmee ((mmiinn..)) %%BB0.0 10%15 90%20 90%

Flow: 1.0mL/min. (approx. 0.75mL/min. to UV detector, 0.25mL/min. to MSD)

Temp.: ambientDet.: UV @ 220nm; MSD

MMSSDD CCoonnddiittiioonnss::Detector: Micromass ZMDMode: ESI+Capillary V: 3.50Extractor: 4.0Ion energy: 0.4Multiplier: 650Source temp: 100°CDesolv. temp: 250°CGas flow: 490 L/hr.

CCoommppoouunndd IIoonn CCoonnee VV1. aldicarb sulfone 223.3 25V2. aldicarb sulfoxide 207.3 18V3. oxamyl 237.2* 10V4. methomyl 163.2 15V5. 3-hydroxycarbofuran 238.3 15V

*Ammonium adduct (all other are [M+H]+ ions)

CCoommppoouunndd IIoonn CCoonnee VV6. aldicarb 191.2 8V7. propoxur 210.2 18V8. carbofuran 222.3 22V9. carbaryl 202.2 18V

10. methiocarb 226.3 19V

3-hydroxycarbofuran

methiocarb

aldicarb sulfone

carbofuran

propoxur

aldicarb sulfoxide

carbaryl

aldicarb

methomyl

UV @220nm

oxamyl

PPeeaakk LLiisstt::1. aldicarb2. aldicarb sulfoxide3. oxamyl4. methomyl5. 3-hydroxycarbofuran6. aldicarb7. propoxur8. carbofuran9. carbaryl

10. methiocarb11. 4-bromo-3,5-dimethylcarbamate

CCoolluummnn:: UUllttrraa CCaarrbbaammaatteeCat. #: 9177355Dimensions: 50 x 4.6mmParticle size: 3µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: A: 90:10 water:methanol

B: 90:10 methanol:acetonitrile

TTiimmee ((mmiinn..)) %%BB0 1010 90


Pesticides (Carbamates) on Ultra Carbamate (Fast LC)

LC_0225

SSaammppllee::Inj.: 5µL cat. # 32274 and

cat. # 32273 mixed 50:50Conc.: 50µg/mL eachSolvent: methanol

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Pesticides on Allure™ C18

PPeeaakk LLiisstt::1. desisopropyl atrazine2. desethyl atrazine3. simazine4. terbuthylazine desethyl5. cyanazine6. methabenzthiazuron7. chlortoluron8. atrazine9. secbumeton

10. isoproturon11. diuron12. terbumeton13. terbuthylazine14. linuron15. flusilazole16. ethofumerate17. neburon

SSaammppllee::Inj.: 5µLConc.: approx. 10ppm each pesticideSolvent: acetonitrile


CCoonnddiittiioonnss::Mobile phase A: 20mM K2HPO4, pH 7.0

B: acetonitrile


10 4015 4030 70

30.1 20


LC_0310

PPeeaakk LLiisstt::1. desisopropyl atrazine2. desethyl atrazine3. simazine4. terbuthylazine desethyl5. cyanazine6. atrazine7. methabenzthiazuron8. secbumeton9. chlortoluron

10. isoproturon11. terbumeton12. diuron13. terbuthylazine14. linuron15. ethofumerate16. flusilazole17. neburon

SSaammppllee::Inj.: 5µLConc.: approx. 10ppm each pesticideSolvent: acetonitrile

CCoolluummnn:: AAlllluurree™ PPFFPP PPrrooppyyllCat. #: 9169355Dimensions: 50 x 4.6mmParticle size: 3µmPore size: 60Å

CCoonnddiittiioonnss::Mobile phase A: 20mM K2HPO4, pH 7.0

B: acetonitrile


10 3012 3018 5026 50

26.1 20


Pesticides on Allure™ PFP Propyl

LC_0312

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0 2 4

1

2

3

6 8 10 12 14 16 min.

PPeeaakk LLiisstt:: CCoonncc..1. phenol 0.6mg/mL2. 2,6-dinitrophenol 1.4mg/mL3. 3,5-dinitrophenol 1.5mg/mL


CCoolluummnn:: PPiinnnnaaccllee™ DDBB CC1188Cat. #: 9414565Dimensions: 150mm x 4.6mmParticle size: 5µmPore size: 140Å

CCoonnddiittiioonnss::Mobile phase: 0.7% acetic acid:methanol (60:40 v/v)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

LC_0272

Phenols on Pinnacle™ DB C18

LC_0274

Phenols on Pinnacle II™ C18

PPeeaakk LLiisstt:: ccoonncc.. ((mmgg//mmLL))1. phenol 0.62. 2,6-dinitrophenol 1.43. 3,5-dinitrophenol 1.5

SSaammppllee::Inj.: 5µLSolvent: methanol

CCoolluummnn:: PPiinnnnaaccllee IIII™ CC1188Cat. #: 9214565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: 0.7%acetic acid:methanol (60:40 v/v)Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 254nm

PPeeaakk LLiisstt:: PPeeaakk ttiimmee (min.)1. diquat 7.52. paraquat 8.8

CCoolluummnn:: UUllttrraa QQuuaattCat. #: 9181565Dimensions: 150 x 4.6mmParticle size: 5µm

CCoonnddiittiioonnss::Mobile phase: Ultra Quat Reagent Solution (cat.# 32441):

acetonitrile, 95:5 (v/v)Flow: 1.0 mL/min.Temp.: 27°C Det.: UV @ 257nm (paraquat)

UV @ 308nm (diquat)

SSaammppllee::Inj.: 20µLConc.: 20ppm each component (above);

20µg/mL, 40µg/mL, and 100µg/mLeach component at 308nm (left) and at 257nm (right)

Sample diluent: water

Paraquat and Diquat on Ultra Quat Column

min.

12

LC_0321

min.

diquat308nm paraquat

257nm

100µg/mL

40µg/mL

20µg/mL

100µg/mL

40µg/mL

20µg/mL

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0 2 4 6 8 10min.

1

2

34

56

PPeeaakk LLiisstt::1. dimethyl phthalate2. diethyl phthalate3. di-n-butyl phthalate4. butyl benzyl phthalate5. bis(2-ethylhexyl)phthalate6. di-n-octyl phthalate

SSaammppllee::Inj.: 5µL Method 606-phthalate

esters calibration mix (cat.# 31031)

Conc.: 2,000µg/mL each in methanol

CCoolluummnn:: PPiinnnnaaccllee II™ PPhheennyyllCat. #: 9199565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (30:70 v/v)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254 nm

LC_0178

Phthalate Esters on Pinnacle II™ Phenyl(Isocratic Elution)

LC_0180

0 5 10 15 min.

1

2

3 45

6


B: acetonitrileTTiimmee ((mmiinn..)) %% BB

0 605 6010 8015 80


Column and sample information same as forisocratic elution (LC_0178).

Phthalate Esters on Pinnacle II™ Phenyl(Gradient Elution)

LC_0179

0 2.5 5 7.5 10 12.5 15 17.5 20 22.5 min.

1

2

3

4

56

PPeeaakk LLiisstt::1. dimethyl phthalate2. diethyl phthalate3. butyl benzyl phthalate4. di-n-butyl phthalate5. bis(2-ethylhexyl)phthalate6. di-n-octyl phthalate

SSaammppllee::Inj.: 5µL Method 606-phthalate esters

calibration mix (cat.# 31031)Conc.: 2,000µg/mL each

in methanol

CCoolluummnn:: PPiinnnnaaccllee II™ CC88Cat #: 9213565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (20:80 v/v)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

Phthalate Esters on Pinnacle II™ C8(Isocratic Elution)


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0 2 4 6 8 10 12 14 16 18 min.

1

23

4

6

7 8

9

11

13

12

15

16

14

10

5

SSaammppllee::Inj.: 5µLSolvent: 1:9 methylene chloride:acetonitrile

CCoolluummnn:: PPiinnnnaaccllee II™PPAAHHCat. #: 9219563Dimensions: 150 x 3.2mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: A: purified water, B: acetonitrile

TTiimmee ((mmiinn..)) %%BB0 407 6011 100

17.9 10018 40


LC_0303

Polynuclear Aromatic Hydrocarbons on Pinnacle II™ PAH

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. naphthalene 1002. acenaphthylene 1003. acenaphthene 1004. fluorene 1005. phenanthrene 506. anthracene 1007. fluoranthene 508. pyrene 509. benzo(a)anthracene 50

10. chrysene 5011. benzo(b)fluoranthene 5012. benzo(k)fluoranthene 5013. benzo(a)pyrene 5014. dibenzo(a,h)anthracene 5015. benzo(ghi)perylene 5016. indeno(1,2,3-cd)pyrene 50

SSaammppllee::Inj.: 610 PAH Calibration Mix A (cat.# 31264), 5µLSample diluent: methylene chloride:acetonitrile (1:9, v/v)CCoolluummnn::Catalog #: 9219563Dimensions: 150 x 3.2mmParticle size: 5µmPore size: 110ÅCCoonnddiittiioonnss::Mobile phase: A: water; B: acetonitrile

TTiimmee ((mmiinn..)) %%BB0 4030 10040 10041 4051 40

Flow: 0.5mL/minTemp.: 30°C (or ambient)Det.: UV @ 254nm

Reproducibility for Column Lots: EPA Method 610 PAHs on Pinnacle II™ PAH

LC_0243

RReett.. TTiimmee ((mmiinn..)) CCoonncc..PPeeaakk LLiisstt:: LLoott 11 LLoott 22 LLoott 33 ((µgg//mmLL))

1. naphthalene 11.95 12.17 11.80 1002. acenaphthylene 13.41 13.65 13.26 1003. acenaphthene 15.61 15.89 15.45 1004. fluorene 16.09 16.31 15.92 1005. phenanthrene 17.41 17.61 17.23 506. anthracene 18.80 18.92 18.61 1007. fluoranthene 20.23 20.39 20.03 508. pyrene 21.31 21.46 21.11 509. benzo(a)anthracene 24.88 24.87 24.65 50

10. chrysene 25.64 25.54 25.40 5011. benzo(b)fluoranthene 28.62 28.53 28.35 5012. benzo(k)fluoranthene 29.97 29.74 29.70 5013. benzo(a)pyrene 31.26 31.02 30.97 5014. dibenzo(a,h)anthracene 33.09 32.74 32.81 5015. benzo(ghi)perylene 34.46 34.24 34.16 5016. indeno(1,2,3-cd)pyrene 35.19 34.85 34.91 50

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PPeeaakk LLiisstt:: CCoonncc.. (µg/mL or ppm)1. naphthalene 52. acenaphthylene 53. 1-methylnaphthalene 54. 2-methylnaphthalene 55. acenaphthene 56. fluorene 57. phenanthrene 58. anthracene 59. fluoranthene 5

10. pyrene 511. benzo(a)anthracene 512. chrysene 513. benzo(b)fluoranthene 514. benzo(k)fluoranthene 515. benzo(a)pyrene 516. dibenzo(a,h)anthracene 517. benzo(ghi)perylene 518. indeno(1,2,3-cd)pyrene 5

SSaammppllee::Inj.: 5µL cat.# 31841Conc.: see peak listSample diluent: acetonitrile

CCoolluummnn:: PPiinnnnaaccllee IIII™ PPAAHHCat. #: 9219563Dimensions: 150 x 3.2mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: A: purified water


0 407 6011 10017.9 10018 40


Polynuclear Aromatic Hydrocarbons on Pinnacle II™ PAHEPA 8310-18 Component Standard

LC_0317

LC_0318

Polynuclear Aromatic Hydrocarbons on Pinnacle II™ PAHEPA 8310 - 20 Component Standard

PPeeaakk LLiisstt:: CCoonncc.. (µg/mL or ppm)1. naphthalene 1002. acenaphthylene 1003. 1-methylnaphthalene 1004. 2-methylnaphthalene 1005. acenaphthene 1006. fluorene 1007. phenanthrene 508. anthracene 1009. o-terphenyl 100

10. fluoranthene 5011. pyrene 5012. p-terphenyl 5013. benzo(a)anthracene 5014. chrysene 5015. benzo(b)fluoranthene 5016. benzo(k)fluoranthene 5017. benzo(a)pyrene 5018. dibenzo(a,h)anthracene 5019. benzo(ghi)perylene 5020. indeno(1,2,3-cd)pyrene 50



CCoonnddiittiioonnss::Mobile phase: A: purified water


0 407 60

11 10017.9 100

18 40Flow: 1.2mL/min.Temp.: ambientDet.: UV @ 254nm

Great resolution in lessthan 18 minutes!

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PPeeaakk LLiisstt::1. naphthalene2. acenaphthylene3. acenaphthene4. fluorene5. phenanthrene6. anthracene7. fluoranthene8. pyrene9. benzo(a)anthracene

10. chrysene11. benzo(b)fluoranthene12. benzo(k)fluoranthene13. benzo(a)pyrene14. dibenzo(a,h)anthracene15. benzo(ghi)perylene16. indeno(1,2,3-cd)pyrene

SSaammppllee::Inj.: 5µLConc.: 5-10 ppm eachSample diluent: methylene chloride:acetonitrile (1:9 v/v)

CCoolluummnn:: PPiinnnnaaccllee IIII™™ PPAAHHCat. #: 9219555Dimensions: 50 x 4.6mmParticle size: 5µmPore size: 110Å



0 501.5 604.0 1005.5 1005.6 50


Polynuclear Aromatic Hydrocarbons on Pinnacle II™ PAH(Fast LC)

LC_0315

PPeeaakk LLiisstt::1. naphthalene2. acenaphthylene3. 1-methylnaphthalene4. 2-methylnaphthalene5. acenaphthene6. fluorene7. phenanthrene8. anthracene9. o-terphenyl

10. fluoranthene11. pyrene12. p-terphenyl13. benzo(a)anthracene14. chrysene15. benzo(b)fluoranthene16. benzo(k)fluoranthene17. benzo(a)pyrene18. dibenzo(a,h)anthracene19. benzo(ghi)perylene20. indeno(1,2,3-cd)pyrene

SSaammppllee::Inj.: 5µLConc.: 5-10ppm eachSample diluent: methylene chloride:acetonitrile (1:9 v/v)




0 501.5 604.0 1005.5 1005.6 50


Polynuclear Aromatic Hydrocarbons on Pinnacle II™ PAH (Fast LC)

LC_0316

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Environmental


LC_0295

Polynuclear Aromatic Hydrocarbons on Allure™ C18

PPeeaakk LLiisstt:: RReett.. ttiimmee ((mmiinn..)) CCoonncc.. ((µgg//mmLL))1. naphthalene 15.06 5002. acenaphthylene 16.53 10003. acenaphthene 19.12 5004. fluorene 19.12 1005. phenanthrene 20.19 506. anthracene 21.05 507. fluoranthene 22.58 1008. pyrene 23.56 509. benzo(a)anthracene 25.81 50

10. chrysene 25.81 5011. benzo(b)fluoranthene 28.31 10012. benzo(k)fluoranthene 28.72 5013. benzo(a)pyrene 29.62 5014. dibenzo(a,h)anthracene 30.45 10015. indeno(1,2,3-c,d)pyrene 32.07 5016. benzo(ghi)perylene 32.33 100

CCoolluummnn:: AAlllluurree™ CC1188Cat. #: 9164565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 60Å



0.0 4030 10040 10041 4051 40

Flow: 1.0mL/min.Temp.: 30°C (or ambient)Det.: UV @ 254nm

LC_0270

Polynuclear Aromatic Hydrocarbons on Pinnacle II™ C18

PPeeaakk LLiisstt::1. naphthalene2. fluorene3. phenanthrene4. anthracene5. pyrene6. chrysene




(25:75 v/v)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

LC_0269

Polynuclear Aromatic Hydrocarbons on Hypersil® ODS

SSaammppllee::Inj.: 5µLConc.: 100µg/mL eachSample diluent: acetonitrile

CCoolluummnn:: Hypersil® ODSDimensions: 150 x 4.6mmParticle size: 5µmPore size: 120Å




SSaammppllee::Inj.: 5µL 610 PAH Calibration Mix B

(cat.#31455), diluted 1:1 with acetonitrile

Sample diluent: methylene chloride:methanol:acetonitrile(25:25:50 v/v/v)

Compare to the performance on a Hypersil® ODS column.

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Phone: 800-356-1688 or 814-353-1300www.restek.comPhone: 800-356-1688 or 814-353-1300www.restek.comPhone: 800-356-1688 or 814-353-1300www.restek.com

Environmental / General (Test Mixes)


PPeeaakk LLiisstt:: CCoonncc..1. uracil 0.05mg/mL2. aniline 0.75mg/mL3. 2-nitroaniline 0.35mg/mL4. 2,4-nitroaniline 0.35mg/mL5. 3,5-xylenol 2.5mg/mL6. anisole 2.5mg/mL7. toluene 3.5mg/mL

Neutral/Base Mix on Pinnacle™ DB C18

LC_0264

SSaammppllee::Inj.: 2µLSolvent: methanol:water (50:50 v/v)

CCoolluummnn:: PPiinnnnaaccllee™ DDBB CC1188Cat. #: 9414565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 140Å

CCoonnddiittiioonnss::Mobile phase: 50mM KH2PO4, pH 3.5:methanol


Acid/Base Mix on Ultra IBD (pH 7)

0 5 10 15 20 25 min.

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. uracil 52. benzoic acid 503. maleate 54. trimipramine 505. nortriptyline 506. amitriptyline 50

LC_0056

1

2

3

4

5

6


CCoonnddiittiioonnss::Mobile phase: 20mM KxHyPO4, pH 7:

acetonitrile (65:35, v/v)Flow: 1.0mL/min.Temp.: 30°CDet.: UV @ 254nm

Acids and Bases on Ultra IBD (pH 3)

0 2 4 6 8 min.

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. uracil 52. unknown3. maleate 54. benzoic acid 505. nortriptyline 506. amitriptyline 507. trimipramine 50

LC_0057

1

2 3

4

56

7

CCoolluummnn:: UUllttrraa IIBBDDCat. #: 9175565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100ÅCCoonnddiittiioonnss::Mobile phase: 20mM KH2PO4 pH 3:

acetonitrile (70:30, v/v)Flow rate: 1.0mL/min.Temp.: 30°CDet.: UV @ 254nm

0 2 4 6 8 10

1

2

34

5

6

min.

Polynuclear Aromatic Hydrocarbons on Pinnacle™ DB C18





(25:75 v/v)Flow: 1.0mL/minTemp.: ambient Det.: UV @ 254nm

LC_0268


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LC_0266

Neutral/Base Mix on Pinnacle II™ C18

PPeeaakk LLiisstt:: RReett.. ttiimmee ((mmiinn..)) ccoonncc..1. uracil 1.642 0.05mg/mL2. aniline 2.100 0.75mg/mL3. 2-nitroaniline 2.758 0.35mg/mL4. 2,4-dinitroaniline 3.109 0.35mg/mL5. 3,5-xylenol 3.972 2.5mg/mL6. anisole 4.577 2.5mg/mL7. toluene 7.853 3.5mg/mL

SSaammppllee::Inj.: 2µLSolvent: methanol:water

(50:50 v/v)


CCoonnddiittiioonnss::Mobile phase: 50mM KH2PO4 pH 3.5:

methanol (35:65 v/v)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

LC_0262

Acid/Base Mix on Pinnacle II™ C18

PPeeaakk LLiisstt:: RReett.. ttiimmee ((mmiinn..)) CCoonncc..1. caffeine 2.733 0.5mg/mL2. acetylsalicylic acid 4.588 3.5mg/mL3. benzoic acid 7.738 2.8mg/mL

SSaammppllee::Inj.: 2µLSolvent: methanol:water

(50:50 v/v)


CCoonnddiittiioonnss::Mobile phase: 50 mM KH2PO4

pH 3.5:methanol (40:60 v/v)


0 1 2 3

1 2

3

4 5 6 7 min.�

PPeeaakk LLiisstt:: RReett.. TTiimmee ((mmiinn..)) CCoonncc..1. caffeine 2.525 0.5mg/mL2. acetylsalicylic acid 4.088 3.5mg/mL3. benzoic acid 6.733 2.8mg/mL


CCoolluummnn:: PPiinnnnaaccllee™ DDBB CC1188Cat #: 9414565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 140Å

CCoonnddiittiioonnss::Mobile phase: 5 mM KH2PO4, pH 3.5:methanol (40:60 v/v)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

LC_0260

Acid/Base Mix on Pinnacle™ DB C18

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0 1 2 3 4 5 6 7 min.

12

3 4

5

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. uracil 282. toluene 14003. ethylbenzene 17004. quinizarin 945. amitriptyline 2800

SSaammppllee:: HPLC Performance Test Mix (cat.# 31699)Inj.: 5.0µLSolvent: methanol



phosphate, pH 7.0:methanol (20:80 v/v)


Column Performance Test Mix (NIST SRM870) onPinnacle II™ C18

LC_0242



CCoonnddiittiioonnss::Mobile phase: 20mM potassium phosphate, pH 7.0:

acetonitrile (80:20 v/v)Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 254nm

PPeeaakk LLiisstt:: RReett.. TTiimmee ((mmiinn..)) CCoonncc.. ((µLL//mmLL))1. pyridine 9.717 0.1

LC_0230

Pyridine on Ultra Aqueous C18

Pyridine/Phenol Test Mix on Ultra C18

LC_0245

PPeeaakk LLiisstt:: CCoonncc..1. uracil 5.0µg/mL2. pyridine 0.1µL/mL3. phenol 1.86mg/mL

SSaammppllee::Inj.: 5.0µLSolvent: mobile phase

CCoolluummnn:: UUllttrraa CC1188Cat. #: 9174565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100Å



Pyridine/Phenol Test Mix on Pinnacle II™ C18

LC_0247

PPeeaakk LLiisstt:: CCoonncc..1. uracil 5.0µg/mL2. pyridine 0.1µL/mL3. phenol 1.86mg/mL





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LC_0248

Pyridine/Phenol Test Mix on Pinnacle™ DB C18

PPeeaakk LLiisstt:: RReett.. TTiimmee ((mmiinn..)) CCoonncc..1. uracil 1.643 5.0µg/mL2. pyridine 3.119 0.1µL/mL3. phenol 6.969 1.86mg/mL








phosphate, pH 7.0:acetonitrile (80:20 v/v)


LC_0232

Pyridine/Phenol Test Mix on Ultra IBD

PPeeaakk LLiisstt:: RReett.. TTiimmee ((mmiinn..)) CCoonncc..1. uracil 1.812 50µg/mL2 pyridine 4.528 0.1µL/mL3. phenol 8.576 1.86mg/mL

Reversed Phase Test Mix on Pinnacle™ DB C18

LC_0249

PPeeaakk LLiisstt:: CCoonncc..1. uracil 0.02mg/mL2. benzene 3.0mg/mL3. naphthalene 0.5mg/mL4. biphenyl 0.06mg/mL

SSaammppllee::Inj.: 5µL reversed phase test mixSolvent: water:methanol (25:75 v/v)


CCoonnddiittiioonnss::Mobile phase: water:methanol (20:80 v/v)Run Time: 8 min.Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 254nm

free literatureFor more information aboutPinnacle DB columns, requestapplications note ##5599774422.

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CCoonnddiittiioonnss::Mobile phase: water:methanol (20:80 v/v)Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 254nm

Reversed Phase Test Mix on Ultra Aqueous C18

LC_0233

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. uracil 0.022 benzene 3.003. naphthalene 0.504. biphenyl 0.06

SSaammppllee::Inj.: 1.3µLSolvent: methanol:water (75:25, v/v)

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. uracil 0.022 benzene 3.003. naphthalene 0.504. biphenyl 0.06


LC_0234

Reversed Phase Test Mix on Ultra IBD

CCoolluummnn:: UUllttrraa IIBBDDCat #: 9175565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100Å


1

2

3

4

0 1 2 3 4 5 6 7 min.

LC_0201

Reversed Phase Test Mix on Pinnacle II™ C18 (3µm)

PPeeaakk LLiisstt:: ccoonncc..:: ((mmgg//mmLL))1. uracil 0.022. benzene 3.03. napthalene 0.54. biphenyl 0.06

SSaammppllee::Inj.: 5µLSolvent: methanol:water (75:25, v/v)



(20:80)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

0 1 2 3 4

1

2

3

4

5 min

PPeeaakk LLiisstt:: CCoonncc..1. uracil 0.02mg/mL2. benzene 3mg/mL3. naphthalene 0.5mg/mL4. biphenyl 0.06mg/mL




LC_0280

Reversed Phase Test Mix on Pinnacle™ DB C18

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Reversed Phase Test Mix on Pinnacle II™ C18



PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. uracil 0.022. benzene 3.003. naphthalene 0.504. biphenyl 0.06

SSaammppllee::Inj.: 5µLSolvent: water:methanl (25:75, v/v)

LC_0162

LC_0278

Pyridine, N,N-Dimethylaniline, Benzophenone on Pinnacle II™ C18

SSaammppllee::Inj.: 5µLSolvent: water:methanol (50:50 v/v)

CCoolluummnn:: PPiinnnnaaccllee IIII™™ CC1188Cat. #: 9214565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: water:

methanol(40:60 v/v)


PPeeaakk LLiisstt:: CCoonncc..1. uracil 1µg/mL2. pyridine 0.64mg/mL3. n,n-dimethylaniline 0.45mg/mL4. benzophenone 0.59mg.mL

LC_0286

SRM 870 Mix on Pinnacle II™ C18

PPeeaakk LLiisstt:: CCoonncc..1. uracil 28µg/mL2. toluene 1.4mg/mL3. ethylbenzene 1.7mg/mL4. quinizarin 94µg/mL5. amitriptyline 2.8mg/mL


CCoolluummnn:: PPiinnnnaaccllee IIII™™ CC1188Cat. #: 9214565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: 5mM potassium phosphate

pH 7:methanol (20:80 v/v)Flow: 2.0mL/min.Temp.: ambientDet.: UV @ 254nm


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LC_0284

SRM 870 Mix on Pinnacle™ DB C18

PPeeaakk LLiisstt:: CCoonncc..1. uracil 28µg/mL2. toluene 1.4mg/mL3. ethylbenzene 1.7mg/mL4. amitriptyline 2.8mg/mL5. quinizarin 94µg/mL


CCoolluummnn::PPiinnnnaaccllee DDBB CC1188Cat. #: 9414565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 140Å

CCoonnddiittiioonnss::Mobile phase: 5 mM

potassium phosphate pH 7:methanol (20:80 v/v)

Flow: 2.0 mL/min.Temp.: ambientDet.: UV @

254nm

480nm

254nm

210nm

Pyridine/Phenol on Ultra IBD (pH7)

0 2 4 6 8 min.

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. uracil 52. pyridine 503. phenol 300

LC_0058

1

2

3



CCoonnddiittiioonnss::Mobile phase: 20mM KxHy PO4

pH 7:acetonitrile (80:20, v/v)

Flow rate: 1.2mL/min.Temp.: 30°CDet.: UV @ 254nm

Same performanceas Hypersil® BDS

LC_0283

480nm

254nm

210nm

SRM 870 mix on BDS Hypersil® BDS C18

PPeeaakk LLiisstt:: CCoonncc..1. uracil 28 µg/mL2. toluene 1.4 mg/mL3. ethylbenzene 1.7 mg/mL4. quinizarin 94 µg/mL5. amitriptyline 2.8mg/mL

SSaammppllee::Inj.: 5µLSample diluent: methanol

CCoolluummnn::HHyyppeerrssiill® BBDDSS CC1188Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 130Å

CCoonnddiittiioonnss::Mobile phase: 5 mM

potassium phosphate pH 7:methanol (20:80 v/v)



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General (Test Mixes)

PPeeaakk LLiisstt:: CCoonncc..1. uracil 1µg/mL2. pyridine 0.64mg/mL3. N,N-dimethylaniline 0.45mg/mL4. benzophenone 0.59mg/mL




0 2 4 6

1

23

4

8 10 min.

LC_0276

Uracil, Pyridine, N,N-Dimethylaniline, Benzophenone Test Mix on Pinnacle™ DB C18

Normal Phase Test Mix #1 on Pinnacle II™ 5µm Silica

CCoolluummnn:: PPiinnnnaaccllee II™ SSiilliiccaaCat. #: 9210565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: hexane:isopropanol


PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. benzene 1.0 2 benzaldehyde 0.043. benzyl alcohol 3.04. 4-methoxybenzylalcohol 2.0

SSaammppllee::Inj.: 5µLSolvent: hexane

LC_0163 LC_0200

Normal Phase Test Mix on Pinnacle II™ 3µm Silica

1

2

3

4

0 1 2 3 4 5 6 min.

PPeeaakk LLiisstt:: ccoonncc..:: ((mmgg//mmLL))1. benzene 1.02. benzaldehyde 0.043. benzyl alcohol 3.04. 4-methoxybenzyl alcohol 2.0

SSaammppllee::Inj.: 5µLConc.: see peak listSolvent: hexane

CCoolluummnn:: PPiinnnnaaccllee II™ SSiilliiccaaCatalog #: 9210365Dimensions: 150 x 4.6mmParticle size: 3µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: hexane:isopropyl alcohol (96:4)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

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0 1 2 3 4 5 min.

1

2

3

4

LC_0184

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. benzene 1.02 benzaldehyde 0.043. benzyl alcohol 3.04. 4-methoxybenzyl alcohol 2.0

SSaammppllee::Inj.: 5µLSolvent: hexane

CCoolluummnn:: PPiinnnnaaccllee II™ CCyyaannooCat. #: 9216565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: hexane:isopropyl alcohol

(96:4, v/v)Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 254nmPressure: 28 bar

Normal Phase Test Mix on Pinnacle II™ Cyano

LC_0187

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. uracil 0.022 benzene 3.03. naphthalene 0.5 4. biphenyl 0.06



CCoonnddiittiioonnss::Mobile phase: methanol::water (65:35, v/v)Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 254nmPressure: 130 bar

Test Mix on Pinnacle II™ Phenyl

0 1 2 3 4 min.

1

2

3

4

LC_0183

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. uracil 0.022 benzene 3.03. naphthalene 0.5 4. biphenyl 0.06

SSaammppllee::Inj.: 1.3µLSolvent: methanol:water (75:25 v/v)


CCoonnddiittiioonnss::Mobile phase: methanol:water (80:20 v/v)Flow: 1.0 mL/min.Temp.: ambientDet.: UV @ 254nmPressure: 116 bar

Reversed Phase Test Mix on Pinnacle II™ C8

0 2

1

4 6 8 min.

PPeeaakk LLiisstt::1. PCMX (4-chloro-3,5-dimethylphenol)

SSaammppllee::Inj.: 10µL of 5% solution of hand soapSolvent: methanol



LC_0293

PCMX in Hand Soap on Pinnacle™ DB C18

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Dietary Supplements / Plant Extracts


LC_0174

0 2 4 6 8 min.

1

Allicin in Garlic Tablets on Pinnacle II™ C18

PPeeaakk LLiisstt::1. allicin



(50:50 v/v)Flow: 0.8 mL/min.Temp.: 30°C Det.: UV @ 240nm

Cabbage Extract on Ultra Aqueous C18



phosphate, pH 2.5B: acetonitrileTTiimmee ((mmiinn..)) %% BB

0 010 7511 016 0


PPeeaakk LLiisstt::1. phenethyl

glucosinolate

LC_0165

Phenethyl Glucosinolate on Ultra Aqueous C18




TTiimmee ((mmiinn..)):: %% BB0.0 0

10 7511 016 0


PPeeaakk LLiisstt::1. phenethyl glucosinolate

SSaammppllee::Inj.: 10µLConc.: 1000µg/mLSolvent: water

LC_0166

PPeeaakk LLiisstt::1. hyperforin2. adhyperforin

SSaammppllee:: St. John’s wort capsuleInj.: 20µLConc.: 4.17mg/mLSolvent: methanolAutosampler: 15°C

Hyperforin in St. John’s Wort on Pinnacle II™ C18


CCoonnddiittiioonnss::Mobile phase: 0.01N phosphate buffer, pH 2.5:

acetonitrile (15:85, v/v)Flow: 1.2mL/min.Temp.: 30°CDet.: UV @ 270nm

LC_0175

SSaammppllee::Inj.: 20µLConc.: 1.2mg/mL

garlic tablet in waterSolvent: deionized water


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498 Dietary Supplements / Plant Extracts


LC_0198

1

2

3

4

0 2 4 6 8 10 12 14 16 18 min.

Phenolic Antioxidants on Pinnacle II™ C18

PPeeaakk LLiisstt:: ccoonncc.. ((ppppmm))1. propyl gallate 1682. TBHQ 1823. 2-BHA + 3-BHA 1974. BHT 193


CCoolluummnn:: PPiinnnnaaccllee II™ CC1188Cat. #: 9214565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: A = 1% acetic acid

B = methanolTTiimmee BB((mmiinn..)) ((%%))

0 504 5010 9025 9026 50


Phenolics in Echinacea on Pinnacle II™ C18

LC_0195

CCoolluummnn:: PPiinnnnaaccllee II™ CC1188Cat. #: 9214565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: A = 0.1% phosphoric acid

B = acetonitrileTTiimmee ((mmiinn..)) %%BB

0 1013 2214 4014.5 4015 1020 10


PPeeaakk LLiisstt::1. caftaric acid2. chlorogenic acid3. echinacoside4. cichoric acid

SSaammppllee:: Echinacea capsule Inj.: 10µLConc.: 6.7 mg/mL capsule

contents in sample diluentSolvent: ethanol:water (70:30, v/v)Sample temp.: 25°C

1

23

4

0 2 4 6 8 10 12 14 min.

PPeeaakk LLiisstt:: RReett.. TTiimmee ((mmiinn..))1. oxalic acid 2.8982. pyruvic acid 3.9683. malic acid 4.0704. citric acid 7.644

SSaammppllee::Inj.: 20µLSolvent: 0.4N sulfuric acid

Tobacco Extract on Ultra Aqueous C18




LC_0172

497-527_LCAPP.qxd 11/3/2004 2:52 PM Page 498

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499Dietary Supplements / Plant Extracts


1

2

3

4

0 2 4 6 8 10 12 14 min.

LC_0197

Tocopherols in Soy Oil on Pinnacle II™ Silica

PPeeaakk LLiisstt::1. α-tocopherol2. β-tocopherol3. γ-tocopherol4. δ-tocopherol

SSaammppllee::Inj.: 10µLConc.: approx. 1.25% soy oilSolvent: hexane

CCoolluummnn:: PPiinnnnaaccllee II™ SSiilliiccaaCat. #: 9210565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: isopropyl alcohol:hexane

(0.5:99.5)Flow: 0.6 mL/min.Temp.: 30°C Det.: UV @ 295nm

0 2 4 6 8 10 12 14 min.

1

2

3

4

5 6 9

78

PPeeaakk LLiisstt:: CCoonncc..:: ((mmgg//mmLL))1. thiamin (B1) 2502. ascorbic acid (C) 10003. unknown n/a4. nicotinic acid (B3) 10005. unknown n/a6. pantothenic acid (B5) 10007. folic acid (B9) 5008. riboflavin (B2) 2509. methyl paraben 0.2

Vitamins (Water Soluble) on Ultra Aqueous C18


CCoonnddiittiioonnss::Mobile Phase: A: 25mM potassium phosphate, pH

2.00:methanol (95:5, v/v)B: methanol:25mM potassium phosphate, pH 3.5

(60:40, v/v)

TTiimmee ((mmiinn..)) %% BB0-6 06.01 2511 10016 100


LC_0141

SSaammppllee::Initial dilutions of B1 and B2

basified with ammonium hydroxide

free literatureFor more information about dietary supplementanalysis, request applications note lliitt.. ccaatt..## 5599336644,Analyzing Nutraceutical Products by Liquid & GasChromatography.

497-527_LCAPP.qxd 11/3/2004 2:52 PM Page 499

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500 Dietary Supplements / Plant Extracts


PPeeaakk LLiisstt:: CCoonncc.... ((mmgg//mmLL))1. solvent front n/a2. menadione (vitamin K3) 0.453. all-trans-retinol (vitamin A) 0.344. vitamin D3 0.45. unknown n/a6. α tocopherol (vitamin E) 2.47. α tocopherol acetate

(vitamin E acetate) 2.48. unknown n/a9. phylloquinone (vitamin K1) 0.84

Vitamins (Fat Soluble) on Ultra C18

CCoolluummnn:: UUllttrraa CC1188Cat. #: 9174575 Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: acetonitrile:methanol

(90:10, v/v)TTiimmee ((mmiinn..)) FFllooww ((mmLL//mmiinn..))

1.00 1.005.00 1.005.01 2.00

50.0 2.00Temp.: 30°CDet.: UV @ 280nm

1

2

3

4

5

67

8

9

0 10 20 30 40 min.LC_0144


CCoolluummnn:: UUllttrraa AAqquueeoouuss CC1188Cat. #: 9178565 Dimensions: 150 x 4.6mmParticle Size: 5µmPore Size: 100Å


phosphate, pH 2.5Flow: 1.0 mL/min.Temp.: ambientDet.: UV @ 254nm

LC_0226

Vitamins Thiamin (Vitamin B1) and Ascorbic Acid(Vitamin C) on Ultra Aqueous C18

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. ascorbic acid 2.52. thiamin 2.5





LC_0227

Vitamins Thiamin (Vitamin B1) and Ascorbic Acid(Vitamin C) on Ultra IBD

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. thiamin 2.52. ascorbic acid 2.5

Watercress Extract on Ultra Aqueous C18




TTiimmee ((mmiinn..)) %% BB0.0 010 7511 016 0


PPeeaakk LLiisstt::1. phenethyl glucosinolate

LC_0167

SSaammppllee:: Solvent: diethyl ether

SSaammppllee:: watercress extractInj.: 100µLSolvent: water

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501Foods & Flavors


LC_0155


CCoonnddiittiioonnss::Mobile phase: A: 1% acetic acid in water


0 502 509 8012 8013 50


PPeeaakk LLiisstt::1. capsaicin

SSaammppllee:: Inj.: 10µLConc.: 110ppm capsaicinSolvent: ethanol

Capsaicinoids: Heat Level Standard on Ultra C18

0 2 4 6 8 10 min.

1

0 1 2 3 4 5 6 7 min.

1

LC_0181

Black Pepper: Piperine on Pinnacle II™ Cyano

PPeeaakk LLiisstt::1. piperine

SSaammppllee::Inj.: 5µLConc.: 100µg/mLSolvent: methanol


CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (50:50 v/v)Flow: 1.0mL/min.Temp.: ambient Det.: UV/Vis @ 345nm

Capsaicinoids: Heat Level Assay for Habeñero Nuggetson Ultra C18


CCoonnddiittiioonnss::Mobile phase: A: 1% acetic acid in water


0 502 509 80

12 8013 50


PPeeaakk LLiisstt::1. unknown2. capsaicin3. dihydrocapsaicin

SSaammppllee::Inj.: 10µL habañero nugget extractConc.: 0.8g sample in 200mLSolvent: ethanol

LC_0156

0 2 4 6 8 10 min.

1

2

3

Capsaicinoids: Heat Level Assay for Habeñero Nuggets on Pinnacle II™ C18


CCoonnddiittiioonnss::Mobile phase: A: 1% acetic acid in water (v/v)


0 502 509 8012 8013 50


PPeeaakk LLiisstt::1. unknown2. capsaicin3. dihydrocapsaicin

SSaammppllee::Inj.: 10µL habañero nugget extractConc.: 4000µg/mLSolvent: ethanol

LC_0160

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0 1 2 3 4 5 min.

1

2

3

4

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502 Foods & Flavors/Antimicrobial Agents


LC_0150

SSaammppllee::Inj.: 10µLConc.: 100 ppm sorbic acid,

200 ppm benzoic acidSolvent: mobile phase

CCoolluummnn:: UUllttrraa PPhheennyyllCat. #: 9105565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: 1% acetic acid:methanol

(80:20, v/v)Flow: 1.2 mL/min.Temp.: ambientDet.: UV @ 245nm

PPeeaakk LLiisstt::1. sorbic acid2. benzoic acid

Sorbic and Benzoic Acids on Ultra Phenyl

0 2 4 6 8 10 12 14 16 18 min.

2

1

LC_01990 2 4 6 8 10 12 14 16 18 min.

1

2

Sorbic and Benzoic Acids on Pinnacle II™ Phenyl

CCoolluummnn:: PPiinnnnaaccllee II™ PPhheennyyllCat. #: 9215565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: 1% acetic acid:methanol (80:20)Flow: 1.0 mL/min.Temp.: 25°C Det.: UV @ 230nm

PPeeaakk LLiisstt::1. sorbic acid2. benzoic acid

SSaammppllee::Inj.: 10µLConc.: 100ppm eachSolvent: methanol

LC_0176

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. methyl paraben 102. ethyl paraben 203. propyl paraben 304. butyl paraben 40



CCoonnddiittiioonnss::Mobile phase: 0.1% acetic acid in water:

acetonitrile (50:50, v/v)Flow: 1.0mL/min.Temp.: ambient Det.: UV @ 254nm

Parabens on Pinnacle II™ C8

LC_0177

Parabens on Pinnacle II™ Phenyl


CCoonnddiittiioonnss::Mobile phase: 0.1% acetic acid in water:

acetonitrile (50:50, v/v)Flow: 1.0 mL/min.Temp.: ambient Det.: UV @ 254nm

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503Foods & Flavors


LC_0311

Organic Acids (Isocitric and Malic) on Allure™ Organic Acids

PPeeaakk LLiisstt::1. malic acid 2. isocitric acid

SSaammppllee::Inj.: 5µLConc.: approx. 0.2 mg/mL malic acid;

0.02 mg/mL isocitric acidSolvent: water

CCoolluummnn:: AAlllluurree™ OOrrggaanniicc AAcciiddssCat. #: 9165575Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 60Å

CCoonnddiittiioonnss::Mobile phase: 100mM KH2PO4, pH 2.5Flow: 0.5mL/min.Temp.: ambientDet.: UV @ 226nm



Carboxylic Acids in 100% Aqueous Mobile Phase on Ultra Aqueous C18



phosphate, pH 2.5Flow rate: 1.0mL/min.Temp.: 25°CDet.: UV @ 210nm

0 1 2 3 4 5 6 min.

1

2

3

4

AA.. iinniittiiaallBB.. aafftteerr 33 DDaayyss

LC_0142

PPeeaakk LLiisstt::1. quinic acid2. malic acid3. citric acid

SSaammppllee::Inj.: 10µL Conc.: cranberry juice:water (50:50, v/v)Solvent: water

CCoolluummnn:: AAlllluurree™ OOrrggaanniicc AAcciiddssCat. #: 9165585 Dimensions: 300 x 4.6mmParticle size: 5µmPore size: 60Å

CCoonnddiittiioonnss::Mobile phase: 100mM phosphate buffer, pH 2.5Flow: 0.5mL/min.Temp.: ambientDet.: UV @ 226nm

Organic Acids in Cranberry Juice on Allure™ Organic Acids

LC_0236

restekinnovation!Excellent peak symmetry evenwith a 100% aqueous mobilephase!


PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))::1. malonic acid 5002. lactic acid 5003. acetic acid 10004. citric acid 10005. succinic acid 20006. fumaric acid 10




phosphate, pH 2.5: acetonitrile (99:1)


LC_0140

1

2

3

4

5

6

0 1 2 3 4 min.

restekinnovation!Excellent peak symmetry evenwith a 100% aqueous mobilephase!

497-527_LCAPP.qxd 11/3/2004 2:52 PM Page 503

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504 Foods & Flavors


PPeeaakk LLiisstt::1. tartaric acid2. malic acid

SSaammppllee::Inj.: 10µL Conc.: grape juice:water (50:50, v/v)Solvent: purified water

CCoolluummnn:: AAlllluurree™™ OOrrggaanniicc AAcciiddssCat. #: 9165585Dimensions: 300 x 4.6mmParticle size: 5µmPore size: 60Å


Organic Acids in Grape Juice on Allure™ Organic Acids

LC_0237

restekinnovation!Excellent peak symmetry in100% aqueous mobile phase!

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. tartaric acid 12. quinic acid 13. malic acid 14. citric acid 15. fumaric acid 0.005

SSaammppllee::Inj.: 10µL standard solutionSolvent: water

CCoolluummnn:: AAlllluurree™ OOrrggaanniicc AAcciiddssCat. #: 9165585Dimensions: 300 x 4.6mmParticle size: 5µmPore size: 60Å


Organic Acids Standard on Allure™ Organic Acids

LC_0238

restekinnovation!Baseline resolution of tartaricand quinic acids in 100%aqueous mobile phases!

LC_0297

Carboxylic Acids (Pyruvic and Lactic) on Ultra™ Aqueous C18


1. pyruvic acid 52. lactic acid 50

SSaammppllee::Inj.: 5µLSolvent: 50mM potassium

phosphate, pH 2.5

CCoolluummnn:: UUllttrraa™ AAqquueeoouuss CC1188Cat. #: 9178565 Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100Å


phosphate, pH 2.5Flow: 1.0mL/min.Temp.: 26°CDet.: UV @ 210nm

Diet Cola on Pinnacle II™ C18

CCoolluummnn:: PPiinnnnaaccllee II™ CC1188Cat.#: 9214565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å


phosphate monobasic with 0.1% v/v TEA, pH 2.9 B: acetonitrileTTiimmee ((mmiinn..)) %% BB

0.00 204.99 205.00 759.99 75

10.00 20Flow: 1.0mL/min.Temp.: 27°CDet.: UV @ 210nm

0 1 2 3 4 5 6 7 8 9 min.

1

PPeeaakk LLiisstt::1. citric acid2. caffeine3. aspartame4. benzoic acid and other components

SSaammppllee::Inj.: 5.0µLSolvent: raw sample diluted (50:50, v/v) with

mobile phase A, filtered, and injected.2

3

4

LC_0153

restekinnovation!Excellent peak symmetry in100% aqueous mobile phase!

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505Foods & Flavors


LC_0202

0 2 4 6 8 10 min.

1

23

4 5

PPeeaakk LLiisstt::1. fructose2 glucose3. sucrose4. maltose5. lactose

SSaammppllee::Inj.: 5.0µLConc.: 15mg/mL eachSolvent: methanol:water (10:90, v/v)

CCoolluummnn:: PPiinnnnaaccllee II™ AAmmiinnooCat. #: 9217565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (25:75, v/v)Flow: 1.0mL/min.Temp.: 35°CDet.: refractive index @ 35°C

Sugars on Pinnacle II™ Amino

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. fructose 2.02. glucose 2.13. sucrose 4.04. maltose 4.55. lactose 4.4

LC_0223

Sugars on Pinnacle II™ Amino (3µm)

CCoolluummnn:: PPiinnnnaaccllee II™ AAmmiinnooCat. #: 9217365Dimensions: 150 x 4.6mm Particle size: 3µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (25:75, v/v)Flow: 1.5 mL/min.Temp.: 35°C Det.: refractive index @ 35°C

LC_01590 2 4 6 8 10 12 14 min.

Sugars: Maple Flavored Syrup on Ultra Amino

CCoolluummnn:: UUllttrraa AAmmiinnooCat. #: 9107365Dimensions: 150 x 4.6mmParticle size: 3µmPore size: 100Å


(25:75, v/v)Flow: 0.8mL/min.Temp.: 35°CDet.: refractive index

@ 35°C

PPeeaakk LLiisstt::1. fructose2. glucose3. sucrose

SSaammppllee:: Inj.: 20µLConc.: 10% solution of

maple syrupSolvent: acetonitrile:water

(70:30, v/v)

1

23

Sugars on Ultra Amino

PPeeaakk LLiisstt:: CCoonncc..((mmgg//mmLL))1. fructose 2.02. glucose 2.13. sucrose 4.04. maltose 4.55. lactose 4.4

LC_0154

0 2 4 6 8 10 12 14 min.

1

2

3

4

5


CCoolluummnn:: UUllttrraa AAmmiinnooCat. #: 9107365Dimensions: 150 x 4.6mmParticle size: 3µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (25:75, v/v)Flow: 0.8mL/min.Temp.: 35°C Det.: refractive index @ 35°C


497-527_LCAPP.qxd 11/3/2004 2:52 PM Page 505


CCoonnddiittiioonnss::Mobile phase: A: 10mM sodium phosphate

monobasic in water, pH 4.8B: acetonitrile (22A:78B, v/v)

Flow: 1.3mL/min.Temp.: 40°C Det.: refractive index @ 40°C

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Foods & Flavors


LC_0204

Sugars: 100% Maple Syrup on Pinnacle II™ Amino (high sensitivity)

0 2 4 6 8 10 12 14 min.

12

3


SSaammppllee::Inj.: 100.0µLConc.: 1% maple syrup

in solventSolvent: acetonitrile:water

(70:30, v/v)



LC_0205

Sugars: 100% Maple Syrup on Pinnacle II™ Amino (low sensitivity)

1 2

3

0 2 4 6 8 10 12 14 min.


SSaammppllee::Inj.: 50.0µLConc.: 1% maple syrup


(70:30, v/v)


CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (25:75)Flow: 1.0mL/min.Temp.: 35°C Det.: refractive index @ 35°C

1

0 2 4 6 8 10 min.

LC_0206

Sugars: Skim Milk on Pinnacle II™ Amino



PPeeaakk LLiisstt::1. lactose

SSaammppllee::Inj.: 10.0µL 10% skim milk in solventSolvent: acetonitrile:water (70:30, v/v)

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL)) RReett.. TTiimmee ((mmiinn..)) RRRRTT RReess.. IInnjj.. %% RRSSDD ((nn==55))1. fructose 0.4 4.6 0.44 — —2. galactose 6.4 6.1 0.58 — —3. epi-lactose unknown 10.0 0.95 — —4. lactulose 40 10.5 1.00 1.1 0.3%5. lactose 4.8 12.7 1.21 2.9 —

SSaammppllee::Inj.: 20µL resolution standardSolvent: acetonitrile:water (50:50)

LC_0222

Laxative Lactulose Concentrate by USP 25: ResolutionSolution on Pinnacle II™ Amino

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507Foods & Flavors


1

2

0 1 2 3 4 5 6 7 min.

LC_0203

Sugars: Cola on Pinnacle II™ Amino



(25:75, v/v)Flow: 1.0mL/min.Temp.: 35°CDet.: refractive index @ 35°C

PPeeaakk LLiisstt::1. fructose2. glucose

SSaammppllee::Inj.: 10.0µL 10% cola


(70:30, v/v)1

2

0 1 2 3 4 5 6 7 min.

LC_0207

Sugars: Orange Soda on Pinnacle II™ Amino

PPeeaakk LLiisstt::1. fructose 2. glucose

SSaammppllee::Inj.: 20.0µL 10% orange

soda in solventSolvent: acetonitrile/water

(70:30, v/v)


CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (25:75)Flow: 1.0mL/min.Temp.: 35°C (or ambient)Det.: refractive index @ 35°C

Vanillin and Ethyl Vanillin on Ultra C8


CCoonnddiittiioonnss::Mobile phases: A: 1.2% acetic acid

B: methanolTTiimmee ((mmiinn..)) %%BB

0.0 20.05.0 20.0

15.0 40.020.0 40.025.0 20.0


0 2 4 6 8 10 12 14 16 18 min.

2

1

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. vanillin 0.122. ethyl vanillin 0.04

SSaammppllee::Inj.: 10µLSolvent: 40% ethanol

LC_0148 LC_0251

Vanillin and Ethyl Vanillin on Pinnacle™ DB C18 (Fast LC)

PPeeaakk LLiisstt::1. vanillin2. ethyl vanilling/mL each

componentSample Diluent: 4% v/v ethanol

in water

SSaammppllee::Inj.: 5µLConc.: 200µg/mL eachSample Diluent: 4% v/v ethanol in water


CCoonnddiittiioonnss::Mobile phase: 0.2% v/v phosphoric

acid in water:methanol(80:20 v/v)

Flow: 2mL/min.Temp.: 27°CDet.: UV @ 254nm

497-527_LCAPP.qxd 11/3/2004 2:52 PM Page 507

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508 Foods & Flavors


LC_0252

Vanilla Bean Extract on Pinnacle™ DB C18 (Fast LC)

PPeeaakk LLiisstt RReett.. TTiimmee ((mmiinn..))

1. vanillin 2.00


CCoonnddiittiioonnss::Mobile phase: 0.2% v/v phosphoric acid in

water:methanol (80:20 v/v)Flow: 2mL/min.Temp.: 27°C Det.: UV @ 254nm

LC_0253

Vanilla Flavoring (Imitation) on Pinnacle™ DB C18 (Fast LC)

CCoolluummnn:: PPiinnnnaaccllee DDBB CC1188Cat. #: 9414554Dimensions: 50 x 4.0mmParticle size: 5µmPore size: 140Å

CCoonnddiittiioonnss::Mobile phase: 0.2% v/v phosphoric acid in

water:methanol (80:20 v/v)Flow: 1.5mL/min.Temp.: 27°C Det.: UV @ 254nm

PPeeaakk LLiisstt:: RReett.. TTiimmee (min.)1. Vanillin 2.00

SSaammppllee::Inj.: 5µLDiluent: 20% v/v commercial

flavoring in water

SSaammppllee::Inj.: 5µLDiluent: 20% v/v

commercial extract in water

a plus 1™ story“When first seeking help with application issues it was suggested to me from a friend that I call Restek. Hisexperience was that the support group from Restek was very willing to assist and that they might possibly shipme some phase to try out as opposed to spending a large sum of money on a new column phase that was nobetter then what I already had been using. After giving Restek a call, the customer support person sent mesome CarboFrits free of charge to see if it would benefit my chromatography. I have since experienced manyhours of useful consultation that has benefited my organization in numerous ways. On every occasion where Icalled for assistance it was always followed by a call later to check and see if things were going as planned.Thank you Restek for your caring support group of personnel.”

RR.. FFrraannkk HHaarrtt IIIIII,, Lab Tech, GenApps research facility

497-527_LCAPP.qxd 11/3/2004 2:52 PM Page 508

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509


Pharmaceutical


Analgesic Acetaminophen on Pinnacle II™ C18

0 0.5 1 1.5 2 2.5 3 3.5 4 min.

1

PPeeaakk LLiisstt::1. acetaminophen

SSaammppllee::Inj.: 1.0µLConc.: 1.2 mg/mLSolvent: mobile phase


CCoonnddiittiioonnss::Mobile phase: 10mM potassium phosphate

monobasic, pH 3.0:acetonitrile (90:10)


LC_0151

Analgesic Acetaminophen and Narcotic AnalgesicCodeine Phosphate on Pinnacle II™ C18


CCoonnddiittiioonnss::Mobile phase: 10mM potassium phosphate monobasic

with 0.1% v/v TEA, pH 3.0:acetonitrile (90:10, v/v)


PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. acetaminophen 12002. codeine phosphate 246

SSaammppllee:: Inj.: 1.0µLSolvent: mobile phase

LC_0152

Analgesic Acetaminophen and Narcotic Analgesics onAllure™ Basix

1

PPeeaakk LLiisstt::1. acetaminophen2. codeine3. oxycodone4. hydroxycodone

SSaammppllee::Inj.: 5µLConc.: 100µg/mLSolvent: mobile phase

CCoolluummnn:: AAlllluurree™ BBaassiixxCat. #: 9161565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 60Å


acetate, pH 4.5:acetonitrile (85:15, v/v)


2

3

0 1 2 3 4 5 6 7 8 9 min.

LC_0082

4

PPeeaakk LLiisstt:: CCoonncc.. (µg/mL) RReett.. TTiimmee (min.) TTaaiilliinngg RReessoolluuttiioonnU. unknown unknown 3.1 NA NA1. morphine sulfate 204 3.3 1.0 1.82. acetaminophen 92 5.0 1.1 14.13. codeine phosphate 216 5.5 1.4 2.64. oxycodone HCl 206 7.5 1.4 8.55. hydrocodone bitartrate 218 8.9 1.4 5.0

LC_0218

Analgesic Acetaminophen and Narcotic Analgesics onUltra C18

CCoolluummnn:: UUllttrraa CC1188Catalog #: 9174575Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: A: 10mm

potassium phosphate, pH 2.8B: acetonitrile:methanol (90:10 v/v)(85A:15B, v/v)


SSaammppllee::Inj.: 4.0µLSample: raw material mixSolvent: mobile phase

497-527_LCAPP.qxd 11/3/2004 2:52 PM 9

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510 Pharmaceutical



www.restek.com

PPeeaakk LLiisstt:: CCoonncc.. (µg/mL)1. morphine sulfate 2042. acetaminophen 923. codeine phosphate 2164. oxycodone HCl 2065. hydrocodone bitartrate 218

LC_0220



CCoonnddiittiioonnss::Mobile phase: A: 10mm

potassium phosphate, pH 2.8B: acetonitrile(90A:10B, v/v)



PPeeaakk LLiisstt:: CCoonncc.. (µg/mL)U. unknown unknown1. morphine sulfate 2042. acetaminophen 923. codeine phosphate 2164. oxycodone HCl 2065. hydrocodone bitartrate 218

LC_0221




CCoonnddiittiioonnss::Mobile phase: A: pH 2.8 10mm

potassium phosphateB: acetonitrile:methanol,

(90:10, v/v)(85A:15B, v/v)


CCoolluummnn:: PPiinnnnaaccllee II™ CC1188Cat. #: 9214575-700Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: A: 0.73g 1-heptane

sulfonic acid and 10mL glacial acetic acid, diluted to 720mL with water, pH=2.33B: methanol (72A:28B, v/v)

Flow: 1.00mL/min,Temp.: 26.5°C Det.: UV @ 284nm

LC_0209

Narcotic Analgesic Morphine Sulfate Raw Material -USP 25: Resolution Solution on Pinnacle II™ C18

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. unknown unknown2. phenol 0.153. morphine sulfate 0.24


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0 1 2 3 4 min.

PPeeaakk LLiisstt:: RReett.. TTiimmee ((mmiinn..)) IInnjj.. %% RRSSDD ((nn==55)) 1. hydrocodone bitartrate 3.1 0.37%

SSaammppllee::Inj.: 20µLConc.: 1µg/mLSample: assay standardSolvent: mobile phase

LC_0211

Narcotic Analgesic Hydrocodone Bitartrate by USP 25 on Pinnacle II™ Silica

CCoolluummnn:: PPiinnnnaaccllee II™ SSiilliiccaaCat. #: 9210575Dimensions: 250 x 4.6mmParticle size: 5µmPore size: 110Å

CCoonnddiittiioonnss::Mobile phase: A: 800mL acetonitrile; 4mL water;

1mL diethylamineB: methanol (55A:45B, v/v)


Impurities resolvedfrom primary peak.

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL)) RReett.. TTiimmee ((mmiinn..)) RRRRTT TTaaiilliinngg RReess..1. codeine phosphate 13 5.7 0.8 1.42. oxycodone HCl 9 7.6 1.0 1.2 5.9

SSaammppllee::Inj.: 10µL resolution solutionSolvent: mobile phase

LC_0215

Narcotic Analgesic Oxycodone HCl Raw Material by USP 25 (modified):Resolution Solution on Ultra C8



phosphate monobasic pH=2.5B: methanol (90A:10B, v/v)

Flow: 1.5mL/min.Temp.: 27°C Det.: UV @ 206nm and 284nm

206nm

284nm

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CCoolluummnn::Cat. #: 9103564Dimensions: 150 x 4.0mmParticle size: 5µmPore size: 100Å

CCoonnddiittiioonnss::Mobile phase: A: 5mM hexane sulfonic acid

with 2mL TEA and 3mL ofphosphoric acid per liter. pH adjusted to 2.5B: methanol (90A:10B, v/v)


Narcotic Analgesic Oxycodone HCl Raw Material byUSP 25: Resolution Solution on Ultra C8

LC_0213

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL)) RReett.. TTiimmee ((mmiinn..)) RRRRTT TTaaiilliinngg RReess..1. codeine phosphate 13 19.3 0.7 1.1 —2. oxycodone HCl 9 26.8 1.0 0.99 3.3

SSaammppllee::Inj.: 10µLSample: resolution solutionSolvent: mobile phase



phosphate monobasic pH=2.5B: methanol (90A:10B, v/v)


LC_0210

Narcotic Analgesic Oxycodone HCI Raw Material by USP25 (modified): Resolution Solution on Pinnacle II™ C8

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL)) RReett.. TTiimmee ((mmiinn..)) RRRRTT TTaaiilliinngg RReess..1. codeine phosphate 13 4.3 0.8 1.5 — 2. oxycodone HCl 9 6.2 1.0 1.1 4.5

SSaammppllee::Inj.: 10µL resolution solutionSolvent: mobile phase

Antiarrhythmic Atenolol on Allure™ Basix

1

PPeeaakk LLiisstt::1. uracil (marker)2. atenolol

SSaammppllee::Inj.: 1µLConc.: 1mg/mLSolvent: water:methanol

(7:3, v/v)


CCoonnddiittiioonnss::Mobile phase: acetonitrile:20mM ammonium

acetate, pH 4.5 (80:20, v/v)Flow rate: 1.2mL/min.Temp.: 25°CDet.: UV @ 225nm

2

0 2 4 6 8 10 12 min.

LC_0072

Antiarrhythmic Digoxin on Ultra IBD

PPeeaakk LLiisstt::1. unknown2. unknown3. digoxin

LC_0068

SSaammppllee::Inj.: 10µLConc.: 1000µg/mLSolvent: water:methanol (1:1, v/v)


CCoonnddiittiioonnss::Mobile phase: water with

0.1% (v/v) acetic acid:acetonitrile (65:35, v/v)


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Antiarrhythmic Calcium Channel Blockers on Ultra Cyano

1

PPeeaakk LLiisstt::1. diltiazem2. nifedipine impurity3. verapamil4. nifedipine5. nicardipine

LC_0062

0 1 2 3 4 5 6 7 8 9 min.

2

3

4

5

SSaammppllee::Inj.: 5µLConc.: 100mg/mLSolvent: acetonitrile:water (1:1)



phosphate monobasic, pH 3.0:acetonitrile (70:30, v/v)

Flow rate: 1.2mL/min.Temp.: 30°C (±1°C)Det.: 235nm

AntiarrhythmicEnalapril Maleate on Allure™ Basix

PPeeaakk LLiisstt::1. maleate2. enalapril

SSaammppllee::Inj.: 2.5µLConc.: 1.1mg/mLSolvent: methanol:water

(30:70)



formate, pH 2.5:acetonitrile(20:80, v/v)


LC_0091

Antiarrhythmic Furosemide on Ultra IBD

PPeeaakk LLiisstt::1. uracil (marker)2. furosemide

SSaammppllee::Inj.: 5µLConc.: 1000µg/mLSolvent: acetonitrile:water

(60:40, v/v)



acetate, pH 4.5:acetonitrile (70:30, v/v)


LC_0071

Impurities in Furosemide(enlargement of baseline)

LC_0250

Digitalis Extracts/Derivatives onUltra PFP PropylCartridge Column (Fast LC)

CCoolluummnn:: CCuussttoomm FFaasstt LLCC CCaarrttrriiddggeeDimensions: 30 x 4.0mmParticle size: 3µmPore size: 100Å


B: acetonitrile

TTiimmee ((mmiinn..)) %%BB0.0 201.5 201.51 353.0 353.1 20


PPeeaakk LLiisstt:: CCoonncc.. RReett.. TTiimmee ((mmiinn..))1. digoxigenin 100µg/mL 0.402. gitoxigenin 100µg/mL 0.803. digoxin 100µg/mL 1.104. gitoxin ~10µg/mL 2.205. digitoxin 100µg/mL 2.60

SSaammppllee::Inj.: 10µLSolvent: water:acetonitrile (80:20 v/v)

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Antiarrhythmic Metoprolol on Allure™ Basix

PPeeaakk LLiisstt::1. unknown2. metoprolol


(30:70)



formate, pH 2.5:acetonitrile (10:90, v/v)


LC_0090

AntiarrhythmicTimolol Maleate on Allure™ Basix

PPeeaakk LLiisstt::1. maleate2. timolol

SSaammppllee::Inj.: 2.5µLConc.: 1.2mg/mLSolvent: methanol:water (30:70)





LC_0092

Antiarrhythmic Triamterene and Hydrochlorothiazide on Allure™ Basix

PPeeaakk LLiisstt::1. hydrochlorothiazide2. triamterene

SSaammppllee::Inj.: 1µLConc.: 500µg/mLSolvent: methanol:water:

tetrahydrofuran(30:35:35)



acetate, pH 4.5:acetonitrile(65:35,v/v)


LC_0086

Antiarrhythmic Triamterene on Allure™ Basix

PPeeaakk LLiisstt::1. uracil (marker)2. triamterene

SSaammppllee::Inj.: 1µLConc.: 1mg/mLSolvent: methanol:

tetrahydrofuran(7:3,v/v)



acetate, pH 4.5:acetonitrile(65:35,v/v)


LC_0074

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Antiarrhythmic Verapamil HCl on Allure™ Basix

1

PPeeaakk LLiisstt::1. toluene (marker)2. verapamil HCl


(70:30, v/v)


CCoonnddiittiioonnss::Mobile phase: acetonitrile:

20mM ammoniumacetate, pH 4.5(65:35,v/v)


2

0 1 2 3 4 5 6 7 8 9 min.

LC_0077

1

0 5 10 15 20 25 30 35 min.

LC_0208

Antiarrhythmic Verapamil HCl Raw Material on Ultra IBD (EP 2002 modified)

PPeeaakk LLiisstt:: RReett.. ttiimmee ((mmiinn..))1. verapamil 15.0

SSaammppllee::Inj.: 20µLConc.: 25µg/mLSolvent: initial mobile phase


CCoonnddiittiioonnss::Mobile phase: A: 40 mM potassium

phosphate monobasic pH 6.25B: acetonitrile

TTiimmee ((mmiinn..)) %%BB0.0 3722.0 3727.0 6535.0 6536.0 3746.0 37

Flow: 1.5 mL/min.Temp.: 25°C Det.: UV @ 278nm

Antiasthmatic Beclomethasone on Ultra Cyano

1

PPeeaakk LLiisstt::1. deschlorobeclomethasone2. beclomethasone

SSaammppllee::Inj.: 10µLConc.: 100µg/mL beclomethasoneSolvent: water:acetonitrile

(9:1, v/v)



(70:30, v/v)Flow rate: 1.0mL/min.Temp.: ambientDet.: UV @ 238nm

2

4 5 6 7 8 9 min.

LC_0070

PPeeaakk LLiisstt::1. beclomethasone2. deschlorobeclomethasone

SSaammppllee::Inj.: 10µLConc.: 79.5µg/mL (beclomethasone)Solvent: water:acetonitrile (10:90, v/v)


CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (70:30, v/v)Flow: 1.0mL/min.Temp.: 25°CDet.: UV @ 238nm

Antiasthmatic Beclomethasone on Ultra C18

0 5 10 15 20 min.

1

2

LC_0120

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Antiasthmatic Beclomethasone on Ultra C18

0 0.5 1 1.5 2 min.

PPeeaakk LLiisstt::1. beclomethasone2. deschlorobeclomethasone

SSaammppllee::Inj.: 10µLConc.: 79.5µg/mL (beclomethasone)Solvent: water:acetonitrile (10:90, v/v)


CCoonnddiittiioonnss::Mobile phase: water:acetonitrile (30:70, v/v)Flow: 1.0mL/min.Temp.: 25°CDet.: UV @ 238nm

1

2

LC_0121

Antibiotic Amoxicillin on Ultra IBD

PPeeaakk LLiisstt::1. amoxicillin

SSaammppllee::Inj.: 5µLConc.: 1.5mg/mLSolvent: acetonitrile:water

(10:90, v/v)





1

0 1 2 3 4 5 6 min.

LC_0095

PPeeaakk LLiisstt::1. cephaloridine




acetate, pH 7.4: acetonitrile (20:80, v/v)

Flow: 0.2mL/min.Temp.: ambientDet.: PE/Sciex API

150 EX Interface: turbo ion spray Ion mode: positiveNebulizer: 8L/hourCurtain gas: 12L/HourIon spray voltage: 4700.0 VTemp.: 350°COrifice: + 10.0 VRing: + 25.0 VSIM monitoring: 416 ± 3 Daltons

Antibiotic Cephaloridine on Ultra IBD (LC/MS)

1

0 1.0 2.0 min.

LC_0124

1

Antibiotic Cephaloridine on Ultra IBD (Normal Phase)


SSaammppllee::Inj.: 5µLConc: 1mg/mLSolvent: acetonitrile:water

(50:50, v/v)



20mM ammonium phosphate, pH 4.0(80:20,v/v)


LC_0101

0 1 2 3 4 5 6 7 8 9 min.

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Antibiotic Cephaloridine on Ultra IBD (Reversed Phase)


SSaammppllee::Inj.: 5µLConc.: 1mg/mLSolvent: acetonitrile:water

(50:50, v/v)


CCoonnddiittiioonnss::Mobile phase: 20mM:acetonitrile

ammonium phosphate, pH 4.0 (20:80,v/v)


LC_0102

1

0 1 2 3 4 5 6 7 8 min.

Antibiotic Cephalexin on Ultra IBD

1

PPeeaakk LLiisstt::1. cephalexin2. cephradine


(10:90, v/v)





2

0 2 4 6 8 10 min.

LC_0094

0 1.0 2.0 min.

1

Antibiotic Cephradine on Ultra IBD (LC/MS)

LC_0125

PPeeaakk LLiisstt::1. cephradine




acetate, pH 7.4: acetonitrile (20:80, v/v)

Flow: 0.2mL/min.Temp.: ambientDet.: PE/Sciex API 150 EX Interface: turbo ion spray Ion mode: positiveNebulizer: 8L/hourCurtain gas: 12L/HourIon spray voltage: 4700.0 VTemp.: 350°COrifice: + 10.0 VRing: + 25.0 VSIM monitoring: 349 ± 3dal.

Antibiotic Penicillin V on Ultra IBD (LC/MS)

1

0 1.0 2.0 min.

