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A new chapter in GC-MS Thermo Scientific Q Exactive GC Orbitrap GC-MS/MS System

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Page 1: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

A new chapter

in GC-MS

Thermo Scientific Q Exactive GCOrbitrap GC-MS/MS System

Page 2: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

2

Unprecedented Depth in GC-MS Analysis

“The introduction of the Q Exactive GC

system is a game changer in this space.”

Prof. Joshua Coon

Department of Chemistry

University of Wisconsin, USA

Proteomics and Metabolomics

“This is clearly a major step forward.”

Dr. Hans Mol

RIKILT, Netherlands

Natural Toxins and Pesticides

“GC Orbitrap technology will bring a new level of performance to high resolution GC-MS.”

Prof. Jana Hajšlová

University of Chemical Technology

Prague, Czech Republic

Food Safety and Food Authenticity

“This is the technology platform I have been waiting for since I started in metabolomics.”

Dr. Karl Burgess

Glasgow Polyomics

University of Glasgow, UK

Metabolomics

Page 3: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

3

“GC Orbitrap technology significantly expands the firepower of GC-MS, which will spark an acceleration in discovery for small molecule analysis across multiple applications.”

Dr. Alexander Makarov

Director of Research

Life Science Mass Spectrometry

Thermo Fisher Scientific

The Thermo Scientific™ Q Exactive™ GC Orbitrap GC-MS/MS system has finally arrived. For years,

scientists have demanded this highly anticipated, high-resolution, accurate-mass (HR/AM) system in

fields ranging from metabolomics to food safety, industrial, clinical, and pharmaceutical analysis. These

scientists understand the power that only Thermo Scientific™ Orbitrap™ technology can bring to the

detailed characterization of their most challenging samples.

Unprecedented Depth in GC-MS Analysis

Until now, GC-MS technology has been limited in its ability to collect

comprehensive qualitative and quantitative sample information with

high levels of selectivity, sensitivity and confidence; especially in highly

complex samples.

Today, the Q Exactive GC system makes this comprehensive analysis

a reality. It is an easy-to-use, dedicated benchtop GC-MS system that

provides the highest confidence in compound discovery, identification,

and quantitation for a comprehensive understanding of your samples.

This unmatched performance is achieved through the superior

resolving power, mass accuracy, and sensitivity that only Orbitrap

technology can deliver.

The capabilities of the Q Exactive GC system create a paradigm shift

for analysis of GC-amenable compounds and signal the start of an

exciting new chapter in GC-MS…

Page 4: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

d i s c o v e r

4

the ultimate profiling toolSuperior Compound Discovery and Identification

The Q Exactive Orbitrap GC-MS/MS system provides you with the power to discover features within a sample that went unnoticed with

previous GC-MS technology. This unrivalled combination of high-resolution gas chromatography separations and highly sensitive HR/AM

acquisitions and intelligent identification software will help unlock answers to questions about the very nature of each sample you inject.

High capacity component detectionPart-per-trillion level sensitivity of the Q Exactive GC system in full scan with a wide dynamic range and HR/AM drives high capacity component detection, even in your most complex samples.

Highly resolved components for chemometric analysis and compound identificationIn addition to the highest quality raw data, the Q Exactive GC system uses newly developed specialist deconvolution software tools to automatically detect components within your sample and generate clean spectra for further data processing steps.

Principal component analysis (PCA) generated using Thermo Scientific™

SIEVE™ software during a study of the metabolic consequences of

perturbation to the pentose phosphate pathway in Leishmania mexicana

using the Q Exactive GC system: labelled glucose tracking metabolism in wild

type (WT) and transgenic mutant (TKT).

The Q Exactive GC brings highly resolved data that allows for

advanced deconvolution providing high quality inputs for downstream

contextualization software. – Karl Burgess

50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210m/z

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180.00957

169.93473

73.01072 115.05767

63.94372142.99848

127.88781

95.91574

50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210m/z

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100 69.06994

129.03696

135.94709178.0418158.99523 146.0219285.01073 212.00284197.98722116.9828799.02645

153.03693

50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210m/z

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100 73.01070

105.99064

180.00956

169.93470

149.04536

115.05767

63.94372142.99847131.05261

78.96710

105.99065

Co-eluting compounds are deconvoluted to obtain clean mass spectra

Full scan acquisition (60,000 resolution at m/z 200) of a food packaging sample from an

extractables and leachables study. A single, unidentified component is visible within the complex

matrix background.

Page 5: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

i d e n t i f y

5

The ability to predict elemental formulae of each EI fragment is hugely powerful for metabolite identification.

