9. basic concepts of one way analysis of variance (anova)

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    Basic Concepts of One-wayAnalysis of Variance

    (ANOVA)

    Spori Goran, PhD.

    http://kif.hr/predmet/mki

    http://www.science4performance.com/

    http://kif.hr/predmet/mkihttp://kif.hr/predmet/mki
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    Overview What is ANOVA?

    When is it useful? How does it work? Some Examples

    Limitations Conclusions

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    Definitions ANOVA: analysis of variation in an

    experimental outcome and

    especially of a statistical variance inorder to determine the contributionsof given factors or variables to thevariance.

    Remember: Variance: the square ofthe standard deviation

    Remember: RA

    Fischer, 1919-

    Evolutionary Biology

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    Introduction Any data set has variability

    Variability exists within groups

    and between groups

    Question that ANOVA allows us toanswer : Is this variability significant, ormerely by chance?

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    The difference between variationwithin a group and variation

    between groups may help usdetermine this. If both are equal it islikely that it is due to chance andnot significant.

    H0: Variability w/i groups =variability b/t groups, this meansthat 1 = n

    Ha: Variability w/i groups does not =variability b/t groups, or, 1 n

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    Assumptions Normal distribution

    Variances of dependent variableare equal in all populations

    Random samples; independentscores

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    One-Way ANOVA One factor (manipulated

    variable)

    One response variable

    Two or more groups to compare

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    Usefulness Similar to t-test

    More versatile than t-test

    Compare one parameter(response variable) betweentwo or more groups

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    For instance, ANOVA

    Could be Used to: Compare heights of plants with andwithout galls

    Compare birth weights of deer indifferent geographical regions

    Compare responses of patients toreal medication vs. placebo

    Compare attention spans ofundergraduate students in differentprograms at PC.

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    Why Not Just Use t-

    tests? Tedious when many groups are

    present

    Using all data increasesstability

    Large number ofcomparisons some may

    appear significant by chance

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    Remember that Standard deviation (s)

    n

    s = [( (xi X)2)/(n-1)]i= 1

    In this case: Degrees of freedom (df)

    df = Number of observations or groups - 1

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    Notation

    k= # of groups n= # observations in each group xij= one observation in group i

    Y= mean over all groups Yi= mean for group i SS = Sum of Squares MS = Mean of Squares = Between MS/Within MS

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    FYI this is how SS

    Values are calculatedk ni Total SS = (xij )2 = SStot

    i=1 j=1

    k ni

    Within SS = (xij i)2 = SSwi=1 j=1

    k ni

    Between SS = ( i )2 = SSbeti=1 j=1

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    and SStot = SSw + SSbet

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    Calculating MS Values MS = SS/df

    For between groups, df = k-1 For within groups, df= n-k

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    Hypothesis Testing &

    Significance Levels

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    F-Ratio = MSBet/MSw If:

    The ratio of Between-Groups MS:

    Within-Groups MS is LARGE rejectH0 there isa difference betweengroups

    The ratio of Between-Groups MS:Within-Groups MS is SMALL do notreject H0 there is nodifferencebetween groups

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    p-values Use table in stats book to determine

    p

    Use df for numerator anddenominator

    Choose level of significance

    If F > critical value, reject the null

    hypothesis (for one-tail test)

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    Example 1, pp. 400 of

    your handout Three groups:

    Middle class sample Persons on welfare

    Lower-middle class sample

    Question: Are attitudes towardwelfare payments the same?

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    So,

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    and

    From the table with = 0.05 and df = 2 and 24, we see thatif F > 3.40 we can reject Ho. This is what we would

    conclude in this case.

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    Example 2 Bat cave gates:

    Group 1 = No gate (NG)

    Group 2 = Straight entrance gate (SE) Group 3 = Angled entrance gate (AE) Group 4 = Straight dark zone gate (SD) Group 5 = Angled dark zone gate (AD)

    Question: Is variation in bat flightspeed greater within or betweengroups? Or Ho = no differencesignificant difference in means.

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    Just leave me alone

    Max! Go back to

    your hockey!

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    Example 2 (contd)Group #,

    i

    Gate

    Type

    Mean FS (m/s) sd FS (m/s) ni

    1 NG 5.6 0.93 150

    2 SE 3.8 1.05 150

    3 AE 4.7 0.97 150

    4 SD 4.2 1.23 137

    5 AD 5.1 1.03 143

    Hypothetical data for bat flight speed with various gate arrangements.

    FS= Flight speed; sd = standard deviation

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    Example 2 SSbetBetween SS= 300

    Group

    #, i

    Gate

    Type

    Mean FS

    (m/s)

    sd FS (m/s) ni

    1 NG 5.6 0.93 150

    2 SE 3.8 1.05 150

    3 AE 4.7 0.97 150

    4 SD 4.2 1.23 137

    5 AD 5.1 1.03 143

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    Example 2 SSw

    Within SS = 790

    Group

    #, i

    Gate

    Type

    Mean FS

    (m/s)

    sd FS (m/s) ni

    1 NG 5.6 0.93 150

    2 SE 3.8 1.05 150

    3 AE 4.7 0.97 150

    4 SD 4.2 1.23 137

    5 AD 5.1 1.03 143

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    Example 2 (contd) Between MS = 300/4 = 75

    Within MS = 790/(730-5) = 1.09

    F Ratio = 75/1.09 = 68.8

    See Table find p-value based ondf= 4,

    Since F>value found on the table we

    reject Ho.

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    What ANOVA Cannot

    Do Tell which groups are different

    Post-hoc test of mean differencesrequired

    Compare multiple parametersfor multiple groups (so it cannot

    be used for multiple responsevariables)

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    Some Variations Two-Way, Three-Way, etc.

    ANOVA (will talk about this nextclass)

    2+ factors

    MANOVA (Multiple analysis ofvariance)

    multiple response variables

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    Summary ANOVA:

    allows us to know if variability in a data

    set is between groups or merely withingroups

    is more versatile than t-test can compare multiple groups at once

    cannot process multiple responsevariables does not indicate which groups are

    different

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    Now, lets go to our

    SPSS manual Perform the sample problem on the effects

    of attachment styles on the psychology ofsleep with the data set from the NAAGEsite called Delta Sleep.

    Pay attention to the procedure and thepost-hoc tests to determine which groupsare significantly different. Perform the

    Tukey Test at a 5% significance level. Look at your output and interpret your

    results.

    Tell me when you are done.

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    So, you had

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    Then, following the steps

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    You got

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    and

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    What do all these

    mean?

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    When you are done

    with this, Do practice exercises 1, 4, 6, 7

    and 12 from the handout inSPSS.

    Create the data sets.

    Run the one-way ANOVAS andinterpret your results.