An economical, versatile platformfor DNA synthesis.

ABI 3900 High-Throughput DNA Synthesizer

AB

I 3900H

igh-Throughput D

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Synthesizer

High-quality oligonucleotide primers and probes.

The ABI 3900 High-Throughput DNA Synthesizer is an economical, versatile

platform that synthesizes DNA oligos with rapid cycle times, low reagent

consumption, and incomparable reliability. As the applications of genetic

analysis, PCR-based, and other probe-based hybridization assays expand,

so does the need for oligos. The solution: the ABI 3900 Synthesizer—

designed for production-level environments, where the need for flexibility

is high, tolerance for down-time is low, and bench space is limited.

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Overhead view of reagent delivery systemand column cartridge chamber.

A Perfect Balance

The ABI 3900 Synthesizer is an economical and versatile platform that is ideal for synthesizing primers and probes, fluorescently-labeled oligos, and production synthesis. It fits compactly in most workspaces, and easily synthesizessequences with lengths greater than 50-mer at scales of 40 nmol, 0.2 µmol, and 1 µmol.

High-Throughput Capacity

The ABI 3900 Synthesizer features parallel, unattended 48-column synthesis and has 40 and 200 nmol cycle times

of less than 5 minutes for 48 couplings. The instrument hasthe ability to synthesize up to 288 40 and 200 nmol scale oligos (20-mers) in a ten-hour workday with minimal user

intervention (excludes cleavage and deprotection).

Everything You Need

Applied Biosystems provides a full line of instrument accessories, chemistries, and consumables exclusively for the ABI 3900 High-Throughput DNA Synthesizer.

Optimized Chemistry

The 3900 system uses Applied Biosystems proprietary

FastPhoramidite® ß-cyanoethyl chemistry. The FastPhoramidite®

reagent set is distinguishable from the standard set by theprotecting group on the G monomer. In the FastPhoramidite

set, the more labile dimethylformamide replaces the isobutryl

group and allows the removal of the protecting group in justone hour at 65°C.

Superior Columns

3900 DNA synthesis is performed on unique polystyrene-filled ABI 3900HT Columns in scales of 40 nmol, 0.2 µmol,

and 1 µmol. Polystyrene columns and supports have advantages over conventional controlled pore glass (CPG)

columns. The greater hydrophobicity of polystyrene results

in a higher yield of the full-length product. Side reactions,such as extraneous chain growth, are minimized by the inertnature of this support surface.

Simple Design

The ABI 3900 synthesizer has 14 amidite bottle positions

located in the front panel where they are easily accessible.Reagents are positioned in the side panel for added convenience.

Applied Biosystems Quality and Support

Customer-site installation by a service representative isincluded. A software and synthesis training package, and one-year warranty on parts and labor, are also included with system purchase.

The ABI 3900 High-Throughput DNA Synthesizer is sup-ported by Applied Biosystems service organizations, includingcomprehensive technical support and instrument service.

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ABI 3900 HT columns packaged in containers of 200, color-codedgreen, yellow, red, and blue for A, G, C, and T, respectively.

Close-up view of the ABI 3900 columns in the cartridge dividedinto four banks of twelve.

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iScience. To better understand the complex interaction of biological processes, life scientists are developing revolutionary approaches to discovery that unite technology, informatics,and traditional laboratory research. In partnershipwith our customers, Applied Biosystems provides the innovative products, services, and knowledge resources that make this new, Integrated Science possible.

Worldwide Sales Offices

Applied Biosystems vast distribution and servicenetwork, composed of highly trained support andapplications personnel, reaches 150 countries onsix continents. For international office locations,please call the division headquarters or refer to our Web site at www.appliedbiosystems.com

Applera is committed to providing the world’s leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses.

Headquarters850 Lincoln Centre Drive Foster City, CA 94404 USA Phone: 650.638.5800Toll Free: 800.345.5224Fax: 650.638.5884

For Research Use Only.Not for use in diagnostic procedures.

Applied Biosystems and FastPhoramidite are registered trademarks, and AB (Design), iScience, and iScience (Design) are trademarks of Applera Corporation and/or its subsidiaries in the US and certain other countries.

Dell is a trademark of Dell Corporation.

Applied Biosystems develops and manufactures its products inaccordance with ISO 9000 quality system requirements.

©2004 Applied Biosystems. All rights reserved.Specifications subject to change without notice.

Printed in the USA, 12/04, P+s,Publication 108BR02-01

Applied Biosystems performance. For more information on the ABI 3900 High-Throughput DNA Synthesizer, as well as our

industry-leading phosphoramidite synthesis chemistries, consumables, and full range of

custom oligo synthesis services, contact your local Applied Biosystems representative

or visit the Applied Biosystems Web site at www.appliedbiosystems.com

SpecificationsOligo Yields 5–10 ODU/20-mer synthesis at 40 nmol scale

20–80 ODU/20-mer synthesis at 0.2 µmol scale100 ODU/20-mer synthesis at 1 µmol scale

Bottle Configuration 14 amidite bottle positionsTwo 450 mL reservoirs for each ancillary reagent, including Cap A, Cap B, Oxidizer, and Activator solutionsOne 2 L bottle for Deblock solution, two 4 L acetonitrile bottles Two banks of seven phosphoramidite bottle positions for maximum flexibility One 20 L carboy waste container

Computer Dell™ Business Line computer. For the latest computer specifications,visit www.appliedbiosystems.com

Instrument Dimensions Height: 54.0 cm (21.25 in.)Width: 54.6 cm (21.50 in.)Depth: 54.0 cm (21.25 in.)Weight: 93 kg (205 lbs)

Power Requirement 100–120 or 220–230 V (AC), 50 or 60 Hz

Power Supply Switching power supply with auto-voltage selection (dedicated 1.25 kVA power line)

Power Consumption 250 W

UL and CE Approved UL 3101 (for UL mark) CSA 1010 (for UL and cUL marks)EN 61010-1 (for CE marks)EN 61326-1 (which includes IEC 1000-3-2, 1000-3-3, etc.; and EN 50082-1 and EN 55011 Class B)

Warranty One year

Ordering InformationDescription Quantity P/NABI 3900 High-Throughput DNA Synthesizer 4318174

AccessoriesColumn Insertion Tool 1 4331623Column Removal Tool 1 4324169Column Strips 100 4332220Column Plugs 50 432407296-Well Column Adaptor 1 4324172Cleavage and Deprotection Apparatus 1 4332808

ABI 3900HT ColumnsdAbz (40 nmol) 200/Pkg. 4316671dAbz (0.2 µmol) 200/Pkg. 4316675dAbz (1 µmol) 200/Pkg. 4316679dGdmf (40 nmol) 200/Pkg. 4316673dGdmf (0.2 µmol) 200/Pkg. 4316677dGdmf (1 µmol) 200/Pkg. 4316681dCbz (40 nmol) 200/Pkg. 4316672dCbz (0.2 µmol) 200/Pkg. 4316676dCbz (1 µmol) 200/Pkg. 4316680T (40 nmol) 200/Pkg. 4316674T (0.2 µmol) 200/Pkg. 4316678T (1 µmol) 200/Pkg. 4316682