LC_0127




acetate pH 7.4: acetonitrile (20:80, v/v)

Flow: 0.2mL/min.Temp.: ambientDet.: PE/Sciex API 150 EX Interface: turbo ion spray Ion mode: positiveNebulizer: 8L/hourCurtain gas: 12L/hourIon spray voltage: 4700.0 vTemp.: 350°COrifice: + 10.0 VRing: + 25.0 VSIM monitoring: 349 ± 3dal.

PPeeaakk LLiisstt::1. penicillin V

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PPeeaakk LLiisstt::1. penicillin V

SSaammppllee::Inj.: 2.5µLConc.: 1.2mg/mLSolvent: acetonitrile:water

(10:90,v/v)





Antibiotic Penicillin V on Ultra IBD

1

0 1 2 3 4 5 6 7 8 min.

LC_0096

Antibiotic Sulfamethoxazole on Allure™ Basix

1

PPeeaakk LLiisstt::1. sulfamethoxazole

SSaammppllee::Inj.: 10µLConc.: 100µg/mLSolvent: water:methanol

(1:1, v/v)




0 2.5 5 7.5 10 12.5 15 17.5 min.

LC_0066

Antibiotic Sulfamethoxazole, Trimethoprim, andNaphthoate on Allure™ C18

1PPeeaakk LLiisstt::

1. trimethoprim2. sulfamethoxazole3. naphthoate

SSaammppllee::Inj.: 10µLConc.: 100µg/mLSolvent: water:methanol

(1:1, v/v)



formate, pH to 3.0with formic acid:methanol (50:50, v/v)

Flow rate: 1.0mL/min.Temp.: ambientDet.: UV @ 220nm

2

0 2 4 6 8 10 12 14 min.LC_0067

3

Antidepressants (Tricyclic) on Allure™ Basix

0 1 2 3 4 5 6 7 8 9 min.

1

2

4

5

7

3

6

LC_0053

PPeeaakk LLiisstt::1. doxepin2. nordoxepin3. amitriptyline4. imipramine5. desimipramine6. nortriptyline7. protriptyline

SSaammppllee::Inj.: 10µLConc.: 100µg/mL eachSolvent: mobile phase



50mM KH2PO4

pH3:methanol (60:25:15, v/v)


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2

0 1 2 3 4 5 6 7 8 9 min.

LC_0112

PPeeaakk LLiisstt::1. desipramine2. trimipramine

SSaammppllee::Inj.: 10µLConc.: 10µg/mLSolvent: water:methanol (1:1, v/v)



formate, pH 3:methanol (25:75, v/v)

Flow: 1.0mL/min. Split ratio: 1:1Column temp.: ambientDet.: PE/Sciex API 150 EXInterface: heated nebulizerIon mode: negative Ion spray voltage: 3000VTemp.: 400°COrifice: -40VRing: -100VAuxiliary gas: 6000cc/min.

Antidepressants (Tricyclic) on Allure™ Basix (LC/MS) Antidepressant Sertraline HCl on Allure™ Basix

1

PPeeaakk LLiisstt::1. toluene (marker)2. sertraline HCl


(7:3, v/v)



20mM ammoniumacetate, pH 4.5(40:60, v/v)


2

0 2 4 6 8 10 12 14 min.

LC_0079

Anti-Diabetic Glyburide (Glybenclamide) on Ultra IBD

1

PPeeaakk LLiisstt::1. unknown2. glyburide

SSaammppllee::Inj.: 1µLConc.: 500mg/mLSolvent: water:methanol:acetone

(1:1:2, v/v)


CCoonnddiittiioonnss::Mobile phase: water with

0.1% (v/v) acetic acid:acetonitrile (95:05, v/v)


2

0 1 2 3 4 5 min.

LC_0069


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0 0.5 1.51 2.52 3 3.5 min.

1

LC_0239

Anti-inflammatory Naproxen on Pinnacle™ DB Cyano

PPeeaakk LLiisstt::1. naproxen


CCoolluummnn:: PPiinnnnaaccllee™ DDBB CCyyaannooCat. #: 9416565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 140Å

CCoonnddiittiioonnss::Mobile phase: 20mM KH2PO4,

pH 3.0:acetonitrile (90:10 v/v)Flow: 1.0mL/min.Temp.: 27°C Det.: UV @ 254nm

0 0.5 1.5 2.51 2 3.5 4.53 4 min.

1

LC_0240

Anti-inflammatory Naproxen on Pinnacle™ DB C8

PPeeaakk LLiisstt::1. naproxen




pH 3.0:acetonitrile (90:10 v/v)Flow: 1.0mL/minTemp.: 27°CDet.: UV @ 254nm

1

LC_0115

PPeeaakk LLiisstt::1. cimetidine


Anti-ulcerative Cimetidine on Ultra IBD (LC/MS)

0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 min.



(20:80, v/v)Flow: 0.2 mL/min.Column temp.: ambientDet.: PE/Sciex API 150 EXInterface: turbo ion sprayIon mode: positive Ion spray voltage: 4800VTemp.: 350°COrifice: 10VRing: 100VAuxiliary gas: 6000cc/min.

Bronchodilator Albuterol on Allure™ Basix

1

PPeeaakk LLiisstt::1. albuterol


(30:70)





0 1 2 3 4 5 min.

LC_0089

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PPeeaakk LLiisstt::1. uracil2. corticosterone3. deoxycorticosterone

SSaammppllee::Inj.: 5µLConc.: 100µg/mL eachSolvent: water:acetonitrile (1:1)




1

2 3

Corticosteroids on Allure™ C18

LC_0054

0 1 2 3 4 min.

PPeeaakk LLiisstt::1. uracil2. triamcinolone3. hydrocortisone4. dexamethasone5. corticosterone6. deoxycorticosterone


Corticosteroids on Ultra C18

0 1 2 3 4 5 6 min.

LC_0108

3

2

1

4

5

6



(30:70, v/v)Flow: 1.0mL/min.Temp.: 30°CDet.: UV @ 220nm

LC_0111 Data Courtesy of Pfizer Inc

PPeeaakk LLiisstt::1. deoxycorticosterone (DCC) acetate2. DCC glucoside3. DCC

CCoolluummnn::Cat. #: 9164552Dimensions: 50 x 2.1mmParticle size: 5µmPore size: 60Å

CCoonnddiittiioonnss::Scan range: 320-520amuFlow rate: 0.4mL/min.LC/MS Interface: positive ion ESI-TOFVoltage: 3800VNozzle temp.: 160VNozzle volt.: 190V

Allure™ C18water:methanol (33:67, v/v)44119911..11 ccppss

Conventional C18water:methanol (40:60, v/v)33332244..00 ccppss

0 1 2 3 4 min.

Corticosteroids on Allure™ C18 vs. Conventional C18 (LC/MS)

12% increase in organic = 26% increase in LC/MS sensitivity

0 1 2 3 4 min.

1

2

3

1

2

3

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0 1 2 3 4 5 6 7 8 min.

12

34

LC_0182

Corticosteroids on Pinnacle II™ Phenyl

PPeeaakk LLiisstt::1. hydrocortisone2. cortisone3. corticosterone4. cortisone acetate

SSaammppllee::Inj.: 5µLConc.: 200µg/mL eachSolvent: methanol



0 2 4 6 8 10 12

1

2

3

LC_0185

Corticosteroids on Pinnacle II™ Cyano (Normal Phase Separation)

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. corticosterone 0.7 2 cortisone acetate 0.43. cortisone 0.4

SSaammppllee::Inj.: 10µLSolvent: ethanol


CCoonnddiittiioonnss::Mobile phase: hexane:ethanol

(90:10, v/v)Flow: 1.0mL/min.Temp.: 30°C Det.: UV @ 254nmPressure: 29 bar

0 1 2 3 4 5 6 7 8 9 min.

1

2

3

LC_0186

Corticosteroids on Pinnacle II™ Cyano (Reversed Phase Separation)

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL))1. cortisone 0.1 2. corticosterone 0.53. cortisone acetate 0.7

SSaammppllee::Inj.: 5.0µLSolvent: methanol



(65:35, v/v)Flow: 1.0mL/min.Temp.: 30°C Det.: UV @ 254nmPressure: 133 bar


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0 1 2 3 4min.

Hormones β-Estradiol, Norethindrone and Ethynyl Estradiol on Ultra C18

PPeeaakk LLiisstt::1. β-estradiol2. ethynyl estradiol3. norethindrone

SSaammppllee::Inj.: 10µLConc.: 333µg/mLSolvent: water:acetonitrile

(20:80, v/v)



formate, pH 3.0: acetonitrile (40:60, v/v)

Flow: 1.0 mL/min.Temp.: 30°CDet.: UV @ 230nm

1

2

3

LC_0122

Hormones β-Estradiol, Norethindrone andEthynyl Estradiol on Ultra C18

PPeeaakk LLiisstt::1. β-estradiol2. norethindrone3. ethynyl estradiol


(80:20, v/v)



formate, pH 3.0: acetonitrile (60:40, v/v)


0 2 4 6 8 10 12 14 min.

1

2

3

LC_0118

PPeeaakk LLiisstt::1. β-estradiol2. norethindrone3. 17-α-ethynyl estradiol


(70:30)





1

2

3

Hormones β-Estradiol, Norethindrone and EthynylEstradiol on Ultra C4

LC_0085

0 1 2 3 4 5 6 min.

Hormones Norethindrone and Ethynyl Estradiol onAllure™ Basix

1

PPeeaakk LLiisstt::1. uracil (marker)2. norethindrone3. ethynyl estradiol




(35:65, v/v)Flow rate: 1.0mL/min.Temp.: 25°CDet.: UV @ 220nm

0 2 4 6 min.

LC_0099

2

3

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LC_0116

Hormones Norgestrel and Ethynyl Estradiol on Ultra C18

0 2.5 5 7.5 10 12.5 15 17.5 20 22.5 min.

1

2

3

PPeeaakk LLiisstt::1. uracil (marker)2. ethynyl estradiol3. norgestrel




(40:60, v/v)Flow: 1.0mL/min.Temp.: 25°CDet.: UV @ 220nm

Hormones Norgestrel and Ethynyl Estradiol on Allure™ Basix

1

PPeeaakk LLiisstt::1. uracil (marker)2. norgestrel3. ethynyl estradiol

0 1 2 3 4 5 6 7 8 9 min.

LC_0100

2

3









(40:60, v/v)Flow: 1.0mL/min.Temp.: 25°C Det.: UV @ 220nm

LC_0117

0 5 10 15 20 25 min.

1

2

3

Hormones Norgestrel and Ethynyl Estradiol on Allure™ C18

Hormones Norgestrel and Ethynyl Estradiolon Allure™ C18

LC_0123

1

2

3

0 1 2 3 4 5 min.




(25:75, v/v)Flow: 1.0mL/min.Temp.: 25°C Det.: UV @ 220nm


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Hormones Norethindrone and MedroxyprogesteroneAcetate on Allure™ Basix

1

PPeeaakk LLiisstt::1. uracil (marker)2. norethindrone3. medroxyprogesterone





0 0.5 1 1.5 2 2.5 3 3.5 4 min.LC_0083

2

3

Hormone Medroxyprogesterone Acetate on Ultra C4

1

PPeeaakk LLiisstt::1. unknown2. medroxyprogesterone

SSaammppllee::Inj.: 1µLConc.: 1mg/mLSolvent: acetonitrile:water

(70:30)





0 1 2 3 4 5 6 7 8 9 min.

LC_0084

2

Hormone Levothyroxine on Ultra PFP

1

PPeeaakk LLiisstt::1. unknown2. levothyroxine

SSaammppllee::Inj.: 20µLConc.: 1.1mg/mLSolvent: water:methanol

(70:30)Add 3 drops of concentrated ammonium hydroxide to each 10mL of solvent. Levothyroxine will not dissolve unless the pH is basic.

CCoolluummnn:: UUllttrraa PPFFPPCat. #: 9176565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100Å


formate, pH 2.5:methanol (60:40, v/v)


2

0 2 4 6 8 10 12 14 16 18 min.

LC_0093

PPeeaakk LLiisstt:: CCoonncc.. ((mmgg//mmLL)) RReett.. TTiimmee ((mmiinn..)) RRRRTT RReess.. IInnjj.. %% RRSSDD ((nn==55))1. fructose 0.4 4.6 0.44 — —2. galactose 6.4 6.1 0.58 — —3. epi-lactose unknown 10.0 0.95 — —4. lactulose 40 10.5 1.00 1.1 0.3%5. lactose 4.8 12.7 1.21 2.9 —

SSaammppllee::Inj.: 20µL resolution standardSolvent: acetonitrile:water (50:50)

LC_0222

Laxative Lactulose Concentrate by USP 25: ResolutionSolution on Pinnacle II™ Amino


CCoonnddiittiioonnss::Mobile phase: A: 10mM sodium phosphate

monobasic in water, pH 4.8B: acetonitrile (22A:78B, v/v)

Flow: 1.3mL/min.Temp.: 40°C Det.: refractive index @ 40°C

497-527_LCAPP.qxd 11/3/2004 2:53 PM Page 525

HP

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526 Pharmaceutical


Mixed Drugs: Analgesic Ibuprofen and DecongestantPseudoephedrine on Allure™ Basix

SSaammppllee::Inj.: 5µL Conc.: 200µg/mLSolvent: mobile phase



acetate, pH 6.9:methanol (75:25, v/v)


PPeeaakk LLiisstt::1. uracil2. ibuprofen3. pseudoephedrine

1

2

3

LC_0098

0 2 4 6 8 10 12 14 min.

Mixed Drugs: Analgesic Acetaminophen, DecongestantPseudoephedrine and Stimulant Caffeine on Allure™ Basix

1

PPeeaakk LLiisstt::1. sample solvent2. caffeine3. acetaminophen4. pseudoephedrine

SSaammppllee::Inj.: 5µL Conc.: 100µg/mLSolvent: mobile phase


CCoonnddiittiioonnss::Mobile phase: 10mM ammonium acetate,

pH 6.9: methanol (75:25, v/v) Flow: 1.0mL/min.Temp.: 25°CDet.: UV @ 210nm

2

3

4

0 2 4 6 8 10 12 min.

LC_0097

Mixed Drugs: Expectorant/Antitussive Guaifenesin andNarcotic Analgesic Codeine on Ultra Phenyl

1

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. codeine 8252. guaifenesin 375

SSaammppllee::Inj.: 5µLSolvent: methanol:water (50:50)

CCoolluummnn:: UUllttrraa PPhheennyyllCat. #: 9105565Dimensions: 150 x 4.6mmParticle size: 5µmPore size: 100Å




0 1 2 3 4 5 6 min.

LC_0088

2PPeeaakk LLiisstt:: CCoonncc.. RReett.. TTiimmee RRRRTT TTaaiilliinngg RReessoolluuttiioonn IInnjj.. %%RRSSDD

((µgg//mmLL)) ((mmiinn..)) ((nn==55))1. acetaminophen 0.76 3.304 1.0 1.1 — 1.12. hydrocodone bitartrate 0.0076 6.664 2.0 1.1 17.0 0.9

SSaammppllee::Inj.: 25µLSample: 500mg acetaminophen with

5mg hydrocodone bitartrate (tablet)Solvent: mobile phase

LC_0212

Mixed Drugs: Hydrocodone Bitartrate/Acetaminophenby USP 25 on Ultra C18


CCoonnddiittiioonnss::Mobile phase: A: 6.8 g potassium

monobasic with 0.2mL of TEA per literB: acetonitrile (85A:15B, v/v)


(hydrocodone bitartrate) and 295nm (acetaminophen).

497-527_LCAPP.qxd 11/3/2004 2:53 PM Page 526

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527Pharmaceutical


PPeeaakk LLiisstt::1. toluene (marker)2. lorazepam3. alprazolam

SSaammppllee::Inj.: 10µLConc.: 500µg/mLSolvent: water:acetonitrile

(7:3, v/v)





1

2

3

Sedatives Alprazolam and Lorazepam on Allure™ Basix

LC_0078

0 1 2 3 4 5 min.

Sedative Cyclobenzaprine on Allure™ Basix

1

PPeeaakk LLiisstt::1. toluene (marker)2. cyclobenzaprine HCl

SSaammppllee::Inj.: 1µLConc.: 1.5µg/mLSolvent: 100% water



formate, pH 3.0acetonitrile (25:75, v/v)


0 1 2 3 4 5 6 min.LC_0087

2

0 1 2 3 4 5 6 7 min.

1

2

3

4

LC_0241

Xanthenes on Pinnacle™ DB C18


CCoolluummnn:: PPiinnnnaaccllee™ DDBB CC1188Cat. #: 9414565Dimensions: 150 x 4.6 mmParticle size: 5µmPore size: 140Å


pH 3.0:acetonitrile (90:10 v/v)


PPeeaakk LLiisstt:: CCoonncc.. ((µµgg//mmLL))1. theobromine 2252. theophylline 4183. β-hydroxyethyltheophylline 4184. caffeine 400

1

2

34

86420 min.

PPeeaakk LLiisstt:: CCoonncc.. ((µµgg//mmLL))1. theobromine 2002. theophylline 3903. β-hydroxytheophylline 3904. caffeine 390

LC_0288

Xanthenes on Pinnacle™ DB C18SSaammppllee::Inj.: 2µL Solvent: water:methanol

(75:25 v/v)



pH 3:methanol (80:20 v/v)


LC_0290

Xanthenes on Pinnacle II™ C18

PPeeaakk LLiisstt:: CCoonncc.. ((µgg//mmLL))1. theobromine 2002. theophylline 3903. β-hydroxytheophylline 3904. caffeine 390



CCoonnddiittiioonnss::Mobile phase: 20mM KH2PO4 pH 3:

methanol (80:20 v/v)Flow: 1.0mL/min.Temp.: ambientDet.: UV @ 254nm

497-527_LCAPP.qxd 11/3/2004 2:53 PM Page 527

GC ApplicationsOur library of chromatograms will save you time when you are researching a new application or attempting to improve on current

results. This selection includes representative GC chromatograms for environmental contaminants, petrochemicals, industrial

chemicals, biochemicals, pharmaceuticals, nutraceuticals, foods, flavors & fragrances, and forensics. To see our newest

chromatograms, subscribe to the Restek Advantage, and regularly visit our web site at www.restek.com.

Chris EnglishInnovations Group Leader

528-544_GCAPP.qxd 11/3/2004 5:02 PM Page 528

www.restek.com

Volatiles


GC

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L529

Choosing a Volatiles GC Column for PID/ELCD

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. 1,1-dichloroethene8. Freon ®1139. methylene chloride

10. trans-1,2-dichloroethene11. methyl tert-butyl ether12. tert-butyl alcohol13. 1,1-dichloroethane14. cis-1,2-dichloroethene15. 2,2-dichloropropane16. bromochloromethane17. chloroform18. carbon tetrachloride19. 1,1,1-trichloroethane20. 1,1-dichloropropene21. benzene22. 1,2-dichloroethane23. fluorobenzene24. trichloroethene25. dibromomethane26. 1,2-dichloropropane27. bromodichloromethane28. 2-chloroethyl vinyl ether29. cis-1,3-dichloropropene30. toluene31. tetrachloroethene32. trans-1,3-dichloropropene33. 2-bromo-1-chloropropane34. 1,1,2-trichloroethane35. dibromochloromethane

36. 1,3-dichloropropane37. 1,2-dibromoethane38. chlorobenzene39. ethyl benzene40. 1,1,1,2-tetrachloroethane41. m-xylene42. p-xylene43. 1-chloro-2-fluorobenzene44. o-xylene45. styrene46. bromoform47. isopropyl benzene48. bromobenzene49. n-propylbenzene50. 1,4-dichlorobutane51. 1,1,2,2-tetrachloroethane52. 2-chlorotoluene53. 1,3,5-trimethylbenzene54. 1,2,3-trichloropropane55. 4-chlorotoluene56. tert-butylbenzene57. 1,2,4-trimethylbenzene58. sec-butylbenzene59. p-isopropyl toluene60. 1,3-dichlorobenzene61. 1,4-dichlorobenzene62. n-butylbenzene63. 1,2-dichlorobenzene64. 4-bromo-1-chlorobenzene65. 1,2-bromo-3-chloropropane66. hexachlorobutadiene67. 1,2,4-trichlorobenzene68. naphthalene69. 1,2,3-trichlorobenzene

Conditions for Rtx®-VGC, Rtx®-502.2, Rtx®-Volatiles, Rtx®-VRX and Rtx®-1:optimum conditions on 75 m, 0.45mm ID, 2.55µm columns.

Conditions for all other columns: Column: 60m, 0.53mm ID, 3.0µm column flow 10mL/min., GC Program: 35°C (hold 9 min.) to 220°C @ 11 min. (hold 10 min.)

Analytes identified using HP5971a mass selective detector with splitless injec-tion or using Finnigan PID/ELCD

Please see chromatograms on pages 530–544 for more details, or call techni-cal service at 800-356-1688 (ext. 4), or your Restek representative.

1 Can be resolved under different conditions. See application showing method 601/602 with the Rtx®-VGC column on www.restek.com/chromatograms(search for chromatogram number GC_EV00420).2 pages listed include GC/MS analyses.

Coelutions by Peak #’s Suggested Poor Choice for Recomended Restek (Coelutions by PID/ELCD Close Pairs Confirmation Confirmation Thick Phase High Temp. Page # for Phase are indicated in BOLD) by PID/ELCD Column Column Stable Temp. for VOA Work Advantages Chromatogram2

Rtx-VGC 28/29 1, 53/54 7/8, 32/33 Rtx-502.2, Rtx-VRX, Rtx-VMS, Rtx-1 Rtx-DX1 260 230 fast runtime 530, 532

Rtx-502.2 14/15, 33/34, 39/40 4/5, 44/45, 56/57, Rtx-VGC, Rtx-VRX, Rtx-Volatiles, 533, 534,52/55, 64/65 Rtx-1 Vocol, Rtx-35, Rtx-20 270 240 low bleed 535, 544

Rtx-Volatiles 14/15, 21/22, 38/40, 56/57, 68/69 Rtx-VGC, Rtx-1, Rtx-502.2, Vocol,44/45, 53/55 Rtx-624 Rtx-20, Rtx-35 270 240 low bleed no

Rtx-624 7/8, 10/11, 52/53, 32/33, 44/45, Rtx-VGC, 31/34, 53/55, 59/60 51/54 Rtx-502.2 Rtx-1701 280 240 no

Rtx-VRX 11/13, 39/43, 46/50, 40/44 8/9, 15/17, 24/27, Rtx- VGC, 58/60 Rtx-502.2 Rtx-1 260 230 531, 536

Rtx-1 9/12, 15/17, 25/26, 24/27, 33/36, 38/40, 40/44, 45/50, 56/57 7/12, 49/55 Rtx-502 Rtx-VRX 320 260 no

Rtx-1701 9/10, 18/19, 16/20, 50/53, 51/55, 54/56 5/6, 29/30, 32/33 Rtx-502.2 Rtx-624 270 240 no

Rtx-200 2/3, 5/6, 11/12, 14/16, 22/24, 28/35, m/p xylene 32/33/37, 43/44, 50/55/56, 57/58 13/17,36/37 Rtx-VGC 320 240 separation no

Rtx-35 4/5, 16/19, 18/20, 21/22, 34/31, Rtx-VGC, Rtx-502.2, 39/38/41/42/40, 46/51/49, 53/54, Rtx-624 Rtx-Volatiles, 48/52/55, 61/62, 66/67 2/3, Rtx-20 270 240 no

Rtx-50 4/5/6, 8/7/12, 25/28, 32/33,37/41/42, 38/40, 45/47, 46/54/52, 2/3, 20/18, 31/32, Rtx-VGC, 56/55/48, 57/58 39/41/42 Rtx-624 Rtx-35 280 240 no

Rtx-DX1 4/5, 9/10, 25/27, 38/39, 47/50, 27/28, 32/36/31, (custom) 49/46/48, 52/54, 53/55 65/67 Rtx-502.2 Rtx-VGC 220 200 no

Compounds listed in US EPA Methods 502.2, 8021, 8010, 8020, 601 & 602, plus commonly added compounds.

m/p xylene coelute on all phases listed above except Rtx®-200 in 60m, 0.25mm ID, 1.0µm under optimized conditions. See 8260 application, using the Rtx®-200 column, www.restek.com/chromatograms (search for chromatogram number GC_EV00429).

Volatile Analytes:

for more infoFor GC/MS analyses, see Rtx®-VMS applications on pages553377––554433.

528-544_GCAPP.qxd 11/3/2004 5:02 PM Page 529

Volatiles


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530

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. 1,1-dichloroethene8. Freon® 1139. allyl chloride

10. methylene chloride11. trans-1,2-dichloroethene12. methyl tert-butyl ether13. 1,1-dichloroethane14. cis-1,2-dichloroethene

15. 2,2-dichloropropane16. bromochloromethane17. chloroform18. carbon tetrachloride19. 1,1,1-trichloroethane20. 1,1-dichloropropene21. benzene22. 1,2-dichloroethane23. fluorobenzene (SS)24. trichloroethene25. dibromomethane26. 1,2-dichloropropane27. bromodichloromethane28. 1-bromo-2-chloroethane (SS)

29. cis-1,3-dichloropropene30. toluene31. tetrachloroethene32. trans-1,3-dichloropropene33. 1,1,2-trichloroethane34. dibromochloromethane35. 1,3-dichloropropane36. 1,2-dibromoethane37. 1-chloro-3-fluorobenzene (SS)38. chlorobenzene39. ethylbenzene40. 1,1,1,2-tetrachloroethane41. m-xylene42. p-xylene

43. o-xylene44. styrene45. bromoform46. isopropylbenzene47. bromobenzene48. n-propylbenzene49. 1,1,2,2-tetrachloroethane50. 2-chlorotoluene51. 1,2,3-trichloropropane52. 1,3,5-trimethylbenzene53. 4-chlorotoluene54. tert-butylbenzene55. 1,2,4-trimethylbenzene56. sec-butylbenzene

57. p-isopropyltoluene58. 1,3-dichlorobenzene59. 1,4-dichlorobenzene60. n-butylbenzene61. 1,2-dichlorobenzene62. 1,2-dibromo-3-chloropropane63. hexachlorobutadiene64. 1,2,4-trichlorobenzene65. naphthalene66. 1,2,3-trichlorobenzene

20ppb in 5mL of RO water.Column: 75m, 0.45mm ID, 2.55µm Rtx®-VGC (cat.# 19409)Confirmation column: 75m, 0.45mm ID, 2.55µm Rtx®-VRX (cat.# 19309)Concentrator: Tekmar LSC-3000 Purge and TrapTrap: Vocarb™ 3000 Purge: 11 min. @ 40mL/min. Dry purge: 1 min. @ 40mL/min. (MCS by-passed with

Silcosteel® tubing [cat.# 21035])Desorb preheat: 245°C Desorb: 250°C for 2 min. Bake: 260°C for 8 min.Interface: directTransfer line: 0.32mm ID Siltek™ tubing

GC: Finnigan 9001Oven temp.: 35°C (hold 4 min.) to 75°C @ 3°C/min. (hold 2 min.)

to 175°C @ 21°C/min. to 205°C @35°C/min. (hold 5 min.)

Carrier gas: helium 11mL/min., constant pressureAdjust dichlorodifluoromethane to a retention time of 2.28 min. @ 35°C on the Rtx®-VGC column.

Detectors: µGold Tandem PID/HALL 2000PID: makeup 7mL/min., purge 7mL/min., set @ 0.35mV, base temp. 200°C.ELCD Hall 2000: RxnGas 25mL/min., RxnTemp. 940°C, propanol flow 470µL/min.

Volatile OrganicsUS EPA Method 8021Rtx®-VGCPrimary column, dual-column analysis. Polymer specially designed for volatiles analysis by PID/ELCD. Confirmation analysis shown on page 531.

Rtx®-VGC75m, 0.45mm ID, 2.55µm (cat.# 19409)

PID

min. 5 10 15 20 25

37

11

12

14

20

21

2324

29

30

3132

37

63

64

65

66

37

3839

41,42

43

44

46

47

48

50

52

53

54

55

5657

5958

6061

22 23 24 25 26

• 35°C starting temperature forbetter resolution of early-elutingcompounds.

• Excellent resolution of trihalomethanes.

ELCD

47

49

50

51

53

24 24.5 25

7

8

9

10

11

13

4

5

6

1 2

3

20

22

23

24

25

2627

28

29

3132

3314

15

16

17

18

19

34

35

36

37

38

40

45

58

59

62

61

63

64

66

Acknowledgement: Finnigan 9001 GC, µGold Tandem Photoionization/HALL® 2000 Electrolytic Conductivity Detector provided courtesy of Thermo Finnigan GC & GC/MS Division, 2215 GrandAvenue Pkwy, Austin, Texas 78728

GC_EV00416min. 5 10 15 20 25

Freon® 113 included (peak 8)

ELCDPID

restekinnovation!

528-544_GCAPP.qxd 11/3/2004 5:02 PM Page 530

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min. 5 10 15 20 25 30

Volatile OrganicsUS EPA Method 8021Rtx®-VRXExcellent confirmation column to Rtx®-VGC. Primary analysis shown on page 530.

min. 5 10 15 20 25

PID

PID ELCD

Rtx®-VRX75m, 0.45mm ID, 2.55µm (cat.# 19309)

3

7

9

11

12 1420

21

23 24

29

32

3031

37

38

39

41 42

44

43

64

65

66

63

1

2

3

4

5

6

7

10

8,9

11

13

14

16

17

15

22

19

20

18

25

26

24,27

28

2932

33

35

34

36

31

37

40

3845

49

51

47

5053

58

59

61

62

64

63

66

31

37

3839

41 42

44

4346

47

48

50

53

52

54

55

56,58

59

5761

60

20.0 22.5 25.0

Acknowledgement: Finnigan 9001 GC, µGold Tandem Photoionization/HALL® 2000 Electrolytic Conductivity Detector provided courtesy of Thermo Finnigan GC & GC/MS Division,2215 Grand Avenue Pkwy, Austin, Texas 78728

• 35°C starting temperaturefor better resolution of early-eluting compounds.

Freon® 113 included(peak 8)

23

GC_EV00417

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. 1,1-dichloroethene8. Freon® 1139. allyl chloride

10. methylene chloride11. trans-1,2-dichloroethene12. methyl tert-butyl ether13. 1,1-dichloroethane14. cis-1,2-dichloroethene15. 2,2-dichloropropane16. bromochloromethane17. chloroform

18. carbon tetrachloride19. 1,1,1-trichloroethane20. 1,1-dichloropropene21. benzene22. 1,2-dichloroethane23. fluorobenzene (SS)24. trichloroethene25. dibromomethane26. 1,2-dichloropropane27. bromodichloromethane28. 1-bromo-2-chloroethane (SS)29. cis-1,3-dichloropropene30. toluene31. tetrachloroethene32. trans-1,3-dichloropropene33. 1,1,2-trichloroethane34. dibromochloromethane

35. 1,3-dichloropropane36. 1,2-dibromoethane37. 1-chloro-3-fluorobenzene (SS)38. chlorobenzene39. ethylbenzene40. 1,1,1,2-tetrachloroethane41. m-xylene42. p-xylene43. o-xylene44. styrene45. bromoform46. isopropylbenzene47. bromobenzene48. n-propylbenzene49. 1,1,2,2-tetrachloroethane50. 2-chlorotoluene51. 1,2,3-trichloropropane

52. 1,3,5-trimethylbenzene53. 4-chlorotoluene54. tert-butylbenzene55. 1,2,4-trimethylbenzene56. sec-butylbenzene57. p-isopropyltoluene58. 1,3-dichlorobenzene59. 1,4-dichlorobenzene60. n-butylbenzene61. 1,2-dichlorobenzene62. 1,2-dibromo-3-chloropropane63. hexachlorobutadiene64. 1,2,4-trichlorobenzene65. naphthalene66. 1,2,3-trichlorobenzene

20ppb in 5mL of RO water.Column: 75m, 0.45mm ID, 2.55µm Rtx®-VRX (cat.# 19309)Primary column: 75m, 0.45mm ID, 2.55µm Rtx®-VGC (cat.# 19409)Concentrator: Tekmar LSC-3000 Purge and TrapTrap: Vocarb™ 3000 Purge: 11 min. @ 40mL/min. Dry purge: 1 min. @ 40mL/min. (MCS by-passed with

Silcosteel® tubing [cat.# 21035])Desorb preheat: 245°C Desorb: 250°C for 2 min. Bake: 260°C for 8 min.Interface: directTransfer line: 0.32mm ID Siltek™ tubing

GC: Finnigan 9001Oven temp.: 35°C (hold 4 min.) to 75°C @ 3°C/min. (hold 2 min.)

to 175°C @ 21°C/min. to 205°C @ 35°C/min. (hold 5 min.) Carrier: helium 11mL/min., constant pressure

Adjust dichlorodifluoromethane to a retention time of 2.28 min. @ 35°C on the Rtx®-VGC column.

Detectors: µGold Tandem PID/HALL 2000PID: makeup 7mL/min., purge 7mL/min., set @ 0.35mV, base temp. 200°C.ELCD Hall 2000: RxnGas 25mL/min., RxnTemp. 940°C, propanol flow 470µL/min.

restekinnovation!

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Volatile OrganicsUS EPA Method 8021B Expanded ListRtx®-VGCPrimary column, dual-column analysis. Confirmation analysisshown on page 533.

Column: 75m, 0.45mm ID, 2.55µm Rtx®-VGC (cat.# 19409)Confirmation column: 75m, 0.45mm ID, 2.55µm Rtx®-502.2 (cat.# 10986) Conc.: 10ppb in 5mL of RO waterConcentrator: Tekmar LSC-3000 Purge and TrapTrap: Vocarb 3000Purge: 11 min. @ 40mL/min. Dry purge: 1 min. @ 40mL/min. (MCS bypassed using

Silcosteel® tubing)Desorb preheat: 245°C, Flow 10mL/min. Desorb: 250°C for 2 min.Bake: 260°C for 8 min.Interface: direct using 0.32mm ID Siltek™ transfer lineGC: Finnigan 9001Carrier gas: helium @ ~10mL/min. constant pressure


Oven temp.: 45°C (hold 4 min.) to 70°C @ 2°C/min. to 210°C @ 20°C/min. (hold 10 min.)

Detectors: µGold Tandem PID/HALL 2000PID: makeup 7mL/min., purge 7mL/min., set @0.35mV base temp. 200°C.Hall 2000: RxnGas 25mL/min., RxnTemp. 940°C,propanol flow 470µL/min.

PID

ELCD

PID ELCD

Rtx®-VGC75m, 0.45mm ID, 2.55µm (cat.# 19409)

3

9 13

710

14

15

16

1824

25

2728

33,34

35

3637

70

72

71

73

74

75 76

1

2

3

4

5

6

7,8

9

11

12

13

16 17

18

20

19

21

22

23

24

26

27

28

29

30 31

32

33,34

36

37

38

39

40

4142

44

47

50

60

64

6568

69

70

74

71

72

4243

45 46

47

48

49

51

52

5355

57

58

59

61

6263

6465

66,67

68

52

54

55

56

58

min. 5 10 15 20 25

20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0

22.1 22.2 22.3 22.4 22.5 22.6 22.7 22.8

66

GC_EV00421min. 5 10 15 20 25

Acknowledgement: Finnigan 9001 GC, µGold Tandem Photoionization/HALL® 2000 Electrolytic Conductivity Detector provided courtesy of Thermo Finnigan GC & GC/MS Division, 2215 GrandAvenue Pkwy, Austin, Texas 78728

72 target compounds

please notesee ppaaggee 553333 for peak identifications.

528-544_GCAPP.qxd 11/3/2004 5:02 PM Page 532

Volatiles


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Volatile OrganicsUS EPA Method 8021B Expanded ListRtx®-502.2Confirmation column to Rtx®-VGC. Primary analysis shown on page 532.

PID ELCD3

7,9

15

10,11

14

13

16

1824

25

2728

3334

35

37

36

70

72

73

71

74

75 76

1

2

3

4

5

6

8

7,9

10,11

12

13

1716

19,1821

2023

24

22

26

27

28

30 31

29

33

32

34

37

38

36,40

39

44

41

42

47

50

54

56

55

58

52

60

64

6568

66

69

70

72

71

74

min. 5 10 15 20 25 30

PID

4243

47

45

48,49

46

51

53

52

55,57

5859,61

6263

64 65

66

6768

min. 22.5 25.0

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. 1,1-dichloroethene8. Freon® 1139. chloromethyl methyl ether

10. iodomethane (40ppb)11. allyl chloride12. methylene chloride13. trans-1,2-dichloroethene14. methyl tert-butyl ether15. tert-butyl alcohol (40ppb)16. chloroprene17. 1,1-dichloroethane18. cis-1,2-dichloroethene19. 2,2-dichloropropane

20. bromochloromethane21. chloroform22. carbon tetrachloride23. 1,1,1-trichloroethane24. 1,1-dichloropropene25. benzene26. 1,2-dichloroethane27. fluorobenzene (SS)28. trichloroethene29. dibromomethane30. 1,2-dichloropropane31. bromodichloromethane32. 1-bromo-2-chloroethane (SS)33. 2-chloroethyl vinyl ether34. cis-1,3-dichloropropene35. toluene36. tetrachloroethene37. trans-1,3-dichloropropene38. 1,1,2-trichloroethane

39. dibromochloromethane40. 1,3-dichloropropane41. 1,2-dibromoethane42. chlorobenzene43. ethylbenzene44. 1,1,1,2-tetrachloroethane45. m-xylene46. p-xylene47. 1-chloro-2-fluorobenzene (SS)48. o-xylene49. styrene50. bromoform51. isopropylbenzene52. bromobenzene53. n-propylbenzene54. 1,1,2,2-tetrachloroethane55. 2-chlorotoluene56. 1,2,3-trichloropropane57. 1,3,5-trimethylbenzene

58. 4-chlorotoluene59. tert-butylbenzene60. pentachloroethane61. 1,2,4-trimethylbenzene62. sec-butylbenzene63. p-isopropyltoluene64. 1,3-dichlorobenzene65. 1,4-dichlorobenzene66. benzyl chloride67. n-butylbenzene68. 1,2-dichlorobenzene69. 1,2-dibromo-3-chloropropane70. 2-bromo-1-chlorobenzene (SS)71. hexachlorobutadiene72. 1,2,4-trichlorobenzene73. naphthalene74. 1,2,3-trichlorobenzene75. 2-methylnaphthalene (40ppb)76. 1-methylnaphthalene (40ppb)

Column: 75m, 0.45mm ID, 2.55µm Rtx®-502.2 (cat.# 10986)Primary column: 75m, 0.45mm ID, 2.55µm Rtx®-VGC (cat.# 19409) Conc.: 10 ppb in 5mL of RO waterConcentrator: Tekmar LSC-3000 Purge and TrapTrap: Vocarb 3000Purge: 11 min. @ 40mL/min. Dry purge: 1 min. @ 40mL/min. (MCS bypassed using

Silcosteel® tubing)Desorb preheat: 245°C, Flow 10mL/min. Desorb: 250°C for 2 min.Bake: 260°C for 8 min.Interface: direct using 0.32 mm ID Siltek™ transfer lineGC: Finnigan 9001Carrier gas: helium @ ~10mL/min. constant pressure



Detectors: µGold Tandem PID/HALL 2000PID: makeup 7mL/min., purge 7mL/min., set @0.35mV base temp. 200°C.Hall 2000: RxnGas 25mL/min., RxnTemp. 940°C,propanol flow 470µL/min.

Rtx®-502.275m, 0.45mm ID, 2.55µm (cat.# 10986)

GC_EV00422

Acknowledgement: Finnigan 9001 GC, µGoldTandem Photoionization/HALL® 2000 ElectrolyticConductivity Detector provided courtesy of ThermoFinnigan GC & GC/MS Division, 2215 GrandAvenue Pkwy, Austin, Texas 78728

min. 5 10 15 20 25 30

restekinnovation!

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Column: 75m, 0.45mm ID, 2.55µm Rtx®-502.2 (cat.# 10986).Conc.: 20ppb in 5mL of RO water (unless otherwise noted, peak 14).Inj.: a combination of the following reference materials was used:

502.2 Cal2000 MegaMix™ (cat.# 30431)502.2 Calibration Mix #1A (cat.# 30439)502.2 Internal Standard Mix #2 (cat.# 30041)1-chloro-2-fluorobenzene (cat.# 30040)4-bromochlorobenzene (cat.# 30230)2-chlorethyl vinyl ether (cat.# 30265)1,4-dichlorobutane (cat.# 30227)MTBE (cat.# 30402)and custom mixtures of Freon® 113, allyl chloride, chloroprene, and benzyl choride.

Concentrator: Tekmar LSC-3000 Purge and TrapTrap: Vocarb 3000Purge: 11 min. @ 40mL/min.Dry purge: 1 min. @ 40mL/min. (MCS off)Desorb preheat: 245°CDesorb: 250°C for 2 min.Bake: 260°C for 8 min.

GC: Finnigan 9001Carrier gas: helium, 9mL/min. constant pressureOven temp.: 35°C (hold 6 min.) to 115°C @ 11°C/min. (hold 7

min.) to 130°C @ 7°C/min. (no hold), to 220°C @9.2°C/min. (hold 4 min.)

Detectors: µGold Tandem PID/HALL 2000PID: makeup 7mL/min., purge 7mL/min. set @0.35mV, base temp. 200°CHall 2000: Rxn gas 25mL/min., Rxn temp. 940°Cpropanol flow 470µL/min.

12

1

2

3

4

5

6

7

8

9

10

37

24

13

14

15

1617

18

19

20

21

22

39

36

25

2627

28

29

30

38

32

40

44

35

66

47

49

50

51

53

54

56

61

62

63

65

67

68

69

71

ELCD

33,34

Volatile OrganicsUS EPA Method 502.2Rtx®-502.2

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. Freon® 1138. 1,1-dichloroethene9. allyl chloride

10. methylene chloride11. methyl tert-butyl ether12. trans-1,2-dichloroethene13. 1,1-dichloroethane14. chloroproprene (40ppb)15. 2,2-dichloropropane16. cis-1,2-dichloroethene17. chloroform18. bromochloromethane19. 1,1,1-trichloroethane20. 1,1-dichloropropene21. carbon tetrachloride22. 1,2-dichloroethane23. benzene24. fluorobenzene (SS)25. trichloroethene26. 1,2-dichloropropane27. bromodichloromethane28. dibromomethane29. 2-chloroethyl vinyl ether30. cis-1,3-dichloropropene31. toluene32. trans-1,3-dichloropropene33. 1,1,2-trichloroethane34. 2-bromo-1-chloropropane (IS)35. 1,3-dichloropropane36. tetrachloroethene

37. dibromochloromethane38. 1,2-dibromoethane39. chlorobenzene40. 1,1,1,2-tetrachloroethane41. ethyl benzene42. m-xylene43. p-xylene44. 1-chloro-2-fluorobenzene (IS)45. o-xylene46. styrene47. bromoform48. isopropyl benzene49. 1,4-dichlorobutane (SS)50. 1,1,2,2-tetrachloroethane51. 1,2,3-trichloropropane52. n-propylbenzene53. bromobenzene54. 2-chlorotoluene55. 1,3,5-trimethylbenzene56. 4-chlorotoluene57. tert-butylbenzene58. 1,2,4-trimethylbenzene59. sec-butylbenzene60. p-isopropyltoluene61. 1,3-dichlorobenzene62. 1,4-dichlorobenzene63. benzyl chloride64. n-butylbenzene65. 1,2-dichlorobenzene66. 1,2-bromo-3-chloropropane67. 4-bromo-1-chlorobenzene (SS)68. 1,2,4-trichlorobenzene69. hexachlorobutadiene70. naphthalene71. 1,2,3-trichlorobenzene

PID

min. 5 10 15 20 25 30

4416

3

20

23

2425

8

30

31

11

12

32

14

36

3941

60

42/43

45

46

48

52

53

54/55

56

57

58

59

6162

63

64

65

67

68

6970

71

Acknowledgement: Finnigan 9001 GC, µGold Tandem Photoionization/HALL® 2000 ElectrolyticConductivity Detector provided courtesy of Thermo Finnigan GC & GC/MS Division, 2215 GrandAvenue Pkwy, Austin, Texas 78728

GC_EV00005

min. 5 10 15 20 25 30

Rtx®-502.275m, 0.45mm ID, 2.55µm (cat.# 10986)

for more infoA confirmational analysis can be found onwwwwww..rreesstteekk..ccoomm//cchhrroommaattooggrraammss

Search for chromatogram numberGGCC__EEVV0000000066

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VIR

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NTA

L535

min. 5 10 15 20

3

7 10

9

16

1819

22

23

24

26

25

29

30

31

3536

37

38

12

3

4

5

6

7

89

11

13

12

15

14

17

18

19

2021

22

23 26

27

25

28

2931

32

33

34

35

36

37

38

PID ELCD

Column: 75m, 0.45mm ID, 2.55µm Rtx®-502.2 (cat.# 10986)Primary column: 75m, 0.45mm ID, 2.55µm Rtx®-VGC (cat.# 19409)Conc.: 10ppb in 5mL of RO water (see below)Concentrator: Tekmar LSC3100 Purge and TrapTrap: Vocarb 3000Purge: 11 min. @ 40mL/min.Dry purge: 1 min. @ 40mL/min.Desorb preheat: 245°CDesorb: 250°C for 2 min.Bake: 260°C for 8 min.Interface: direct connection from concentrator to columnTransfer line: Silcosteel® TubingGas chromatograph: Finnigan 9001Carrier gas: helium @ ~10mL/min. constant pressureDead time: 2.04 min.


Oven temp.: 40°C (hold 2 min.) to 58°C @ 4°C/min. to 90°C @ 10°C/min. (hold 5 min.) to 220°C @40°C/min. (hold 5 min.)

Detectors: µGold Tandem PID/Hall 2000PID: makeup 7mL/min., purge 7mL/min., set @0.35mV base temp.: 200°C.Hall 2000: RxnGas 25mL/min., Rxn Temp. 940°C,propanol flow 470µL/min.

RReeffeerreennccee SSttaannddaarrdd ccaatt..##502.2 mix#1 30042624 cal mix #2 30021624 cal mix #3 30022MTBE 304021,4-dichlorobutane 30227fluorobenzene 300301-chloro-2-fluorobenzene 300404-bromo-1-chlorobenzene 30230bromochloromethane 30225

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. 1,1-dichloroethene8. methylene chloride9. trans-1,2-dichloroethene

10. methyl tert-butyl ether11. 1,1-dichloroethane12. bromochloromethane13. chloroform14. carbon tetrachloride15 1,1,1-trichloroethane16. benzene17. 1,2-dichloroethane18. fluorobenzene19. trichloroethene20. 1,2-dichloropropane

21. bromodichloromethane22. 2-chloroethyl vinyl ether23. cis-1,3-dichloropropene24. toluene25. tetrachloroethene26. trans-1,3-dichloropropene27. 1,1,2-trichloroethane28. dibromochloromethane29. chlorobenzene30. ethylbenzene31. 1-chloro-2-fluorobenzene32. bromoform33. 1,4-dichlorobutane34. 1,1,2,2-tetrachloroethane35. 1,3-dichlorobenzene36. 1,4-dichlorobenzene37. 1,2-dichlorobenzene38. 4-bromo-1-chlorobenzene

Volatile OrganicsUS EPA Method 601/602Rtx®-502.2Confirmation column to Rtx®-VGC.

min. 5 10 15 20

GC_EV00540

Acknowledgement: Finnigan 9001 GC, µGold Tandem Photoionization/HALL® 2000Electrolytic Conductivity Detector provided courtesy of Thermo Finnigan GC & GC/MSDivision, 2215 Grand Avenue Pkwy, Austin, Texas 78728

Rtx®-502.275m, 0.45mm ID, 2.55µm (cat.# 10986)

Best resolution for Method 601 / 602

for more infoThe primary analysis (Rtx®-VGC) can be found onwwwwww..rreesstteekk..ccoomm//cchhrroommaattooggrraammss

Search for chromatogram number GGCC__EEVV0000442200

528-544_GCAPP.qxd 11/3/2004 5:02 PM Page 535

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Volatile OrganicsUS EPA Method 8021Rtx®-VRX

3

PID

min. 5 10 15 20 25

7

10

1113

19

21

2225

27 28

29

31

35

6364

6566

37

38

39/40

42

45

47

48

49

50

51

52

53

54

55 56

57

5859

60

62

22.5 25.0 27.5

min. 5 10 15 20 25

12

3

4

5

6

7

8

9

10

1213

14

15

1617

1819

20

22

23

24

25

26

27

28

30

29

32

33

34

35 36

37

4143

44

46

48

5051

56

57

59

62

61

63

65

66

ELCD

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. 1,1-dichloroethene8. methylene chloride9. Freon®113

10. trans-1,2-dichloroethene11. methyl tert-butyl ether12. 1,1-dichloroethane13. cis-1,2-dichloroethene14. bromochloromethane15. chloroform16. 2,2-dichloropropane17. 1,2-dichloroethane18. 1,1,1-trichloroethane19. 1,1-dichloropropene20. carbon tetrachloride21. benzene22. fluorobenzene (SS)23. dibromomethane24. 1,2-dichloropropane25. trichloroethene26. bromodichloromethane27. 2-chloroethyl vinyl ether28. cis-1,3-dichloropropene29. trans-1,3-dichloropropene30. 1,1,2-trichloroethane31. toluene32. 1,3-dichloropropane33. dibromochloromethane34. 1,2-dibromoethane35. tetrachloroethene36. 1,1,1,2-tetrachloroethane37. chlorobenzene38. ethyl benzene39. m-xylene40. p-xylene41. bromoform42. styrene43. 1,4-dichlorobutane (SS)44. 1,1,2,2-tetrachloroethane45. o-xylene46. 1,2,3-trichloropropane47. isopropyl benzene48. bromobenzene49. n-propylbenzene50. 2-chlorotoluene51. 4-chlorotoluene52. 1,3,5-trimethylbenzene53. tert-butylbenzene54. 1,2,4-trimethylbenzene55. sec-butylbenzene56. 1,3-dichlorobenzene57. 1,4-dichlorobenzene58. p-isopropyltoluene59. 1,2-dichlorobenzene60. n-butylbenzene61. 1,2-bromo-3-chloropropane62. 4-bromo-1-chlorobenzene (SS)63. 1,2,4-trichlorobenzene64. naphthalene65. hexachlorobutadiene66. 1,2,3-trichlorobenzene

Acknowledgement: Finnigan 9001 GC, µGold Tandem Photoionization/HALL® 2000 Electrolytic Conductivity Detector provided courtesy of Thermo Finnigan GC & GC/MSDivision, 2215 Grand Avenue Pkwy, Austin, Texas 78728

75m, 0.45mm ID, 2.55µm Rtx®-VRX (cat.# 19309)Conc.: 20ppb in 5mL of RO water (see below)Concentrator: Tekmar LSC-3000 Purge and TrapTrap: Vocarb 3000Purge: 11 min. @ 40mL/min. Dry purge: 1 min. @ 40mL/min. (MCS by-

passed with Silcosteel® tubing)Desorb preheat: 245°CDesorb: 250°C for 2 min. Bake: 260°C for 8 min.Interface: directTransfer line: 0.32mm ID Siltek™ tubing GC: Finnigan 9001GC program: 35°C (hold 12 min.) to 60°C

@ 5°C/min. (hold 1 min.) to220°C @ 17°C/min. (hold 3 min.)

Carrier gas: helium 9mL/min.Detectors: µGold Tandem PID/HALL 2000

PID: makeup 7mL/min., purge 7mL/min, set @ 0.35mV base temp. 200°C.ELCD Hall 2000: RxnGas 25mL/min., RxnTemp.: 940°C, propanol flow 470µL/min.

PID

RReeffeerreennccee SSttaannddaarrdd ccaatt..##502.2 Cal2000 MegaMix™ 30431502.2 Calibration Mix#1A 304394-bromochlorobenzene 302302-chloroethyl vinyl ether 302651,4-dichlorobutane 30227fluorobenzene 30030methyl-tert-butyl-ether 30402Freon® 113 custom

GC_EV00001

• Good choice for waste water analysis.• See page 530 for recommended

drinking water analysis.

528-544_GCAPP.qxd 11/3/2004 5:02 PM Page 536

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NTA

L537

Volatile OrganicsUS EPA Method OLC 03.2Rtx®-VMS

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. 1,1-dichloroethene8. carbon disulfide9. Freon® 113

10. methylene chloride11. acetone12. trans-1,2-dichloroethene13. methyl acetate14. methyl tert-butyl ether

15. 1,1-dichloroethane16. cis-1,2-dichloroethene17. cyclohexane18. bromochloromethane19. chloroform20. carbon tetrachloride21. 1,1,1-trichloroethane22. 2-butanone23. benzene24. 1,2-dichloroethane25. methyl cyclohexane26. trichloroethene27. 1,4-difluorobenzene28. 1,2-dichloropropane

29. bromodichloromethane30. cis-1,3-dichloropropene31. toluene32. tetrachloroethene33. 4-methyl-2-pentanone34. trans-1,3-dichloropropene35. 1,1,2-trichloroethane36. dibromochloromethane37. 1,2-dibromoethane38. 2-hexanone39. chlorobenzene-d540. chlorobenzene41. ethylbenzene42. m-xylene

43. p-xylene44. o-xylene45. styrene46. bromoform47. isopropylbenzene48. 1,1,2,2-tetrachloroethane49. 1,3-dichlorobenzene50. 1,4-dichlorobenzene-d451. 1,4-dichlorobenzene52. 1,2-dichlorobenzene53. 1,2-dibromo-3-chloropropane54. 1,2,4-trichlorobenzene55. 1,2,3-trichlorobenzene

GC_EV00681

Rtx®-VMS 20m, 0.18mm ID, 1.0µm (cat.# 49915)Sample: Components at 10ppb (50ppb ketones) in 5mL RO water

OLC 03.2 VOA MegaMix™, cat.# 30492502.2 Calibration Mix #1 (gases), cat.# 30042VOA Calibration Mix #1 (ketones), cat.# 30006L/C VOA Internal Standard Mix, cat.# 30091

Concentrator: Tekmar 3100 Purge and TrapTrap: Vocarb 3000Purge: 11 min. @ 40mL/min. (ambient temperature)Dry purge: 1 min. @ 40mL/min. (MCS bypassed)Desorb preheat: 245°CDesorb: 250°C for 1 min. @ 40mL/min.

Bake: 260°C for 8 min. Interface: 30:1 split using 1/16" Silcosteel® tee (cat.# 20513)

& 0.3m of 0.25mm ID fused silica tubing for split line Carrier gas: 24psi helium, constant pressure Flow rate: 1.3 mL/min. @ 45°CDead time: 0.77 min. @ 45°COven temp.: 45°C (hold 4 min.) to 225°C @ 32°C/min. (hold 2 min.)Det: Agilent 5971A GC/MS w/ K&M electron multiplierTransfer line temp.: 280°CScan range: 35–260 amuTune: PFTBA/BFBIonization: EI

• Fast analysis time

528-544_GCAPP.qxd 11/3/2004 5:02 PM Page 537

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538

Volatile OrganicsUS EPA Method 8260 (80ppb Standard)Rtx®-VMS

GC_EV00685

Column: Rtx®-VMS, 30m, 0.25mm ID, 1.4µm (cat.# 19915)Sample: Calibration, internal standard, surrogate standard

mixes (cat.# 30475B, 30465, 30006, 30240, 30074)PPuurrggee aanndd ttrraapp ccoonnddiittiioonnss:: Trap: #10 (Tenax®/silica gel/carbon molecular sieve)Purge Time: 11 min.Purge Flow Rate: 38mL/min.Desorb Flow Rate: 32mL/min.Desorb Time: 1.0 min.Bake Time: 10 min.Sample Size: 10mLWater Management: 110°C purge, 0°C desorb, 240°C bakeSplit Ratio: 1:25TTeemmppeerraattuurreess::Sample: 40°CTrap: 20°C purge, 190°C desorb, 210°C bake6-Port Valve: 110°C

Transfer Line: 110°CSparge Mount: 45°CDesorb Preheat: 150°CValve Manifold: 50°COther Conditions: pre-purge, pre-heat, dry purge OFFCChhrroommaattooggrraapphhyy::Inj. temp.: 250°CCarrier gas: helium, constant flow Flow rate: 1.3 mL/min. Dead Time: 1.47 min. @ 35°COven temp.: 35°C (hold 7 min.) to 90°C @ 4°C/min.(no hold)

to 220°C @ 45°C/min. (hold 1 min.). Det.: Agilent 5971A GC/MSTransfer line temp.: 280°CScan range: 35-260 amuTune: PFTBA/BFB

*carbon dioxide 1. dichlorodifluoromethane 2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane 7. diethylether8. 1,1-dichloroethene9. carbon disulfide

10. Freon® 11311. iodomethane12. allyl chloride13. methylene chloride14. acetone15. trans-1,2-dichloroethene16. methyl-d3-tert-butyl-ether17. methyl acetate18. methyl-tert-butyl-ether19. tert-butyl alcohol20. acetonitrile21. diisopropyl ether22. chloroprene23. 1,1-dichloroethane24. acrylonitrile25. ethyl-tert-butyl ether

26. cis-1,2-dichloroethene27. 2,2-dichloropropane28. bromochloromethane29. chloroform30. carbon tetrachloride31. tetrahydrofuran32. methyl acrylate33. 1,1,1-trichloroethane34. dibromofluoromethane35. 1,1-dichloropropene36. 2-butanone37. benzene38. propionitrile39. methacrylonitrile40. 1,2-dichloroethane-d441. pentafluorobenzene42. tert-amyl-methyl ether43. 1,2-dichloroethane44. isobutyl alcohol45. trichloroethene46. 1,4-difluorobenzene47. dibromomethane48. 1,2-dichloropropane49. bromodichloromethane50. methyl methacrylate51. cis-1,3-dichloropropene

52. 2-chloroethyl vinyl ether53. toluene-d854. toluene55. 2-nitropropane56. tetrachloroethene57. 2-bromo-1-chloropropane58. 4-methyl-2-pentanone59. trans-1,3-dichloropropene60. 1,1,2-trichloroethane61. ethyl methacrylate62. dibromochloromethane63. 1,3-dichloropropane64. 1,2-dibromoethane65. 2-hexanone66. chlorobenzene-d567. chlorobenzene68. ethylbenzene69. 1,1,1,2-tetrachloroethane70. m-xylene71. p-xylene72. o-xylene73. bromoform74. stryrene75. isopropylbenzene76. 4-bromo-1-fluorobenzene (SS)77. bromobenzene

78. cis-1,4-dichloro-2-butene79. 1,4-dichlorobutane80. n-propylbenzene81. 1,1,2,2-tetrachloroethane82. 2-chlorotoluene83. 1,2,3-trichloropropane84. 1,3,5-trimethylbenzene85. trans-1,4-dichloro-2-butene86. 4-chlorotoluene87. tert-butylbenzene88. pentachloroethane89. 1,2,4-trimethylbenzene90. sec-butylbenzene91. p-isopropyltoluene92. 1,3-dichlorobenzene93. 1,4-dichlorobenzene-d494. 1,4-dichlorobenzene95. n-butylbenzene96. 1,2-dichlorobenzene97. 1,2-dibromo-3-chloropropane98. nitrobenzene99. hexachlorobutadiene

100. 1,2,4-trichlorobenzene101. naphthalene102. 1,2,3-trichlorobenzene

GC_EV00686

Acknowledgments: Purge & trap courtesy of O.I. Analytical.

528-544_GCAPP.qxd 11/3/2004 5:02 PM Page 538

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NTA

L539

Volatile OrganicsUS EPA Method 8240 (8260 Short List)Rtx®-VMS

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. ethanol8. 1,1-dichloroethene9. carbon disulfide

10. iodomethane11. allyl chloride12. methylene chloride13. acetone14. trans-1,2-dichloroethene15. 1,1-dichloroethane

16. acrylonitrile17. allyl alcohol18. vinyl acetate19. bromochloromethane20. chloroform21. carbon tetrachloride22. propargyl alcohol23. 1,1,1-trichloroethane24. 2-butanone25. benzene26. propionitrile27. methacrylonitrile28. 1,2-dichloroethane-d429. isobutyl alcohol30. 1,2-dichloroethane

31. trichloroethene32. 1,4-difluorobenzene33. dibromomethane34. 1,2-dichloropropane35. bromodichloromethane36. methyl methacrylate37. 1,4-dioxane38. 2-chloroethanol 39. 2-chloroethyl vinyl ether40. cis-1,3-dichloropropene41. toluene-d842. toluene43. pyridine44. 4-methyl-2-pentanone45. tetrachloroethene

46. trans-1,3-dichloropropene47. ethyl methacrylate48. 1,1,2-trichloroethane49. dibromochloromethane50. 1,2-dibromoethane51. 2-hexanone52. 2-picoline53. chlorobenzene-D554. ethylbenzene55. chlorobenzene56. 1,1,1,2-tetrachloroethane57. m-xylene58. p-xylene59. o-xylene60. styrene

61. bromoform62. 4-bromo-1-fluorobenzene 63. cis-1,4-dichloro-2-butene64. 1,1,2,2-tetrachloroethane65. 1,2,3-trichloropropane66. trans-1,4-dichloro-2-butene67. pentachloroethane68. 1,3-dichlorobenzene69. 1,4-dichlorobenzene70. benzyl chloride71. malononitrile 72. 1,2-dichlorobenzene73. 1,2-dibromo-3-chloropropane

TToopp cchhrroommaattooggrraamm::Oven temp.: 40°C (hold 4 min.) to 90°C @ 16°C/min. (no hold)

to 210°C @ 32°C/min. (hold 5 min.) Adjust dichlorodifluoromethane to a retention time of 2.27 min. @ 40°C.

MS Scan Range: 35-300amucompound concentrations, by mix: (in 5mL of RO water)Compounds at 100ppb (cat.# 30213, 30004, 30006, 30011, 30042)Alcohols at 1ppm (cat.# 30214) except 2-chloroethanol at 10ppmvinyl acetate at 500ppb (cat.#30216)8240 Nitrile Mix at 200ppb (cat.# 30215)8240 Mix 1A at 300ppb (cat.# 30217)8240 Mix 2A at 500ppb (cat.# 30218)

BBoottttoomm cchhrroommaattooggrraamm::Oven temp.: 45°C (hold 4 min.) to 110°C @ 19°C/min. (hold 5

min.) to 220°C @ 32°C/min. (hold 5 min.) Adjust dichlorodifluoromethane to 2.23 min. @ 45°C.

MS Scan Range: 29-260amu, for 2-chloroethanol responsecompound concentrations, by mix: (in 5mL of RO water)Compounds at 100ppb (cat.# 30213, 30004, 30006, 30011, 30042)Alcohols at 1ppm (cat.# 30214) (see MS scan)vinyl acetate at 100ppb (cat.# 30216)8240 Nitrile Mix at 400ppb (cat.# 30215)8240 Mix 1A at 300ppb (cat.# 30217)8240 Mix 2A at 500ppb (cat.# 30218)

IInnssttrruummeennttaattiioonn && CCoonnddiittiioonnss CCoommmmoonn ttoo BBOOTTHH RRUUNNSS…Carrier gas: 1.3mL/min. @ constant flowConcentrator: Tekmar LSC-3000 Purge and TrapTrap: Vocarb 3000 (type K)Purge: 11 min. @ 40mL/min. (ambient temp.)Dry purge: 1 min. @ 40mL/min. (MCS bypass)Desorb preheat: 245°CDesorb: 250°C for 2 min., flow 26mL/min.Bake: 260°C for 8 min.Interface to GC: transfer line 0.32mm ID Siltek™ fused silica

1:20 split at injection port w/ 1mm ID liner. GC: HP 6890Detector: HP 5973 MSD

Rtx®-VMS30m, 0.25mm ID, 1.40µm (cat.# 19915)

min. 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00

1

23 4

56

8-9

710

1112

1314

1516

17

18

1920

21,22

2324

25 26-27

28,29

30

31,32

33

34 35

36,37

38

39 40

41 42

43

44-46

47

48

4950

51

52

53-56

57,58

59,60

61

62,6364 65,66

6768

69 70

71

72

73

1

2

3

4

6

7

8,9

10

11

12

13

1415

16

17,18

192021

22,23

24

25-27

28,29

30

31,32

33

34

35

36

37

38

39

40

41 42

43

4445,46

47

48

49

50 51

52

53-55

56

57,58

60,61

59

6263

6566

64

67

6869

70

72

71

73

min. 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00

5

methanol

slow

er ru

n re

solv

es th

ese

GC_EV00426

• Standard wastewater analysis(35–300amu)

• Scan range 29–260amu, fordetecting 2-chloroethanol

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Volatile OrganicsUS EPA Method 8260BRtx®-VMS

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. ethanol (2500ppb)8. 1,1-dichloroethene9. carbon disulfide (40ppb)

10. allyl chloride11. methylene chloride12. acetone13. trans-1,2-dichloroethene14. tert-butyl alcohol (100ppb)15. methyl tert-butyl ether16. diisopropyl ether17. 1,1-dichloroethane18. acrylonitrile19. vinyl acetate*20. allyl alcohol (250ppb)21. ethyl-tert-butyl ether*22. cis-1,2-dichloroethene23. 2,2-dichloropropane24. bromochloromethane25. chloroform

26. ethyl acetate27. methyl acrylate28. propargyl alcohol (500ppb)29. dibromofluoromethane (SMC)30. tetrahydrofuran31. carbon tetrachloride32. 2-butanone33. 1,1,1-trichloroethane34. 1,1-dichloropropene35. pentafluorobenzene (IS)36. tert-amyl methyl ether37. benzene38. isobutyl alcohol (500ppb)39. 1,2-dichloroethane40. isopropyl acetate41. 1,4-difluorobenzene (SMC)42. trichloroethene43. dibromomethane44. bromodichloromethane45. 1,2-dichloropropane46. methyl methacrylate47. n-propyl acetate48. 2-chloroethanol (2500ppb)49. cis-1,3-dichloropropene50. toluene-d8 (SMC)

51. toluene52. 4-methyl-2-pentanone53. pyridine (250ppb)54. trans-1,3-dichloropropene55. ethyl methacrylate56. tetrachloroethene57. 1,1,2-trichloroethane58. dibromochloromethane59. 1,3-dichloropropane60. n-butyl acetate61. 1,2-dibromoethane62. 2-hexanone63. 2-picoline (250ppb)64. ethylbenzene65. chlorobenzene-D5 (IS)66. chlorobenzene67. 1,1,1,2-tetrachloroethane68. m-xylene69. p-xylene70. o-xylene71. styrene72. bromoform73. isopropylbenzene74. 4-bromo-1-fluorobenzene (SMC)75. n-propylbenzene

76. 1,1,2,2-tetrachloroethane77. bromobenzene78. 1,3,5-trimethylbenzene79. 2-chlorotoluene80. 1,2,3-trichloropropane81. 4-chlorotoluene82. tert-butylbenzene83. 1,2,4-trimethylbenzene84. pentachloroethane85. sec-butylbenzene86. p-isopropyltoluene87. 1,3-dichlorobenzene88. 1,4-dichlorobenzene-d4 (IS)89. 1,4-dichlorobenzene90. n-butylbenzene91. 1,2-dichlorobenzene92. 1,2-dibromo-3-chloropropane93. nitrobenzene (250ppb)94. hexachlorobutadiene95. 1,2,4-trichlorobenzene96. naphthalene97. 1,2,3-trichlorobenzene

min. 6 8 10 12 14 16

60m, 0.25 mm ID, 1.40µm Rtx®-VMS (cat.# 19916)Conc.: 10ppb in 5mL of RO water (unless noted);

ketones 2.5XConcentrator: Tekmar LSC-3100 Purge and TrapTrap: Vocarb 3000 (type K)Purge: 11 min. @ 40mL/min. (ambient temperature)Dry purge: 1 min. @ 40mL/min.Desorb preheat: 245°C Desorb: 250°C for 2 min., flow 26mL/min.Bake: 260°C for 8 min.Interface: 0.53mm ID Silcosteel® tubing transfer line

1:20 split at injection port. 1mm ID liner.Oven temp.: 60°C (hold 2 min.) to 180°C @ 12°C/min. (hold 0 min.) to

225°C @ 45°C/min. (hold 6 min.)Carrier gas: helium @ ~1.3mL/min. constant flow

Adjust dichlorodifluoromethane to a retention time of 4.03 min. @ 60°C.

Detector: Agilent 5973 MSDScan range: 35-300amu

1

2

3

4 5

6

7,8

9

10

11,12

13-15

16

17,1819-21

2223

24,25

28

29-3334

35

36-38

39,40

41,42

43

44-46

47

48

49

50

51

52,53

54

55-56

57

58

59

60

61

62

63

64-67

68,69

70

71

72

73

74

75

76,77

78

79,80

81

8283,84

8586

87

88,89

90

91

92

93,94

95

96

97

27

26

GC_EV00427

*Peaks 19 & 21 share an ion (43).