– Karl Burgess

Powerful software for intelligent identificationThe Q Exactive GC system uses vast, readily available nominal mass electron ionization (EI) libraries (such as NIST and Wiley) to generate candidate compounds. Hits are verified using intelligent exact mass interpretation of the fragmented mass spectrum acquired. Customized HR/AM libraries can also be used

This technology will greatly facilitate the identification of unknown compounds.

– Joshua Coon

High confidence confirmationFor further information and/or confirmations of compounds, positive chemical ionization (PCI) can be used to yield molecular ions. These ions can be measured directly in the Orbitrap or after fragmentation in the HCD for further HR/AM investigation into the compound structure.

EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment

structure assignment using Thermo Scientific™ Mass Frontier™ spectral interpretation software.

High quality deconvolution of spectra from a Scotch whiskey sample during an authenticity study.

Automatic identification of unknown peaks was performed using intelligent identification software.

EI

CI MS/MS

0.56 ppm

0.58 ppm

0.14 ppm

0.07 ppm

0.18 ppm 0.44 ppm

0.25 ppm

CI

50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 0

20

40

60

80

100214.08533

173.04620

141.00049 216.08235

77.03861 170.02699 175.04320 104.00104 132.03233 158.02283 68.02437 145.01498 96.05561 110.04614 83.06037 119.03710 178.10860 160.01980

50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 0

20

40

60

80

100

Rel

ativ

e A

bund

ance

138.07743

174.05407

176.05108 214.08528 194.14000 202.08529

216.08239 156.02425

50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 m/z

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172.03835 C 5 H 7 N 5 Cl 138.07741

C 5 H 8 N 5

110.04614 C 3 H 4 N 5 68.02435

C 2 H 2 N 3

145.01495 C 3 H 4 N 5 Cl

136.06178 C 5 H 6 N 5

79.00574 C H 4 N 2 Cl

104.00099 C 2 H 3 N 3 Cl

94.03996 C 4 H 4 N 3

214.08527 C 8 H 13 N 5 Cl

158.02276 C 4 H 5 N 5 Cl

71.06038 C 3 H 7 N 2 61.97932

C H N Cl

N

N

NH2+

N

N

NH

NH

NH2+

+NH2

NN

N

NH

NCl

N

NH

NH

+NH2 NCl

N

NH

N

NH2

+

NCl

N

NH

N

NH2

+

NCl

N

NH

N

+NH2

Analyzing whiskey and pesticide samples, to my

surprise, many compounds where automatically identified.

– Jana Hajšlová

Page 6: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

d e t e c t

the ultimate screening toolPowerful, broad-scope sample screening and quantitation

Analyzing compounds present at low concentrations, quantitatively, with high selectivity using full scan acquisition has been out of reach for

GC-MS users for too long. Many leading laboratories in various fields require simultaneous targeted and untargeted sample screening, but

that need went unmet until now. Today, the Q Exactive GC system has the quantitative power of a GC triple quadrupole MS combined with

the high precision, full scan high resolution/accurate mass capability that only Orbitrap technology can offer. This combination will change

your workflows, forever.

Confident low-level detection and identification for high efficiency screening workflowsThe Q Exactive GC system offers routine operation, possible at 60k–120k resolution (at m/z 200) with sub-1 ppm mass accuracy and full scan sensitivity down to part-per-trillion concentrations. This capability drives truly powerful screening methods for food safety, environmental, anti-doping, and many other applications where ultra trace-level detection is required in difficult matrices.

For pesticides, I could safely see very low levels.

– Jana Hajšlová

6

Mass accuracy across n=14 repeat injections of a baby food sample spiked

with several pesticides at 5 ng/g.

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

1

2

3

4

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13

14 Bifenthrin

Chlorpyrifos-ethyl

Chlorthal-dimethyl

Clomazone

2-phenylphenol

Acetochlor

Benalaxyl

BHC, Alpha

Methacrifos

Metribuzin

Nitrothal-isopropyl

Oxadiazon

Pentanochlor

Permethrin

Phosalone

Pirimiphos-ethyl

Propanil

Quinomethionate

Tecnazene

1-14 injection number

120k 60k 30k 15k chlorpropham 0.0 0.5 0.9 18.4 Procymidone 0.5 0.0 0.7 8.6 Iprodione 0.2 0.4 3.1 4.9

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

20.0

mas

s di

ffere

nce

[ppm

]

