abacard p30_semen_miami dade police_adriana kristaly_david smith

7/21/2019 ABAcard p30_Semen_Miami Dade Police_Adriana Kristaly_David Smith

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Validation of the ne tep B cardTMPS test for the rapid

forensic identification of semen

Adriana Kristaly, BS and David A.S. Smith, MSFS, Forensic Biology Section, Crime

Laboratory Bureau, Miami-Dade Police Department, Miami, Florida.

The visualization of spermatozoa is the most reliable forensic identifier of semen. This

method is obviously ineffective when examining semen from azoospermic or

vasectomized males. Forensic identification of this type of evidence has been

traditionally accomplished by the detection of a unique seminal protein called Prostate

Specific Antigen (PSA) also lmown as p30. The methods used to detect p30 include

immunodiffusion, crossover immunoelectrophoresis and rocket immunoelectrophoresis.

All of these methods are rather complex and time consuming. In this presentation we

examine the

ne tep

ABAcard p30 detection system, a simple and rapid method for the

forensic identification of semen. This poster will present results ITom various studies

including sensitivity, sample size, body fluid mixtures, azoospermic semen and

environmental insults.

The

ne tep

ABAcard PSA test proved to be an effective, sensitive, simple and rapid

method for the forensic identification of semen.

Introduction

The identification of seminal fluid in the absence of spermatozoa is of forensic

importance. This can be accomplished by the detection of unique seminal proteins such

as prostate specific antigen (PSA), also lmown as p30. This is a semen glycoprotein of

prostatic origin with a concentration range of2.0xlO5 to 5.5xlO6nanograms per milliliter

of semen, The

ne tep

ABAcardTMPSA test is designed to qualitatively detect p30 that

could include azoospermic and vasectomized individuals.

In the ABAcardTMPSA test procedure, the p30 antigen reacts with the mobile (dye

particle linked) monoclonal antihuman p30 antibody forming a mobile antigen-antibody

complex, This mobile complex then migrates towards the test ( T ) area where an

immobilized polyclonal antihuman p30 antibody is located. An antibody-antigen-

antibody sandwich is formed when the immobilized antibody captures the above

complex. Ifp30 is present at or above 4 ng/ml in a sample, a pink colored band will form

in the test T area. An internal positive control ( C ) area indicates that the test has

worked properly and proper procedures have been followed. The migrating p30 antibody-

dye complex will not bind to the antibody in the T area but will bind to an immobilized

anti-immunoglobulin antibody in the control c area. The captured pink dye particles

will form a pink band in this area. A single pink colored line in the control c area

indicates a negative result, while two colored lines, one in the test T area and another in

the control c area indicate a positive result (provided no High Dose Hook Effect ).


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abacard p30_semen_miami dade police_adriana kristaly_david smith

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