60°C starting temperature• fast analyses• fast cycles

Reduce starting temperature tobest focus the gases

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1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. ethanol (2500ppb)8. 1,1-dichloroethene9. carbon disulfide (40ppb)

10. allyl chloride11. methylene chloride12. acetone13. trans-1,2-dichloroethene14. methyl tert-butyl ether15. tert-butyl alcohol (100ppb)16. diisopropyl ether17. 1,1-dichloroethane18. acrylonitrile19. vinyl acetate20. allyl alcohol (250ppb)21. ethyl-tert-buyl ether22. cis-1,2-dichloroethene23. 2,2-dichloropropane24. bromochloromethane25. chloroform

26. ethyl acetate27. carbon tetrachloride28. methyl acrylate29. propargyl alcohol (500ppb)30. dibromofluoromethane (SMC)31. tetrahydrofuran32. 1,1,1-trichloroethane33. 2-butanone34. 1,1-dichloropropene35. benzene36. pentafluorobenzene (IS)37. tert-amyl-methyl ether38. 1,2-dichloroethane39. isobutyl alcohol (500ppb)40. isopropyl acetate41. trichloroethene42. 1,4-difluorobenzene (SMC)43. dibromomethane44. 1,2-dichloropropane45. bromodichloromethane46. methyl methacrylate47. n-propyl acetate48. 2-chloroethanol (2500ppb)49. cis-1,3-dichloropropene50. toluene-d8 (SMC)

51. toluene52. pyridine

(250ppb)53. tetrachloroethene54. 4-methyl-2-pentanone55. trans-1,3-dichloropropene56. 1,1,2-trichloroethane57. ethyl methacrylate58. dibromochloromethane59. 1,3-dichloropropane60. 1,2-dibromoethane61. n-butyl acetate62. 2-hexanone63. 2-picoline (250ppb)64. chlorobenzene-D5 (IS)65. chlorobenzene66. ethylbenzene67. 1,1,1,2-tetrachloroethane68. m-xylene69. p-xylene70. o-xylene71. stryrene72. bromoform73. isopropylbenzene74. 4-bromo-1-fluorobenzene (SMC)

75. bromobenzene76. n-propylbenzene77. 1,1,2,2-tetrachloroethane78. 2-chlorotoluene79. 1,3,5-trimethylbenzene80. 1,2,3-trichloropropane81. 4-chlorotoluene82. tert-butylbenzene83. pentachloroethane84. 1,2,4-trimethylbenzene85. sec-butylbenzene86. p-isopropyltoluene87. 1,3-dichlorobenzene88. 1,4-dichlorobenzene-d4 (IS)89. 1,4-dichlorobenzene90. n-butylbenzene91. 1,2-dichlorobenzene92. 1,2-dibromo-3-chloropropane93. nitrobenzene (250ppb)94. hexachlorobutadiene95. 1,2,4-trichlorobenzene96. naphthalene97. 1,2,3-trichlorobenzene

20m, 0.18 mm ID, 1.00µm Rtx®-VMS (cat.# 49914)Conc.: 10ppb in 5mL of RO water

unless otherwise noted; ketones at 2.5XConcentrator: Tekmar LSC-3100 Purge and TrapTrap: Vocarb 3000 (type K)Purge: 11 min. @ 40mL/min. (ambient temperature)Dry purge: 1 min. @ 40mL/min.Desorb preheat: 245°C Desorb: 250°C for 2 min., flow 40mL/min.Bake: 260°C for 8 min.Interface: 0.53mm ID Silcosteel® tubing transfer line

1:40 split at injection port. 1mm ID liner.Oven temp.: 50°C (hold 4 min.) to 100°C @ 18°C/min. (hold 0 min.)

to 230°C @ 40°C/min. (hold 3 min.)Carrier gas: helium @ ~1.0mL/min. constant flow

Adjust dichlorodifluoromethane to a retention time of 1.03 min. @ 50°C.Detector: Agilent 5973 MSDScan range: 35-300amu

min. 2 4 6 8 10

2

13

45

6

7

8

9 10

1113

1415

16

17

18

19-212223

24

25

26-2829-32

33,34

35

36 37

38

39

40

41

42

43

4445

46 4748

49

50

51

52

53

54,55

56

57

58

59

6061

62,63

64,65

66,67

68,69

70

71,72

73

74

75

76

77

78

79,80

81

82,83

8485

86

87

88,8990

91

92

93

94

95

96

97

GC_EV00428

12

• First choice for use with dual purge & traps1

• EPA recommended surrogate used.

1A.L. Hilling and G. Smith, Environmental Testing & Analysis, 10(3), 15-19, 2001.

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GC_EV00641

GC_EV00640

Column: Rtx®-VMS 40m, 0.18mm ID, 1.0µm (cat.# 49915)

Conc.: 5ppb each in 25mL of RO waterConcentrator: Tekmar LSC-3100 Purge and TrapTrap: Vocarb 3000Purge: 11 min. @ 40mL/min.Dry purge: 1 min. @ 40mL/min. (MCS by-passed

with Silcosteel® tubing, cat.# 21035)Desorb preheat: 245°CDesorb: 2 min. @ 250°C Desorb flow rate: 45mL/min. heliumBake: 8 min. @ 260°C Interface: plumbed through injection portTransfer line: Silcosteel® transfer line (cat.# 20591)Mount Temp: 40°C

Split ratio: 1:40 split Inlet liner: Agilent Split 1mm Liner (cat.# 20972) Inj. temp.: 250°CCarrier gas: 1.1mL/min. helium, constant flowLinear velocity: 32cm/sec. @ 40°C Oven temp.: 35°C (hold 2 min.) to 60°C @

4°C/min. (hold 0 min.) to 225°C @ 40°C/min. (hold 5 min.)

Detector: 5973 GC/MS w/ turbomolecular pumpSource Temp.: 280°CScan range: 35–260amuIonization: EI

1. carbon dioxide2. dichlorodifluoromethane3. Freon® 1144. chloromethane5. vinyl chloride6. bromomethane7. chloroethane8. trichlorofluoromethane9. ethanol

10. diethyl ether11. 1,1-dichloroethene12. iodomethane13. carbon disulfide14. Freon® 11315. acrolein16. allyl chloride17. methylene chloride18. acetone19. trans-1,2-dichloroethene20. methyl acetate21. methyl tert-butyl ether22. tert-butyl alcohol23. acetonitrile

24. diisopropyl ether25. chloroprene26. 1,1-dichloroethane27. acrylonitrile28. vinyl acetate**29. ethyl-tert-butyl ether**30. cis-1,2-dichloroethene31. 2,2-dichloropropane32. bromochloromethane33. chloroform34. carbon tetrachloride35. ethyl acetate36. methyl acrylate37. dibromofluoromethane38. tetrahydrofuran39. 1,1,1-trichloroethane40. 2-butanone41. 1,1-dichloropropene42. benzene43. propionitrile44. methacrylonitrile45. pentafluorobenzene46. tert-amyl-methyl ether

47. 1,2-dichloroethane48. isobutyl alcohol 49. isopropyl acetate50. trichloroethene51. 1,4-difluorobenzene52. dibromomethane53. 1,2-dichloropropane54. bromodichloromethane55. methyl methacrylate56. n-propyl acetate57. 2-chloroethyl vinyl ether58. cis-1,3-dichloropropene59. toluene-d860. toluene61. 2-nitropropane62. tetrachloroethene63. 4-methyl-2-pentanone64. trans-1,3-dichloropropene65. 2-bromo-1-chloropropane66. 1,1,2-trichloroethane67. ethyl methacrylate68. dibromochloromethane69. 1,3-dichloropropane

70. 1,2-dibromoethane71. n-butyl acetate72. 2-hexanone73. chlorobenzene-d574. chlorobenzene75. ethylbenzene76. 1,1,1,2-tetrachloroethane77. m-xylene78. p-xylene79. o-xylene80. styrene81. bromoform82. isopropylbenzene83. n-amyl acetate84. 4-bromo-1-fluorobenzene (ss)85. cis-1,4-dichloro-2-butene86. n-propylbenzene87. bromobenzene88. 1,4-dichlorobutane89. 1,1,2,2-tetrachloroethane90. 2-chlorotoluene91. 1,3,5-trimethylbenzene92. 1,2,3-trichloropropane

93. trans-1,4-dichloro-2-butene94. 4-chlorotoluene95. tert-butylbenzene96. pentachloroethane97. 1,2,4-trimethylbenzene98. sec-butylbenzene99. p-isopropyltoluene

100. 1,3-dichlorobenzene101. 1,4-dichlorobenzene-d4102. 1,4-dichlorobenzene103. n-butylbenzene104. 1,2-dichlorobenzene105. 1,2-dibromo-3-chloropropane106. nitrobenzene107. hexachlorobutadiene108. 1,2,3-trichlorobenzene109. naphthalene110. 1,2,4-trichlorobenzene

RReeffeerreennccee SSttaannddaarrdd ccaatt..##8260B Calibration Mix #1* 30475502.2 Calibration Mix #1 (gases) 30042VOA Calibration Mix #1 (ketones) 300068260 Internal Standard Mix 300748260 Surrogate Mix 300738260 Acetate Mix 30489California Oxygenates Mix 30465Acrolein Mix 30478Ethanol Mix 30466Freon® 114 30476

*2-Chloroethanol in 8260B mix requires lower scan range for identification; 1,4-dioxane requires fortification or identification using single point high calibration standard.**Peaks 28 & 29 share ion (43).

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Volatile OrganicsUS EPA Method 524.2 Revision IVRtx®-VMS

PPuurrggee aanndd TTrraapp CCoonnddiittiioonnss:: Concentrator: Tekmar LSC-3100 purge and trapTrap: Vocarb 3000 (type K) Purge: 11 min. @ 40 mL/min. @ ambient temperature. Dry purge: 1 min. @ 40mL/min. (MCS bypassed using Silcosteel® tubing)Desorb preheat: 245°C Desorb: 250°C for 2 min., flow 10mL/min.Bake: 260°C for 8 min.Interface: Silcosteel® transfer line

1:30 split at injection port. 1mm ID split injection sleeve (cat.# 20972).

Column: Rtx®-VMS, 30m, 0.25mm ID, 1.4µm (cat.# 19915)Sample: 502.2 Calibration Mix #1 (cat.# 30042)

Drinking Water VOA MegaMix™, 524.2 Rev 4 (cat.# 30601)524 Internal Standard/Surrogate Mix (cat.# 30201)Ketone Mix, EPA Method 524.2 Rev 4.1 (cat.# 30602)Compounds at 20 ppb each in 5mL RO water(ketones at 50ppb; internal standards at 40ppb)

Inj. temp.: 250°CCarrier gas: helium, constant flow Flow rate: 1.1mL/min. Dead time: 1.48 min. @ 40°COven temp.: 40°C (hold 2 min.) to 85°C @ 14°C/min. (hold 2 min.) to

220°C @ 30°C/min. (hold 4 min.). Det: Agilent 5971A GC/MSTransfer line temp.: 280°CScan range: 35-300 amuTune PFTBA/BFBIonization: EI

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. diethyl ether8. 1,1-dichloroethene9. carbon disulfide

10. iodomethane11. allyl chloride12. methylene chloride13. acetone14. trans-1,2-dichloroethene15. methyl tert-butyl ether16. 1,1-dichloroethane17. acrylonitrile18. cis-1,2-dichloroethene

19. 2,2-dichloropropane20. bromochloromethane21. chloroform22. methyl acrylate23. carbon tetrachloride24. tetrahydrofuran25. 1,1,1-trichloroethane26. 2-butanone27. 1,1-dichloropropene28. 1-chlorobutane29. propionitrile30. methacrylonitrile31. benzene32. 1,2-dichloroethane33. fluorobenzene34. trichloroethene35. dibromomethane36. 1,2-dichloropropane

37. bromodichloromethane38. methyl methacrylate39. cis-1,3-dichloropropene40. toluene41. chloroacetonitrile42. 2-nitropropane43. 1,1-dichloropropanone44. 4-methyl-2-pentanone45. tetrachloroethene46. trans-1,3-dichloropropene47. 1,1,2-trichloroethane48. ethyl methacrylate49. dibromochloromethane50. 1,3-dichloropropane51. 1,2-dibromoethane52. 2-hexanone53. m-xylene54. p-xylene

55. chlorobenzene56. ethylbenzene57. 1,1,1,2-tetrachloroethane58. o-xylene59. styrrene60. bromoform61. isopropylbenzene62. 4-bromofluorobenzene 63. bromobenzene64. n-propylbenzene65. 1,1,2,2-tetrachloroethane66. 2-chlorotoluene67. 1,2,3-trichloropropane68. 1,3,5-trimethylbenzene69. trans-1,4-dichloro-2-butene70. 4-chlorotoluene71. tert-butylbenzene72. pentachloroethane

73. 1,2,4-trimethylbenzene74. sec-butylbenzene75. p-isopropyltoluene76. 1,3-dichlorobenzene77. 1,4-dichlorobenzene78. n-butylbenzene79. hexachloroethane80. 1,2-dichlorobenzene-d481. 1,2-dichlorobenzene82. 1,2-dibromo-3-chloropropane83. nitrobenzene84. hexachlorobutadiene85. 1,2,4-trichlorobenzene86. naphthalene87. 1,2,3-trichlorobenzene

GC_EV00718

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Volatile OrganicsUS EPA Method 524.2Rtx®-502.2

12

34 5

67

8

9 10

13

16 17

2024

25

2627

2829

30

31

32

33,34

35,36

39

40

42

43

44

454647

4849

5051

52 53

54

55

59 60

11

12 14 15 21

23

22

37 38

41 58

min. 5 10 15 20 25 30 35

18,19

GC_EV00010

40m, 0.18mm ID, 1.0µm Rtx®-502.2 (cat.# 40915)Conc.: Reference Standard 524 Volatiles Kit (cat. # 30052)

200ppb VOA standards in 5mL RO water.Purge & trap: Tekmar LCS 2000Trap: Vocarb 4000Purge: 11 min.Desorb preheat: 250°CDesorb time: 2 min.

Desorb flow rate: 30mL/min.Oven temp.: 35°C (hold 4 min.) to 150°C @ 6°C/min. to

220°C @ 8°C/min. (hold 8 min.).Inj. / det. temp.: 200°C / 250°CDetector: MSLinear velocity: 21cm/sec. set @ 35°CSplit ratio: 30:1

56

57

1. dichlorodifluoromethane2. chloromethane3. vinyl chloride4. bromomethane5. chloroethane6. trichlorofluoromethane7. 1,1-dichloroethene8. methylene chloride9. trans-1,2-dichloroethene

10. 1,1-dichloroethane11. 2,2-dichloropropane12. cis-1,2-dichloroethene13. bromochloromethane

14. chloroform15. 1,1,1-trichloroethane16. 1,1-dichloropropene17. carbon tetrachloride18. benzene19. 1,2-dichloroethane20. trichloroethene21. 1,2-dichloropropane22. bromodichloromethane23. dibromomethane24. cis-1,3-dichloropropene25. toluene26. trans-1,3-dichloropropene

27. 1,1,2-trichloroethane28. 1,3-dichloropropane29. tetrachloroethene30. dibromochloromethane31. ethylene dibromide32. chlorobenzene33. ethylbenzene34. 1,1,1,2-tetrachloroethane35. m-xylene36. p-xylene37. o-xylene38. styrene39. isopropylbenzene

40. bromoform41. 1,1,2,2-tetrachloroethane42. 1,2,3-trichloropropane43. propylbenzene44. bromobenzene45. 1,3,5-trimethylbenzene46. 2-chlorotoluene47. 4-chlorotoluene48. tert-butylbenzene49. 1,2,4-trimethylbenzene50. sec-butylbenzene51. p-isopropyltoluene52. 1,3-dichlorobenzene

53. 1,4-dichlorobenzene54. n-butylbenzene55. 1,2-dichlorobenzene56. 1,2-dibromo-3-chloro-

propane57. 1,2,4-trichlorobenzene58. hexachlorobutadiene59. naphthalene60. 1,2,3-trichlorobenzene

a plus 1 storyThe Web Team members have worked very hard to develop a new andimproved website that makes it easier for our customers to find informa-tion about our products. Visitors have complimented our new website onits ease of use, and we have more visitors to the site each day.

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www.restek.com

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PhenolsUS EPA Method 604 MXT®-5

1

2 3

4

5

6

7

8

910

11

min. 4 8 12 16

Reference Standard604 Phenols Mix (cat. # 31029):

1. phenol2. 2-chlorophenol3. 2-nitrophenol4. 2,4-dimethylphenol5. 2,4-dichlorophenol6. 4-chloro-3-methylphenol7. 2,4,6-trichlorophenol8. 2,4-dinitrophenol9. 4-nitrophenol

10. 2-methyl-4,6-dinitrophenol11. pentachlorophenol

GC_EV0002730m, 0.28mm ID, 0.25µm MXT®-5 (cat.# 70224)1.0µL splitless injection of phenols.Concentration 25ng/µL per component.

Oven temp.: 40°C to 250°C @ 10°C/min.Inj. / det. temp.: 280°C / 300°CCarrier gas: hydrogenLinear velocity: 50cm/sec. set @ 40°CFID sensitivity: 2.56 x 10-10 AFS

PhenolsUS EPA Method 604Rtx®-200

30m, 0.32mm ID, 0.25µm Rtx®-200 (cat.# 15024)1.0µL split injection of a 200ng standard.

Oven temp.: 50°C (hold 4 min.) to 250°C @ 6°C/min.Inj. & det. temp.: 250°CCarrier gas: heliumLinear velocity: 20cm/sec. FID sensitivity: 4 x 10-11 AFSSplit ratio: 40:1

11

109

8

6

54

2

1

min. 4 8 12 16 20 24 28

7

3

GC_EV00028


1. phenol2. 2-chlorophenol3. 2,4-dimethylphenol4. 2,4-dichlorophenol5. 2-nitrophenol6. 4-chloro-3-methylphenol7. 2,4,6-trichlorophenol8. 4-nitrophenol9. pentachlorophenol

10. 2,4-dinitrophenol11. 2-methyl-4,6-dinitrophenol


30m, 0.25mm ID, 0.25µm Rtx®-50 (cat.# 10523)1.0µL split injection of phenols.Concentration 3–5ng/µL per component.

Oven temp.: 50°C (hold 10 min.) to 250°C @ 15°C/min. (hold 15 min.)

Inj. & det. temp.: 280°CCarrier gas: heliumLinear velocity: 20cm/sec. (flow rate: 0.7cc/min.)FID sensitivity: 32 x 10-11 AFSSplit vent: 40cc/min.

min. 4 8 12 16 20 24 28

11

10

98

7

6

54

321

GC_EV00029


1. phenol2. 2-chlorophenol3. 2,4-dimethylphenol4. 2-nitrophenol5. 2,4-dichlorophenol6. 4-chloro-3-methylphenol7. 2,4,6-trichlorophenol8. 2,4-dinitrophenol9. 4-nitrophenol



30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)1.0µL split injection of phenols.Concentration 3-5ng/µL per component.

Oven temp.: 50°C (hold 4 min.) to 250°C @ 8°C/min.

Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. set @ 110°CFID sensitivity: 8 x 10-11 AFSSplit ratio: 40:1

min. 4 8 12 16 20 24

1

2

35

6

78

9

10

11

4

GC_EV00030




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PhenolsXTI®-5

min. 5 10 15 20 25 30 35

GC_EV00031

IS6

IS5

IS4

15

14

1312

7

5

864

3

IS1

21

IS2

9

1110

IS3

Reference StandardSV Calibration Mix #2 (cat.# 31008)

1. phenol2. 2-chlorophenol3. 2-methylphenol4. 4-methylphenol5. 2-nitrophenol6. 2,4-dimethylphenol7. benzoic acid8. 2,4-dichlorophenol9. 4-chloro-3-methylphenol

10. 2,4,6-trichlorophenol11. 2,4,5-trichlorophenol12. 2,4-dinitrophenol13. 4-nitrophenol14. 2-methyl-4,6-dinitrophenol15. pentachlorophenol

SV Internal Std. Mix (cat.# 31006):IS1. dichlorobenzene-d4IS2. naphthalene-d8IS3. acenaphthene-d10IS4. phenanthrene-d10IS5. chrysene-d12IS6. perylene-d12

30m, 0.25mm ID, 0.25µm XTI®-5 (cat.# 12223)1.0µL splitless injection. 40ng of phenols and IS mix.

Oven temp.: 40°C to 350°C @ 15°C/min. (hold 15 min.)Inj. temp.: 350°CDetector: MSScan rate: 1.5 scan/sec.Scan range: 35-400AMU

Phenols & BenzidinesUS EPA Methods 604 & 605 Rtx®-5

30m, 0.32mm ID, 1.0µm Rtx®-5 (cat.# 10254)1.5µL split injection of benzidines and phenols.Concentration 1–3ng per component on-column.

Oven temp.: 110°C to 290°C @ 8°C/min.Inj. & det. temp.: 310°CCarrier gas: hydrogenLinear velocity: 40cm/sec. FID sensitivity: 2 x 10-11 AFSSplit ratio: 100:1

min. 4 8 12 16 20 24 28 32

GC_EV00032

13

12

1110

9

8

7

64

32

1Reference Standard604 Phenols Mix (cat. # 31029)605 Benzidines Mix (cat.# 31030):


10. 2-methyl-4,6-dinitrophenol11. pentachlorophenol12. benzidine13. 3,3'-dichlorobenzidine

5

Phenols & BenzidinesUS EPA Methods 604 & 605 Rtx®-5

30m, 0.53mm ID, 1.5µm Rtx®-5 (cat.# 10270)2.5µL direct injection of benzidines and phenols.Concentration approximately 25ng per component.


Inj. & det. temp.: 300°CCarrier gas: hydrogenLinear velocity: 80cm/sec. (flow rate: 10cc/min.) FID sensitivity: 8 x 10-11 AFS

3 5

4

6 7

8

9 10

11

12 13

GC_EV00033

min. 4 8 12 16 20 24 28 32

21

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Semivolatiles

Phthalate EstersUS EPA Method 606 Rtx®-5

min. 4 8 12 16 20 24 28 32

GC_EV00034

1

6

5

4

3

2

30m, 0.53mm ID, 1.5µm Rtx®-5 (cat.# 10270)2.5µL direct injection of phthalate esters.Concentration approximately 25ng per component.



Reference Standard606 Phthalate Esters Mix (cat.# 31031):

1. dimethyl phthalate2. diethyl phthalate3. di-n-butyl phthalate4. butyl benzyl phthalate5. bis(2-ethylhexyl)phthalate6. di-n-octyl phthalate

Phthalate EstersUS EPA Method 606 MXT®-5

30m, 0.53mm ID, 1.0µm MXT®-5 (cat.# 70255)0.5µL split injection of phthalate esters.Concentration 50ng per component.


Inj. & det. temp.: 300°CCarrier gas: hydrogenLinear velocity: 50cm/sec.FID sensitivity: 8 x 10-11 AFSSplit ratio: 50:1

Reference Standard606 Phthalate Esters Mix (cat.# 31031):

1. dimethyl phthalate2. diethyl phthalate3. di-n-butyl phthalate4. butyl benzyl phthalate5. bis(2-ethylhexyl)phthalate6. di-n-octyl phthalate

2

3

1

4

5

6

GC_EV00035

min. 4 8 12 16 20 24 28 32 36

Phthalate EstersUS EPA Method 8060Rtx®-50

30m, 0.53mm ID, 0.50µm Rtx®-50 (cat.# 10540)1.5µL direct injection of phthalate esters using a Uniliner® directinjection liner.Concentration approximately 60µg/mL per component.

Oven temp.: 100°C to 275°C @ 15°C/min. (hold 10 min.)

Inj. & det. temp.: 275°CCarrier gas: heliumLinear velocity: 20cm/sec. FID sensitivity: 64 x 10-11 AFS

1. dimethyl phthalate2. diethyl phthalate3. diisobutyl phthalate4. di-n-butyl phthalate5. bis(methoxyethyl) phthalate6. bis(4-methyl,2-pentyl) phthalate7. bis(ethoxyethyl) phthalate8. di-amyl phthalate 9. hexyl-2-ethylhexyl phthalate

10. di-n-hexyl phthalate11. butyl benzyl phthalate12. bis(2-n-butoxyethyl) phthalate13. dicyclohexyl phthalate14. bis(2-ethylhexyl)phthalate15. di-n-octyl phthalate16. di-nonyl phthalate

1 2 3

4

5

6

8

7910

12 13

14

1615

11

min. 4 8 12 16 20 24

GC_EV00037

a plus 1 story“Any time I have any type of chromatography question, I know that I can call Restek Technical Service forassistance. Every time I have spoken to them, they have been extremely helpful, and friendly!”

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NitrosaminesUS EPA Method 607Rtx®-5

15m, 0.53mm ID, 1.50µm Rtx®-5 (cat.# 10267)1.0µL direct injection of nitrosamines using a Uniliner®inlet liner.Concentration approximately 50ng/µL in methanol.


Inj. & det. temp.: 240°CCarrier gas: hydrogenLinear velocity: 80cm/sec. (flow rate: 10cc/min.)FID sensitivity: 128 x 10-11 AFS

3

2

1

min. 4 8 12 16 20

1. N-nitrosodimethylamine2. N-nitrosodiethylamine3. N-nitroso-di-n-propylamine4. diphenylamine (breakdown product of

N-nitrosodiphenylamine)

GC_EV00038

4

NitrosaminesUS EPA Method 607Stabilwax®

30m, 0.32mm ID, 1.0µm Stabilwax® (cat.# 10654)1.0µL split injection of nitrosamines.Concentration approximately 50ng/µL in methanol.


Inj. & det. temp.: 240°CCarrier gas: hydrogenLinear velocity: 80cm/sec. (flow rate: 10cc/min.)FID sensitivity: 128 x 10-11 AFSSplit ratio: 40:1

2

1

min. 4 8 12 16

4

1. N-nitrosodimethylamine2. N-nitrosodiethylamine3. N-nitroso-di-n-propylamine4. diphenylamine (breakdown product

of N-nitrosodiphenylamine)

3

GC_EV00039

NitrosaminesRtx®-200

30m, 0.53mm ID, 0.50µm Rtx®-200 (cat.# 15040)1.0µL split injection of nitrosamines.Concentration 10µg/mL.

Oven temp.: 100°C (hold 1 min.) to 200°C @ 5°C/min. Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.2cc/min.)FID sensitivity: 16 x 10-12 AFSSplit vent: 40cc/min.

min. 2 4 6 8GC_EV00040

87

6

54

32

1

1. N-nitrosodimethylamine2. N-nitrosomethylethylamine3. N-nitrosodiethylamine4. N-nitrosoazetidine5. N-nitrosopiperidine6. N-nitrosomorpholine7. N-nitrosopyrrolidine8. N-nitrosohexamethyleneimine

NitroaromaticsUS EPA Method 609Rtx®-5

15m, 0.53mm ID, 1.50µm Rtx®-5 (cat.# 10267)0.25µL split injection of nitroaromatics.On-column concentration 28ng/component.

Oven temp.: 100°C to 300°C @ 15°C/min. (hold 10 min.)Inj. / det. temp.: 250°C / 300°CCarrier gas: heliumLinear velocity: 23cm/sec. set @ 40°CFID sensitivity: range = 4, atten. = 3

2

1

3 4

min. 4 8 12 16

GC_EV00041

Reference Standard 609 Nitroaromatics/Isophorone Mix (cat.# 31033):

1. nitrobenzene2. isophorone3. 2,6-dinitrotoluene4. 2,4-dinitrotoluene

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Column: Rtx®-CLPesticides2 30m, 0.25mm ID, 0.20µm (cat.# 11323)Sample: 50µg/mL 610 PAH Mix (cat.# 31011)

in methylene chloride Inj.: 1.0µL splitless (hold 2 min.),

2mm splitless inlet liner w/ wool (cat.# 20829)Inj. temp.: 330°CCarrier gas: hydrogen, 4mL/min. constant flow Oven temp.: 40°C (hold 2 min.) to 250°C @ 25°C/min. to 265°C @ 5°C/min.

to 300°C (hold 4 min.) @25°C/min.Det.: FID @ 350°C

1. naphthalene2. acenaphthylene3. acenaphthene4. fluorene5. phenanthrene6. anthracene7. fluoranthene8. pyrene9. benzo(a)anthracene

10. chrysene11. benzo(b)fluoranthene12. benzo(k)fluoranthene13. benzo(a)pyrene14. indeno(1,2,3-cd)pyrene15. dibenzo(a,h)anthracene16. benzo(ghi)perylene

GC_EV00596

Polynuclear Aromatic HydrocarbonsUS EPA Method 610Rtx®-CLPesticides2

Column: Rtx®-5Sil MS 30m, 0.25mm ID, 0.25µm (cat.# 12723)Sample: 50µg/mL 610 PAH Mix (cat.# 31011)

in methylene chloride Inj.: 1.0µL splitless (hold 2 min.),

2mm splitless inlet liner w/ wool (cat.# 20829)Inj. temp.: 330°CCarrier gas: hydrogen, 4mL/min. constant flow Oven temp.: 40°C (hold 2 min.) to 250°C @ 25°C/min. to 265°C @ 5°C/min.

to 300°C (hold 4 min.) @ 25°C/min.Det.: FID @ 350°C



GC_EV00595

Polynuclear Aromatic HydrocarbonsUS EPA Method 610Rtx®-5Sil MS

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Polynuclear Aromatic HydrocarbonsUS EPA Method 610Rtx®-5

0.53mm ID(direct injection)

30m, 0.53mm ID, 1.50µm Rtx®-5 (cat.# 10270)2.5µL direct injection of PAHs using a Uniliner®.Concentration approximately 25ng per component.



min. 4 8 12 16 20 24 28 32 36 40 44

1415

16

13

1112

12 3 4 5

6

78

109

GC_EV00043

Reference StandardSV Calibration Mix #5 (cat.# 31011):

1. naphthalene2. acenaphthylene3. acenapthene4. fluorene5. phenanthrene6. anthracene7. fluoranthene8. pyrene9. benzo(a)anthracene


0.32mm ID (cold on-column injection)

30m, 0.32mm ID, 0.25µm Rtx®-5 (cat.# 10224)0.5µL cold on-column injection of PAHs.Concentration approximately 10ng per component.

Oven temp.: 35°C (hold 4 min.) to 325°C @ 10°C/min. (hold)

Det. temp.: 325°CCarrier gas: hydrogenLinear velocity: 40cm/sec.FID sensitivity: 8 x 10-11 AFS

GC_EV00376

min. 4 8 12 16 20 24 28 32

1

23

465

7 8 10

913

11

12

1615

14

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min. 4 5 6 7 8 9 10


1

23

4

5

6 78

9

1011

1213 14,15

1. naphthalene2. acenaphthylene3. acenaphthene4. fluorene5. phenanthrene6. anthracene7. fluoranthene8. pyrene9. chrysene

10. benzo(a)anthracene11. benzo(b)fluoranthene12. benzo(k)fluoranthene13. benzo(a)pyrene14. indeno(1,2,3-cd)pyrene15. dibenzo(a,h)anthracene16. benzo(ghi)perylene

10m, 0.10mm ID, 0.10µm Rtx®-5 (cat.# 41201)0.5µL splitless injection. 41psi initial pressure, hold 2 min.8 psi/min. to 99psi (hold 1.87 min.). 275°C, vent open @ 1 min. Oven temp.: 40°C (hold 0.5 min.) to 90°C @ 70°C/min. then

to 100°C @ 5°C/min., to 310°C @ 30°C/min. (hold 2 min.).

Fast GC usingmicrobore column

GC_EV00045

16


Column: Rtx®-440 30m, 0.25mm ID, 0.25µm (cat.# 12923)Sample: 610 PAH Mix (cat.# 31011) diluted to 20ppm each compound in methylene chlorideInj.: 1.0µL splitless (hold 0.4 min.), 4mm splitless liner (cat.# 20772)Inj. temp.: 320°CCarrier gas: hydrogen, constant flowFlow: 3.6mL/min.Oven temp.: 40°C (hold 2 min.) to 240°C @ 30°C/min., to 320°C @ 8°C/min. (hold 5 min.)Det.: FID @ 320°C



GC_EV00732


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Chlorinated HydrocarbonsUS EPA Method 612Rtx®-200

30m, 0.53mm ID, 0.50µm Rtx®-200 (cat.# 15040)0.5µL direct injection of chlorinated hydrocarbons.


Inj. / det. temp.: 275°C / 220°CCarrier gas: heliumLinear velocity: 40cm/sec. set @ 40°C

1

5

7

8

Reference Standard612 Chlorinated Hydrocarbons Mix (cat.# 31035):

1. 1,3-dichlorobenzene (0.2mg/mL)2. 1,4-dichlorobenzene (0.3mg/mL)3. 1,2-dichlorobenzene (0.3mg/mL)4. hexachloroethane (0.02mg/mL)5. 1,2,4-trichlorobenzene (0.2mg/mL)6. hexachlorobutadiene (0.02mg/mL)7. hexachlorocyclopentadiene (0.02mg/mL)8. 2-chloronaphthalene (0.3mg/mL)9. hexachlorobenzene (0.02mg/mL)

min. 4 8 12 16

GC_EV00051

96

3

2,4

HaloethersUS EPA Method 611Rtx®-5

4

5

15m, 0.53mm ID, 1.50µm Rtx®-5 (cat.# 10267)0.25µL split injection of EPA Method 611 haloethers.On-column concentration 28ng/component.

Oven temp.: 100°C to 300°C @ 15°C/min. (hold 10 min.)Inj. / det. temp.: 200°C / 300°CCarrier gas: heliumLinear velocity: 23cm/sec. set @ 40°C

Reference Standard611 Haloethers Mix (cat.# 31034):

1. bis(2-chloroethyl)ether2. bis(2-chloroisopropyl)ether3. bis(2-chloroethoxy)methane4. 4-chlorophenyl phenyl ether5. 4-bromophenyl phenyl ether

21

3

GC_EV00050min. 4 8 12 16

2

Polynuclear Aromatic HydrocarbonsUS EPA Method 8100MXT®-5

30m, 0.28mm ID, 0.25µm MXT®-5 (cat.# 70224)1.0µL splitless injection of PAHs.Concentration 20ng/µL.

Oven temp.: 40°C to 325°C @ 10°C/min. (hold 10 min.) Inj. / det. temp.: 280°C / 325°CDetector: MSCarrier gas: heliumLinear velocity: 50cm/sec. set @ 325°CSplitless hold time: 1 min.

Reference StandardsSV Calibration Mix #5 (cat.# 31011):



min. 6 8 10 12 14 16 18 20 22 24 26 28

1

4

56

9 10 11

12

13

1615

3

8

GC_EV00042

14

7

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Chlorinated HydrocarbonsUS EPA Method 612MXT®-5

1. 1,3-dichlorobenzene2. 1,4-dichlorobenzene3. 1,2-dichlorobenzene4. hexachloroethane5. 1,2,4-trichlorobenzene6. hexachlorobutadiene7. hexachlorocyclopentadiene8. 2-chloronaphthalene9. hexachlorobenzene

2

1

3

4 5 67

9

8

min. 4 8 12 16 20

GC_EV00054

30m, 0.53mm ID, 0.50µm MXT®-5 (cat.# 70240)0.2µL direct injection of chlorinated hydrocarbons.

Oven temp.: 40°C to 280°C @ 8°C/min. (hold 15 min.)Inj. / det. temp.: 200°C / 300°CCarrier gas: heliumLinear velocity: 20cm/sec. (flow rate: 2.5cc/min.)

1. aniline2. benzyl alcohol3. 2-methylphenol4. 4-methylphenol5. benzoic acid6. 4-chloroaniline7. 2-methylnaphthalene8. 2,4,5-trichlorophenol9. 2-nitroaniline

10. dibenzofuran11. 3-nitroaniline12. 4-nitroaniline

US EPA Hazardous SubstancesRtx®-5

30m, 0.53mm ID, 1.50µm Rtx®-5 (cat.# 10270)2.5µL direct injection of hazardous substances using a Uniliner® liner.Concentration approximately 25ng per component.


Inj. & det. temp.: 300°CCarrier gas: hydrogenLinear velocity: 80cm/sec. (flow rate: 10cc/min.) set @ 40°C

1 2 4

3

5

6

8

10

9

12

117

GC_EV00055

min. 4 8 12 16 20 24 28 32 36

Reference Standard612 Chlorinated Hydrocarbons Mix (cat.# 31035):

1. 1,3-dichlorobenzene (0.2mg/mL)2. 1,4-dichlorobenzene (0.3mg/mL)3. 1,2-dichlorobenzene (0.3mg/mL)4. hexachloroethane (0.02mg/mL)5. 1,2,4-trichlorobenzene (0.2mg/mL)6. hexachlorobutadiene (0.02mg/mL)7. hexachlorocyclopentadiene (0.02mg/mL)8. 2-chloronaphthalene (0.3mg/mL)9. hexachlorobenzene (0.02mg/mL)

0.1µL direct injection of chlorinated hydrocarbons. Concentration: 4ng/µLOven temp.: 40°C to 250°C @ 8°C/min. (hold 5 min.)Inj. / det. temp.: 250°C / 300°CCarrier gas: heliumLinear velocity: 35cm/sec. set @ 40°C


30m, 0.53mm ID, 0.50µm Rtx®-35 (cat.# 10440)

min. 5 10 15 20

GC_EV00052

2

1 3

9

8

75,64


97654

8

31

2

30m, 0.53mm ID, 0.50µm Rtx®-5 (cat.# 10240)

GC_EV00053

0.1µL direct injection of chlorinated hydrocarbons. Concentration: 4ng/µLOven temp.: 40°C to 250°C @ 8°C/min. (hold 5 min.)Inj. / det. temp.: 250°C / 300°CCarrier gas: heliumLinear velocity: 35cm/sec. set @ 40°C

min. 5 10 15 20

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Semivolatile OrganicsUS EPA Method 526 (Screening)Rtx®-5Sil MS

GC_EV00656

Rtx®-5Sil MS, 30m, 0.25mm ID, 0.25µm (cat.# 12723)US EPA Method 526 Mix 1µL 10ppm (20ppm IS) Primary Dilution Standard / Semivolatiles/ EPA 526 cat.# 31691Surrogate Standard / Method 526 cat.# 31693Internal Standard / Method 526 cat.# 31692

Inj.: 1.0µL splitless (hold 0.3 min.), 4mm Drilled Uniliner® (cat.# 21055)

GC Agilent 6890 Inj. temp.: 300°CCarrier gas: helium, constant flowFlow rate: 0.8 mL/min.Oven temp.: 50°C (hold 1 min.) to 200°C @ 20°C/min. (hold 5 min.)

to 310°C @ 30°C/min. (hold 3 min.)Det: Agilent 5973 GC/MSTransfer line temp.: 280°CScan range: 35-550 amuSolvent delay: 5.5 min.Tune: DFTPPIonization: EI

1. nitrobenzene2. 2,4-dichlorophenol3. 1,3-dimethyl-2-nitrobenzene (ss)4. 2,4,6-trichlorophenol5. acenaphthene-d10 (IS#1)6. azobenzene*7. prometon8. terbufos9. diazinon

10. fonofos

11. phenanthrene-d10 (IS#2)12. disulfoton13. acetochlor14. cyanazine15. triphenylphosphate (ss)16. chrysene-d12* Compound listed in method,

1,2-diphenylhydrazine, decomposes toazobenzene.

Rtx®-5Sil MS 30m, 0.25mm ID, 0.25µm (cat.# 12723)US EPA Method 528 Mix 1µL 5ppm standard Phenol Stock Calibration Solution cat.# 31694Internal Standard Phenols Method 528 cat.# 31696Surrogate Fortification Standard/Phenols cat.# 316925ng each analyte on-column

Inj.: 1.0µL pulsed splitless (hold 0.5 min.),4mm Drilled Uniliner® (cat.# 21055), pulsed pressure 50psi for 0.5 min.

GC: Agilent 6890 Inj. temp.: 220°CCarrier gas: helium, constant flow Flow rate: 1.3 mL/min. Oven temp.: 40°C (hold 1 min.) to 220°C @ 12°C/min.

(hold 0 min.) to 300°C @ 30°C/min (hold 1 min.) Det: Agilent 5973 GC/MSTransfer line temp.: 280°CScan range: 35-550 amuSolvent delay: 5.5 min.Tune: DFTPPIonization: EI

PhenolsUS EPA Method 528Rtx®-5Sil MS

GC_EV00664

1. phenol2. 2-chlorophenol-3,4,5,6-d4 (SS)3. 2-chlorophenol4. 2-methylphenol5. 2-nitrophenol6. 2,4-dimethylphenol-3,5,6-d3 (SS)7. 2,4-dimethylphenol8. 2,4-dichlorophenol9. 4-chloro-3-methylphenol

10. 1,2-dimethyl-3-nitrobenzene (IS#1)11. 2,4,6-trichlorophenol12. 2,4-dinitrophenol13. 4-nitrophenol14. 2,3,4,5-tetrachlorophenol (IS#2)15. 2-methyl-4,6-dinitrophenol16. 2,4,6-tribromophenol (SS)17. pentachlorophenol

Optimizedresolution!

Excellent sensitivity and peak shape

at 5ng on-column

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RT1. 2-fluorophenol (SS) 4.652. phenol-d6 (SS) 5.533. phenol 5.554. bis(2-chloroethyl)ether 5.645. 2-chlorophenol-d4 (SS) 5.666. 2-chlorophenol 5.677. 1,3-dichlorobenzene 5.818. 1,4-dichlorobenzene-d4 (IS) 5.869. 1,4-dichlorobenzene 5.87

10. 1,2-dichlorobenzene-d4 (IS) 6.0111. benzyl alcohol 6.0112. 1,2-dichlorobenzene 6.0313. 2-methylphenol 6.1014. bis(2-chloroisopropyl)ether 6.1315. 4-methylphenol/3-methylphenol 6.2516. N-nitroso-di-n-propylamine 6.2817. hexachloroethane 6.3318. nitrobenzene-d5 (SS) 6.4119. nitrobenzene 6.4320. isophorone 6.6621. 2-nitrophenol 6.7322. 2,4-dimethylphenol 6.7523. bis(2-chloroethoxy)methane 6.8524. benzoic acid 6.9125. 2,4-dichlorophenol 6.9526. 1,2,4-trichlorobenzene 7.0327. naphthalene-d8 (IS) 7.08

28. naphthalene 7.1029. 4-chloroaniline 7.2030. hexachlorobutadiene 7.2131. 4-chloro-3-methylphenol 7.6232. 2-methylnaphthalene 7.7733. hexachlorocyclopentadiene 7.9234. 2,4,6-trichlorophenol 8.0535. 2,4,5-trichlorophenol 8.0836. 2-fluorobiphenyl (SS) 8.1337. 2-chloronaphthalene 8.2538. 2-nitroaniline 8.3839. dimethylphthalate 8.5440. 2,6-dinitrotoluene 8.6241. acenaphthylene 8.6642. acenaphthene-d10 (IS) 8.7943. acenaphthene 8.8344. 3-nitroaniline 8.8845. 2,4-dinitrophenol 8.8846. 4-nitrophenol 8.9247. dibenzofuran 8.9948. 2,4-dinitrotoluene 9.0149. diethyl phthalate 9.2250. 4-chlorophenyl phenyl ether 9.3251. fluorene 9.3352. 4-nitroaniline 9.3953. 2-methyl-4,6-dinitrophenol 9.4054. diphenylamine 9.4455. 2,4,6-tribromophenol (SS) 9.57

56. 4-bromophenyl phenyl ether 9.8057. hexachlorobenzene 9.8858. pentachlorophenol 10.0759. phenanthrene-d10 (IS) 10.2460. phenanthrene 10.2761. anthracene 10.3262. carbazole 10.4963. di-n-butylphthalate 10.8464. fluoranthene 11.6265. pyrene 11.9466. p-terphenyl-d14 (SS) 12.1667. butyl benzyl phthalate 13.0168. benzo(a)anthracene 14.0569. 3,3'-dichlorobenzidine 14.0670. chrysene-d12 (IS) 14.0871. chrysene 14.1472. bis(2-ethylhexyl)phthalate 14.273. di-n-octyl phthalate 15.7774. benzo(b)fluoranthene 16.5775. benzo(k)fluoranthene 16.6476. benzo(a)pyrene 17.3877. perylene-d12 (IS) 17.5278. indeno(1,2,3-cd)pyrene 20.1879. dibenzo(a,h)anthracene 20.2680. benzo(ghi)perylene 20.81

GC_EV00544

30m, 0.25 mm ID, 0.25µm Rtx®-5MS (cat.# 12623)80ng splitless injection of CLP standard

Oven temp.: 40°C (hold 2 min.) to 245°C @ 25°C/min.to 330°C @6°C/min. (hold 5 min.)

Carrier gas: helium @ 1.0 mL/min. constant flowInj. temp.: 300°CDet. temp.: 280°CScan range: 35-550 amu

SemivolatilesCLP MethodRtx®-5MS

• 80ng splitless• 0.25µm film thickness• 5% diphenyl phase

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RT1. N-nitrosodimethylamine 3.382. pyridine 3.373. methyl methanesulfonate 4.534. 2-fluorophenol (SS) 4.615. ethyl methanesulfonate 5.166. phenol 5.517. phenol-d6 (SS) 5.508. aniline 5.529. bis(2-chloroethyl)ether 5.58

10. 2-chlorophenol-d4 (SS) 5.6011. 2-chlorophenol 5.6112. 1,3-dichlorobenzene 5.7313. 1,4-dichlorobenzene-d4 (IS) 5.7914. 1,4-dichlorobenzene 5.8015. benzyl alcohol 5.9616. 1,2-dichlorobenzene-d4 (SS) 5.9417. 1,2-dichlorobenzene 5.9618. 2-methylphenol 6.0619. bis(2-chloroisopropyl)ether 6.06

20a. 4-methylphenol 6.2220b. 3-methylphenol 6.22

21. N-nitroso-di-n-propylamine 6.2422. acetophenone 6.2023. hexachloroethane 6.2524. nitrobenzene-d5 (SS) 6.3525. nitrobenzene 6.3726. isophorone 6.5927. 2,4-dimethylphenol 6.7028. 2-nitrophenol 6.6629. benzoic acid 6.8730. bis(2-chloroethoxy)methane 6.7931. 2,4-dichlorophenol 6.8932. 1,2,4-trichlorobenzene 6.9533. naphthalene-d8 (IS) 7.0034. naphthalene 7.03

35. 2,6-dichlorophenol 7.1036. 4-chloroaniline 7.1237. hexachloropropene 7.1038. hexachlorobutadiene 7.1439. 4-chloro-3-methylphenol 7.5740. isosafrole 7.6141. 2-methylnaphthalene 7.6942. 1-methylnaphthalene 7.7943. hexachlorocyclopentadiene 7.8544. 1,2,4,5-tetrachlorobenzene 7.8645. 2,4,6-trichlorophenol 7.9846. 2,4,5-trichlorophenol 8.0247. 2-fluorobiphenyl (SS) 8.0548. safrole 8.1149. 2-chloronaphthalene 8.1750. 2-nitroaniline 8.3251. 1,4-naphthoquinone 8.3752. dimethylphthalate 8.4853. 1,3-dinitrobenzene 8.5454. 2,6-dinitrotoluene 8.5655. acenaphthylene 8.5756. 3-nitroaniline 8.6857. acenaphthene-d10 (IS) 8.7158. acenaphthene 8.7459. 2,4-dinitrophenol 8.8260. 4-nitrophenol 8.9161. pentachlorobenzene 8.8962. 2,4-dinitrotoluene 8.9463. dibenzofuran 8.9164. 2,3,4,6-tetrachlorophenol 9.0565. diethyl phthalate 9.1566. 4-chlorophenyl phenyl ether 9.2467. fluorene 9.2468. 4-nitroaniline 9.3069. 4,6-dinitro-2-methylphenol 9.3370. diphenylamine 9.37

71. azobenzene 9.3972. 2,4,6-tribromophenol (SS) 9.4973. phenacetin 9.7074. 4-bromophenyl phenyl ether 9.7175. hexachlorobenzene 9.8076. pentachlorophenol 9.9977. pentachloronitrobenzene 10.0278. dinoseb 10.1679. phenanthrene-d10 (IS) 10.1680. phenanthrene 10.1881. anthracene 10.2382. di-n-butylphthalate 10.7583. 4-nitroquinoline-1-oxide 11.0484. isodrin 11.2985. fluoranthene 11.5086. benzidine 11.7187. pyrene 11.8088. Aramite 12.1089. p-terphenyl-d14 (SS) 12.0290. chlorbenzilate 12.3191. butyl benzyl phthalate 12.8492. Kepone 12.8193. bis(2-ethylhexyl)phthalate 13.9994. chrysene-d12 (IS) 13.8795. 3,3'-dichlorobenzidine 13.8796. benzo(a)anthracene (IS) 13.8497. chrysene 13.9398. di-n-octyl phthalate 15.5199. benzo(b)fluoranthene 16.29

100. benzo(k)fluoranthene 16.35101. benzo(a)pyrene 17.07102. perylene-d12 (IS) 17.22103. 3-methylcholanthrene 17.98104. indeno(1,2,3-cd)pyrene 19.82105. dibenzo(a,h)anthracene 19.91106. benzo(ghi)perylene 20.44

GC_EV00545

30m, 0.25 mm ID, 0.25µm Rtx®-5Sil MS (cat.# 12723)80ng splitless injection of 8270 standard


Carrier gas: helium @ 1.0mL/min. constant flowInj. temp.: 300°CDet. temp.: 280°CScan range: 35-550amu

SemivolatilesUS EPA Method 8270Rtx®-5Sil MS

• 80ng splitless• 0.25µm film thickness• Silarylene phase

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Semivolatile OrganicsUS EPA Method 8270Rtx®-5Sil MS

1. N-nitrosodimethylamine2. pyridine3. methyl methanesulfonate4. 2-fluorophenol5. ethyl methanesulfonate6. phenol-d67. phenol8. aniline9. bis(2-chloroethyl)ether

10. 2-chlorophenol-d411. 2-chlorophenol12. 1,3-dichlorobenzene13. 1,4-dichlorobenzene-d414. 1,4-dichlorobenzene15. benzyl alcohol16. 1,2-dichlorobenzene-d417. 1,2-dichlorobenzene18. 2-methylphenol19. bis(2-chloroisopropyl)ether

20a. 4-methylphenol20b. 3-methylphenol21. N-nitroso-di-n-propylamine

22. acetophenone23. hexachloroethane24. nitrobenzene-d525. nitrobenzene26. isophorone27. 2,4-dimethylphenol28. 2-nitrophenol29. benzoic acid30. bis(2-chloroethoxy)methane31. 2,4-dichlorophenol32. 1,2,4-trichlorobenzene33. naphthalene-d834. naphthalene35. 2,6-dichlorophenol36. 4-chloroaniline37. hexachloropropene38. hexachlorobutadiene39 4-chloro-3-methylphenol40. isosafrole41. 2-methylnaphthalene42. 1-methylnaphthalene43. hexachlorocyclopentadiene

44. 1,2,4,5-tetrachlorobenzene45. 2,4,6-trichlorophenol46. 2,4,5-trichlorophenol47. 2-fluorobiphenyl48. safrole49. 2-chloronaphthalene50. 2-nitroaniline51. 1,4-naphthoquinone52. dimethylphthalate53. 1,3-dinitrobenzene54. 2,6-dinitrotoluene55. acenaphthylene56. 3-nitroaniline57. acenaphthene-d1058. acenaphthene59. 2,4-dinitrophenol60. 4-nitrophenol61. pentachlorobenzene62. 2,4-dinitrotoluene63. dibenzofuran64. 2,3,4,6-tetrachlorophenol65. diethyl phthalate

66. 4-chlorophenyl phenyl ether67. fluorene68. 4-nitroaniline69. 4,6-dinitro-2-methylphenol70. diphenylamine71. azobenzene72. 2,4,6-tribromophenol73. phenacetin74. 4-bromophenyl phenyl ether75. hexachlorobenzene76. pentachlorophenol77. pentachloronitrobenzene78 dinoseb79. phenanthrene-d1080. phenanthrene81. anthracene82. di-n-butylphthalate83. 4-nitroquinoline-1-oxide84. isodrin85. fluoranthene86. benzidine87. pyrene

88. Aramite89. p-terphenyl-d1490. chlorbenzilate91. butyl benzyl phthalate92. Kepone93. bis(2-ethylhexyl)phthalate94. 3,3'-dichlorobenzidine95. benzo(a)anthracene96. chrysene-d1297. chrysene98. di-n-octyl phthalate99. benzo(b)fluoranthene

100. benzo(k)fluoranthene101. benzo(a)pyrene102. perylene-d12103. 3-methylcholanthrene104. indeno(1,2,3-cd)pyrene105. dibenzo(a,h)anthracene106. benzo(ghi)perylene

1,2

3

4

5

6,7

10,11

34

47

58

67

81

82

83

84

85

86

87,88

89

90 91

92

93

94

95,9697

98 99100

101

102 103

104,105 106

6,7

8

9

10,11

12

13,14

15

16,17

18

19

20 21,22

2324

25 26

27,28

29,30

31

32

33

34

35,36

37

38

39

40

4142

44

43

45

46

47

48

49

50

51 52

53

54

55

57

58

6059

61

62,63

64

65

66

6768,69

7071

72

73

74 75

76

77

7879

8081

30m, 0.25mm ID, 0.5µmRtx®-5Sil MS (cat# 12738)80ng splitless injection of 8270 standard GC: Agilent 6890Det.: MSDOven temp.: 40°C (hold 2 min) to 290°C

@ 20°C/min. (hold 0 min.) to 303°C @ 2°C/min. (hold 0 min.) to 330°C @6°C/min. (hold 1 min.)

Carrier gas: helium @ 1.0mL/min. constant flow

Inj. temp.: 300°CDet. temp.: 280°C, scan range 35-550 AMU

min. 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

min. 7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5 11.0 11.5 12.0 12.5

GC_EV00532

Excellent benzo(b) & benzo(k)fluoranthene separation!

• 80ng splitless• 0.5µm film thickness• Silarylene phase

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Semivolatiles558


Rtx®-5Sil MS, 30m, 0.25mm ID, 0.25µm (cat.# 12723)Sample: 16µg/mL in methylene chloride Inj.: 1µL splitless (hold 0.4 min.), Drilled Uniliner® liner (cat.# 21054) Inj. temp.: 300°CCarrier gas: helium, constant flowFlow rate: 1mL/min.Oven temp.: 35°C (hold 2 min.) to 260°C @ 20°C/min., to 330°C @ 6°C/min. (hold 1 min.)Det.: MSTransfer line temp.: 280°CScan range: 35–550amuIonization: EIMode: full scan

Analytical Reference Materials used: cat.# 31618, 31619, 31620, 31621, 31622, 31206, 31062, 31063


10. 2-chlorophenol11. 1,3-dichlorobenzene12. 1,4-dichlorobenzene-d413. 1,4-dichlorobenzene14. 1,2-dichlorobenzene15. benzyl alcohol16. 2-methylphenol17. bis(2-chloroisopropyl)ether18. acetophenone

19a. 4-methylphenol19b. 3-methylphenol 20. N-nitroso-di-n-propylamine21. hexachloroethane22. nitrobenzene-d523. nitrobenzene24. isophorone25. 2-nitrophenol26. 2,4-dimethylphenol

27. bis(2-chloroethoxy)methane28. benzoic acid29. 2,4-dichlorophenol30. 1,2,4-trichlorobenzene31. naphthalene-d832. naphthalene33. 2,6-dichlorophenol34. 4-chloroaniline35. hexachloropropene36. hexachlorobutadiene37. 4-chloro-3-methylphenol38. isosafrole39. 2-methylnaphthalene40. 1-methylnaphthalene41. hexachlorocyclopentadiene42. 1,2,4,5-tetrachlorobenzene43. 2,4,6-trichlorophenol44. 2,4,5-trichlorophenol45. 2-fluorobiphenyl46. safrole47. 2-chloronaphthalene48. 2-nitroaniline49. 1,4-naphthoquinone50. dimethyl phthalate51. 1,3-dinitrobenzene52. 2,6-dinitrotoluene53. acenaphthylene

54. acenaphthene-d1055. 3-nitroaniline56. acenaphthene57. 2,4-dinitrophenol58. pentachlorobenzene59. 4-nitrophenol60. dibenzofuran61. 2,4-dinitrotoluene62. 2,3,4,6-tetrachlorophenol63. diethyl phthalate64. fluorene65. 4-chlorophenyl phenyl ether66. 4-nitroaniline67. 4,6-dinitro-2-methylphenol68. diphenylamine69. azobenzene70. 2,4,6-tribromophenol71. phenacetin72. 4-bromophenyl phenyl ether73. hexachlorobenzene74. pentachlorophenol75. pentachloronitrobenzene76. phenanthrene-d1077. dinoseb78. phenanthrene79. anthracene80. di-n-butylphthalate

81. 4-nitroquinoline-1-oxide82. isodrin83. fluoranthene84. benzidine85. pyrene 86. p-terphenyl-d1487. Aramite88. chlorbenzilate89. Kepone90. butyl benzyl phthalate91. benzo(a)anthracene92. 3,3'-dichlorobenzidine93. chrysene-d1294. chrysene95. bis(2-ethylhexyl)phthalate96. di-n-octyl phthalate97. benzo(b)fluoranthene98. benzo(k)fluoranthene99. benzo(a)pyrene

100. perylene-d12101. 3-methylcholanthrene102. indeno(1,2,3-cd)pyrene103. dibenzo(a,h)anthracene104. benzo(ghi)perylene

GC_EV00561


• 16ng on-column• Drilled Uniliner® inlet liner• 0.25µm film thickness• Silarylene phase

See page 559 for resolution of close eluting compounds.

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1. phenol (m/z 94)2. aniline (m/z 93) 3. bis(2-chloroethyl)ether (m/z 93)

GC_EV00562

1. 1,3-dichlorobenzene (m/z 146)2. 1,4-dichlorobenzene (m/z 146)3. 1,2-dichlorobenzene (m/z 146)

GC_EV00563

1. benzo(b)fluoranthene (m/z 252)2. benzo(k)fluoranthene (m/z 252)

GC_EV00564

Rtx®-5Sil MS, 30m, 0.25mm ID, 0.25µm (cat.# 12723)Sample: 16µg/mL in methylene chloride Inj.: 1µL splitless (hold 0.4 min.),

Drilled Uniliner® liner (cat.# 21054) Inj. temp.: 300°CCarrier gas: 1mL/min., constant flowLinear velocity: 34cm/sec.Oven temp.: 35°C (hold 2 min.) to 260°C @ 20°C/min., to

330°C @ 6°C/min. (hold 1 min.)Det.: MSTransfer line temp.: 280°CScan range: 35–550amuIonization: EIMode: full scan

1. indeno(1,2,3-cd)pyrene (m/z 276)2. dibenzo(a,h)anthracene (m/z 278)

GC_EV00565

please notehighlighted regions fromc-grams on ppaaggee 555588.

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Rtx®-5Sil MS with 5 meter Integra-Guard™integral guard column, 30m, 0.25mm ID, 0.25µm (cat.# 12723-127)Sample: US EPA Method 8270 Mix 1µL 10 ppm (10ng on column)

8270 Calibration Mix #1, cat.# 316188270 Calibration Mix #2, cat.# 316198270 Calibration Mix #3, cat.# 316208270 Calibration Mix #4, cat.# 316218270 Calibration Mix #5, cat.# 31622SV Internal Standard Mix, cat.# 31206Acid Surrogate Mix (4/89), cat.# 31063B/N Surrogate Standard Mix (4/89), cat.# 31062

Inj.: 1.0µL splitless (hold 0.3 min.), 4mm Drilled Uniliner® (cat.# 21055)Inj. temp.: 300°CCarrier gas: helium, constant flow Flow rate: 1.1mL/min. Dead Time: 1.8 minutes @ 35°C


10. 2-chlorophenol-d411. 1,3-dichlorobenzene12. 1,4-dichlorobenzene-d413. 1,4-dichlorobenzene14. benzyl alcohol15. 1,2-dichlorobenzene16. 2-methylphenol17. bis(2-chloroisopropyl)ether18. 4-methylphenol/3-methylphenol 19. acetophenone20. N-nitroso-di-n-propylamine21. hexachloroethane22. nitrobenzene-d5

23. nitrobenzene24. isophorone25. 2-nitrophenol26. 2,4-dimethylphenol27. benzoic acid28. bis(2-chloroethoxy)methane29. 2,4-dichlorophenol30. 1,2,4-trichlorobenzene31. naphthalene-d832. naphthalene33. 2,6-dichlorophenol34. 4-chloroaniline35. hexachloropropene36. hexachlorobutadiene37. 4-chloro-3-methylphenol38. isosafrole39. 2-methylnaphthalene40. 1-methylnaphthalene41. hexachlorocyclopentadiene42. 1,2,4,5-tetrachlorobenzene43. 2,4,6-trichlorophenol44. 2,4,5-trichlorophenol

45. 2-fluorobiphenyl46. safrole47. 2-chloronaphthalene48. 2-nitroaniline49. 1,4-naphthoquinone50. dimethyl phthalate51. 1,3-dinitrobenzene52. 2,6-dinitrotoluene53. acenaphthylene54. 3-nitroaniline55. acenaphthene-d1056. acenaphthene57. 2,4-dinitrophenol58. 4-nitrophenol59. pentachlorobenzene60. dibenzofuran61. 2,4-dinitrotoluene62. 2,3,4,6-tetrachlorophenol63. diethyl phthalate64. 4-chlorophenyl phenyl ether65. fluorene66. 4-nitroaniline

67. 4,6-dinitro-2-methylphenol68. diphenylamine69. azobenzene70. 2,4,6-tribromophenol71. phenacetin72. 4-bromophenyl phenyl ether73. hexachlorobenzene74. pentachlorophenol75. pentachloronitrobenzene76. dinoseb77. phenanthrene-d1078. phenanthrene79. anthracene80. di-n-butyl phthalate81. 4-nitroquinoline-1-oxide82. isodrin83. fluoranthene84. benzidine85. pyrene86. aramite87. p-terphenyl-d1488. aramite isomer

89. chlorbenzilate90. butyl benzyl phthalate91. kepone92. benzo(a)anthracene93. 3,3'-dichlorobenzidine94. chrysene-d1295. chrysene96. bis(2-ethylhexyl) phthalate97. di-n-octyl phthalate98. benzo(b)fluoranthene99. benzo(k)fluoranthene

100. benzo(a)pyrene101. perylene-d12102. 3-methylcholanthrene103. indeno(1,2,3-cd)pyrene104. dibenzo(a,h)anthracene105. benzo(ghi)perylene

Oven temp.: 35°C (hold 4 min.) to 245°C @ 25°C/min.(no hold) to 330°C @ 6°C/min. (hold 3 min.)

Det: Agilent 5973 GC/MSTransfer line temp.: 280°CScan range: 35–550 amuSolvent delay: 5 min.Tune: DFTPPIonization: EI

GC_EV00611

GC_EV00612

• 10ng on-column• Drilled Uniliner® inlet liner• 0.25µm film thickness• Silarylene phase• Integra-Guard™ column

Semivolatile OrganicsUS EPA Method 8270Rtx®-5Sil MS(Integra-Guard™)

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SemivolatileOrganicsUS EPAAppendix IXRtx®-5Sil MS

1. 1,4-dioxane2. pyridine3. N-nitrosodimethylamine4. ethyl methacrylate5. 2-picoline6. N-nitrosomethylethylamine7. methyl methanesulfonate8. 2-fluorophenol9. N-nitrosodiethylamine

10. ethyl methanesulfonate11. benzaldehyde12. phenol-d6 (SS)13. phenol14. aniline15. pentachloroethane16. bis(2-chloroethyl)ether17. 2-chlorophenol18. 1,3-dichlorobenzene19. 1,4-dichlorobenzene-d4

(IS)20. 1,4-dichlorobenzene21. benzyl alcohol22. 1,2-dichlorobenzene23. 2-methylphenol

24. bis(2-chloroisopropyl)ether25. acetophenone

26a. 4-methylphenol26b. 3-methylphenol27. N-nitroso-di-n-propylamine28. nitrosopyrrolydine29. o-toluidine30. 4-nitrosomorpholine31. hexachloroethane32. nitrobenzene-d5 (SS)33. nitrobenzene34. N-nitrosopiperidine35. isophorone36. 2-nitrophenol37. 2,4-dimethylphenol38. bis(2-chloroethoxy)methane39. 2,4-dichlorophenol40. 1,2,4-trichlorobenzene41. α,α-dimethylphenylamine42. naphthalene-d8 (IS)43. naphthalene44. 2,6-dichlorophenol45. 4-chloroaniline46. hexachloropropene

47. hexachlorobutadiene48. N-nitroso-n-butylamine49. 1,4-phenylenediamine50. caprolactam51. 4-chloro-3-methylphenol52. isosafrole53. 2-methylnaphthalene54. 1-methylnaphthalene55. hexachlorocyclopentadiene56. 1,2,4,5-tetrachlorobenzene57. isosafrole58. 2,4,6-trichlorophenol59. 2,4,5-trichlorophenol60. 2-fluorobiphenyl (SS)61. safrole62. biphenyl63. 2-chloronaphthalene64. 1-chloronaphthalene65. diphenyl ether66. 2-nitroaniline67. 1,4-naphthoquinone68. 1,4-dinitrobenzene69. dimethylphthalate70. 1,3-dinitrobenzene

71. 2,6-dinitrotoluene72. acenaphthylene73. 1,2-dinitrobenzene74. 3-nitroaniline75. acenaphthene-d10 (IS)76. acenaphthene77. 2,4-dinitrophenol78. pentachlorobenzene79. 4-nitrophenol80. dibenzofuran81. 2,4-dinitrotoluene82. 1-naphthalamine83. 2,3,4,6-tetrachlorophenol84. 2,3,5,6-tetrachlorophenol85. 2-naphthylamine86. diethyl phthalate87. fluorene88. 4-chlorophenyl phenyl ether89. 2-methyl-5-nitroaniline90. 4-nitroaniline91. 4,6-dinitro-2-methylphenol92. diphenylamine93. azobenzene94. 2,4,6-tribromophenol (SS)

95. diallate96. 1,3,5-trinitrobenzene97. phenacetin98. 4-bromophenyl phenyl ether99. hexachlorobenzene

100. atrazine101. 4-aminobiphenyl102. pentachlorophenol103. pentachloronitrobenzene104. propyzamide105. phenanthrene-d10 (IS)106. phenanthrene107. anthracene108. carbazole109. di-n-butylphthalate110. 4-nitroquinoline-1-oxide111. methapyriline112. isodrin113. fluoranthene114. pyrene115. Aramite (isomer)116. p-terphenyl-d14 (SS)117. Aramite (isomer)118. dimethylaminoazobenzene

119. dichlorobenzilate120. 3,3-dimethylbenzidine121. butyl benzyl phthalate122. Kepone123. bis(2-ethylhexyl)adipate124. 2-acetylaminofluorene125. benz(a)anthracene126. chrysene-d12 (IS)127. chrysene128. bis(2-ethylhexyl)phthalate129. di-n-octyl phthalate130. benzo(b)fluoranthene131. 7,12-dimethylbenzo(a)anthracene132. benzo(k)fluoranthene133. benzo(a)pyrene134. perylene-d12 (IS)135. 3-methylcholanthrene136. dibenzo(a,j)acridine 137. indeno(1,2,3-cd)pyrene138. dibenzo(a,h)anthracene139. benzo(ghi)perylene

GC_EV00682

Column: Rtx®-5Sil MS, 30m, 0.25mm ID, 0.25µm (cat.# 12723)

Sample:8270 MegaMix™ (cat.# 31686)Appendix IX Mix #1 (cat#31625)Appendix IX Mix #2 (cat#31806)SV Internal Standard Mix (cat#31206)B/N Surrogate Mix (4/89 SOW) (cat#31062)Acid Surrogate Mix (4/89 SOW) (cat#31063)50ng each analyte on-column

Inj.: 1.0µL splitless, double gooseneck inlet liner (cat.# 20784)pressure pulse injection, 30psig (0.5min)

Inj. temp.: 275°CCarrier gas: helium, constant flowColumn flow: 1mL/minOven temp.: 35°C (hold 1 min.) to 260°C @ 20°C/min. to 330°C @ 6°C/min (hold 1 min.)Det.: 5973 MSTransfer line temp.: 280°CScan range: 35-550amuIonization: EIMode: TIC

• 50ng each on-column• 0.25µm film thickness• Silarylene phase

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Semivolatile OrganicsUS EPA OLC 03.2Rtx®-5Sil MS

Rtx®-5Sil MS 30m, 0.25mm ID, 0.25µm (cat.# 12723)Sample: 1µL. standards (component concentrations): cat.# 31812 (10µg/mL),

cat.# 31813 (50µg/mL), cat.# 31026 (50µg/mL), cat.# 31206 (20µg/mL). 10ppm each component on-column, except as noted.

Inj.: splitless (hold 0.4 min.), Drilled Uniliner® (cat.# 20756)Inj. temp.: 275°CCarrier gas: helium, constant flow Flow rate: 1mL/min. Oven temp.: 35°C (hold 1 min.) to 260°C @ 20°C/min. to 330°C @ 6°C/min. (hold 1 min.)Det: MSTransfer line temp.: 280°CScan range: 35–550amuIonization: EIMode: scan

1. benzaldehyde2. phenol3. bis(2-chloroethyl)ether4. 2-chlorophenol5. 1,2-dichlorobenzene-d4 (IS)*6. 2-methylphenol7. bis(2-chloroisopropyl)ether

8a. 4-methylphenol8b. 3-methylphenol

9. acetophenone10. N-nitroso-di-n-propylamine11. hexachloroethane12. nitrobenzene13. isophorone14. 2-nitrophenol15. 2,4-dimethylphenol16. bis(2-chloroethoxy)methane17. 2,4-dichlorophenol18. naphthalene-d8 (IS)*19. naphthalene20. 4-chloroaniline21. hexachlorobutadiene22 caprolactam23. 4-chloro-3-methylphenol24. 2-methylnaphthalene

25. hexachlorocyclopentadiene26. 1,2,4,5-tetrachlorobenzene27. 2,4,6-trichlorophenol28. 2,4,5-trichlorophenol**29. biphenyl30. 2-chloronaphthalene31. 2-nitroaniline**32. dimethylphthalate33. 2,6-dinitrotoluene34. acenaphthylene35. 3-nitroaniline**36. acenaphthene-d10 (IS)37. acenaphthene38. 2,4-dinitrophenol**39. 4-nitrophenol**40. dibenzofuran41. 2,4-dinitrotoluene42. diethyl phthalate43. fluorene44. 4-chlorophenyl phenyl ether45. 4-nitroaniline**46. 4,6-dinitro-2-methylphenol**47. diphenylamine48. 4-bromophenyl phenyl ether49. hexachlorobenzene

50. atrazine51. pentachlorophenol52. phenanthrene-d10 (IS)*53. phenanthrene54. anthracene55. di-n-butylphthalate56. fluoranthene57. pyrene58. butyl benzyl phthalate59. benzo(a)anthracene60. 3,3'-dichlorobenzidine**61. chrysene-d12 (IS)*62. chrysene63. bis(2-ethylhexyl)phthalate64. di-n-octyl phthalate65. benzo(b)fluoranthene66. benzo(k)fluoranthene67. benzo(a)pyrene68. perylene-d12 (IS)*69. indeno(1,2,3-cd)pyrene70. dibenz(a,h)anthracene71. benzo(ghi)perylene

GC_EV00683

*20ppm**50ppm

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Semivolatile OrganicsUS EPA Method 525.2Rtx®-5Sil MS

Rtx®-5Sil MS 30m, 0.25mm ID, 0.25µm (cat.# 12723)Sample: US EPA Method 525.2 Mix

1µL 10ppm (20ppm IS) Standards used: 31824, 32420, 32421, 32422, 32423, 31825, 31826, 31828, 32291

Inj.: 1.0µL 30psi 0.4min pulsed splitless (hold 0.4 min.), 4mm Drilled Uniliner® inlet liner (cat.# 21055)

Inj. temp.: 300°CCarrier gas: helium, constant flow Flow rate: 0.8 mL/min. Oven temp.: 35°C (hold 1 min.) to 270°C @

18°C/min. (hold 0 min.) to 330°C @ 5°C/min. (hold 1 min.)