Effect of resolution (specified @ m/z 200) on mass accuracy for pesticides in leek

samples at 10 ng/g (above). Acquisition at 15k yield false negative results based

upon identification criteria. Chlorphropham example XIC, below, shows resolution

values of 60k and 120k, providing interference-free, confident detection with

excellent mass accuracy.

i d e n t i f y

30K

60K

120K Chlorpropham Matrix

Mass difference = 18.4 ppm

Mass difference = 0.9 ppm

Mass difference = 0.5 ppm

15K

Mass difference = 0 ppm

127.008 127.010 127.012 127.014 127.016 127.018 127.020 127.022 127.024 127.026 127.028 127.030 127.032 127.034 m/z

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e

127.02067

127.008 127.010 127.012 127.014 127.016 127.018 127.020 127.022 127.024 127.026 127.028 127.030 127.032 127.034 m/z

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127.02164 127.01821

127.008 127.010 127.012 127.014 127.016 127.018 127.020 127.022 127.024 127.026 127.028 127.030 127.032 127.034 m/z

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127.02117 127.01826

127.008 127.010 127.012 127.014 127.016 127.018 127.020 127.022 127.024 127.026 127.028 127.030 127.032 127.034 m/z

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e 127.01833

127.02118

127.02261

Page 7: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

The wide dynamic range of the system, along with its high sensitivity, will bolster all quantitative experiments dramatically.– Joshua Coon

Quantitation capability beyond that of other benchtop HR/AM GC-MS systemsUsing full scan acquisition, the Q Exactive GC system provides the quantitative capability of a GC triple quadrupole instrument operating in selected reaction monitoring (SRM) mode. Sensitivity,quantitative accuracy, precision, and linearity are excellent, even in complex matrices.

7

This technology provides an easier, more efficient, and more comprehensive way of

doing GC-based pesticide residue analysis.– Hans Mol

Thermo Scientific™ TraceFinder™ software uses Q Exactive GC system data and combines compound

detection, identification, and quantitation to enable high efficiency screening. It also allows

customizable, automated flagging for detected peaks along with highly flexible reporting tools.

Wheat matrix calibration curve of chlorpropham (0.5 ppb–50 ppb) including extracted quantifier and

qualifier ions at 0.5 ppb. Acquired using 60k resolution at m/z 200.

q u a n t i f y

Instrument detection limit (IDL) of 150 pesticides in mixed vegetable matrix using the Q Exactive GC

system (full scan 60k at m/z 200) and Thermo Scientific TSQ™ 8000 Evo GC-MS/MS system (SRM) with

a fast GC separation.

Q Exactive GC System IDL ng/g <0.5

0.5-1.0

1.0-2.0

2.0-4.0

TSQ 8000 Evo GC-MS/MS IDL ng/g <0.5

0.5-1.0

1.0-2.0

2.0-4.047%

22%

22%

9%

49%

16%

33%

2%

Page 8: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

8

Hexachlorobenzene ions

m/z 283.80962 m/z 248.84076 m/z 176.90601 m/z 141.93716Concentration ng/ml mass error (ppm) mass error (ppm) mass error (ppm) mass error (ppm)

0.2 0.5 0.2 0.2 0.2

1 0.8 0.4 0.2 0.3

2.5 0.7 0.4 0.0 0.3

10 0.8 0.7 0.1 0.4

50 0.8 0.8 0.1 0.5

1000 1.0 1.0 0.3 0.7

5000 0.8 0.8 0.2 0.5

250000 0.8 0.5 0.0 0.4

Average mass error (ppm) 0.8 0.6 0.1 0.4

ORBITRAP MASS ANALYZER

Incredible resolving power up to 120,000 FWHM at m/z 200, sub-ppm mass accuracy with a high acquisition rate, driving

unrivaled spectral quality

C-TRAPCurved linear trap for precise

ion injection—delivers excellent in-spectrum dynamic range and

ensures outstanding HR/AM performance across a wide range of concentrations through automatic

gain control (AGC)

HCD COLLISION CELLHigher-energy collisional dissociation for MS/MS

characterization of ions—used in combination with chemical ionization to help elucidate

chemical structures

ADVANCED QUADRUPOLE

TECHNOLOGY (AQT)High transmission of selected masses for low-level detection

and quantitation of low- abundance compounds

in highly complex matrices

150 160 170 180 190 200 210 220 230 240 250 260 270m/z

0

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235.00762R=118356C13 H9 Cl2

165.06989R=140457C13

H 9

199.03090R=126265C13 H8 Cl176.06205

R=134582C

14H8

212.03871R=119900C14 H9 Cl

239.00168R=120013C

13 H937Cl2

0.2 ppm

0.06 ppm

0 ppm0 ppm

0.06 ppm

p,p '-DDTC

14H

9Cl

5

bringing GC and Orbitrap technology togetherHigh performance, high productivity design

