6850/6890 Split AnimationSplit/Splitless Inlet - Component IdentificationSeptumLinerLiner O-RingGold SealCapillary Column5/4/00

6850/90 Split/Splitless Inlet - Component IdentificationSeptumSEPTUM PURGEVENTInlet SplitPort

6850/90 Split Animation - Default ConditionsFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORPURGEVALVEOPENTOTAL FLOWCONTROL LOOPSPLITVENTGOLD SEALINPUTPRESSUREPROPORTIONALVALVE 280 PSI0

ML/MINSPLIT VENTTRAP0

PSI

6850/90 Split Animation - Total Flow SettingFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORPURGEVALVEOPENTOTAL FLOWCONTROL LOOPSPLITVENT

205 ML/MINGOLD SEALINPUTPRESSUREPROPORTIONALVALVE 280 PSIPRIMARY FLOW PATHREDUCED FLOW PATH205

ML/MINSPLIT VENTTRAP0

PSI

6850/90 Split Animation - Inlet Pressure SettingFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPGOLD SEALINPUTPRESSUREPROPORTIONALVALVE 280 PSIPRIMARY FLOW PATHREDUCED FLOW PATH205

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

200 ML/MINCOLUMN FLOW2 ML/MINPRESSURIZED GASBACK PRESSURE CONTROL LOOP

6850/90 Split Ready for Injection AnimationFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPINPUTPRESSUREPROPORTIONALVALVE 280 PSI205

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

200 ML/MINCOLUMN FLOW2 ML/MINBACK PRESSURE CONTROL LOOPSplit Ratio - 100:1Mode:SplitTemp 250250Pressure 10.010.0Split Ratio 100 Split Flow 200Tot Flow 205.0205.0Gas SaverOFF

6850/90 Split Insert Syringe AnimationFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPGOLD SEALINPUTPRESSUREPROPORTIONALVALVE 280 PSI205

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

200 ML/MINCOLUMN FLOW2 ML/MINBACK PRESSURE CONTROL LOOPSplit Ratio - 100:1

6850/90 Split Inject Sample AnimationFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPGOLD SEALINPUTPRESSUREPROPORTIONALVALVE 280 PSI205

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

200 ML/MINCOLUMN FLOW2 ML/MINBACK PRESSURE CONTROL LOOPSplit Ratio - 100:1

6850/90 Split Sample Vaporizes AnimationFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPGOLD SEALINPUTPRESSUREPROPORTIONALVALVE 280 PSI205

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

200 ML/MINCOLUMN FLOW2 ML/MINBACK PRESSURE CONTROL LOOPSplit Ratio - 100:1

6850/90 Split Sample is Split - Animation Good Liner Design?FLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPINPUTPRESSUREPROPORTIONALVALVE 280 PSI205

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

200 ML/MINCOLUMN FLOW2 ML/MINBACK PRESSURE CONTROL LOOPSplit Ratio - 100:1Agilent SplitLiner - 5183-4647

6850/90 Split Split Continues AnimationFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPINPUTPRESSUREPROPORTIONALVALVE 280 PSI205

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

200 ML/MINCOLUMN FLOW2 ML/MINBACK PRESSURE CONTROL LOOPSplit Ratio - 100:1

6850/90 Split Split is Complete AnimationFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPINPUTPRESSUREPROPORTIONALVALVE 280 PSI205

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

200 ML/MINCOLUMN FLOW2 ML/MINBACK PRESSURE CONTROL LOOPSplit Ratio - 100:1

6850/90 Split Sample is separated Animation and DetectedFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPINPUTPRESSUREPROPORTIONALVALVE 280 PSI205