Det: Agilent 5973 GC/MSTransfer line temp.: 280°CScan range: 40–550 amuSolvent Delay 4.0 min.Tune DFTPPIonization: EI

1. isophorone2. 2-nitro-m-xylene3. dichlorvos4. hexachlorocyclopentadiene5. EPTC6. mevinphos7. butylate8. vernolate9. dimethylphthalate

10. etridiazole (Terrazole®)11. 2,6-dinitrotoluene12. pebulate13. acenaphthylene14. acenaphthylene-d1015. chloroneb16. 2-chlorobiphenyl (BZ#1) 17. tebuthiuron18. 2,4-dinitrotoluene19. molinate20. diethyl phthalate21. fluorene22. propachlor23. ethoprop24. cycloate25. trifluralin 26. chlorpropham

27. α-BHC28. 2,3-dichlorobiphenyl (BZ#5)29. hexachlorobenzene30. atraton31. prometon32. simazine33. atrazine34. propazine35. β-BHC36. pentachlorophenol37. γ-BHC (lindane)38. terbufos39. diazinon40. pronamide41. phenanthrene-d1042. chlorothalonil43. phenanthrene44. methyl parathion OA45. disulfoton46. terbacil47. anthracene48. δ-BHC49. 2,4,5-trichlorobipenyl

(BZ#29)50. metribuzin51. alachlor

52. simetryn53. ametryn54. prometryne55. heptachlor 56. terbutryn57. di-n-butylphthalate58. bromacil59. 2,2',4,4'-tetrachloro-

biphenyl (BZ#47)60. metolachlor61. chlorpyrifos62. cyanazine (Bladex)63. DCPA methyl ester

(Dacthal®)64. aldrin65. triadimefon66. diphenamid67. MGK-26468. merphos69. heptachlor epoxide (isomer B)70. 2,2',3',4,6-pentachloro-

biphenyl (BZ#98)71. heptachlor epoxide (isomer A)72. stirofos (tetrachlorvinphos)73. butachlor74. α-chlordane

75. fenamiphos76. pyrene-d1077. γ-chlordane78. endosulfan I79. pyrene80. trans-nonachlor81. napropamide82. 4,4'-DDE83. tricyclazole84. p-terphenyl-d1485. 2,2',4,4',5,6'-hexachloro-

biphenyl (BZ#154)86. carboxin87. dieldrin 88. chlorbenzilate89. endrin 90. 4,4'-DDD91. endosulfan II92. endrin aldehyde 93. norflurazon94. butyl benzyl phthalate95. 4,4'-DDT96. bis(2-ethylhexyl)adipate97. endosulfan sulfate98. hexazinone99. triphenylphosphate

100. endrin ketone 101. 2,2',3,3',4,4',6-hepta-

chlorobiphenyl (BZ#171)102. methoxychlor 103. 2,2',3,3',4,5',6,6'-octa-

chlorobiphenyl (BZ#207)104. benzo(a)anthracene105. chrysene-d12106. chrysene107. bis(2-ethylhexyl)phthalate108. fenarimol109. cis-permethrin110. trans-permethrin111. benzo(b)fluoranthene112. benzo(k)fluoranthene113. fluridone (Sonar®)114. benzo(a)pyrene115. perylene-d12116. dibenzo(a,h)anthracene117. indeno(1,2,3-cd)pyrene118. benzo(ghi)perylene

GC_EV00699

GC_EV00698

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Semivolatiles564



Rtx®-5Sil MS 30m, 0.25mm ID, 0.25µm (cat.# 12723)Sample: US EPA Method 625 Mix 1µL 10ppm (20ppm IS)

Standards used: 31048, 31044, 31039, 31047, 31046, 31040, 31829Inj.: 1.0µL 20psi 0.3 min. pulsed splitless (hold 0.3 min.), 4mm Drilled Uniliner® inlet liner (cat.# 21055) Inj. temp.: 300°CCarrier gas: helium, constant flow Flow rate: 1.0mL/min. Oven temp.: 35°C (hold 1 min.) to 270°C @ 18°C/min.(hold 0 min.) to 305°C @ 5°C/min. (hold 0 min.)

To 330° @ 30°C (hold 1 min.) Det: Agilent 5973 GC/MSTransfer line temp.: 280°CScan range: 35-450 amuSolvent Delay 3.0 min.Tune DFTPPIonization: EI

1. N-nitrosodimethylamine2. pyridine-d5 (SS)3. 2-fluorophenol (SS)4. pentafluorophenol (IS)5. phenol6. bis(2-chloroethyl)ether7. 2-chlorophenol8. 1,3-dichlorobenzene9. 1,4-dichlorobenzene

10. 1,2-dichlorobenzene11. bis(2-chloroisopropyl)ether12. N-nitroso-di-n-propylamine13. hexachloroethane14. nitrobenzene-d5 (IS)15. nitrobenzene 16. isophorone17. 2-nitrophenol18. 2,4-dimethylphenol19. bis(2-chloroethoxy)methane20. 2,4-dichlorophenol21. 1,2,4-trichlorobenzene

22. naphthalene23. hexachlorobutadiene24. 4-chloro-3-methylphenol25. hexachlorocyclopentadiene26. 2,4,6-trichlorophenol27. 2-chloronaphthalene28. dimethylphthalate29. 2,6-dinitrotoluene30. acenaphthylene31. acenaphthene32. 2,4-dinitrophenol33. 4-nitrophenol34. 2,4-dinitrotoluene35. diethyl phthalate36. fluorene37. 4-chlorophenyl phenyl ether38. 4,6-dinitro-2-methylphenol39. diphenylamine40. 4,4'-dibromooctafluorophenol (SS)41. 4-bromophenyl phenyl ether42. hexachlorobenzene

43. pentachlorophenol44. phenanthrene45. anthracene46. di-n-butylphthalate47. 4,4'-dibromobiphenyl (IS)48. fluoranthene49. pyrene50. butyl benzyl phthalate51. benzo(a)anthracene52. chrysene53. bis(2-ethylhexyl)phthalate54. di-n-octyl phthalate55. benzo(b)fluoranthene56. benzo(k)fluoranthene57. benzo(a)pyrene58. indeno(1,2,3,-cd)pyrene59. dibenzo(a,h)anthracene60. benzo(ghi)perylene

GC_EV00700

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LSemivolatiles 565


Semivolatile OrganicsUS EPA Skinner ListRtx®-5Sil MS

GC_EV00684

Rtx®-5Sil MS 30m, 0.25mm ID, 0.25µm (cat.# 12723)Sample: Skinner SV MegaMix™ (cat.# 31690)

B/N Surrogate Mix (cat.# 31024)Acid Surrogate Mix (cat.# 31025)SV Internal Standard Mix (cat.# 31206)

Inj.: 1.0µL splitless (hold 0.4 min.), Drilled Uniliner® (cat.# 20756)Inj. temp.: 275°CCarrier gas: helium, constant flow Flow rate: 1mL/min. Oven temp.: 35°C (hold 1 min.) to 260°C @ 20°C/min. to 300°C @ 6°C/min. (hold 1 min.)Det: MSTransfer line temp.: 280°CScan range: 35-550amuIonization: EIMode: scan

1. pyridine2. 2-fluorophenol3. phenol-d6 (SS)4. phenol5. 1,3-dichlorobenzene6. 1,4-dichlorobenzene-d4 (IS)7. 1,4-dichlorobenzene8. 1,2-dichlorobenzene9. 2-methylphenol

10. indene11. 4-methylphenol12. 3-methylphenol

13. nitrobenzene-d5 (SS)14. 2,4-dimethylphenol15. naphthalene-d8 (IS)16. naphthalene17. quinoline18. 1-methylnaphthalene19. 2-fluorobiphenyl (SS)20. dimethylphthalate21. acenaphthene-d10 (IS)22. acenaphthene23. 2,4-dinitrophenol24. 4-nitrophenol

25. diethyl phthalate26. fluorene27. 2,4,6-tribromophenol (SS)28. phenanthrene-d10 (IS)29. phenanthrene30. anthracene31. di-n-butylphthalate32. fluoranthene33. pyrene34. p-terphenyl-d14 (SS)35. benzo(a)anthracene36. chrysene-d12 (IS)

37. chrysene38. bis(2-ethylhexyl)phthalate39. benzo(b)fluoranthene40. benzo(k)fluoranthene41. benzo(a)pyrene42. perylene-d12 (IS)43. dibenzo(a,h)acridine44. indeno(1,2,3-cd)pyrene45. dibenzo(a,h)anthracene

ScreeningMethod

a plus 1 story“Restek offers a wide range of columns and accessories for the environmental analyses performed in my lab. I am satisfied with the Restek products I have been using for several years-particularly the Rtx-5Sil MS and Rtx-CLPesticides columns and the deactivated inlet sleeves. The quality of the technical support provided byRestek is a plus.”

MMiicchheell LLeettoorrtt,, Veolia Environnement (Paris, France)

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Pesticides566


EDB / DBCPUS EPA Method 504.1Rtx®-CLPesticides & Rtx®-CLPesticides2

1. chloroform2. bromodichloromethane3. chlorodibromomethane4. 1,2-dibromoethane (EDB)5. 1,1,1,2-tetrachloroethane6. bromoform7. 1,2,3-trichloropropane8. 1,2-dibromo-3-chloropropane (DBCP)

30m, 0.32mm ID, 0.50µm Rtx®-CLPesticides (cat.# 11139) and30m, 0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324), 0.32mm ID guard column (cat.# 10044), universal angled“Y” Press-Tight® connector (cat.# 20403). Direct injection using a Uniliner® liner (cat.#20335). On-column conc.: 10pg each compound. Oven temp.: 35°C (hold 2 min.) to 300°C @ 12°C/min. Inj./det. temp.: 200°C/300°C Carrier gas: helium, 12psi constant pressure.

Rtx®-CLPesticides

Rtx®-CLPesticides2

min. 3 4 5 6 7 8 9 10

1

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min. 3 4 5 6 7 8 9 10

GC_EV00020

GGCC AAnnaallyyssiiss ooff UUSS EEPPAA MMeetthhoodd 550044..11OOrrggaannoocchhlloorriinnee PPeessttiicciiddeess,, UUssiinngg tthhee RRttxx®®--CCLLPPeessttiicciiddeess aanndd RRttxx®®--CCLLPPeessttiicciiddeess22CCoolluummnnss ((lliitt.. ccaatt..## 5599553399))A versatile column pair for analyzing organochlo-rine pesticides, herbicides, or PCBs

Analysts following Method 504.1 in monitoring1,2-dibromoethane (EDB), 1,2-dibromo-3-chloro-propane (DBCP), and 1,2,3-trichloropropane (TCP)in drinking water will value Rtx®-CLPesticides andRtx®-CLPesticides2 columns because this sameprimary column/confirmation column pair can beused to perform numerous related analyses:organochlorine pesticides (e.g., by EPA Method608 or 8081), herbicides, or polychlorinatedbiphenyls (PCBs). Details in this 2-page noteshow EDB, DBCP, and TCP are fully resolved fromcommon interference compounds, per require-ments of Method 504.1.

RRttxx®®--CCLLPPeessttiicciiddeess aanndd RRttxx®®--CCLLPPeessttiicciiddeess22ccoolluummnnss aallssoo aarree iiddeeaall ffoorr::

•• TTrriiaazziinnee hheerrbbiicciiddeess ((lliitt.. ccaatt..## 5599110011))•• PPCCBBss ((lliitt.. ccaatt..## 5599112200))•• HHaallooaacceettiicc aacciiddss ((lliitt.. ccaatt..## 5599117755))•• PPoollyynnuucclleeaarr aarroommaattiicc hhyyddrrooccaarrbboonnss

((lliitt.. ccaatt..## 5599119966AA))

• Dual-column analysis

free literature

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LPesticides 567


Organochlorine PesticidesUS EPA Method 508Rtx®-CLPesticides & Rtx®-CLPesticides2

min. 6 8 10 12 14 16 18 20

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Rtx®-CLPesticides2

1. hexachloropentadiene2. etridiazole3. chlorneb4. hexachlorobenzene5. propachlor6. α-BHC7. simazine8. γ-BHC9. atrazine

10. trifluralin11. pentachloronitrobenzene12. β-BHC13. δ-BHC14. metribuzin15. heptachlor16. aldrin17. chlorothalonil18. 4,4'-dibromobiphenyl19. alachlor20. metalachlor

21. DCPA (Dacthal®)22. heptachlor epoxide23. γ-chlordane24. cyanazine25. α-chlordane26. 4,4'-DDE27. endosulfan I28. dieldrin29. endrin30. chlorobenzilate31. 4,4'-DDD32. endosulfan II33. 4,4'-DDT34. endrin aldehyde35. methoxychlor36. endosulfan sulfate37. cis-permethrin38. trans-permethrin

30m, 0.32mm ID, 0.50µm Rtx®-CLPesticides (cat.# 11139)30m, 0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324)EPA 508.1 list w/ added compounds.

Oven temp.: 120°C (hold 1 min.) to 300°C @ 9°C/min. (hold 10 min.)Inj.: direct, Uniliner® inlet liner, 200°CDead time: 1.797 (Rtx®-CLPesticides), 1.663 (Rtx®-CLPesticides2) Head pressure: 12 psi (constant)Carrier gas: helium Flow: 2.1mL/min. @ 120°C

min. 6 8 10 12 14 16 18 20GC_EV00082

Rtx®-CLPesticides

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Organochlorine PesticidesUS EPA Method 508 Rtx®-5

min. 4 8 12 16 20 24 28 32 36 40 44 48 52

2 3

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2925

3130

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)2.0µL splitless injection

Splitless hold: 0.75 min.Oven temp.: 60°C to 300°C @ 4°C/min. (hold 10 min.)Inj. / det. temp.: 250°C / 320°CCarrier gas: heliumLinear velocity: 30cm/sec. (flow rate: 1.4cc/min.)

PPeeaakk CCoonncc..((ppgg//µLL))

1. etridiazole 502. chlorneb 10003. propachlor 10004. trifluralin 505. α-BHC 206. hexachlorobenzene 107. β-BHC 208. γ-BHC 309. PCNB (impurity) 100

10. PCNB (IS) 10011. δ-BHC 2012. chlorothalonil 5013. heptachlor 2014. aldrin 3015. DCPA 5016. DCB (SS) 500017. heptachlor epoxide 3018. γ-chlordane 3019. endosulfan I 3020. α-chlordane 3021. dieldrin 4022. endrin 3023. endosulfan II 3024. chlorobenzilate 100025. 4,4'-DDD 5026. endrin aldehyde 5027. endosulfan sulfate 3028. 4,4'-DDT 12029. methoxychlor 10030. cis-permethrin 100031. trans-permethrin 1000

1

GC_EV00083

three simple words...PPlluuss OOnnee service exceeding your expectations in everything we do

IInnnnoovvaattiivvee products and processes

EExxeeccuuttiioonn in our commitment to deliver a product, technical support, or other needs

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Organochlorine PesticidesUS EPA Method 8081 Rtx®-CLPesticides2

Rtx®-CLPesticides2 30m, 0.32mm ID, 0.25µm (cat.# 11324), intermediate-polaritydeactivated guard column, 5m x 0.32mm ID (cat.# 10044)Sample: Organochlorine Pesticide Mix AB #2 (cat.# 32292),

2,4,5,6-tetrachloro-m-xylene (ss) (cat.# 32027), decachlorobiphenyl (ss) (cat.# 32029), diluted in hexane, on-column amounts listed on figure

Inj.: 1.0µL splitless (hold 0.75 min.), 4mm Drilled Uniliner® inlet liner (cat.# 21055)

Inj. temp.: 225°CCarrier gas: hydrogen, constant pressureLinear velocity: 77cm/sec. @ 140°COven temp.: 140°C (hold 0.5 min.) to 268°C @ 30°C/min., to 290°C @

11°C/min., to 330°C @ 25°C/min. (hold 5 min.)Det.: ECD @ 320°C

Compound Conc. (ppb) RT (min.)1. 2,4,5,6-tetrachloro-m-xylene (ss) 80 2.4152. α-BHC 80 2.9163. γ-BHC 80 3.1924. β-BHC 80 3.2605. δ-BHC 80 3.4766. heptachlor 80 3.5187. aldrin 80 3.7498. heptachlor epoxide 80 4.1499. γ-chlordane 80 4.277

10. α-chlordane 80 4.37611. endosulfan I 80 4.41212. 4,4'-DDE 160 4.48913. dieldrin 160 4.59414. endrin 160 4.79415. 4,4'-DDD 160 4.86616. endosulfan II 160 4.93417. endrin aldehyde 160 5.16718. 4,4'-DDT 160 5.07819. endosulfan sulfate 160 5.36820. methoxychlor 800 5.58221. endrin ketone 160 5.79022. decachlorobiphenyl (ss) 160 6.940

GC_EV00734

Organochlorine PesticidesUS EPA Method 8081Rtx®-440 Rtx®-440 30m, 0.32mm ID, 0.50µm (cat.# 12939), intermediate-polarity deactivated guard

column, 5m x 0.32mm ID (cat.# 10044)Sample: Organochlorine Pesticide Mix AB #2 (cat.# 32292), 2,4,5,6-tetrachloro-

m-xylene (ss) (cat.# 32027), decachlorobiphenyl (ss) (cat.# 32029), diluted in hexane, on-column amounts listed on figure

Inj.: 1.0µL splitless (hold 0.75 min.), 4mm Drilled Uniliner® inlet liner (cat.# 21055)Inj. temp.: 225°CCarrier gas: hydrogen, constant pressureLinear velocity: 73cm/sec. @ 140°COven temp.: 140°C (hold 0.5 min.) to 268°C @ 30°C/min., to 290°C @ 11°C/min., to

330°C @ 25°C/min. (hold 5 min.)Det.: ECD @ 320°C

Compound Conc. (ppb) RT (min.)1. 2,4,5,6-tetrachloro-m-xylene (ss) 80 3.5382. α-BHC 80 3.9313. γ-BHC 80 4.2114. β-BHC 80 4.3565. δ-BHC 80 4.5606. heptachlor 80 4.7157. aldrin 80 4.9958. heptachlor epoxide 80 5.3469. γ-chlordane 80 5.576

10. α-chlordane 80 5.65311. endosulfan I 80 5.69812. 4,4’-DDE 160 5.80013. dieldrin 160 5.95914. endrin 160 6.19015. 4,4’-DDD 160 6.30916. endosulfan II 160 6.40417. endrin aldehyde 160 6.54318. 4,4’-DDT 160 6.66219. endosulfan sulfate 160 6.81020. methoxychlor 800 7.19821. endrin ketone 160 7.35522. decachlorobiphenyl (ss) 160 8.813

GC_EV00733

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1. 2,4,5,6-tetrachloro-m-xylene2. α-BHC (α-HCH)3. γ-BHC (lindane)4. β-BHC (β-HCH)5. δ-BHC (δ-HCH)6. heptachlor7. aldrin8. heptachlor epoxide9. γ-chlordane

10. α-chlordane11. 4,4'-DDE12. endosulfan I13. dieldrin14. endrin15. 4,4'-DDD16. endosulfan II17. 4,4'-DDT18. endrin aldehyde19. methoxychlor20. endosulfan sulfate21. endrin ketone22. decachlorobiphenyl

On-columnconcentration: 16–160pg organochlorine pesticide Mix AB#2 (cat.# 32292)Oven temp.: 120°C (hold 1 min.) to 300°C (hold 10 min.) @ 9°C/min.Inj. port: direct, Uniliner® liner (cat.# 20335), 200°CDetector: ECD, 300°C with anode purgeDead time: 1.9 min.Head pressure: 3psi (constant)Flow rate: 2.83mL/min. @ 120°C, helium

Rtx®-CLPesticides230m, 0.53mm ID, 0.42µm (cat.# 11340)


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Organochlorine PesticidesUS EPA Method 8081Rtx®-CLPesticides & Rtx®-CLPesticides2 (0.53mm ID)

min. 10 12 14 16 18 20 22

GC_EV00547

min. 10 12 14 16 18 20 22 24

On-column concentration: 16–160pg Organochlorine Pesticide Mix AB#2 (cat.# 32292), Pesticide

Surrogate Mix (cat. # 32000)Oven temp.: 120°C (hold 1 min.) to 300°C (hold 10 min.) @ 9°C/min.Inj.: direct, Uniliner® liner (cat.# 20335), 200°CDetector: ECD, 300°C with anode purge Dead time: 1.9 min.Head pressure: 8.7psi (constant)Flow rate: 1.3mL/min. @ 120°C, helium



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Organochlorine PesticidesUS EPA Method 8081Rtx®-CLPesticides & Rtx®-CLPesticides2 (0.32mm ID)

GC_EV00075min. 10 12 14 16 18 20 22 min. 10 12 14 16 18 20 22 24

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GC_EV00635

1. 2,4,5,6-tetrachloro-m-xylene2. α-BHC3. γ-BHC4. β-BHC5. δ-BHC6. heptachlor7. aldrin8. heptachlor epoxide9. γ-chlordane

10. α-chlordane11. 4,4'-DDE

12. endosulfan I13. dieldrin14. endrin15. 4,4'-DDD16. endosulfan II17. 4,4'-DDT18. endrin aldehyde19. methoxychlor20. endosulfan sulfate21. endrin ketone22. decachlorobiphenyl

Organochlorine PesticidesUS EPA Method 8081Rtx®-CLPesticides

GC_EV00636

Rtx®-CLPesticides 30m, 0.32mm ID, 0.50µm (cat.# 11139)Rtx®-CLPesticides2 30m, 0.32mm ID, 0.25µm (cat.# 11324)Sample: 20–400µg/mL Organochlorine Pesticide Mix AB #2 (cat.# 32292)

in hexane:toluene (1:1)200µg/mL Pesticide Surrogate Mix (cat.#32000 ) in acetone

Inj.: 1.0µL direct, open-top Drilled Uniliner® inlet liner (cat.# 21054) Inj. temp.: 225°CCarrier gas: helium, constant pressure Linear velocity: 53cm/sec. @ 110°COven temp.: 110°C (hold 1 min.) to 245°C @ 20°C/min. to 310°C (hold 1 min.)Det.: ECD @ 330°C

Rtx®-CLPesticides2 w/Drilled Uniliner® inlet liner

Rtx®-CLPesticides w/Drilled Uniliner® inlet liner

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GC_EV00512

1. 2,4,5,6 tetrachloro-m-xylene (SS)2. α-BHC3. γ-BHC4. β-BHC5. δ-BHC6. heptachlor7. aldrin8. heptachlor epoxide9. γ-chlordane

10. α-chlordane11. 4,4'-DDE12. endosulfan I13. dieldrin14. endrin15. 4,4'-DDD16. endosulfan II17. 4,4'-DDT18. endrin aldehyde19. methoxychlor20. endosulfan sulfate21. endrin ketone22. decachlorobiphenyl (SS)

Inj.: 1µL direct injection of 20/40/200ng/mL std. concentration in hexaneOven temp.: 110°C (hold 1 min.) to 245°C @ 20°C/min. to 300°C @ 6°C/min.Inj. & det. temp.: 210°C / 310°CCarrier gas: heliumDead time: 0.8min. @ 120°CInlet liner: Siltek™-deactivated Drilled Uniliner® inlet liner (cat.# 21055-214.5)Make-up gas: nitrogen

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Stx™-CLPesticides30m, 0.32mm ID, 0.5µm(cat.# 11544)

Stx™-CLPesticides230m, 0.32mm ID, 0.25µm(cat.# 11444)

A Siltek™-deactivated inlet linerimproves endrin response, rela-tive to conventionally deactivated liners.

Organochlorine PesticidesUS EPA Method 8081Stx™-CLPesticides & Stx™-CLPesticides2

min.

SSttxx™™--CCLLPPeessttiicciiddeess aanndd SSttxx™™--CCLLPPeessttiicciiddeess22CCoolluummnnss PPrroovviiddee IImmpprroovveedd SSyysstteemm IInneerrttnneessssffoorr CChhlloorriinnaatteedd PPeessttiicciiddeess AAnnaallyyssiiss

((lliitt.. ccaatt..## 5599335511BB))

An inert pathway assures maximum recovery ofactive chlorinated pesticides

Concern about losses of endrin and other activechlorinated pesticides generally is focused onthe injection port, but a properly deactivatedinjection system and an inert analytical column,in combination, are needed to minimize break-down. Inert Siltek™-treated inlet liners, guardcolumns and Stx™-CLPesticides and Stx™-CLPesticides2 analytical columns meet thisrequirement. This 2-page note also describesdeactivated connectors and chromatographicconditions for operating the primary and confir-mation column in parallel.

did you know?

for more info

566-591_GCAPP.qxd 11/4/2004 3:33 PM Page 572

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Organochlorine PesticidesUS EPA Method 8081A Rtx®-CLPesticides & Rtx®-CLPesticides2

Rtx®-CLPesticides2

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1. dibromochloropropane2. hexachlorocyclopentadiene3. 2,4,5,6-tetrachloro-m-xylene4. hexachlorobenzene5. cis-diallate6. α-BHC7. trans-diallate8. γ-BHC9. β-BHC

10. δ-BHC11. heptachlor12. aldrin13. isodrin14. heptachlor epoxide15. γ-chlordane16. α-chlordane17. 4,4'-DDE18. endosulfan I19. dieldrin20. chlorobenzilate21. endrin22. 4,4'-DDD23. endosulfan II24. 4,4'-DDT25. endrin aldehyde26. methoxychlor27. endosulfan sulfate28. endrin ketone29. decachlorobiphenyl

30m, 0.32mm ID, 0.50µm Rtx®-CLPesticides (cat.# 11139)30m, 0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324) On-column conc.: 16–160pgOven temp.: 80°C (hold 1 min.) to 300°C

@ 10°C/min. (hold 15 min.) Inj.: direct, Uniliner® inlet liner (cat.# 20335)Det.: ECD, 300°C, with anode purgeDead time: 1.9 min.Head pressure: 8.7psi (constant)Flow rate: helium @ 1.3mL/min. @ 120°C

Rtx®-CLPesticides

min. 10 15 20 25

min. 10 15 20 25

GC_EV00077

GGCC AAnnaallyyssiiss ooff UUSS EEPPAA MMeetthhoodd 550044..11OOrrggaannoocchhlloorriinnee PPeessttiicciiddeess,, UUssiinngg tthhee RRttxx®®--CCLLPPeessttiicciiddeess aanndd RRttxx®®--CCLLPPeessttiicciiddeess22CCoolluummnnss ((lliitt.. ccaatt..## 5599553399))

A versatile column pair for analyzingorganochlorine pesticides, herbicides, or PCBs

Analysts following Method 504.1 in monitoring1,2-dibromoethane (EDB), 1,2-dibromo-3-chloropropane (DBCP), and 1,2,3-trichloro-propane (TCP) in drinking water will value Rtx®-CLPesticides and Rtx®-CLPesticides2 columnsbecause this same primary column/confirma-tion column pair can be used to performnumerous related analyses: organochlorinepesticides (e.g., by EPA Method 608 or 8081),herbicides, or polychlorinated biphenyls (PCBs).Details in this 2-page note show EDB, DBCP,and TCP are fully resolved from common inter-ference compounds, per requirements ofMethod 504.1.

RRttxx®®--CCLLPPeessttiicciiddeess aanndd RRttxx®®--CCLLPPeessttiicciiddeess22ccoolluummnnss aallssoo aarree iiddeeaall ffoorr::

•• TTrriiaazziinnee hheerrbbiicciiddeess ((lliitt.. ccaatt..## 5599110011))•• PPCCBBss ((lliitt.. ccaatt..## 5599112200))•• HHaallooaacceettiicc aacciiddss ((lliitt.. ccaatt..## 5599117755))•• PPoollyynnuucclleeaarr aarroommaattiicc hhyyddrrooccaarrbboonnss

((lliitt.. ccaatt..## 5599119966AA))

free literature

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Organochlorine PesticidesUS EPA Method 8081Rtx®-XLB

1. 2,4,5,6-tetrachloro-m-xylene (ss)2. α-BHC3. γ-BHC4. β-BHC5. δ-BHC6. heptachlor7. aldrin8. heptachlor epoxide9. γ-chlordane

10. α-chlordane11. endosulfan I12. 4,4'-DDE13. dieldrin14. endrin15. 4,4'-DDD16. endosulfan II17. endrin aldehyde18. 4,4'-DDT19. endosulfan sulfate20. methoxychlor21. endrin ketone22. decachlorobiphenyl (ss)

GC_EV00720

Rtx®-XLB 30m, 0.32 ID, 0.5µm (cat.# 12839)Sample: Organochlorine Pesticide Mix AB (cat.# 32292) 80/160ppb in hexane

2,4,5,6-tetrachloro-m-xylene (cat.# 32027) surrogate, 80ppbdecachlorobiphenyl (cat.# 32029) surrogate, 160ppb

Inj.: 1.0µL splitless (0.75 min. hold), 4mm Drilled Uniliner® inlet liner (cat.# 21055)Inj. temp.: 220°CCarrier gas: hydrogen, constant pressureLinear velocity: 60cm/sec. @ 120°COven temp.: 120°C (hold 0.5 min.) to 260°C @ 29°C/min. (hold 2.5 min.), to 330°C @ 28°C/min. (hold 3 min.)Det.: ECD @ 320°C


www.restek.com

566-591_GCAPP.qxd 11/4/2004 3:33 PM Page 574

www.restek.com

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Organochlorine PesticidesUS EPA Method 8081ARtx®-XLB

1. 1,2-dibromo-3-chloropropane2. hexachlorocyclopentadiene3. 2,4,5,6-tetrachloro-m-xylene (ss)4. cis-diallate5. trans-diallate6. α-BHC7. hexachlorobenzene8. γ-BHC9. β-BHC

10. δ-BHC11. heptachlor12. aldrin13. isodrin14. heptachlor epoxide15. 2,4'-DDE16. γ-chlordane17. α-chlordane18. endosulfan I19. 4,4'-DDE20. 2,4'-DDD21. dieldrin22. chlorobenzilate23. endrin24. 2,4'-DDT25. 4,4'-DDD26. endosulfan II27. Kepone28. endrin aldehyde29. 4,4'-DDT30. endosulfan sulfate31. methoxychlor32. endrin ketone33. Mirex34. decachlorobiphenyl (ss)

GC_EV00721

Rtx®-XLB 30m, 0.32mm ID, 0.5µm (cat.# 12839)Sample: 8081A pesticides, 80-160ppb in hexaneInj.: 1.0µL splitless (hold 0.75 min.), 4mm Drilled Uniliner® inlet liner (cat.# 21055)Inj. temp.: 220°CCarrier gas: hydrogen, constant pressureLinear velocity: 60cm/sec. @ 120°COven temp.: 120°C (hold 0.5 min.) to 260°C @ 29°C/min. (hold 2.5 min.), to 330°C @ 28°C/min. (hold 3 min.)Det.: ECD @ 320°C

8081A Pesticides/Surrogates8080 Organochlorine Pesticide Mix AB #2 (20 components) cat.# 322928081a Organochlorine Pesticide Mix C #2 (7 components) cat.# 322952,4'-DDT cat.# 322002,4'-DDD cat.# 320982,4'-DDE cat.# 32099Kepone customMirex custom2,4,5,6-tetrachloro-m-xylene (ss, 20ppb) cat.# 32027decachlorobiphenyl (ss, 40ppb) cat.# 32029

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Organochlorine PesticidesUS EPA Method 8081Rtx®-35

min. 12 15 18 21 24

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221. 2,4,5,6-tetrachloro-m-xylene (surrogate)2. α-BHC3. γ-BHC (lindane)4. β-BHC5. δ-BHC6. heptachlor7. aldrin8. heptachlor epoxide9. γ-chlordane

10. α-chlordane11. 4,4'-DDE12. endosulfan I13. dieldrin14. endrin15. 4,4'-DDD16. endosulfan II17. 4,4'-DDT18. endrin aldehyde19. methoxychlor20. endosulfan sulfate21. endrin ketone22. decachlorobiphenyl (surrogate)

30m, 0.32mm ID, 0.50µm Rtx®-35 (cat.# 10439)Oven temp.: 120°C (hold 1 min.) to 285°C

@ 8.5°C/min. (hold 6 min.).Inj.: direct, Uniliner® inlet liner

(cat.# 20335), 200°C.Det.: ECD, 300°C with anode purgeFlow rate: helium @ 2.1mL/min. set @ 120°C

GC_EV00079

1. 2,4,5,6-tetrachloro-m-xylene (surrogate)2. α-BHC3. γ-BHC4. β-BHC5. δ-BHC6. heptachlor7. aldrin8. heptachlor epoxide9. γ-chlordane

10. α-chlordane11. 4,4'-DDE12. endosulfan I13. dieldrin14. endrin15. 4,4'-DDD16. endosulfan II17. 4,4'-DDT18. endrin aldehyde19. methoxychlor20. endosulfan sulfate21. endrin ketone22. decachlorobiphenyl (surrogate)

15m, 0.25mm ID, 0.25µm Rtx®-CLPesticides (cat.# 11120).2µL splitless injection. Conc.: 40 to 80ppb.Oven temp.: 125°C to 290°C @ 29°C/min. (hold 3 min.)Inj./det. temp.: 175°C/300°CCarrier gas: hydrogen @ 20psi constant pressure.

min. 1.0 2.0 3.0 4.0 5.0 6.0

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Organochlorine PesticidesUS EPA Method 8081Rtx®-CLPesticides

5

Courtesy of Bill DesJardins, ITS—Environmental, Colchester, Vermont

GC_EV00080

Fast Analysis!

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30m, 0.53mm ID, 0.50µm Rtx®-5 (cat.# 10240)1.0µL direct injection Conc.: 80–800ppb (ng/mL).

Oven temp.: 150°C (hold 5 min.) to 275°C @ 4°C/min. (hold 5 min.).

Inj. & det. temp.: 200°C / 275°C Carrier gas: heliumLinear velocity: 40cm/sec. set @ 150°C ECD sensitivity: 5.12 x 10-10 AFS

1. 2,4,5,6-tetrachloro-m-xylene (surrogate)

2. α-BHC3. γ-BHC4. heptachlor5. aldrin6. β-BHC7. δ-BHC8. heptachlor epoxide9. endosulfan I

10. γ-chlordane11. α-chlordane12. 4,4'-DDE13. dieldrin14. endrin15. 4,4'-DDD16. endosulfan II17. 4,4'-DDT18. endrin aldehyde19. endosulfan sulfate20. methoxychlor21. endrin ketone22. decachlorobiphenyl

(surrogate)

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GC_EV00408

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30m, 0.53mm ID, 0.25µm Rtx®-1701 (cat.# 12025)1.0µL direct injectionConc.: 80–800ppb (ng/mL).

Oven temp.: 150°C (hold 5 min.) to 275°C @4°C/min. (hold 5 min.).

Inj. & det. temp.: 200°C/275°C Carrier gas: heliumLinear velocity: 40cm/sec. set at 150°C.ECD sensitivity: 5.12 x 10-10 AFS

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GC_EV00409

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1. 2,4,5,6-tetrachloro-m-xylene (SS)2. α-BHC3. γ-BHC4. β-BHC5. heptachlor6. δ-BHC7. aldrin8. heptachlor epoxide9. γ-chlordane

10. α-chlordane11. endosulfan I12. 4,4'-DDE13. dieldrin14. endrin15. 4,4'-DDD16. endosulfan II17. 4,4'-DDT18. endrin aldehyde19. endosulfan sulfate20. methoxychlor21. endrin ketone22. decachlorobiphenyl

11

30m, 0.53mm ID, 0.83µm Rtx®-50 (cat.# 10569)1.0µL direct injectionConc.: 80–800ppb (ng/mL).

Oven temp.: 150°C (hold 5 min.) to 275°C @ 8°C/min.(hold 5 min.)

Inj. & det. temp.: 200°C / 275°CCarrier gas: heliumLinear velocity: 40cm/sec. set @ 150°CECD sensitivity: 5.12 x 10-10 AFS

min. 5 10 15 20 25

GC_EV00410

EPA Method 8081Chlorinated PesticidesRtx®-50

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Organophosphorus PesticidesUS EPA Method 8141ARtx®-OPPesticides2

GC_EV00602

1. dichlorvos2. hexamethylphosphoramide3. mevinphos4. trichlorfon5. TEPP6. demeton-O7. thionazin8. tributyl phosphate (IS)9. ethoprop

10. naled11. sulfotepp12. phorate13. dicrotophos14. demeton-S15. monocrotophos16. terbufos17. dimethoate18. dioxathion

19. fonophos20. diazinon21. disulfoton22. phosphamidon isomer 23. dichlorofenthion24. chlorpyrifos methyl25. phosphamidon26. parathion-methyl27. ronnel28. fenitrothion29. aspon30. malathion31. chlorpyrifos32. trichloronate33. parathion-ethyl34. fenthion35. merphos36. chlorfenvinphos

37. crotoxyphos38. stirofos39. tokuthion40. merphos oxone

(breakdown product) 41. ethion42. fensulfothion43 bolstar44. carbophenothion45. famphur46. triphenyl phosphate (SS)47. EPN48. phosmet49. leptophos50. tri-o-cresyl phosphate51. azinphos-methyl52. azinphos-ethyl 53. coumaphos

Column: Rtx®-OPPesticides2, 30m, 0.25mm ID, 0.25µm (cat.# 11243)Sample: US EPA Method 8141A Custom Standard Mix 1µL 100ppm

(100ng on column) Triphenylphosphate Standard (cat.# 32281)Tributylphosphate Standard (cat.# 32280)8140/8141 OP Pesticides Calibration Mix A (cat.# 32277)8141 OP Pesticides Calibration Mix B (cat.# 32278)

Custom Standard Mixes: Call Restek for More InformationInj.: 1.0µL splitless (hold 0.4 min.), 4mm double

gooseneck inlet liner (cat.# 20785)

Inj. temp.: 250°CCarrier gas: helium, constant flow Flow rate: 1.0mL/min. Oven temp.: 80°C (hold 0.5 min.) to 140°C @ 20°C/min.

to 210°C @ 4°C/min. (hold 1 min.) to 280°C @ 30°C (hold 5 min.)

Det: MSTransfer line temp.: 280°CScan range: 35-400amuIonization: EI

++ Pesticide Standards ==

Partners in Pesticide Analysis

For more information on the ChemService product line, please see page 377 or visit www.restek.com/csi.

for more infoGGCC CCoolluummnnss ffoorr PPeessttiicciiddee AAnnaallyysseessChoose from 9 capillary GC phases for pesticide analy-ses and more than 50 combinations of column ID andphase film thickness. For descriptions of special purposecolumns, sseeee ppaaggeess 2222--2233. To locate a column type inthis catalog, sseeee ppaaggee 1133.

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LPesticides 579


GC: splitless, purge on 1.0 min. constant pressureOven temp.: 80°C (hold 0.5 min.) to 280°C

@ 12°C/min. (hold 10 min.)Injector: 200°CInlet liner: 4mm single gooseneck Siltek™ inlet linerDetector: FPD @ 250°CDead time: 1.03 min. @ 80°CInjection: 1µL US EPA Method 8141A Custom

Standard Mixes (100ng/mL)Triphenylphosphate Standard (cat.# 32281)Tributylphosphate Standard (cat.# 32280)8140/8141 OP Pesticides Calibration Mix A (cat.# 32277)8141 OP Pesticides Calibration Mix B (cat.# 32278)

dual-column injector

Organophosphorus PesticidesUS EPA Method 8141ARtx®-OPPesticides & Rtx®-OPPesticides2

1. dichlorvos2. hexamethylphosphoramide3. mevinphos4. trichlorfon5. TEPP6. demeton-O7. tributyl phosphate (SS)8. thionazin9. ethoprop

10. naled11. sulfotepp12. phorate13. dicrotophos14. monocrotophos15. demeton-S16. terbufos17. dimethoate18. diazinon19. dioxathion20. fonophos21. disulfoton22. phosphamidon isomer

(breakdown product)23. dichlorofenthion24. phosphamidon25. chlorpyrifos methyl26. parathion-methyl27. ronnel28. aspon29. fenitrothion30. malathion31. chlorpyrifos32. trichloronate33. parathion-ethyl34. fenthion35. merphos36. chlorfenvinphos37. crotoxyphos38. stirofos39. tokuthion40. merphos oxone

(breakdown product)41. ethion42. fensulfothion43. bolstar44. carbophenothion45. famphur46. triphenyl phosphate (SS)47. EPN48. phosmet49. leptophos50. tri-o-cresyl phosphate51. azinphos-methyl52. azinphos-ethyl53. coumaphos

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

1718

19,20

21

22

23

24

25

26

27

28

29

31

32,33

5352

5150

49

4847

46

45

44

43

42

41

40

39

38

37

36

35

34

30

1

2 4

3

6

8

9

12

10,11

7

18

16

5,20

19,21

15

13,14

31

28

32

26,30

29

22

23

25

17

27,35

34

39

24

36,33

40

3837

43

44,41

46

4942

50

47,48

45

51

52

53

min. 8 10 12 14 16 18 20 22

min. 6 8 10 12 14 16 18 20

Rtx®-OPPesticides30m, 0.32mm ID, 0.50µm(cat.# 11239)

Rtx®-OPPesticides230m, 0.32mm ID, 0.32µm(cat.# 11241)

GC_EV00394

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Organophosphorus PesticidesUS EPA Method 8140/8141/8141ARtx®-1701

min. 2 4 6 8 10 12 14 16 18

1. dichlorvos2. hexamethylphosphoramide3. mevinphos4 trichlorfon5. demeton-O6. thionazin7. ethoprop8. tributyl phosphate (SS)9. TEPP

10. naled11. phorate

12. sulfotepp13. terbufos14. demeton-S15. diazinon16. fonophos17. dicrotophos18. dioxathion19. disulfoton20. dichlorofenthion21. monocrotophos22. chlorpyrifos methyl

23. dimethoate24. phosphamidon isomer25. ronnel26. merphos27. aspon28. chlorpyrifos29. trichloronate30. parathion-methyl31. phosphamidon32. fenthion33. malathion

34. fenitrothion35. parathion-ethyl36. chlorfenvinphos37. tokuthion38. merphos oxone39. crotoxyphos40. stirofos41. ethion42. bolstar43. carbophenothion44. fensulfothion

45. triphenyl phosphate (SS)46. famphur47. leptophos48. EPN49. tri-o-cresyl phosphate50. phosmet51. azinphos-methyl52. azinphos-ethyl53. coumaphos

30m, 0.32mm ID, 0.25µm Rtx®-1701 (cat.# 12024)1µL US EPA Method 8141A Custom Standard Mixes (200ng/mL)

Triphenylphosphate Standard (cat.# 32281)Tributylphosphate Standard (cat.# 32280)8140/8141 OP Pesticides Calibration Mix A (cat.# 32277)8141 OP Pesticides Calibration Mix B (cat.# 32278)

Splitless hold time: 1.0 min.Oven temp.: 100°C to 180°C @ 10°C/min. (hold 2 min.), to

300°C @ 18°C/min. (hold 3 min.)Inj. / det. temp.: 250°C / 280°C FPD

1

2

3

4

5

6

7,8 (SS)

9

10,11

12

13,14,15

16,17 18,19

2021

23,24

22

25

26

27,28

29,30

31

3233

34

35

3637

3839

40

41,42

43(SS)

44

45 4647

48

49

5051 52

53

GC_EV00411


Column: Rtx®-35 30m, 0.32mm ID, 0.25µm (cat.# 10424)Sample: 0.1µg/mL US EPA Method 8141A Custom Standard Mixes (100ng/mL)


Inj.: 0.5µL splitless (hold 5 min.), Siltek™ Drilled Uniliner® inlet liner(cat.# 21054-214.1)

Inj. temp.: 220°CCarrier gas: helium, constant pressure Linear velocity: 42cm/sec. @ 60°COven temp.: 100°C to 180°C @10°C/min. (hold 2 min.) to 300°C

@ 18°C/min. (hold 10 min.) Det.: FPD @ 280°C

1. dichlorvos2. hexamethylphosphoramide3. mevinphos4. trichlorfon5. tributyl phosphate (SS)6. demeton-O7. TEPP8. thionazin9. ethoprop

10. sulfotepp11. naled12. phorate13. dicrotophos14. demeton-S15. terbufos16. monocrotophos17. diazinon18. fonophos19. disulfoton

20. dioxathion21. dimethoate22. phosphamidon isomer23. dichlorofenthion24. chlorpyrifos methyl25. phosphamidon26. ronnel27. parathion-methyl28. aspon29. trichloronate30. chlorpyrifos31. fenitrothion32. merphos33. malathion34. parathion-ethyl35. fenthion36. chlorfenvinphos37. crotoxyphos38. merphos oxone

39. tokuthion40. stirofos41. ethion42. bolstar43. fensulfothion44. carbofenothion45. famphur46. triphenyl phosphate (SS)47. EPN48. phosmet49. leptophos50. tri-o-cresyl phosphate51. azinphos-methyl52. azinphos-ethyl53. coumaphos

GC_EV00696

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LPesticides 581


Organophosphorus PesticidesUS EPA Method 8140/8141/8141ARtx®-CLPesticides

30m, 0.32mm ID, 0.50µm Rtx®-CLPesticides (cat.# 11139)1µL US EPA Method 8141A Custom Standard Mixes (200ng/mL)


splitless hold time: 1 min.Oven temp.: 100°C to 180°C @ 10°C/min.

(hold 2 min.), to 300°C @18°C/min. (hold 3 min.)

Inj. / det. temp.: 250°C / 280°C FPD

min. 2 4 6 8 10 12 14 16 18

1

2

3

4

5

6

7

8

9

10 (SS), 11, 12

13,14,15

16,17,18

19,20,21 2223

24

25

26

27

28

29,30

3132

3334

35

36

37

38

39,40

41

42

43

44,45(SS)

46

4748

49

50

51 52

53

1. dichlorvos2. hexamethylphosphoramide3. trichlorfon4. mevinphos5. demeton-O6. thionazin7. ethoprop8. phorate9. naled

10. tributyl phosphate (SS)11. TEPP

12. sulfotepp13. demeton-S14. terbufos15. fonophos16. dicrotophos17. diazinon18. disulfoton19. dioxathion20. monocrotophos21. dimethoate22. dichlorofenthion

23. chlorpyrifos methyl24. ronnel25. phosphamidon isomer26. merphos27. chlorpyrifos28. fenthion29. aspon30. parathion-methyl31. trichloronate32. malathion33. fenitrothion

34. phosphamidon35. parathion-ethyl36. chlorfenvinphos37. tokuthion38. merphos oxone39. crotoxyphos40. stirofos41. bolstar42. famphur43. carbophenothion44. fensulfothion

45. triphenyl phosphate (SS)46. leptophos47. ethion48. phosmet49. EPN50. tri-o-cresyl phosphate51. azinphos-methyl52. azinphos-ethyl53. coumaphos

GC_EV00413

Organophosphorus PesticidesUS EPA Method 8140/8141/8141ARtx®-CLPesticides2

2 4 6 8 10 12 14 16 18 min.

1

2

3

4

5

6

7(SS)

8,9

10

11

12

13,14,15

16

17

1819

20,21

22

23

2425

26

27

2829,30

31

32,33,34

35

3637 38

39,40

41

42

43

44

45(SS),46

47

48,49

51 5253

1. dichlorvos2. hexamethylphosphoramide3. mevinphos4. trichlorfon5. demeton-O6. thionazin7. tributyl phosphate (SS)8. ethoprop9. TEPP

10. naled11. phorate

12. sulfotepp13. demeton-S14. dicrotophos15. dioxathion16. terbufos17. monocrotophos18. fonophos19. diazinon20. disulfoton21. dimethoate22. dichlorofenthion

23. phosphamidon isomer24. chlorpyrifos methyl25. ronnel26. parathion-methyl27. phosphamidon28. aspon29. chlorpyrifos30. trichloronate31. merphos32. fenitrothion33. fenthion

34. malathion35. parathion-ethyl36. chlorfenvinphos37. crotoxyphos38. tokuthion39. merphos oxone40. stirofos41. famphur42. bolstar43. carbophenothion44. fensulfothion

45. triphenyl phosphate (SS)46. ethion47. EPN48. phosmet49. leptophos50. tri-o-cresyl phosphate51. azinphos-methyl52. azinphos-ethyl53. coumaphos

30m, 0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324)1µL US EPA Method 8141A Custom Standard Mixes (200ng/mL)


splitless hold time: 1 min.Oven temp.: 100°C to 180°C @

10°C/min. (hold 2 min.), to 300°C @ 18°C/min. (hold 3 min.)

Inj. / det. temp.: 250°C / 280°C FPD

GC_EV00414

50

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30m, 0.32mm ID, 0.50µm Rtx®-1 (cat.# 10139)Total OPP custom standard mix (0.2µg/mL), 1µL US EPA Method 8141A Custom Standard Mixes (200ng/mL)


splitless hold time: 1 min.Oven temp.: 100°C to 180°C @ 10°C/min. (hold 2 min.), to 300°C @ 18°C/min. (hold 3 min.)Inj. / det. temp.: 250°C / 280°C FPDSolvent: hexaneDead time: 0.78min. @ 100°C, constant pressure

1. dichlorvos2. hexamethylphosphoramide3. mevinphos4. trichlorfon5. TEPP6. thionazin7. demeton-O8. ethoprop9. tributyl phosphate (SS)

10. dicrotophos11. monocrotophos12. naled13. sulfotepp14. phorate15. dimethoate16. demeton-S17. dioxathion18. terbufos19. fonophos20. phosphamidon isomer21. diazinon22. disulfoton23. phosphamidon24. dichlorofenthion25. parathion-methyl26. chlorpyrifos methyl27. ronnel28. fenitrothion29. malathion30. fenthion31. aspon32. parathion-ethyl33. chlorpyrifos34. trichloronate35. chlorfenvinphos36. merphos37. crotoxyphos38. stirofos39. tokuthion40. merphos oxidation product41. fensulfothion42. famphur43. ethion44. bolstar45. carbophenothion46. triphenyl phosphate (SS)47. phosmet48. EPN49. azinphos-methyl50. leptophos51. tri-o-cresyl phosphate52. azinphos-ethyl53. coumaphos

1

2

3

4

5

6(IS)

7

8

9,10

11,12

13

14

15

16,17

18,19

20

21

22

23

24,25

26

27

28

29

30

31,32,33

34

35

36,37

38

39

40

41

42

43,44

45

46(IS)47

48

4950

51

52 53

GC_EV00415min. 2 4 6 8 10 12 14 16

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LPesticides 583


GC_EV00676

Rtx®-CLPesticides 30m, 0.32mm ID, 0.50µm (cat.# 11139)Sample: European OPP Mix (cat.# 32418)

100–1000µg/mL in acetone Inj.: 0.5µL direct, open-top Siltek™ Drilled Uniliner®

inlet liner (cat.# 21055-214.5)Inj. temp.: 250°CCarrier gas: helium, constant pressure Linear velocity: 35cm/sec. @ 80°COven temp.: 80°C (hold 1 min.) to 150°C @ 7°C/min.

to 280°C @ 15°C/min. (hold 10 min.)Det.: FPD @ 280°C

CCoonncceennttrraattiioonnoonn--ccoolluummnn ((nngg))

1. methamidophos 1.252. dichlorvos 1.253. acephate 0.504. demeton-S-methyl 0.505. tributylphosphate (IS) 1.256. omethoate 2.507. dimethoate 0.508. tolclofos-methyl 0.259. pirimiphos methyl 0.25

10. chlorpyriphos 0.2511. malathion 0.5012. tokuthion 0.5013. methidathion 0.5014. profenfos 0.5015. ethion 0.5016. triphenylphosphate (surrog.) 1.2517. azinphos-methyl 1.0018. pyrazophos 1.25

Organophosphorus Pesticides (European)Rtx®-CLPesticides


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Organophosphorus Pesticides (European)Rtx®-CLPesticides

GC_EV00648

Rtx®-CLPesticides, 30m, 0.25mm ID, 0.25µm (cat.# 11123)Sample: Custom European Mix (call for details)

1-bromo-2-nitrobenzene cat.# 322794-chloro-3-nitrobenzotrifluoride cat.# 32282tributylphosphate cat.# 32280triphenylphosphate cat.# 32281

Inj.: 1.0µL splitless (hold 0.4 min.)4mm double gooseneck inlet liner (cat.# 20785)

Inj. temp.: 250°CCarrier gas: helium, constant flow, 6 psi head pressure Flow rate: 0.75mL/min.Linear velocity: 28cm/sec.* Dead time: 1.82 min. @ 80°C Oven temp.: 80°C (hold 1.0 min.) to 150°C @ 7°C/min. (no hold)

to 280°C @ 15°C/min. (hold 7 min.) Det.: Agilent 5971A GC/MSTransfer line temp.: 280°CScan range: 35–400 amuSolvent Delay 5 min.Tune PFTBAIonization: EI

CCoommppoouunndd CCoonncc.. oonn--ccoolluummnn ((nngg))1. 4-chloro-3-nitro-trifluoride 1002. methamidophos 503. dichlorvos 504. 1-bromo-2-nitrobenzene 1005. acephate 206. demeton-S-methyl 207. TBP (int. std.) 1008. omethoate 1009. dimethoate 20

10. tolclofos-methyl 1011. pirimiphos methyl 1012. chlorpyrifos 1013. malathion 2014. quinalphos 1015. tokuthion (prothiofos) 2016. methidathion 2017. profenfos 2018. ethion 2019. TPP (surrog.) 10020. azinphos-methyl 4021. pyrazophos 50*This mix was prepared for FPD analyses. Peaks will be equivalent height with

FPD detection if linear velocity is set at 28cm/sec. (0.32mm ID column).

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LPesticides/Herbicides 585


1

2

34

5

7

6

30m, 0.32mm ID, 0.50µm Rtx®-CLPesticides (cat.# 11139) and30m, 0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324)with a 5m, 0.32mm ID guard column (cat.# 10044) and a ‘Y’ Press-Tight® connector (cat.# 20403). Direct injection using a Uniliner® liner (cat.#21303) and adaptor for an HP 5890 (cat.# 20964). On-column concentration 50pg each compound.

Oven temp.: 100°C (hold 0 min.) to 250°C @ 4°C/min. (hold 5 min.); Inj./det. temp.: 250°C/275°CCarrier gas: hydrogen, 9.65psi constant pressureGC: HP 6890 with purged packed injection port.

Rtx®-CLPesticides

8

min. 18 19 20 21 22 23

9 10 11

CAS No.1. prometon 1610-18-0 2. simazine 122-34-93. atraton 1610-17-94. propazine 139-40-25. atrazine 1912-24-96. terbuthylazine 5915-41-37. secbumeton 26259-45-08. simetryne 1014-70-69. ametryne 834-12-8

10. prometryne 7287-19-611. terbutryne 886-50-0

Nitrogen/Phosphorus Pesticides & HerbicidesUS EPA Method 619Rtx®-CLPesticides & Rtx®-CLPesticides2

1

2

3

4

5

7

6

8

min. 18 19 20 21 22 23

9

10 11

Rtx®-CLPesticides2

GC_EV00056

Dual-columnanalysis

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Pesticides/Herbicides586


Nitrogen/Phosphorus Pesticides & HerbicidesRtx®-5

GC_EV00089

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)0.1µL splitless injection, concentration 1.5–29.9ppm

Splitless hold: 0.75 min.Oven temp.: 100°C (hold 5 min.) to 200°C @

15°C/min. (hold 8 min.), to 290°C @15°C/min. (hold 5 min.)

Inj. & det. temp.: 290°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 100°CNPD sensitivity: 8 x 10-11 AFS

1. 2-nitro-m-xylene2. molinate3. dimethoate4. atrazine5. Diazinon™

6. prometryne7. malathion8. DEF™

min. 5 10 15 20 25 30

18

7

6

5

4

3

2

Permission to publish this chromatogram granted by Canonie Environmental Services.

Nitrogen/Phosphorus Pesticides & HerbicidesRtx®-5

GC_EV00090

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)0.1µL splitless injection, concentration 1.5–29.9ppm.

Splitless hold: 0.75 min.Oven temp.: 100°C (hold 5 min.) to 200°C @

15°C/min. (hold 8 min.), to 290°C @ 15°C/min. (hold 5 min.)

Inj. & det. temp.: 290°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 100°CNPD sensitivity: 8 x 10-11 AFS

1. phorate2. demeton3. simazine4. disulfoton5. methyl parathion6. bromacil7. ethyl parathion8. ethion

min. 5 10 15 20 25 30

2

1

3

4

5

6

7

8

Permission to publish this chromatogram granted by Canonie Environmental Services.

566-591_GCAPP.qxd 11/4/2004 3:33 PM Page 586

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LHerbicides 587


Chlorophenoxyacid HerbicidesUS EPA Method 615Rtx®-35

min. 5 10 15 20 25 30

2

54

3

7

8

9 1012

11

61

1. dalapon2. DCAA (S)3. dicamba4. MCPP5. MCPA6. DBOB (IS)7. dichlorprop8. 2,4-D9. 2,4,5-TP

10. 2,4,5-T11. dinoseb12. 2,4-DB

GC_EV00094

Analysis optimizedusing Pro ezGC™

software!See page 449.

30m, 0.53mm ID, 0.50µm Rtx®-35 (cat.# 10440)0.5µL direct injection of chlorophenoxy herbicides,on-column concentration 10–10,000µg/mL

Oven temp.: 60°C to 150°C @ 8°C/min. (hold 5 min.), to 210°C @ 4°C/min.

Inj./det. temp.: 250°C / 275°CCarrier gas: heliumLinear velocity: 35cm/sec. set @ 60°CDet.: ECD w/anode purge

Chlorophenoxyacid HerbicidesUS EPA Method 615Rtx®-5

min. 5 10 15 20 25 30GC_EV00095

30m, 0.53mm ID, 0.50µm Rtx®-5 (cat.# 10240)0.5µL direct injection of chlorophenoxy herbicides,on-column concentration 10–10,000µg/mL

Oven temp.: 60°C to 150°C @ 8°C/min. (hold 5 min.),to 210°C @ 4°C/min.

Inj./det. temp.: 250°C / 275°CCarrier gas: heliumLinear velocity: 35cm/sec. set @ 60°CDet.: ECD w/anode purge

1 2

3

4

5

7

8 6

910

12

11

Analysis optimizedusing Pro ezGC™


1. dalapon2. DCAA (S)3. dicamba4. MCPP5. MCPA6. DBOB (IS)7. dichlorprop8. 2,4-D9. 2,4,5-TP

10. 2,4,5-T11. dinoseb12. 2,4-DB

for more infoon Pro ezGC™ software, seeppaaggee444499.

566-591_GCAPP.qxd 11/4/2004 3:33 PM Page 587

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Herbicides588


Triazine HerbicidesUS EPA Method 619Rtx®-50, Rtx®-200

min. 10 12 14 16

GC_EV00058

1

11

10

9

7

8

6

5

43

2

1. atraton2. prometon3. terbutylazine4. atrazine5. simazine6. propazine7. secbumeton8. terbutryne9. ametryne

10. simetryne11. prometryne

0.5µL direct injection of EPA Method 619 - triazine herbicides.On-column concentration: 50ng


Inj. / det. temp.: 250°C /275°CDetector: TSDCarrier gas: heliumLinear velocity: 40cm/sec. set @ 150°C

Rtx®-5030m, 0.53mm ID, 0.50µm (cat.# 10540)

Rtx®-200 30m, 0.53mm ID, 0.50µm (cat.# 15040)

10

9,11

8

6

54

3,7

2

1

GC_EV00059min. 4 6 8 10

a plus 1 story“Thank you for being a top notch GC Analytical Supply Company that always continues to offer newer products & services.”

JJoohhnn TT.. BBrruunneettttee,, Chemist, Bausch & Lomb

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LHerbicides 589


Chlorophenoxyacid HerbicidesUS EPA Method 8151Rtx®-CLPesticides &Rtx®-CLPesticides2

1

2

3

4

56

7

8

9

10

11

12

13

14,15

16

17

18

min. 4 6 8 10 12 14 16 18 20 22

30m, 0.32mm ID, 0.50µm, Rtx®-CLPesticides (cat.# 11139) and 30m, 0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324). 1.0µL direct injection of derivatized chlorophenoxy herbicides, Uniliner® inlet liner (cat.# 20335),on-column conc.: 200–20,000pg/mL

Oven temp.: 80°C (hold 1 min.) to 300°C @ 10°C/min. (hold 10 min.)Inj. / det. temp.: 200°C/300°CCarrier gas: heliumInlet pressure: 12.5psi set @ 80°CDet.: ECD w/anode purge

Rtx®-CLPesticides Rtx®-CLPesticides2

1. dalapon2. 3,5-dichlorobenzoic acid3. 4-nitroanisole4. dicamba5. MCPP6. MCPA7. dichlorprop8. 2,4-D9. pentachloroanisole

10. 2,4,5-TP11. chloramben 12. 2,4,5-T13. 2,4-DB14. picloram 15. bentazon16. dinoseb 17. DCPA18. acifluorfen

1

2

3

4

5

67

8

9

10

11

12

13

14

16

17

18

15

min. 5 10 15 20

DCAA

DCAA

GC_EV00091

Chlorophenoxyacid HerbicidesUS EPA Method 515.1Rtx®-50

30m, 0.25mm ID, 0.50µm Rtx®-50 (cat.# 10538)2.0µL splitless injection of derivatized chlorophenoxy herbicides. Conc. 10–187pg/µL

Oven temp.: 50°C (hold 0.75 min.) to 84°C @ 4°C/min., to 165°C @ 10°C/min., to 270°C @ 4°C/min., to 300°C @ 20°C/min. (hold 6 min.)

Inj. / det. temp.: 220°C / 320°CCarrier gas: helium, constant pressureLinear velocity: 30cm/sec. set @ 50°CSplitless hold time: 0.75 min.

min. 4 8 12 16 20 24 28 32 36 40 44 48

GC_EV00093

1

2

34

5

6

8

9

711

10

1. dalapon2. 2,4-dichlorophenylacetic acid3. dicamba4. dibromooctafluorobiphenyl5. 2,4-D6. pentachlorophenol7. Silvex (2,4,5-TP)8. 2,4,5-T9. dinoseb

10. 2,4-DB11. picloram

566-591_GCAPP.qxd 11/4/2004 3:33 PM Page 589

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Herbicides590


Nitrogen-Containing HerbicidesRtx®-5

GC_EV00087

1. Eptam®

2. Sutan®

3. Vernam®

4. Tillam®

5. Ordram®

6. propachlor7. Ro-Neet®

8. Treflan®

9. Balan®

10. simazine11. atrazine

12. propazine13. Tolban®

14. terbacil15. Sencor®

16. bromacil17. Dual®

18. Paarlan®

19. Prowl®

20. oxadiazon21. GOAL®

22. hexazinone

30m, 0.53mm ID, 0.50µm Rtx®-5 (cat.# 10240)0.2µL direct injection, Uniliner® inlet liner, concentration approximately 10ngper component.


Inj. & det. temp.: 290°CCarrier gas: heliumLinear velocity: 40cm/sec. (flow rate: 5.2cc/min.) FID sensitivity: 16 x 10-11 AFS

min. 2 4 6 8 10 12 14 16 18

22

2120

19

18

17

16

15

14

13

12

11

10

98

76

542

3

1

Nitrogen-Containing HerbicidesRtx®-35

GC_EV00088

min. 2 4 6 8 10 12 14 16 18 20

1 2 34 5

6

89

10 1112

1314

15

16

17

18,19

20 21 22

30m, 0.53mm ID, 0.50µm Rtx®-35 (cat.# 10440)0.2µL direct injection, Uniliner® liner, concentration approximately 10ng percomponent.


Inj. & det. temp.: 290°CCarrier gas: heliumLinear velocity: 40cm/sec. (flow rate: 5.2cc/min.) FID sensitivity: 16 x 10-11 AFS

1. Eptam®

2. Sutan®

3. Vernam®

4. Tillam®

5. Ordram®

6. Treflan®

7. Balan®

8. Ro-Neet®

9. propachlor10. Tolban®

11. propazine

12. atrazine13. simazine14. terbacil15. Sencor®

16. Dual®

17. Paarlan®

18. Prowl®

19. bromacil20. oxadiazon21. GOAL®

22. hexazinone7

566-591_GCAPP.qxd 11/4/2004 3:33 PM Page 590

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LHerbicides 591


Triazine Herbicides (French) & Degradation ProductsRtx®-OPPesticides2

GC_EV00626

PPeeaakk LLiisstt:: CCAASS##1. trifluraline 1582-09-82. desisopropylatrazine 1007-28-93. desethyl-atrazine 6190-65-4 4. desethyl-terbuthylazine —-5. simazine 122-34-96. atrazine 1912-24-97. terbumeton 33693-04-88. terbuthylazine 5915-41-39. secbumeton 26259-45-0

10. cyanazine 21725-46-2

Rtx®-OPPesticides2 30m, 0.25mm ID, 0.25µm (cat.# 11243)Sample: custom standard, 100ng/µL each componentInj.: 1.0µL splitless (hold 0.5 min.), 4mm single gooseneck inlet liner (cat.# 20904)Inj. temp.: 300°CCarrier gas: helium, constant pressure Flow rate: 1.0mL/min @ 80°C (27.22cm/sec) Oven temp.: 80°C (hold 1 min.) to 140°C @ 25°C/min. (hold 5 min.) to 165°C @

1°C/min. to 300°C @ 15°C (hold 1 min.) Det: Agilent 5971A GC/MSTransfer line temp.: 280°CScan range: 35-360amuIonization: EI

three simple words...PPlluuss OOnnee service exceeding your expectations in everything we do

IInnnnoovvaattiivvee products and processes

EExxeeccuuttiioonn in our commitment to deliver a product, technical support, orother needs

566-591_GCAPP.qxd 11/4/2004 3:33 PM Page 591

PCBs: Aroclor® 1242Rtx®-5 using GC Racer

GC_EV00581


GC_EV00582


GC_EV00583


Rtx®-5 15m, 0.32mm ID, 0.50µm (cat.# 10236)Inj.: 1µL splitless (hold 0.5 min.), Siltek™ Drilled

Uniliner® inlet liner (cat.# 21054-214.1)Conc.: 400 ppbInj. temp.: 250°C Carrier gas: hydrogenLinear velocity: head pressure at 5 psiOven temp.: 110°C (hold 1 min.), 60°C/min. to

300°C, (hold 5 min.)Det. temp.: 310°C

GC_EV00584

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PCB Mixtures (Aroclor®)


592

for more infoGG Racer TemperatureProgrammer is described onppaaggee 114499.

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 592

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LPCB Mixtures (Aroclor®)


593

PCBs: Aroclor® Quick ScreeningRtx®-5

Aroclor® 1221 Mix (cat.# 32007)

min. 2 4 6 8GC_EV00096


min. 2 4 6 8 10

GC_EV00097


min. 2 4 6 8 10GC_EV00099


min. 2 4 6 8 10GC_EV00100


min. 2 4 6 8 10

15m, 0.53mm ID, 0.50µm Rtx®-5 (cat.# 10237) 1.0µL direct injectionConc.: 50ppmOven temp.: 150°C to 300°C @ 12°C/min. (hold 5 min.)Inj. / det. temp.: 250°C / 310°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 150°CECD sensitivity: 16kHz full scale

GC_EV00101


min. 2 4 6 8

GC_EV00098

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 593

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594

PCBs: Aroclor® 1016Rtx®-CLPesticides & Rtx®-CLPesticides2

min. 2 4 6 8 10 12 14 16 18 20 22

30m, 0.32mm ID, 0.50µm Rtx®-CLPesticides (cat.# 11139). 30m, 0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324).0.5µL direct injection, 160pg on-column concentration. Oven temp.: 120°C (hold 1 min.) to 300°C @ 9°C/min. (hold 10 min.)Inj. temp.: 210°CDet.: ECD with anode purge, 310°CCarrier gas: helium

DCB

GC_EV00440

DCB

min. 2 4 6 8 10 12 14 16 18 20 22

Rtx®-CLPesticides

Rtx®-CLPesticides2

min. 2 4 6 8 10 12 14 16 18 20 22

PCBs: Aroclor® 1221Rtx®-CLPesticides & Rtx®CLPesticides2

min. 2 4 6 8 10 12 14 16 18 20

GC_EV00448

Rtx®-CLPesticides

Rtx®-CLPesticides2

DCB

DCB

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 594

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595

min. 2 4 6 8 10 12 14 16 18 20 22


min. 2 4 6 8 10 12 14 16 18 20 22

30m, 0.32mm ID, 0.50µm Rtx®-CLPesticides (cat.# 11139).0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324).0.5µL direct injection, 160pg on-column concentration.Oven temp.: 120°C (hold 1 min.) to 300°C @ 9°C/min. (hold 10 min.)Inj. temp.: 210°CDet.: ECD with anode purge, 310°CCarrier gas: helium

DCB

GC_EV00450

DCB

Rtx®-CLPesticides

Rtx®-CLPesticides2

min. 2 4 6 8 10 12 14 16 18 20 22


min. 2 4 6 8 10 12 14 16 18 20 22

DCB

GC_EV00451

DCB

Rtx®-CLPesticides

Rtx®-CLPesticides2

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 595

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596

min. 2 4 6 8 10 12 14 16 18 20 22


min. 2 4 6 8 10 12 14 16 18 20 22

GC_EV00453

30m, 0.32mm ID, 0.50µm Rtx®-CLPesticides (cat.# 11139).0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324).0.5µL direct injection of commercial Aroclor® mixtures, 160pg on-column concentration.Oven temp.: 120°C (hold 1 min.) to 300°C @ 9°C/min. (hold 10 min.)Inj. temp.: 210°CDet.: ECD with anode purge, 310°CCarrier gas: helium

Rtx®-CLPesticides

Rtx®-CLPesticides2

DCB

DCB


min. 2 4 6 8 10 12 14 16 18 20 22

GC_EV00454

DCB

DCB

Rtx®-CLPesticides

Rtx®-CLPesticides2

min. 2 4 6 8 10 12 14 16 18 20 22

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 596

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597


min. 2 4 6 8 10 12 14 16 18 20 22

GC_EV00456

30m, 0.32mm ID, 0.50µm Rtx®-CLPesticides (cat.# 11139).0.32mm ID, 0.25µm Rtx®-CLPesticides2 (cat.# 11324).0.5µL direct injection, 160pg on-column concentration.Oven temp.: 120°C (hold 1 min.) to 300°C @ 9°C/min. (hold 10 min.)Inj. temp.: 210°CDet.: ECD with anode purge, 310°CCarrier gas: helium

min. 2 4 6 8 10 12 14 16 18 20 22DC

B

DCB

Rtx®-CLPesticides

Rtx®-CLPesticides2

dependable executionTom consistently goes above and beyond to satisfy customers and completehis job duties. On a Friday morning last October, Tom arrived at 4:30am, tomake sure a number of GC columns went out in the mail and to ensure that hehad time for a training session, which was planned for the same day.

