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5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25

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Page 1: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

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The Clone Age

Human GenomeProject

Recombinant

DNA

Gel Electrophoresis

DNA fingerprints

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Page 2: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

The first adult mammal successfully cloned was a sheep named this.

What is Dolly?

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Page 3: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

Dolly was cloned from what type of cell.

What is a mammary cell?

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Page 4: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

These are the two most common DNA cloning vectors.

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What are plasmids and

bacteriophages?

Page 5: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

Dolly was created using this type of cloning

technique.

What is nuclear transfer?

2020

Page 6: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

Cloned goats are used to produce this substance

for human use.

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What is clotting factor?

Page 7: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

The Human Genome Project was completed in this year.

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What is 2003?

Page 8: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

The Human Genome Project discovered that humans have this many

genes.

What is 30,000 to 40,000?

1010

Page 9: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

This is an organism’s complete set of proteins.

What is a proteome?

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Page 10: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

This percentage of human DNA differs from person to person.

What is 0.1%?

2020

Page 11: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

The Human Genome Project revealed 8 million loci where individuals differ by only one nucleotide. These are known

as this.

What are SNPS (single nucleotide

polymorphisms?

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Page 12: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

These are enzymes that cut DNA molecules at specific places.

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What are restriction Enzymes?

Page 13: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

These are circular pieces of bacterial DNA that replicate and can be used

as recombinant DNA vectors in genetic engineering.

What are plasmids?

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Page 14: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

Fragments of DNA can join with each other if they have

complementary sequences of bases on their ends known as this.

What are sticky ends?

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Page 15: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

This human protein needed by diabetics is produced through recombinant DNA technology.

What is insulin?

2020

Page 16: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

Recombinant DNA means that the DNA from two different ones of

these have been combined.

What is species?

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Page 17: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

What is size?

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Gel Electrophoresis sorts DNA fragments

according to this.

Page 18: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

Gel Electrophoresis makes use of the fact that DNA

molecules have this overall charge.

What is negative?

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Page 19: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

DNA will move towards what end of the chamber?

What is the positive end?

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Page 20: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

The gel in gel electrophoresis is made

of this substance.

What is agarose?

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Page 21: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

This is the name of the solution that is poured into

the chamber.

What is TBE buffer?

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Page 22: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

This is the first step in DNA fingerprinting?

What is isolate a DNA sample?

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Page 23: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

If a sample of DNA found at a crime scene is very small, this technique could be used to amplify (or make

multiple copies) of it.

What is PCR (Polymerase Chain Reaction)?

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Page 24: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

DNA fingerprinting makes use of these segments of

repeating DNA nucleotides?

What is VNTRs (Variable number tandem repeats)?

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Page 25: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

In DNA fingerprinting, cut DNA fragments are sorted

using this technology.

What is Gel Electrophoresis?

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Page 26: 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15

The FBI uses this many VNTRs to make a positive

DNA match.

What is 13?

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