A Symposium

CONNECTING EXCELLENCE: University of Toronto-Karolinska Institutet

May 26th, 2015

Peter Gilgan Centre for Research and LearningHospital for Sick Children

Toronto, Canada

Welcome

UofT Coordinators:Stephen Matthews Janet Rossant

KI Coordinators:Ola Hermanson Klas Blomgren

CONNECTING EXCELLENCE: University of Toronto-Karolinska Institutet

Sponsors

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Welcome to the International Symposium: Connecting Excellence. The objective of this Symposium is to highlight the outstanding research that is being undertaken at the University of Toronto and the Karolinska Institutet in the fields of; Developmental Health, Stem Cells and Regenerative Medicine, and Transplantation and Tissue Engineering. By highlighting research in these areas, we hope to begin to develop new and innovative collaborations. This would build on a long–standing (~20 year) formal relationship between our Institutions, which has included an annual exchange program in ‘Developmental and Perinatal Biology’ and most recently the Mats Sundin Postdoctoral Fellowship Program.

We would like to acknowledge the support of the University of Toronto and the Karolinska Institutet, as well as that of the other sponsors of this event. We hope that you enjoy the day!

Program

Wednesday | May 26th, 2015

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7:30 am BREAKFAST8:00 am WELCOME

Trevor Young, Dean of Medicine, University of TorontoVivek Goel, Vice-President Research, University of Toronto

8:20 am DEVELOPMENTAL HEALTHChairs: Stephen Matthews (UofT) & Ola Hermanson (KI)Stephen Matthews (UofT): Why the 1st 2000 days of life really matter!Rickard Sandberg (KI): Analyses of gene expression in single cellsJanet Rossant (UofT): Establishing pluripotency in mouse and human developmentKlas Blomgren (KI): The Phoenix effect - Dying stem cells give life to injured brain neurons

10:10 am BREAK

10:30 am STEM CELLS AND REGENERATIVE MEDICINEChairs: Andras Nagy (UofT) & Anna Falk (KI)

Urban Lendahl (KI): Deregulated Notch signaling in diseaseGordon Keller (UofT): Modeling human development and disease with pluripotent stem cellsAndras Nagy (UofT): Pluripotency in the artificial cell spaceKenneth Chien (KI): A blueprint for human heart regeneration

12:15 pm LUNCH AND POSTERS

13:45 pm TRANSPLANTATION AND TISSUE ENGINEERINGChairs: Atul Humar (UofT) & Urban Lendahl (KI)

Shaf Keshavjee (UofT): Ex vivo repair of lungs for transplantationStephen Strom (KI): Stem cell therapy of liver diseaseMolly Shoichet (UofT): Biomaterial Strategies for Cell DeliveryAna Teixeira (KI): Spatial signaling in cell-to-cell communication

3:25 pm CLOSING REMARKSCatharine Whiteside, Former Dean of Medicine, University of Toronto

3:30 pm CLOSE OF SYMPOSIUM

Chairs and Speakers

Klas Blomgren, Director, Unit of Neonatology, Professor and Deputy Head of the Department of Women’s and Children’s Health (KBH), Karolinska Institutet

Kenneth Chien, Professor, Department of Medicine and Department of Cell and Molecular Biology (CMB), Karolinska Institutet

Anna Falk, Associate Professor, Department of Neuroscience, Karolinska Institutet

Ola Hermanson, Scientific Coordinator of Karolinska Institutet relations with Canada, Associate Professor, Senior Researcher, Department of Neuroscience, Karolinska Institutet

Atul Humar, Professor of Medicine, University of Toronto, Director, University of Toronto Transplant Institute, Director, Multi Organ Transplant Program R. Fraser Elliott Chair in Transplantation, University Health Network

Gordon Keller, Professor, Medical Biophysics, University of Toronto, Director, McEwen Centre for Regenerative Medicine, University Health Network

Shaf Keshavjee, Professor, Division of Thoracic Surgery and Institute of Biomaterials and Biomedical Engineering, University of Toronto, Surgeon in Chief, Sprott Department of Surgery, University Health Network, James Wallace McCutcheon Chair in Surgery, Director Toronto Lung Transplant Program, Director Latner Thoracic Research Laboratories

Urban Lendahl, Director, StratRegen, Wallenberg Institute of Regenera-tive Medicine (WIRM), the Strategic Center in Developmental Biology for Regenerative Medicine (DBRM), Professor, Department of Cell and Molecular Biology (CMB), Karolinska Institute

Stephen G. Matthews, Professor, Physiology, Ob-Gyn and Medicine, and Director of Research, Fraser Mustard Institute of Human Development, University of Toronto

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Chairs and Speakers

Andras Nagy, Professor, Department of Obstetrics & Gynaecology and Institute of Medical Sciences, University of Toronto, Senior Scientist, Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Tier 1 Canada Research Chair in Stem Cells and Regeneration

Janet Rossant, University Professor, Molecular Genetics, University of Toronto, Chief of Research Hospital for Sick Children

Rickard Sandberg, Associate Professor, Ludwig Cancer Research Institute, Department of Cell and Molecular Biology (CMB), Karolinska Institutet

Molly Shoichet, University Professor of Chemical Engineering & Ap-plied Chemistry, Chemistry and Biomaterials & Biomedical Engineering, University of Toronto, Tier 1 Canada Research Chair in Tissue Engineering