TToomm BBaarroonnee,, Applied Technology Manufacturing Technician

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 597

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PCB Mixtures


598

PCB CongenersRtx®-XLB

Column: Rtx®-XLB 30m, 0.32mm ID, 0.50µm (cat.# 12839)Sample: 200ppb each PCB congener in hexane (cat.# 32416), 200ppb

2,4,5,6-tetrachloro-m-xylene (SS) (cat.# 32027), 100ppb decachlorobiphenyl (IS) (cat.# 32289)

Inj.: 1.0µL splitless (hold 0.75 min.), 4mm Drilled Uniliner® inlet liner (cat.# 21055)Inj. temp.: 220°CCarrier gas: hydrogen, constant pressureLinear velocity: 66cm/sec. @ 120°COven temp.: 120°C (hold 0.5 min.) to 260°C @ 29°C/min. (hold 2.5 min.), to 330°C @

28°C/min. (hold 5 min.)Det.: ECD @ 320°C

Compound RT (min.)1. 2-chlorobiphenyl 3.862. 2,4,5,6 tetrachloro-m-xylene (SS) 4.533. 2,3-dichlorobiphenyl 4.834. 2,2',5-trichlorobiphenyl 5.175. 2,4',5-trichlorobiphenyl 5.636. 2,2',5,5'-tetrachlorobiphenyl 5.897. 2,2',3,5'-tetrachlorobiphenyl 6.098. 2,3',4,4'-tetrachlorobiphenyl 6.649. 2,2',4,5,5'-pentachlorobiphenyl 6.84

10. 2,2',3,4,5'-pentachlorobiphenyl 7.2411. 2,3,3',4',6-pentachlorobiphenyl 7.3912. 2,2',3,5,5',6-hexachlorobiphenyl 7.5113. 2,2',4,4',5,5'-hexachlorobiphenyl 8.1514. 2,2',3,4,5,5'-hexachlorobiphenyl 8.3515. 2,2',3,4,4',5'-hexachlorobiphenyl 8.6116. 2,2',3,4',5,5',6-heptachlorobiphenyl 8.7617. 2,2',3,4,4',5',6-heptachlorobiphenyl 8.8418. 2,2',3,4,4',5,5'-heptachlorobiphenyl 9.5019. 2,2',3,3',4,4',5-heptachlorobiphenyl 9.8620. 2,2',3,3',4,4',5,5',6-nonachlorobiphenyl 10.8721. decachlorobiphenyl (SS) 11.17

GC_EV00730

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 598

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LChlorinated Disinfection Byproducts


599

Chlorinated Disinfection ByproductsUS EPA Method 551.1Rtx®-5

1. chloroform2. 1,1,1-trichloroethane3. carbon tetrachloride4. trichloroacetonitrile5. trichloroethylene6. bromodichloromethane7. chloral hydrate8. dichloroacetonitrile9. 1,1-dichloro-2-propanone

10. 1,1,2-trichloroethane11. chloropicrin12. dibromochloromethane13. 1,2-dibromoethane (EDB)14. tetrachloroethylene15. bromochloroacetonitrile16. 1,1,1-trichloro-2-propanone17. bromoform18. dibromoacetonitrile19. 1,2,3-trichloropropane20. 4-bromofluorobenzene (IS)21. 1,2-dibromo-3-chloropropane

(DBCP)

30m, 0.25mm ID, 1.0µm Rtx®-5 (cat.# 10253)1.0µL split injection. 1ng on-column concentration.Oven temp.: 35°C (hold 22 min.) to 200°C @ 10°C/min.Inj./det. temp.: 200°C/290°CCarrier gas: heliumLinear velocity: 30cm/sec. @ 50°CECD sensitivity: 20kHz full scaleSplit ratio: 10:1

1

min. 10 20 30

2

3

4

5

6

7

8

9

10

11

1213

14

15

16

17

18

19

20

21

GC_EV00023

Chlorinated Disinfection ByproductsUS EPA Method 551.1Rtx®-200

1. chloroform2. 1,1,1-trichloroethane3. carbon tetrachloride4. trichloroacetonitrile5. trichloroethylene6. bromodichloromethane7. chloral hydrate8. dichloroacetonitrile9. 1,1-dichloro-2-propanone

10. 1,1,2-trichloroethane11. chloropicrin12. dibromochloromethane13. 1,2-dibromoethane (EDB)14. tetrachloroethylene15. bromochloroacetonitrile16. 1,1,1-trichloro-2-propanone17. bromoform18. dibromoacetonitrile19. 1,2,3-trichloropropane20. 4-bromofluorobenzene (IS)21. 1,2-dibromo-3-chloropropane

(DBCP)


1

min. 10 20 30

2

3

4

5/6

7 9

10

1213/11

14/8

15

16

17

19/18

20

21

GC_EV00024

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Dioxins


600

TCDD IsomersRtx®-2330

60m, 0.25mm ID, 0.20µm Rtx®-2330 (cat.# 10726)2.0µL splitless injection of TCDD isomers,approximately 0.5ng each isomer.

Splitless hold: 0.45 min.Oven temp.: 200°C (hold 1 min.) to 250°C @ 8°C/min. (hold 15

min.), to 275°C @ 15°C/min. (hold 5 min.)Inj. & det. temp.: 275°CCarrier gas: hydrogenLinear velocity: 40cm/sec.ECD sensitivity: 21 kHz full scale

1. 1,4,7,8-TCDD2. 2,3,7,8-TCDD3. 1,2,3,4-TCDD4. 1,2,3,75. 1,2,3,8-TCDD6. 1,2,7,8-TCDD7. 1,2,6,7-TCDD

GC_EV00108

min. 2 4 6 8 10 12 14 16 18 20

1

2

3

4, 5

6 7

TCDD IsomersRtx®-2330

GC_EV00109

min. 2 4 6 8 10 12 14 16 18 20 22

1

2

3

4, 5

6

7

60m, 0.32mm ID, 0.20µm Rtx®-2330 (cat.# 10727)1.5µL cold on-column injection of TCDD isomers,approximately 0.5ng each isomer.

Oven temp.: 200°C (hold 1 min.) to 240°C @ 3°C/min. (hold 6 min.), to 275°C @ 15°C/min. (hold 30 min.)

Inj. & det. temp.: 275°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 3.2cc/min.)ECD sensitivity: 5 kHz full scale

1. 1,4,7,8-TCDD2. 2,3,7,8-TCDD3. 1,2,3,4-TCDD4. 1,2,3,75. 1,2,3,8-TCDD6. 1,2,7,8-TCDD7. 1,2,6,7-TCDD

also availableFor Rtx®-Dioxin2 columnsdesigned specifically for dioxin/furan analysis, seeppaaggee 6666..

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 600

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LEndocrine Disruptors


601

Endocrine Disruptors: Alkyl PhenolsRtx®-5MS

30m, 0.25mm ID, 0.25µm Rtx®-5MS (cat.# 12623). Conc.: 5–10ng on-column Inj.: splitless, purge on @ 1 min.Oven temp.: 35°C (hold 1 min.) to 300°C @ 10°C/min. (hold 15 min.)Inj./det. temp.: 275°C/310°C Carrier gas: helium

1. tert-butyl phenol2. n-pentyl phenol3. n-hexyl phenol4. n-heptyl phenol5. tert-octyl phenol6. n-octyl phenol7. n-nonyl phenol8. bisphenol A

min. 12 14 16 18 20 22 24

1

2

3

4

5 6

7

8

GC_EV00046

Endocrine Disruptors: Phthalate EstersRtx®-5MS

30m, 0.25mm ID, 0.50µm Rtx®-5MS (cat #12638).100pg on-column injection MS-SIM. Oven temp.: 35°C (hold 1 min.) to 285°C @ 10°C/min.Pressure: 7.5psi constant pressure

1. dimethyl phthalate2. diethyl phthalate3. isobutyl phthalate4. dibutyl phthalate5. dipentyl phthalate6. dihexyl phthalate

7. benzylethyl phthalate8. diheptyl phthalate9. 2-ethylhexyl phthalate

10. cyclohexyl phthalate11. dioctyl phthalate

GC_EV00049min.18 20 22 24 26 28 30 32

12

34

5

6

7

8

9

10

11

Endocrine Disruptors:Butyl Tins (hexyl derivatives)Rtx®-5

min. 6 8 10 12 14

1

2

34

5

6

30m, 0.32mm ID, 0.50µm Rtx®-5 (cat.# 10239)500pg on-column direct injectionOven temp.: 100°C (hold 1 min.) to 285°C

@ 10°C/min.Inj./det. temp.: 250°CCarrier gas: heliumLinear velocity: 45cm/sec.Detector: FPD with 610nm filter

1. tetrapropyltin2. tetrabutyltin3. tributyltin4. dibutyltin5. tripentyltin6. monobutyltin

GC_EV00047

Endocrine Disruptors:Butyl Tins (hexyl derivatives)Rtx®-35

min. 6 8 10 12 14

1

2

34

56

30m, 0.32mm ID, 0.50µm Rtx®-35 (cat.# 10439)500pg on-column direct injectionOven temp.: 100°C (hold 1 min.) to 285°C

@ 10°C/min.Inj./det. temp.: 250°CCarrier gas: heliumLinear velocity: 45cm/sec.Detector: FPD with 610nm filter

1. tetrapropyltin2. tetrabutyltin3. tributyltin4. dibutyltin5. tripentyltin6. monobutyltin

GC_EV00048also availableButyl tins reference materialsavailable from Restek, seeppaaggee 441199..

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Explosives


602

ExplosivesRtx®-200

3

1

24

5

6

7

89

10

11,12

13

17

21

23

1416

15

18,19

20

22

min. 10 15 20 25 30 35 40 45 50 55

30m, 0.25mm ID, 0.25µm Rtx®-200 (cat.# 15023)1.0µL splitless injection of an explosives sample.Concentration 20ng/µL.

Oven temp.: 80°C (hold 2 min.) to 260°C @3°C/min. (hold 2 min.)

Injector temp.: 280°CDet.: MS, 300°CCarrier gas: heliumLinear velocity: 20cm/sec. set @ 80°CSplitless hold time: 0.6 min.

1. 2-nitrotoluene2. 3-nitrotoluene3. 4-nitrotoluene4. 2,3-diaminotoluene5. 2,6-diaminotoluene6. 2,4-diaminotoluene7. 1,4-dinitrobenzene8. 2,6-dinitrotoluene9. 2-amino-6-nitrotoluene

10. 1,3-dinitrobenzene11. 2,4-dinitrotoluene12. 2-amino-4-nitrotoluene

13. 2,3-dinitrotoluene14. 3,4-dinitrotoluene15. 3-nitrobiphenyl16. 2,4,6-trinitrotoluene17. 2,4,5-trinitrotoluene18. 4-amino-2,6-dinitrotoluene19. 2,3,4-trinitrotoluene20. 1,3-dinitronaphthalene21. 2,6-diamino-4-nitrotoluene22. 2-amino-4,6-dinitrotoluene23. 2,2'-dinitrobiphenyl

GC_EV00060


www.restek.com

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 602

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LExplosives


603

ExplosivesUS EPA Method 8095Rtx®-TNT & Rtx®-TNT2

1. nitrobenzene2. 2-nitrotoluene3. 3-nitrotoluene4. 4-nitrotoluene5. nitroglycerine6. 1,3-dinitrobenzene7. 2,6-dinitrotoluene8. 2,4-dinitrotoluene9. 3,4-dinitrotoluene (IS)

10. 1,3,5-trinitrobenzene11. trinitrotoluene12. PETN13. RDX14. 4-amino-2,6-dinitrotoluene15. 3,5-dinitroaniline16. 2-amino-4,6-dinitrotoluene17. tetryl18. HMX

6m, 0.53mm ID, 1.50µm Rtx®-TNT (cat.# 12998) Direct injection using a 1mm Siltek™ Uniliner® inlet liner (cat.# 21052-214.1). On-column conc.: 200-1000pg/compound. 8095 Calibration Mix A (cat.# 31607),

8095 Calibration Mix B (cat.# 31608), and 3,4-dinitrotoluene (cat.# 31452). Oven temp.: 80°C (hold 1 min.) to 180°C @ 10°C/min. to 300°C @

30°C/min. (hold 3 min.)Inj. temp.: 250°CDet.: ECD @ 330°C with anode purgeDead time: 4.4 sec.Head pressure: helium @ 3psi (20.7 KPa)Flow rate: helium @ 17mL/min. @ 80°C.

1 2 3

7

8

9

13

15

16

45

610

11

12

17 18

14

min. 2 4 6 8 10 12

GC_EV00406

Improved resolution ofnitroaromatic compounds suchas those listed in US EPAMethod 8095

6m, 0.53mm ID, 1.50µm Rtx®-TNT2 (cat.# 12999) Direct injection using a 1mm Siltek™ Uniliner® inlet liner (cat.# 21052-214.1). On-column conc.: 200-1000pg/compound. 8095 Calibration Mix A (cat.# 31607),

8095 Calibration Mix B (cat.# 31608), and 3,4-dinitrotoluene (cat.# 31452) Oven temp.: 80°C (hold 1 min.) to 180°C @ 10°C/min. to 300°C @

30°C/min. (hold 3 min.)Inj. temp.: 250°CDet.: ECD @ 330°C with anode purgeDead time: 4.4 sec.Head pressure: helium @ 3psi (20.7 KPa)Flow rate: helium @ 17mL/min. @ 80°C

1 2 35

8

9

16

15,13

4

11

10

12,14

1718

7

6

min. 2 4 6 8 10 12

GC_EV00407

Rtx®-TNT2

Rtx®-TNT

restekinnovation!

592-603_GCAPP.qxd 11/4/2004 1:54 PM Page 603

Gasoline Range Organics (GRO)


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604

Alaska Method AK101AARtx®-5Sil MS

PID

2

3

4

5

6,7

8 9

10

1112

13

14

15

16

18

min. 2 4 6 8 10 12 14 16

FID

1

12

1314

15

16,17 18

2

3

4

5

6,7

8 9

10

11

1. hexane2. benzene3. α,α,α-trifluorotoluene (SS)4. toluene5. ethylbenzene6. m-xylene7. p-xylene8. o-xylene9. isopropylbenzene

10. 4-bromofluorobenzene (SS)11. n-propylbenzene12. 1-ethyl-3-methylbenzene13. 1-ethyl-4-methylbenzene14. 1,3,5-trimethylbenzene15. 1-ethyl-2-methylbenzene16. 1,2,4-trimethylbenzene17. decane18. 1,2,3-trimethylbenzene

40m, 0.45mm ID, 1.50µm Rtx®-5Sil MS (cat.# 12798).Injection of 4-bromofluorobenzene (cat.# 30026); α,α,α-trifluorotoluene (cat.# 30048), decane, hexane, and Alaska aliphatic/aromatic GRO mix (cat.# 30461).Conc.: 10ppb in 5mL RO waterGC: Finnigan 9001Column flow: 9mL/min.Concentrator: Tekmar LSC-3000 Purge & Trap, BTEX trap Interface: direct with Siltek™ transfer lineOven temp.: 40°C (hold 2 min.) to 85°C @ 4°C/min. (hold 1 min.) to

225°C @ 40°C/min. (hold 2 min.)Det.: FID (280°C)/PID (200°C)Make-up flow rate: 15mL/min.

GC_EV00402

min. 2 4 6 8 10 12 14 16

SSttaattee ooff AAllaasskkaa UUSSTT MMoonniittoorriinngg((lliitt.. ccaatt..## 5599550033))

Reference materials for the state underground storage tank program

To help laboratories comply with and use applicable analytical procedures, Restek has carefully reviewed and fol-lowed state guidelines in formulating analytical reference materials for AK101, AK102, and AK103 UST monitoring.Calibration mixes described in this 6-page note are appropriate for Alaska methodology. Also described are retentiontime, surrogate, internal standard, fractionation check, matrix spike, and pattern recognition mixtures.

restekinnovation!Rtx®-5Sil MS column resolvesalkyl benzenes and branchedaromatic compounds listed inAlaska GRO Method AK101AA,for more accurate identifica-tion and quantitation.

free literaturefree literature

604-623_GCAPP.qxd 11/4/2004 3:18 PM Page 604



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L605

Volatile Petroleum Hydrocarbons (VPH)Massachusetts Department of Environmental Protection Rtx®-502.2

PID

min. 4 8 12 16 20 24 28 32

1. methanol2. methyl tert-butyl ether (60ng)3. benzene (20ng)4. toluene5. ethylbenzene (20ng)6. p+m-xylene (40ng ea.)7. o-xylene (40ng)8. 1,2,4-trimethylbenzene (40ng)9. naphthalene (40ng)

10. 2,5-dibromotoluene (SS)

2

1 3

4

5

6

7

8

910

Column: 105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910). Concentration: on-column at levels listed

Oven temp: 45°C to 90°C @ 3°C/min., to 140° @ 5°C/min., to 230°C @ 45°C/min. (hold 8 min.)

Carrier gas: helium @ 15mL/min. Tekmar Model LSC 2000 Trap: BTEX Purge: helium @ 40mL/min. for 11 min.Dry purge: 2 min. Desorb preheat: 245°CDesorb: 2 min. @ 250°CBake: 6 min. @ 260°C

Chromatograms courtesy of Severn Trent Laboratories, Burlington, VT.

1. methanol2. n-pentane (40ng)3. 2-methylpentane (60ng)4. methyl tert-butyl ether (60ng)5. 2,2,4-trimethylpentane (60ng)6. benzene (20ng)7. toluene (60ng)8. n-nonane (40ng)9. ethylbenzene (20ng)

10. p+m-xylene (40ng ea.)11. o-xylene (40ng)12. 1,2,4-trimethylbenzene (40ng)13. naphthalene (40ng)14. 2,5-dibromotoluene (SS)

FID

GC_EV00063

For VPH analysis on an Rtx®-502.2 column, use PID for aromatic compounds and FID for aliphatic compounds.

21

3

4

56

7

8 9

10

11

12

13

14

min. 4 8 12 16 20 24 28 32

OOppttiimmiizziinngg MMaassssaacchhuusseettttss VVoollaattiillee PPeettrroolleeuummHHyyddrrooccaarrbboonn GGCC AAnnaallyyssiiss ((lliitt.. ccaatt..## 5599115500))

Select a trap and analytical column that avoid retentionand quantification problems

Massachusetts VPH affords more reliable quantificationof volatile petroleum hydrocarbons, relative to older"analytical window" methods, and has been adopted byother states and in Canada. This 2-page note offersvaluable tips for selecting a trap and a capillary GC col-umn compatible with the methodology. Reference mixesspecifically designed for MA VPH are described.


604-623_GCAPP.qxd 11/4/2004 3:18 PM Page 605



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606

Texas UST: Alternate Boiling Point/Carbon Number Distribution MarkerRtx®-5

Rtx®-5 15m, 0.25 ID, 0.25µm (cat.# 10220)Sample: 200µg/mL Alternate BP/CN Distribution Marker Stock

Solution (cat.# 31639) in pentaneInj.: 0.5µL splitless (hold 0.2 min.), 4mm drilled Uniliner®

inlet liner (hole at bottom) (cat.# 20756)Inj. temp.: 285°CCarrier gas: helium, constant pressure Linear velocity: 27cm/sec. @ 30°COven temp.: 30°C (hold 1 min.) to 330°C @ 60°C/min. (hold 6

min.) with GC Racer for Agilent 5890 Series II GC (cat.# 23024)

Det.: FID @ 330°C

1. hexane (C6)2. octane (C8)3. decane (C10)4. dodecane (C12)5. hexadecane (C16)6. heneicosane (C21)7. octacosane (C28)8. pentatriacontane (C35)9. hexatriacontane (C36)

GC_EV00690

Rtx®-5 15m, 0.25mm ID, 0.25µm (cat.# 10220)Sample: 1000µg/mL unleaded gas and diesel fuel #2 composites

standard (cat.# 31483) in pentane250µg/mL 2-fluorobiphenyl standard (cat.# 31096) in dichloromethane250µg/mL 1-chlorooctane (cat.# 30084) in P&T methanol

Inj.: 0.5µL splitless (hold 0.2 min.), 4mm drilled Uniliner® inlet liner(hole at bottom) (cat.# 20756)

Inj. temp.: 285°CCarrier gas: helium, constant pressure Linear velocity: 27cm/sec. @ 30°COven temp.: 30°C (hold 1 min.) to 330°C @ 60°C/min. (hold 6 min.) with GC

Racer for Agilent 5890 Series II GC (cat.# 23024)Det.: FID @ 330°C

1. 1-chlorooctane (surrogate)2. 2-fluorobiphenyl (surrogate)

GC_EV00692

Texas UST: diesel/gas compositesRtx®-5

604-623_GCAPP.qxd 11/4/2004 3:18 PM Page 606



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L607

Texas UST: Alternate BP/CN Distribution MarkerRtx®-5

1. n-hexane(C6)2. n-octane(C8)3. n-decane(C10)4. n-dodecane(C12)5. n-hexadecane(C16)6. n-heneicosane(C21)7. n-octacosane(C28)8. n-pentatriacontane9. n-hexatriacontane(C36)

Rtx®-5 30m, 0.25mm ID, 0.50µm (cat.# 10238)Sample: 1µL Alternate Boiling Point/Carbon Number Distribution Marker Stock

Solution (cat.# 31639), 200µg/mL each component in pentaneCarrier gas: hydrogen @ 40cm/sec.Oven temp.: 40°C (hold 2 min.) to 330°C @ 10°C/min. (hold 5 min.)Inj. temp.: 250°C Det. temp.: 330°CDetector: FID

GC_EV00691

Wisconsin GRO Mix, plus C12Rtx®-502.2 6

1

2

3

4 7

9

105

8

min. 10 20 30

Standard used is GRO Mix (WISC) + C12(cat.# 30069):

1. 3-methylpentane2. 2,2,4-trimethylpentane (isooctane)3. benzene4. toluene5. ethylbenzene6. m-xylene7. o-xylene8. 1,2,4-trimethylbenzene9. dodecane

10. naphthalene

FID

105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910)GRO + C12 standard.Conc.: 200ppb each in 5mL of H2O.


Inj. / det. temp.: 200°C / 250°CCarrier gas: helium Flow rate: 10mL/min.Trap: Tenax, silica gel, charcoalPurge: 12 min. @ 40mL/min.Desorb preheat: 175°CDesorb temp.: 180°CDesorb time: 2 min.Desorb flow: 10mL/min.FID sensitivity: 16 x 10-11 AFS

10

9

8

76

5

4

3

GC_EV00071

PID

min. 10 20 30

604-623_GCAPP.qxd 11/4/2004 3:18 PM Page 607



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608

1 2

5

3

4

6

9

10

78

11

12

13 14

15, 16

17

18

20

21

22

23

24

25

CCoonnddiittiioonnss ffoorr aallll cchhrroommaattooggrraammss oonn tthhiiss ppaaggee::

30m, 0.45mm, 2.55µm Rtx®-VGC (cat.# 19408)each component 100ppb in 5mL of RO water, except tert-butanol 5000ppb; 2/1-methynapthalene 150ppb; ethylmethylbenzene 50ppb.Concentrator: Tekmar LSC-3100 Purge and TrapTrap: Vocarb 3000Purge: 11 min. @ 40mL/min. @ 35°CDry purge: 1 min. (MCS bypassed) GC: Finnigan 9001 Oven temp.: 40°C (hold 2 min.) to 130°C @ 6°C/min. (hold 0 min.) to 230°C

@ 30°C/min. (hold 2 min.). Carrier gas: helium @ ~8mL/min.Detector: Finnigan PID, make up 7mL min., purge 7mL/min., set @ 0.35mV,

base temperature 200°C.

1. 2-methylpentane2. 3-methylpentane3. methyl-tert-butyl ether4. tert-butanol5. diisopropyl ether6. ethyl-tert-butyl ether7. isooctane8. n-heptane9. benzene

10. tert-amyl-methyl ether11. α,α,α-trifluorotoluene12. toluene13. 1-chloro-3-fluorobenzene14. ethylbenzene15. m-xylene16. p-xylene17. o-xylene18. isopropylbenzene19. ethylmethylbenzene20. 1,3,5-trimethylbenzene21. 1,2,4-trimethylbenzene22. 4-bromochlorobenzene23. naphthalene24. 2-methylnaphthalene25. 1-methylnaphthalene

GC_EV00465

19

min. 5 10 15 20

OxygenatesRtx®-VGC

1

2

34 5

6/7

8

9

1. benzene2. α,α,α-trifluorotoluene3. toluene4. ethylbenzene5. chlorobenzene6. m-xylene7. p-xylene8. o-xylene9. 4-bromo-1-chlorobenzene

GC_EV00468

min. 0 5 10 15 20

BTEXRtx®-VGC

Unleaded GasolineRtx®-VGC

GC_EV00467

1. benzene2. toluene3. ethylbenzene4. m-xylene5. p-xylene6. o-xylene7. isopropylbenzene8. ethylmethylbenzene9. 1,2,3-trimethylbenzene

10. 1,2,4-trimethylbenzene11. naphthalene12. 2-methylnaphthalene13. 1-methylnaphthalene

see conditions below

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 608



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L609

BTEX Rtx®-502.2

10

9

7

5

64 8

3

2

1

GC_EV00073

FID

105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910)2500µL/mL injection of Unleaded Gasoline Composite Standard (cat.# 30081),10µL spiked into 5mL H2O.

Oven temp.: 40°C (hold 1 min.) to 100°C @ 5°C/min., to 240°C @ 8°C/min. (hold 8 min.)

Inj. / det. temp.: 200°C / 250°CCarrier gas: heliumFlow rate: 10mL/min.Trap: Tenax®, silica gel, charcoalPurge: 12 min. @ 40mL/min.Desorb preheat: 175°CDesorb temp.: 180°CDesorb time: 2 min.Desorb flow: 10mL/min.FID sensitivity: 16 x 10-11 AFS

1. 3-methylpentane2. 2,2,4-trimethylpentane (isooctane)3. benzene4. toluene5. ethylbenzene6. m-xylene7. o-xylene8. 1,2,4-trimethylbenzene9. dodecane

10. naphthalene

min. 10 20 30

10

9

8

7

53

PID

4 6

min. 10 20 30

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 609



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610

OxygenatesRtx®-VGC

30m, 0.25mm, 1.40µm Rtx®-VGC (cat.# 19415)10:1 split at injection port; 1mm ID linerCompounds at 100ppb in 5mL of RO water (unless otherwise noted)Oven program: 35°C (hold 14 min.) to 220°C @ 24°C/min. (hold 6 min.)Carrier gas: helium @ ~1mL/min. constant flowConcentrator: Tekmar LSC-3100 Purge and TrapTrap: Vocarb 3000Purge: 11 min. @ 40mL/min. @ ambient temperatureDry purge: 1 min. @ 40mL/min. (MCS bypassed using Silcosteel® tubing)Desorb preheat: 245°CDesorb: 250°C for 2 min., Flow 10mL/min.Bake: 260°C for 8 min.Interface: transfer line 0.32mm ID Siltek™ fused silicaDetector: Agilent 5973 MSScan range: 25-300amu

1. methanol (100,000ppb)2. ethanol (10,000ppb)3. 2-methylpentane4. 2-propanol (500ppb)5. 3-methylpentane6. hexane7. methyl tert-butyl ether8. tert-butanol (500ppb)9. diisopropyl ether

10. ethyl-tert-butyl ether11. isooctane12. benzene13. n-heptane14. tert-amyl methyl ether15. 1-butanol (500ppb)

16. α,α,α-trifluorotoluene17. toluene18. 1-chloro-3-fluorobenzene19. ethylbenzene20. m-xylene21. p-xylene22. o-xylene23. isopropylbenzene24. decane25. 1,3,5-trimethylbenzene26. 1,2,4-trimethylbenzene27. 4-bromochlorobenzene28. naphthalene29. 2-methylnaphthalene (150ppb)30. 1-methylnaphthalene (150ppb)

min. 2 6 10 14 18 22 26

1

2

3

4

5

6,7

9

8

10

1211

15

13

14

16

1817

19

20,21

23

22

2425

26

27 2829

30

Resolve gasoline components and common oxygenated additives!

GC_EV00401

RReessoollvviinngg OOxxyyggeennaatteess ffrroomm GGaassoolliinnee AAddddiittiivveess,, UUssiinngg aann RRttxx®®--VVGGCC GGCC CCoolluummnn((lliitt.. ccaatt..## 5599334455))Avoid coelution of compounds that share quantification ionsSuccessful analysis of oxygenates in environmental samples depend on the ability of the analytical column toresolve the oxygenates from early-eluting alkanes, alkenes, and alkynes. Nonpolar columns are used for thisapplication, but they are incompatible with polar compounds, and thus can give broad peaks and reduced sam-ple capacity for the alcohols. The unique polarity of Rtx®-VGC columns makes them ideal for oxygenates analy-sis. Example chromatograms with both PID and MS detection are shown in this 4-page note; purge and trapconditions also are discussed.

restekinnovation!

free literature

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 610



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L611

Rtx®-5 15m, 0.25mm ID, 0.25µm (cat.# 10220)Sample: 500µg/mL Unleaded Gasoline

Standard (unweathered) (cat.# 30096) in methylene chloride

Inj.: 1.0µL splitless (hold 0.5 min.), 4mm single gooseneck inlet liner (cat.# 20904)

Inj. temp.: 260°CCarrier gas: helium, constant pressure Linear velocity: 76cm/sec. @ 50°COven temp.: 35°C (2 min.) to 330°C

@ 60°C/min. using GC RacerDet.: FID @ 330°C

Unleaded GasolineRtx®-5

GC_EV00671

Rtx®-5 15m, 0.25mm ID, 0.25µm (cat.# 10220)Sample: 500µg/mL Jet Fuel A

standard (cat.# 31215) in methylene chloride


Inj. temp.: 260°CCarrier gas: helium, constant pressure Linear velocity: 76cm/sec. @ 50°COven temp.: 50°C (1 min.) to 330°C @

60°C/min. (hold 0.5 min.)using GC Racer

Det.: FID @ 330°C

Jet FuelRtx®-5

GC_EV00670

FFAASSTT analyses, using a GCRacer temperature program-mer described on ppaaggee 114499.

for more info

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 611


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612 Diesel Range Organics (DRO)

Rtx®-5 15m, 0.25mm ID, 0.25µm (cat.# 10220)Sample: 500µg/mL motor oil in methylene chlorideInj.: 1.0µL splitless (hold 0.5 min.),

4mm single gooseneck inlet liner (cat.# 22405)Inj. temp.: 260°CCarrier gas: helium, constant pressure Linear velocity: 76cm/sec. @ 50°COven temp.: 50°C (1 min.) to 330°C @ 60°C/min. (hold 0.5 min.)

using GC RacerDet.: FID @ 330°C

Motor OilRtx®-5

GC_EV00675

All 3 chromatograms on pages 612-613 were run at equivalent conditions—all runs less than 10 min.


www.restek.com


for more info

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 612

www.restek.com

Diesel Range Organics (DRO)


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L613

Rtx®-5 15m, 0.25mm ID, 0.25µm (cat.# 10220)Sample: 500µg/mL Diesel Fuel Composite standard (cat.# 31093) in methylene chlorideInj.: 1.0µL splitless (hold 0.5 min.), 4mm single gooseneck inlet liner (cat.# 20904)Inj. temp.: 260°CCarrier gas: helium, constant pressure Linear velocity: 76cm/sec. @ 50°COven temp.: 50°C (1 min.) to 330°C @ 20°C/min.Det.: FID @ 330°C

Diesel FuelRtx®-5

GC_EV00669

Rtx®-5 15m, 0.25mm ID, 0.25µm (cat.# 10220)Sample: 500µg/mL Diesel Fuel Composite

standard (cat.# 31093) in methylene chloride


Inj. temp.: 260°CCarrier gas: helium, constant pressure Linear velocity: 76cm/sec. @ 50°COven temp.: 50°C (1 min.) to 330°C @ 60°C/min.

(hold 0.5 min.) using GC RacerDet.: FID @ 330°C

Diesel FuelRtx®-5

GC_EV00668


for more info

20°C/min.temperatureprogram

60°C/min.temperatureprogram

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 613

Diesel Range Organics (DRO)


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DRO MixRtx®-5

1. C102. C123. C144. C165. C186. C207. C228. C249. C26

10. C28

12 3

4

10987

6

5

30m, 0.53mm ID, 1.0µm Rtx®-5 (cat.# 10255)Direct injection of DRO Mix (cat.# 31064) Conc.: 50ng/µL


Inj. / det. temp.: 280°C / 310°CCarrier gas: helium @ 65cm/sec.FID sensitivity: 8 x 10-11 AFS

min. 5 10 15 20 25GC_EV00069

Diesel FuelRtx®-5

30m, 0.53mm ID, 1.0µm Rtx®-5 (cat.# 10255)Direct injection of Diesel Fuel #2 Composite (cat.# 31093) Conc.: 500ng/µL


Inj. / det. temp.: 280°C / 310°CCarrier gas: helium @ 65cm/sec.FID sensitivity: 8 x 10-11 AFS

min. 5 10 15 20 25GC_EV00070

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 614

Glycols


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L615

GlycolsRtx®-Wax

min. 1 1.50 2 2.50 3 3.50 4 4.50 5 5.50 6 6.50 7 7.50 8 8.50 9 9.50 10

1

2

1. propylene glycol2. ethylene glycol

30m, 0.53mm ID, 1.0µm Rtx®-Wax (cat.# 12455)Inj.: directInj. / det. temp.: 220°C / 270°COven temp.: 80°C (hold 1 min.) to 120°C

@ 8°C/min. (hold 3 min.)10psi pressureStd. = 100µg/mL

GC_EV00444

GlycolsRtx®-200

min. 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00

1

2

1. ethylene glycol2. propylene glycol

30m, 0.53mm ID, 1.0µm Rtx®-200 (cat.# 15055)Inj.: directInj. / det. temp.: 220°C / 270°COven temp.: 80°C (hold 1 min.) to 200°C

@ 8°C/min. (hold 3 min.)10psi pressureStd. = 100µg/mL

GC_EV00445

TTeecchhnniiqquueess ttoo OOppttiimmiizziinngg GGCC AAnnaallyyssiiss ooff EEtthhyylleennee GGllyyccooll iinn WWaatteerr

((lliitt.. ccaatt..## 5599118877))

Avoid the problems that aqueous samples can create

Direct injections of water-based samples, such as samples con-taining ethylene glycol, can cause problems for chromatogra-phers. This 4-page note alerts analysts to the potential for poorpeak shape, sample carryover, and FID flameout, and describesapproaches to follow to avoid these problems. With care, detec-tion limits of 1-10ppm ethylene glycol can be attained.


604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 615

Glycols


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GlycolsStabilwax®

30m, 0.53mmID, 1.0µm Stabilwax® (cat.# 10655)1.0µL direct injection of glycol mix, open-top Uniliner® liner without wool(cat.# 20843-205)Conc.: 150ppmSolvent: water/methanol (50:50)

Oven temp.: 80°C to 200°C @ 8°C/min. (hold 10 min.)Septa Purge: 5.0cc/min.Carrier Gas: heliumLinear Velocity: 50cm/sec.Column Flow Rate: 6.9mL/min.Detector: FID/270°CMake up Gas Flow: 45cc/min.

1. 1,2-propylene glycol2. ethylene glycol3. 1,3-butylene glycol4. 1,3-propylene glycol5. 1,4-butylene glycol6. diethylene glycol7. glycerol

GC_EV00476

30m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10655)1.0µL of direct injection of glycols in water. Open-topUniliner®, liner without wool (cat.# 20843-205).Conc.: 100ppm


Inj./det. temp.: 225°C/250°CCarrier gas: heliumLinear velocity: 50cm/sec.Detector: FIDSeptum Purge: 5.0cc/min.

min.8.0 9.0 10.0 11.0

~~

1. propylene glycol2. ethylene glycol

1

2

GC_EV00546

GlycolsStabilwax®

GlycolsRtx®-BAC1 & Rtx®-BAC2

1. ethylene glycol2. propylene glycol

Rtx®-BAC1 Rtx®-BAC2

30m, 0.32mm ID, 1.8µm Rtx®-BAC1 (cat.# 18003)30m, 0.32mm ID, 1.2µm Rtx®-BAC2 (cat.# 18002)0.5µL split injectionConc.: 1%Solvent: methanol

Oven temp.: 100°C to 240°C @ 5°C/min. (hold 5 min.)Inj. temp.: 240°CInj. mode: 100:1 splitLiner: Agilent 4mm single gooseneckSepta purge: 5.0cc/min.Carrier gas: helium, constant pressurePressure: 12psiLinear velocity: 37cm/sec.Column flow rate: 2.1mL/min.Detector: FID/240°CMake-up gas flow: 40cc/min.

GC_EV00474

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 616

Haloacetic Acids


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L617

Haloacetic AcidsUS EPA Method 552.2Rtx®-5


1

min. 10 12 14 16 18 20 22 24 26

23

4

56/7

8

9

10

11

12

1. monochloroacetic acid (MCAA)2. monobromoacetic acid (MBAA)3. dichloroacetic acid (DCAA)4. dalapon5. 1,2,3-trichloropropane (I.S.)6. bromochloroacetic acid (BCAA)7. trichloroacetic acid (TCAA)8. dibromoacetic acid (DBAA)9. bromodichloroacetic acid (BDCAA)

10. 2,3-dibromopropionic acid (Surr.)11. chlorodibromoacetic acid (CDBAA)12. tribromoacetic acid (TBAA)

(All compounds derivatized)

GC_EV00025

Haloacetic AcidsUS EPA Method 552.2Rtx®-200


1

min. 10 12 14 16 18 20 22 24 26

2/3

4

5

6

8

9

10

11

12

7

1. monochloroacetic acid (MCAA)2. monobromoacetic acid (MBAA)3. dichloroacetic acid (DCAA)4. dalapon5. 1,2,3-trichloropropane (I.S.)6. bromochloroacetic acid (BCAA)7. trichloroacetic acid (TCAA)8. dibromoacetic acid (DBAA)9. bromodichloroacetic acid (BDCAA)

10. 2,3-dibromopropionic acid (Surr.)11. chlorodibromoacetic acid (CDBAA)12. tribromoacetic acid (TBAA)

(All compounds derivatized)

GC_EV00026

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 617

Haloacetic Acids


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618

Haloacetic AcidsUS EPA Method 552.2Rtx®-CLPesticides & Rtx®-CLPesticides2

min. 1 2 3 4 5 6 7 8 9 10 11

1

2 3

4

C

5

6

7

8

9

10 11 12Rtx®-CLPesticides30m, 0.32mm ID, 0.50µm (cat.# 11139)

RT1. monochloroacetic acid MCAA 4.412. monobromoacetic acid MBAA 5.753. dichloroacetic acid DCAA 5.924. dalapon DL 6.355. 1,2,3-trichloropropane IS 6.976. trichloroacetic acid TCAA 7.207. bromochloroacetic acid BCAA 7.318. dibromoacetic acid DBAA 8.439. bromodichloroacetic acid BDCAA 8.52

10. chlorodibromoacetic acid CDBAA 9.6811. 2,3-dibromopropionic acid SURR 9.8212. tribromoacetic acid TBAA 10.73

C = contaminant in solventCompounds derivatized to esters

GC_EV00399

2µL splitless injection of custom haloacetic acid methyl derivatives std. [20-200µg/mL] mix in MTBEOven temp.: 40°C (hold 4 min.) to 200°C @ 14°C/min.Inj. temp.: 200°CCarrier gas: heliumLinear velocity: 46cm/sec.ECD temp.: 300°C

min. 1 2 3 4 5 6 7 8 9 10 11

1

2

3

4+C

5

6

7

8

9

10 11 12


RT1. monochloroacetic acid MCAA 3.702. monobromoacetic acid MBAA 5.433. dichloroacetic acid DCAA 5.504. dalapon DL 5.786. trichloroacetic acid TCAA 6.835. 1,2,3-trichloropropane IS 6.987. bromochloroacetic acid BCAA 7.179. bromodichloroacetic acid BDCAA 8.438. dibromoacetic acid DBAA 8.49

10. chlorodibromoacetic acid CDBAA 9.8011. 2,3-dibromopropionic acid SURR 9.8612. tribromoacetic acid TBAA 11.04

C = contaminant in solvent(Compounds derivatized to esters)

GC_EV00400

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 618

Air Analysis


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L619

US EPA TO-14 CompoundsRtx®-1

1. dichlorodifluoromethane2. chloromethane3. 1,2-dichlorotetrafluoroethane4. vinyl chloride5. bromomethane6. chloroethane7. trichlorofluoromethane8. 1,1-dichloroethene9. methylene chloride

10. 3-chloropropene11. 1,1,2-trichloro-1,2,2-trifluoroethane12. 1,1-dichloroethane13. cis-1,2-dichloroethene14. chloroform15. 1,2-dichloroethane16. 1,1,1-trichloroethane17. benzene18. carbon tetrachloride19. 1,2-dichloropropane20. trichloroethene

21. cis-1,3-dichloropropene22. trans-1,3-dichloropropene23. 1,1,2-trichloroethane24. toluene25. 1,2-dibromoethane26. tetrachloroethene27. chlorobenzene28. ethylbenzene29. m-xylene30. p-xylene31. styrene32. o-xylene33. 1,1,2,2-tetrachloroethane34. 4-methyltoluene35. 1,3,5-trimethylbenzene36. 1,2,4-trimethylbenzene37. 1,3-dichlorobenzene38. 1,4-dichlorobenzene39. 1,2-dichlorobenzene40. 1,2,4-trichlorobenzene41. hexachlorobutadiene

1

2

3

4

5

6

7

8

910

12

13

14

11

16

1517

18

19

20

21

22

23

24

25

41

40

39

38

37

3635

34

32,33

31

29,30

28

27

26

min. 10 15 20 25 30 35

60m, 0.32mm ID, 3.0µm Rtx®-1 (cat.# 10187)5mL of 2ppmv TO-14 standard.

Oven temp.: 30°C (hold 4 min.) to 250°C @ 7°C/min. (hold 15 min.).Detector: MSDet. temp.: 250°CCarrier gas: heliumLinear velocity: 21cm/sec. set @ 30°CIonization: EIScan range: 34-280 AMU

Preconcentrator conditions:Nutech 3550 A PreconcentratorCryotrap temp.: -160°CCryotrap desorb temp.: 150°CCryofocusing unit temp.: -190°CCryofocusing desorb temp.: 150°C

GC_EV00018

Ozone PrecursorsRtx®-1

min. 5 10 15 20 25 30 35

321

54

6

78

910

1112

1314

1615

17

18 19 20 21 22

2423

25 26 27 2829

30

31,32

3334 35

36,37

38

1. ethylene2. acetylene3. ethane4. propylene5. propane6. isobutane7. 1-butene8. n-butane9. trans-2-butene

10. cis-2-butene11. isopentane12. 2-methyl-1-butene13. n-pentane14. trans-2-pentene15. cis-2-pentene16. cyclopentane17. isohexane18. 3-methylpentane19. n-hexane20. methylcyclopentane21. benzene22. isoheptane23. 2,3-dimethylpentane

24. 3-methylhexane25. 2,2,4-trimethylpentane26. n-heptane27. methylcyclohexane28. 2,2,3-trimethylpentane29. toluene30. ethylbenzene31. m-xylene32. p-xylene33. o-xylene34. n-nonane35. n-propylbenzene36. p-ethyltoluene37. 1,3,5-trimethylbenzene38. 1,2,4-trimethylbenzene

60m, 0.32mm ID, 3.0µm Rtx®-1 (cat.# 10187)0.5L of C2-C9 gas standard cryogenically concentrated; 15nL/component desorbed onto column.

Oven temp.: -60°C (hold 5 min.) to 100°C @ 8°C/min., to 150°C @ 6°C/min., then to 240°C @ 8°C/min.

Carrier gas: heliumLinear velocity: 30cm/sec. (flow rate: 1.8cc/min.)FID sensitivity: 64 x 10-12 AFS

GC_EV00019Permission to publish this chromatogram granted by Radian Corporation.

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 619

Air Analysis


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620

Air ToxinsRtx®-502.2

Permission to publish this chromatogram granted by Tekmar Company.

60m, 0.32mm ID, 1.8µm Rtx®-502.2 (cat.# 10920)500mL of 10ppbv standard. Concentrated on an AEROCAN® 6000 using a glass bead trap at 165°Cthen desorbed at 200°C for 4 min. @ 1mL/min. Cryofocused @ -175°C then desorbed @ 150°C.

Oven temp.: 35°C (hold 6 min.) to 120°C @ 15°C/min., then to 200°C @ 5°C/min., then to 220°C @ 25°C/min. (hold 10 min.).

Det. & det. temp.: Agilent-5971A GC/MS, 280°CCarrier gas: helium @ 1mL/min.Linear velocity: 20cm/sec.Scan range: 28-260 AMUSolvent delay: 4 min.

1. chlorodifluoromethane2. dichlorodifluoromethane3. dichlorotetrafluoroethane4. chloromethane5. butane6. vinyl chloride7. 1,3-butadiene8. acetaldehyde9. bromomethane

10. chloroethane11. trichlorofluoromethane12. isopropanol13. acetone14. 1,1-dichloroethene15. acetonitrile16. dichloromethane17. acrylonitrile18. 1-propanol

19. trans-1,2-dichloroethene20. 1,1-dichloroethane21. methyl ethyl ketone22. cis-1,2-dichloroethene23. methacrylonitrile24. chloroform25. bromochloromethane26. tetrahydrofuran27. 1,1,1-trichloroethane28. n-butanol29. heptane30. 1,2-dichloroethane31. benzene32. 1,4-difluorobenzene33. trichloroethene34. ethyl methacrylate35. 1,2-dichloropropane36. 1,4-dioxane

37. bromodichloromethane38. 4-methyl-2-pentanone39. octane40. toluene41. 2-hexanone42. 1,1,2-trichloroethane43. tetrachloroethene44. dibromochloromethane45. 1,2-dibromoethane46. chlorobenzene-d547. chlorobenzene48. m-xylene49. p-xylene50. 2-heptanone51. styrene52. o-xylene53. isopropylbenzene54. bromoform

55. 1,1,1,2-tetrachloroethane56. 4-bromofluoromethane57. n-propylbenzene58. 1,3,5-trimethylbenzene59. α-methylstyrene60. tert-butylbenzene61. 1,2,4-trimethylbenzene62. sec-butylbenzene63. 1,3-dichlorobenzene64. 1,4-dichlorobenzene65. butylbenzene66. 1,2-dichlorobenzene67. dodecane68. dibromochloropropane69. 1,2,4-trichlorobenzene70. hexachlorobutadiene71. naphthalene

min. 5 10 15 20 25 30

1

2

3

4

5

76

8 9

10

11

13

12

14

1518

19

2021

1617

22,23

24

25,26

27

28

29

31

30

32

33

35

34

38

39

40 41

42

43

44

36,37

45

46

47

48,49

50

71

7069

68

67

6665

63

64

6260

59

58

57

56

5554

53

51,52

61

GC_EV00022

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 620

Air Analysis


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NTA

L621

Rtx®-1 60m, 0.32mm ID, 1.0µm (cat.# 10157).200mL of 10ppbv TO-15 standard (cat.# 34436), injected into TO-Can™ canister andhumidified to 70% RH. Concentrator: Nutech 3550 Preconcentrator

200mL of sample concentrated at 160°C, thermally desorbed at 150°C, and cryofocused at 185°C.

Oven temp.: 30°C (hold 4 min.) to 175°C @ 9°C/min. to 220°C @ 40°C/min.Carrier gas: helium @ 1.2mL/min.Det.: Agilent 5971 MSScan range: 35-265amu

4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 min.

1

3

45

6

7

8

9/10

11

12

1314

15

16

1718

1920

21

22/2324

25

26

27

28

29

30

31

32

333435/36

37

38

39

40

41 42

4344

45

46

47

48

49

50

51

52

53

54

55

56

5758

59

60 61

62

63

BFB

GC_EV00379

2

Chromatogram courtesy of Gina Maio, Severn Trent Laboratories, Inc., Burlington, VT.

1. dichlorofluoromethane2. chloromethane3. dichlorotetrafluoroethane4. vinyl chloride5. 1,3-butadiene6. bromomethane7. chloroethane8. bromoethene9. acetone

10. trichlorofluoromethane11. isopropyl alcohol12. 1,1-dichloroethene13. methylene chloride14. 3-chloropropene15. carbon disulfide16. Freon® TF17. trans-1,2-dichloroethene18. 1,1-dichloroethane19. methyl tert-butyl ether20. methyl ethyl ketone21. cis-1,2-dichloroethene22. bromochloromethane (IS)

23. n-hexane24. chloroform25. tetrahydrofuran26. 1,2-dichloroethane27. 1,1,1-trichloroethane28. benzene29. carbon tetrachloride30. cyclohexane31. 1,4-difluorobenzene (IS)32. 1,2-dichloropropane33. bromodichloromethane34. trichloroethene35. 1,4-dioxane36. 2,2,4-trimethylpentane37. n-heptane38. cis-1,3-dichloropropene39. methyl isobutyl ketone40. trans-1,3-dichloropropene41. 1,1,2-trichloroethane42. toluene43. methyl butyl ketone44. dibromochloromethane

45. 1,2-dibromoethane46. tetrachloroethene47. chlorobenzene-d5 (IS)48. chlorobenzene49. ethylbenzene

50a. m-xylene50b. p-xylene51. bromoform52. styrene53. 1,1,2,2-tetrachloroethane54. o-xylene55. 2-chlorotoluene56. 4-ethyltoluene57. 1,3,5-trimethylbenzene58. 1,2,4-trimethylbenzene59. 1,3-dichlorobenzene60. 1,4-dichlorobenzene61. 1,2-dichlorobenzene62. 1,2,4-trichlorobenzene63. hexachlorobutadiene

US EPA TO-14/TO-15 CompoundsRtx®-1

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 621

Air Analysis


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US EPA TO-15 CompoundsRtx®-1

Rtx®-1 30m, 0.32mm ID, 1.0µm (cat.# 10157)Sample: TO-15 standard (cat.# 34436) humidified to 33% RH in a 6L SilcoCan™

canister (cat.# 24182)Concentrator: Nutech 3550A cryogenic concentrator; 300mL sample concentrated at

-160°C, thermally desorbed at 150°C, cryofocused at -185°C, thermally desorbed to column at 150°C

Carrier gas: helium, constant flowFlow rate: 1.2mL/min.Oven temp.: 30°C (hold 4 min.) to 175°C @ 8°C/min., to 220°C @

20°C/min. (hold 2 min.)Det.: MSTransfer line

temp.: 150°CScan range: 35–280 amuIonization: EIMode: scan

1. propylene2. Freon®-12 (dichlorodifluoromethane)3. chloromethane4. Freon®-114 (dichlorotetrafluoroethane)5. vinyl chloride6. 1,3-butadiene7. bromomethane8. chloroethane9. carbon disulfide

10. acetone11. Freon®-11 (trichlorofluoromethane)12. isopropyl alcohol13. 1,1-dichloroethene14. methylene chloride15. Freon®-113 (1,1,2-trichloro-1,2,2-trifluoroethane)16. trans-1,2-dichloroethene17. 1,1-dichloroethane18. methyl tert-butyl ether19. vinyl acetate20. methyl ethyl ketone21. cis-1,2-dichloroethene

22. hexane23. chloroform24. ethyl acetate25. tetrahydrofuran26. 1,2-dichloroethane27. 1,1,1-trichloroethane28. benzene29. carbon tetrachloride30. cyclohexane31. 1,2-dichloropropane32. trichloroethylene33. bromodichloromethane34. 1,4-dioxane35. heptane36. cis-1,3-dichloropropene37. methyl isobutyl ketone38. trans-1,3-dichloropropene39. 1,1,2-trichloroethane40. toluene41. methyl butyl ketone42. dibromochloromethane

43. 1,2-dibromoethane44. tetrachloroethylene45. chlorobenzene46. ethylbenzene47. p-xylene48. m-xylene49. bromoform50. styrene51. o-xylene52. 1,1,2,2-tetrachloroethane53. 4-ethyltoluene54. 1,3,5-trimethylbenzene55. 1,2,4-trimethylbenzene56. 1,3-dichlorobenzene57. benzyl chloride58. 1,4-dichlorobenzene59. 1,2-dichlorobenzene60. 1,2,4-trichlorobenzene61. hexachloro-1,3-butadiene

GC_AR00748


www.restek.com

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 622

www.restek.com

Air Analysis


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NTA

L623

Massachusetts APH MixRtx®-1

Rtx®-1 60m, 0.32mm ID, 1.0µm (cat.# 10157)Sample: Massachusetts APH Mix, (cat.# 34446)Concentrator: Nutech 3550A Air Preconcentrator, 100mL of a 40ppbv standard

concentrated at -160°C, thermally desorbed at 150°C and cryofocused at -185°C.

Carrier gas: helium Flow rate: 1mL/min.

Oven temp.: 35°C (hold 1 min.) to 220°C @ 8°C/min. Det: MS, HP5971Transfer line temp.: 250°CScan range: 35-280amuIonization: EIMode: scan

1. 1,3-butadiene2. isopentane3. methyl tert-butyl ether4. hexane5. benzene6. cyclohexane7. 2,3-dimethylpentane8. heptane9. toluene-D8

10. toluene11. octane12. ethylbenzene13. 2,3-dimethylheptane

14a. m-xylene 14b. p-xylene15. o-xylene16. nonane17. isopropylbenzene18. 1-methyl-3-ethylbenzene19. 1,3,5-trimethylbenzene20. decane21. 1,2,3-trimethylbenzene22. p-isopropyltoluene23. butylcyclohexane24. undecane25. dodecane

GC_AR00697

a plus 1 story“Restek is a first class company with excellent customer service. Courtesy is always extended to both my com-pany and myself when communicating with Restek employees”

RRiicchh WWhhiittnneeyy,, Mass Spec Manager, ProChem Analytical

604-623_GCAPP.qxd 11/4/2004 3:19 PM Page 623

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624 Antioxidants, Preservatives


Rtx®-50, 30m, 0.53mm ID, 0.50µm (cat.# 10540)Sample: 50ppm each in methanol Inj.: 1.0µL direct, gooseneck splitless inlet liner, 4mm (cat.# 20798)Inj. temp.: 280°CCarrier gas: helium, constant pressure Linear velocity: 60cm/sec. @ 50°COven temp.: 50°C to 240°C @15°C/min. (hold 3 min.)Det.: FID @280°C

BHA and BHTRtx®-50

GC_FF00630

1. BHA2. BHT

Rtx®-20, 30m, 0.53mm ID, 0.5µm (cat.# 10340)Sample: 1mg/mL each component in isooctane Inj.: 1.0µL split (split ratio 20:1), 4mm inlet liner (cat.# 20814)Inj. temp.: 320°CCarrier gas: hydrogen, constant flow Flow rate: 5.2mL/min.Oven temp.: 270°C to 290°C @ 2°C/min.

290°C to 320°C @ 10°C/min. (1 min. hold)Det.: FID @ 320°C

Tocopherols and TocotrienolsRtx®-20

GC_FF00634

1. δ-tocopherol2. β-tocopherol3. γ-tocopherol4. dl-δ-tocotrienol5. α-tocopherol6. dl-β-tocotrienol7. dl-γ-tocotrienol8. hexadecyl hexadecanoate9. dl-α-tocotrienol


www.restek.com

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 624

www.restek.com

GC

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S625Chiral Separations


Rosemary OilRt-βDEXsm™

1. (-/+)α-pinene2. (+/-)camphene3. (+/-)β-pinene4. (-/+)limonene5. eucalyptol (1,8-cineole)6. (-/+)linalool7. (+/-)camphor8. (-/+)terpinen-4-ol9. (+/-)isoborneol

10. (+/-)borneol11. (+/-)α-terpineol

30m, 0.32mm ID, 0.25µmRt-βDEXsm™ (cat.# 13104) Oven temp.: 40°C (hold 1 min.) to 200°C

@ 2°C/min. (hold 3 min.) Carrier gas: hydrogenLinear velocity: 80cm/sec.Det.: FID set @ 220°C

min. 0 10 20 30 40

1

2

3

3

4

4

5

6

7

8

9

10

1111109

GC_FF00147

Geranium Oil (Commercial)Rt-βDEXsa™

1a. (+)-(2R,4S)-cis-rose oxide1b. (-)-(2S,4R)-cis-rose oxide2a. (-)-(2R,4R)-trans-rose oxide2b. (+)-(2S,4S)-trans-rose oxide3. isomenthone4. menthone5a. (-)-(R)-linalool5b. (+)-(S)-linalool6a. (-)-(S)-β-citronellol6b. (+)-(R)-β-citronellol7. geraniol8. citronellyl formate

30m, 0.25mm ID, 0.25µm Rt-βDEXsa™ (cat.# 13109)Oven temp.: 60°C to 110°C @ 1°C/min. (hold 30 min.) Carrier gas: hydrogenLinear velocity: 40cm/sec. set @ 60°CDet.: FID set @ 220°C

min. 20 30 40 50 60

1a1b

2a2b

3

4

5a 5b

6a 6b

7

8

GC_FF00148

Lemon OilRt-βDEXsm™

1. (-/+)α-pinene2. (+/-)β-pinene3. (-/+)limonene

30m, 0.32mm ID, 0.25µmRt-βDEXsm™ (cat.# 13104)Oven temp.: 40°C (hold 1 min.) to

200°C @ 2°C/min. (hold 3 min.) Carrier gas: hydrogenLinear velocity: 80cm/sec.Det.: FID set @ 220°C

min. 10 15 20 25 30 35 40 45

1

12

2

3

3

GC_FF00146

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 625

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626 Chiral Separations


Menthol - Rt-βDEXsp™

30m, 0.32mm ID, 0.25µmRt-βDEXsp™ (cat.# 13110)Oven temp.: 60°C (hold 1 min.) to

200°C @ 2°C/min. Carrier gas: hydrogenLinear velocity: 80cm/sec. set @ 40°CDet.: FID set @ 220°C

1

min. 30

21. (-)menthol2. (+)menthol

GC_FF00149

Irone Isomers - Rt-βDEXcst™

1. (-)-(2R,6R)-trans-α-irone2. (+)-(2S,6S)-trans-α-irone3. (+)-(2R,6R)-trans-γ-irone4. (-)-(2S,6S)-trans-γ-irone5. (+)-(2R,6S)-cis-α-irone6. (+)-(2R,6S)-cis-γ-irone7. (-)-(2S,6R)-cis-γ-irone8. (-)-(2S,6R)-cis-α-irone9. (+)-(2R)-β-irone

10. (-)-(2R)-β-irone

30m, 0.32mm ID, 0.25µm Rt-βDEXcst™ (cat.# 13108)Oven temp.: 40°C (hold 1 min.) to

230°C @ 2°C/min. (hold 3 min.) Carrier gas: hydrogenLinear velocity: 80cm/sec. set @ 40°C Det.: FID set @ 220°C

1 2

3

4

5

6

7

8

910

min. 40

GC_FF00150

1-octen-3-ol and carvone - Rt-βDEXsa™

30m, 0.32mm ID, 0.25µm Rt-βDEXsa™ (cat.# 13108)Oven temp.: 40°C (hold 1 min.) to

230°C @ 2°C/min. (hold 3 min.) Carrier gas: hydrogenLinear velocity: 80cm/sec. set @ 40°C Det.: FID set @ 220°C

1

min. 20 30 40

21. (+/-)1-octen-3-ol2. (-)carvone3. (+)carvone

3

GC_FF00151

γ-lactones - Rt-βDEXcst™

1. (+/-)γ-heptalactone2. (+/-)γ-octalactone3. (+/-)γ-nonalactone4. (+/-)γ-decalactone5. (+/-)γ-dodecalactone

30m, 0.32mm ID, 0.25µm Rt-βDEXcst™

(cat.# 13102)Oven temp.: 60°C (hold 1 min.) to

200°C @ 1°C/min.Carrier gas: hydrogenLinear velocity: 40cm/sec. set @ 60°C Det.: FID set @ 220°C

min. 100 120 140

1

2

3

4

5

GC_FF00152

β-citronellol - Rt-βDEXsa™

30m, 0.32mm ID, 0.25µm Rt-βDEXsa™ (cat.# 13108)Oven temp.: 40°C (hold 1 min.) to 230°C

@ 2°C/min. (hold 3 min.) Carrier gas: hydrogenLinear velocity: 80cm/sec. set @ 40°CDet.: FID set @ 220°C

min. 51 52 53

1 2

1.(-)-(S)-β-citronellol2.(+)-(R)-β-citronellol

GC_FF00153

δ-lactones - Rt-δDEXcst™

1. (+/-)δ-pentalactone2. (+/-)δ-hexalactone3. (+/-)δ-heptalactone4. (+/-)δ-octalactone5. (+/-)δ-nonalactone6. (+/-)δ-decalactone7. (+/-)δ-dodecalactone

30m, 0.32mm ID, 0.25µm Rt-βDEXcst™ (cat.# 13102)Oven temp.:

60°C (hold 1 min.) to 200°C @ 1°C/min.

Carrier gas:hydrogen

Linear velocity: 40cm/sec. set @ 60°C

Det.:FID set @ 220°C

1

2

34

5

6

7

min. 100 120 140

GC_FF00154

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 626

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Apple Juice with Added Standards Rt-βDEXsm™

1. (R)-ethyl 2-methylbutyrate2. (S)-ethyl 2-methylbutyrate3. (R)-2-methylbutyrate4. (S)-2-methylbutyrate

30m, 0.32mm ID, 0.25µm Rt-βDEXsm™ (cat.# 13104)1.0µL split injectionOn-column conc. (standards): ~50ng each labeled enantiomer

Oven temp.: 40°C (hold 1 min.) to 220°C @ 2°C/min.Inj. & det. temp.: 220°CCarrier gas: hydrogenLinear velocity: 80cm/sec.

min. 4 6 8 10

1

2

4

3

GC_FF00155

Apple JuiceRt-βDEXsm™

2

3

min. 4 6 8 10GC_FF00156

Apple Juice with Added Standards Rt-βDEXse™

1

2

3

4

min. 8 10 12 14

30m, 0.32mm ID, 0.25µm Rt-βDEXse™ (cat.# 13106)1.0µL split injectionOn-column conc. (standards): ~50ng each labeled enantiomer

Oven temp.: 40°C (hold 1 min.) to 220°C @ 2°C/min.Inj. & det. temp.: 220°CCarrier gas: hydrogenLinear velocity: 80cm/sec.

1. (R)-ethyl 2-methylbutyrate2. (S)-ethyl 2-methylbutyrate3. (R)-2-methylbutyrate4. (S)-2-methylbutyrate

GC_FF00157

Apple JuiceRt-βDEXse™

2

3

min. 8 10 12 14

GC_FF00158

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 627

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628 Chiral Separations


Grape Juice ExtractRt-γDEXsa™

1. methyl (S)-(+)-3-hydroxybutyrate2. methyl (R)-(+)-3-hydroxybutyrate3. ethyl (S)-(+)-3-hydroxybutyrate4. ethyl (R)-(+)-3-hydroxybutyrate

30m, 0.32mm ID, 0.25µm Rt-γDEXsa™ (cat.# 13112)1.0µL split injectionOn-column conc.: ~150ng/enantiomer

Oven temp.: 40°C (hold 1 min.) to 200°C @ 2°C/min.Inj./det. temp.: 220°C/230°CCarrier gas: hydrogenLinear velocity: 80cm/sec. set @ 40°CSplit ratio: 25:1

min. 20 22 24 26 28

1

2

4

3

GC_FF00159

free literatureffooooddss,, ffllaavvoorrss,, && ffrraaggrraanncceess((lliitt.. ccaatt..## 5599226600AA))

A brief summary of many applications and chromatography products

This guide is a good overview of the foods - flavors - fragrances applications for which Restek GC andHPLC columns have been used. Application areas include amino acids, carbohydrates, chiral separations,essential oils, fats and oils, flavors and fragrances, organic acids, preservatives, and vitamins. Includes ahandy index of retention times for many flavor and fragrance compounds on a dimethylpolysiloxane or aPEG-type capillary GC column.

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Resolve critical enantiomer pairs quickly and reliably

In some analyses, particularly with pharmaceutical or flavor and fragrance compounds, enantiomer pairsmust be resolved for separate quantification. Five cyclodextrin-based Restek chiral capillary GC columnsoffer a unique range of selectivities, to enable you to accomplish these often-difficult separations. Manyexample chromatograms in this 24-page guide will help you find the best column - or columns - for yourparticular need.


www.restek.com

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 628

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Raspberry Flavor - Rt-βDEXsa™

30m, 0.32mm ID, 0.25µm Rt-βDEXsa (cat.# 13108) Oven temp.: 60°C (hold 2 min.) to 200°C @ 3°C/min. Carrier gas: helium; 60cm/sec. set @ 60°CDet.: MS set @ 220°C.

min. 10 20 30 40

1 2

1. (R)-α-ionone2. (S)-α-ionone

GC_FF00161

Peach/Vanilla Flavor - Rt-βDEXsa™

30m, 0.32mm ID, 0.25µm Rt-βDEXsa™ (cat.# 13108)Oven temp.: 60°C (hold 2 min.) to 100°C @ 15°C/min.

to 220°C @ 3°C/min.Carrier gas: helium, 60cm/sec. set @ 60°CDet.: MS set @ 220°C

min. 0 19 38

1. (R)-γ-octalactone2. (S)-γ-octalactone3. (R)-γ-decalactone4. (R)-γ-undecalactone5. (S)-γ-undecalactone

1 23

4 5

GC_FF00162

Bergamot Flavor - Rt-βDEXse™

30m, 0.32mm ID, 0.25µm Rt-βDEXse™ (cat.# 13106) Oven temp.: 40°C (hold 1 min.) to 200°C @ 4°C/min. Carrier gas: helium, 60cm/sec. set @ 40°CDet.: MS set @ 220°C

1. (S)-limonene 2. (R)-limonene 3. (R)-linalool 4. (S)-linalool 5. (R)-linalyl acetate 6. (S)-linalyl acetate

min. 8 13 18

1

2

34

5 6

GC_FF00163

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630 Fatty Acids


1. acetic acid2. propionic acid3. isobutyric acid4. n-butyric acid5. isovaleric acid6. n-valeric acid

8 10 12 14 16 18

2

1

3

4

5

6

30m, 0.32mm ID, 10µm Rt-QPLOT™ (cat.# 19718)Sample: fatty acids mixConc.: 5% of each compound in waterSample size: 0.5µLInlet liner: 3mm split w/glass wool (cat.# 20936-202.1)

Oven temp.: 75°C to 240°C @ 10°C/min. (hold 15 min.)Injector: split @ 240°CCarrier gas: hydrogen (constant pressure mode)Head pressure: 12.0psiColumn flow rate: 1.1cc/min. @ 100°CSplit ratio: 100:1Detector: FID @ 240°CMake-up gas flow: 40cc/min.

GC_FF00496

Fatty Acids (Free)Rt-QPLOT™

Fatty Acids (Free)Rtx®-200

1

2

34

5

67 8

9

min. 1 2 3 4GC_CH00282

30m, 0.25mm ID, 0.25µm Rtx®-200 (cat.# 15023)Sample: 0.8µL split injection of a free fatty acid standard.Conc.: approximately 10 to 20ng/µL.Oven temp.: 90°CInj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 1.4cc/min.)FID sensitivity: 4 x 10-11 AFSSplit vent: 40cc/min.

1. acetic acid2. propionic acid3. isobutyric acid4. n-butyric acid5. isovaleric acid6. n-valeric acid7. isocaproic acid8. caproic acid9. heptanoic acid

Fatty Acids (Free)Stabilwax®-DA

30m, 0.25mm ID, 0.25µm Stabilwax®-DA (cat.# 11023)1.0µL split injection of a free acid standard.Concentration approximately 10 to 20ng/µL.

Oven temp.: 145°CInj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec.FID sensitivity: 2 x 10-11 AFSSplit ratio: 50:1

GC_CH00280

min. 4 8 12

9

87

6

5

4

1

3

2


Fatty Acids (Free)Rtx®-1

GC_CH00281min. 4 8

1

2

3 4

56

7 8 9

30m, 0.53mm ID, 5.0µm Rtx®-1 (cat.# 10179)0.2µL injection of a 10–20ng/µL free fatty acid standard in water.Direct injection using a Uniliner® inlet liner.

