a novel, natural anti-microbial product for use as an ... · a novel, natural anti-microbial...
TRANSCRIPT
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Willem Ravensberg R&D Microbials
Koppert Biological Systems
Berkel & Rodenrijs, the Netherlands
A novel, natural anti-microbial product for use as an agricultural
bactericide and fungicide
1st Annual Biocontrol Industry Meeting, October 23-24, 2006, Lucerne, Switzerland
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Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicide LP as a bactericide LP against virusesCompatibility Conclusions
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What is the Lactoperoxidase system?
Occurs in animals and humansImportant non-immune defence systemActive system in milk, saliva, tears, etc.Used in food products and oral care productsKoppert and DMV/Campina have developed it as a natural fungicide/bactericide
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Natural system based on:- 1 enzyme + 2 substrate components:
lactoperoxidase, SCN- and H2O2
Based on the formation of reactive oxygen species that inhibit or kill bacteria FAO: milk preservation: addition of SCN- and H2O2
What is the LP system?
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LP in oral care products
Toothpaste (Zendium, Biotene)
Chewing gum (Biotene, Bio-Xtra)
Mouth rinses (Oralbalance, Biotene)
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Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicide LP as a bactericide LP against virusesCompatibility Conclusions
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Koppert s LP system?
System based on:- 2 enzymes + 3 substrate components - adjuvant based on a vegetable oil
Based on the formation of reactive oxygen species that inactivate proteins in micro-organisms
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Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicide LP as a bactericide LP against virusesCompatibility Conclusions
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Mode of Action LP-system
H2O
OSCN-
OI- HS-R
HOS-RSCN-
I-
LP
H2O2
LACTOPEROXIDASE SYSTEM:
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Mode of action
Oxidation of sulfhydryl groups in proteinsMembrane effectsActive transports of amino acids and sugars disturbedGlycolytic enzymes inhibitedOther metabolic functions disturbed
cell death
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Activity (1)
Activity based on contactCurative actionActivity not translaminar nor systemic No preventive effectNo residual effectOnly active in water phase High Volume spray not dependent on temperature and relative humidity
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Activity (2)
Activity on - fungi - yeast- bacteria- viruses - mycoplasma s
Kills spores, cells, myceliumNo resistance development possible due to multiple-site activity
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Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicideLP as a bactericide LP against virusesCompatibility Conclusions
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Research
Target 1: powdery mildew(s)
Protected crops worldwide
Research model: cucumber/Sphaerotheca
Composition of active system
Formulation, incl adjuvant
Product developed
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KBV 99-01 in cucumber
Control of Powdery Mildew (Sphaerotheca fuliginea ) in cucumber (M. Post, July 2003, Nl)
0
10
20
30
40
50
60
70
80
90
100
Baycor flow KBV 99-01 (1N) KBV 99-01 (2N)
effic
acy
% o
f co
ntr
ol
2 days after treatment 8 days after treatment
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Visible effect in cucumber
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KBV 99-01 in tomato
Control of Powdery Mildew (Oidium lycopersicum ) in tomato (2002 and 2003, Nl)
0
10
20
30
40
50
60
70
80
90
100
grower 1(Dec '02)
grower 2(May '03)
grower 3(Aug '03)
effic
acy
% o
f co
ntr
ol
Baycor flow KBV 99-01 (1N) KBV 99-01 (2N)
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Visible effect in tomato
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Research
Target 2: Fusarium oxysporum
Bulbs
Research model: tulip/ zuur
Other bulbs and diseases in research
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Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicide LP as a bactericideLP against virusesCompatibility Conclusions
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Research
Target: bacterial diseases
Lab trials on Erwinia, Clavibacter, Pseudomonas, Xanthomonas spp. positive
Further research needed
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Research
Apple: fire blight
Lab trials positive
Field trial: result moderate
Further research: formulation and application strategy
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Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicide LP as a bactericide LP against virusesCompatibility Conclusions
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LP against viruses
Medical uses reported
In vitro trials - TMV - PepMV
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LP against viruses
Results:
TMV no effect
PepMV variable results up to 95% effect
More research needed
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Patent
Composition and application patented
For use as an agricultural fungicide / bactericide
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Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicide LP as a bactericide LP against virusesCompatibility Conclusions
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360DirectNymphs (L1)Orius laevigatius
00
0
0
015
20
56
97
23
22
22
1714
15
Corrected mortality (%)
11
OralContact
WorkersApis mellifera
1ResidueReproductionreduction
1ResidueNymphs (L2-3)
11
ResidueDirect
Nymphs (L2)Macrolophus caliginosus
1ResidueEretmocerus mundus
3ResidueEncarsia formosa
11
ResidueDirectMummies
Aphidius colemani
1ResidueReproductionreduction
1ResidueProtonymphsTyphlodromus pyri
1ResiduePhytoseiulus persimilis
11
ResidueDirectEggs
Amblyseius swirskii
1ResidueAmblyseius cucumeris
IOBCcategory
Direct / residue
Life stageNatural enemy
Compatibility Enzicur with natural enemies
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SulphurThiophanate-methyl
Triflumizole
Bicarbonate
Imazalit
Chloorthalovit + prochloraz
Captan
Bitertanol
Azoxystrobin
Fungicides
ThiovitTopsin-M
Mycotal Verticillium lecanii
CalypsoThiacloprid
NomoetTeflubenzaron
OberonSpiromesfen
AdmiralPyriproxifen
RocketAsepta CarexPyridaben
MilstopPlenemPymetrozin
FungaflorPirimozPirimicarb
AllureAdmire Imidacloprid
CaptanTrigardCyromazin
BaycorDipelBacillus thuringiensis
OrtivaVertimecAbamectine
Commercial name
Commercial name
Insecticides
Compatibility Enzicur with pesticides
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Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicide LP as a bactericide LP against virusesCompatibility Conclusions
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Advantages of LP system
A unique natural productNo resistance Safe to humans, plants, animalsand the environmentSafe to natural enemies and bumblebeesFits in Integrated Pest Management Fits in organic agriculture
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Conclusions
A new type of fungicide and bactericide
New mode of action
Contact activity
Easy to use
Potentially for a broad range of diseases and applications
Contact: [email protected]
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Koppert Biological SystemsKoppert takes you further, naturally
Thank you for your attention!
www.koppert.com