aav9-cmv- egfp mir122(3x)ts
DESCRIPTION
AAV9-CMV- EGFP miR122(3x)TS. AAV9-CMV-MLC0.26- EGFP miR122(3x)TS. AAV9-CMV- EGFP. AAV9-CMV-MLC0.26- EGFP. heart. Supplementary Figure I. AAV9-CMV- EGFP miR122(3x)TS. AAV9-CMV-MLC0.26- EGFP miR122(3x)TS. AAV9-CMV- EGFP. AAV9-CMV-MLC0.26- EGFP. Quadriceps femoris muscle. - PowerPoint PPT PresentationTRANSCRIPT
AAV9-CMV-EGFPmiR122(3x)TS
AAV9-CMV-EGFP
AAV9-CMV-MLC0.26-EGFPmiR122(3x)TS
AAV9-CMV-MLC0.26-EGFP
hea
rt
Supplementary Figure I
Qu
adri
cep
sfe
mo
ris
mu
scle
AAV9-CMV-EGFPmiR122(3x)TS
AAV9-CMV-EGFP
AAV9-CMV-MLC0.26-EGFPmiR122(3x)TS
AAV9-CMV-MLC0.26-EGFP
Supplementary Figure II
AAV9-CM
V-
EGFP m
iR12
2(3x
)TS
AAV9-CM
V-
EGFP
AAV9-CM
V-MLC0.
26-
EGFP m
iR12
2(3x
)TS
AAV9-CM
V-
MLC0.
26-E
GFP
AAV9-CM
V-
EGFP m
iR12
2(3x
)TS
AAV9-CM
V-
EGFP
AAV9-CM
V-MLC0.
26-
EGFP m
iR12
2(3x
)TS
AAV9-CM
V-
MLC0.
26-E
GFP
Supplementary Figure III
heart
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8.0
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13.0
C
t (v
ec
tor
vs
. G
AP
DH
)
liver
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
C
t (v
ec
tor
vs
. G
AP
DH
)
Supplementary Figure Legends
Supplementary Figure I:
Regulation of AAV9 vector mediated gene expression by miR122 in the heart does not affect
transduction of cardiomyocytes. Representative microphotographs show colocalization of EGFP
fluorescence with an actin stain using phalloidin (red). The pattern of EGFP expression in the heart is similar
between all groups irrespective of the use of miR122 TS. Bar: 50 µm.
Supplementary Figure II:
Visualization of EGFP expression in skeletal muscle. Cryosections of quadriceps femoris muscle from
adult mice 4 weeks after intravenous injection of 7.5x1011 vg of AAV9-CMV-EGFP, AAV9-CMV-EGFPmiR122(3x)TS,
AAV9-CMV-MLC0.26-EGFP, and AAV9-CMV-MLC0.26-EGFPmiR122(3x)TS were compared. Expression was found
to be close to the detection limit. No difference in expression was detectable either with or without miR122 TS
in combination with the CMV or CMV-MLC0.26 promoter. Bar: 50 µm
Supplementary Figure III:
Comparison of AAV vector DNA amount in vivo. Vector DNA of AAV9-CMV-EGFP, AAV9-CMV-
EGFPmiR122(3x)TS, AAV9-CMV-MLC0.26-EGFP and AAV9-CMV-MLC0.26-EGFPmiR122(3x)TS in heart and liver was
quantified by real-time PCR and related to GAPDH DNA. Shown are Ct values of vector DNA vs. GAPDH
DNA, the mean and standard error of mean (SEM). The vector DNA levels measured in the heart and the liver
were very similar between all groups.