Oven temp.: 60°C to 180°C @ 15°C/min.Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 50cm/sec. (flow rate: 6cc/min.)FID sensitivity: 4 x 10-11 AFS


624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 630

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S631Fatty Acids


Fatty Acids (Free) Stabilwax®-DA

GC_FF00653

Stabilwax®-DA 30m, 0.32mm ID, 0.25µm (cat.# 11024)Sample: 1.0µL FFA MixSolvent: waterConc.: 5mg/mL in methanolInj.: splitless/250°CSplitless hold time: 0.25min.Carrier gas: hydrogen (constant flow mode)Flow rate: 6.0mL/min. Split flow: 75mL/min.Det.: FID/250°CInlet liner: laminar cup splitter Oven temp.: 40°C to 250°C @ 10°C/min. (hold 15 min.)

1. C4:0 - butyric acid2. C6:0 - caproic acid3. C8:0 - caprylic acid4. C10:0 - capric acid5. C12:0 - lauric acid6. C14:0 - myristic acid7. C16:0 - palmitic acid8. C18:0 - stearic acid9. C18:1 - oleic acid

10. C18:2 - linoleic acid11. C18:3 - linolenic acid12. C20:0 - arachidic acid13. C22:0 - behenic acid

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632 FAMEs


GC_FF00649

FAMEs (AOAC 996.06 Standard)Rt-2560

Rt-2560, 100m, 0.25mm ID, 0.2µm (cat.# 13199)Sample: Food Industry FAME Mix (cat.# 35077),

30mg/mL total FAMEs in methylene chloride Inj.: 2.0µL split (split ratio 200:1), 4mm inlet liner (cat.# 20814)Inj. temp.: 225°CCarrier gas: hydrogen, constant flow Flow rate: 1.2mL/min.Oven temp.: 100°C (4 min. hold)

to 240°C @ 3°C/min. (10 min. hold) Det.: FID @ 250°C

CCoommppoouunndd %% iinn MMiixx1. C4:0 methyl butyrate 4.02. C6:0 methyl hexanoate 4.03. C8:0 methyl octanoate 4.04. C10:0 methyl decanoate 4.05. C11:0 methyl undecanoate 2.06. C12:0 methyl laurate 4.07. C13:0 methyl tridecanoate 2.08. C14:0 methyl myristate 4.09. C14:1 methyl myristoleate (cis-9) 2.0

10. C15:0 methyl pentadecanoate 2.011. C15:1 methyl pentadecenoate (cis-10) 2.012. C16:0 methyl palmitate 6.013. C16:1 methyl palmitoleate (cis-9) 2.014. C17:0 methyl heptadecanoate 2.015. C17:1 methyl heptadecenoate (cis-10) 2.016. C18:0 methyl stearate 4.017. C18:1 methyl elaidate (trans-9) 2.018. C18:1 methyl oleate (cis-9) 4.019. C18:2 methyl linoleaidate (trans-9,12) 2.020. C18:2 methyl linoleate (cis-9,12) 2.021. C20:0 methyl arachidate 4.022. C18:3 methyl γ-linolenate (cis-6,9,12) 2.023. C20:1 methyl eicosenoate (cis-11) 2.024. C18:3 methyl linolenate (cis-9,12,15) 2.025. C21:0 methyl heneicosanoate 2.026. C20:2 methyl eicosadienoate (cis-11,14) 2.027. C22:0 methyl behenate 4.028. C20:3 methyl eicosatrienoate (cis-8,11,14) 2.029. C22:1 methyl erucate (cis-13) 2.030. C20:3 methyl eicosatrienoate (cis-11,14,17) 2.031. C20:4 methyl arachidonate (cis-5,8,11,14) 2.032. C23:0 methyl tricosanoate 2.033. C22:2 methyl docosadienoate (cis-13,16) 2.034. C24:0 methyl lignocerate 4.035. C20:5 methyl eicosapentaenoate (cis-5,8,11,14,17) 2.036. C24:1 methyl nervonate (cis-15) 2.037. C22:6 methyl docosahexaenoate (cis-4,7,10,13,16,19) 2.0

Food Industry FAME Mixcat. # 35077

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 632

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S633FAMEs


FAMEs (NLEA Mix)Rt-2560

Column: Rt-2560 100m, 0.25mm ID, 0.20µm (cat.# 13199)Sample: NLEA FAME Mix (cat.# 35078), 30mg/mL

total FAMEs in methylene chloride Inj.: 1.0µL split (split ratio 100:1),

4mm inlet liner (cat.# 20814)Inj. temp.: 225°CCarrier gas: hydrogen, constant flow Flow rate: 1.2 mL/min.Oven temp.: 100°C (4 min. hold) to 240°C

@ 3°C/min. (10 min. hold) Det.: FID @ 250°C

1. C4:0 methyl butyrate2. C6:0 methyl hexanoate3. C8:0 methyl octanoate4. C10:0 methyl decanoate5. C11:0 methyl undecanoate6. C12:0 methyl laurate7. C13:0 methyl tridecanoate8. C14:0 methyl myristate9. C14:1 methyl myristoleate (cis-9)

10. C15:0 methyl pentadecanoate

11. C16:0 methyl palmitate12. C16:1 methyl palmitoleate (cis-9)13. C17:0 methyl heptadecanoate14. C18:0 methyl stearate15. C18:1 methyl elaidate (trans-9)16. C18:1 methyl oleate (cis-9)17. C18:2 methyl linoelaidate (trans-9,12)18. C18:2 methyl linoleate (cis-9,12)19. C20:0 methyl arachidate20. C20:1 methyl eicosenoate (cis-11)

21. C18:3 methyl linolenate (cis-9,12,15)22. C22:0 methyl behenate23. C22:1 methyl erucate (cis-13)24. C23:0 methyl tricosanoate25. C24:0 methyl lignocerate26. C20:5 methyl eicosapentaenoate (cis-5,8,11,14,17)27. C24:1 methyl nervonate (cis-15)28. C22:6 methyl docosahexaenoate (cis-4,7,10,13,16,19)

GC_FF00651

NLEA FAME Mixcat.# 35078

The Rt-2560 column providesthe resolution of cis & transFAMEs needed for AOACMethod 996.06

did you know?

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 633

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634 FAMEs


5,6

FAMEs (cis/trans isomers) Rtx®-Wax

60m, 0.25mm ID, 0.25µm Rtx®-Wax (cat.#12426)On-column conc.: 40–75ng.

Oven temp.: 165°C to 250°C @ 2°C/min. Inj./det. temp.: 220°C/250°CCarrier gas: heliumLinear velocity: 20cm/sec. set @ 165°CSplit ratio: 50:1

1

3

2

4

7

8

9

10

12

11

13

14

1. C14:02. C14:1n5 cis3. C14:1n5 trans4. C16:05. C16:1n7 cis6. C16:1n7 trans7. C18:08. C18:1 cis isomers

(n12, n9, n7)

9. C18:1 trans isomers(n12, n9, n7)

10. C18:2n6 cis11. C18:2n6 trans12. C20:013. C20:1n9 cis14. C20:1n9 trans

min. 15 20 25 30 35GC_FF00121

GC_FF00652

FAMEs (cis/trans isomers)Rt-2560

Rt-2560, 100m, 0.25mm ID, 0.2µm (cat.# 13199)Sample: cis/trans FAME Mix (cat.# 35079), 10mg/mL total FAMEs in methylene chloride Inj.: 1.0µL split (split ratio 20:1), 4mm inlet liner (cat.# 20814)Inj. temp.: 225°CCarrier gas: hydrogen, constant flow Flow rate: 1.2mL/min.Oven temp.: 100°C (4 min. hold)

to 240°C @ 3°C/min. (10 min. hold) Det.: FID @ 250°C

CCoommppoouunndd %% iinn MMiixx1. C18:0 methyl stearate 20.02. C18:1 methyl petroselaidate (trans-6) 8.03. C18:1 methyl elaidate (trans-9) 10.04. C18:1 methyl transvaccenate (trans-11) 12.05. C18:1 methyl petroselinate (cis-6) 8.06. C18:1 methyl oleate (cis-9) 10.07. C18:1 methyl vaccenate (cis-11) 12.08. C18:2 methyl linoleate (cis-9,12) 20.0

cis/trans FAME Mixcat. # 35079

excellent resolution ofcis & trans isomers

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S635FAMEs


FAMEs (Commercial Margarine) Rtx®-2330

1. C16:02. C18:03. C18:1 trans4. C18:1 trans5. C18:1 trans6. C18:1 trans7. C18:1 cis8. C18:1 cis9. C18:1 cis

10. C18:1 cis11. C18:212. C20:0

min. 4 8 12 16 20 24 28 32 36 40 44 48

1

2

3

5

4

6

8

9

10

11

7

12

105m, 0.25mm ID, 0.20µm Rtx®-2330 (cat.# 10729)1.0µL split injection of a commercial margarine mixture.Conc.: approximately 5µg.

Oven temp.: 165°CInj. & det. temp.: 275°CCarrier gas: hydrogenLinear velocity: 40cm/sec. FID sensitivity: 4 x 10-11 AFSSplit ratio: 50:1

GC_FF00123

FAMEs (Cocoa Butter) Stabilwax®

30m, 0.25mm ID, 0.25µm Stabilwax® (cat.# 10623)1.0µL split injection of a FAME reference standard for cocoa butter.

Oven temp.: 200°C to 250°C @ 8°C/min. (hold 3 min.)Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 31.4cm/sec. set @ 200°CFID sensitivity: 8 x 10-11 AFSSplit ratio: 45:1

1. C14:02. C16:03. C16:1n74. C17:05. C18:06. C18:1n97. C18:2n68. C18:3n39. C20:0

10. C20:1n911. C22:0

GC_FF00120

1

3

2 6

7

5

9

10114

min. 2 4 6 8

8

GC_FF00538

30m, 0.25mm, 0.25µm Rtx®-Wax (cat.# 12423)1µL split injection of saw palmetto standardConc.: see peak list

Oven temp.: 120°C (hold 3 min.) to 220°C at 20°C/min. (hold 12 min.)

Inj./det. temp.: 250°C/300°CCarrier gas: heliumLinear velocity: 1mL/min. (34 cm/sec.)Split ratio: 100:1

FAMEs (Saw Palmetto)Rtx®-Wax PPeeaakk LLiisstt CCoonncc.. ((mmgg//mmLL))

1. methyl caproate (C6:0) 0.42. methyl caprylate (C8:0) 0.43. methyl nonanoate (C9:0) 2.04. methyl caprate (C10:0) 0.45. methyl laurate (C12:0) 5.06. methyl myristate (C14:0) 2.07. methyl palmitate (C16:0) 2.08. methyl palmitoleate (C16:1) 0.49. methyl stearate (C18:0) 0.4

10. methyl oleate (C18:1) 5.011. methyl linoleate (C18:2) 1.012. methyl linolenate (C18:3) 0.4

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 635

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636 FAMEs


FAMEs (Marine Oil Standard)FAMEWAX™

GC_FF00568

1. C14:02. C15:03. C16:04. C16:15. C16:26. C17:07. C17:18. C16:49. C18:0

10. C18:1 (oleate)11. C18:1 (vaccenate)12. C18:2n6cis13. C18:3n314. C18:4n6

15. C18:4n316. C20:017. C20:1n718. C20:1n919. C20:4n620. C20:4n321. C20:5n322. C22:1n723. C21:5n324. C23:0 (IS)25. C22:5n626. C22:5n327. C22:6n328. C24:1

FAMEWAX™ 30m, 0.32mm ID, 0.25µm (cat.# 12498)Sample: 12mg/mL total FAMEsInj.: 0.5µL, split (150:1), 3mm ID split liner for Trace Series GCs, packed

with glass wool (cat.# 20936-202.1)Inj. temp.: 250°CCarrier gas: hydrogen, constant flowLinear velocity: 62cm/sec.Oven temp.: 195°C to 240°C @ 5°C/min. (hold 1 min.)Det.: FID, 250°C

FAST analysis ofmarine oil FAMEs

PPeeaakk LLiisstt CCoonncc.. ((µgg//mmLL))1. C14:0 6002. C14:1 1003. C16:0 16004. C16:1 5005. C18:0 8006. C18:1 (oleate) 13007. C18:1 (vaccenate) 4008. C18:2 2009. C18:3 200

10. C20:0 10011. C20:1 90012. C20:2 10013. C20:4 30014. C20:3 10015. C20:5 100016. C22:0 10017. C22:1 30018. C24:0 10019. C22:6 120020. C24:1 100

FAMEs (Marine Oil Standard)FAMEWAX™

30m, 0.32mm ID, 0.25µm FAMEWAX (cat. # 12498)Inj.: 1µLConc.: 10,000µg/mL in isooctane (total FAMES; see breakdown in peak list)

Oven temp.: 195–240°C at 5°C/min., 1 min. holdInj. / det. temp.: 250°C / 275°CCarrier gas: hydrogenFlow rate: 3 mL/min. (constant flow)Split ratio: 100:1

GC_FF00499

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S637FAMEs


87

13

FAMEs (PUFA, marine source)Rtx®-2330

30m, 0.25mm ID, 0.20µm Rtx®-2330 (cat.# 10723)0.5µL split injection of a PUFA mix.

Oven temp.: 160°C to 225°C @ 2°C/min. Inj. & det. temp.: 225°C/250°CCarrier gas: hydrogenLinear velocity: 45cm/sec. set @ 160°CFID sensitivity: 8 x 10-11 AFSSplit ratio: 35:1

1

2

3 4

65

9

11

10

12

14

15

1617

18

min. 5 10 15 20GC_FF00118

1. C14:02. C16:03. C16:1n74. C18:1n95. C18:1n76. C18:2n67. C18:3n38. C20:1n119. C20:1n9

10. C20:1n7 11. C18:4n3 12. C22:1n11 13. C22:1n9 14. C20:4n3 15. C20:5n316. C21:5n3 17. C22:5n3 18. C22:6n3

5

FAMEs (PUFA, animal source)Rtx®-2330

1. C14:02. C16:03. C16:1n74. C18:05. C18:1n96. C18:1n77. C18:2n68. C18:3n69. C18:3n3

10. C20:3n611. C20:4n612. C20:5n313. C22:4n614. C22:5n315. C22:6n3

30m, 0.32mm ID, 0.20µm Rtx®-2330 (cat.# 10724)0.1µL split injection of PUFA 2 mix.


Inj. & det. temp.: 260°CCarrier gas: hydrogenLinear velocity: 40cm/sec. FID sensitivity: 8 x 10-11 AFSSplit ratio: 20:1

min. 4 8 12 16 20 24 28

1

2

3

4

6

7

8

9

10

11

12

13

14

15

GC_FF00119

12

FAMEs (PUFA, animal source)MXT®-WAX

min. 4 8 12 16 20 24 28 32

1

2

3

4 5

6

7

8

9

10 11

13

14

15

16

17

GC_FF00117

18

1. C14:02. C16:03. C16:1n74. C18:05. C18:1n96. C18:1n77. C18:2n68. C18:3n39. C18:4n3

10. C20:1n911. C20:2n612. C20:3n613. C20:4n614. C20:5n315. C22:4n616. C22:5n317. C22:6n318. C24:1n9

30m, 0.28mm ID, 0.25µm MXT®-WAX (cat.# 70624)0.1µL split injection of PUFA 2 mix.

Oven temp.: 160°C to 250°C @ 2°C/min. (hold 10 min.)Inj. & det. temp.: 260°CCarrier gas: hydrogenLinear velocity: 40cm/sec. FID sensitivity: 8 x 10-11 AFSSplit ratio: 20:1

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 637

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638 FAMEs


FAMEs (Evening Primrose Oil)FAMEWAX™

1

2

3

4

5

min. 20 40

1. C16:02. C18:03. C18:1n94. C18:2n65. C18:3n6

GC_FF00363

FAMEs (Flax Seed Oil)FAMEWAX™

min. 20 40

1

2

3

4

61. C16:02. C18:03. C18:1n94. C18:2n65. C18:3n66. C18:3n3

GC_FF00364

FAMEs (Black Currant Seed Oil)FAMEWAX™

min. 20 40

1

2

3

4

5

6

GC_FF00365

1. C16:02. C18:03. C18:1n94. C18:2n65. C18:3n66. C18:3n3

FAMEs (Borage Seed Oil)FAMEWAX™

CCoolluummnn aanndd ccoonnddiittiioonnss ffoorr aallll ffoouurr ooiillss aannaallyysseess::30m, 0.25mm ID, 0.25µm FAMEWAX™ (cat.# 12497).Oven temp.: 165°C (hold 30 min.) to 220°C @

1.5°C/min. (hold 15 min.) Inj. temp.: 225°CDet. temp.: 230°CCarrier gas: helium @ 40cm/sec.

min. 20 40

1

2

3

4

5

GC_FF00366

1. C16:02. C18:03. C18:1n94. C18:2n65. C18:3n6

624-638_GCAPP.qxd 11/4/2004 8:48 AM Page 638

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S639Essential Oils


Spearmint Oil (Native )Stabilwax®

1 2

3

4

5

6 7

8

912

1011

13

14

15

16

17

18

19

20

21

22

23

24

26 27

28

29

30

25

GC_FF00140

1. α-pinene2. β-pinene3. sabinene4. myrcene5. α-terpinene6. l-limonene7. 1,8-cineole8. cis-ocimene9. γ-terpinene

10. p-cymene

11. terpinolene12. 3-octyl acetate13. 3-octanol14. l-menthone15. trans-sabinenehydrate16. β-bourbonene17. linalool18. terpinene-4-ol19. β-caryophyllene20. dihydrocarvone

21. trans-dihydrocarvyl acetate22. trans-β-farnesene23. α-terpineol24. germacrene-∆25. carvone26. cis-carvyl acetate27. trans-carveol28. cis-carveol29. cis-jasmone30. viridiflorol

60m, 0.25mm ID, 0.25µm Stabilwax® (cat.# 10626)0.2µL split injection of a neat spearmint oil


Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. set @ 160°C FID sensitivity: 4 x 10-11 AFSSplit ratio: 100:1

min. 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32

2 3 5

19

Lemon OilRtx®-5

GC_FF00138

min. 2 4 6 8 10 12 14 16 18 20 22 24 26 28

1

4

6

9

7,8

1110

12

13

1415

16

17

18

2021 22

2324

25

26

27,28

29

3031

32

33

34

35

36

38

37

1. heptanol2. α-thujene3. α-pinene4. camphene5. sabinene6. β-pinene7. 6-methyl-5-hepten-2-one8. myrcene9. octanal

10. α-phellandrene11. 3-carene12. α-terpinene13. p-cymene14. limonene15. γ-terpinene16. octanol17. terpinolene18. linalool19. nonanal20. citronellal21. terpinene-4-ol22. α-terpineol23. decanol24. octyl acetate25. nerol26. neral27. carvone28. geraniol29. geranial30. nonyl acetate31. citronellyl acetate32. neryl acetate33. geranyl acetate34. dodecanal35. β-caryophyllene36. trans-α-bergamotene37. α-humulene38. β-bisabolene

30m, 0.32mm ID, 0.25µm Rtx®-5 (cat.# 10224)Wet needle split injection of a neat lemon oil

Oven temp.: 75°C (hold 8 min.) to 250°C @ 4°C/min.Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 3.2cc/min.) FID sensitivity: 2 x 10-11 AFSSplit ratio: 100:1

639-662_GCAPP.qxd 11/4/2004 2:01 PM Page 639

Peppermint OilMXT®-WAX

1

2

3

4

5

6

7

8

9

1011

12

13

14,15

16

17

18 19

20

21

22

23

24

2526

27

28

29

min. 4 8 12 16 20 24 28 32 36

GC_FF00141

1. α-pinene2. β-pinene3. sabinene4. myrcene5. α-terpinene6. l-limonene7. 1,8-cineole8. cis-ocimene9. γ-terpinene

10. p-cymene11. terpinolene12. 3-octanol13. 1-octen-3-ol14. l-menthone15. trans-sabinenehydrate

16. menthofuran17. d-isomenthone18. β-bourbonene19. linalool20. menthyl acetate21. neo-menthol22. β-caryophyllene23. terpinene-4-ol24. l-menthol25. pulegone26. α-terpineol27. germacrene-∆28. piperitone29. viridiflorol

30m, 0.28mm ID, 0.50µm MXT®-WAX (cat.# 70639)1.0µL split injection of peppermint oil

Oven temp.: 75°C (hold 4 min.) to 240°C @ 4°C/min.Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. set @ 75°CFID sensitivity: 16 x 10-11 AFSSplit ratio: 50:1

Inert stainless steel equivalentto Stabilwax® fused silicacolumns!

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640 Essential Oils


free literatureffooooddss,, ffllaavvoorrss,, && ffrraaggrraanncceess((lliitt.. ccaatt..## 5599226600AA))

A brief summary of many applications and chromatography products

This guide is a good overview of the foods - flavors - fragrances applications for which RestekGC and HPLC columns have been used. Application areas include amino acids, carbohydrates,chiral separations, essential oils, fats and oils, flavors and fragrances, organic acids, preserva-tives, and vitamins. Includes a handy index of retention times for many flavor and fragrancecompounds on a dimethylpolysiloxane or a PEG-type capillary GC column.

AA GGuuiiddee ttoo tthhee AAnnaallyyssiiss ooff CChhiirraall CCoommppoouunnddss bbyy GGCC((lliitt.. ccaatt..## 5599888899))

Resolve critical enantiomer pairs quickly and reliably

In some analyses, particularly with pharmaceutical or flavor and fragrance compounds, enan-tiomer pairs must be resolved for separate quantification. Five cyclodextrin-based Restek chiralcapillary GC columns offer a unique range of selectivities, to enable you to accomplish theseoften-difficult separations. Many example chromatograms in this 24-page guide will help youfind the best column - or columns - for your particular need.

639-662_GCAPP.qxd 11/4/2004 2:01 PM Page 640

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S641Essential Oils


Synthetic Mushroom AromaRtx®-20

30m, 0.32mm ID, 1.0µm Rtx®-20 (cat.# 10354)1.0µL split injection of a synthetic mushroom aroma.Conc.: 10ng per component.

Oven temp.: 45°C (hold 8 min.) to 250°C @ 4°C/min.Inj. & det. temp.: 260°CCarrier gas: hydrogenLinear velocity: 40cm/sec.FID sensitivity: 4 x 10-11 AFSSplit ratio: 100:1

1. acetone2. ethyl acetate3. 1-butanol4. 3-methyl-1-butanol5. 1-pentanol6. hexanal7. furfural8. amyl acetate9. 1-octen-3-ol

10. 3-octanol11. 3-octanone12. benzaldehyde13. octyl alcohol14. benzyl alcohol15. phenylacetaldehyde16. nonanal17. α-terpineol18. 2,4-nonadienal19. 2,4-decadienal

GC_FF00142

min. 4 8 12 16 20 24 28 32 36

1

2

3

4

5

6

7

8

109

11

12

13

14

15

16

17

1819

Synthetic Mushroom AromaStabilwax®

GC_FF00143

1

2

3

45

6

7 8

9

10

11

12

13

14

15

16

17

1819

20

min. 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30

1. acetone2. ethyl acetate3. methylene chloride (solvent)4. hexanal5. amyl acetate6. 1-butanol7. 3-methyl-1-butanol8. 1-pentanol9. 3-octanone

10. 3-octanol11. nonanal12. 1-octen-3-ol13. furfural14. benzaldehyde15. octyl alcohol16. phenylacetaldehyde17. α-terpineol18. 2,4-nonadienal19. 2,4-decadienal20. benzyl alcohol

30m, 0.32mm ID, 1.0µm Stabilwax® (cat.# 10654)1.0µL split injection of a synthetic mushroom aromaConc.: 10ng per component

Oven temp.: 40°C to 220°C @ 6°C/min.Inj. & det. temp.: 260°CCarrier gas: hydrogenLinear velocity: 40cm/sec. FID sensitivity: 4 x 10-11 AFSSplit ratio: 100:1

639-662_GCAPP.qxd 11/4/2004 2:01 PM Page 641

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642 Essential Oils


min. 6 8 10 12 14 16 18 20 22 24 26 28

1

3

8

13

4

16

912

6

5

117

15

14102

Citronella Java OilRtx®-1

60m, 0.25mm ID, 0.25µm Rtx®-1 (cat.# 10126)1.0µL split injection of citronella Java oil

Oven temp.: 100°C to 260°C @ 4°C/min. (hold 1 min.)Inj. / det. temp.: 250°C / 280°CDet. type: MSDCarrier gas: heliumLinear velocity: 30cm/sec. set @ 50°CFID sensitivity: 2 x 10-11 AFSSplit vent: 100cc/min.

GC_FF00144

Citronella Java OilStabilwax®

60m, 0.25mm ID, 0.25µm Stabilwax® (cat.# 10626)1.0µL split injection of citronella Java oil

Oven temp.: 100°C to 260°C @ 4°C/min. (hold 1 min.)Inj. / det. temp.: 250°C / 280°CDet. type: MSDCarrier gas: heliumLinear velocity: 30cm/sec. set @ 50°CFID sensitivity: 2 x 10-11 AFSSplit vent: 100cc/min.

min. 5 10 15 20 25

15

16

9

12

13

8

7

6

5

4

3

1 10,11 142

GC_FF00145

1. limonene2. citronellal3. linalool4. borneol5. γ-elemene6. β-caryophyllene7. neryl acetate8. α-terpineol9. germancrene-∆

10. β-citronellol11. geranyl acetate12. δ-cadenene13. nerol14. geraniol15. eugenol16. α-bergamotene

639-662_GCAPP.qxd 11/4/2004 2:01 PM Page 642

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S643


Essential Oils

Flavor VolatilesRtx®-1

1. methanol2. acetaldehyde3. ethanol4. acetone5. isopropyl alcohol6. methylene chloride7. hexane8. ethyl acetate9. ethyl propionate

10. n-hexanal

11. ethylbutyrate12. furfural13. trans-2-hexenal14. α-thujene15. α-pinene16. camphene17. sabinene18. β-pinene19. octanal20. myrcene

21. α-phellandrene22. α-terpinene23. p-cymene24. δ-limonene25. γ-terpinene26. octanol27. terpinolene28. nonanal29. linalool30. cis-limonene monoxide

31. trans-limonene monoxide32. citronellal33. terpinene-4-ol34. α-terpineol35. decanal36. d & l carveol37. neral38. carvone39. geranial40. neryl acetate

41. geranyl acetate42. α-ionone43. β-caryophyllene44. trans-α-bergamotene45. BHA46. β-ionone47. valencene48. γ-elemene49. β-bisabolene50. nootketone

60m, 0.53mm ID, 0.50µm Rtx®-1 (cat.# 10143)0.8µL split injection of a flavor volatiles test mix

Oven temp.: 70° (hold 15 min.) to 190°C @ 2°C/min. (hold 5 min.)Inj. / det. temp.: 220°C/260°CCarrier gas: heliumLinear velocity: 20cm/sec. set @ 70°CFID sensitivity: 64 x 10-11 AFSSplit ratio: 20:1

50

49

47

48

4645

44

43

4241

40

39

38

37

36

35

34

33

32

3130

28,29

27262524

23

22

21

19,201817

16

151413121011

9

7,8

6

1,23 4,5

min. 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80GC_FF00536

min. 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72

1. hexane2. acetaldehyde3. acetone4. methanol5. ethyl acetate6. isopropyl alcohol7. ethanol8. methylene chloride9. ethyl propionate

10. α-pinene11. ethylbutyrate12. n-hexanal13. β-pinene14. sabinene15. myrcene16. δ-limonene17. 1,8-cineole18. trans-2-hexenal

19. γ-terpinene20. p-cymene21. terpinolene22. octanal23. nonanal24. cis-limonene monoxide25. trans-limonene26. furfural27. citronellal

28. decanal29. linalool30. octanol31. trans-α-bergamotene32. β-caryophyllene33. terpinene-4-ol34. neral35. α-terpineol36. neryl acetate

37. valencene38. geranial39. carvone40. geranyl acetate41. d/l carveol42. α-ionone43. d/l carveol44. β-ionone

60m, 053mm ID, 0.50µm Stabilwax® (cat.# 10643)0.8µL split injection of a flavor volatiles test mix

Oven temp.: 70°C (hold 15 min.) to 190°C @ 2°C/min. (hold 5 min.)Inj. / det. temp.: 220°C/260°CCarrier gas: heliumLinear velocity: 20cm/sec. set @ 70°CFID sensitivity: 64 x 10-11 AFSSplit ratio: 20:1

Flavor VolatilesStabilwax®

4443

424140

38

3937

36

35

34

33

32

31

30

29

28

27

26

2524

23

22

2120

19

18

1716151413

121110

9

7,84,5

3

21

6

GC_FF00537

639-662_GCAPP.qxd 11/4/2004 2:01 PM Page 643

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644 Flavors & Fragrances


Malt WhiskeyStabilwax®-DA

Stabilwax®-DA 30m, 0.18mm ID, 0.18µm (cat.# 550752)

Inj.: 10µL large volume injection (splitless),at 10µL/min.

Std. conc.: neatGerstel CIS Injector: 35°C (hold 2 min.), to 300°C @

10°C/sec. (hold 5 min.)Helium vent flow: 600mL/min. with 1.8 min. vent end timeCarrier gas: heliumLinear velocity: 45cm/sec.Oven temp.: 60°C (hold 2 min.) to 100°C @

20°C/min., to 240°C @ 5°C/min.(hold 10 min.)

Det.: MSDTransfer line temp.: 240°CQuadrupole temp.: 150°CMS source temp.: 230°CScan range: 30–400amuIonization: 70eVMode: EI

Chromatogram courtesy of Kevin MacNamara, Ph.D., Irish Distilleries, Ltd.

1. ethyl octanoate2. acetic acid3. siloxane4. 1-hydroxy-2,3-butadione5. 1-hydroxy-2-propanone acetate6. furfural7. formic acid8. propionic acid9. isobutyric acid

10. dimethyl sulfoxide11. 5-methyl furfural12. methyl decanoate + unknown13. butyric acid14. siloxane15. ethyl decanoate16. furfuryl alcohol17. isoamyl octanoate18. isovaleric acid19. 2-methylbutyric acid20. diethyl succinate21. 3-methyl-2(5H)-furanone (possible)22. valeric acid

23. ethyl undecanoate24. isobutyl decanoate25. 2(5H)-furanone26. unknown27. di(ethyleneglycol) butyl ether28. siloxane29. methyl dodecanoate30. 2-phenylethyl acetate31. methylcyclopentenolone32. hexanoic acid33. ethyl dodecanoate34. isoamyl decanoate35. guaiacol36. dodecyl acetate (possible)37. whiskey lactone (1)38. 2-phenylethanol39. heptanoic acid40. siloxane41. dimethoxybenzene or 4-methyl-

guaiacol42. whiskey lactone (2)43. dodecanol

44. unknown45. phenol46. methyl tetradecanoate47. nerolidol48. diethyl malate49. ethyl tetradecanoate50. octanoic acid51. unknown52. p-cresol53. siloxane54. diethyl octanedioate55. monomethyl succinate (possible)56. 3,5-dimethyl-2,4(5H)furandione57. nonanoic acid58. diethyl 2-hydroxyglutarate59 unknown60. tetradecanol61. 4-vinylguaiacol62. diethyl nonanedioate63. methyl hexadecanoate64. ethyl γ-lactone 2-hydroxyglutarate65. ethyl hexadecanoate

66. decanoic acid67. ethyl 9-hexadecanoate68. triacosan69. unknown70. phthalide71. diethyl phthalate72. hexadecanol73. 4-hydroxycinnamic acid (decomp.)74. methyl stearate75. benzoic acid76. methyl 8-octadecenoate77. ethyl stearate78. dodecanoic acid79. hydroxymethylfurfural80. ethyl linoleate81. 4-allyl-2,6-dimethoxyphenol82. diisobutyl phthalate83. vanillin84. sinapic acid (decomp.)85. 2-phenylethyl decanoate

+2 unknowns86. 4-propenyl-2,6-dimethoxyphenol

87. ethyl vanillate88. acetovanillone89. vanillin methyl ketone90. tetradecanoic acid91. ethyl homovanillate92. propiovanillone93. fatty acid ester94. (similar to 4-allyl-2,6-

dimethoxyphenol)95. unknown96. hexadecanoic acid97. syringealdehyde

GC_FF00603

639-662_GCAPP.qxd 11/4/2004 2:01 PM Page 644

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S645Flavors & Fragrances


RumRtx®-1301

1. acetaldehyde2. methanol3. ethanol4. acetone5. ethyl formate6. isopropanol7. n-propanol8. ethyl acetate

9. sec-butanol10. isobutanol11. isoamyl alcohol12. active amyl alcohol13. n-amyl alcohol

60m, 0.25mm ID, 1.4µm Rtx®-1301 (cat.# 16016)1.0µL split injection using a Cyclosplitter® liner (cat.# 20706).Conc.: neat

Oven temp.: 35°C (hold 5 min.) to 100°C @ 1°C/min.Inj./det. temp.: 150°C / 200°CCarrier gas: hydrogen @ 40cm/sec.Split ratio: 100:1

min. 5 10 15 20 25

1

2

4

5

6

7

8

3

9

1011

12

13

GC_FF00110

RumCarboBlack™ B

5% Carbowax® 20M 80/120 CarboBlack™ B (cat.# 80105)2m, 1/8" OD x 2mm ID SilcoSmooth™ tubing0.5µL on-column injection, on-column conc.: neat

Oven temp.: 65°C (hold 5 min.) to 150°C @4°C/min.

Inj./det. temp.: 200°C / 250°CDet.: FIDCarrier gas: nitrogenColumn flow rate: 20mL/min.FID sensitivity: 1.28 x 10-10 AFS

1

2

3

4

5

6

7

8

9

12

11

10

min. 10 20GC_FF00111

1. acetaldehyde2. methanol3. acetone4. ethyl formate5. ethanol6. ethyl acetate7. n-propanol8. sec-butanol9. isobutanol

10. active amyl alcohol11. isoamyl alcohol12. n-amyl alcohol

ScotchCarboBlack™ B

5% Carbowax® 20M 80/120 CarboBlack™ B (cat.# 80105)2m, 1/8" OD x 2mm ID Silcosmooth™ tubing0.5µL on-column injection, on-column conc.: neat

Oven temp.: 70°C to 150°C @ 4°C/min.Inj./det. temp.: 200°C / 250°CDet.: FIDCarrier gas: nitrogenColumn flow rate: 20mL/min.FID sensitivity: 1.28 x 10-10 AFS

1. acetaldehyde2. methanol3. ethanol4. ethyl acetate5. n-propanol6. isobutanol7. acetic acid8. active amyl alcohol9. isoamyl alcohol

12

4

5

6

9

8

7

min. 5 10 15

3

GC_FF00113

ScotchRtx®-1301

60m, 0.25mm ID, 1.4µm Rtx®-1301 (cat.# 16016)1.0µL split injection using a Cyclosplitter® sleeve (cat.# 20706).Conc.: neat

Oven temp.: 35°C (hold 5 min.) to 100°C @ 1°C/min.Inj./det. temp.: 150°C / 200°CCarrier gas: hydrogen @ 40cm/sec.Split ratio: 100:1

1

2

3

4

6

7

5

min. 5 10 15 20 25

8

9

10 11

1. acetaldehyde2. methanol3. ethanol4. acetone5. isopropanol6. n-propanol

7. ethyl acetate8. isobutanol9. acetic acid

10. isoamyl alcohol11. active amyl alcohol

aceticacid

GC_FF00112

639-662_GCAPP.qxd 11/4/2004 2:01 PM Page 645

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Alcoholic Beverage Standard: Acids and EstersStabilwax®-DA

PPeeaakk LLiisstt CCoonncc.. ((ppppmm))1. ethyl octanoate 1002. acetic acid 1003. propionic acid 1004. isobutyric acid 1005. 3-decanol 506. ethyl decanoate 507. ethyl laurate 508. cis-lactone 1009. 2-phenylethanol 50

10. trans-lactone 10011. methyl myristate 5012. ethyl myristate 5013. octanoic acid 10014. ethyl palmitate 5015. decanoic acid 10016. dodecanoic acid 10017. vanillin 100

Stabilwax®-DA 30m, 0.18mm ID, 0.18µm (cat.# 550752)Inj.: 1µL splitless (hold 0.5 min.) at conc. shown in

peak list, in ethyl acetate, 4mm ID splitless liner w/wool (cat.# 20814-202.1)

Inj. temp.: 240°CCarrier gas: hydrogenMake-up gas: nitrogenLinear velocity: 28psi @ 240°COven temp.: 70°C to 240°C at 12°C/min. (hold 3 min.)Det.: FID

GC_FF00500

free literatureAAnnaallyyzziinngg AAllccoohhoolliicc BBeevveerraaggeess bbyy GGaass CChhrroommaattooggrraapphhyy

((lliitt.. ccaatt..## 5599446622))

Selectivity, sensitivity, and minimal sample preparation make GC a powerful tool for monitoring alcoholicbeverage composition

Volatile component profiles of alcoholic beverages reveal a wide range of compounds: acids, alcohols, alde-hydes, and others. This 16-page guide describes packed column GC and capillary GC approaches to monitor-ing these complex mixtures of analytes. A separate section is devoted to detailed information about quanti-fying trace sulfur compounds in beer.

639-662_GCAPP.qxd 11/4/2004 2:01 PM Page 646

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S647Flavors & Fragrances


GC_FF00657

5% Fragrance Materials Association Mix Rtx®-1

Rtx®-1, 60m, 0.25mm ID, 0.25µm (cat.# 10126)Sample: 5% FMA mix in acetone (cat.# 31807) Inj.: 1.0µL split (split ratio 40:1),

4mm inlet liner (cat.# 20814)Inj. temp.: 285°CCarrier gas: helium, constant flow Flow rate: 0.6mL/min.Oven temp.: 50°C to 270°C @ 3°C/min. Det.: FID @ 300°C

CCoommppoouunndd %% iinn tthhee FFMMAA mmiixx1. ethyl butyrate 35.72. limonene 20.03. eucalyptol 0.54. benzoic acid 1.05. cinnamic aldehyde 0.56. geraniol 0.67. hydroxycitronellal 5.08. thymol 0.39. cinnamyl alcohol 0.3

10. vanillin 0.111. cinnamyl acetate 0.312. benzyl salicylate 35.7

FMA Reference Mixcat. # 31807

5% Fragrance Materials Association MixRtx®-1701

GC_FF00658

Rtx®-1701, 60m, 0.25mm ID, 0.25µm (cat.# 12026)Sample: 5% FMA mix in acetone Inj.: 1.0µL split (split ratio 40:1), 4mm inlet liner (cat.# 20814)Inj. temp.: 285°CCarrier gas: helium, constant flow Flow rate: 0.6mL/min.Oven temp.: 50°C to 270°C @ 3°C/min. Det.: FID @ 300°C

CCoommppoouunndd %% iinn FFMMAA MMiixx1. ethyl butyrate 35.72. limonene 20.03. eucalyptol 0.54. geraniol 0.65. benzoic acid 1.06. cinnamic aldehyde 0.57. hydroxycitronellal 5.08. thymol 0.39. cinnamyl alcohol 0.3

10. cinnamyl acetate 0.311. vanillin 0.112. benzyl salicylate 35.7

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50m, 0.32mm ID, 0.33µm, traditional Carbowax® 20M or Rt-CW20M™ F&F (cat.# 12539) On-column injection of 5ng to 150ng each compound in methylene chloride, split 10:1

Carrier gas: hydrogen, 40cm/sec.Inj. & det. temp.: 220°COven temp.: 110°C isothermal

Rt-CW20M™ F&F columnCarbowax® column

9 10

min. 5 10 15 20

1

23

4

6 7

5

12

11

13

14

15

12

3

4

5

12

13

14

8

8

9 106 7 11

15

1. methyl heptanoate2. hexanol3. methyl octanoate4. nonanal5. menthone6. citronellal7. methyl nonanoate8. 2,3-butanediol9. linalool

10. linalyl acetate11. methyl decanoate12. menthol13. α-terpineol14. γ-terpineol15. methyl undecanoate

better response for 2,3-butanediol

Flavor & Fragrance CompoundsRt-CW20M™ F&F

min. 5 10 15 20GC_FF00433

a plus one story“Thank you for being a top notch GC Analytical Supply Company that always continues to offernewer products & services.”

JJoohhnn TT.. BBrruunneettttee,, Chemist, Bausch & Lomb

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S649Food Contaminants


AcrylamideStabilwax®

Stabilwax® 15m, 0.53 ID, 0.50µm (cat.# 10637)Sample: 25µg/mL acrylamide standard in waterInj.: 1.0µL, 0.5min holdLiner: 2mm splitless with wool (cat.# 20830)Inj. temp.: 260°CCarrier gas: helium, constant pressureLinear velocity: 62cm/sec. @ 100°COven temp.: 100°C (hold 0.5 min.) to 200°C @ 15°C/min.Det.: FID @ 260°C

GC_FF00642

1. acrylamide

Acrylamide (Potato Chip Extract)Stabilwax®

GC_FF00643

Stabilwax® 15m, 0.53mm ID, 0.50µm (cat.# 10637)Sample: 1g of potato chips spiked with 100µg acrylamideInj.: 1.0µL, 0.5min holdLiner: 2mm splitless with wool (cat.# 20830)Inj. temp.: 260°CCarrier gas: helium, constant pressureLinear velocity: 62cm/sec. @ 100°COven temp.: 100°C (hold 0.5 min.) to 200°C @ 15°C/min.Det.: FID @ 260°C

1. acrylamide

blank

1m, 0.75mm ID Sulfinert® tubingRt-XLSulfur™ 100/120 mesh (cat.# 19806)Conc.: sulfur standard @ 20ppb each in CO2

Inj.: 1cc sample loop, 6-port Valco® valveCarrier gas: heliumFlow rate: 10mL/ min. @ ambient temp.Oven temp.: 60°C to 260°C @ 15°C/min. (hold 5 min.) Det. sensitivity: SCD, attn. x 1Det. temp.: 800°C

GC_FF00491

Sulfur Compounds in Beverage Grade CO2

Rt-XLSulfur™

1. hydrogen sulfide2. carbonyl sulfide3. methyl mercaptan4. ethyl mercaptan and/or

dimethyl sulfide5. dimethyl disulfide

GC_FF00494

1m, 0.75mm ID Sulfinert® tubingRt-XLSulfur™ 100/120 mesh (cat.# 19806)Conc.: headspace of a domestic (US) beer sampleInj.: 1cc sample loop, 6-port Valco® valveCarrier gas: heliumFlow rate: 10mL/min. @ ambient temp.Oven temp.: 60°C to 260°C @ 15°C/min. (hold 5 min.) Det. sensitivity: SCD, attn. x 1Det. temp.: 800°C

1. hydrogen sulfide2. methyl mercaptan3. dimethyl sulfide and/or

ethyl mercaptan4. unknown

Sulfur Compounds in Beer HeadspaceRt-XLSulfur™

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650 Food Contaminants


1

2

9

34

5

6 78 10

11

12

0 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21

VVoollaattiilleess SSttaannddaarrdd1. isopropanol2. methylene chloride3. 2-methyl-1-propanol4. tetrahydrofuran5. benzene6. toluene7. hexanal8. furfural9. 4-heptanone (IS)

10. butyl ether11. styrene12. dodecane

30m, 0.25 mm ID, 1µm Rtx®-5MS (cat.# 12653)

Concentrator: Tekmar LSC-3100 purge & trapTrap: Vocarb 3000, type KPurge: 10 min. @ 40mL/min. @ 60°CDry purge: 3 min. @ 40mL/min.Desorb preheat: 220°CDesorb: 245°C, 2 min., 40mL/min.Bake: 230°C, 6 min.Interface: 1:20 split at injection port with 1mm ID linerTransfer line: 0.53mm ID Silcosteel® MXT® tubing, cat.# 20591Oven temp.: 50°C to 92°C @ 3°C/min., to 220°C @

20°C/min., hold 1 min. Inj. temp.: 250°CCarrier gas: heliumLinear velocity: 36cm/sec (1mL/min.)Split ratio: 20:1

Det.: MSDDet. temp.: 280°CScan range: 35–260 AMUIonization: EI @ 70 eV

Food Packaging Volatiles (Volatiles Standard)Rtx®-5MS

GC_FF00457

1. tetrahydrofuran2. 1-butanol3. toluene4. hexanal5. ethylbenzene6. 4-heptanone7. butyl ether8. styrene9. 1-methylethylbenzene

10. propylbenzene11. benzaldehyde12. benzeneacetaldehyde13. acetophenone14. benzoic acid, methyl ester15. decanal

Rtx®-5MS 30m, 0.25 mm ID, 1µm (cat.# 12653)Concentrator: Tekmar LSC-3100 purge & trapTrap: Vocarb 3000, type KPurge: 10 min. @ 40 mL/min., @ 60°CDry purge: 3 min. @ 40mL/min.Desorb preheat: 220°CDesorb: 245°C, 2 min., 40mL/min.Bake: 230°C, 6 min.Interface: 1:20 split at injection with 1mm ID linerTransfer line: 0.53mm ID Silcosteel® MXT® tubing (cat.# 20591)Inj. temp.: 250°CCarrier gas: heliumLinear velocity: 36cm/sec. (1mL/min.)Split ratio: 20:1Oven temp.: 50°C to 92°C @ 3°C/min., to 220°C @

20°C/min, hold for 1 min.Detector : MSD (Agilent 5973)Det. temp.: 280°CScan range: 35-260amuIonization: EI @ 70 eV

GC_FF00459

Food Packaging Volatiles Rtx®-5MS

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S651Sterols


Sterols (Olive Oil)XTI®-5

30m, 0.25mm ID, 0.25µm XTI®-5 (cat.# 12223)0.5µL splitless injection of a sterol fraction from olive pomace oilConc.: 10–2500ngOven temp.: 227°C to 305°C @ 8°C/min. (hold 10 min.)Inj. & det. temp.: 300°CCarrier gas: heliumLinear velocity: 35cm/sec. set @ 227°C (1.3mL/min.)FID sensitivity: 8 x 10-11 AFSSplitless hold time: 1.00 min.

1. cholesterol2. brassicasterol3. campesterol4. stigmasterol5. β-sitosterol6. avenasterol-d57. erythrodiol8. uvacol

GC_FF00131

12

3

min. 5 10 15 20

4

5

6

7

8

Phytosterols (Saw Palmetto)Rtx®-5

1. cholesterol2. brassicasterol3. campesterol4. stigmasterol5. β-sitosterol

60m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10226).1µL splitless injection*.Oven temp.: 200°C (hold 1 min.) to 340°C @ 15°C/min. (hold 10 min.)Inj./FID temp.: 345°C/355°C.

*Split injection may be used, but results can have greater variability. A split flow of112mL/min. is suggested.

1

2

3

4

5

min. 5 10GC_FF00368

Chromatogram provided by the Institute for Nutraceutical Advancement (INA)

min. 2 4 6 8 10

23

Sterols (Cholesterol)XTI®-5

1. 5-α-cholestane2. coprosterol3. cholesterol

1

30m, 0.25mm ID, 0.50µm XTI®-5 (cat.# 12238)1.0µL split injection, 250ng on-column

Oven temp.: 330°CInj. & det. temp.: 300°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 40°CFID sensitivity: 8 x 10-11 AFSSplit ratio: 100:1

GC_FF00132

min. 2 4 6 8 10

2

3

4

56

7

8

9

1

SterolsXTI®-5

30m, 0.25mm ID, 0.50µm XTI®-5 (cat.# 12238)1.0µL split injection.On-column conc.: 250ng

Oven temp.: 330°CInj. & det. temp.: 300°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 40°CFID sensitivity: 8 x 10-11 AFSSplit ratio: 100:1

GC_FF00133

1. 5-α-cholestane2. coprosterol3. cholesterol4. brassicasterol5. ergosterol6. campesterol7. stigmasterol8. β-sitosterol9. lanosterol

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652 Triglycerides; Sugars


Butter TriglyceridesRtx®-65TG

min. 0 10 20 30 40

T26 T28 T32 T34 T36 T38 T40 T42 T44 T46 T48 T50 T54T52

30m, 0.25mm ID, 0.10µm Rtx®-65TG (cat.# 17008)0.2µL cold on-column injection of 1% butterfat in isooctane.


Det. temp.: 380°CCarrier gas: hydrogenLinear velocity: 70cm/sec. FID sensitivity: 16 x 10-11 AFSSPI injector: high performance capillary insert 60°C,

300°C/min. to 400°C (hold 5 min.)

GC_FF00137

3 4 6 8

15m, 0.32mm ID, 0.20µm Rtx®-2330 (cat.# 10721)0.6µL split injection

Oven temp.: 175°C (hold 2 min.) to 240°C @ 8°C/min. (hold 1 min.) to 265°C @ 8°C/min. (hold 12 min.)

Inj. & det. temp.: 275°CCarrier gas: heliumLinear velocity: 80cm/sec. (flow rate: 10cc/min.)FID sensitivity: 2 x 10-11 AFSSplit ratio: 20:1

Sugars (as alditol acetates)Rtx®-2330

GC_FF00127

1 2 5 7 9 10

11

12 13

14

15

16

min. 2 4 6 8 10 12 14 16 18 20 22

1. glyceraldehyde2. deoxyribitol3. rhamnitol4. fucitol5. ribitol6. arabinitol7. xylitol8. deoxyglucitol9. mannitol

10. galactitol11. glucitol12. inositol13. glucoheptitol14. n-acetyl galactose amine15. n-acetyl glucose amine16. 2-keto-3-deoxyoctanate

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S653Sugars, Sugar Alcohols


5

Sugars (as alditol acetates)Rtx®-225

GC_FF00128

87

64

3

21

1. rhamnitol2. fucitol3. ribitol4. arabinitol5. mannitol6. galactitol7. glucitol8. inositol



Inj. & det. temp.: 260°CCarrier gas: hydrogenLinear velocity: 42cm/sec. set @ 40°CFID sensitivity: 16 x 10-11 AFSSplit ratio: 50:1

min. 2.5 5 7.5 10 12.5

Sugars (TMS Derivatives)3% Rt-101 on 100/120 Silcoport™

min. 5 10 15 20

46

5

3[2

1

3% Rt-101 on 100/120 Silcoport™ (cat.# 80400)2m, 1/8" OD x 2mm ID

Oven temp.: 140°C to 230°C @ 5°C/min. (hold 5 min.)Inj./det. temp.: 250°C / 300°CDet.: FIDCarrier gas: helium Column flow rate: 35mL/min. FID sensitivity: 1.28 x 10-10 AFS

1. l-arabinose2. ribose anomers3. d-xylose4. d-fructose5. d-galactose6. α-d-glucose

GC_FF00129

Sugar Alcohols (TMS derivatives)3% Rt-101 on 100/120 Silcoport™

1

2

3

4

5

6

7

8

3% Rt-101 on 100/120 Silcoport™ (cat.# 80400)2m, 1/8" OD x 2mm ID

Oven temp.: 140°C to 230°C @ 5°C/min. (hold 5 min.)Inj./det. temp.: 250°C / 300°CDet.: FIDCarrier gas: helium Column flow rate: 35mL/min. FID sensitivity: 1.28 x 10-10 AFS

1. l-arabinose2. xylitol3. d-arabinitol4. d-mannose5. l-sorbose6. d-glucose7. d-sorbitol8. inositol

GC_FF00130min. 5 10 15 20

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S654 Blood Alcohol


Blood AlcoholRtx®-BAC1 & Rtx®-BAC2 (0.32mm ID Columns)(headspace analysis)

Dual-column analysis using a two-hole ferrule. 1.0mL headspace sample of a blood alcohol mixOven temp.: 40°CInj. temp.: 200°CCarrier gas: heliumSample equilibration: 70°C, 15 min.Vial pressure: 30psiVial pressurization time: 0.15 min.Vial sampling time: 0.01 min.

Transfer line: 0.32mm ID Hydroguard fused silicatubingTransfer line temp.: 200°CInjection port sleeve: 2mm IDSplit flow: 20mL/min.

CCoonncc..ww//vv

1. methanol 0.1%2. acetaldehyde 0.2%3. ethanol 0.2%4. isopropanol 0.1%5. acetone 0.01%6. n-propanol 0.1%

30m, 0.32mm ID, 1.8µm Rtx®-BAC1 (cat.# 18003) 30m, 0.32mm ID, 1.2µm Rtx®-BAC2 (cat.# 18002)

min. 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0

6

5

4

3

2

1

6

5

4

3

2

1

GC_PH00241

min. 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0

Blood AlcoholRtx®-BAC1 & Rtx®-BAC2 (0.53mm ID columns)(headspace analysis)

65

1

2

3

4

1. methanol2. acetaldehyde3. ethanol4. isopropanol5. acetone6. n-propanol

Rtx®-BAC1 30m, 0.53mm ID, 3.0µm (cat.# 18001)

min. 1 2 3

1

2

3

4

5

6

Rtx®-BAC2 30m, 0.53mm ID, 2.0µm (cat.# 18000)

1.0mL headspace sample of a blood alcohol mixSample conc.: 0.1% per compound

Oven temp.: 40°CInj. & det. temp.: 200°CCarrier gas: heliumLinear velocity: 80cm/sec. set @ 40°CFID sensitivity: 1.28 x 10-10 AFS

GC_PH00239

min. 1 2 3

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655Blood Alcohol


Blood Alcohol5% Carbowax® 20M on 80/120 CarboBlack™ B(headspace analysis)

6 ft x 1/8", 2.0mm ID SilcoSmooth™ tubing5% Carbowax 20M on 80/120 CarboBlack™ B (cat.# 80105)Inj.: 500µL, headspaceConc.: 0.05gm/dL

Oven temp.: 90°CInj. & det. temp.: 200°CCarrier gas: heliumFlow rate: 18mL/min.

1. acetaldehyde2. methanol3. acetone4. ethanol5. isopropanol6. tert-butanol

GC_PH00478

a plus 1 storyRon developed a program that allows our AppliedScience Group to query by several parametersand retrieve information in a timely and usefulformat, enabling the group to make data-baseddecisions quickly.

RRoonn ZZhhaaoo,, Programmer/Analyst

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S656 Drugs of Abuse


PhencyclidineRtx®-5

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)1.0µL split injection of phencyclidineConc.: 1000ng/µLOven temp.: 50°C (hold 1 min.) to 250°C @ 25°C/min.,

then to 325°C @ 10°C/min. (hold 2 min.)Inj. / det. temp.: 250°C / 325°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 50°CFID sensitivity: 2.56 x 10-10 AFSSplit ratio: 30:1

min. 2 4 6 8 10 12 14 16 18

GC_PH00234

1. phencyclidine

1

1. cannabidiol2. ∆8-tetrahydrocannabinol3. ∆9-tetrahydrocannabinol4. cannibinol5. 11-hydroxy-∆9-tetrahydrocannabinol6. 11-nor-∆9-tetrahydrocannabinol carboxylic acid7. 11-nor-∆8-tetrahydrocannabinol carboxylic acid

Cannabinoids (TMS Derivatives)Rtx®-5

12

3

4

5

67

min. 5 6 7 8 9 10 11 12 13 14 15

GC_PH00253

15m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10220)1.0µL splitless injection of cannabinoidsConc.: 100µg/mL

Oven temp.: 50°C (hold 0.5 min.) to 225°C @ 30°C/min., to 325°C @ 10°C/min.

Inj. temp.: 225°CInterface temp.: 320°CDet.: MSDIonization: EI Carrier gas: heliumScan range: 40-500 AMULinear velocity: 40cm/sec. set @ 50°CSplitless hold time: 0.75 min.

Cocaine & Metabolites (TMS Derivatives)Rtx®-5

min. 2 4 6 8 10 12 14 16

GC_PH00254

2

1

5

34

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)1.0µL split injection of cocaine and cocaine metabolites

Oven temp.: 150°C to 320°C @ 10°C/min.Inj. / det. temp.: 250°C / 300°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 50°CFID sensitivity: 2.56 x 10-10 AFSSplit ratio: 30:1

1. ecgonine methyl ester (TMS)2. ecgonine (TMS)3. cocaine4. coca ethylene5. benzoylecgonine (TMS)

Opiates (TMS Derivatives)Rtx®-5

1 2 3

4

5

6

7,8

9 10

11

min. 4 5 6 7 8 9 10 11 12

GC_PH00233

1. meperidine2. alphaprodine3. methadone4. levorphanol (TMS)5. codeine (TMS)6. hydrocodone7. morphine (TMS)8. hydromorphone (TMS)9. oxycodone (TMS)

10. oxymorphone (TMS)11. nalorphine (TMS)

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)2.0µL split injection of opiatesConc: 2000ng/µLOven temp.: 200°C to 325°C @ 7°C/min.Inj. / det. temp.: 250°C / 300°CDet. type: MSIonization: EICarrier gas: heliumMode: full scanLinear velocity: 30cm/sec. set @ 200°CSplit ratio: 50:1

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657Anesthethetics; Solvents


1. isoflurane2. enflurane3. halothane4. methoxyflurane

1.0mL headspace sample Oven temp.: 40°C (hold 5 min.) to 240°C @ 5°C/min.Inj. & det. temp.: 240°CCarrier gas: heliumLinear velocity: 65cm/sec.

AnestheticsRtx®-BAC1 & Rtx®-BAC2(headspace analysis)

GC_PH00501 GC_PH00502


1.0mL headspace sample Oven temp.: 40°C (hold 5 min.) to 240°C @ 5°C/min.Inj. & det. temp.: 240°CCarrier gas: heliumLinear velocity: 65cm/sec.

1. ethyl chloride2. diethyl ether3. methylene chloride4. acetonitrile5. methyl tert-butyl ether6. chloroform7. methyl ethyl ketone8. 1,1,1-trichloroethane9. benzene

10. trichloroethylene11. toluene12. 1,1,2-trichloroethane13. tetrachloroethene14. p-xylene15. m-xylene16. o-xylene17. tetrachloroethane

SolventsRtx®-BAC1 & Rtx®-BAC2(headspace analysis)


GC_PH00509 GC_PH00510

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S658 Abused Inhalants


Abused InhalantsRtx®-BAC1 & Rtx®-BAC2

30m, 0.32mm ID, 1.80µm Rtx®-BAC1 (cat.# 18003)30m, 0.32mm ID, 1.20µm Rtx®-BAC2 (cat.# 18002)Oven temp.: 40°C (hold 4 min.) to 120°C @ 10°C/min.Carrier gas: heliumLinear velocity: 50cm/sec.Detector: FIDSample: 250µL headspaceTransfer line temp.: 125°CSample loop temp.: 125°CVial temp.: 70°C

min. 1 2 3 4 5 6 7 8 9 10 11

1

Rtx®-BAC1

2

3

4

5

6

7

8

9

10/11

12

13

14

15

1. methanol2. ethanol3. diethyl ether4. isopropanol5. methylene chloride6. acetone7. methyl tert-butyl ether8. hexane9. chloroform

10. ethyl acetate11. methyl ethyl ketone12. benzene13. trichloroethylene14. toluene15. methyl isobutyl ketone

Acknowledgement: Analyses performed using a Tekmar 7000 Headspace Autosampler on loan courtesy of Tekmar-Dohrmann.

GC_PH00446

1

2

3

4

5

6

7

8

9,11

10

12

13

14

15

Rtx®-BAC2

min. 1 2 3 4 5 6 7 8 9 10 11

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659Arson Accelerants


Unleaded GasolineRtx®-1

30m, 0.53mm ID, 1.50µm Rtx®-1 (cat.# 10170) Oven temp.: 40°C (hold 3 min.) to 75°C @

15°C/min. to 275°C @ 20°C/min. (hold 5 min.) Inj./det. temp.: 250°C/285°CCarrier gas: hydrogen Linear velocity: 50cm/sec. set @ 40°C FID sensitivity: 4.10 x 10-9 AFSSplit ratio: 30:1

min. 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 min. 4 8 12 16 20 24 28 32 36 40 44

Unweathered 99% Weathered

GC_MS00270

30m, 0.53mm ID, 1.0µm Rtx®-5 (cat.# 10255)1.0µL split injection of Motor Oil Composite Standard (cat.# 31464)*Conc.: 50,000ppmOven temp.: 40°C (hold 2 min.) to 330°C @ 10°C/min. (hold 60 min.)Inj./det. temp.: 300/330°CCarrier gas: hydrogen Linear velocity: 41cm/sec.Split ratio: 3:1

min. 10 20 30 40 50 60

GC_MS00272

Motor OilRtx®-5

*Prepared from an equal volume blend of 5w30, 10w30, 10w40, and 20w50 motor oil.A precisely weighed amount of the composite is added to a volumetric flask to pro-duce a mixture at 50,000µg/mL in methylene chloride, 1mL/ampul.

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)1.0µL split injection of Used Motor Oil Composite Standard (cat.# 31465)*Conc.: 50,000ppmOven temp.: 40°C to 340°C @ 10°C/min. (hold 15 min.)Inj./det. temp.: 250/340°CCarrier gas: hydrogen Linear velocity: 40cm/sec.Split ratio: 15:1

min. 10 20 30 40 50 60

GC_MS00273

Motor Oil (Used)Rtx®-5

*Prepared from an equal volume blend from five gasoline powered vehicles. A preciselyweighed amount of the composite is added to a volumetric flask to produce a mixture at50,000µg/mL in methylene chloride, 1mL/ampul.

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S660 Arson Accelerants


min. 10 20 30 40 50 60 70

KeroseneRtx®-1

GC_MS00274

30m, 0.32mm ID, 1.5µm Rtx®-1 (cat.# 10169)1.0µL split injection of a kerosene standard (cat.# 31094)Conc.: 5000µg/mL Oven temp.: 40°C (hold 5 min.) to 300°C @

4°C/min. (hold 5 min.)Inj. / det. temp.: 275°C / 300°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 40°CSplit ratio: 30:1

min. 10 20 30 40 50 60 70

Diesel FuelRtx®-1

30m, 0.32mm ID, 1.5µm Rtx®-1 (cat.# 10169)1.0µL split injection of a diesel fuel #2 standard (cat.# 31093)Conc.: 5000µg/mL


Inj. / det. temp.: 275°C / 300°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 40°CSplit ratio: 30:1

GC_MS00275

min. 10 20 30 40 50 60 70GC_MS00276

Mineral Spirits Rtx®-1

30m, 0.32mm ID, 1.5µm Rtx®-1 (cat.# 10169)1.0µL split injection of a mineral spirits standard (cat.# 31225)Conc.: 5000µg/mL Oven temp.: 40°C (hold 5 min.) to 300°C @

4°C/min. (hold 5 min.)Inj. / det. temp.: 275°C / 300°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 40°CSplit ratio: 30:1

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661Cleaning Solvents


Cleaning SolventsRtx®-Wax

Rtx®-Wax, 30m, 0.32mm ID, 0.25µm (cat.# 12424)Sample: 1% each solvent in methanolInj.: 1.0µL split (split ratio 100:1), 4mm inlet liner (cat.# 20814)Inj. temp.: 250°CCarrier gas: hydrogen, constant flow Flow rate: 3.0 mL/min.Oven temp.: 35°C (4 min. hold) to 85°C @ 3°C/min., to 200°C @ 15°C/min. (2 min. hold)Det.: FID @ 250°C

1. methanol2. iso-propanol3. ethanol4. n-propanol5. iso-butanol6. n-butanol7. methyl Cellosolve®

8. limonene9. Cellosolve®

10. butyl Cellosolve®

11. dipropylene glycol monomethyl ether (DPGMME)12. diethylene glycol ethyl ether (DEGEE)13. ethylene glycol14. tripropylene glycol monomethyl ether (TPGMME)15. phenyl Cellosolve®

GC_FF00644

Cleaning SolventsRtx®-VMS

Rtx®-VMS, 60m, 0.25mm ID, 1.4µm (cat.# 19916)Sample: 1% each solvent in methanolInj.: 1.0µL split (split ratio 100:1), 4mm inlet liner (cat.# 20814)Inj. temp.: 250°CCarrier gas: hydrogen, constant flow Flow rate: 1.5 mL/min.Oven temp.: 60°C (1 min. hold) to 138°C @ 4°C/min.,

to 145°C @ 2°C/min., to 210°C @ 15°C/min., to 230°C @ 5°C/min.

Det.: FID @ 250°C

1. methanol2. ethanol3. iso-propanol4. tert-butanol5. n-propanol6. iso-butanol7. methyl Cellosolve®

8. n-butanol9. Cellosolve®

10. ethylene glycol11. butyl Cellosolve®

12. limonene13. dipropylene glycol monomethyl ether (DPGMME)14. diethylene glycol ethyl ether (DEGEE)15. phenyl Cellosolve®

16. tripropylene glycol monomethyl ether (TPGMME)

GC_FF00645

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S662 Cleaning Solvents; Fragrances


Cleaning Solvents (All-Purpose Cleaner)Rtx®-VMS

Rtx®-VMS, 60m, 0.25mm ID, 1.4µm (cat.# 19916)Sample: approx. 5% cleaner in methanolInj.: 1.0µL split (split ratio 100:1), 4mm inlet liner (cat.# 20814)Inj. temp.: 250°CCarrier gas: hydrogen, constant flow Flow rate: 1.5 mL/min.Oven temp.: 60°C (1 min. hold) to 138°C @ 4°C/min. to 145°C @ 2°C/min. to 210°C

@ 15°C/min. to 230°C @ 5°C/min.Det.: FID @ 250°C

1. butyl Cellosolve®

2. limonene

GC_FF00646

Cleaning Solvents (Glass Cleaner)Rtx®-VMS

Rtx®-VMS, 60m, 0.25mm ID, 1.4µm (cat.# 19916)Sample: approx. 5% cleaner in methanolInj.: 1.0µL split (split ratio 100:1), 4mm inlet liner (cat.# 20814)Inj. temp.: 250°CCarrier gas: hydrogen, constant flow Flow rate: 1.5 mL/min.Oven temp.: 60°C (1 min. hold) to 138°C @ 4°C/min. to 145°C @ 2°C/min. to 210°C

@ 15°C/min. to 230°C @ 5°C/min.Det.: FID @ 250°C

1. iso-propanol2. butyl Cellosolve®

GC_FF00647

Personal Care Product FragrancesRtx®-1

Rtx®-1, 60m, 0.25mm ID, 0.25µm (cat.# 10126)Sample: 130ppm eucalyptus oil, 250ppm camphor,

260ppm menthol in methanolInj.: 1.0µL split (split ratio 20:1), 4mm inlet liner (cat.# 20814)Inj. temp.: 275°CCarrier gas: helium, constant flow Flow rate: 0.6 mL/min.Oven temp.: 80°C to 180°C @ 5°C/min.Det.: FID @ 300°C

1. eucalyptol2. camphor3. menthol

GC_FF00633

free literatureAAnnaallyyzziinngg CClleeaanniinngg aanndd PPeerrssoonnaall CCaarree PPrroodduuccttss bbyy GGaass aanndd LLiiqquuiiddCChhrroommaattooggrraapphhyy((lliitt.. ccaatt..## 5599773388))

Soaps, detergents, solvents, and many other components of house-hold cleaners and personal care products are effectively monitoredby GC and/or HPLC. Sometimes either approach can be used for thesame purpose, but one will offer time, accuracy, or economic advan-tages over the other. Sometimes the approach is clear, but the choiceinvolves alternative stationary phases. This 16-page guide presentsnumerous examples of GC and HPLC analyses of these diverse products.

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S663Permanent Gases


ShinCarbon ST 100/120 mesh2 meter x 1mm ID micropacked (cat.# 19808)Sample: 5µL permanent gases mix, approx. 5 mol. percent each

Inj. temp.: 100°CCarrier gas: heliumFlow rate: 10mL/min.Oven temp.: 40°C (hold 3 min.) to 250°

@ 8°/min. (hold 10 min.)Det. HID @ 200°C

Permanent GasesShinCarbon ST(micropacked)

1. hydrogen2. oxygen3. nitrogen4. carbon monoxide5. methane6. carbon dioxide

GC_PC00666

ShinCarbon ST 100/120 mesh 2 meter x 1mm ID micropacked (cat.# 19808)Sample: Permanent gases-C1+C2 hydrocarbons Sample size: 5µLInj. temp.: 100°CCarrier gas: heliumFlow rate: 10mL/min.Oven temp.: 40°C (hold 3 min.) to 250°

@ 8°/min. (hold 10 min.)Det. HID @ 200°C

Permanent Gases, C1+C2 hydrocarbonsShinCarbon ST(micropacked)

1. oxygen2. nitrogen3. carbon monoxide4. methane5. carbon dioxide6. acetylene7. ethylene8. ethane

GC_PC00665

Permanent GasesRt-Msieve™ 5A PLOT

1

6

5

3

4

min. 1 2 3 4

15m, 0.32mm ID Rt-Msieve™ 5A PLOT (cat. # 19720)Sample conc.: 2-5 mol%

Column temp.: 70°CCarrier gas: hydrogenLinear velocity: 64cm/sec.Inj. temp.: 70°C Det.: HP µTCDDet. temp.: 200°C Sensitivity: high

1. helium2. argon3. oxygen4. nitrogen5. methane6. carbon monoxide

2

GC_PC00165


www.restek.com

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664 Permanent Gases


Permanent GasesMolecular Sieve 5A and Molecular Sieve 13X

Molecular Sieve 5A: excellent resolution of oxygen, nitrogen,

methane, and carbon monoxide.

1. oxygen2. nitrogen3. methane4. carbon monoxide

10µL helium, 5–10% each componentOven temp.: 50 °CInj./det. temp.: 150 °C/200°CCarrier gas: hydrogenFlow: 30mL/min., heliumDetector: TCD

1 2

3 4

min.0 2 4

Molecular Sieve 13X: rapid analysisand excellent peak symmetry for

carbon monoxide.

