9 7 2 r 3 r i 6 h s c s 7 c e 5 n y e n y g r d t a g r d ... · phenomenex l web: t:m f: australia...
TRANSCRIPT
Phenomenex l WEB: www.phenomenex.com
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www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]
Terms and Conditions*Offer applies only to Phenomenex products listed in this brochure. Offer valid only at time of purchase and may not be combined with any other discounts or promotional items. Limit 10 columns per customer. Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.
Trademarks Luna is a registered trademark of Phenomenex, Inc. Ultremex and SecurityGuard are trademarks of Phenomenex, Inc. Ultrasphere is a registered trademark of Beckman Coulter, Inc.
Disclaimer Comparative separations may not be representative of all applications. Phenomenex is not affiliated with Beckman Coulter, Inc.
© 2010 Phenomenex. All rights reserved.
Ordering Information
2Phenomenex® offers two great solutions.
411
Mad
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ance
, CA
905
01-1
430
US
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BR
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100
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4
1. Direct Replacement: Ultremex™
2. Recommended Alternative: Luna®
Ultrasphere Guards Phenomenex Guard Replacement◊
Phase Part No. Part No.Ultrasphere Cyano (CN) 244072 AJ0-4305Ultrasphere ODS (C18) 243533 AJ0-4287Ultrasphere Octyl (C8) 243532 AJ0-4290Ultrasphere ODS Ion Pair 243534 AJ0-4287Ultrasphere Si (Silica) 244011 AJ0-4348
Guard Holder Guard Holder
Holder Assembly, XL Columns 237060 KJ0-4282◊Protects 3.2-8.0 mm ID columns
Upgrade to SecurityGuard™
Protects all your HPLC columns Easy to use Inexpensive
Are you using Ultrasphere® Guards?
Ultremex Columns (2.0 or 4.6 mm ID only)
Ultrasphere®
Columns will not be manufactured by Beckman Coulter® from January 1, 2010.
You asked for alternatives.We listened...
Ordering Information and Cross-Reference ChartUltrasphere® Phenomenex Luna®
Description (mm) Part No. Part No.3 µmC8 75 x 4.6 244228 00C-4248-E0C18 75 x 4.6 244254 00C-4251-E0CN 75 x 4.6 237573 00C-4254-E05 µm C8 150 x 4.6 235333 00F-4040-E0C8 250 x 4.6 235332 00G-4040-E0C18 150 x 2.0 237390 00F-4252-B0C18 150 x 4.6 235330 00F-4252-E0C18 250 x 4.6 235329 00G-4252-E0C18 IP 250 x 4.6 235335 00G-4252-E0C18 250 x 10 235328 00G-4252-N0Si 150 x 4.6 235342 00F-4274-E0Si 250 x 4.6 235341 00G-4274-E0CN 150 x 4.6 244070 00F-4255-E0CN 250 x 4.6 244071 00G-4255-E0
Ordering Information and Cross-Reference ChartUltrasphere® Phenomenex Ultremex™ Description (mm) Part No. Part No.3 µmC8 75 x 4.6 244228 00C-0038-E0C18 75 x 4.6 244254 00C-0039-E05 µm C8 150 x 4.6 235333 00F-0047-E0C8 250 x 4.6 235332 00G-0047-E0C18 150 x 2.0 237390 00F-0048-B0C18 150 x 4.6 235330 00F-0048-E0C18 250 x 4.6 235329 00G-0048-E0C18 IP 250 x 4.6 235335 00G-0048-E0C18 250 x 10 235328 00G-0048-N0
FREESecurityGuard™ Holderwith any purchase*
Mention offer code: ULT0710
Expires:
KJO-4282
SecurityGuard™ Holder with any purchase*
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tel.: (310) 212-0555 I fax: (310) 328-7768 I web: www.phenomenex.com I email: [email protected] l WEB: www.phenomenex.com Phenomenex l WEB: www.phenomenex.com
