5-day cidr synch

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E10890 Penny Lane • Baraboo, WI 53913 800.451.9275 • 608.356.8357 [email protected]www.accel gen.com Dr. Humberto Rivera, M.S.  hrivera@acce lgen.com Dr. Alex Souza, Ph.D. [email protected] REPRODUCTIVE SPECIALISTS VOLUME 2 ISSUE 1  Al th ou gh pr ograms combinin g prostaglandin (PGF2 ) treatments and heat detection are very effective for dairy heifers, many producers have to rely on Timed A.I. protocols to get their heifers bred due to a number of different reasons. Unfortunately , when the original Ovsynch protocol is used in heifers, conception rates are – in general – much lower than conception results achieved with breedings performed following estrus detection. These lower results with Ovsynch in heifers seem to be related with faster follicular turn-over, lower ovulation rates, and higher percentage of heifers displaying premature estrus before scheduled A.I. (revised by Rivera et al., 2004). Most research groups tend to accept the fact that regular CIDR- synch-like protocols can efficiently reduce amount of heifers showing premature estrus during timed A.I. procedures; yet, conception rates don’t seem to be ideal, and most frequently inferior to conception results attained with breedings following estrus detection. In some countries such as Argentina and Brazil, synchronization protocols that combine estrogens with short half-life and progesterone devices, with or without equine gonadotropin (eCG) applied at the end of the protocol, are very popular and seem to produce more acceptable results in terms of conception in dairy and beef nuliparous heifers. Unfortunately, estrogens and equine gonadotropin are not available for synchronization purposes for cattle in U.S. Thus, this 5-DAY CIDR SYNCH: THE MISSING PROTOCOL FOR DAIRY HEIFERS insider   ARTICLE BY : DR . A LEX  S OUZA, P H . D. R EPRODUCTIVE S PECIALIST * A single PGF treatment at CIDR removal seems to be enough in heifers. However, a second Chart 1. 5-Day CIDR Synch Protocol. (Source: Bridges et al., 2008)

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Page 1: 5-Day Cidr Synch

8/13/2019 5-Day Cidr Synch

http://slidepdf.com/reader/full/5-day-cidr-synch 1/4

E10890 Penny Lane • Baraboo, WI 53913

800.451.9275 • 608.356.8357 

[email protected] • www.accelgen.com

Dr. Humberto Rivera, M.S.

 [email protected]

Dr. Alex Souza, Ph.D.

[email protected]

REPRODUCTIVE SPECIALISTS 

VOLUME 2 ISSUE

 Although programs combiningprostaglandin (PGF2

) treatments and

heat detection are very effective fordairy heifers, many producers have to

rely on Timed A.I. protocols to get theirheifers bred due to a number of differentreasons. Unfortunately, when the originalOvsynch protocol is used in heifers,conception rates are – in general – muchlower than conception results achievedwith breedings performed followingestrus detection. These lower results withOvsynch in heifers seem to be related

with faster follicular turn-over, lowerovulation rates, and higher percentageof heifers displaying premature estrusbefore scheduled A.I. (revised by Rivera

et al., 2004). Most research groups tendto accept the fact that regular CIDR-synch-like protocols can efficiently reduceamount of heifers showing prematureestrus during timed A.I. procedures; yet,conception rates don’t seem to be ideal,and most frequently inferior to conceptionresults attained with breedings followingestrus detection.

In some countries such as Argentinand Brazil, synchronization protocothat combine estrogens with shohalf-life and progesterone device

with or without equine gonadotrop(eCG) applied at the end of thprotocol, are very popular and seeto produce more acceptable results terms of conception in dairy and benuliparous heifers. Unfortunateestrogens and equine gonadotropare not available for synchronizatiopurposes for cattle in U.S. Thus, th

5-DAY CIDR SYNCH: THE MISSING

PROTOCOL FOR DAIRY HEIFERS

™ insider  ARTICLE BY:

DR . ALEX  S OUZA, P H . D.

R EPRODUCTIVE 

S PECIALIST 

* A single PGF treatment at CIDR removal seems to be enough in heifers. However, a second

Chart 1. 5-Day CIDR Synch Protocol. (Source: Bridges et al., 200

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™ insider 

article will try to present some insightabout recent and promising findings

towards developing an efficientsynchronization program for heifersutilizing hormones that are availablein the U.S. market.

Currently, one of the best protocolsfor dairy heifers in terms of conceptionseems to be the newly developed5-Day CIDR Synch (Bridges etal., 2008). This is the sequence of

injection during the 5-Day CIDRprotocol: on Day 0, CIDR is appliedtogether with a GnRH injection. Fivedays later (5 complete days afterCIDR insertion!), CIDR is removedand a PGF2

  treatment is given at

the same time. A second PGF2  is

required in beef and dairy cows (butnot on heifers), sometime between8 to 24 hours after CIDR removal.

Then, three days after CIDR removal

(Day 8), animals are bred togetherwith an additional GnRH treatment

at the time of A.I.

The original work to develop the5-Day CIDR protocol was done byBridges et al. (2008) using beefcows as experimental units. Theirresults suggested that reducingCIDR insertion from 7 to 5 days,associated with longer pro-estrusintervals improved conception

rates. It is important to highlightthat Bridges et al. (2008) used twoPGF2

  injections in all trials to

enhance CL regression, the firstPGF2

 was done at CIDR removal

and the second PGF2  was given

12 hours later.