GC_PC00166

1

2

3

4

min.0 2 4

Molecular Sieve 5A 80/100 mesh1m x 1/8" x 2mm ID SilcoSmooth™ tubing (cat.# 80440-800)

Molecular Sieve 13X 80/100 mesh2m x 1/8" x 2mm (ID) SilcoSmooth™ tubing (cat.# 80439-800)

Permanent GasesRt-QPLOT™

30m, 0.32mm ID Rt-QPLOT™ PLOT (cat.# 19718)Sample: 30µL split injection (40:1), 2-5 mol%

Column temp.: 30°CInj. temp.: 30°CDet.: HP µTCDDet. temp.: 200°C Carrier gas: hydrogen Linear velocity: 38cm/sec. Split flow: 40:1

1. helium2. air3. carbon monoxide4. methane5. carbon dioxide

min. 1 2

2

31

4

5

30°C

GC_PC00169

Permanent GasesRt-MSieve™ 5A PLOT

30m, 0.53mm ID, 50µm Rt-Msieve™ 5A PLOT (cat.# 19723) Sample: 0.5mL (sample loop injection)

Column temp.: 27°C Carrier gas: helium Linear velocity: 34cm/sec.Det.: Valco HID

1. hydrogen, 29.1 ppm2. argon, 53.4 ppm3. oxygen, 31.3 ppm

1

2

3

min. 1 2 3

Chromatogram courtesy of Larry McElmurry, Mobile Analytical Labs.

GC_PC00170

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S665Permanent Gases


Permanent Gases Rt-QPLOT™

1. nitrogen 2. oxygen3. argon

1. air 2. carbon monoxide3. methane4. carbon dioxide

Sample conc.: 2-5 mol%Inj./det. temp.: -40°CCarrier gas: hydrogenLinear velocity: 20cm/sec.Split flow: 40:1

min. 1 2 3

1 2

3

4

1

2

3Subambient Separation:

15°C

Subambient Separation:

-40°C

Sample conc.: 2-5 mol%Inj./det. temp.: 15°CCarrier gas: hydrogenLinear velocity: 34cm/sec.Split flow: 40:1

30m, 0.32mm ID Rt-QPLOT™ PLOT column (cat. # 19718)

min. 3 4GC_PC00174

[[

0.40 0.60 0.80

1

2

2, 3, 4, 7

56

3A—Rt-QPLOT™ B—Rt-Msieve™ 5A

[1

2

3

4

5

7

Oven temp.: 30°C Inj.: 20µL, split injection (10:1),

5% each compound Carrier gas: hydrogen, 8psi Det.: HP µTCDDet. temp.: 200°C

1. helium2. argon3. oxygen4. nitrogen5. methane6. carbon dioxide7. carbon monoxide

B

Terrific separation!

Rt-QPLOT™ Rt-Msieve™ 5A

Permanent GasesRt-QPLOT™ and Rt-Msieve™ 5A (Dual Column Configuration)

A

min. 2 4 6 8 10 12 14 16

Injector

Universal ‘Y’ Press-Tight®

Connector (cat.# 20406)

Rt-Msieve™ 5A Column(30m, 0.32mm ID, cat.# 19722)

Rt-QPLOT™ Column(30m, 0.53mm ID, cat.# 19716)

50cm from Rt-QPLOT™ Column

Detector

Universal ‘Y’ Press-Tight®

Connector (cat.# 20406)

50cm from Rt-QPLOT™ Column

Fast, complete separation ofgases and volatile compounds!

No complicated valve-switching.

2.20

GC_PC00171

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666 Hydrocarbon Gases


Hydrocarbon GasesRt-QPLOT™

1. methane2. ethylene3. acetylene4. ethane5. propylene6. propane7. cyclopropane8. propadiene9. propyne

10. isobutane11. 1,3-butadiene12. 1-butene13. n-butane14. cis-2-butene15. trans-2-butene16. isopentane17. n-pentane

30m, 0.32mm ID Rt-QPLOT™ PLOT (cat.# 19718)300µL split injection (40:1), 1000ppm

Oven temp.: 50°C (2 min.) to 220°C @ 15°C/min. Inj./det. temp.: 250°C Carrier gas: helium Linear velocity: 42cm/sec. set @ 80°C (5.6mL/min.) Split flow: 40mL/min. FID sensitivity: 1.28 x 10-10 AFS

4

2,3

5

6

7

8,9

10

11

12

13

14

15

16

17

1

min. 1 2 3 4 5 6 7 8 9 10 11 12 13 14

GC_PC00176

423

5,6

7

8

9

1

min. 1 2 3 4 5 6 7 8 9 10 11

Hydrocarbon GasesRt-UPLOT™ 1. methane

2. ethylene3. ethane4. acetylene5. propane6. propylene7. cyclopropane8. propadiene9. propyne

10. isobutane11. n-butane12. 1-butene13. cis-2-butene14. trans-2-butene15. 1,3-butadiene16. isopentane17. n-pentane

30m, 0.32mm ID Rt-UPLOT™ PLOT (cat.# 19724)300µL split injection (40:1), 1000ppm

Oven temp.: 50°C to 190°C @ 10°C/min. Inj./det. temp.: 250°C Carrier gas: helium Linear velocity: 42cm/sec. set @ 80°C (5.6mL/min.) Split flow: 40mL/min.FID sensitivity: 1.28 x 10-10 AFS

GC_PC00177

10

11

12

13

14

15

16

17

Hydrocarbon GasesRt-Msieve™ 13X PLOT

30m, 0.53mm ID Rt-Msieve™ 13X PLOT (cat.# 19706) Sample conc.: 2-5 mol%

Oven temp.: 30°C (hold 2 min.) to 300°C @ 15°C/min. (hold 10 min.) Carrier gas: hydrogen Linear velocity: 30cm/sec.

1. argon/oxygen2. nitrogen3. C14. carbon monoxide5. C26. C37. C4

min. 2 4 6 8 10 12 14 16 18 20 22 24 26

1

2

4

5

3

6

7

GC_PC00168

ShinCarbon ST 100/120 mesh (cat.# 19809)1 meter x 1mm ID Silcosteel® micropacked column

Sample: fluorocarbon blend, 5µL ~1-3% eachInj. temp.: 200°CDet.: FID/250°CCarrier gas: helium Linear velocity: 10mL/min. Oven temp.: 125°C to 320°C @ 16°C/min.

Peak Ret. Time (min.)1. Freon® 134a/22 4.952. Freon® 12 7.113. Freon® 133a 9.194. Freon® 114 10.85

FluorocarbonsShinCarbon ST(micropacked)

GC_PC00673

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S667


Hydrocarbon Gases


30m, 0.32mm ID Rt-QPLOT™ (cat.# 19718)30.0µL injection of Hydrocarbon Gas Mix

Oven temp.: 40°C to 240°C @ 8°C/min. (hold 10 min.)Inj.: split/250°CCarrier gas: helium (constant pressure mode)Head pressure: 18.0psi Column flow rate: 1.5cc/min. @ 40°CLinear velocity: 35cm/sec. @ 40°CSplit ratio: 20:1Det.: FID/240°CMake-up gas flow: 45cc/min.Inlet liner: 4mm single gooseneck (cat.# 20798)

1. methane2. ethene3. ethane4. propene5. propane6. propadiene7. isobutane8. 1,3-butadiene9. 1-butene

10. isobutene11. n-butane12. cis-2-butene13. trans-2-butene14. isopentane15. n-pentane16. n-hexane

GC_PC00521



Oven temp.: 35°C to 240°C @ 10°C/min. (hold 10 min.)Injector: split/250°CCarrier gas: helium (constant pressure mode)Head pressure: 18.0psi Column flow rate: 1.5cc/min. @ 40°CLinear velocity: 35cm/sec. @ 40°CSplit ratio: 20:1Det.: FID/240°CMake-up gas flow: 45cc/min.Inlet liner: 4mm single gooseneck (cat.# 20798)

1. methane2. ethylene3. ethane4. propylene5. propane6. isobutane7. 1,3-butadiene8. 1-butene9. isobutene

10. n-butane11. cis-2-butene12. trans-2-butene

GC_PC00522

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1. methane2. ethane3. propane4. isobutane5. n-butane6. isopentane7. n-pentane8. hexanes


GC_PC00523


Oven temp.: 40°C to 240°C @ 10°C/min. (hold 10 min.)Injector: split/250°CCarrier gas: helium (constant pressure mode)Head pressure: 18.0psi Column flow rate: 1.5cc/min. @ 40°CLinear velocity: 35cm/sec. @ 40°CSplit ratio: 20:1Det.: FID/240°CMake-up gas flow: 45cc/min.Inlet liner: 4mm single gooseneck (cat.# 20798)

ShinCarbon ST 100/120 mesh (cat.# 19809)1 meter x 1mm ID Silcosteel® micropacked column

Sample: 0.5-8% varied, C1-C6 hydrocarbonsInj. temp.: 200°CDet.: FID/250°CCarrier gas: helium (constant flow)Linear velocity: 10mL/min. Oven temp.: 125°C to 250°C @ 16°C/min.

1. C12. C23. C34. iC4-isobutane5. C46. iC5-isopentane7. nC58. hexanes

Natural GasShinCarbon ST(micropacked)

GC_PC00672

free literatureSShhiinnCCaarrbboonn SSTT MMiiccrrooppaacckkeedd GGCC CCoolluummnnss::AAbboovvee--AAmmbbiieenntt AAnnaallyysseess ooff PPeerrmmaanneennttGGaasseess aanndd LLiigghhtt HHyyddrrooccaarrbboonnss

(lit. cat.# 59519A)

A ShinCarbon ST micropacked column willseparate hydrogen, oxygen, nitrogen, car-bon dioxide, carbon monoxide, and methanein 10 minutes, without cryogenic cooling - aseparation that has been impossible for asingle GC or GSC column without sub-ambi-ent temperature. The 2-page note alsoshows separations of light hydrocarbon /permanent gas mixtures, natural gas com-ponents, sulfur dioxide, and fluorocarbons -all obtained using a 1-meter or 2-metermicropacked column and above-ambient ini-tial temperatures.

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S669Hydrocarbon Gases


30m, 0.32mm ID (Rt-QPLOT™: cat.# 19718; Rt-SPLOT™: cat.# 19710; Rt-UPLOT™: cat.# 19724)100µL split injection. 1000ppm (v/v) each in helium, Cyclosplitter® liner (cat.# 20755).

Oven temp.: 50°CInj./det. temp.: 200°CCarrier gas: hydrogenDet.: FIDSplit ratio: 20:1

1. methane2. ethylene3. ethane4. acetylene5. propylene6. propane

Hydrocarbon GasesPorous Polymer PLOTs

Rt-QPLOT™ column - least selective for functional groups.

1 2

3 4

5,6

1

24,3

5

6

1

2,4

3

56

min. 1 2 3 4 5

min. 1 2 3 4

min. 1 2 3 4 5 6 7 8 9

Rt-SPLOT™ column - selective for polar, unsaturated compounds.

Rt-UPLOT™ column - most selective for unsaturated compounds.

GC_PC00180

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Refinery Gas Rt-Alumina™ PLOT

17

14

16

min. 2 4 6 8 10 12 14

1513

12

11

10

9

8

7

6

5

1

3

4

2

1. methane2. ethane3. ethylene4. propane5. cyclopropane6. propylene7. isobutane8. n-butane9. propadiene

10. acetylene11. trans-2-butene12. 1-butene13. cis-butene14. isopentane15. n-pentane16. 1,3-butadiene17. propyne

50m, 0.53mm ID Rt-Alumina™ PLOT (cat. # 19701)100µL hydrocarbon mix split injection , 1000ppm (gas-tight syringe)

Oven temp.: 40°C to 120°C @ 5°C/min. (hold 5 min.)Inj. & det. temp.: 200°CCarrier gas: heliumLinear velocity: 37.5cm/sec. set @ 80°C (5.0mL/min.)Split flow: 60mL/min.FID sensitivity: 1.28 x 10-10 AFS

GC_PC00183

Propylene PurityRt-Alumina™ PLOT

1

2

3

4

5

6

78

9

10

1112

1314

1516

17

min. 2 4 6 8 10 12 14

GC_PC00185

50m, 0.53mm ID Rt-Alumina™ (cat.# 19701)100µL hydrocarbon mix (gas-tight syringe)


Inj. & det. temp.: 200°CCarrier gas: heliumLinear velocity: 37.5cm/sec. set @ 80°CSplit flow: 60mL/min.FID sensitivity: 1.28 x 10-10 AFS


10. acetylene11. trans-2-butene12. 1-butene13. cis-2-butene14. isopentane15. n-pentane16. 1,3-butadiene17. propyne

Ethylene PurityRt-Alumina™ PLOT

3

1

2

50m, 0.53mm ID Rt-Alumina™ PLOT (cat. # 19701)200µL gas-tight syringe injection Sample conc.: 99+% ethylene

Oven temp.: 40°CInj. & det. temp.: 200°CCarrier gas: heliumLinear velocity: 42cm/sec. set @ 80°C (5.6mL/min.)Split flow: 40mL/min.FID sensitivity: 1.28 x 10-10 AFS

1. methane2. ethane3. ethylene

min. 2 4

GC_PC00184

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S671


Hydrocarbon Gases

1. methane2. ethane3. propane4. isobutane5. n-butane6. trans-2-butene7. 1-butene8. isobutylene9. cis-2-butene

10. isopentane11. 1,3-butadiene

1,3-Butadiene PurityRt-Alumina™ PLOT

3

4

2

50m, 0.53mm ID Rt-Alumina™ PLOT (cat. # 19701)500µL gas-tight syringe injectionSample conc.: 99+% 1,3 butadiene


min. 2 4 6 8 10 12

1

7 865

11

10

9

GC_PC00186

Butane FuelRtx®-1

1

2 3 4

1. methane2. propane3. isobutane4. butane

min. 2 4

GC_PC00204

60m, 0.53mm ID, 5.0µm Rtx®-1 (cat.# 10183)1.0µL butane fuel from a Microflame™ torchDirect injection using a Uniliner® liner

Oven temp.: 40°CInj. & det. temp.: 200°CCarrier gas: hydrogenLinear velocity: 80cm/sec. (flow rate: 10cc/min.)FID sensitivity: 4 x 10-11 AFS

Butane FuelRtx®-1

4

53

min. 2 4

1. methane2. ethane3. propane4. isobutane5. butane

GC_PC00205

60m, 0.53mm ID, 5.0µm Rtx®-1 (cat.# 10183)1.0µL butane fuel from a disposable lighterDirect injection using a Uniliner® liner


2

1

Butane FuelMXT®-1

43

5

1 2

min. 2 4 6


60m, 0.53mm ID, 5.0µm MXT®-1 (cat.# 70183)1.0µL direct injection of butane fuel

Oven temp.: 40°CInj. & det. temp.: 200°CCarrier gas: heliumLinear velocity: 40cm/sec.FID sensitivity: 4 x 10-11 AFS

GC_PC00206

Hydrocarbon GasesHayeSep® S(micropacked)

2m, 0.75mm ID HayeSep S micropacked column (cat.# 19011)500µL split injection of a light hydrocarbon gas mixture


Inj. & det. temp.: 220°CCarrier gas: heliumFlow: 20mL/min. set @ 40°CFID sensitivity: 32 x 10-11 AFSSplit ratio: 10:1

min. 4 8 12

7

65

4

32

1

GC_PC00187

1. methane2. ethylene3. acetylene4. ethane5. propylene6. propane7. 1-butene

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Propane Fuel Rtx®-1

60m, 0.53mm ID, 5.0µm Rtx®-1 (cat.# 10183)1.0µL propane fuelDirect injection using a Uniliner® inlet liner


1

3

4

2

5

min. 2 4

GC_PC00207


Methane PurityRtx®-1

60m, 0.53mm ID, 5.0µm Rtx®-1 (cat.# 10183)1/2cc slow injection (10 sec. duration) of ultra high purity methane Direct injection using a Uniliner® inlet liner


min. 4 8

2

1

GC_PC00208

1. methane2. trace hydrocarbon contaminants

11

826

9

7

10

12 14

1513

16

1

3

4 5

17

min. 2 4 6 8 10 12 14 16


10. acetylene11. trans-2-butene12. 1-butene13. cis-2-butene14. isopentane15. n-pentane16. 1,3-butadiene17. propyne

50m, 0.53mm ID, 6µm Rt-Alumina™ PLOT (cat. # 19701)50µL gas-tight syringe injection, 1000ppm each analyte


Hydrocarbon Test MixRt-Alumina™ PLOT

GC_PC00551

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S673Hydrocarbons


Gasoline AromaticsRtx®-1

1

6

13

22

2

3

4,5

7

8

11

9

12

15

14

18

20

2117

16

23

24

min. 10 15 20 25 30 35 40 45 50 55 60

19

GC_PC00214

10

1

2 3 4,5 6

7

8

9

11

10

12

13

14

15

16

17

18

19

20

21

22

23

24

min. 10 15 20 25 30 35 40 45 50 55 60GC_PC00215

1. benzene2. toluene3. ethylbenzene4. m-xylene5. p-xylene6. o-xylene7. isopropylbenzene8. n-propylbenzene9. 1-methyl-3-ethylbenzene

10. 1-methyl-4-ethylbenzene11. 1,3,5-trimethylbenzene12. 1-methyl-2-ethylbenzene13. 1,2,4-trimethylbenzene14. 1,2,3-trimethylbenzene15. indane16. 1,4-diethylbenzene17. butylbenzene18. 1,2-diethylbenzene19. 1,2,4,5-tetramethylbenzene20. 1,2,3,5-tetramethylbenzene21. pentamethylbenzene22. naphthalene23. 2-methylnaphthalene24. 1-methylnaphthalene

TIC

RIC

60m, 0.25mm ID, 1.0µm Rtx®-1 (cat.# 10156)1.0µL split injection of neat gasoline.


Inj. / det. temp.: 250°C / 280°CLinear velocity: 35cm/sec. set @ 50°CSplit ratio: 200:1Det.: MS, 45-300m/e, 1 scan/sec.

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674 Hydrocarbons


Gasoline Aromatics (ASTM D-5580) 20% TCEP on 80/100 Chromosorb® PAW(micropacked)

min. 10 20 30

1

2

3

4

1. benzene2. toluene3. 2-hexanone (ISTD)4. heavy hydrocarbons

20% TCEP on 80/100 Chromosorb® PAW0.56m, 0.75mm ID x 0.81mm OD micropacked (cat.# 19040)Sample: 0.5µL gasoline

Oven temp.: 60°C (hold 6 min.) to 115°C @ 2°C/min.Inj. & det. temp.: 250°CFlow rate: 4.4mL/min.

GC_PC00212

Gasoline Aromatics (ASTM D-5580)20% TCEP on 80/100 Chromosorb® PAW(micropacked)

1

2

4

3

}5

1. 2-hexanone (ISTD)2. ethylbenzene3. p/m-xylene4. o-xylene5. C9 + aromatics

GC_PC00213

20% TCEP on 80/100 Chromosorb® PAW30m, 0.53mm ID, 3.0µm Rtx®-1 (cat.# 10185)Sample: 0.5µL gasoline

Oven temp.: 60°C (hold 6 min.) to 115°C @ 2°C/min.Inj. & det. temp.: 250°CFlow rate: 2.8mL/min.

min. 10 20 30 40

Petroleum Oxygenates (ASTM D4815)TCEP (Micropacked) & Rtx®-1

1. methanol2. ethanol3. isopropanol4. tert-butanol5. n-propanol6. methyl tert-butyl ether7. sec-butanol8. diisopropyl ether (DIPE)9. isbutanol

10. ethyl-tert-butyl ether (ETBE)11. tert-amyl ether12. dimethoxyethane (DME)13. n-butanol14. benzene15. tert-amyl methyl ether

(TAME)16. total heavy hydrocarbons

min. 10 20

1

23

4 5

6

7

8

9

10

11

12

13

15

14

16

GC_PC00194

0.56m, 0.75mm ID 20% TCEP on Chromosorb® P A/W (cat.# 19040) and 30m, 0.53mmID, 3.0µm Rtx®-1 (cat.# 10185 connected in series)0.5µL split injection of oxygenates blend 1–10% wt in surrogate gasoline

Oven temp.: 60°CInj. / det. temp.: 200°C / 250°C (FID)Carrier gas: helium, 5mL/min. set @ 60°CSplit ratio: 15:1

Petroleum OxygenatesRt-TCEP™

1

2

3

4

56

78

9

60m, 0.25mm ID, 0.4µm Rt-TCEP™ (cat.# 10999)1.0µL split injection, components @ 500ppm.

Oven temp.: 60°C (hold 5 min.) to 100°C @ 5°C/min. (hold 10 min.)Inj. & det. temp.: 200°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 80°CFID sensitivity: 6.4 x 10-11 AFSSplit flow: 46mL/min.

1. methyl tert-butyl ether2. n-undecane3. tert-butanol4. methanol5. isopropyl alcohol6. ethanol7. n-dodecane8. n-tridecane9. n-butanol

GC_PC00195

min. 2 4 6 8 10 12 14 16

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Petroleum Oxygenates Stabilwax®

1

2

3

4

5

6 7

8

9 10

11

1312

14,15

16

17

18

1920 21

22

23

24

min. 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32GC_PC00196

30m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10655)0.2µL injection of a 15 to 30ng/µL synthetic blendDirect injection using a Uniliner® liner

Oven temp.: 45°C (hold 4 min.) to 220°C @ 6°C/min.Inj. & det. temp.: 220°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.1cc/min.)FID sensitivity: 16 x 10-11 AFS

1. heptane2. C3 oxide3. benzene4. toluene5. ethylbenzene6. p-xylene7. m-xylene8. cumene9. o-xylene

10. styrene11. 2-methylpentanol12. p-diethylbenzene13. m-diethylbenzene14. α-methylstyrene15. o-diethylbenzene16. phenylacetylene (trace)17. benzaldehyde18. monopropylene glycol19. acetophenone20. 2-phenyl-2-propanol21. α-methylbenzyl alcohol22. benzyl alcohol23. phenylethyl alcohol24. phenol

Petroleum Oxygenates (MTBE) Rtx®-1

1

2 3 4 5 6 7

8

9

min. 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34GC_PC00197

1. dimethylether2. methanol3. C4s4. tert-butyl alcohol5. methyl tert-butyl ether6. sec-butylmethylether7. diisobutylene8. 4-vinylcyclohexene-1 (BD1B dimer)9. triisobutylene

60m, 0.53mm ID, 5.0µm Rtx®-1 (cat.# 10183)0.1µL injection of neat methyl tert-butyl ether.Direct injection using a Uniliner® liner.

Oven temp.: 40°C (hold 8 min.) to 200°C @ 5°C/min.Inj. & det. temp.: 200°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5cc/min.)FID sensitivity: 8 x 10-11 AFS

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676 Hydrocarbons


1. benzene2. toluene3. ethylbenzene4. p-xylene5. m-xylene6. o-xylene

Aromatics (Benzene/Toluene/Xylene)MXT®-WAX

1

2

3 5

6

4

min. 4 8 12 16

GC_PC00188

30m, 0.28mm ID, 1.0µm MXT®-WAX (cat.# 70654)1.0µL split injection of BTEX Standard (cat.# 30051)

Oven temp.: 60°CInj. & det. temp.: 260°CCarrier gas: hydrogenLinear velocity: 38cm/sec.FID sensitivity: 3.2 x 10-11 AFSSplit ratio: 100:1

GC_PC00189

Aromatics (Benzene/Toluene/Xylene)Rtx®-200

min. 4 8

4,5

1

2

36

30m, 0.53mm ID, 0.50µm Rtx®-200 (cat.# 15040)0.1µL direct injection of BTEX Standard (cat.# 30051)

Oven temp.: 60°CInj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.2cc/min.)FID sensitivity: 4 x 10-11 AFS

1. benzene2. toluene3. ethylbenzene4. m-xylene5. p-xylene6. o-xylene

1. methyl tert-butyl ether2. benzene3. toluene4. ethylbenzene5. m/p-xylene6. o-xylene

3

5

64

min. 4 8 12 16 20

2

1

Aromatics (Benzene/Toluene/Xylene)Rtx®-502.2

GC_PC00190

105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910)0.25µL split injection of CA PVOC Mix (cat.# 30071)Conc.: 1000–5000µg/mL

Oven temp.: 40°C (hold 1 min. ) to 100°C @ 5°C/min., to 240°C @ 8°C/min. (hold 8 min.)

Inj. / det. temp.: 200°C / 280°CCarrier gas: heliumLinear velocity: 67cm/sec. set @ 40°CFID sensitivity: 2.05 x 10-9 AFSSplit flow: 70mL/min.

Aromatics (Benzene/Toluene/Xylene)Rtx®-Wax


min. 5 10 15 20

2

1

3 4 5

6

GC_PC00191

30m, 0.53mm ID, 1.0µm Rtx®-Wax (cat.# 12455)0.1µL direct injection of BTEX Standard (cat.# 30051)Oven temp.: 50°CInj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.2cc/min.)FID sensitivity: 16 x 10-11 AFS

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Aromatics (Xylene Isomers plus Cumene) 5% RT-1200/1.75% Bentone® 34 on 100/120 Silcoport™

1 2

3

4

56

7 8

9 10

11

min. 2 4 6 8 10 12 14

1. hexane2. benzene3. n-octane4. toluene5. n-nonane6. ethylbenzene7. p-xylene8. m-xylene9. o-xylene

10. cumene11. n-decane

5% RT-1200/1.75% Bentone® 34 on 100/120 Silcoport™ (cat.# 80125)1.8m, 1/8" OD x 2mm ID SilcoSmooth™ tubingSample size: 0.1µLConc.: 0.5µg/µL in hexaneOven temp.: 75°CInj. & det. temp.: 200°CCarrier gas: nitrogenFlow rate: 20mL/min.FID sensitivity: 32 x 10-11 AFS

GC_PC00192

Aromatics (Xylene Isomers /BTEX) 5% RT-1200/5% Bentone® 34 on 100/120 Silcoport™

1

2

34 5

6


5% RT-1200/5% Bentone® 34 100/120 Silcoport™ (cat.# 80129)2m, 1/8" OD x 2mm ID SilcoSmooth™ tubingSample size: 0.1µLConc.: 0.5µg/µL in hexaneOven temp.: 100°CInj. & det. temp.: 200°CCarrier gas: nitrogenFlow rate: 20mL/min.FID sensitivity: 32 x 10-11 AFS

GC_PC00193

min. 1 2 3 4 5 6 7

AromaticsRt-TCEP™

min. 0 2 4 6 8 10 12 14 16 18GC_PC00211

15

14

13

12

119

1087

6

5

421

3

1. n-undecane2. benzene3. n-dodecane4. toluene5. ethylbenzene6. p-xylene7. m-xylene8. cumene9. n-propylbenzene

10. o-xylene11. mesitylene12. 1-ethyl-2-methylbenzene13. m-diethylbenzene14. p-diethylbenzene15. o-diethylbenzene

60m, 0.25mm ID, 0.40µm Rt-TCEP™ (cat.# 10999)1.0µL split injection, components @ 500ppm (ethylbenzene @ 1000ppm).


Inj. & det. temp.: 200°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 80°CFID sensitivity: 6.4 x 10-11 AFSSplit flow: 46mL/min.

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678 Hydrocarbons


Aromatics, Aliphatics10% TCEP 100/120 Chromosorb® PAW

10% TCEP 100/120 Chromosorb® PAW (cat.# 80126)2.5m, 1/8" OD x 2mm ID SilcoSmooth™ tubingSample size: 0.1mL neatOven temp.: 80°CInj./det. temp.: 200°C/250°CCarrier gas: nitrogenFlow rate: 20mL/min.FID sensitivity: 128 x 10-10 AFS

1. n-hexane2. n-heptane3. n-octane4. n-nonane5. n-decane6. benzene7. toluene8. ethylbenzene

9a. m-xylene9b. p-xylene10. isopropylbenzene11. o-xylene

1 2 3 4

5

6

7

8

9

1011

min. 2 4 6 8 10 12 14 16GC_PC00217

Hydrocarbons, C7-C42Rtx®-1

min. 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60

12

3

4

5

79 11

12

13

14

1516

6 8 10 17 20

19

21

22

23

24

2526

27

30m, 0.53mm ID, 0.25µm Rtx®-1 (cat.# 10125)0.2µL injection of a synthetic hydrocarbon mix, ~ 0.1mg/mLper component Direct injection using a Uniliner® linerOven temp.: 40°C to 340°C @ 5°C/min.Inj. & det. temp.: 340°CCarrier gas: hydrogenLinear velocity: 40cm/sec. set @ 40°CFID sensitivity: 64 x 10-11 AFS

18

GC_PC00216

1. C72. C83. C94. C105. C116. C127. C138. C149. C15

10. C1611. C1712. C1813. C1914. C2015. C22

16. C2317. C2418. C2519. C2620. C2821. C3022. C3223. C3424. C3625. C3826. C4027. C42

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Hydrocarbons

1

2

3

4

9 10 11 12 min.

Rtx®-1PONA, 100m, 0.25mm ID, 0.5µm (cat.# 10195) w/ Rtx®-5 tuning column, 2.62m, 0.25mm ID, 1.0µm, connected via Press-Tight® connector (cat.# 20446)

Sample: custom detailed hydrocarbons analysis (DHA) mix, neat

Inj.: 0.01µL, split (split ratio 150:1),4mm cup inlet liner (cat.# 20709)

Inj. temp.: 200°CCarrier gas: helium, constant flowLinear velocity: 28cm/sec. (2.3mL/min.)Oven temp.: 35°CDet.: FID @ 250°C

1. ethanol2. C53. tert-butanol4. 2-methylbutene-2

C5 efficiency: 613,596 total theoretical platesk' (C5): 0.489tert-butanol skewness: 1.25Resolution (tert-butanol/2-methylbutene-2): 5.60

GC_PC00743A

Detailed Hydrocarbons AnalysisRtx®-1PONA

10 20 30 40 50 60 70 80 90 min.

1

23

4

5

6

7

8

911

10

12

13

14 15

16

17

18

1920

21

2223

24

2526

27

28

29 30

Rtx®-1PONA, 100m, 0.25mm ID, 0.5µm (cat.# 10195) w/ Rtx®-5 tuning column, 2.62m, 0.25mm ID, 1.0µm, connected via Press-Tight® connector (cat.# 20446)Sample: custom detailed hydrocarbons analysis

(DHA) mix, neatInj.: 0.01µL, split (split ratio 150:1), 4mm cup

inlet liner (cat.# 20709)Inj. temp.: 200°CCarrier gas: helium, constant flowLinear velocity: 28cm/sec. (2.3mL/min.)Oven temp.: 5°C (hold 15 min.) to 50°C @ 5°C/min.

(hold 50 min.) to 200°C @ 8°C/min. (hold10 min.)

Det.: FID @ 250°C

1. ethanol2. C53. tert-butanol4. 2-methylbutene-25. 2,3-dimethylbutane6. methyl tert-butyl ether (MTBE)7. C68. 1-methylcyclopentene9. benzene

10. cyclohexane11. 3-ethylpentane12. 1-tert-2-dimethylcyclopentane13. C714. 2,2,3-trimethylpentane15. 2,3,3-trimethylpentane

16. toluene17. C818. ethylbenzene19. p-xylene20. 2,3-dimethylheptane21. C922. 5-methylnonane23. 1,2-methylethylbenzene24. C1025. C11 (undecane)26. 1,2,3,5-tetramethylbenzene27. naphthalene28. C12 (dodecane)29. 1-methylnaphthalene30. C13 (tridecane)

Resolution (1-methylcyclopentane/benzene): 1.28

Circles indicatecritical pairs that must beresolved.

GC_PC00743

Detailed Hydrocarbons AnalysisRtx®-1PONA

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ReformateRtx®-1

GC_PC00210

min. 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42

1

2

3

4

5

6 7,8

9

10

11

1213

14

15

16

17

18

19

2021

22

23

24 25 26

27

28

29

30 31 32

33

34,35

36

37

38 39

40

4142

43

44

45

46

55

54

53525150

49

4847

1. propane2. isobutane3. n-butane4. isopentane5. n-pentane6. 2,2-dimethylbutane7. cyclopentane8. 2,3-dimethylbutane9. 2-methylpentane

10. 3-methylpentane11. n-hexane12. 2,2-dimethylpentane13. methylcyclopentane14. 2,4-dimethylpentane15. benzene16. C7 paraffin17. C7 paraffin18. C7 paraffin19. toluene20. C8 paraffin21. C8 paraffin22. C8 paraffin23. ethylbenzene

24. m-xylene25. p-xylene26. o-xylene27. isopropylbenzene28. n-propylbenzene29. 1-methyl-3-ethylbenzene30. 1-methyl-5-ethylbenzene31. 1,3,5-trimethylbenzene32. 1-methyl-2-ethylbenzene33. 1,2,4-trimethylbenzene34. 1,2,3-trimethylbenzene35. 1-methyl-3-isopropylbenzene36. 1,3-diethylbenzene37. 1-methyl-3-n-propylbenzene38. 1-methyl-4-n-propylbenzene39. n-butylbenzene40. 1,2-diethylbenzene41. 1-methyl-2-n-propylbenzene42. 1,4-dimethyl-2-ethylbenzene43. 1,3-dimethyl-4-ethylbenzene44. 1,2-dimethyl-4-ethylbenzene45. 1,2,4,5-tetramethylbenzene46. 1,2,3,5-tetramethylbenzene

47. C10 indane48. C12 aromatic49. naphthalene50. C12 aromatic51. C12 aromatic52. C12 aromatic53. C12 aromatic54. 2-methylnaphthalene55. 1-methylnaphthalene

105m, 0.25mm ID, 0.50µm Rtx®-1 (cat.# 10144)1.0µL split injection of petroleum standards

Oven temp.: 30°C (hold 10 min.) to 200°C @1°C/min.

Inj. & det. temp.: 250°CCarrier gas: heliumLinear velocity: 20cm/sec. (flow rate: 0.7cc/min.)FID sensitivity: 8 x 10-11 AFSSplit Ratio: 100:1

min. 48 50 52 54 56 58 60 62 64 66 68 70 72 74 76 78 80 82 84 86 88

min. 90 92 94 96 98 100 102 104 106 108 110 112 114 116 118 120 126 128 130 132 134

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S681Hydrocarbons


Crude Oil, Prudhoe BayRtx®-1

2827

2625

23222120

19

18

17

1413

1211

10987

6

5

3

2

1

min. 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80

1. benzene2. heptane3. toluene4. octane

5a. m-xylene5b. p-xylene

6. o-xylene7. nonane8. decane9. undecane

10. dodecane11. tridecane12. tetradecane13. pentadecane14. hexadecane15. heptadecane16. pristane

17. octadecane18. phytane19. nonadecane20. eicosane21. heneicosane22. docosane23. tricosane24. tetracosane25. pentacosane26. hexacosane27. heptacosane28. octacosane

GC_PC00231

30m, 0.53mm ID, 3.0µm Rtx®-1 (cat.# 10185)0.4µL split injection of Prudhoe Bay crude oilOven temp.: 40°C to 285°C @ 5°C/min. (hold 30 min.)Inj. & det. temp.: 285°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.2cc/min.)FID sensitivity: 256 x 10-11 AFSSplit ratio: 50:1

4

15

16

24

Crude Oil, Arabian LightRtx®-1

min. 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68

2221201918

1617

1514

1312

1110

9

8

7

6

5

4

3

21

GC_PC00232

1. heptane2. octane3. nonane4. decane5. undecane6. dodecane7. tridecane8. tetradecane9. pentadecane

10. hexadecane11. heptadecane

12. octadecane13. nonadecane14. eicosane15. heneicosane16. docosane17. tricosane18. tetracosane19. pentacosane20. hexacosane21. heptacosane22. octacosane

30m, 0.53mm ID, 3.0µm Rtx®-1 (cat.# 10185)0.6µL split injection of an Arabian light crude oil.Oven temp.: 40°C to 285°C @ 5°C/min. (hold 15 min.)Inj. & det. temp.: 285°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.2cc/min.)FID sensitivity: 128 x 10-11 AFSSplit ratio: 50:1

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682 Hydrocarbons


GC_PC00594

Crude Oil, Pennsylvania Light MXT®-1HT Sim Dist

MXT®-1HT Sim Dist 5m, 0.53mm ID, 0.10µm (cat.# 70100)Oven temp: 50°C, 10°C/minute, 430°C, 6 min. final holdInj. temp.: oven trackingDet. temp: 430°CCarrier gas: helium – 1.8 psi, 14mL/min.Inj. volume: 0.2µLStd. conc.: approx. 2% in carbon disulfide

Lubricant (Synthetic)Stabilwax®

30m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10655)1.0µL split injection of a synthetic lubricantOven temp.: 100°C to 175°C @ 5°C/min., to 225°C

@ 15°C/min. (hold 10 min.)Inj. / det. temp.: 250°C / 275°CCarrier gas: heliumLinear velocity: 20cm/sec. (flow rate: 2.6cc/min.)FID sensitivity: 8 x 10-11 AFSSplit vent: 40:1

min. 4 8 12 16 20 24 28

10

9

76

5

1 2 3

84

1. C142. C153. C164. C10 OH5. C12 ester6. BHT7. C12 OH8. C14 ester9. C14 OH

10. C16 OH

GC_PC00219

Petroleum WaxMXT®-1

0 min. 36

1

2

3

4

56

7

8

9

GC_PC00218

30m, 0.28mm ID, 0.25µm MXT®-1 (cat.# 70124)1.0µL cool on-column injection of petroleum wax.Conc.: 0.1% w/w in cyclohexaneOven temp.: 50°C to 400°C @ 10°C/min. (hold 10 min.)Det. temp.: 400°CCarrier gas: hydrogenLinear velocity: 60cm/sec. set @ 50°CFID sensitivity: 64 x 10-11 AFS

1. hexadecane - C162. eicosane - C203. tetracosane - C244. octacosane - C285. dotricontane - C326. hexatricontane - C367. tetracontane - C408. tetratetracontane - C449. pentacontane - C50

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Carnauba WaxRtx®-1

15m, 0.53mm ID, 0.10µm Rtx®-1 (cat.# 10107)0.1µL direct injection of carnauba waxConc.: approximately 80mg/mLOven temp.: 75°C (hold 2 min.) to 340°C @

15°C/min. (hold 10 min.)Inj. & det. temp.: 340°CCarrier gas: hydrogenLinear velocity: 80cm/sec.FID sensitivity: 32 x 10-11 AFS

min. 4 8 12 16 20 24 28 32 36

GC_PC00220

1. C242. C283. C324. C365. C406. C447. C488. C529. C56

Petroleum WaxRtx®-1

1

23 4

5 6

7

8

9

min. 4 8 12 16 20 24 28 32 36 40GC_PC00221

30m, 0.53mm ID, 0.25µm Rtx®-1 (cat.# 10125)1.5µL direct injection, Uniliner® linerOven temp.: 200°C to 340°C @ 10°C/min.Inj. & det. temp.: 340°CCarrier gas: hydrogenLinear velocity: 80cm/sec. (flow rate: 10cc/min.)FID sensitivity: 128 x 10-11 AFS

Petroleum Wax (High Temperature)MXT®-1

min. 0 44GC_PC00223

15m, 0.53mm ID, 0.20µm MXT®-1 (cat.# 70108)1.0µL on-column injection of neat parafin wax in methylene chloride.Oven temp.: 100°C to 400°C @ 10°C/min. (hold 20 min.).Inj. / det. temp.: 103°C / 430°CInjector type: HP cool on-column pressure programmableCarrier gas: hydrogenLinear velocity: 30cm/sec.FID sensitivity: 2.56 x 10-11 AFS

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684 Hydrocarbons


Hydrocarbons, C44-C100 MXT®-1HT Sim Dist

MXT®-1HT Sim Dist 5m, 0.53mm ID, 0.10µm (cat.# 70100)Sample: Polywax® 1000 (cat.# 36227)Solvent: carbon disulfideSample size: 0.2µLInstrument: Agilent 5890 GC w/GC Racer*Injector: on-column (track oven)Carrier gas: hydrogen, constant pressure (1.0psi)Detector: FID/430°COven temp.: 40°C to 430°C @ 60°C/min. (hold 30 min.)

*Manufactured by Zip Scientific, distributed by Restek Corporation.

GC_PC00543

Program your Agilent 5890 or6890 GC to 430°C at60°C/min., using a GC Racer!See page 149 for more infor-mation.

10 20 30 40 min.00

10

20

30

40

50

60

70

pA

5m, 0.53mm ID, 0.10µm MXT®-1HT Sim Dist(cat.# 70100)Oven temp.: 40°C to 430°C @ 10°C/min. (hold 30 min.)Injector: on-column (track oven)Carrier gas: helium (constant pressure)Head pressure: 1.0psiLinear velocity: 60cm/sec.Flow rate: 7.8mL/min.Det.: FID @ 430°CMake-up gas flow: 40cc/min.

430°C

Bleed ProfileMXT®-1HT Sim Dist

GC_PC00527 20 30 40 50 min.

C30 C

40 C44

C50

C60

C70

C80

C90

C10

0

C11

0

5m, 0.53mm ID, 0.10µm MXT®-1HT Sim Dist (cat.# 70100)0.2µL Polywax® 1000 standard (cat.# 36227)Solvent: carbon disulfideOven temp.: 40°C to 430°C @ 10°C/min. (hold 30 min.)Injector: on-column (track oven)Carrier gas: helium (constant pressure)Head pressure: 1.0psiLinear velocity: 60cm/sec.Flow rate: 7.8mL/min.Det.: FID @ 430°CMake-up gas flow: 40cc/min.


GC_PC00530

tech tip

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S685Hydrocarbons (Simulated Distillation)


Hydrocarbons, C44-C100MXT®-1HT Sim Dist(fast GC)

5m, 0.53mm ID, 0.10µm MXT®-1HT Sim Dist (cat.# 70100)0.2µL injection of Polywax® 1000Solvent: carbon disulfideOven temp.: 40°C to 430°C @ 60°C/min. (hold 25 min.)Injector: on-column (track oven)Carrier gas: helium (constant pressure)Head pressure: 1.0psiLinear velocity: 60cm/sec.Flow rate: 7.8mL/min.Det.: FID/430°CMake up gas flow: 40cc/min.

GC_PC00531 5 10 15 20 25 min.

C9

C10

C11

C14

C12

C16

C18

C20

C24

C28

C32

C36

C40

C44

5m, 0.53mm ID, 0.10µm MXT®-1HT Sim Dist (cat.# 70100)0.2µL hydrocarbon standard (cat.# 31222)Solvent: carbon disulfideOven temp.: 40°C to 430°C @ 10°C/min. (hold 30 min.)Injector: on-column (track oven)Carrier gas: helium (constant pressure)Head pressure: 1.0psiLinear velocity: 60cm/sec.Flow rate: 7.8mL/min.Det.: FID @ 430°CMake-up gas flow: 40cc/min.

GC_PC00529


Sim Dist, High-TemperatureMXT®-1 Sim Dist(calibration)

3

2

5

4

67

8

9

10

11 12

13 1415

1617

min. 4 8 12 16 20 24

1. C62. C73. C84. C95. C106. C117. C128. C149. C16

10. C1811. C2012. C2413. C2814. C3215. C3616. C4017. C44

1

GC_PC00224

6m, 0.53mm ID, 0.15µm MXT®-1 Sim Dist (cat.# 70101)Wet needle on-column injection of ASTM D2887 standard.

Oven temp.: -12°C to 430°C @ 20°C/min. Inj. & det. temp.: -17°C to 433°C/430°CCarrier gas: hydrogenLinear velocity: 40cm/sec. FID sensitivity: 128 x 10-11 AFS

Sim Dist, High-Temperature MXT®-500 Sim Dist

6m, 0.53mm ID, 0.15µm MXT®-500 Sim Dist (cat.# 70104)cold on-column injection of Polywax® 655 in CS2

Oven temp.: 40°C to 430°C @ 6°C/min.Carrier gas: heliumLinear velocity: 40cm/sec.FID sensitivity: 2 x 10-11AFS

20 24 28 32 36 40 44 48 52 56 60 64 68min.

GC_PC00225

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686 Hydrocarbons (Simulated Distillation)


Sim Dist, ASTM D-2887 (C5 to C44 Standard) Rtx®-2887 Column

1. pentane2. hexane3. heptane4. octane5. nonane6. decane7. undecane8. dodecane9. tetradecane

10. hexadecane11. octadecane12. eicosane13. tetracosane14. octacosane15. dotricontane16. hexatricontane17. tetracontane18. tetratetracontane

min. 5 10 15 20

18171615141312

11

10

9

8

1

2 3 4 5 6 7

GC_PC00226

10m, 0.53mm ID, 2.65µm Rtx®-2887 (cat.# 10199)1.0µL direct injection of a 0.1 to 0.01 wt % hydrocarbon standard in carbon disulfideOven temp.: 35°C to 360°C @ 15°C/min. (hold 5 min.)Inj. & det. temp.: 360°CCarrier gas: nitrogenLinear velocity: 112cm/sec. (15mL/min.)

Sim Dist, ASTM D-2887 (Reference Gas Oil #1)Rtx®-2887

min. 5 10 15 20GC_PC00227

10m, 0.53mm ID, 2.65µm Rtx®-2887 (cat.# 70199)1.0µL direct injection of a 0.1 to 0.01 wt % hydrocarbon standard in carbon disulfide.Oven temp.: 35°C to 360°C @ 15°C/min. (hold 5 min.)Inj. & det. temp.: 360°CCarrier gas: nitrogenLinear velocity: 112cm/sec. (15mL/min.)

1. pentane2. hexane3. heptane4. octane5. nonane6. decane7. undecane8. dodecane9. tetradecane

10. hexadecane11. octadecane12. eicosane13. tetracosane14. octacosane15. dotricontane16. hexatricontane17. tetracontane18. tetratetracontane

Sim Dist, ASTM D-2887Rtx®-1 SimDist 2887

1 2 3 4 5 6 7 8 9 10 11

1213

14 15 16 17 18

min. 0 10 20 30

GC_PC00228

25" x 1/8" x 2mm ID Rtx®-1 SimDist 2887, SilcoSmooth™ stainless steel (cat.# 80000)1.0µL direct injection, 1–12% (w/w) each componentOven temp.: 35°C to 350°C @ 10°C/min. (hold 5 min.)Inj. & det. temp.: 350°CCarrier gas: helium @ 25mL/min.FID sensitivity: 256 x 10-11 AFS

cat.# 31674 (1% each listed analyte in CS2) and cat.# 31675 (5% each, neat) meet requirements of ASTM D2887-01.

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S687Hydrocarbons (Simulated Distillation)


Sim Dist, ASTM D-3710Rtx®-1 SimDist 2887(calibration)

25" x 1/8" OD x 2mm ID Rtx®-1 SimDist 2887, SilcoSmooth™ stainless steel (cat.# 80000)D-3710 Calibration Mix, C3, C4, C16, & C17 addedOven temp: -30°C to 250°C @ 10°C/min.Inj./det. temp.: 250°C / 300°CCarrier gas: helium @ 25mL/min.FID sensitivity: 256 x 10-11 AFS

1. n-propane2. 2-methyl propane3. n-butane4. 2-methylbutane5. n-pentane6. 2-methylpentane7. n-hexane8. 2,4-dimethylpentane9. n-heptane

10. toluene11. n-octane12. p-xylene13. n-propylbenzene14. n-decane15. n-butylbenzene16. n-dodecane17. n-tridecane18. n-tetradecane19. n-pentadecane20. n-hexadecane21. n-heptadecane2

3

1

min. 0 5 10 15 20

4 5 6 7 8 9 10 11 12 13

14

15

16

17 18 19 20 21

GC_PC00229

Sim Dist, ASTM D-3710 (Gasoline)Rtx®-1 SimDist 2887

25" x 1/8" OD x 2mm ID Rtx®-1 SimDist 2887, SilcoSmooth™ stainless steel (cat.# 80000)1.0µL direct injection of unleaded gasoline (ASTM D-2887)Oven temp: -30°C to 250°C @ 10°C/min.Inj./det. temp.: 250°C/300°CCarrier gas: helium @ 25mL/min.FID sensitivity: 256 x 10-11 AFS

1. n-propane2. 2-methyl propane3. n-butane4. 2-methylbutane5. n-pentane6. 2-methylpentane7. n-hexane8. 2,4-dimethylpentane9. n-heptane

10. toluene11. p-xylene12. n-propylbenzene13. n-decane14. n-butylbenzene

min. 0 5 10 15

4

6

5

2

1

7

89

10 11

12

1413

GC_PC00230

3

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688 Sulfur Compounds


min. 4 8 12 16 20 24 28

1 23

4

5

6

7

8

1. thiophene2. 2-methylthiophene3. 3-methylthiophene4. 2-ethylthiophene5. alkylthiophenes6. benzothiophene7. methylbenzothiophenes8. dimethylbenzothiophenes

Sulfur in GasolineRtx®-1

GC_PC00198

30m, 0.32mm ID, 4.0µm Rtx®-1 (cat.# 10198)1.0µL split injection of gasoline containing 300ppm total sulfur


Inj. & det. temp.: 275°CDet.: SCD Carrier gas: heliumLinear velocity: 70cm/sec. (flow rate: 2.5mL/min.)Split ratio: 10:1

min. 0 10 20

1 2

3

45

1. hydrogen sulfide2. thiophene3. 2-methylthiophene4. 3-methylthiophene5. 2-ethylthiophene

Sulfur in Naphtha by AED Rtx®-1

GC_PC00199

30m, 0.32mm ID, 4.0µm Rtx®-1 (cat.# 10198 )1.0µL split injection of naphtha containing 500ppm total sulfur

Oven temp.: 35°C to 275°C @ 10°C/min. (hold 5 min.).Inj. & det. temp.: 275°CDet.: AED (181nm)Carrier gas: heliumLinear velocity: 24cm/sec. (flow rate: 0.8mL/min.)Split ratio: 10:1

ShinCarbon ST 100/120 mesh (cat.# 19808)2 meter x 1mm ID Silcosteel® Micropacked columnSample: 100ppm sulfur dioxide in heliumInj.: 10µLInj. temp.: 100°CCarrier gas: heliumFlow rate: 10mL/min.Oven temp.: 150°CDet.: HID @ 200°C

1. sulfur dioxide

Sulfur DioxideShinCarbon ST(micropacked)

GC_PC00674

Sulfur CompoundsRtx®-1

60m, 0.53mm ID, 7.0µm Rtx®-1 (cat.# 10193)100µL direct injection of sulfur compounds

Oven temp.: 50°C to 200°C @ 15°C/min.Inj. / det. temp.: 50°C / 230°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 50°CDet.: FPD

min. 0 4 8 12

1

23

4

5 6

7

8 9

10

11

12 13

1415

GC_PC00200

1. hydrogen sulfide2. sulfur dioxide & carbonyl sulfide3. methyl mercaptan4. ethyl mercaptan5. carbon disulfide6. dimethyl sulfide7. 2-propyl mercaptan8. allyl mercaptan9. 1-propyl mercaptan

10. ethyl sulfide11. butyl mercaptan12. dimethyl disulfide13. allyl sulfide14. propyl sulfide15. butyl sulfide &

allyl disulfide

free literatureFor more information, request

lliitt.. ccaatt..## 5599551199AA

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S689Sulfur Compounds


1m, 0.75mm ID Rt-XLSulfur™ micropacked column (cat.# 19806)Conc.: 50ppb eachOven temp.: 60°C to 230°C @ 15°C/min. Carrier gas: heliumFlow rate: 9mL/min.Det.: SCD/FID

Sulfur standards courtesy of DCG Partnership 1 Ltd., Pearland, TX.

Sulfur CompoundsRt-XLSulfur™

ssuullffuurrss1. hydrogen sulfide2. carbonyl sulfide3. methyl mercaptan4. ethyl mercaptan5. dimethyl sulfide6. dimethyl disulfide

hhyyddrrooccaarrbboonnssA. methaneB. ethaneC. propyleneD. propaneE. isobutaneF. butaneG. isopentaneH. pentaneI. hexane

1

23

4/5

6

min. 2 4 6 8 10GC_PC00436

A B

C

D

E

F

GH

I

Sulfur Impurities in EthyleneRt-Sulfur™

1. hydrogen sulfide2. carbonyl sulfide

1 2

ethylene

min. 5 10 15 20

GC_PC00202

2m x 1/8" x 2mm Rt-Sulfur™ (cat.# 80252)1cc injection of sulfur compounds

Oven temp.: 40°C to 100°C @ 5°C/min.Det.: FPD (sensitivity: range 1)Carrier gas: heliumFlow rate: 20mL/min.

Sulfur Impurities in PropyleneRt-Sulfur™

1. carbonyl sulfide2. propylene

GC_PC00203

min. 5 10 15 20

1

2

2m x 1/8" x 2mm Rt-Sulfur™ (cat.# 80252)400µL injection

Oven temp.: 60°C to 100°C @ 5°C/min. (hold 15 min.)Det.: FPD (sensitivity: range 3)Carrier gas: heliumFlow rate: 20mL/min.


www.restek.com

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690 Antidepressants; Antihistamines


1. amitriptyline2. trimipramine3. imipramine4. nortriptyline5. doxepin6. desipramine7. maprotyline8. clomipramine

1

2

3

4

5

6

78

min. 1 2 3 4 5 6

Underivatized Antidepressants (Basic Drugs)Rtx®-1701

15m, 0.25mm ID, 0.25µm Rtx®-1701 (cat.# 12020)1.0µL split injection of antidepressantsConc.: 25ng/component

Oven temp.: 225°CInj. / det. temp.: 250°C / 260°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 225°CFID sensitivity: 2.56 x 10-10 AFSSplit ratio: 40:1

GC_PH00264

Underivatized Antihistamines (Basic Drugs)Rtx®-5 Amine

1. phenylpropanolamine2. ephedrine3. pseudoephedrine4. pheniramine5. diphenhydramine6. doxylamine7. phenyltoloxamine8. methapyrilene9. chlorpheniramine

10. brompheniramine11. triprolidine

30m, 0.32mm ID, 1.0µm Rtx®-5 Amine (cat.# 12354)1.0µL split injection of antihistamines.Conc.: 1000ng/µL


Inj. & det. temp.: 305°CCarrier gas: hydrogenLinear velocity: 43cm/sec. set @ 130°CFID sensitivity: 6.4 x 10-11 AFSSplit ratio: 50:1

GC_PH00247min. 5 10 15 20 25

1

2

11

10

9

8

7

6

5

4

3

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L691Antiepileptics


Underivatized AntiepilepticsRtx®-20

15m, 0.32mm ID, 0.50µm Rtx®-20 (cat.# 10336)1.0µL split injection of antiepileptic drugsConc.: 1000ng/µL

Oven temp.: 150°C to 280°C @7°C/min. (hold 5 min.)

Inj. / det. temp.: 250°C / 270°CDet.: FIDCarrier gas: heliumLinear velocity: 30cm/sec. set @ 150°CFID sensitivity: 1.28 x 10-10 AFSSplit ratio: 30:1

1

min. 5 10 15 20

2

3 4

5

6

7 8 9

10

11

12

GC_PH00265

Underivatized AntiepilepticsRtx®-1701

1. valproic acid2. ethosuximide3. methsuximide4. phensuximide5. desmethyl methsuximide6. mephenytoin7. ethotoin8. PEMA9. phenobarbital

10. primidone11. carbamazepine12. diphenylhydantoin

15m, 0.32mm ID, 0.50µm Rtx®-1701 (cat.# 12036)1.0µL split injection of antiepileptic drugsConc.: 1000ng/µL


Inj. / det. temp.: 250°C / 270°CDet.: FIDCarrier gas: heliumLinear velocity: 30cm/sec. set @ 150°CFID sensitivity: 1.28 x 10-10 AFSSplit ratio: 30:1

GC_PH00266

1

min. 5 10 15 20

23

4

5

6

7 8

910

11

12

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692 Barbiturates


6 75

30m, 0.53mm ID, 1.0µm Rtx®-35 (cat.# 10455)1.0µL splitless injection of acidic/neutral drugsConc.: 50µg/mL

Oven temp.: 100°C to 280°C @ 10°C/min. (hold 5 min.)Inj. & det. temp.: 250°CCarrier gas: heliumLinear velocity: 40cm/sec. set @ 100°CFID sensitivity: 5.12 x 10-10 AFSSplitless hold time: 0.5 min.

Underivatized Barbiturates (Acidic/Neutral Drugs)Rtx®-35

1

2

3

4

8

9

11

10 12

13

16

15

14

min. 0 5 10 15 20GC_PH00262

1. ethosuximide2. barbital3. methyprylon4. aprobarbital5. butalbital6. amobarbital7. pentobarbital8. secobarbital9. meprobamate

10. carisoprodal11. glutethimide12. phenobarbital13. methaqualone14. primidone15. carbamazepine16. diphenylhydantoin

15m, 0.53mm ID, 0.50µm Rtx®-1701 (cat.# 12037)1.0µL splitless injection of acidic/neutral drugs50µg/mL concentration

Oven temp.: 100°C to 280°C @ 7°C/min.Inj. / det. temp.: 250°C / 275°CCarrier gas: heliumLinear velocity: 40cm/sec. set @ 100°CFID sensitivity: 5.12 x 10-10 AFSSplitless hold time: 0.5 min.

6 7


1 3

2

45

8

11

9

15

1012

13

14

16

min. 0 5 10 15 20GC_PH00263

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L693


Barbiturates; Basic Drugs


30m, 0.32mm ID, 0.50µm Rtx®-35 (cat.# 10439)1.0µL split injection of barbiturates


Inj. & det. temp.: 300°CCarrier gas: heliumLinear velocity: 35cm/sec. set @ 210°CFID sensitivity: 5.12 x 10-10 AFSSplit vent: 30:1

1. barbital2. aprobarbital3. butalbital4. amobarbital5. talbutal6. pentobarbital7. methohexital8. secobarbital9. thiopental

10. hexobarbital11. mephobarbital12. phenobarbital

GC_PH00267

12

11

10

9

8

7

6

4

5

1 2

3

min. 2 4 6 8 10

Separation optimizedusing Pro ezGC™


7

30m, 0.25mm ID, 0.25µm Rtx®-200 (cat.# 15023)1.0µL split injection of a basic drug sample (1mg/mL)Conc.: 1000ng/µL

Oven temp.: 100°C to 325°C @ 4°C/min. (hold 10 min.)Inj. / det. temp.: 250°C / 320°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 100°CFID sensitivity: 1.28 x 10-10 AFSSplit ratio: 50:1

1

2

4

6

8

10

11

3,5

14

19

17

18

22

23

21

20,25

2430

28,29

27

37

34

33 3638

35

31,32

26

16

15

13

12

9

min. 10 20 30 40 50 60

GC_PH00237

Underivatized Mixed Basic Drugs Rtx®-200 1. nicotine

2. benzocaine3. cotinine4. meperidine5. caffeine6. benzphetamine7. ketamine8. diphenhydramine9. lidocaine

10. phenyltoloxamine11. tripelennamine12. phenothiazine13. dextromethorphan14. methadone15. amitriptyline16. trimipramine17. tetracaine18. pyrilamine19. medazepam20. bupivacaine21. scopolamine22. codeine23. morphine24. diazepam25. chlorpromazine26. temazepam27. flunitrazepam28. bromazepam29. prazepam30. acetopromazine31. flurazepam32. papaverine33. clonazepam34. haloperidol35. alprazolam36. triazolam37. thioridazine38. trazodone

Analysis on Rtx®-5 and Rtx®-35 columns: page 694.

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694 Basic Drugs


1. nicotine2. benzocaine3. cotinine4. meperidine5. caffeine6. benzphetamine7. ketamine8. diphenhydramine9. lidocaine

10. phenyltoloxamine11. tripelennamine12. phenothiazine13. dextromethorphan14. methadone15. amitriptyline16. trimipramine17. tetracaine18. pyrilamine19. medazepam20. bupivacaine21. scopolamine22. codeine23. morphine24. diazepam25. chlorpromazine26. temazepam27. flunitrazepam28. bromazepam29. prazepam30. acetopromazine31. flurazepam32. papaverine33. clonazepam34. haloperidol35. alprazolam36. triazolam37. thioridazine38. trazodone

min. 10 20 30 40 50 60

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

2120

22

24

25

26

28

27

29

30

31

32

23

33

35

36

37

34

1617

18

19

GC_PH00235

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)1.0µL split injection of a basic drug sample.Conc.: 1000ng/µL


38

Underivatized Mixed Basic DrugsRtx®-5

Underivatized Mixed Basic DrugsRtx®-35

min. 10 20 30 40 50 60

1

2

3

4

6

8

9

107

5

11

3836

3735

33

3432

30

27

29

2326

24

25

22

21

19

18,20

1514

12 17

16

13

GC_PH00236

30m, 0.25mm ID, 0.25µm Rtx®-35 (cat.# 10423)1.0µL split injection of a basic drug sampleConc.: 1000ng/µL


Inj. / det. temp.: 250°C / 320°CCarrier gas: heliumLinear velocity: 30cm/sec. set @ 100°CFID sensitivity: 1.28 x 10-10 AFSSplit ratio: 50:1

3128

Analysis on Rtx®-200 column: page 693

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L695


Basic Drugs

Underivatized Phenothiazines (Basic Drugs)Rtx®-5

1

9

87

5,64

3

2

15m, 0.32mm ID, 0.50µm Rtx®-5 (cat.# 10236)1.0µL split injection of phenothiazinesConc.: 2000ng/µL


min. 5 10 15 20 25

10

GC_PH00269

1. phenothiazine2. promethazine3. trimeprazine4. promazine5. chlorprothixene6. chlorpromazine7. trifluoperazine8. acetopromazine9. prochlorperazine

10. thioridazine

Separation optimizedusing Pro ezGC™


min. 2 4 6 8 10

1

2

3

4

5,6

7

89

10

11

12

13

15m, 0.32mm ID, 0.25µm Rtx®-200 (cat.# 15021)1.0µL split injection of benzodiazepinesConc.: 15ng/component


GC_PH00268

Underivatized Benzodiazepines (Basic Drugs)Rtx®-200

1. medazepam2. lorazepam3. diazepam4. desmethyl diazepam5. bromazepam6. prazepam7. temazepam8. flurazepam9. clobazam

10. flunitrazepam11. clonazepam12. alprazolam13. triazolam

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696 Basic Drugs


1. phenylpropanolamine2. phenylephrine3. guaifenesin4. pheniramine5. phenyltoloxamine6. chlorpheniramine7. brompheniramine8. dextromethorphan9. pyrilamine

10. codeine11. hydrocodone

30m, 0.53mm ID, 1.0µm Rtx®-5 Amine (cat.# 12355)

Oven temp.: 175°C to 280°C @ 10°/min. (hold 5.5 min.)Inj./det. temp.: 250°C/280°CCarrier gas: heliumLinear velocity: 40cm/sec.Sample size: 1µLSplit vent flow: 88mL/min.Split ratio: 20:1

1

2

3

4

5

6

7

8

9

10

11

min. 2 4 6 8 10 12 14 16

Underivatized Cold Medications (Basic Drugs)Rtx®-5 Amine

GC_PH00469

Rtx®-35 Amine 30m, 0.25mm ID, 0.50µm (cat.# 11338)1.0µL split injection of underivatized cold medicinesConc.: 1000ng/µLInj. temp: 250°CCarrier gas: heliumLinear velocity: 30cm/sec.Oven temp.: 250°C to 300°C @ 7°C/min. (hold 7 min.)Det.: MSDScan range: 40–450amuIonization: EIMode: scan

Underivatized Cold Medications (Basic Drugs)Rtx®-35 Amine

1. guaifenesin2. pheniramine3. phenyltoloxamine4. chlorpheniramine5. brompheniramine6. dextromethorphan7. pyrilamine8. triprolidine9. codeine

10. hydrocodone

GC_PH00575

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L697Chiral Analyses


30m, 0.32mm ID, 0.25µm Rt-βDEXsm™ (cat.# 13104)On-column injectionConc.: 125ng each enantiomer

Oven temp.: 175°C to 200°C @ 2°C/min. Inj./det. temp.: 200°C/230°CCarrier gas: heliumLinear velocity: 60cm/sec.Det.: GC-FID Split ratio: 13:1 using cup splitter liner (cat.# 20709)

min. 7 8

(+/-

)ibup

rofe

n

Underivatized IbuprofenRt-βDEXsm™

GC_PH00242

30m, 0.32mm ID, 0.25µm Rt-βDEXcst™ (cat.# 13102) On-column injectionConc.: approximately 15ng each enantiomer

Oven temp.: 200°C to 230°C @ 1°C/min.Inj./det. temp.: 200°C/230°C Carrier gas: hydrogenLinear velocity: 80cm/sec.Detector type: GC/FIDSplit ratio: 13:1 using cup splitter liner (cat.# 20709)

min. 20 25

(+/-

)thal

idom

ide

Underivatized ThalidomideRt-βDEXcst™

GC_PH00243

a plus 1 storyGinny and her teammates completed a system upgrade that has improved the efficiency and qualityof procedures and processing. Members of the team also dedicated extensive time to preparing andtesting before, during, and after the upgrade with some members working nearly two weeksstraight, at all hours of the day, including Friday nights, Saturdays, and Sundays.

GGiinnnnyy EEsshh,, BBrriiaann CCoonnddoo,, KKaarreenn UUrrbbaanniikk,, SStteevvee KKrreeiisseerr,, CChhrriissttiiaall MMoorrggaann,, LLiizz HHaammiillttoonn,, DDaanniieellllee HHiicckkss,, JJiimm SStteeyyeerrss,, SSeeaann NNoollaann

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698 Chiral Analyses


Amphetamine and Methamphetamine (TFAA Derivatives)Rt-βDEXcst™

min. 10 12 14 16 18 20 22 24

30m, 0.25mm ID, 0.25µm Rt-βDEXcst™ (cat.# 13103)Oven temp.: 120°C (hold 1 min.) to

175°C @ 1.5°C/min.Carrier gas: heliumLinear velocity: 25cm/sec. set @ 120°C Det.: MSD set @ 220°C.

GC_PH00246

1. (+)methamphetamine - TFAA2. (-)methamphetamine - TFAA3. (+)amphetamine - TFAA4. (-)amphetamine - TFAA

1

2

3 4

Fenafluramine (TFAA Derivative)Rt-βDEXcst™

min. 15 20 25 30 35 40

30m, 0.32mm ID, 0.25µm Rt-βDEXcst™ (cat.# 13102) On-column injection, 25ng/enantiomer


Carrier gas: hydrogenLinear velocity: 80cm/sec. set @ 60°CDet.: FID set @ 220°C

1. (+)-fenfluramine - TFAA2. (-)-fenfluramine - TFAA3. (+)-norfenfluramine - TFAA4. (-)-norfenfluramine - TFAA

1

2

3

4

GC_PH00244

Underivatized BarbituratesRt-βDEXcst™

min. 10 20 30 40 50

30m, 0.32mm ID, 0.25µm Rt-βDEXcst™ (cat.# 13102) On-column conc.: 25ng/enantiomer


Carrier gas: hydrogenLinear velocity: 80cm/sec. set @ 60°CDet.: FID set @ 220°C

1. (+/-)-hexobarbital2. (+/-)-mephobarbital

12

GC_PH00245

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L699


Organic Volatile Impurities / Residual Solvents

Organic Volatile ImpuritiesRtx®-5 1. methanol

2. ethanol3. acetone4. acetonitrile5. isopropanol6. ether7. tert-butanol8. methylene chloride9. n-propanol

10. methyl ethyl ketone11. sec-butanol12. hexane13. ethyl acetate14. chloroform15. tetrahydrofuran16. benzene17. cyclohexane18. heptane19. trichloroethylene20. 1,4-dioxane21. pyridine22. toluene23. dimethylformamide

30m, 0.53mm ID, 5.0µm Rtx®-5 (cat.# 10279)0.5µL split injection of residual solvents

Oven temp.: 35°C (hold 7 min.) to 240°C @ 15°C/min.Inj. / det. temp.: 180°C / 260°CCarrier gas: heliumLinear velocity: 25cm/sec. set @ 35°CFID sensitivity: 2.56 x 10-10 AFSSplit ratio: 20:1

1

2 3,4

5

67

8

9

11,12

13

14

15

18

10

21

22

19

23

min. 2 4 6 8 10 12 14 16

16,17

20

GC_PH00256

Organic Volatile ImpuritiesRtx®-1301

GC_PH00257

1. methanol2. ethanol3. ether4. acetone5. isopropanol6. acetonitrile7. methylene chloride8. tert-butanol9. hexane

10. n-propanol11. methyl ethyl ketone12. ethyl acetate13. tetrahydrofuran14. sec-butanol15. chloroform16. cyclohexane17. benzene18. heptane19. trichloroethylene20. 1,4-dioxane21. pyridine22. toluene23. dimethylformamide

30m, 0.53mm ID, 3.0µm Rtx®-1301 (cat.# 16085)0.5µL split injection of residual solvents.

Oven temp.: 35°C (hold 8 min.) to 240°C @ 20°C/min.Inj. / det. temp.: 180°C / 260°CCarrier gas: heliumLinear velocity: 25cm/sec. set @ 35°CFID sensitivity: 1.28 x 10-10 AFSSplit ratio: 30:1

12

34

56

7

8 910

11

12

13 14

15

16

17

18

1920

21

22

23

min. 2 4 6 8 10 12 14 16 18

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L700


Organic Volatile Impurities(USP 467)Rtx®-G27

30m, 0.53mm ID, 5.0µm Rtx®-G27 with 5m phenylmethyl Integra-Guard™ guard column (cat.# 10279-126)Headspace injection of USP 467 Calibration Mix (cat.# 36004) prepared at limit test concentrations

Oven temp.: 35°C (hold 5 min.) to 175°C @ 8°C/min., to 260°C @ 35°C/min.