1Solution 1:
Direct Replacement: Ultremex™
Ultremex• ™ closely mimics the performance of UItrasphere®
Highly Reproducible•Long Column Life•Fully Guaranteed*•
Compare Selectivity
Compare physical and chemical specifications:
Phase Carbon Load Pore Size pH Range Endcapped Surface AreaUltrasphere C18 12 % 80 Å 2.5 to 7.5 yes 190 m2/g
Ultremex C18 13 % 80 Å 2.5 to 7.5 yes 200 m2/g
2Solution 2:
Recommended Alternative: Luna®
Compare Performance
Ultrasphere® Luna®
Conditions for both columns:Dimensions: 250 x 4.6 mm
Mobile Phase: 20 mM Phosphate Buffer, pH 7.5 /Methanol (65:35)
Flow Rate: 1 mL/minTemperature: 25 °C
Detection: UV @ 280 nmSample: 1. p-Aminobenzoic Acid
2. Procainamide
Conditions for both columns:Dimensions: 150 x 4.6 mm
Mobile Phase: A: 6.97 g/L Dipotassium Hydrogen Phosphate Buffer, pH 7.2
B: Acetonitrile Gradient: Time(min) % B Time(min) % B
0 37 35 6522 37 36 3727 65 50 37
Flow Rate: 2 mL/minTemperature: 25 °C
Detection: UV @ 278 nmSample: 1. Verapamil Hydrochloride CRS
2. Verapamil Hydrochloride Impurity I3. Verapamil Hydrochloride Impurity M
Ap
p ID
18
943
Ap
p ID
18
944
Very High Efficiencies•Excellent Peak Shape•Highly Reproducible•Fully Guaranteed**•
Ultrasphere® Ultremex™
Conditions for both columns:Dimensions: 250 x 4.6 mm
Mobile Phase: Water / Methanol (60:40)Flow Rate: 1 mL/min
Temperature: ambientDetection: UV @ 238 nm
Sample: 1. Bromacil2. Simazine3. Atrazine4. Diuron
Conditions for both columns:Dimensions: 150 x 4.6 mm
Mobile Phase: Acetonitrile / Water (65:35)Flow Rate: 1 mL/min
Temperature: 30 °CDetection: UV @ 210 nm
Sample: 1. Acetophenone2. Benzene3. Toluene4. Naphthalene
Ap
p ID
19
025
Ap
p ID
19
024
Phenomenex®
Choose the recognized Ultrasphere® alternatives. Two solutions available:
Luna® Column to Column ReproducibilityThe chromatograms on the right show consistency of inertness (black) and hydrophobicity (blue) for Luna 5 µm C18(2) columns from 9 different batches. Almost no variation is observed.
Column-to-column reproducibility for 9 batches of Luna
**If Luna does not provide you with at least equivalent separation to an Ultrasphere column of the same phase, particle size, and dimension, send in your comparative data within 45 days and keep the Luna column for FREE.
Comparative separations may not be representative of all applications.
*If Ultremex does not provide you with equivalent separation to an Ultrasphere column of the same phase, particle size, and dimension, send in your comparative data within 45 days and keep the Ultremex column for FREE.
Comparative separations may not be representative of all applications.
Inert TestHydrophobicity
Batch No.
1
2
3
4
5
6
7
8
9
VS
VS
Phenomenex®
BR71491009_I
To c
omp
lete
thi
s fo
rm o
nlin
e, v
isit
w
ww
.phe
nom
enex
.com
/info
/ultr
asp
here
Fax
+1
310-
328-
7768
or M
ail
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k Yo
u fo
r Yo
ur T
ime!
W
e ap
pre
ciat
e yo
ur b
usin
ess
and
wan
t to
mak
e su
re w
e m
eet
your
exp
ecta
tions
. Yo
ur fe
edb
ack
is v
ery
imp
orta
nt t
o us
.
FRE
E P
olo
shirt
whe
n yo
u co
mp
lete
all
que
stio
ns b
elow
ME
0 -7
897
© 2010 Phenomenex, Inc. All rights reserved.