Stephen Strom, Torsten och Ragnar Söderberg Professor in Cell Trans-plantation and Regenerative Medicine Professor, Department of Labora-tory Medicine (LABMED), Karolinska Institutet

Ana Teixeira, Senior Researcher, Department of Medical Biochemistry and Biophysics (MBB), Karolinska Institutet

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Posters

1 Emma StrongMolecular Genetics

Symmetrical dose-dependent DNA methylation profiles in children with deletion or duplication of chromosome 7q11.23 Emma Strong, Darci T Butcher, Rajat Singhania, Carolyn B Mervis, Colleen A Morris, Daniel De Carvalho, Rosanna Weksberg, Lucy R Osborne

2 Xiaolin HeCritical Care

Human mesenchymal stromal cells improve survival in murine experimental sepsis via the enhancement of bacteria clearance Interdepart-mental Xiaolin He

3 Yimu ZhaoChemical Engineering and Applied Chemistry

Long term monitoring platform for cardiotoxic-ity with engineered cardiac tissue Yimu Zhao, Milica Radisic

4 Sharareh ShojaiePhysiology

ESC-Derived Airway Epithelial Cell Differentia-tion Progresses Through a Basal Cell Progenitor Stage on Decellularized Lung Scaffolds Sharareh Shojaie, Cameron Ackerley, Jinxia Wang, Martin Post

5 Amy Hyemin OhInstitute of Medical Science

Effects of changes in GTF2I and GTF2IRD1 copy number on the neural development in the mouse models of Williams-Beuren Syndrome and 7Q11.23 Duplication Syndrome. Hyemin, Osborne, Lucy

6 Frances WongPhysiology

Transient and Dynamically Regulated EpCAM+ and CDCP1+ Trophoblast Populations in Early Human Placentas Frances Wong and Brian Cox

7 Ursula Nosi Physiology

microRNAs govern trophoblast stem cell fate via repression of embryonic gene regulatory net-works Nosi, U., Cox,

8 Issaka YougbareLaboratory medicine and Pathobiology

Pathology of placenta in fetal and neonatal immune thrombocytopenia: Roles of TH17 immune responses, anti-platelet antibod-ies and angiogenic factors. Issaka Yougbare, Wei-She Tai, Darko Zdravic, Brian Vadasz, Alexandra Marshall, Pingguo Chen, Guangheng Zhu, Howard x Howard Leong-Poi, Dawei Qu, Yu X. Lisa, Lee S. Adamson, John Sled, John Freedman, Heyu Ni

9 Anna BadnerInstitute of Medical Science

The vasoprotective effect of early intravenous mesenchymal cell delivery in traumatic spinal cord injury Anna Badner, Reaz Vawda, Mirriam Mikhail, Alex Laliberte, James Hong and Michael Fehlings

10 Julieta LazarteInstitute of Medical Science

Human leukocyte antigen-g polymorphisms as predictors of early cardiac allograft vasculopathy Julieta Lazarte, Livia Goldraich, Hiroyuki Kawaji-ri, Arash Ghashghai, Liza Grosman-Rimon, Vivek Rao, Diego Delgado

11 Xiao ZhaoOtolaryngology - Head and Neck Surgery

Metabolic reprogramming of radiation fibrosis using adipose derived stromal cells Xiao Zhao, Ju Hee Lee, Laurie Ailles, Kenneth Yip, Fei-Fei Liu

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12 Roshane FrancisMolecular Genetics

The role of Irx3 and Irx5 in the early gastric development Roshane Francis

13 Malgosia PakulskaChemical Engineering

Combined therapy of Chondroitinase ABC and stromal cell-derived factor 1α (SDF1α) for spinal cord regeneration Malgosia Pakulska, Katarina Vulic, Molly S. Shoichet

14 Michelle FrenchPhysiology

Strategies for teaching Regenerative Medicine to undergraduate students Michelle French

15 Ekaterina TurlovaPhysiology

TRPM7 regulates axonal outgrowth and matura-tion of primary hippocampal neurons. Ekaterina Turlova, Christine Youjin Bae, Marielle Deurloo, Wenliand Chen, Andrew Barszczyk, F David Horgen, Andrea Fleig, Zhong-Ping Feng and Hong-Shuo Sun

16 Mathew WongPhysiology

Statin-mediated modulation of pulmonary RhoA/ROCK signalling in experimental chronic neonatal pulmonary hypertension. Mathew Wong, Julijana Ivanovska, Crystal Kantores, Robert P Jankov.

17 Marielle Deurloo Physiology

General Transcription Factor 2I (Gtf2i) copy number regulates neuronal maturation and cognition through TRPC3 channel and mus-carinic receptor-dependent mechanisms. Marielle H.S. Deurloo, Wenliang Chen, JacquelineN Crawley, Hong-Shuo Sun, Lucy R. Osborne, Zhong-Ping Feng

18 Brian BalliosFaculty of Medicine

An injectable hydrogel improves the survival and functional integration of stem cell prog-eny transplanted into adult retina Brian G. Ballios, Michael J. Cooke, Laura Clarke, Brenda L.K. Coles, Cindi M. Morshead, Derek van der Kooy, Molly S. Shoichet

19 Vasilis MoisiadisPhysiology

Antenatal Glucocorticoids lead to Multigen-erational Programming of Adult Behaviour and HPA Function via Paternal Transmission. Vasilis G. Moisiadis, Alisa Kostaki and Stephen G. Matthews

Posters

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