Instrumentation, Assay and Equipment Design

4960691

C H R O M A T O G R A P H I C T E S T S T R I P F O R D E T E R M I N I N G L I G A N D S O R R E C E P T O R S

Julian Gordon, Michael E McMahon, Shanfun Ching assigned to Abbott Laboratories

A test strip for analysis of analytes such as anti- gens, antibodies or polynudeotides employs a chromatographic medium and a solvent capable of transporting reagents and/or sample. Rea- gents are selected and disposed on the medium such that a labeled (first) reagent arrives at the detection (third) zone only after analyte is im- mobilized there and non-reactive sample com- ponets have been transported beyond the detection zone. This sequential arrival is ac- complished by the relative mobility of the rea- gent or sample; or by the site relationship of the zones. Preferably, the site relationship of the sample (second) zone and the label (first) zone is such that a plurality of pathways guide the sam- ple and labeled reagent and any subsequent rea- gents to the detection zone in the recited order. Single and multiple pathway devices are dis- dosed.

4960692

A S S A Y E M P L O Y I N G B I N D I N G P A I R M E M B E R S O N P A R T I C L E S

A N D O N A F I L T E R O R M E M B R A N E

Brian B Lentrichia, Michael F Turanchik as- signed to Fisher Scientific Company

An assay method and kit in which particles bearing a first reagent binding pair member (e.g.,

anti-digoxin antibody) react with a sample such that analyte binding pair member (e.g., digoxin) binds to the first reagent binding pair member. The reaction mixture is then passed through a fil- ter or membrane or pore size sufficient to allow particles to pass through. A second reagent bi- nding pair member (e.g., dignxin-albumin con- jugate) is immobilized on the filter or membrane to trap preferentially either particles which have bound analyte binding pair members or particles which have not, leaving the other class of par- ticles to pass through the filter for detection by resistive pulse techniques, by light absorbence or scattering, by enzymatic read-out (when the par- ticles are enzyme-labeled) or otherwise.

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4960693

S Y S T E M O F R E A C T A N T S I N V O L V I N G A N A P O - E N Z Y M E U S E F U L I N I M M U N O L O G I C A L

A N A L Y S I S A N D A M E T H O D F O R C A R R Y I N G O U T I M M U O A S S A Y S

W I T H T H I S S Y S T E M

Iqbal Siddiqi, Ciaron Mangan, Geneva, Switzer- land assigned to Intracel Corporation

A quick and simple immunoassay technique applicable to the determination of large molecules like proteins, polynucleotides and others involves labelling competitive immuno- species with an enzyme as signal generator. This enzyme can be converted to the corresponding apt-enzyme form and its regeneration, upon ad- dition of a suitable co-factor, is inhibited by complexation when the labelled specie is reacted with its immunoparmer. The degree of com- plexation is therefore ascertained by measuring the extent of regenerated activity.