Inj./det. temp.: 180°C/260°CFID sensitivity: 1 x 10-11 AFS Carrier gas: helium, 35cm/sec. set @ 35°C Split ratio: 2:1

4

1

2

3

5

1. methylene chloride2. chloroform3. benzene4. trichloroethylene5. 1,4-dioxane

min. 2 4 6 8 10 12 14 16 18

GC_PH00258

Organic Volatile Impurities(USP 467)Rtx®-G43

30m, 0.53mm ID, 3.0µm Rtx®-G43 with 5m phenylmethyl Integra-Guard™ guard column (cat.# 16085-126) Headspace injection of USP 467 Calibration Mix (cat.# 36004) prepared at limit test concentrations


Inj./det. temp.: 180°C/260°C FID sensitivity: 1.25 x 10-11 AFS Carrier gas: helium, 35cm/sec. set @ 35°C Split ratio: 2:1

1

2

3

4

5

min. 2 4 6 8 10 12 14 16GC_PH00259

Organic Volatile ImpuritiesRtx®-G27

2

45

1

min. 2 4 6 8 10 12 14 16 18 20 22

8,6,7 9

12,10

1113

14

1516

18,17,22

19

20

21

23

24

25

3

30m, 0.53mm ID, 5.0µm Rtx®-G27 with 5m phenylmethyl Integra-Guard™ guard column (cat.# 10279-126)

Headspace injection of common solvents for pharmaceutical processing. Prepared to equal about 500ppm inthe bulk pharmaceutical. Samples shaken and heated at 90°C for 15 minutes, 1mL headspace injection.

Oven temp.: 35°C (hold 10 min.) to 100°C @ 5°C/min., to 240°C @ 25°C/min. (hold 5 min.)Inj./det. temp.: 220°C/240°C FID sensitivity: 1.05 x 10-11 AFS Carrier gas: helium, 35cm/sec. set @ 35°C Split ratio: 2:1

GC_PH00260

Organic Volatile ImpuritiesRtx®-G43

30m, 0.53mm ID, 3.0µm Rtx®-G43 with 5m phenylmethyl Integra-Guard™ guard column (cat.# 16085-126)

2

3,45

6

1 8

9

10

117

14

151612

18

19

20

21

17 23

22

1. ethylene oxide2. methanol3. ethanol4. diethyl ether5. 1,1-dichloroethene6. acetone7. isopropanol8. acetonitrile9. methylene chloride

10. n-hexane11. n-propanol12. methyl ethyl ketone13. ethyl acetate14. tetrahydrofuran15. chloroform16. 1,1,1-trichloroethane17. carbon tetrachloride18. benzene

19. 1,2-dichloroethane20. heptane21. trichloroethylene22. n-butanol23. 1,4-dioxane24. pyridine25. toluene

min. 2 4 6 8 10 12 14 16 18

24

25

13

GC_PH00261

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L701Organic Volatile Impurities / Residual Solvents; Steroids


6

30m, 0.25mm ID, 0.10µm Rtx®-5 (cat.# 10208)0.5µL split injection of anabolic steroidsConc.: 1000ng/µL


1. 5-androstene-3β,17β-diol2. 17α-methyl-5-androstene-3β,17β-diol3. 5α-androstan-17β-ol-3-one4. 19-nortestosterone5. 17α-methylandrostan-17β-ol-3-one6. mesterolone7. testosterone8. 17α-methyltestosterone9. 1-dehydrotestosterone

10. 1-dehydro-17α-methyltestosterone11. bolasterone12. oxymethalone13. 19-nortestosterone-17-propionate14. testosterone propionate15. fluoxymesterone16. 4-chlorotestosterone-17-acetate17. testosterone-17β-cypionate18. 1-dehydrotestosterone benzoate19. 1-dehydrotestosterone undecylenate

Underivatized Steroids, Anabolic Rtx®-5

1

1918

17

2

3

5

7

8

9

10,11

12

1314

15

164

min. 2 4 6 8 10 12 14 16 18GC_PH00255

30m, 0.53mm ID x 3.0µm Rtx®-1301 (cat.# 16085)

Headspace injection of 28 Class 1 and 2 residual solvents for pharmaceutical processing. Prepared atthe regulatory limit concentration, using samples shaken and heated at 80°C for 15 minutes, 1mL headspace injection.

Oven temp.: 40°C (hold 20 min.) to 240°C @ 10°C/min. (hold 20 min.)Inj./det. temp.: 200°C/250°CFID sensitivity: 1.1 x 10-11 AFSCarrier gas: hydrogen @ 35cm/sec.Split ratio: 2:1

1. methanol2. 1,1-dichloroethene 3. acetonitrile4. methylene chloride (dichloromethane)5. hexane (C6)6. cis-1,2-dichloroethene7. nitromethane8. chloroform9. cyclohexane

10. 1,1,1-trichloroethane11. carbon tetrachloride12. benzene13. 1,2-dimethoxyethane14. 1,2-dichloroethane15. trichloroethylene (1,1,2-trichlorethene)16. methylcyclohexane17. 1,4-dioxane18. pyridine19. toluene 20. 2-hexanone21. chlorobenzene22. DMF23. ethylbenzene24. m-xylene25. p-xylene26. o-xylene27. N,N-dimethylacetamide28. 1,2,3,4-tetrahydronaphthalene

2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34

1 23

4

5

6

78

9,10

11 12 13,1415

16

17 18

19

20 21

22,23

24,25

26

27

28

GC_PH00553

European Pharmacopoeia Class 1 and Class 2 SolventsRtx®-1301

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702 Sympathomimetic Amines


Rtx®-35 Amine 30m, 0.25mm ID, 0.50µm (cat.# 11338)1.0µL split injection of underivatized sympathomimetic aminesConc. 1000ng/µLInj. temp: 250°CCarrier gas: heliumLinear velocity: 30cm/sec.Oven temp.: 150°C to 240°C @ 7°C/min.Det.: MSDScan range: 40–450amuIonization: EIMode: scan

Underivatized Sympathomimetic Amines (Basic Drugs)Rtx®-35 Amine

GC_PH00574

1. amphetamine2. methamphetamine3. N-ethylamphetamine4. p-methoxyamphetamine (PMA)5. p-methoxymethamphetamine (PMMA)6. methylenedioxyamphetamine (MDA)7. methylenedioxymethamphetamine (MDMA)8. methylenedioxyethylamphetamine (MDEA)9. HMMA

10. MBDB


www.restek.com

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L703Sympathomimetic Amines


Underivatized Sympathomimetic Amines (Basic Drugs)Rtx®-5 Amine

1

2

34

5

6

7 8

9

10

11,12

13

14

15

16

17

18

19

21

22

1. phenylethylamine2. amphetamine3. phentermine4. methamphetamine5. fenfluramine6. mephentermine7. cathinone8. phenylpropanolamine9. methcathinone

10. nicotine11. ephedrine12. pseudoephedrine13. phenmetrazine14. phendimetrazine15. methylenedioxyamphetamine16. diethylpropion17. methylenedioxymethamphetamine18. methylenedioxyethylamphetamine19. 4-methyl-2,5-dimethoxyamphetamine20. phenylephrine21. caffeine22. benzphetamine

30m, 0.25mm ID, 0.50µm Rtx®-5 Amine (cat.# 12338)split mode, split vent flow rate 45mL/min.Oven temp.: 100°C to 310°C @ 10°C/min.

Varian 3400 GC coupled with Varian Saturn 2000 MS detectorMass spectroscopy data collected using a scan range of 40 amu through 600 amu. Ionization performed in the EI Auto mode.

min. 5 7.5 10 12.5 15 17.5 20GC_PH00438

Underivatized Sympathomimetic Amines (Basic Drugs)Rtx®-200

min. 5 7.5 10 12.5 15 17.5

1014

3

1,2

16

4

8

11

9

7

5

12

13

22

15,20

19 17

18

21

30m, 0.25mm ID, 0.50µm Rtx®-200 (cat.# 15038)split mode, split vent flow rate 45mL/min.Oven temp.: 100°C to 310°C @ 10°C/min.

Varian 3400 GC coupled with Varian Saturn 2000 MS detectorMass spectroscopy data collected using a scan range of 40 amu through 600 amu. Ionization performed in the EI Auto mode.

GC_PH00439

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704 Sympathomimetic Amines


Sympathomimetic Amines (TFAA Derivatives) (Basic Drugs)Rtx®-5

109

8

7

6

5

4

1

2,3

min. 4 6 8 10 12 14 16 18GC_PH00251

1. amphetamine - TFAA2. phentermine - TFAA3. phenylpropanolamine - TFAA4. ephedrine - TFAA5. methamphetamine - TFAA6. fenfluramine - TFAA7. pseudoephedrine - TFAA8. methylenedioxyamphetamine - TFAA9. methylenedioxymethamphetamine - TFAA

10. methylphenidate - TFAA

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)1.0µL splitless injection of derivatized sympathomimetic aminesConc.: approximately 2.5ng/µL


Inj. temp.: 225°CInterface temp.: 275°CDet.: MSDIonization: EICarrier gas: heliumLinear velocity: 20cm/sec. set @ 100°CSplitless hold time: 1 min.

Sympathomimetic Amines (HFBA Derivatives) (Basic Drugs)Rtx®-5

1

2

3

4

5

6

7

8

9

10

min. 4 6 8 10 12 14 16 18GC_PH00252

1. amphetamine - HFBA2. phentermine - HFBA3. phenylpropanolamine - HFBA4. ephedrine - HFBA5. methamphetamine - HFBA6. fenfluramine - HFBA7. pseudoephedrine - HFBA8. methylenedioxyamphetamine - HFBA9. methylenedioxymethamphetamine - HFBA

10. methylphenidate - HFBA

30m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10223)1.0µL splitless injection of sympathomimetic aminesConc.: approximately 2.5ng/µL.


Inj. temp.: 225°CInterface temp.: 275°CDet.: MSDIonization: EICarrier gas: heliumLinear velocity: 20cm/sec. set @ 100°CSplitless hold time: 1 min.

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705Solvents


10 11

85

min. 5 10 15 20 25 30 35 40 45 50 55 60

1

2

3

4

6,79

1217

14

15

16

18

19

20

21

22

23 24

25

26

27

28

29

30

31

32

33 34

35

36

37

38 39

Solvent Mixture #1Rtx®-1

13

GC_CH00335

60m, 0.53mm ID, 3.0µm Rtx®-1 (cat.# 10188)1.0µL split injection of solvent mix #1

Oven temp.: 40°C (hold 5 min.) to 285°C @ 5°C/min.Inj. / det. temp.: 275°C / 285°CMSD: full scanCarrier gas: heliumLinear velocity: 40cm/sec.Split vent: 50mL/min.

31

6


min. 5 10 15 20 25 30 35 40 45 50 55 60

2

1

4

3

5

7

11 10

8

9

12

14

23

19

13

22

15

17

27

18

2429

25

3837

36

33

35

26

28

32

34

39

GC_CH00336

60m, 0.53mm ID, 3.0µm Rtx®-200 (cat.# 15088)1.0µL split injection of solvent mix #1.

Oven temp.: 40°C (hold 5 min.) to 285°C @ 5°C/min.Inj. / det. temp.: 275°C / 285°CMSD: TIC modeCarrier gas: heliumLinear velocity: 40cm/sec.Split vent: 50mL/min.

Refer to peak list on page 709.


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S706 Solvents


Solvent Mixture #1Stabilwax®

min. 10 20 30 40 50 60 70

1

4

2

11

1023

12

27

6,13

1529

8

5

19

34

28

3

24

9

32

3531

7

36

37

17

18

38

33

26

25

39

60m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10658)1.0µL split injection of solvent mix #1

Oven temp.: 40°C (hold 5 min.) to 250°C @ 5°C/min.Inj. / det. temp.: 275°C / 285°CMS: full scanCarrier gas: heliumLinear velocity: 40cm/sec.Split vent: 50mL/min.

22,14

GC_CH00337


min. 5 10 15 20 25 30 35 40 45 50 55

1

2

40

41

42

4

4344

47

48

49

50

45,46

11

51

52

5354

55

56

57

58 23

5960

61

29

6364

65

34

62

66

6768

35

69



GC_CH00338



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707Solvents





69

min. 5 10 15 20 25 30 35 40 45 50 55

1

2

4

40

42

46

45

11

49

41

50

47 52

23

44

53 54

56

48

57

2958

61

59

62,34

636466

35

65

67 68

GC_CH00339


min. 5 10 15 20 25 30 35 40 45 50 55 60

1

2

4

11

46

23

4249

53

4540

29

54

4752

57

44

58

48

3459

50

35

63

61

41

56

62

64

55

68

66

67

65



GC_CH00340



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S708 Solvents


81


1

min. 5 10 15 20 25 30 35 40 45 50

2

7071

72

73

4

74,75

76,7778

7980 82

8384

85,86

87

89

88,11

90

91

92

93

94

23

95

96

97

98

99

100

101 29

34

102

35



GC_CH00341


1

min. 5 10 15 20 25 30 35 40 45

2

70

4

73

77 72 78

76

75

80,11

82

74

8381 79

86

92,87

89

84

90

95,11

85,91

88

29

98

93

99

100

34

102

35

GC_CH00342





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709Solvents




754


min. 5 10 15 20 25 30 35 40 45 50

2

1

11

72

78,73

76

81

23

70

74

95

89

79

92

80

8429

90

8284

86

87

91,34

93

98

85

99

88

100

83

102

GC_CH00343

1. pentane2. methylene chloride3. ethylene glycol4. heptane5. cyclopentanol6. 3-hexanol7. acetamide8. 2-methyl-1-pentanol9. furfuryl alcohol

10. butyl ether11. nonane12. cumene13. ethyl amyl ketone14. heptanol15. butyl butanoate16. ——17. benzyl alcohol18. dipropylene glycol19. diethylbenzene20. ——21. ——22. hexachloroethane23. undecane24. 1-nonanol25. p-methoxyphenol26. triethylene glycol

27. dodecane28. undecanal29. tridecane30. ——31. dodecanal32. dicyclohexylamine33. bis(2,2-methoxy)ethyl ether34. pentadecane35. heptadecane36. octadecane37. nonadecane38. eicosane39. acetyl tributyl citrate40. 2-buten-1-ol41. formamide42. 3-pentanol43. 1-nitropropane44. dimethylformamide45. 2-methyl-3-pentanol46. toluene47. ethyl chloroacetate48. dimethylacetamide49. p-xylene50. sec-tetrachloroethane51. benzaldehyde52. o-chlorotoluene

53. 2,6-dimethyl-4-heptanone54. 2-octanone55. o-cresol56. α-methylbenzyl alcohol57. 5-nonanone58. nonanal59. decanal60. ——61. 1-decanol62. 1-undecanol63. 2-dodecanone64. 1-dodecanol65. tetraethylene glycol66. dibenzyl67. diethyl phthalate68. tributyl phosphate69. diphenyl sulfone70. allyl alcohol71. ——72. isopropyl acetate73. benzene74. 2-nitropropane75. nitroethane76. pentanal77. 2-bromobutane78. 1-chloropentane

79. cyclopentanone80. 2-hexanol81. butyl acetate82. 2-ethyl-1-butanol83. 3-ethyl-3-pentanol84. 1,4-dichlorobutane85. 2-methyl-2,4-pentanediol86. butoxyethanol87. 1,2,3-trichloropropane88. 1,4-butanediol89. methyl hexanoate90. 1,2,4-trimethylbenzene91. 2-ethyl-1-hexanol92. dipentene/limonene93. tetrahydrofurfuryl acetate94. ——95. decahydronaphthalene96. ——97. ——98. 2-decanol99. 1,2-bis(2-methoxyethoxy)ethane

100. 2-phenoxyethanol101. —-102. benzyl ether

Components of solvent mixture #1, #2, #3 (see pages 705–709 for chromatography)

applicationsTable of Contentssee ppaaggeess 446600--446633

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SolventsRtx®-200

GC_CH00349

1

2

3

4

5

67

8

9

10

11

12,13

15

14

16

17

18

19

1. methanol2. trichlorotrifluoroethane3. methylene chloride4. chloroform5. cyclohexane6. carbon tetrachloride7. acetone8. isobutyl alcohol9. acetonitrile

10. benzene11. tetrahydrofuran12. trichloroethylene13. ethylene dichloride14. ethyl acetate15. methyl ethyl ketone16. toluene17. pyridine18. chlorobenzene19. methylisoamyl ketone

85m, 0.53mm ID, 3.0µm Rtx®-200 (cat.# 50762)0.1µL direct injection of a solvent mixture


Inj. / det. temp.: 250°C / 270°CCarrier gas: heliumLinear velocity: 31cm/sec.TCD sensitivity: range = 0.50, atten. = 4

min. 4 8 12 16 20 24 28 32

Solvents Rtx®-1701

1. water2. 2-butanone3. 2-butanol

1

2

3

min. 4 8

GC_CH00350


Oven temp.: 55°CInj. & det. temp.: 225°CCarrier gas: heliumLinear velocity: 20cm/sec.TCD sensitivity: 8 mVSplit ratio: 40:1

Solvents Rtx®-1701

1. acetaldehyde2. methanol3. acetone4. methyl acetate5. vinyl acetate6. cyclohexane7. ethyl acetate8. crotonaldehyde

1

2

3

45

7

6

8

min. 2 4 6 8

60m, 0.53mm ID, 1.5µm Rtx®-1701 (cat.# 12073)0.4µL direct injection of a solvent mixtureConc.: 2000 ppm

Oven temp.: 40°C (hold 4 min.) to 100°C @15°C/min. (hold until last compound elutes)


GC_CH00351

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SolventsRtx®-1

1

2

3

4

5

67

8

12

13

14,15

16,17

18

19

20

min. 2 4 6 8 10 12 14 16 18

9,10,11

30m, 0.53mm ID, 5.0µm Rtx®-1 (cat.# 10179)0.5mg/mL wet needle injection of a solvent mixDirect injection using a Uniliner® liner

Oven temp.: 40°C (hold 4 min.) to 110°C @ 4°C/min. to 175°C @15°C/min. (hold 5 min.)


GC_CH00344

1. methanol2. methyl formate3. acetone4. pentane5. methylene chloride6. n-propyl alcohol7. methyl ethyl ketone8. sec-butyl alcohol9. ethyl acetate

10. chloroform11. hexane12. tetrahydrofuran13. 1,1,1-trichloroethane14. isobutyl alcohol15. benzene16. n-butyl alcohol17. ethoxyethane18. toluene19. n-butyl acetate20. o-xylene

1. pentane2. hexane3. methyl formate4. acetone5. tetrahydrofuran6. 1,1,1-trichloroethane7. ethyl acetate8. methyl ethyl ketone9. methanol

10. methylene chloride11. chloroform12. benzene13. n-propyl alcohol14. toluene15. sec-butyl alcohol16. n-butyl acetate17. isobutyl alcohol18. n-butyl alcohol19. o-xylene20. ethoxyethane

SolventsStabilwax®

min. 2 4 6 8 10

1

2

3

4

5

67

8,9

10

11,12

13

14

15

16

1718

19

20

GC_CH00345

30m, 0.25mm ID, 0.25µm Stabilwax® (cat.# 10623)0.5µL split injection of a solvent mixture

Oven temp.: 40°C (hold 4 min.) to 250°C @ 5°C/min.Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 3.2cc/min.)FID sensitivity: 16 x 10-11 AFSSplit ratio: 40:1

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SolventsRtx®-5

1. acetone2. acrylonitrile3. carbon disulfide impurity4. hexane5. methyl ethyl ketone6. ethyl acetate7. methyl Cellosolve®

8. tetrahydrofuran9. n-butyl alcohol

10. isopropyl acetate11. heptane12. n-propyl acetate13. amyl alcohol14. toluene15. n-butyl acetate16. n-butyl acrylate17. Cellosolve® acetate18. cyclohexanone

min. 4 8 12 16 20 24

1

2

3

4

5

6

9,10

7,8

11

12

13

14 15

1617

18

GC_CH00346

60m, 0.32mm ID, 3.0µm Rtx®-5 (cat.# 10287)1.0µL split injection of solvents collected on a charcoal tube andextracted in carbon disulfide


Inj. & det. temp.: 300°CCarrier gas: hydrogenLinear velocity: 40cm/sec. set @ 50°CFID sensitivity: 128 x 10-12 AFS

Permission to publish this chromatogram granted by Kenda McCoy,Rexham Industrial.

Volatile SolventsRtx®-Wax

1. acetaldehyde2. acetone3. ethyl formate4. ethyl acetate5. methanol6. ethanol7. n-propanol8. isobutanol

30m, 0.53mm ID, 1.0µm Rtx®-Wax (cat.# 12455)0.1µL split injection of volatile solvents

Oven temp.: 20°C (hold 10 min.) to 100°C @ 5°C/min.Inj. & det. temp.: 200°CCarrier gas: heliumLinear velocity: 40cm/sec. set @ 20°CFID sensitivity: 8 x 10-11 AFSSplit ratio: 10:1

GC_CH00347

min. 5 10 15 20

1

2

34

5 6

7

8Searching for a chromatogram?

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713Solvents


Solvents Rtx®-Volatiles

60m, 0.53mm ID, 2.0µm Rtx®-Volatiles (cat.# 10905)1.0µL split injection of neat solvents


Inj. & det. temp.: 250°CCarrier gas: heliumLinear velocity: 20cm/sec. (flow rate: 2.6cc/min.)TCD sensitivity: 4 x 10-11 AFSTCD current: 100mASplit ratio: 40:1

1

2

3

4

6

57

min. 4 8 12 16

GC_CH00352

1. water2. methanol3. ethanol4. isopropanol5. ethyl acetate6. isopropyl acetate7. 4-methyl-2-pentanone

Solvents, Industrial Stabilwax®-DA

30m, 0.53mm ID, 1.0µm Stabilwax®-DA (cat.# 11055)0.1µL direct injection of industrial solvents

Oven temp.: 95°C (hold 4 min.) to 250°C @ 4°C/min.Inj. & det. temp.: 250°CCarrier gas: heliumLinear velocity: 40cm/sec. (flow rate: 5cc/min.)FID sensitivity: 32 x 10-11 AFS

2

4

1 3

5

GC_CH00353

1. 1,4-dioxane2. styrene3. α-methylstyrene4. dipropylene glycol

monomethylether5. acrylic acid

min. 4 8 12 16 20 24

Solvents, Industrial MXT®-WAX

30m, 0.53mm ID, 1.0µm MXT®-WAX (cat.# 70655)Wet needle injection of neat solvents

Oven temp.: 50°C to 220°C @ 10°C/min.Inj. & det. temp.: 250°CCarrier gas: heliumLinear velocity: 20cm/sec.FID sensitivity: 5.12 x 10-10 AFS

1

2

3

4

5

8,9

6,710,11

15

16

12

13

14

19

20

GC_CH00354

17 18

min. 2 4 6 8 10

Solvents, Industrial Stabilwax®

15

14

GC_CH00355

1. pentane2. hexane3. methyl formate4. acetone5. tetrahydrofuran6. 1,1,1-trichloroethane7. ethyl acetate8. methanol9. methyl ethyl ketone

10. methylene chloride11. chloroform12. benzene13. n-propyl alcohol14. sec-butyl alcohol15. toluene16. n-butyl acetate17. isobutyl alcohol18. n-butyl alcohol19. o-xylene20. ethoxyethane

30m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10655)Wet needle injection of neat solvents

Oven temp.: 50°C to 220°C @ 10°C/min.Inj. & det. temp.: 250°CCarrier gas: heliumLinear velocity: 20cm/sec.FID sensitivity: 5.12 x 10-10 AFS

3

4

5

8,9

6,72

1

10,11

16

13 1718

19

20

12

min. 2 4 6 8 10

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714


SolventsRt-Q PLOT™

1. methane2. acetaldehyde3. acetonitrile4. acetone5. methylene chloride6. n-pentane7. ethyl acetate8. n-hexane9. benzene

30m, 0.53mm ID Rt-Q PLOT™ (cat.# 19716)70µL split injection of solvent mixture

Oven temp.: 100°C to 220°C @ 15°C/min. (hold 2 min.)Inj. & det. temp.: 220°CCarrier gas: heliumLinear velocity: 23.6cm/sec. set @ 100°CFID sensitivity: 1.28 x 10-10 AFSSplit ratio: 7.7:1Split: 44.5cc/min.Column flow: 6.6cc/min.

min. 2 4 6 8 10

GC_CH00348

1

23

4

5

6

7

8

9

Solvents, Polar Rt-QPLOT™

30m, 0.32mm ID Rt-QPLOT™ PLOT (cat.# 19718) 20µL split injection. 50ppm (w/v) each in helium with a Cyclosplitter® liner (cat.# 20755)

Oven temp.: 150°CInj./det. temp.: 200°CCarrier gas: hydrogenDet.: FIDSplit ratio: 20/1

1. methane2. methanol3. ethanol4. acetone5. n-pentane6. ethyl acetate7. n-hexane

4

2

35

6

7

1

min. 1 2 3 4 5 6 7 8 9 10 11 12GC_PC00181

30m, 0.32mm ID Rt-UPLOT™ PLOT (cat.# 19724)20µL split injection. 50ppm (w/v) each in helium with a Cyclosplitter® liner (cat.#20755)

Oven temp.: 150°CInj./det. temp.: 200°C Carrier gas: hydrogenDetector: FIDSplit ratio: 20/1

1. methane2. methanol3. ethanol4. n-pentane 5. acetone6. n-hexane7. ethyl acetate

4

2

3

5

6

7

1

Solvents, Polar Rt-UPLOT™

min. 1 2 3 4 5 6 7 8 9 10 11 12GC_PC00182

SolventsHayeSep® Q

5

7

8

6

4

3

2

1

min. 4 8

1. methanol2. ethanol3. acetonitrile4. acetone5. methylene chloride6. n-pentane7. chloroform8. n-hexane

2m, 1mm ID HayeSep® Q (cat.# 19017)1µL direct injection of a neat solvent mixture


Inj. & det. temp.: 200°CCarrier gas: heliumFlow: 20mL/min. set @ 40°CFID sensitivity: 512 x 10-11 AFS

GC_CH00328

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715Solvents


Solvents, USPRtx®-1

1. formaldehyde2. water3. chloromethane4. methanol5. acetaldehyde6. ethylene oxide7. chloroethane8. ethanol9. acetonitrile

10. acetone11. 2-propanol12. 2-chloropropane13. diethyl ether14. pentane15. ethyl formate16. formic acid17. methylal18. 1,1-dichloroethene19. methyl acetate20. methylene chloride

21. nitromethane22. 1-propanol23. trans-1,2-dichloroethene24. methyl tert-butyl ether25. 2-methylpentane (spiked at 9%)26. 2-butanone (MEK)27. 2-butanol28. cis-1,2-dichloroethene29. acetic acid30. isopropyl ether31. ethyl acetate32. hexane33. chloroform34. tetrahydrofuran35. 2-methoxyethanol36. 1,2-dichloroethane37. methyl cyclopentane38. 1,1,1-trichloroethane39. 1,2-dimethoxyethane40. methyl isopropyl ketone

41. 2,2-dimethoxypropane42. isopropyl acetate43. 1-butanol44. benzene45. carbon tetrachloride46. ethylene glycol47. formamide48. 1,4-dioxane49. trichloroethene50. isooctane51. 2-ethoxyethanol52. n-heptane (C7)53. isoamyl alcohol54. hexanone (MIBK)55. pyridine56. methyl cyclohexane57. dimethyl formamide (DMF)58. 1,1,2-trichloroethane59. 1-pentanol 60. isobutyl acetate

61. toluene62. 2-hexanone (MBK)63. dimethyl sulfoxide64. butyl acetate65. 1,1-diethoxypropane66. N,N-dimethylacetamide67. chlorobenzene68. ethylbenzene69. isoamyl acetate70. p-xylene71. m-xylene72. o-xylene73. anisole74. isopropylbenzene (cumene)75. 1-methyl-2-pyrrolidinone76. sulfolane77. 1,2,3,4-tetrahydronaphthalene

*paraldehyde

Rtx®-1 60m, 0.53mm, 3.00µm (cat.# 10188)Sample: solvents Conc.: ~1.3% eachSample size: wet needleInj.: split, 250°CSeptum purge: 5mL/min.Split vent flow: 100mL/min. ~1:13 splitCarrier: heliumHead pressure: 11.0psi constant pressureLinear velocity: 45.6 cm/sec @ 35°CDet: mass selectiveScan range: 10amu to 260amuMS interface: open split interface ~1:7 splitOven temp.: 35°C (hold 4 min.) to 250°C @ 4°/min.

GC_EV00463

for more infoSeparation on an Rtx®-200 column on ppaaggee 771166. Also seeppaaggeess 669999––770011.

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S716 Solvents


Solvents, USPRtx®-200

1. formaldehyde2. water3. chloromethane4. methanol5. acetaldehyde6. ethylene oxide7. chloroethane8. ethanol9. acetonitrile

10. acetone11. 2-propanol12. 2-chloropropane13. diethyl ether14. pentane15. ethyl formate16. formic acid17. methylal18. 1,1-dichloroethene19. methyl acetate20. methylene chloride

21. nitromethane22. 1-propanol23. trans-1,2-dichloroethene24. methyl tert-butyl ether25. 2-methylpentane (spiked at 9%)26. 2-butanone (MEK)27. 2-butanol28. cis-1,2-dichloroethene29. acetic acid30. isopropyl ether31. ethyl acetate32. hexane33. chloroform34. tetrahydrofuran35. 2-methoxyethanol36. 1,2-dichloroethane37. methyl cyclopentane38. 1,1,1-trichloroethane39. 1,2-dimethoxyethane40. methyl isopropyl ketone

41. 2,2-dimethoxypropane42. isopropyl acetate43. 1-butanol44. benzene45. carbon tetrachloride46. ethylene glycol47. formamide48. 1,4-dioxane49. trichloroethene50. isooctane51. 2-ethoxyethanol52. n-heptane (C7)53. isoamyl alcohol54. hexanone (MIBK)55. pyridine56. methyl cyclohexane57. dimethyl formamide (DMF)58. 1,1,2-trichloroethane59. 1-pentanol 60. isobutyl acetate

61. toluene62. 2-hexanone (MBK)63. dimethyl sulfoxide64. butyl acetate65. 1,1-diethoxypropane66. N,N-dimethylacetamide67. chlorobenzene68. ethylbenzene69. isoamyl acetate70. p-xylene71. m-xylene72. o-xylene73. anisole74. isopropylbenzene (cumene)75. 1-methyl-2-pyrrolidinone76. sulfolane77. 1,2,3,4-tetrahydronaphthalene

Rtx®-200 60m, 0.53mm, 3.00µm (cat.# 15088)Sample: solvents Conc.: solventless mixture ~1.3% eachSample size: wet needleInj.: split, 250°CSeptum purge: 5mL/min.Split vent flow: 100mL/min. ~1:13 splitCarrier: heliumHead pressure: 11.0psi constant pressureLinear velocity: 45.6 cm/sec @ 35°CDet: mass selectiveScan range: 10amu to 260amuMS interface: open split interface ~1:7 splitOven temp.: 35°C (hold 4 min.) to 250°C @ 4°/min.

GC_EV00464

for more infoSeparation on an Rtx®-1 column on ppaaggee 771155. Also seeppaaggeess 669999––770011.

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717Solvents


Pentane (lot purity analysis)MXT®-1

1

min. 4 8

1. pentane

LOT B

60m, 0.53mm ID, 5.0µm MXT®-1 (cat.# 70183)1.0µL direct injection of high purity pentane

Oven temp.: 40°CInj. & det. temp.: 200°CCarrier gas: heliumLinear velocity: 40cm/sec. (flow rate: 10cc/min.)FID sensitivity: 4 x 10-11 AFS

GC_CH00332

1

min. 4 8

LOT A

Solvents, Nitrogen Containing Rtx®-1701

1

2

3

4

5

6

8

7

1. butyronitrile2. toluene3. pyrazine4. pyridine5. pyrimidine6. 2-picoline7. 3-picoline8. 4-picoline

GC_CH00330

min. 2 4 6 8 10

30m, 0.32mm ID, 1.0µm Rtx®-1701 (cat.# 12054)1.0µL split injection of a neat pyridine standard

Oven temp.: 80°C (hold 4 min.) to 130°C @ 5°C/min.Inj. / det. temp.: 200°C / 260°CCarrier gas: heliumLinear velocity: 26cm/sec.FID sensitivity: 1.28 x 10-10 AFSSplit vent: 40cc/min.


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S718 Solvents


Substituted PyridineRtx®-1701

21 3 4 5 6 7

8

9 10 11

min. 4 8 12 16 20 24 28 32GC_CH00333

60m, 0.53mm ID, 3.0µm Rtx®-1701 (cat.# 12088)0.1µL direct injection of a substituted pyridine standard (0.5-5% per component), Uniliner® liner

Oven temp.: 60°C to 225°C @ 5°C/min.Inj. & det. temp.: 230°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.1cc/min.)FID sensitivity: 32 x 10-11 AFS

1. methanol2. benzene3. toluene4. pyridine5. α-picoline6. 2,6-lutidine7. β-picoline8. 4-cyanopyridine9. 3-cyanopyridine

10. 2-cyanopyridine11. picamide

Substituted PyridineStabilwax®

min. 4 8 12 16 20 24 28 32 36

1 2 43 5 76

8

9 10 11

GC_CH00334

30m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10655)0.1µL direct injection of a substituted pyridine standard (0.5-5% per component), Uniliner® liner

Oven temp.: 60°C to 225°C @ 5°C/min.Inj. & det. temp.: 230°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.1cc/min.)FID sensitivity: 32 x 10-11 AFS

1. methanol2. benzene3. toluene4. pyridine5. α-picoline6. 2,6-lutidine7. β-picoline8. 4-cyanopyridine9. 3-cyanopyridine

10. 2-cyanopyridine11. picamide

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719Alcohols


1. 2,2,2-trifluoroethanol2. pentane3. 2-methyl-1-propanol4. 3-buten-2-ol5. 2-methyl-3-buten-2-ol6. 2-methoxyethanol7. 1-penten-3-ol8. ethylene glycol9. 2-ethoxyethanol

10. 1-pentanol11. 2-methyl-1-pentanol

12. trans-2-hexen-1-ol13. 2,2-dimethyl-1-pentanol14. 1,4-butanediol15. undecane16. 1-octanol17. tetradecane18. 1-undecanol

AlcoholsRtx®-502.2

min. 5 10 15 20 25 30

2

1615 17 1816

1514

11

10

1312

8

9

7

6

54

32

1

13

14

11

12

10

8,97

6

54

31

1. pentane2. isopropyl alcohol3. 2-propen-1-ol4. 2-butanol5. 2-pentanol6. 1-pentanol7. 2-hexanol8. 2-heptanol9. 2-octanol

10. undecane11. 1-octanol12. 2-nonanol13. 2-decanol14. 2-phenoxyethanol15. tetradecane16. 1-undecanol

GC_CH00286

105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910)1.0µL split injection of an alcohol mixture


Inj. / det. temp.: 250°C / 270°CDet. : MSCarrier gas: heliumLinear velocity: 56cm/sec. set @ 50°CSplit ratio: 7:1Ionization: EI

min. 5 10 15 20 25 30

1. pentane2. 3-buten-1-ol3. 3-methyl-1-butanol4. 3-methyl-3-pentanol5. 1-pentanol6. 3-methyl-2-buten-1-ol7. 3-hexen-1-ol8. 4-heptanol9. tetrahydrofurfuryl alcohol

10. 2-(2-ethoxyethoxy)ethanol11. undecane12. 1-octanol13. α-methylbenzyl alcohol14. 2-(2-butoxyethoxy)ethanol15. tetradecane16. 1-undecanol

AlcoholsRtx®-502.2

105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910)1.0µL split injection of an alcohol mixture


Inj. / det. temp.: 250°C / 270°C Det. : MSCarrier gas: heliumLinear velocity: 56cm/sec. set @ 50°CSplit ratio: 7:1Ionization: EI

1

2

3

4

5

67

8

9

12

13

14

16

1011

15

min. 5 10 15 20 25 30GC_CH00290

min. 5 10 15 20 25 30

1

2

34

5

6

78

910

11

12

13

14

GC_CH00291

1. pentane2. 2-buten-1-ol3. 3-methyl -2-butanol4. 3-pentanol5. 4-methyl-2-pentanol6. 1-pentanol7. 3-hexanol8. cyclopentanol9. 2-furanmethanol

10. cyclohexanol11. hexylene glycol12. 3-octanol13. undecane14. 1-octanol15. tetradecane16. 1-undecanol

15

16

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S720 Alcohols


AlcoholsStabilwax®

1. pentane2. hexane3. heptane4. ethanol5. decane6. 1-propanol7. 1-butanol8. dodecane9. 1-pentanol

10. 1-hexanol11. tetradecane12. 1-heptanol13. 1-octanol14. hexadecane15. 1-nonanol16. 1-decanol17. 1-undecanol18. 1-dodecanol

GC_CH00288

60m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10658)1.0µL split injection of alcohols


Inj. & det. temp.: 250°CDet. : MSCarrier gas: heliumLinear velocity: 51cm/sec. set @ 50°CSplit ratio: 8:1Ionization: EI

1. pentane2. methanol3. 2-methyl-2-propanol (tert-butylalcohol)4. 2-methyl-3-buten-2-ol5. 3-buten-2-ol6. undecane7. 2,2-dimethyl-1-propanol (neopentanol)8. 1-penten-3-ol9. 2-methoxyethanol

10. 2,2,2-trifluoroethanol11. 2-ethoxyethanol12. 1-pentanol13. 2-methyl-1-pentanol14. 2,2-dimethyl-1-pentanol15. tetradecane16. trans-2-hexen-1-ol17. 1-octanol18. ethylene glycol19. 1-undecanol20. 1,4-butanediol

7

2

1

min. 5 10 15 20 25

18

17

16

15

13

1412

10

11

98

654

3

1

23

89

10

16

19

20

18

17

15

14

13

11

12

7

6

54

min. 5 10 15 20 25

min5 1010 1515 2020 2525 3030 35

1

2

3

4

5

6

7

8

9

1011

30m, 0.32mm ID, 10µm Rt-QPLOT™ (cat.# 19718)1.0µL split injection of alcohol mixtureConc.: 1% each in water

Oven program: 100°C to 240°C @ 5°C/min. (hold 10 min.)Injector: split @ 250°CCarrier gas: helium (constant pressure mode)Head pressure: 18.0psiColumn flow rate: 1.1cc/min. @ 100°CLinear velocity: 31cm/sec. @ 100°CSplit ratio: 70:1Detector: FID @ 270°CMake-up gas flow: 45cc/min.Inlet liner: 4mm single gooseneck (cat.# 20798)

1. methanol2. ethanol3. 2-propanol4. 1-propanol5. tert-butanol6. 2-butanol7. isobutyl alcohol8. 1-butanol9. 2-methyl-2-butanol

10. 3-methyl-1-butanol11. 4-methyl-2-pentanol

GC_CH00495

AlcoholsRt-QPLOT™

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721Alcohols; Alcohols & Aldehydes


AlcoholsRtx®-5

GC_CH00292

min. 2 4 6 8 10 12 14

1

2

34

5

6

7 89

10

11

60m, 0.32mm ID, 1.0µm Rtx®-5 (cat.# 10257)0.03µL split injection of a neat alcohols mix


Inj. & det. temp.: 200°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 3.2cc/min.)FID sensitivity: 128 x 10-11 AFSSplit ratio: 40:1

1. methanol2. ethanol3. acetone4. isopropanol5. 1-propanol6. ethyl acetate7. 2-methyl-1-propanol8. 1-butanol9. 3-pentanol

10. 3-methyl-1-butanol11. 2-methyl-1-butanol

AlcoholsRtx®-Volatiles

GC_CH00293min. 2 4 6 8 10 12 14

1110

98

7

6

5

4

3

2

1

60m, 0.32mm ID, 1.5µm Rtx®-Volatiles (cat.# 10904)0.03µL split injection of a neat alcohols mix


Inj. & det. temp.: 200°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 3.2cc/min.)FID sensitivity: 64 x 10-11 AFSSplit ratio: 40:1

1. methanol2. ethanol3. isopropanol4. acetone5. 1-propanol6. ethyl acetate

7. 2-methyl-1-propanol8. 1-butanol9. 3-pentanol

10. 3-methyl-1-butanol11. 2-methyl-1-butanol

Alcohols & AldehydesRtx®-502.2

GC_CH00294min. 15 20 25 30 35 40

14

13

12

11

10

9

876

5

4

3

2

1

1. heptane2. 1-penten-3-ol3. 3-pentanol4. ethylene glycol5. furfural6. 2-furanmethanol7. cyclohexanol8. 2-methyl-2,4-pentanediol9. 1,4-butanediol

10. 3-octanol11. diethylene glycol12. benzaldehyde13. pentadecane14. nonadecane

105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910)1.0µL split injection of alcohols and aldehydes


Inj. / det. temp.: 250°C / 285°CCarrier gas: heliumLinear velocity: 50cm/sec. set @ 50°CSplit ratio: 7:1Ionization: EIMode: scan 10, range 300

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S722 Alcohols & Aldehydes; Aldehydes


Alcohols & AldehydesStabilwax®

min. 5 10 15 20 25GC_CH00295

17

16

15

14

12,13

11

10

9

7

4

3

21

1. pentane2. heptane3. acetaldehyde4. dimethoxymethane5. butyraldehyde6. 3-pentanol7. 1-penten-3-ol8. 3-octanol9. cyclohexanol

10. furfural11. pentadecane12. benzaldehyde13. ethylene glycol &

2-methyl-2,4-pentanediol14. furfuryl alcohol15. nonadecane16. 1,4-butanediol17. diethylene glycol

60m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10658)1.0µL split injection of alcohols and aldehydes



5

6

8

AldehydesStabilwax®

60m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10658)1.0µL split injection of aldehydes

Oven temp.: 45°C (hold 10 min.) to 250°C @ 12°C/min. (hold 20 min.).Inj. & det. temp.: 250°CDet.: MSDCarrier gas: heliumLinear velocity: 51cm/sec. set @ 50°CSplit ratio: 8:1Ionization: EI

5

17

1

23

4

6

78

9,10

11

12

13

14

19

1816

15

min. 5 10 15 20 25

GC_CH00315

1. pentane2. acetaldehyde3. dimethoxymethane

(methylal)4. propanal5. 2-methyl propanal6. methanol7. pentanal8. 2-butenal

(crotonaldehyde)9. hexanal

10. undecane11. heptanal12. 1-pentanol13. octanal14. tetradecane15. nonanal16. 2-furancarboxaldehyde17. decanal18. benzaldehyde19. 1-octanol


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723Aldehydes; Acrylates


AldehydesRtx®-Wax

30m, 0.25mm ID, 0.50µm Rtx®-Wax (cat.# 12438)split injection of C2-C11 aldehydes mixtureOn-column conc.: 250ng

Oven temp.: 40°C (hold 5 min.) to 200°C @10°C/min.Inj. & det. temp.: 200°CCarrier gas: hydrogenLinear velocity: 35cm/sec. set @ 40°CFID sensitivity: 82 x 10-11 AFSSplit ratio: 100:1

1. ethanal2. propanal3. butenal4. pentanal5. hexanal6. heptanal7. octanal8. nonanal9. decanal

10. undecanal

GC_CH00300min. 5 10 15 20

1

2

3 45

6 7

8

9

10

4

FormaldehydeRtx®-1701

min. 2 4 6 8

1 2

3

5

60m, 0.25mm ID, 1.0µm Rtx®-1701 (cat.# 12056)0.5µL split injection of a formaldehyde sample

Oven temp.: 40°CInj. / det. temp.: 150°C / 175°CCarrier gas: heliumLinear velocity: 20cm/sec. (flow rate: 0.8cc/min.)TCD sensitivity: 8 mVSplit vent: 30cc/min.

1. air2. water3. formaldehyde4. acetaldehyde5. methanol

GC_CH003134

AcrylatesRtx®-1701

min. 4 8 12 16 20 24

12 6

5

7 9 11

108

1. n-butanol2. methyl methacrylate3. n-butyl acetate4. isopropyl methacrylate5. n-butyl ether6. isobutyl isobutyrate7. isobutyl methacrylate8. n-butyl isobutyrate9. butyl methacrylate

10. n-butyl n-butyrate11. n-butyl α-hydroxyisobutyrate

30m, 0.32mm ID, 1.0µm Rtx®-1701 (cat.# 12054)0.5µL split injection of acrylates


Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec.FID sensitivity: 4 x 10-11 AFSSplit ratio: 56:1

3

GC_CH00312

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S724 Amines


EthanolaminesRtx®-5 Amine

1

2

3

4

min. 5 10

1. monoethanolamine2. diethanolamine3. triethylene

glycol monomethylether (IS)4. triethanolamine

15m, 0.25mm ID, 0.50µm Rtx®-5 Amine (cat.# 12335)1.0µL split injection of ethanolamine mix in methanolOn-column conc.: 34ng


Inj. & det. temp.: 280°C / 300°CCarrier gas: hydrogenLinear velocity: 43cm/sec. set @ 50°CFID sensitivity: 6.4 x 10-11 AFSSplit ratio: 58:1

GC_CH00296Rtx®-35 Amine 30m, 0.32mm ID, 1.0µm (cat.# 11354)Sample: 500µg/mL ethanolamine standard in water Inj.: 1.0µL split (split ratio 10:1),

cup splitter inlet liner (cat.# 20709)Inj. temp.: 300°CCarrier gas: helium, constant pressure Linear velocity: 40cm/sec. @ 50°COven temp.: 50°C (hold 0.50min.) to 280°C @15°C/min. Det.: FID @300°C

1. monoethanolamine2. diethanolamine3. triethyleneglycol monomethylether4. triethanolamine

GC_CH00585

EthanolaminesRtx®-35 Amine

EthyleneaminesRtx®-5 Amine

1 23

4

5

6

7

min. 5 10 15 20 25GC_CH00298

30m, 0.25mm ID, 0.50µm Rtx®-5 Amine (cat.# 12338)3.0µL split injection of ethyleneamine industrial sample On-column conc.: ~5-80ng


Inj. & det. temp.: 315°CCarrier gas: hydrogenLinear velocity: 43cm/sec. set @ 40°CFID sensitivity: 6.4 x 10-11 AFSSplit ratio: 20:1

1. isopropanol2. monoethanolamine3. ethylenediamine4. piperazine5. diethylenetriamine6. aminoethylethanolamine7. aminoethylpiperazine

remaining impurities consist of ethyleneamine and piperazine deriv-atives


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725Amines


Amines & PhenolsRtx®-5 Amine

1. diethylamine2. pyridine3. morpholine4. phenol5. aniline6. 2-chlorophenol7. diethylenetriamine8. octylamine9. 1-methyl-2-pyrrolidinone

10. 2-nitrophenol11. 2,6-dimethylaniline12. nicotine13. 2-nitroaniline

30m, 0.32mm ID, 1.0µm Rtx®-5 Amine (cat.# 12354)1.0µL split injection of miscellaneous amines and phenols in waterOn-column conc.: 22ng

Oven temp.: 120°C to 220°C @ 10°C/min.Inj. & det. temp.: 305°CCarrier gas: hydrogenLinear velocity: 38cm/sec. set @ 120°CFID sensitivity: 6.4 x 10-11 AFSSplit ratio: 25:1

GC_CH00301min. 2 4 6 8 10

1 2

3

4

5

6

7

8

9

10

11

12

13

1. cyclohexane2. hexamethyleneimine3. 1,4-diaminobutane4. pentamethylenediamine5. 1,2-diaminocyclohexane6. 1,5-diamino-2-methylpentane7. aminomethylcyclopentylamine8. hexamethylenediamine9. 6-aminocapronitrile

10. n-valeramide11. adiponitrile12. bis-hexamethylenetriamine

HexamethylenediamineStabilwax®-DB

30m, 0.32mm ID, 0.25µm Stabilwax-DB (cat.# 10824)0.4µL direct injection of a neat hexamethylenediamine sampleOn-column conc.: 10 to 1000ng/component


Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. FID sensitivity: 2 x 10-11 AFS

GC_CH00302

min. 4 8 12 16 20 24 28 32

1

2

3

4 5

6

7

8

9

10

11

12

30m, 0.53mm ID, 0.5µm Stabilwax®-DB (cat.# 10840)0.2µL direct injection of a neat hexamethylenediamine sample ontoa Uniliner® liner



min. 4 8 12 16 20 24 28

1

23

45

6

7

8

9

10

11

12

GC_CH00303

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S726 Amines


1. methylamine2. dimethylamine 3. trimethylamine4. ethylamine5. isopropylamine6. tert-butylamine7. n-propylamine8. diethylamine9. sec-butylamine

Primary AminesRtx®-35 Amine

1

2/3

4

5

6

78

9

GC_CH00578

Rtx®-35 Amine 30m, 0.53mm ID, 1.0µm (cat.# 11355)Sample: primary amines, 50ppm on-column conc. in waterInj.: 1.0µL, split (10:1), 4mm base-deactivated single

gooseneck (cat.# 20798-210.1)Injector: 250°CCarrier gas: helium, constant pressureLinear velocity: 35.7cm/secOven temp.: 35°C (hold 5.0 min.)Det.: FID @ 300°C

a plus 1 storyScott has used his training in computers to improve our QA processes for fusedsilica columns by building databases for RA testing tracking pass rate. Scottwrote the test methods and recorded all of the temperature profile data forapproximately one thousand tests.

SSccootttt SShhaarrpp,, Quality Assurance Analyst

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727Amines


Amines (low MW)Stabilwax®-DB

30m, 0.53mm ID, 1.0µm Stabilwax®-DB (cat.# 10855)1.0µL direct injection of amines in waterOven temp.: 45°CInj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5cc/min.)FID sensitivity: 1 x 10-11 AFSRecommended liner: Uniliner®

1. trimethylamine2. dimethylamine3. ethylamine4. methylamine5. isopropylamine6. n-propylamine7. tert-butylamine8. diethylamine9. sec-butylamine

1

2

3

4

5

6

7

89

GC_CH00304 min. 1 2 3

Amines (low MW)Stabilwax®-DB

1. trimethylamine2. triethylamine3. methanol

1

2

3

min. 4 GC_CH00305

30m, 0.53mm ID, 1.0µm Stabilwax®-DB (cat.# 10855)0.6µL split injection of low molecular weight amines in a methanol Conc.: 500ng/µLOven temp.: 50°CInj. & det. temp.: 200°CCarrier gas: hydrogenLinear velocity: 47cm/sec. (flow rate: 3.2cc/min.)FID sensitivity: 4 x 10-11 AFSSplit ratio: 40:1

Amines/Alcohols/Chlorides Stabilwax®

1

2

3

4

5

6 7

8

9 10

11

12

13 14

1617

15

min. 4 8 12 16 20GC_CH00307

1. n-octyl,N,N-dimethylamine2. n-octyl chloride3. n-decyl,N,N-dimethylamine4. n-decyl chloride5. n-octyl alcohol6. n-dodecyl,N,N-dimethylamine7. n-dodecyl chloride8. n-decyl alcohol9. n-tetradecyl,N,N-dimethylamine

10. n-tetradecyl chloride11. n-dodecyl alcohol12. n-hexadecyl,N,N-dimethylamine13. n-hexadecyl chloride14. n-tetradecyl alcohol15. n-octadecyl,N,N-dimethylamine16. n-hexadecyl alcohol17. n-octadecyl alcohol

30m, 0.53mm ID, .25µm Stabilwax® (cat.# 10625)0.5µL split injection


Inj. & det. temp.: 250°C Carrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.2cc/min.)FID sensitivity: 128 x 10-11 AFSSplit ratio: 40:1

NitrosaminesStabilwax®-DB

60m, 0.53mm ID, 1.0µm Stabilwax®-DB (cat.# 10858)Direct injection of nitrosaminesConc.: 1.0µg/mL

Oven temp.: 100°C (hold 1 min.) to 170°C @ 5°C/min. Inj. & det. temp.: 200°C Carrier gas: heliumLinear velocity: 100cm/sec. (flow rate: 15cc/min.)Detector: TSD

1. solvent2. N-nitrosodimethylamine3. N-nitrosodiethylamine4. N-nitrosodiisopropylamine5. N-nitrosodipropylamine6. N-nitrosodibutylamine7. N-nitrosopiperidine8. N-nitrosopyrrolidine9. N-nitrosomorpholine

987

6

5

43

2

1

min. 2 4 6 8 10 12 14GC_CH00306

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S728 Aromatics


AromaticsRtx®-502.2

1

2

3

4

5 6

7

8

9

12

13

10, 11

14

GC_CH00308

min. 35 40 45 50 55 60 65 70 75

1. o-xylene2. 1,3,5-trimethylbenzene3. α-methylstyrene4. 1,2,4-trimethylbenzene5. cymene6. 1,2,3-trimethylbenzene7. trans-decahydronaphthalene8. o-cresol9. cis-decahydronaphthalene

10. nitrobenzene11. diisopropylbenzene12. triethylbenzene13. pentadecane14. nonadecane

105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910)1.0µL split injection of an aromatics mixture


Inj. / det. temp.: 250°C / 280°CDet.: MSCarrier gas: heliumLinear velocity: 50cm/sec. set @ 50°CSplit ratio: 7:1Ionization: EI

60m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10658)1.0µL split injection of an aromatics mixture.


Inj. / det. temp.: 250°C / 280°CDet.: MSCarrier gas: heliumLinear velocity: 50cm/sec. set @ 50°CSplit ratio: 7:1Ionization: EI

1. trans-decahydronaphthalene2. o-xylene3. cis-decahydronaphthalene4. 1,3,5-trimethylbenzene5. p-cymene or p-isopropyltoluene6. 1,2,4-trimethylbenzene7. α-methyl benzene8. 1,2,3-trimethylbenzene9. diisopropylbenzene

10. triethylbenzene11. pentadecane12. nitrobenzene13. nonadecane14. o-cresol15. bibenzyl

GC_CH00309

min. 15 20 30 35 40 45 50 55

1

2

3

4

5

6

78

9

10

11

13

1514

12

AromaticsStabilwax®

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729Aromatics


AromaticsStabilwax®

60m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10658)1.0µL split injection of aromatics

Oven temp.: 45°C (hold 10 min.) to 250°C@ 12°C/min. (hold 20 min.)


1

2 3

4

5

6

8

7

9

10

11

12

min. 12 14 16 18 20 22 24 26 28GC_CH00311

1. toluene2. ethylbenzene3. p-xylene4. isopropyl benzene or cumene5. propylbenzene6. tert-butylbenzene

7. styrene8. butylbenzene9. pentadecane

10. naphthalene11. nonadecane12. p-cresol

AromaticsRtx®-502.2

4

5

6

7

8

9

10

11

12

13

min. 20 25 30 35 40

1

2

3

GC_CH00310

1. toluene2. ethylbenzene3. p-xylene4. styrene5. isopropyl benzene or cumene6. propylbenzene7. tert-butylbenzene8. butylbenzene9. p-cresol

10. naphthalene11. pentadecane12. bibenzyl13. nonadecane

105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910)1.0µL split injection of aromaticsOven temp.: 40°C (hold 10 min.) to 270°C @

12°C/min. (hold 20 min.)Inj. / det. temp.: 250°C / 285°CCarrier gas: heliumLinear velocity: 50cm/sec. set @ 50°CSplit ratio: 7:1Ionization: EIMode: scan 10, range 300

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S730 Chlorofluorocarbons


ChlorofluorocarbonsRtx®-1

3

2

1

4

5

6

7

8

9

min. 12 16 20 24 28 32

1.Freon® 222. Freon® 123. Freon® 1144. Freon® 1112a5. Freon® 1236. Freon® 123a7. Freon® 118. Freon® 1139. Freon® 122

GC_CH00319

105m, 0.25mm ID, 1.0µm Rtx®-1 (cat.# 10159)1.0µL split injection of a 99.9% pure Freon® 113 standard.


Inj. & det. temp.: 250°CDet.: full scanCarrier gas: heliumLinear velocity: 20cm/sec. set @ 35°CSplit vent: 30mL/min.


1

2

3

4

5

6 7 8 9

10

11 12

13

min. 10 15 20 25 30 35GC_CH00320

105m, 0.25mm ID, 1.0µm Rtx®-1 (cat.# 10159)1.0µL split injection of a 99.9% pure Freon® 114 standard.


Inj. & det. temp.: 250°CDet.: full scanCarrier gas: heliumLinear velocity: 20cm/sec. set @ 35°CSplit vent: 30mL/min.

1. Freon® 1152. Freon® 223. Freon® 124. Freon® 1245. Freon® 1146. Freon® 1112a7. Freon® 1238. Freon® 123a9. Freon® 11

10. Freon® 11311. Freon® 12212. Freon® 11213. Freon® 1110

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731Chlorofluorocarbons



1

2

3

4

5

6

min. 20 22 24 26 28 30GC_CH00321

1. Freon® 212. Freon® 1233. Freon® 123a4. Freon® 115. dichloromethane6. Freon® 113

105m, 0.53mm ID, 3.0µm Rtx®-1 (cat.# 10189)1.0µL splitless injection of a 500µg/mL mix of Freon® compounds

Splitless hold: 0.5 min.Oven temp.: 35°CInj. temp.: 220°CCarrier gas: heliumLinear velocity: 20cm/sec. set @ 35°CMSD: TIC modeScan rate: 1.5 scans/sec.


1

2

3

4

5

6

min. 12 13 14 15 16 17 18GC_CH00322

1. Freon® 212. Freon® 113. Freon® 1234. Freon® 123a5. Freon® 1136. dichloromethane

105m, 0.53mm ID, 3.0µm Rtx®-200 (cat.# 15091)1.0µL splitless injection of a 500µg/mL mix of Freon® compounds

Splitless hold: 0.5 min.Oven temp.: 35°CInj. temp.: 220°CCarrier gas: heliumLinear velocity: 20cm/sec. set @ 35°CMSD: TIC modeScan rate: 1.5 scans/sec.

Chlorofluorocarbons5% Krytox on 60/80 CarboBlack™ B

1

2

3

4

5 6

min. 4 8 12 16

1. Freon® 222. Freon® 123. Freon® 214. Freon® 1145. Freon® 114A isomer6. Freon® 11

5% Krytox on 60/80 CarboBlack™ B (cat.# 80127)3.05m, 1/8" OD x 2.1mm ID SilcoSmooth™ stainless steel tubing1µL injected, concentration 20% each component

Oven temp.: 50°CInj./det. temp.: 200°C/250°CCarrier gas: nitrogen @ 30mL/min.Detector: FID

GC_CH00323

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S732 Chlorofluorocarbons; Cresylic Acids


Freon® & Ethylene OxideRtx®-1

21. Freon® 122. ethylene oxide

30m, 0.53mm ID, 5.0µm Rtx®-1 (cat.# 10179)50µL split injection of Freon® 12 and ETO

Oven temp.: 25°CInj. & det. temp.: 290°CCarrier gas: hydrogenLinear velocity: 40cm/sec. FID sensitivity: 4 x 10-11 AFSSplit vent: 40:1

GC_CH00324

min. 2

1 2

Freon® & Ethylene OxideRtx®-200

1

min. 4

1. Freon® 122. ethylene oxide

30m, 0.53mm ID, 3.0µm Rtx®-200 (cat.# 15085)50µL direct injection of Freon® 12 and ETO

Oven temp.: 25°CInj. & det. temp.: 290°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5.2cc/min.)

GC_CH00325

Cresylic AcidsStabilwax®-DA

30m, 0.25mm ID, 0.50µm Stabilwax®-DA (cat.# 11038)Wet needle split injection of cresylic acids

Oven temp.: 180°C (hold 2 min.) to 260°C @ 2°C/min.Inj. & det. temp.: 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 3.2cc/min.)FID sensitivity: 64 x 10-11 AFSSplit ratio: 40:1

1. 2,6-dimethylphenol2. o-cresol3. phenol4. 2,4,6-trimethylphenol5. 2,3,6-trimethylphenol6. o-ethylphenol7. 2,5-dimethylphenol8. p-cresol9. 2,4-dimethylphenol

10. m-cresol11. C3 phenol12. 2,3-xylenol13. C3 phenol14. C3 phenol15. C3 phenol16. p-ethylphenol17. 3,5-xylenol18. m-ethylphenol19. 3,4-xylenol

min. 1 2 3 4 5 6 7 8 9 10 11 12GC_CH00277

1

2

3,4

5

6

7,89

10

1211

14

16,17 18

19

13

15

705-737_GCAPP.qxd 11/4/2004 11:13 AM Page 732

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733Cresylic Acids; Esters


Cresylic Acids Di-isodecyl Phthalate

1. phenol2. o-cresol3. 2,6-xylenol4. p-cresol5. m-cresol6. o-ethylphenol7. 2,4-xylenol8. 2,5-xylenol9. 2,4,6-trimethylphenol

10. 2,3-xylenol11. p-ethylphenol12. m-ethylphenol13. 3,5-xylenol14. C3 phenol15. 3,4-xylenol16. C3 phenol17. 4-ethyl,2-methylphenol18. 5-ethyl,2-methylphenol

60m, 0.25mm ID, 0.20µm DIIDP column (cat.# 50016)Wet needle split injection of cresylic acids

Oven temp.: 130°CInj. & det. temp.: 130°CCarrier gas: hydrogenLinear velocity: 40cm/sec.FID sensitivity: 32 x 10-11 AFSSplit ratio: 25:1

min. 4 8 12 16 20 24 28 32 36 40

17

18

16

15

14

1312

11

10

9

1

2

3

4

5

6

8

7

GC_CH00278

EstersStabilwax®

1

2

34

5 6

7

8

9

10

11

12

13

min. 2 4 6 8 10 12 14 16 18 20 22 24 26

60m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10658)1.0µL split injection of esters


Inj. & det. temp.: 250°C/285°CDet.: MSCarrier gas: heliumLinear velocity: 50cm/sec. set @ 50°CSplit ratio: 7:1Ionization: EI

1. pentane2. heptane3. methyl formate4. methyl acetate5. methyl propionate6. methyl butyrate7. methyl valerate8. methyl hexanoate9. methyl octanoate

10. pentadecane11. methyl decanoate12. methyl benzoate13. nonadecane

GC_CH00314

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S734 Ethers


EthersRtx®-502.2

1. methyl ether2. pentane3. ethyl ether4. dimethoxymethane5. isopropyl ether6. butenal7. 1,3-dioxolane8. 1,2-dimethoxyethane9. heptane

10. tetrahydro-2-methyl furan11. allyl ether12. ethyl acrylate

13. 1,4-dioxane14. butyl ether15. bis(2-methoxyethyl)ether16. 2-ethoxyethyl ether17. 1,2-bis(2-methoxyethoxy)ethane

or triethylene glycol dimethyl ether18. pentadecane19. bis(2-(2-methoxyethoxy)ethyl ether

or tetraethylene glycol dimethyl ether20. benzyl ether21. nonadecane

1

23

4 5

6

7

8

10

1211

13

14

1516

18

17

19

20

21

min. 10 20 30 40 50 60 70GC_CH00317

105m, 0.53mm ID, 3.0µm Rtx®-502.2 (cat.# 10910)1.0µL split injection of ethers


Inj. / det. temp.: 250°C/280°C Det.: MSCarrier gas: heliumLinear velocity: 50cm/sec. set @ 50°CSplit ratio: 7:1Ionization: EI

9

EthersStabilwax®

60m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10658)1.0µL split injection of ethers.


Inj. / det. temp.: 250°C/280°CDet.: MSCarrier gas: heliumLinear velocity: 50cm/sec. set @ 50°CSplit ratio: 7:1Ionization: EI

min. 5 10 15 20 25 30 35 40 45 50 55 60

1

23

45 6

7 8

9

10

11

12

13

GC_CH00318

1. tetrahydro-2-methyl furan2. butyraldehyde3. butyl ether4. 1,3-dioxolane5. ethyl acrylate6. 1,4-dioxane7. 2-methoxy ethyl ether8. 2-ethoxy ethyl ether9. pentadecane

10. 1,2-bis(2-methoxyethoxy)ethane or triethylene glycol dimethylether

11. nonadecane12. bis(2-(2-methoxyethoxy)ethyl) ether or

tetraethylene glycol dimethyl ether13. benzyl ether

705-737_GCAPP.qxd 11/4/2004 11:14 AM Page 734

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735Glycols; Ketones


GlycolsRtx®-200

1

23

4

1. ethylene glycol2. propylene glycol3. diethylene glycol4. tetraethylene glycol

min. 4 8 12 16 20 24 28GC_CH00326

60m, 0.53mm ID, 3.0µm Rtx®-200 (cat.# 15088)1.0µL split injection of a glycol sampleConc.: 50ng on-column

Oven temp.: 40°C to 250°C @ 8°C/min. (hold 15 min.)Inj. / det. temp.: 200°C / 250°CCarrier gas: hydrogenLinear velocity: 40cm/sec. (flow rate: 5cc/min.)FID sensitivity: 6.4 x 10-11 AFSSplit ratio: 19:1

Glycols & AlcoholsXTI®-5

1. n-butyl alcohol2. ethylene glycol3. propylene glycol4. butyl Cellosolve®

5. butyl carbitol

2

3

1 4

5

GC_CH00327

min. 4 8

30m, 0.53mm ID, 1.0µm XTI®-5 (cat.# 12255)1.0µL direct injection of glycols and alcoholsConc.: 100ppm

Oven temp.: 40°C to 185°C @ 15°C/min. (hold 5 min.)Inj. / det. temp.: 150°C / 200°CCarrier gas: heliumLinear velocity: 40cm/sec. (flow rate: 5cc/min.)FID sensitivity: 8 x 10-11 AFS

KetonesStabilwax®

60m, 0.53mm ID, 1.0µm Stabilwax® (cat.# 10658)1.0µL split injection of ketones

Oven temp.: 45°C (hold 10 min.) to 250°C @ 12°C/min.(hold 20 min.)

Inj. & det. temp.: 250°C Det.: MSDCarrier gas: heliumLinear velocity: 51cm/sec. set @ 50°CSplit ratio: 8:1Ionization: EI

1. 2,4-dimethyl-3-pentanone2. 4-methyl-2-pentanone3. 4-heptanone4. 4-methyl-3-penten-2-one5. 5-methyl-2-hexanone6. 5-methyl-3-heptanone7. 4-hexen-3-one8. 1-pentanol9. 4-hydroxy-4-methyl-2-pentanone

10. butyrolactone11. nonadecane

12

9

10

765

3

4

min. 5 10 15 20 25GC_CH00316

8

11 Searching for a chromatogram?

www.restek.com

705-737_GCAPP.qxd 11/4/2004 11:14 AM Page 735

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S736 Silanes; Styrene Impurities


SilanesRtx®-200

min. 4 8 12 16 20GC_CH00361

1. phenyldichlorosilane2. phenyltrichlorosilane3. methylphenyl dichlorosilane4. phenylvinyl dichlorosilane5. diphenyl dichlorosilane

5

4

3

21

60m, 0.53mm ID, 3.0µm Rtx®-200 (cat.# 15088)0.5µL split injection of phenyl silanes


Inj. / det. temp.: 200°C / 270°CCarrier gas: hydrogenLinear velocity: 40cm/sec.FID sensitivity: 1.02 x 10-9 AFSSplit vent: 40cc/min.

SilanesRtx®-200

4

5

3

2

1

min. 2 4 6

GC_CH00362

1. dimethylchlorosilane2. methyldichlorosilane3. trimethylchlorosilane4. methyltrichlorosilane5. dimethyldichlorosilane

60m, 0.53mm ID, 3.0µm Rtx®-200 (cat.# 15088)0.5µL split injection of methyl silanes


Inj. / det. temp.: 200°C / 270°CCarrier gas: hydrogenLinear velocity: 40cm/sec.FID sensitivity: 1.02 x 10-9 AFSSplit vent: 40cc/min.

Styrene ImpuritiesStabilwax®

1. butene2. 1,3-butadiene3. diethylhydroxylamine4. acrylonitrile5. vinylcyclohexene6. toluene7. ethylbenzene8. m-xylene9. cumene

10. o-xylene11. styrene

min. 4 8 12 16 20 24 28

10

9

8

7

6

5 11

34

1

2

GC_CH00356

Permission to publish this chromatogram granted by Copolymer Rubber and Chemical Corp.

30m, 0.53mm ID, 0.50µm Stabilwax® (cat.# 10640)0.5µL split injection of a 95% pure styrene


Inj. & det. temp.: 150°CCarrier gas: heliumLinear velocity: 20cm/sec. set @ 40°CFID sensitivity: 16 x 10-11 AFSSplit vent: 40cc/min.

Styrene ImpuritiesRtx®-1701

Permission to publish this chromatogram granted by Copolymer Rubber and Chemical Corp.

30m, 0.53mm ID, 3.0µm Rtx®-1701 (cat.# 12085)0.5mL split injection of a 95% pure styrene


Inj. & det. temp.: 150°CCarrier gas: heliumLinear velocity: 20cm/sec. set @ 40°CFID sensitivity: 16 x 10-11 AFSSplit vent: 40cc/min.

8

min. 4 8 12 16 20 24 28

3 4

9

7

106

11

5

2

1

GC_CH00357

1. 1,3-butadiene2. butene3. acrylonitrile4. diethylhydroxylamine5. toluene6. vinylcyclohexene7. ethylbenzene8. m-xylene9. o-xylene

10. styrene11. cumene

705-737_GCAPP.qxd 11/4/2004 11:14 AM Page 736

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737Siloxanes

www.restek.com

Siloxane (Polysiloxane 20) MXT®-5

min. 4 8 12 16 20 24 28 32

GC_CH00359

15m, 0.28mm ID, 0.25µm MXT®-5 (cat.# 70221)1.0µL split injection of diluted polysiloxane 20 in pentane

Oven temp.: 75°C to 340°C @ 15°C/min.Inj. / det. temp.: 250°C / 340°CCarrier gas: hydrogenLinear velocity: 40cm/sec.FID sensitivity: 16 x 10-11 AFSSplit ratio: 100:1

Siloxane, Polymethyl Rtx®-200

15m, 0.25mm ID, 0.25µm Rtx®-200 (cat.# 15020)1.0µL split injection of polymethyl siloxane

Oven temp.: 40°C to 360°C @ 15°C/min. (hold 30 min.)Inj. / det. temp.: 250°C / 360°CCarrier gas: hydrogenLinear velocity: 40cm/sec.FID sensitivity: 5.12 x 10-10 AFSSplit vent: 40cc/min.

GC_CH00360min. 2 4 6 8 10 12 14 16 18 20 22

705-737_GCAPP.qxd 11/12/2004 3:08 PM Page 737

460-737

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