Time (min)

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1007.69

262.85651

7.675 7.680 7.685 7.690 7.695 7.700 7.705 7.7100

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7.69262.85651

7.69262.85654

7.69262.85651

7.69262.85657

7.70262.85651

7.69262.85651

7.70262.85654

7.68262.85654

7.70262.85657

7.68262.85657 7.70

262.85657

7.68262.85660

7.71262.856607.68

262.85660 7.71262.85654

0.7

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R = 59097

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Page 9: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

The operation and software handling is very simple. – Jana Hajšlová

9

BENT FLATAPOLE

For lowest possible noise and maximum

robustness

Thermo Scientific™ ExtractaBrite™ ION SOURCE

Robust, rugged electron impact (EI) and chemical ionization (CI) performance – proven

in routine applications in the Thermo Scientific™ ISQ™ and TSQ 8000 series GC-MS systems. Fully removable without breaking vacuum for

maintenance or switching to chemical ionization (CI). Also exchangeable for a unique source plug, making GC columns easily exchanged,

without venting the MS system

Fastest route to powerful dataWith the Q Exactive GC system it is easy to obtain powerful results. The instrument control page provides clear instrument status information, leak check is automated, and mass spectrometer tuning and calibration takes less than a minute.

ORBITRAP MASS ANALYZER

Incredible resolving power up to 120,000 FWHM at m/z 200, sub-ppm mass accuracy with a high acquisition rate, driving

unrivaled spectral quality

bringing GC and Orbitrap technology togetherHigh performance, high productivity design

Time (min)

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262.85651

7.675 7.680 7.685 7.690 7.695 7.700 7.705 7.7100

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7.69262.85651

7.69262.85654

7.69262.85651

7.69262.85657

7.70262.85651

7.69262.85651

7.70262.85654

7.68262.85654

7.70262.85657

7.68262.85657 7.70

262.85657

7.68262.85660

7.71262.856607.68

262.85660 7.71262.85654

0.7

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R = 59097

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Page 10: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

10

Tailor the Thermo Scientific™ TRACE™ 1310 Gas Chromatograph to

your needs with its proprietary user-exchangeable Instant Connect

injector and detector modules. Swapping modules is easy to do

by removing and replacing three screws, accessible from the top

of the GC. The entire process takes less than five minutes without

requiring specialized service assistance. This modularity enables

budget-conscious laboratories to purchase a base-configured GC

today, then easily expand their capabilities to accommodate new

applications and throughput demands tomorrow.

Instant Connect In jectorsInstant Connect SSLThe Instant Connect SSL (Split/Splitless) injector features an optimized thermal profile developed to avoid sample discrimination in split and splitless mode, allowing the broadest range of analytes to be accurately injected. Its cool injector head guarantees minimum thermal stress to the septum, therefore reducing bleed and extending septa lifetime. Also available with concurrent backflushing.

Instant Connect—PTVThe Instant Connect PTV (Programmed Temperature Vaporizing) injector combines the “discrimination-free” performance of a cold injector with the robustness of a vaporizing injector. Merging fast heating and cooling performance with the inertness of the injector chamber and large sample capacity, this injector is the ideal choice for trace analysis in dirty matrices and for thermally labile compounds. Also available with concurrent backflushing.

Instant Connect Helium Saver ModuleA unique solution to a difficult problem, this proprietary split/splitless injector module greatly reduces helium consumption, by using helium only to supply carrier gas flow to the capillary column, while using nitrogen for all other injection processes, including the septum purge, split, and sample vaporization.

Instant Connect DetectorsInstant Connect-FIDThe Instant Connect-FID (Flame Ionization Detector) offers the highest sensitivity and a wide dynamic range with rapid acquisition speed, making it ideal for extremely fast GC applications.

Instant Connect-TCDThe newly-designed micro-volume Instant Connect-TCD (Thermal Conductivity Detector) is used in a wide variety of capillary and packed column applications. Due to its exceptional thermal stability and fast response, this non-destructive detector provides exceptional sensitivity over the widest range of applications.

Instant Connect-ECDThe new Instant Connect-ECD (Electron Capture Detector) is excellently optimized for trace analysis in challenging samples. Its miniaturized cell, equipped with a purged, removable anode, has been designed to guarantee the utmost sensitivity while maximizing robustness towards the matrix effect.