Your
info
rmat
ion
Ans
wer
the
follo
win
g q
uest
ions
To s
ee a
dd
ition
al a
pp
licat
ions
for:
___
____
____
____
____
____
____
____
To s
et u
p a
free
eva
luat
ion
of a
Lun
a co
lum
n.To
dis
cuss
my
met
hod
, ple
ase
have
my
tech
nica
l con
sulta
nt c
onta
ct m
e.
Yes,
I am
inte
rest
ed in
lear
ning
mor
e ab
out
Luna
®, a
nd I
wou
ld li
ke:
Larg
eM
ediu
m
Sel
ect
Pol
o S
hirt
Siz
e:
1. A
re y
ou u
sing
Ultr
asp
here
® c
olum
ns?
Yes
(ple
ase
stat
e)
N
o
2. W
ould
you
be
inte
rest
ed in
:
A) D
irect
rep
lace
men
t fo
r U
ltras
phe
re
Ye
s
No
(ple
ase
exp
lain
) ___
____
____
____
____
____
____
____
B
) Alte
rnat
ive
colu
mn
to U
ltras
phe
re
Ye
s
No
(ple
ase
exp
lain
) ___
____
____
____
____
____
____
____
5 µm C18 5 µm C18(2)
Ordering Information on Page 4
1 2 3
Ordering Information on Page 4
Ap
p ID
18
918
0 10 20 30 min
0
2
4
6
8
mAU
18918
1 2
35 µm C8
Ap
p ID
18
917
0 10 20 30 min
0
2
4
6
8
mAU
18917
12
35 µm C8(2)
min0 1 2 3 4 5
mAU
0
100
200
300
400
500
19025
1
2
3
4
19024
min0 1 2 3 4 5
mAU
0
100
200
300
400
500
600
1
2
3
4
0 2 4 6 8 min
0
100
200
mAU
18943
1
2
0 2 4 6 8 min
0
100
200
mAU
18944
1
2
3. W
ould
you
eva
luat
e a
Luna
® c
olum
n as
an
alte
rnat
ive
to
Ultr
asp
here
?
Ye
s
N
o (p
leas
e ex
pla
in) _
____
____
____
____
____
____
____
__
020
40
mAU
1
2
3
4
19073
0 2 4 6 8 10 12 14 min
19072
020
40
mAU
1
2
3
4
0 2 4 6 8 10 12 14 min
Ap
p ID
19
073
Ap
p ID
19
072
Qty
/Yr
Col
umn
Siz
eP
hase
Sup
plie
rP
rice
(eac
h)
Need Re-Validation Assistance?We offer FREE Support!
How to move forward:For more information on these services or any of our service offerings, please call your local Technical Consultant, or visit us on the web at www.phenomenex.com/info/phenodev
5 µm C18 5 µm C18
5 µm C8 5 µm C8
Ult
rasp
her
e is
a r
egis
tere
d t
rad
emar
k o
f B
eckm
an C
ou
lter
, In
c.
*Depending on the complexity of a project, extended timelines and certain fees may be involved. These are determined at the start of a project.
Nam
e:
Com
pan
y:
Dep
artm
ent:
Com
pan
y A
dd
ress
:
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:S
tate
/Pro
v/C
ount
y:
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t C
ode:
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ntry
:
Tel:
Fax:
Em
ail:
Mr.
❑M
rs.
❑
Ms.
❑D
r.
❑
Pro
f.
❑
Y
es! P
leas
e se
nd m
e p
erio
dic
up
dat
es o
n ne
w t
echn
olog
ies
and
pro
duc
ts b
y em
ail.
____
____
____
____
____
____
____
___
____
____
____
____
____
____
____
___
____
____
____
____
____
____
____
___
Method development, optimization, and re-validation
services are available for your current Ultrasphere methods.
Simply give us a call, fill out a Project Data Sheet (PDS),
and mail us your sample (and current method if applicable).*
Phenomenex Chromatography Services