9 pt propagation - vetiver.org workshop ppt/kuw-ppt10.pdf · proliferation stage (modified 2 mg...
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VETIVER SYSTEM
Propagation and Nursery
Dr. Paul TruongVeticon Consulting
Brisbane , Australiawww.uqconnect.net/veticon
All materials in this document remain the property of Veticon Consulting P/L.Permission must be obtained for their use. Copyright © 2002
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Sexual propagation: by seeds?
Florescences of Vetiver 3, 5, 10, 15 days after floweringhaving about 2,000 flowers each
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Flower structureSessile spikelet onlycontains stigma withoutanther
Pedicelled spikeletcontains stigma andanther
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Pollen Development
Anther of flowers 7days after flowering Separated anther in
vitro
Pollen grain invitro
Pollen grain not germinating13 days after sowing in vitro
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Germination of seeds?
Inflorescence 20-25days after flowering
Seeds were sowed invitro
Seeds do not germinate14 days after sowing
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Sexual propagation: by seeds?Conclusion
From the investigations, ecotype (V. zizanioides)
• Vetiver induces flowers
• Seeds do not have endosperm, thereforeno germination
• This ecotype can not become a weed
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The two commonly used methods for large scalepropagation of vetiver are:
1- Separation or splitting* a mature vetiver plant.* side shoots* culm cuttings
2- Tissue culture
Asexual Propagation Methods
Goals: High multiplication rate and low cost price
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Asexual propagation by splittingfrom a mother plant
Shoot and root growth fromthe slips
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Bare root slips: Dipping in rooting hormone (optional)
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Bare root slips after about 10-15 days in hormone
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Bare root slips ready for planting directly to site or to polybags
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Bare root slipstransplanted to poly
bags
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Polybags ready for planting directly to site
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Bare root slips on the left and tube stocks on the right
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A mature culm caninitiate side shoot undercertain conditions
Asexual propagation from a mother plant
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Asexual propagation by culms
Matureshoots
Youngshoots
Results:with 85-90%rootedwithouthormonaltreatment
Culm Cuttings
Results:with 65%rootedwithouthormonaltreatment
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Culm cutting
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Root growth from bare root slips and culm cuttings
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Planting strip: For immediate results and fast planting
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Aerial root pruning: To limit root growth to reduce shock afterplanting and for ease of transport
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Lateral buds
In vitro
Induce explants
+ CytokinineProliferationin thenethouseconditions
AcclimatizationMultiplication in fieldPlantlets ready
Asexual propagation by tissue culture
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High multiplication rate (hypothetically 58million plantlets per year)
Proliferation stage (modified 2 mg BA/liter)carrying out in the shade house will bereduce 13% production cost of eachplantlets
This method was developed at CanthoUniversity, Vietnam and has been usedsuccessfully in the last few years forcommercial production
Asexual propagation by tissue culture
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Advantages and Disadvantage ofBare Root Slips
Advantages:• Very efficient, low cost and fast to prepare the planting material• Small volume for transport, ie lower delivery cost• Very easy to plant out by hand• Can be mechanically planted out for large areas
Disadvantages:• Vulnerable to dryness and extreme temperature• Limited storage time on site• Need to be planted to moist soil• Need more frequent irrigation in the first few weeks.• Recommended for good seedbed sites with easy access toirrigation
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Advantages and Disadvantage ofpolybags or tube stock
Advantages:• Very hardy and is not affected to exposure to high temperaturean moisture stress• Lower irrigation frequency after planted out• Faster establishment and growth after planted out• Longer period of storage time on site• Recommended for harsh and hostile environments
Disadvantages:• More costly to produce• Longer period to prepare, 4 -5 weeks or more• Large volume and heavy load for transport, ie higher deliverycost• More maintenance cost at site after delivery, if nor planted outwithin a week
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Advantages and Disadvantage ofTissue Culture Method
Advantages:• A very large number of plant can be produced very quickly• No need for a large scale nursery• Smaller volume and weight for transportation• Free from pest and pathogen in nursery
Disadvantages:• The need to set up a small laboratory, which can be expensive for asmall nursery• The need for a well trained technician and other skilled staff• The need for more manual labour to transfer the seedling todifferent size pots during its growing period.• It takes longer to get the plantlets ready for planting• More susceptible to pest and disease on site and adverse conditions
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NurseryThe main points in establishing an efficient nursery are:
Soil type: For the ease of harvesting and minimizingroot and crown damages, sandy loam to loam is recommended
Planting method: Mechanical or manualHarvesting method: Harvesting the mature plants can
be done either mechanically or manually. For machineharvesting
Availability of farm machinery: Some basic farmmachineries are needed for seed bed preparation, weedcontrol, cutting, harvesting etc.
Irrigation method: Overhead irrigation or floodirrigation
Training of operational staff: Availability of welltrained staff is essential to the success of the nursery
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Manual planting in Australia, overhead irrigation andharvested by potato digger
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Machine planting on flat ground, overhead irrigation and harvestedby mouldboard plough
Machine planting in Australia,
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A vegetable planter was modified for planting vetiver bare root slips
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Either single or double line
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On site nursery in Vietnam
For large project, temporary nursery can beestablished close to the project site
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Planting sites
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Large scale nursery in China
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Vetiver nursery in Guangdong, China. Mature plants background,new planting foreground
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Thank You