Instant Connect-NPDBuilt upon the proven sensitivity of the Thermo Scientific Nitrogen Phosphorous Detectors (NPD), the new Instant Connect-NPD brings exceptional flexibility to the determination of specific components with the adoption of multiple dedicated ion sources.

Instant Connect-FPD The Instant Connect-FPD (Flame Photometric Detector) provides excellent sensitivity and response stability for the most demanding sulphur applications, as well as phosporous or tin determinations. Its extended operating temperature range and dual wavelength capability further expand its applicability.

Transcend the traditionalstep into modern gas chromatography

Page 11: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

11

A step ahead in automated sampl ingA perfect match for the Q Exactive GC system, the Thermo Scientific™ TriPlus™ RSH autosampler utilizes robotic sample handling to expand automated capabilities beyond liquid, headspace, and solid-phase microextraction (SPME) injections to advanced sample handling cycles. Your results benefit from improved precision and reproducibility, while your laboratory gains unique advantages from the system’s unattended operations and sample handling flexibility.

• Automate basic sample and standards preparation procedures such as dilution, internal standard addition, and derivatization.

• High precison injection from low volumes to maximize your analysis opportunities with your most precious samples

• Automated toll exchange to switch between different syringes and techniques “on-the-fly”

Fast oven temperature cycling and excellent retention time performance mean that high-precison, high-productivity data sets can be realized. This type of data is especially important to facilitate statistics in larger batches, e.g., in “-omics”-type studies with many biological or technical replicates.

The TRACE 1300 Series GC quickly reaches near-ambient temperatures.

Warm-up Times. From OFF Conditions to Readiness (minutes)

Oven at 50 °C

Injector and Detectors at 250 °C

TRACE 1300 Series GC Standard GC

3.5 10.2

Outstanding retention time stability is achieved, even in the most complex GC and GC-MS applications, through the use of innovative and unique IEC (integrated electronic control) modules. This stability guarantees industry-leading 0.001 psi control through the entire working range. These miniaturized gas controls, integrated within every injector or detector module for compact, self-sufficient fully-featured devices, deliver strictly controlled pressure or flow to columns and detectors.Setting constant or ramped pressures and flows is easy through the software or the local user interface, while the electronic control maintains the stability during every run for exceptional retention time, accuracy, and precision. To further enhance analytical performance, the IEC module also supports the automated leak check of the injector and column installed and column evaluation procedures.

Retention time stability on 10 consecutive runs of hydrocarbon mix. Retention time standard

deviation is always ≤1/1000 minute.

Except ional Retent ion Time Stabi l i ty

Hydrocarbon Mean RT Min. Std. Dev. Min. Hydrocarbon Mean RT Min. Std. Dev. Min.

C12 4.6200 0.0003 C28 12.4725 0.0005

C14 6.0192 0.0004 C30 13.1348 0.0006

C16 7.2268 0.0005 C32 13.7557 0.0006

C18 8.3051 0.0005 C34 14.3395 0.0007

C20 9.2825 0.0006 C36 14.8908 0.0005

C22 10.1767 0.0006 C38 15.4118 0.0007

C24 10.9997 0.0004 C40 15.9063 0.0006

C26 11.7629 0.0005

1 µL splitless injection

40 ppm C20 in toluene

Volume in vial (μL) Peak area

50 81244277

40 80268993

30 82088809

20 82095395

10 84436788

5 84312030

RSD% 2.0

The TriPlus RSH autosampler provides excellent repeatability with

microsamples, down to 5 µL in a vial. This feature is particularly

interesting for trace analysis, radioactive samples, or samples

requiring expensive internal standards.

step into modern gas chromatography

Page 12: A new chapter in GC-MS · EI, PCI, and PCI – MS/MS spectra (60,000 resolution at m/z 200) of terbuthylazine with fragment structure assignment using Thermo Scientific ™ Mass Frontier

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THERMO SCIENTIFIC CHROMATOGRAPHY

COLUMNS AND CONSUMABLES

Get the most out of the Q Exactive GC system by pairing it with advanced, high-performance Thermo Scientific products. Our wide range of consumables and accessories offer customers applications-focused solutions in the environmental, food safety, toxicology, clinical, petrochemical, pharmaceutical and general analytical industries.

• Thermo Scientific™ TraceGOLD™ columns – low bleed, high reproducibility • Consumables tested and certified on the Thermo Scientific TRACE 1300 Series GC • Vials guaranteed for Thermo Scientific autosampler systems• GFM Pro Gas Flowmeter and GLD Pro Gas Leak Detector for system installation and maintenance• Derivatization reagents and Derivatization grade solvents

Learn more: thermoscientific.com/ChromExpert