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

200 ML/MINCOLUMN FLOW2 ML/MINBACK PRESSURE CONTROL LOOPSplit Ratio - 100:1

6850/90 Split Gas Saver - Set to 55 ml/min Animation After the Injection is completeFLOWSENSORPROPORTIONALVALVE 1PRESSURESENSORSEPTUM PURGEREGULATORFIXED @ 3ML/MINPURGEVALVEOPENTOTAL FLOWCONTROL LOOPINPUTPRESSUREPROPORTIONALVALVE 280 PSI55

ML/MINSPLIT VENTTRAP10

PSISPLITVENT

50 ML/MINCOLUMN FLOW2 ML/MINBACK PRESSURE CONTROL LOOPSplit Ratio - 100:1Mode:SplitTemp 250250Pressure 10.010.0Split Ratio 100 Split Flow 200Tot Flow 205.0205.0Gas SaverONSaver Flow50.0Saver Time0.5

Split Splitless Inlet Component Identification:

The drawing on the left will be used to demonstrate Split operation of the Agilent 6850 and 6890 Split/Splitless Capillary Inlet. The internal components in the injection port are as follows:

1) Septum2) Inlet Liner3) Liner O-Ring seal4) Gold Inlet seal5) Capillary ColumnSplit Splitless Inlet Component Identification:

The components external to the inlet injection port will also be identified as follows:

1) Carrier gas supply connection2) EPC Proportional Valve 13) Flow Sensor4) Septum5) Inlet Split vent6) Split vent trap7) Pressure sensor8) Purge on/off valve9) EPC Proportional Valve 210) Septum Purge Regulator11) Septum Purge Vent12) Split Vent

The Capillary Inlet is shown in Split Mode with no flow or pressure setpoints entered. The Inlet Total Flow is set to 205 ml/minute. Proportional valve 1 opens to introduce flow, and then controls at the setpoint. The flow path is through the inlet liner, around the outside of the liner and out the split vent through the filter, Purge Valve and Proportional Valve 2. Proportional valve 2 is completely open until a pressure setpoint is entered.An Inlet pressure of 10 PSI is entered. 10 PSI is the pressure required to establish 2 ml/minute of flow on this particular column at the oven starting temperature. Proportional valve 2 starts to close off, increasing Inlet back pressure, then controlling at 10 PSI. Once the inlet pressurizes, the septum purge and column flows are established. The Total flow is equal to the sum of Column + Split Vent + Septum Purge flows. The Capillary Inlet is Ready for Injection. The split ratio is equal to the split vent flow rate divided by the column flow rate - in this example 100:1.The sample syringe is inserted into the injection port.The plunger is depressed, injecting liquid sample into the inlet.The sample is vaporized. It is important to be sure that the inlet liner has sufficient internal volume to contain the solvent vapor. The amount of vapor will depend on the solvent used, the injection volume, and the inlet temperature and pressure.

Because of the very short sample residence time in the inlet it is beneficial to use an inlet liner that has some glass wool inserted to provide adequate surface area for complete sample vaporization and mixing. This will help minimize boiling point discrimination during the split process.The recommended split liner is shown above. This liner incorporate the following design features:

1) Glass wool held in position by glass indents to provide mixing and to wipe the syringe needle after the injection.

2) The O.D. is slightly smaller than splitless liners to provide an unrestricted path for the split flow.

3) A taper at the bottom of the inlet to direct sample to the column.

4) A glass positioning bead to provide clearance between the bottom of the liner and the gold seal. This further reduces split flow restriction, improving split precision.

5) The liner is also deactivated.The entire split process in the inlet occurs in a fraction of a second. In this example if the liner has an internal volume of 1 ml, then the split occurs in 1/200 of a minute.In this example if 1 micro liter of sample was injected, .01 ul went onto the GC column.Once the split is complete the sample is separated on the GC column, detected and the chromatogram is completed.A useful and cost saving feature of the 6850 and 6890 GCs is gas saver. The gas saver can be turned on during the GC run any time after the injection split is completed. The gas saver total flow is lower than the total flow during injection. The total flow stays low during the rest of the run and between runs, thus saving large amounts of carrier gas and money.