This pioneer work was done by Bridgeset al (2008) and served as rationale

for several further trials using beef

(Kasimanickam, et al., 2009), dacows (Chebel et al., 2009), and daheifers (preliminary results show

by Dr. Thatcher at DCRC meetingMinneapolis, MN 2009). In addition,number of other ongoing experimenare to be published in the next coupof months, and they will provide moknowledge about the use and efficaof the 5-Day CIDR protocol.

For instance, Kasimanickam et a(2009) tested whether two PGF2treatments are actually needed CIDR removal when a 5-Day protocis used in beef cows. Secondarily, this same trial, they compared twtypes of PGF products (dinoprovs cloprostenol) when a single PGtreatment was given at CIDR removaIn summary, they found no differencbetween the two PGF2

  produc

However, two PGF2  treatmen

seem to be necessary for maximu

conception rates in beef cowreceiving the 5-Day CIDR protocol.

Finally, there is sufficient scientievidence proving the effectiveneof the 5-Day CIDR protocol dairy heifers (data summarizeand presented by Dr. Thatcher DCRC meeting in Minneapolis, M

 – 2009). Briefly, these researche

observed in a series of field triathat a single PGF2

  treatme

given at CIDR removal is enough produce good CL regression, anoutstanding conception results thare comparable to conception resuachieved when heifers are bred aftregular estrus detection.

Dr. Thatcher also reported simil

conception rates in heifers treate

Table 1. Type of CIDR treatment and fertility of beef cows.

Experimental Groups

(Source: Adapted from Bridges et al., 2008; Exp. 4) 

    C    R    (    %    )

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™ insider with new (59.7%) vs 5-day useCIDRs (61.2%). Obviously, correhandling, sanitization, and ev

autoclaving are important detawhen re-utilizing CIDRs, mostly, avoid horizontal spread of infectiodiseases within the herd.

The new 5-Day CIDR Synch migalso be a good option for those wwant to use sexed semen associatewith Timed A.I. in heifers. In faaccording to later findings (DCR

2009), the 5-Day CIDR protocproduced acceptable conceptiresults even after the use of sexesemen in heifers. For example, in oof the trials that they used the 5-DCIDR protocol with a single PGFat CIDR removal, they found thconception rates for dairy heifers brewith sexed semen was 43% (43/10compared to 53.1% (52/98) for heifeinseminated with regular semen. Thuconception results with sexed semin heifers treated with the 5-Day CIDSynch are in the same range of tresults achieved when sexed semis used after estrus detection, bfurther trials are necessary to confithese encouraging findings.

In Summary, here are some of tadvantages of the 5-Day CIDR Syn

program:

1) No pre-synchronization is needed

2) Minimum amount of handlings – very simple to apply.

3) Consistent conception results ancomparable fertility to heat detectiobreedings, even in heifers.

Table 2. Type A & D Single vs. Double PGF in Beef Cows.

Table 3. Effect of One vs. Two PGF in Dairy Heifers.

Experimental Groups

(Source: Adapted from Kasimanickam et al., 2008) 

    C    R    (    %    )

Experimental Groups

(Source: Adapted from Thatcher et al., 2009 - DCRC) 

    C    R    (    %    )

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™ insider 

 Accelerated Genetics • 800.451.9275 • 608.356.8357 • [email protected] • www.accelgen.com

   P   R   S   R   T   S   T   D

   U .   S .   P   O   S   T   A   G   E

     P      A  I     D

   M   A   D I   S   O   N ,    W I

   P   E   R   M I   T   N   O .  1  9  49

  0   P  e  n  n  y  L  a  n  e .   B  a  r  a  b  o  o ,    W I  5  3  9  1  3  -  9  4  0  8

  e  s  s   S  e  r  v i  c  e   R  e  q  u  e  s  t  e  d

4) The entire protocol lasts only 8days, which makes it an attractiveoption for re-synch breedings for

heifers, as well as for beef and dairycows (future research needs tovalidate this for dairy cows).

5) 5-Day CIDR synch providesacceptable results following sexedsemen breedings in heifers.

REFERENCES:Decreasing the interval betweenthe GnRH and PGF2

  from 7 to 5 d

and lengthening proestrus increasestimed-AI PR in beef cows. Bridgeset al., 2008. Theriogenology 69:843-851.

Effect of reducing the period of follicledominance in a timed AI protocol onreproduction of dairy cows. Chebel.,et al. 2009. Proceedings ADSA-ASAS;pg. 250-251; Indianapolis, IN.

Two doses of prostaglandin improvepregnancy rates to timed-AI in a 5-dprogesterone-based synchronization

protocol in beef cows. Kasimanickaet al., 2009. Theriogenology 71:76767.

Timed AI programs in dairy heifeThatcher et al., 2009. ProceedinDCRC; pg. 57-70; Minneapolis, MN

Fertility of Holstein dairy heifers afsynchronization of ovulation and tim

 AI or AI after removed tail chalk. Riveet al., 2004. J. Dairy Sci. 87:2052061.

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